|
Name |
Accession |
Description |
Interval |
E-value |
| PFL1 |
cd01678 |
Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, ... |
11-756 |
0e+00 |
|
Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. The PFL mechanism involves an unusual radical cleavage of pyruvate in which two cysteines and one glycine form radicals that are required for catalysis. PFL has a ten-stranded alpha/beta barrel domain that is structurally similar to those of all three ribonucleotide reductase (RNR) classes as well as benzylsuccinate synthase and B12-independent glycerol dehydratase.
Pssm-ID: 153087 [Multi-domain] Cd Length: 738 Bit Score: 1462.20 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 11 AWKGFSEGEWQNNVNVRDFIQKNYTPYEGDESFLAGSTKATDTLWEQVMEGIKIENRTHAPVDFDTSVASTITSHDAGYI 90
Cdd:cd01678 1 AWEGFKGGKWQEEIDVRDFIQKNYTPYEGDESFLAGPTERTKKLWDKLEELLEEERAKGGVLDVDTKTVSTITSHKAGYI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 91 EKDLEQIVGLQTDAPLKRAIIPFGGIRMVESSCHAYNRELDPELKKIFTEYRKTHNQGVFDVYTPDILKCRKSGILTGLP 170
Cdd:cd01678 81 DKELEVIVGLQTDKPLKRAIMPFGGIRMAEQALKAYGYELDPELKKIFTKYRKTHNDGVFDAYTPEIRRARHSGIITGLP 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 171 DAYGRGRIIGDYRRVALYGIEFLRKDKFAQFTSLQEKlekgEDLEMTIQLREEIAEQHAALGQIQEMAAKYGCDISRPAQ 250
Cdd:cd01678 161 DAYGRGRIIGDYRRVALYGVDRLIEEKKKDLDNLGGD----EMTDDTIRLREEVAEQIKALKELKQMAASYGLDISRPAT 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 251 NAKEAVQWTYFGYLAAVKSQNGAAMSFGRVSTFLDIYIQRDLDAGLLTEEQAQELIDHLVMKLRMVRFLRTPEYDELFSG 330
Cdd:cd01678 237 NAQEAIQWTYFGYLAAIKEQNGAAMSLGRVSTFLDIYIERDLKAGTITEAEAQELIDQFIMKLRMVRFLRTPEYNELFSG 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 331 DPIWATESLGGMGLDGRTLVTKNTFRFLNTLYTMGPSPEPNMTILWSEQLPINFKKYAAKVSIDTSSIQYENDDLMRPDF 410
Cdd:cd01678 317 DPTWVTESIGGMGNDGRSLVTKTSFRFLNTLYNLGPAPEPNLTVLWSEKLPENFKRFCAKVSIDTSSIQYENDDLMRPDW 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 411 NSDDYAIACCVSPMVVGKQMQFFGARANLAKTLLYTINGGVDEKLKIQVGPKHAPIMDEVLDFDTVMNRMDQFMDWLATQ 490
Cdd:cd01678 397 GGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGRDEKTGDQVGPDIEPITSDYLDYDEVMENYDKSMDWLADT 476
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 491 YVTALNVIHYMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVSPIRDENGLAVDFNIEGEYPQFGNN 570
Cdd:cd01678 477 YVNALNIIHYMHDKYAYEALQMALHDTDVRRTMAFGIAGLSVAADSLSAIKYAKVKPIRDEDGLAVDFEIEGDFPRYGND 556
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 571 DSRVDDIACDLVERFMKKIQKLHTYRNAVPTQSILTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLT 650
Cdd:cd01678 557 DDRADDIAVWVVKTFMNKLRKHKTYRNAEPTQSVLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDKKGALASLA 636
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 651 SVAKLPFAYAKDGISYTFSIVPNALGKDDDVRKANLAGLMDGYFHHeadieGGQHLNVNVMNREMLLEAMEDPEKYPQLT 730
Cdd:cd01678 637 SVAKLPYRDANDGISNTFSIVPNALGKTDEERIDNLVGILDGYFTK-----GGHHLNVNVLNRETLLDAMEHPEKYPQLT 711
|
730 740
....*....|....*....|....*.
