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Conserved domains on  [gi|489819221|ref|WP_003723033|]
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M56 family metallopeptidase [Listeria monocytogenes]

Protein Classification

M56 family metallopeptidase( domain architecture ID 12063881)

M56 family metallopeptidase with similarity to integral membrane zinc metallopeptidases BlaR1 and MecR1

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Peptidase_M56 pfam05569
BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which ...
 6-296 7.47e-114

BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which mediate staphylococcal resistance to beta-lactam antibiotics) is regulated by a signal-transducing integral membrane protein and a transcriptional repressor. The signal transducer is a fusion protein with penicillin-binding and zinc metalloprotease domains. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. homologs to this peptidase domain, which corresponds to Merops family M56, are also found in a number of other bacterial genome sequences.


:

Pssm-ID: 428523  Cd Length: 296  Bit Score: 332.51  E-value: 7.47e-114
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221    6 KIFLSMALTSVILIPLVWILQRIFNRYLSKGKIYYSWMIVFFFLTIPFAYF--FFRFAKDSVFMWRNKTEFDGVTSIVVD 83
Cdd:pfam05569   1 LFLLRASIAAAAAVLVVLLLRRPLRRYLGAIWAYALWLIVPLALLAPFIPEsaLSNFTTSAIFMARSKAPINSTAPKVID 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221   84 QSGVVLQHDFKTVFWMTVLDYLWVAWLVIFLLTFIYRIASYRNFKKYVFSGARRVDDLEQLDLLAETMEELNIKRPVELM 163
Cdd:pfam05569  81 DQASPLATPFPVVAWSTILKILLLLWIVGALILLLYMIAAYLVFRQRRVRRLGSLRAHELDDILKEAKEDMGIKRPITIS 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221  164 INPLISSPIFLGLKKNVIVIPDE---TFLNDELHYIFKHELVHCKRKDMYYVWMVQFFTCVYWFNPLMYLMNKRIQMDRE 240
Cdd:pfam05569 161 LSSNIDSPAVLGLWKPRIVLPADfdtRLSGEEIDYILAHELSHLKRGDLIINLLVAVLQCLHWFNPLVHLAFRKIRIDQE 240

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 489819221  241 LACDEAVLATLSKSKYIGYGDTLLSSLAKSGNYKESYVAVSLHENTKALKERLICI 296
Cdd:pfam05569 241 LACDAAVLARLHPHERKEYGRTLLKLLAGPSNHIVPVACVWLAGAKSALKERIMML 296
 
Name Accession Description Interval E-value
Peptidase_M56 pfam05569
BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which ...
 6-296 7.47e-114

BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which mediate staphylococcal resistance to beta-lactam antibiotics) is regulated by a signal-transducing integral membrane protein and a transcriptional repressor. The signal transducer is a fusion protein with penicillin-binding and zinc metalloprotease domains. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. homologs to this peptidase domain, which corresponds to Merops family M56, are also found in a number of other bacterial genome sequences.


Pssm-ID: 428523  Cd Length: 296  Bit Score: 332.51  E-value: 7.47e-114
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221    6 KIFLSMALTSVILIPLVWILQRIFNRYLSKGKIYYSWMIVFFFLTIPFAYF--FFRFAKDSVFMWRNKTEFDGVTSIVVD 83
Cdd:pfam05569   1 LFLLRASIAAAAAVLVVLLLRRPLRRYLGAIWAYALWLIVPLALLAPFIPEsaLSNFTTSAIFMARSKAPINSTAPKVID 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221   84 QSGVVLQHDFKTVFWMTVLDYLWVAWLVIFLLTFIYRIASYRNFKKYVFSGARRVDDLEQLDLLAETMEELNIKRPVELM 163
Cdd:pfam05569  81 DQASPLATPFPVVAWSTILKILLLLWIVGALILLLYMIAAYLVFRQRRVRRLGSLRAHELDDILKEAKEDMGIKRPITIS 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221  164 INPLISSPIFLGLKKNVIVIPDE---TFLNDELHYIFKHELVHCKRKDMYYVWMVQFFTCVYWFNPLMYLMNKRIQMDRE 240
Cdd:pfam05569 161 LSSNIDSPAVLGLWKPRIVLPADfdtRLSGEEIDYILAHELSHLKRGDLIINLLVAVLQCLHWFNPLVHLAFRKIRIDQE 240

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 489819221  241 LACDEAVLATLSKSKYIGYGDTLLSSLAKSGNYKESYVAVSLHENTKALKERLICI 296
Cdd:pfam05569 241 LACDAAVLARLHPHERKEYGRTLLKLLAGPSNHIVPVACVWLAGAKSALKERIMML 296
MecR1 COG4219
Signal transducer regulating beta-lactamase production, contains metallopeptidase domain ...
 109-326 4.13e-53

