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Conserved domains on  [gi|489818944|ref|WP_003722757|]
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MULTISPECIES: tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL [Listeria]

Protein Classification

tRNA (cytidine(34)-2'-O)-methyltransferase( domain architecture ID 10000715)

tRNA (cytidine(34)-2'-O)-methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine to the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA)

EC:  2.1.1.207
Gene Ontology:  GO:0003723|GO:0008173|GO:1904047|GO:0141098|GO:0001510
SCOP:  4002340

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 2-156 9.70e-99

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


:

Pssm-ID: 439989  Cd Length: 153  Bit Score: 281.15  E-value: 9.70e-99
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   2 PNHIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNEGGEFYYITK 81
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGGRLFLFTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489818944  82 FGRHVYSDIDYSDtnkDYFFVFGKETTGLPDALLQENEENCLRIPMTDHIRSLNLSNTAAILAYEALRQQNFGAL 156
Cdd:COG0219   81 KGTKPYTDVDYQP---GDVLLFGRETAGLPEEVLAAPADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
 
Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 2-156 9.70e-99

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 281.15  E-value: 9.70e-99
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   2 PNHIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNEGGEFYYITK 81
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGGRLFLFTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489818944  82 FGRHVYSDIDYSDtnkDYFFVFGKETTGLPDALLQENEENCLRIPMTDHIRSLNLSNTAAILAYEALRQQNFGAL 156
Cdd:COG0219   81 KGTKPYTDVDYQP---GDVLLFGRETAGLPEEVLAAPADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 4-151 4.29e-92

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 263.93  E-value: 4.29e-92
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   4 HIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNEGGEFYYITKFG 83
Cdd:cd18094    1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALPPGRLFLFTTKG 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489818944  84 RHVYSDIDYSDtnkDYFFVFGKETTGLPDALLQENEENCLRIPMTDHIRSLNLSNTAAILAYEALRQQ 151
Cdd:cd18094   81 TKPYTDFDFKP---GDYLVFGRESTGLPEEILDAFPDRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 4-153 4.32e-72

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 213.82  E-value: 4.32e-72
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944    4 HIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNEGGEFYYITKFG 83
Cdd:TIGR00185   3 NIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEKPQKLFALTKKG 82

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   84 RHVYSDIDYSDTNkdyFFVFGKETTGLPDALLQENEENCLRIPMTDHIRSLNLSNTAAILAYEALRQQNF 153
Cdd:TIGR00185  83 TPAHSAVTYQDGD---YLMFGPETRGLPQSILDNLMEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGY 149
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 4-158 3.50e-41

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 135.51  E-value: 3.50e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   4 HIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNEGGEFYYITKFG 83
Cdd:PRK10358   3 NIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQRLFALTTKG 82

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 489818944  84 RHVYSDIDYSDTNkdyFFVFGKETTGLPDALLQE-NEENCLRIPMTDHIRSLNLSNTAAILAYEALRQQNF-GALLQ 158
Cdd:PRK10358  83 TPAHSAVSYQDGD---YLMFGPETRGLPASILDAlPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYpGAVLR 156
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 2-145 1.37e-36

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 123.41  E-value: 1.37e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944    2 PNHIVLYQPEIPANTGNISRTCAGTDTY-LHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFE--KNEGGEFYY 78
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGADgLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKdlKEAGYAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 489818944   79 ITKFGRHVYsDIDYsdtNKDYFFVFGKETTGLPDALLQENEEnCLRIPMTDHIRSLNLSNTAAILAY 145
Cdd:pfam00588  81 TSLGGKSLY-EIDF---PKKVALVFGNEGTGLSNEVLELCDR-LVRIPMSGDVESLNVAVAAAIALY 142
 
Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 2-156 9.70e-99

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 281.15  E-value: 9.70e-99
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   2 PNHIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNEGGEFYYITK 81
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGGRLFLFTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489818944  82 FGRHVYSDIDYSDtnkDYFFVFGKETTGLPDALLQENEENCLRIPMTDHIRSLNLSNTAAILAYEALRQQNFGAL 156
Cdd:COG0219   81 KGTKPYTDVDYQP---GDVLLFGRETAGLPEEVLAAPADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 4-151 4.29e-92