gi 490364911 731 IRVSGYAVRFNSLTKEQQQDVITRTF 756
Cdd:cd01678 712 IRVSGYAVNFVKLTREQQLDVISRTF 737
|
|
| pyr_form_ly_1 |
TIGR01255 |
formate acetyltransferase 1; Alternate names: pyruvate formate-lyase; formate ... |
11-760 |
0e+00 |
|
formate acetyltransferase 1; Alternate names: pyruvate formate-lyase; formate C-acetyltransferase This enzyme converts formate + acetyl-CoA into pyruvate + CoA. This model describes formate acetyltransferase 1. More distantly related putative formate acetyltransferases have also been identified, including formate acetyltransferase 2 from E. coli, which is excluded from this model. [Energy metabolism, Fermentation]
Pssm-ID: 273525 [Multi-domain] Cd Length: 744 Bit Score: 1339.95 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 11 AWKGFSEGEWQNNVNVRDFIQKNYTPYEGDESFLAGSTKATDTLWEQVMEgIKIENRTHAPVDFDTSVASTITSHDAGYI 90
Cdd:TIGR01255 1 AWEGFTKGDWQNEVNVRDFIQKNYKPYEGDESFLAGPTEATTKVWDKVME-VKLENRTHAPVDFDTAVASTITSHDAGYI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 91 EKDLEQIVGLQTDAPLKRAIIPFGGIRMVESSCHAYNRELDPELKKIFTEYRKTHNQGVFDVYTPDILKCRKSGILTGLP 170
Cdd:TIGR01255 80 DKQLEKIVGLQTEAPLKRALIPFGGIRMAEGSLKEYGRELDPMIHKIFTEYRKTHNQGVFDAYTPDIRRARKAGVLTGLP 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 171 DAYGRGRIIGDYRRVALYGIEFLRKDKFAQFTSLQEKLEkgedlEMTIQLREEIAEQHAALGQIQEMAAKYGCDISRPAQ 250
Cdd:TIGR01255 160 DAYGRGRIIGDYRRVALYGIDYLMKEKLKQFTSLQADLE-----NELIRLREEIAEQHRALGEMKEMAAKYGYDISRPAT 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 251 NAKEAVQWTYFGYLAAVKSQNGAAMSFGRVSTFLDIYIQRDLDAGLLTEEQAQELIDHLVMKLRMVRFLRTPEYDELFSG 330
Cdd:TIGR01255 235 NAKEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIERDLKAGKITEQEAQEMVDHFVMKLRMVRFLRTPEYDELFSG 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 331 DPIWATESLGGMGLDGRTLVTKNTFRFLNTLYTMGPSPEPNMTILWSEQLPINFKKYAAKVSIDTSSIQYENDDLMRPDF 410
Cdd:TIGR01255 315 DPTWATESIAGMGLDGRTLVTKNSFRFLNTLYTMGPAPEPNMTVLWSEKLPLSFKKFAAKMSIDTSSIQYENDDLMRPDF 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 411 NSDDYAIACCVSPMVVGKQMQFFGARANLAKTLLYTINGGVDEKLKIQVGPKHAPIMDEVLDFDTVMNRMDQFMDWLATQ 490
Cdd:TIGR01255 395 NNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVVPDIEPIKDEVLDYDEVMENMDKFLDWLAKQ 474
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 491 YVTALNVIHYMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVSPIRDENGLAVDFNIEGEYPQFGNN 570
Cdd:TIGR01255 475 YVTAMNIIHYMHDKYSYEASQMALHDTKVIRTMAFGIAGFSVAADSLSAIKYAKVKPIRDENGLAIDFEIEGDFPQYGND 554
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 571 DSRVDDIACDLVERFMKKIQKLHTYRNAVPTQSILTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLT 650
Cdd:TIGR01255 555 DDRVDDIAVDLVERFMKKLQKHHTYRNAIPTQSVLTITSNVVYGKKTGNTPDGRRVGAPFGPGANPMHGRDQKGALASLT 634
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 651 SVAKLPFAYAKDGISYTFSIVPNALGKDDDVRKANLAGLMDGYFHHEADIEGGQHLNVNVMNREMLLEAMEDPEKYPQLT 730
Cdd:TIGR01255 635 SVAKLPFAYAKDGISYTFSIVPNALGKDDDVRKTNLVGIMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLT 714