Signal transducer regulating beta-lactamase production, contains metallopeptidase domain [Signal transduction mechanisms];


Pssm-ID: 443363 [Multi-domain]  Cd Length: 337  Bit Score: 178.32  E-value: 4.13e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221 109 WLVIFLLTFIYRIASYRNFKKYVFSgARRVDDLEQLDLLAETMEELNIKRPVELMINPLISSPIFLGLKKNVIVIPDETF 188
Cdd:COG4219    1 WLAGVLLLLLRLLISLLRLRRLLRR-ARPVTDEELLELLERLARRLGIRRPVRLLESDRITSPFSFGLLRPVILLPAGLE 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221 189 L--NDELHYIFKHELVHCKRKDMYYVWMVQFFTCVYWFNPLMYLMNKRIQMDRELACDEAVLATLSKSKyiGYGDTLLSS 266
Cdd:COG4219   80 ElsEEELEAILAHELAHIRRRDLLDNLLAELLLALFWFNPLVWLARRRLRLDRELACDAAVLKAGGDRK--AYAETLLKL 157

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221 267 LAKSgnyKESYVAVSLHENTKALKERLICIANYETPSKKHNILFLIFIIVLCSVGVLFSA 326
Cdd:COG4219  158 AERR---SQPALALAFGGSKSTLKKRIKMLLKSKSKRRSRLKLLLALLLALLLALALLSA 214
M56_BlaR1_MecR1_like cd07341
Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; ...
 105-299 1.84e-44

Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; This family contains peptidase M56, which includes zinc metalloprotease domain in MecR1 as well as BlaR1. MecR1 is a transmembrane beta-lactam sensor/signal transducer protein that regulates the expression of an altered penicillin-binding protein PBP2a, which resists inactivation by beta-lactam antibiotics, in methicillin-resistant Staphylococcus aureus (MRSA). BlaR1 regulates the inducible expression of a class A beta-lactamase that hydrolytically destroys certain ?-lactam antibiotics in MRSA. Both, MecR1 and BlaR1, are transmembrane proteins that consist of four transmembrane helices, a cytoplasmic zinc protease domain, and the soluble C-terminal extracellular sensor domain, and are highly similar in sequence and function. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. All members contain the zinc metalloprotease motif (HEXXH). Homologs of this peptidase domain are also found in a number of other bacterial genome sequences, most of which are as yet uncharacterized.


Pssm-ID: 320700 [Multi-domain]  Cd Length: 187  Bit Score: 150.94  E-value: 1.84e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221 105 LWVAWLVIFLLTFIYRIASYRNFKKYvfsgARRVDDleqlDLLAETMEELNIKRPVELMINPLISSPIFLGLKKNVIVIP 184
Cdd:cd07341    1 IWLAGALLLLLRLLRGLLRLRRLRRR----AEPVPD----SLLLELARRLGLRRSVRLSVSALVASPMVVGLFRPVILLP 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221 185 DETFLN--DELHYIFKHELVHCKRKDMYYVWMVQFFTCVYWFNPLMYLMNKRIQMDRELACDEAVLATLSKSKyiGYGDT 262
Cdd:cd07341   73 EGLLEGspEELRAILLHELAHIRRRDLLVNLLQRLLEALFWFNPLVWLLSRRLRLERELACDEAVLAALGDKE--DYAEA 150

                        170       180       190
                 ....*....|....*....|....*....|....*..
gi 489819221 263 LLsSLAKSGNYKESYVAVSLHENTKALKERLICIANY 299
Cdd:cd07341  151 LL-RLAERRSQPPPALALALAGSKSLLKRRIKRILKK 186
 
Name Accession Description Interval E-value
Peptidase_M56 pfam05569
BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which ...
 6-296 7.47e-114

BlaR1 peptidase M56; Production of beta-Lactamase and penicillin-binding protein 2a (which mediate staphylococcal resistance to beta-lactam antibiotics) is regulated by a signal-transducing integral membrane protein and a transcriptional repressor. The signal transducer is a fusion protein with penicillin-binding and zinc metalloprotease domains. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. homologs to this peptidase domain, which corresponds to Merops family M56, are also found in a number of other bacterial genome sequences.