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 263.93  E-value: 4.29e-92
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   4 HIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNEGGEFYYITKFG 83
Cdd:cd18094    1 NIVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALPPGRLFLFTTKG 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489818944  84 RHVYSDIDYSDtnkDYFFVFGKETTGLPDALLQENEENCLRIPMTDHIRSLNLSNTAAILAYEALRQQ 151
Cdd:cd18094   81 TKPYTDFDFKP---GDYLVFGRESTGLPEEILDAFPDRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 4-153 4.32e-72

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 213.82  E-value: 4.32e-72
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944    4 HIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNEGGEFYYITKFG 83
Cdd:TIGR00185   3 NIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEKPQKLFALTKKG 82

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   84 RHVYSDIDYSDTNkdyFFVFGKETTGLPDALLQENEENCLRIPMTDHIRSLNLSNTAAILAYEALRQQNF 153
Cdd:TIGR00185  83 TPAHSAVTYQDGD---YLMFGPETRGLPQSILDNLMEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGY 149
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 4-158 3.50e-41

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 135.51  E-value: 3.50e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   4 HIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNEGGEFYYITKFG 83
Cdd:PRK10358   3 NIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQRLFALTTKG 82

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 489818944  84 RHVYSDIDYSDTNkdyFFVFGKETTGLPDALLQE-NEENCLRIPMTDHIRSLNLSNTAAILAYEALRQQNF-GALLQ 158
Cdd:PRK10358  83 TPAHSAVSYQDGD---YLMFGPETRGLPASILDAlPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYpGAVLR 156
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 2-145 1.37e-36

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 123.41  E-value: 1.37e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944    2 PNHIVLYQPEIPANTGNISRTCAGTDTY-LHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFE--KNEGGEFYY 78
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGADgLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKdlKEAGYAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 489818944   79 ITKFGRHVYsDIDYsdtNKDYFFVFGKETTGLPDALLQENEEnCLRIPMTDHIRSLNLSNTAAILAY 145
Cdd:pfam00588  81 TSLGGKSLY-EIDF---PKKVALVFGNEGTGLSNEVLELCDR-LVRIPMSGDVESLNVAVAAAIALY 142
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 4-147 4.33e-32

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 111.93  E-value: 4.33e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   4 HIVLYQPEIPANTGNISRTCAGTD-TYLHLIRPLGFSTDDKMlKRAGLDYWDNVKLSYYDSLDEFFE--KNEGGEFYY-- 78
Cdd:cd18082    1 HLVLDNIRSPGNIGSIFRTADAFGvEKIYLVGPTADIFDPKA-KRTSRGTHFSVPYERVDNFEEYLKslKEEGYPLIGle 79

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489818944  79 ITKFGRHVYsDIDYSDTNKDYFfVFGKETTGLPDALLQENEEnCLRIPMTDHIRSLNLSNTAAILAYEA 147
Cdd:cd18082   80 LTDGSIPLS-EFKFKKPKKIAL-IFGNERSGLSPELLALCDY-TVHIPMSGKNSSLNVSVATGIALYEF 145
SpoU-like_RNA-MTase cd18104
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 5-151 1.22e-13

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349977  Cd Length: 146  Bit Score: 64.52  E-value: 1.22e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   5 IVLYQPEIPANTGNISRTC--AGTD--------TYLH---LIRPlgfSTddkmlkraGLDYWDNVKLSYYDSLDEFFEKN 71
Cdd:cd18104    3 LVLEGLEKPGNLGAILRSAdaAGADavilcdpkTDLFnpnVIRA---SL--------GAVFTLPVAEASSEETLAWLKEN 71

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944  72 EggefyyITKFGRHVYSDIDYSDTN--KDYFFVFGKETTGLPDALLQENEENcLRIPMTDHIRSLNLSNTAAILAYEALR 149
Cdd:cd18104   72 K------IRILATTPKAEKLYYEADlrGPIAIVVGSESEGLSEFWLKAADER-VRIPMAGQADSLNVSVAAAILLYEALR 144