|
730 740 750
....*....|....*....|....*....|
gi 490364911 731 IRVSGYAVRFNSLTKEQQQDVITRTFTQTM 760
Cdd:TIGR01255 715 IRVSGYAVNFNSLTKEQQQEVITRTFHESL 744
|
|
| PflD |
COG1882 |
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ... |
41-756 |
0e+00 |
|
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation
Pssm-ID: 441486 [Multi-domain] Cd Length: 789 Bit Score: 1040.12 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 41 ESFLAGSTKATDTLWEQVMEG---IKIENRTH-----------APVDFDTSVASTITSHDAGYIeKDLEQIVGLQTDAPL 106
Cdd:COG1882 1 ESFLAGPTERTKRLREKLLEAkplIDIERARLftesyketeglPVIIRRAKAFSHILEHKPIYI-KDDELIVGLQTDKPR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 107 KRAIIPFGGIRMVESSCHAYNR------ELDPELKKIFTEY-----RKTHNQGVFDVYTPDILKCRKSGILTGLPDAYGR 175
Cdd:COG1882 80 KRPIFPEGGIRWVEDELDALPTrpqdgfEISPEDKEIFREIapywkGKTHNDGVFDAYPEEIRKARKAGIITGLPDAYGR 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 176 GRIIGDYRRVALYGIEFLRKDKFAQFTSLQ----EKLEKGEDLEM-------TIQLREEIAEQHAAL----------GQI 234
Cdd:COG1882 160 GHIIGDYRRVLLYGLDGLIEEAKEKLAELDltdpEDIEKIDFYKAmiivceaVIRLAERYAELARELaeketdpkrkAEL 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 235 QEMAAKYGCDISRPAQNAKEAVQWTYFGYLAAVKSQNGAAMSFGRVSTFLDIYIQRDLDAGLLTEEQAQELIDHLVMKLR 314
Cdd:COG1882 240 LEIAEICGFVPANPARTFWEAVQWVWFVYLAAIKEQNGAAMSLGRFDQYLYPYYERDLEEGRLTEEEAQELLDCFWIKLR 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 315 MVRFLRTPEYDELFSGDPIWATESLGGMGLDGRTLVTKNTFRFLNTLYTMgPSPEPNMTILWSEQLPINFKKYAAK-VSI 393
Cdd:COG1882 320 EVRFLRTPEYAELFAGYPTWVTLTIGGMTPDGRDAVNELSYLILETLRNL-PLPEPNLTVRWSEKLPEGFLKKAAEvISI 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 394 DTSSIQYENDDLMRPDF--------NSDDYAIACCVSPMVVGKQMQFFGA-RANLAKTLLYTINGGVDEKLKIQVGPKHA 464
Cdd:COG1882 399 GTGSPQYENDDLMIPMLlnkgvtleDARDYGIAGCVEPMVPGKQMQFFGAgRINLAKALEYALNNGVDEKTGKQVGPETG 478
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 465 PImDEVLDFDTVMNRMDQFMDWLATQYVTALNVIHYMHDKYSYEAALMALHDRDVYRTM------------ACGIAGLSV 532
Cdd:COG1882 479 DP-TDFLTYDEVMEAFKKQLDYLADLYVNALNIIHYMHDKYAPEPFLSALHDDCIERGKdlneggarynfgAIGIAGLSV 557
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 533 AADSLSAIK---YAKVSPIRDE--NGLAVDFniEG---------EYPQFGNNDSRVDDIACDLVERFMKKIQKLHTYRNA 598
Cdd:COG1882 558 VADSLSAIKklvFDKKKVTMDEllEALAANF--EGyeelrqlllNAPKYGNDDDYVDEIAVELVETFMDEIRKYKTYRGG 635
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 599 VPTQSILTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKD 678
Cdd:COG1882 636 TYTLSILTITSNVPYGKKTGATPDGRKAGEPLADGASPMHGRDKNGPTAVLKSVAKLPYEKATDGILLNQKFSPSALGGE 715
|
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 490364911 679 DDVRkaNLAGLMDGYFHHeadieGGQHLNVNVMNREMLLEAMEDPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTF 756
Cdd:COG1882 716 EGIE--NLVSLLRTYFDL-----GGHHIQFNVVDRETLLDAQKHPEKYPDLTVRVAGYSAYFVELSKEQQDDIIARTE 786
|
|
| PFL-like |
pfam02901 |
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ... |
30-614 |
0e+00 |
|
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.
