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Conserved domains on  [gi|489965433|ref|WP_003868677|]
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MULTISPECIES: methylated-DNA--[protein]-cysteine S-methyltransferase [Thermoanaerobacter]

Protein Classification

methylated-DNA--[protein]-cysteine S-methyltransferase( domain architecture ID 11417447)

methylated-DNA--[protein]-cysteine S-methyltransferase is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA; it repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme

CATH:  3.30.160.70|1.10.10.10
EC:  2.1.1.63
Gene Ontology:  GO:0003908|GO:0003677|GO:0046872|GO:0006281|GO:0032259
PubMed:  17500045|17485252
SCOP:  4000565|4000678

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 1-155 4.87e-79

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


:

Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 231.30  E-value: 4.87e-79
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433   1 MLYKKFISPVGVLTIFSSGKGICRIDFENEKSS-----LEGFKEGSDFYIEECIRQLDLYFQKKLKNFDVRLDLQGTEFQ 75
Cdd:COG0350    2 IRYAIFDTPLGPLLIAATDRGLCALSFGDDREEallarFPAALREDPPLLAEAARQLDAYFAGERKDFDLPLDLRGTPFQ 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433  76 KAVWKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHEGVEV 155
Cdd:COG0350   82 RRVWEALRKIPYGETVTYGELARAIGRPKAARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLELEGALA 161
 
Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 1-155 4.87e-79

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 231.30  E-value: 4.87e-79
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433   1 MLYKKFISPVGVLTIFSSGKGICRIDFENEKSS-----LEGFKEGSDFYIEECIRQLDLYFQKKLKNFDVRLDLQGTEFQ 75
Cdd:COG0350    2 IRYAIFDTPLGPLLIAATDRGLCALSFGDDREEallarFPAALREDPPLLAEAARQLDAYFAGERKDFDLPLDLRGTPFQ 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433  76 KAVWKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHEGVEV 155
Cdd:COG0350   82 RRVWEALRKIPYGETVTYGELARAIGRPKAARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLELEGALA 161
PRK00901 PRK00901
methylated-DNA--protein-cysteine methyltransferase; Provisional
 8-152 1.13e-57

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 234860 [Multi-domain]  Cd Length: 155  Bit Score: 176.78  E-value: 1.13e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433   8 SPVGVLTIFSSGKGICRIDFENEKSSLEGFKEGSDFyIEECIRQLDLYFQKKLKNFDVRLDLQGTEFQKAVWKEILKIPY 87
Cdd:PRK00901   9 TPIGKIGIAENGTAITHLCFGEDKIPKDVTILETDL-LKEANKQLEEYFEGKRKKFDLPLAPQGTEFQKKVWKALQEIPY 87

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489965433  88 GEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHEG 152
Cdd:PRK00901  88 GETRSYKEIAVNIGNPKACRAVGLANNKNPIPIFIPCHRVIGANGKLVGYAGGLDIKEKLLKLEK 152
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
 73-153 2.04e-52

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 160.99  E-value: 2.04e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433   73 EFQKAVWKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHEG 152
Cdd:pfam01035   1 PFQRRVWEALRQIPYGKTTTYGEIAKLLGRPKAARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRALLELEG 80


                  .
gi 489965433  153 V 153
Cdd:pfam01035  81 V 81
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
 74-152 1.27e-47

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 148.78  E-value: 1.27e-47
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489965433  74 FQKAVWKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHEG 152
Cdd:cd06445    1 FQRRVWEALRQIPYGEVTTYGQIAKLAGTPKAARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELLELEG 79
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
 72-151 3.51e-47

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 147.85  E-value: 3.51e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433   72 TEFQKAVWKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHE 151
Cdd:TIGR00589   1 TPFQQKVWKALRTIPYGETKSYGQLAKAIGNPKAARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKEFLLEHE 80
 
Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 1-155 4.87e-79

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 231.30  E-value: 4.87e-79
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433   1 MLYKKFISPVGVLTIFSSGKGICRIDFENEKSS-----LEGFKEGSDFYIEECIRQLDLYFQKKLKNFDVRLDLQGTEFQ 75
Cdd:COG0350    2 IRYAIFDTPLGPLLIAATDRGLCALSFGDDREEallarFPAALREDPPLLAEAARQLDAYFAGERKDFDLPLDLRGTPFQ 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433  76 KAVWKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHEGVEV 155
Cdd:COG0350   82 RRVWEALRKIPYGETVTYGELARAIGRPKAARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLELEGALA 161
PRK00901 PRK00901
methylated-DNA--protein-cysteine methyltransferase; Provisional
 8-152 1.13e-57

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 234860 [Multi-domain]  Cd Length: 155  Bit Score: 176.78  E-value: 1.13e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433   8 SPVGVLTIFSSGKGICRIDFENEKSSLEGFKEGSDFyIEECIRQLDLYFQKKLKNFDVRLDLQGTEFQKAVWKEILKIPY 87
Cdd:PRK00901   9 TPIGKIGIAENGTAITHLCFGEDKIPKDVTILETDL-LKEANKQLEEYFEGKRKKFDLPLAPQGTEFQKKVWKALQEIPY 87

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 489965433  88 GEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHEG 152
Cdd:PRK00901  88 GETRSYKEIAVNIGNPKACRAVGLANNKNPIPIFIPCHRVIGANGKLVGYAGGLDIKEKLLKLEK 152
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
 73-153 2.04e-52