                 ..
gi 489818944 150 QQ 151
Cdd:cd18104  145 QR 146
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 83-154 2.94e-11

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 59.82  E-value: 2.94e-11
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489818944  83 GRHVYSDIDYSdtnKDYFFVFGKETTGLPDALLqeneENC---LRIPMTDHIRSLNLSNTAAILAYEALRQQNFG 154
Cdd:COG0566  198 GEASLYEVDLP---GPVALVLGNEGKGLSRLVL----EACdelVRIPMAGGVESLNVAVAAAILLYEAARQRRAK 265
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 13-147 1.57e-10

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 55.90  E-value: 1.57e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944  13 PANTGNISRTCAGtdtylhlirpLGFST-----------DDKMLkRAGLDYWDNVKLSYYDSLDEFFE--KNEGGEFYYI 79
Cdd:cd18095   11 PGNLGTIIRTAAA----------FGVDGvilskgtvdpyNPKVV-RASMGALFRLPIIRVDDLEELLEelKEAGYKVVAA 79

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489818944  80 TKFGRHVYSDIDYsdtNKDYFFVFGKETTGLPDALLQENEENcLRIPMTDHIRSLNLSNTAAILAYEA 147
Cdd:cd18095   80 DLDGGSSLYDLDL---PKPVALVLGNEGNGLSDELLELCDKR-VTIPMSGGVESLNVAVAAAILLYEL 143
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 4-146 2.17e-10

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 55.96  E-value: 2.17e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   4 HIVLYQPEIPANTGNISRTCAGTD-TYLHLIRPLGFSTDDKMLKRA--GLDYWDNVKLsyYDSLDEFFEkneGGEFYYIT 80
Cdd:cd18093    2 RVVLVEPQHPGNIGAVARAMKNFGlSDLRLVNPRCDDPEEAARALAsgAEDVLENAKV--YDTLEEALA---DCDLVVAT 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944  81 -----KFGRHVYS------DIDYSDTNKDYFFVFGKETTGLpdallqENEE--NC---LRIPMTDHIRSLNLSNTAAILA 144
Cdd:cd18093   77 tarprGLRRPLLTprelaeLLKEHAAGGKVALVFGREDSGL------TNEElaLCdllVTIPTNPEYPSLNLAQAVAIVL 150


                 ..
gi 489818944 145 YE 146
Cdd:cd18093  151 YE 152
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 11-150 2.02e-09

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 53.63  E-value: 2.02e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944  11 EIPANTGNIS---RTCagtD----TYLHLIRPlgfstDDKMLKR----AGLDYWDNVKLsyYDSLDEFFE--KNEGgefY 77
Cdd:cd18092   22 ENVHDPHNASavlRTC---DafgvQDVHVIEP-----KNKFKKNrgvaMGADKWLDVHR--HDSTEECLAalKEQG---Y 88

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 489818944  78 YItkFGRHVYSD-IDYSDtnKDYF----FVFGKETTGLPDALLQENEEnCLRIPMTDHIRSLNLSNTAAILAYEALRQ 150
Cdd:cd18092   89 RI--VATSLSPDaVPLDE--LDYTrptaLVFGNEKDGVSEEALAAADE-RVYIPMYGFVESLNVSVAAAIILYELQRQ 161
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 5-151 4.19e-09

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 53.58  E-value: 4.19e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   5 IVLYQPEIPANTGNISRTCA--G-TDtyLHLIRPLGFStDDKMLKRA--GLDYWDNVKLsyYDSLDEFFEkneggEFYYI 79
Cdd:COG0565    6 VVLVEPQHPGNIGAAARAMKnfGlSD--LRLVNPRGWP-DEEARAMAsgADDVLENARV--VDTLEEALA-----DCDLV 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944  80 --T-----KFGRHVYS------DIDYSDTNKDYFFVFGKETTGLpdallqENEE--NC---LRIPMTDHIRSLNLSNTAA 141
Cdd:COG0565   76 vgTtarprELRKPVLTpreaaeELLARAAGGKVALVFGRERSGL------TNEElaLCdalVTIPTNPEYPSLNLAQAVQ 149