Pssm-ID: 428523  Cd Length: 296  Bit Score: 332.51  E-value: 7.47e-114
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221    6 KIFLSMALTSVILIPLVWILQRIFNRYLSKGKIYYSWMIVFFFLTIPFAYF--FFRFAKDSVFMWRNKTEFDGVTSIVVD 83
Cdd:pfam05569   1 LFLLRASIAAAAAVLVVLLLRRPLRRYLGAIWAYALWLIVPLALLAPFIPEsaLSNFTTSAIFMARSKAPINSTAPKVID 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221   84 QSGVVLQHDFKTVFWMTVLDYLWVAWLVIFLLTFIYRIASYRNFKKYVFSGARRVDDLEQLDLLAETMEELNIKRPVELM 163
Cdd:pfam05569  81 DQASPLATPFPVVAWSTILKILLLLWIVGALILLLYMIAAYLVFRQRRVRRLGSLRAHELDDILKEAKEDMGIKRPITIS 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221  164 INPLISSPIFLGLKKNVIVIPDE---TFLNDELHYIFKHELVHCKRKDMYYVWMVQFFTCVYWFNPLMYLMNKRIQMDRE 240
Cdd:pfam05569 161 LSSNIDSPAVLGLWKPRIVLPADfdtRLSGEEIDYILAHELSHLKRGDLIINLLVAVLQCLHWFNPLVHLAFRKIRIDQE 240

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 489819221  241 LACDEAVLATLSKSKYIGYGDTLLSSLAKSGNYKESYVAVSLHENTKALKERLICI 296
Cdd:pfam05569 241 LACDAAVLARLHPHERKEYGRTLLKLLAGPSNHIVPVACVWLAGAKSALKERIMML 296
MecR1 COG4219
Signal transducer regulating beta-lactamase production, contains metallopeptidase domain ...
 109-326 4.13e-53

Signal transducer regulating beta-lactamase production, contains metallopeptidase domain [Signal transduction mechanisms];


Pssm-ID: 443363 [Multi-domain]  Cd Length: 337  Bit Score: 178.32  E-value: 4.13e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221 109 WLVIFLLTFIYRIASYRNFKKYVFSgARRVDDLEQLDLLAETMEELNIKRPVELMINPLISSPIFLGLKKNVIVIPDETF 188
Cdd:COG4219    1 WLAGVLLLLLRLLISLLRLRRLLRR-ARPVTDEELLELLERLARRLGIRRPVRLLESDRITSPFSFGLLRPVILLPAGLE 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221 189 L--NDELHYIFKHELVHCKRKDMYYVWMVQFFTCVYWFNPLMYLMNKRIQMDRELACDEAVLATLSKSKyiGYGDTLLSS 266
Cdd:COG4219   80 ElsEEELEAILAHELAHIRRRDLLDNLLAELLLALFWFNPLVWLARRRLRLDRELACDAAVLKAGGDRK--AYAETLLKL 157

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221 267 LAKSgnyKESYVAVSLHENTKALKERLICIANYETPSKKHNILFLIFIIVLCSVGVLFSA 326
Cdd:COG4219  158 AERR---SQPALALAFGGSKSTLKKRIKMLLKSKSKRRSRLKLLLALLLALLLALALLSA 214
M56_BlaR1_MecR1_like cd07341
Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; ...
 105-299 1.84e-44

Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; This family contains peptidase M56, which includes zinc metalloprotease domain in MecR1 as well as BlaR1. MecR1 is a transmembrane beta-lactam sensor/signal transducer protein that regulates the expression of an altered penicillin-binding protein PBP2a, which resists inactivation by beta-lactam antibiotics, in methicillin-resistant Staphylococcus aureus (MRSA). BlaR1 regulates the inducible expression of a class A beta-lactamase that hydrolytically destroys certain ?-lactam antibiotics in MRSA. Both, MecR1 and BlaR1, are transmembrane proteins that consist of four transmembrane helices, a cytoplasmic zinc protease domain, and the soluble C-terminal extracellular sensor domain, and are highly similar in sequence and function. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. All members contain the zinc metalloprotease motif (HEXXH). Homologs of this peptidase domain are also found in a number of other bacterial genome sequences, most of which are as yet uncharacterized.