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 160.99  E-value: 2.04e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433   73 EFQKAVWKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHEG 152
Cdd:pfam01035   1 PFQRRVWEALRQIPYGKTTTYGEIAKLLGRPKAARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRALLELEG 80


                  .
gi 489965433  153 V 153
Cdd:pfam01035  81 V 81
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
 74-152 1.27e-47

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 148.78  E-value: 1.27e-47
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 489965433  74 FQKAVWKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHEG 152
Cdd:cd06445    1 FQRRVWEALRQIPYGEVTTYGQIAKLAGTPKAARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELLELEG 79
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
 72-151 3.51e-47

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 147.85  E-value: 3.51e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433   72 TEFQKAVWKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHE 151
Cdd:TIGR00589   1 TPFQQKVWKALRTIPYGETKSYGQLAKAIGNPKAARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKEFLLEHE 80
PRK10286 PRK10286
methylated-DNA--[protein]-cysteine S-methyltransferase;
71-152 1.47e-31

methylated-DNA--[protein]-cysteine S-methyltransferase;


Pssm-ID: 182355 [Multi-domain]  Cd Length: 171  Bit Score: 111.11  E-value: 1.47e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433  71 GTEFQKAVWKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRH 150
Cdd:PRK10286  86 GTPFQREVWQTLRTIPCGQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKEWLLRH 165


                 ..
gi 489965433 151 EG 152
Cdd:PRK10286 166 EG 167
AdaA COG2169
Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), ...
 8-151 2.08e-27

Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), contains Zn-binding and two AraC-type DNA-binding domains [Replication, recombination and repair];


Pssm-ID: 441772 [Multi-domain]  Cd Length: 358  Bit Score: 104.37  E-value: 2.08e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433   8 SPVGVLTIFSSGKGICRIDFENEKSSLE-----GFK----EGSDFYIEECIRQLDLYFQKKLKNFDVRLDLQGTEFQKAV 78
Cdd:COG2169  197 CSLGLLLVAASARGVCAILLGDDPEALLrdlqdRFPaaelIGGDAAFEQLVAEVVGFVEGPLLGLDLPLDLRGTAFQQRV 276

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 489965433  79 WKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHE 151
Cdd:COG2169  277 WQALRAIPAGETASYAEIAARIGAPKAVRAVAAACAANQLAVAIPCHRVVRADGALSGYRWGVERKRALLERE 349
Atl1 COG3695
Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like ...
 72-155 6.72e-27

Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like DNA-binding domain [Transcription];


Pssm-ID: 442910  Cd Length: 104  Bit Score: 96.79  E-value: 6.72e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433  72 TEFQKAVWKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLT-GFGGGIEIKKFLLRH 150
Cdd:COG3695    4 EEFYERVYEVVAQIPPGRVATYGDIAALAGLPRGARQVGRALRALPEGSDLPWHRVVNADGRLSpGHAGGAEEQRELLEA 83


                 ....*
gi 489965433 151 EGVEV 155
Cdd:COG3695   84 EGVPV 88
PRK15435 PRK15435
bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;
67-151 3.24e-23

bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;


Pssm-ID: 185333 [Multi-domain]  Cd Length: 353  Bit Score: 93.32  E-value: 3.24e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433  67 LDLQGTEFQKAVWKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKF 146
Cdd:PRK15435 264 LDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGALSGYRWGVSRKAQ 343


                 ....*
gi 489965433 147 LLRHE 151
Cdd:PRK15435 344 LLRRE 348
PRK03887 PRK03887
methylated-DNA--protein-cysteine methyltransferase; Provisional
 72-154 2.15e-16

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 167628 [Multi-domain]  Cd Length: 175  Bit Score: 72.07  E-value: 2.15e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489965433  72 TEFQKAVWKEILK-IPYGEVKTYGEIARLIGKpkAARAVGQALNKNPIPIIIPCHRVIGsSEDLTGFGGGIEIKKFLLRH 150
Cdd:PRK03887  91 TPFERKVYEWLTKnVKRGEVITYGELAKALNT--SPRAVGGAMKRNPYPIIVPCHRVVG-RKNPGLYTPKPEYKKFLLEV 167


                 ....
gi 489965433 151 EGVE 154
Cdd:PRK03887 168 EGVK 171
Methyltransf_1N pfam02870
6-O-methylguanine DNA methyltransferase, ribonuclease-like domain; This entry represents the ...
 1-37 1.81e-03

6-O-methylguanine DNA methyltransferase, ribonuclease-like domain; This entry represents the N-terminal ribonuclease-like domain associated with 6-O-methylguanine DNA methyltransferase activity. The repair of DNA containing O6-alkylated guanine is carried out by DNA-[protein]-cysteine S-methyltransferase (also known as O-6-methylguanine-DNA-alkyltransferase)


Pssm-ID: 397139 [Multi-domain]  Cd Length: 77  Bit Score: 35.42  E-value: 1.81e-03
                          10        20        30
                  ....*....|....*....|....*....|....*..
gi 489965433    1 MLYKKFISPVGVLTIFSSGKGICRIDFENEKSSLEGF 37
Cdd:pfam02870   2 LYYTLIDSPLGRLLLAGDERGLTAIDFLDKDYALRKE 38
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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