                        170
                 ....*....|
gi 489818944 142 ILAYEaLRQQ 151
Cdd:COG0565  150 VVAYE-LRKA 158
SpoU-like_RlmB cd18103
SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the ...
 87-147 2.54e-07

SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349976  Cd Length: 143  Bit Score: 47.38  E-value: 2.54e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 489818944  87 YSDIDYsdtNKDYFFVFGKETTGLPDALLqeneENC---LRIPMTDHIRSLNLSNTAAILAYEA 147
Cdd:cd18103   87 LYDVDL---TGPLALVLGSEGKGLRRLVL----KNCdflVKIPMAGKVDSLNVSVAAGIILYEI 143
SpoU-like_TRM3-like cd18091
SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O) ...
 102-146 9.94e-07

SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O)-methyltransferase TRM3 catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in various tRNAs. TRM3 is similar to C-terminal domain of TAR (HIV-1) RNA binding protein 1 (TARBP1), a protein binding to TAR, which functions as a RNA regulatory signal by forming a stable stem-loop structure to which transactivator protein Tat binds. The role of TARBP1 is believed to be to disengage RNA polymerase II from TAR during transcriptional elongation. TRM3 and the C-terminal methyltransferase domain of TARBP1 are members of the SPOUT methyltransferase superfamily.


Pssm-ID: 349964  Cd Length: 145  Bit Score: 45.98  E-value: 9.94e-07
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 489818944 102 VFGKETTGLPDALLQENEEnCLRIPMTDHIRSLNLSNTAAILAYE 146
Cdd:cd18091  101 VLGNEKEGIPAELLSLLDV-CVEIPQLGVIRSLNVHVSGAIAIYE 144
SpoU-like cd18097
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 4-147 1.03e-06

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349970  Cd Length: 144  Bit Score: 45.63  E-value: 1.03e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   4 HIVLYQPEIPANTGNISRTC--AGTDTyLHL----IRPlgfsTDDKMLKRA-GLDYWdnVKLSYYDSLDEFFE--KNEGg 74
Cdd:cd18097    2 VVVLDNLRSAHNVGSIFRTAdaAGVEK-IYLcgytPTP----PHPKVAKTAlGAEES--VPWEHFEDTLEAVRalKARG- 73

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 489818944  75 efYYI----TKFGRHVYSDIDYSdtnKDYFFVFGKETTGLPDALLQENEeNCLRIPMTDHIRSLNLSNTAAILAYEA 147
Cdd:cd18097   74 --YTIialeTTENSVPLHEARLP---RPLALVLGNEVRGVSPEVLALCD-AVVEIPMFGRKNSLNVAVAAAVALYEL 144
SpoU-like_RNMTL1 cd18106
SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) ...
 85-147 1.19e-06

SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) catalyzes the methylation of 2'-O-ribose residues G1370 to GmG residue of the mitochondrial 16S rRNA. RNMTL1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349979  Cd Length: 148  Bit Score: 45.71  E-value: 1.19e-06
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 489818944  85 HVYSDIDYSDTNkdYFFVFGKETTGL-PDALLQENEENCLR--IPMTDHIRSLNLSNTAAILAYEA 147
Cdd:cd18106   85 QPYDSIDYCSGP--IVLVIGGETHGVsPEAYKLAHSTGGSRvhIPLANGVDSLNSAVAASILLFEM 148
rRNA_methyl_3 TIGR00186
rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases ...
 56-150 1.31e-04

rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 129290 [Multi-domain]  Cd Length: 237  Bit Score: 40.79  E-value: 1.31e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   56 VKLSYYDSLDEFFEK-NEGGEFYYITKfgrHVYSDIDY-SDTNKDYFFVFGKETTGLpDALLQENEENCLRIPMTDHIRS 133
Cdd:TIGR00186 144 VPLARVTNLSRTITKlKESGFWTVGTD---LDAQDTLYqVKLTKPLALVVGNEGEGV-SRLIKENCDFLIKIPMAGKVDS 219