Pssm-ID: 320700 [Multi-domain]  Cd Length: 187  Bit Score: 150.94  E-value: 1.84e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221 105 LWVAWLVIFLLTFIYRIASYRNFKKYvfsgARRVDDleqlDLLAETMEELNIKRPVELMINPLISSPIFLGLKKNVIVIP 184
Cdd:cd07341    1 IWLAGALLLLLRLLRGLLRLRRLRRR----AEPVPD----SLLLELARRLGLRRSVRLSVSALVASPMVVGLFRPVILLP 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221 185 DETFLN--DELHYIFKHELVHCKRKDMYYVWMVQFFTCVYWFNPLMYLMNKRIQMDRELACDEAVLATLSKSKyiGYGDT 262
Cdd:cd07341   73 EGLLEGspEELRAILLHELAHIRRRDLLVNLLQRLLEALFWFNPLVWLLSRRLRLERELACDEAVLAALGDKE--DYAEA 150

                        170       180       190
                 ....*....|....*....|....*....|....*..
gi 489819221 263 LLsSLAKSGNYKESYVAVSLHENTKALKERLICIANY 299
Cdd:cd07341  151 LL-RLAERRSQPPPALALALAGSKSLLKRRIKRILKK 186
M56_BlaR1_MecR1_like cd07326
Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; ...
 174-269 8.27e-08

Peptidase M56-like including those in BlaR1 and MecR1, integral membrane metallopeptidase; This family contains peptidase M56, which includes zinc metalloprotease domain in MecR1 as well as BlaR1. MecR1 is a transmembrane beta-lactam sensor/signal transducer protein that regulates the expression of an altered penicillin-binding protein PBP2a, which resists inactivation by beta-lactam antibiotics, in methicillin-resistant Staphylococcus aureus (MRSA). BlaR1 regulates the inducible expression of a class A beta-lactamase that hydrolytically destroys certain ?-lactam antibiotics in MRSA. Both, MecR1 and BlaR1, are transmembrane proteins that consist of four transmembrane helices, a cytoplasmic zinc protease domain, and the soluble C-terminal extracellular sensor domain, and are highly similar in sequence and function. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. All members contain the zinc metalloprotease motif (HEXXH). Homologs of this peptidase domain are also found in a number of other bacterial genome sequences, most of which are as yet uncharacterized.


Pssm-ID: 320685 [Multi-domain]  Cd Length: 165  Bit Score: 51.15  E-value: 8.27e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221 174 LGLKKNVIVIPD---ETFLNDELHYIFKHELVHCKRKDMYYVWMVQFFTCVYWFNPLMYLMNKRIQMDRELACDEAVLAT 250
Cdd:cd07326   42 LGGRRPRIVLSTgllELLSPEELRAVLAHERAHLRRRDPLLLLLASALARALPFLPLLRRLAAAYRLLRELAADDAAARR 121

                         90
                 ....*....|....*....
gi 489819221 251 LSKskyigygDTLLSSLAK 269
Cdd:cd07326  122 VGP-------RALASALLK 133
M56_like cd07329
Peptidase M56-like, integral membrane metallopeptidase in bacteria; This family contains ...
 145-269 1.51e-04

Peptidase M56-like, integral membrane metallopeptidase in bacteria; This family contains peptidase M56, which includes zinc metalloprotease domain in MecR1 as well as BlaR1. MecR1 is a transmembrane beta-lactam sensor/signal transducer protein that regulates the expression of an altered penicillin-binding protein PBP2a, which resists inactivation by beta-lactam antibiotics, in methicillin-resistant Staphylococcus aureus (MRSA). BlaR1 regulates the inducible expression of a class A beta-lactamase that hydrolytically destroys certain beta-lactam antibiotics in MRSA. Both, MecR1 and BlaR1, are transmembrane proteins that consist of four transmembrane helices, a cytoplasmic zinc protease domain, and the soluble C-terminal extracellular sensor domain, and are highly similar in sequence and function. The signal for protein expression is transmitted by site-specific proteolytic cleavage of both the transducer, which auto-activates, and the repressor, which is inactivated, unblocking gene transcription. All members contain the zinc metalloprotease motif (HEXXH). Homologs of this peptidase domain are also found in a number of other bacterial genome sequences, most of which are as yet uncharacterized.


Pssm-ID: 320688 [Multi-domain]  Cd Length: 188  Bit Score: 42.05  E-value: 1.51e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489819221 145 DLLAETMeelNIKRPVELMINPLISSPIFLGLKKN-VIVIPDETF--LN-DELHYIFKHELVHCKRKDMYYVWMVQ---- 216
Cdd:cd07329    1 DRLARQA---DVPPPRVYVVDSDVPNAFAVGRSRGpTVVVTTGLLdlLDdDELEAVLAHELAHLKRRDVLVLLLFDplll 77

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489819221 217 -------------FFTCVYWFNPLMYLMNKRIQMDRELACDEAVLATLSKSKY---IGYGDTLLSSLAK 269
Cdd:cd07329   78 lvvglllflslfiFELLGFFFQPLLFLAFFALLRLAELLADALAVARTSAARRarlTGLPAALASALEK 146
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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