                          90
                  ....*....|....*..
gi 489818944  134 LNLSNTAAILAYEALRQ 150
Cdd:TIGR00186 220 LNVSVAAGILLFEIKRQ 236
rRNA_methyl_1 TIGR00050
RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of ...
 5-161 2.39e-04

RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases, and is now characterized, in E. coli, as a tRNA:Cm32/Um32 methyltransferase. It may be named TrMet(Xm32), or TrmJ, according to the nomenclature style chosen [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272876 [Multi-domain]  Cd Length: 233  Bit Score: 40.08  E-value: 2.39e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944    5 IVLYQPEIPANTGNISRTCAGTD-TYLHLIRPLGFSTDDKMLKRAGL-DYWDNVKLsyYDSLDEFFeknegGEFYYI--- 79
Cdd:TIGR00050   6 IVLVEPSHPGNIGSIARAMKNMGlTELRLVNPRVKLEEEAYALAAGArDILDNAKV--VDTLDEAL-----DDCDFVvgt 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489818944   80 TKFGRHVYSD-IDYSD-------TNKDYFFVFGKETTGLpdallqENEE--NC---LRIPMTDHIRSLNLSNTAAILAYE 146
Cdd:TIGR00050  79 SARSRNLQRPmLTPRElapklvaEKGKVALVFGREDSGL------TNEElqKChvlVSIPTSEEYPSLNLSHAVAVILYE 152

                         170
                  ....*....|....*
gi 489818944  147 aLRQQNfgaLLQEPN 161
Cdd:TIGR00050 153 -LRMAW---LAQQEN 163
PRK10864 PRK10864
putative methyltransferase; Provisional
 102-151 2.52e-04

putative methyltransferase; Provisional


Pssm-ID: 236779 [Multi-domain]  Cd Length: 346  Bit Score: 40.15  E-value: 2.52e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 489818944 102 VFGKETTGLPDALLQENEEnCLRIPMTDHIRSLNLSNTAAILAYEALRQQ 151
Cdd:PRK10864 296 VLGQEYDGLSDAARQQGDL-SVSIDGTGNVESLNVSVATGVLLAEWWRQN 344
PRK11081 PRK11081
tRNA guanosine-2'-O-methyltransferase; Provisional
 87-154 3.56e-04

tRNA guanosine-2'-O-methyltransferase; Provisional


Pssm-ID: 236837 [Multi-domain]  Cd Length: 229  Bit Score: 39.59  E-value: 3.56e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489818944  87 YSDIDYSdtnKDYFFVFGKETTGLPDALLQENEENCLrIPMTDHIRSLNLSNTAAILAYEALRQ-QNFG 154
Cdd:PRK11081 105 FREIDYT---RPTCILMGQEKTGISQEALALADQDII-IPMIGMVQSLNVSVASALILYEAQRQrQNAG 169
SpoU-like_AviRb cd18107
SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes ...
 102-147 7.62e-04

SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA. AviRb is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349980  Cd Length: 148  Bit Score: 37.75  E-value: 7.62e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 489818944 102 VFGKETTGLpDALLQENEENCLRIPMTDHIRSLNLSNTAAILAYEA 147
Cdd:cd18107  104 LIGNETAGL-SAAWRELCDETVSIPMAGSASSLNAACAASIFLYEA 148
SpoU-like_NHR cd18108
Nosiheptide-resistance methyltransferase (NHR); Nosiheptide-resistance methyltransferase (NHR) ...
 102-146 9.72e-03

Nosiheptide-resistance methyltransferase (NHR); Nosiheptide-resistance methyltransferase (NHR) confers resistance to the thiazole antibiotic nosiheptide via catalyzing 2'O-methylation of 23S rRNA at the nucleotide A1067. NHR is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349981  Cd Length: 144  Bit Score: 34.76  E-value: 9.72e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*
gi 489818944 102 VFGKETTGlPDALLQENEENCLRIPMTDHIRSLNLSNTAAILAYE 146
Cdd:cd18108  100 VFGSEKGG-PSGLFEEAAAGTVSIPMSSSTESLNVSVSAGIALHE 143
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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