Pssm-ID: 427048 Cd Length: 647 Bit Score: 753.31 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 30 IQKNYTPYEGDESFLagstkatdtlWEQVMEGIKIENRTHAPVDFDTSVAST---ITSHDAGYIeKDLEQIVGLQTDAPL 106
Cdd:pfam02901 8 LKENYTLYTGDPSLS----------WERARLLTESYKETEGVLPVDIRRAKAlkkILSHLPGYI-RDDELIVGLQTDKPR 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 107 KRAIIPFGGIRMVESSCHAYNR------ELDPELKKIFTE-----YRKTHNQGVFDVYTPDILKCRKSGILTGLPDAYGR 175
Cdd:pfam02901 77 KRAIYPEGGIRWVEDELDYLNTrpqdgfEISEEDKKIFREifpywKGKTHNEGVFDAYTPEMKAARESGIFTGLPDAYGR 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 176 GRIIGDYRRVALYGIEFLRKDKFAQFTSL---QEKLEKGEDL--------------EMTIQLREEIAEQHA---ALGQIQ 235
Cdd:pfam02901 157 GHIIGDYRRVLLYGLDGLIEEKEEKLAKLdtdPEDIEKIEFYkamiiscdavieyaERYARLAEELAEQETdpkRKAELL 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 236 EMAAKYGCDISRPAQNAKEAVQWTYFGYLAAVKSQNGAAMSFGRVSTFLDIYIQRDLDAGLLTEEQAQELIDHLVMKLRM 315
Cdd:pfam02901 237 EIAEICGRVPARPAETFQEAIQWFWFVYLAAVKEQNGAAMSLGRLDQYLYPYYERDLEEGRLTEEEAQELIDCFWIKLRE 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 316 VRFLRTPEYDELFSGDPIWATESLGGMGLDGRTLVTKNTFRFLNTLYTMgPSPEPNMTILWSEQLPINFKKYAAKVSID- 394
Cdd:pfam02901 317 VRFLRTPEYNKLFAGYDPFQNLTIGGQGRDGRDAVNKLSYLILEALDNL-PLPEPNLTVRWSKKLPEEFLKKAAEVSRKg 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 395 TSSIQYENDDLMRPDFNSD--------DYAIACCVSPMVVGKQMQFFGARANLAKTLLYTINGGVDEKLKIQVGPKHAPI 466
Cdd:pfam02901 396 TGSPQYENDDVMIPALLNRgvsledarDYGIAGCVEPMKPGKEMQFFGARINLAKALEYALNGGRDELTGKQVGPKTGPV 475
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 467 mDEVLDFDTVMNRMDQFMDWLATQYVTALNVIHYMHDKYSYEAALMALHDRDVYRTMACGI------------AGLSVAA 534
Cdd:pfam02901 476 -TEFLSFEEVMEAFKKQLDYLADLYVNALNIIHYMHDKYAPEPFLSALHDDCIERGKDVGIggarynfsgpqgAGLANVA 554
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 535 DSLSAIKY----AKVSPIRD-ENGLAVDFNIEGE--------YPQFGNNDSRVDDIACDLVERFMKKIQKLHTYRNAVPT 601
Cdd:pfam02901 555 DSLSAIKKlvfdDKVYTLRElEDALAADFEGEEElrqdllndAPKYGNDDDRVDDIAVEVVETFMDEVRKYKNYRGGKFT 634
|
650
....*....|...
gi 490364911 602 QSILTITSNVVYG 614
Cdd:pfam02901 635 PSLLTITSNVPYG 647
|
|
| pflD |
PRK09983 |
putative formate acetyltransferase 2; Provisional |
174-758 |
6.20e-41 |
|
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain] Cd Length: 765 Bit Score: 160.76 E-value: 6.20e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 174 GRGRIIGDYRRVALYG----IEFLRK-------DKFAQFTSLQEKLEKGEDLEMTiQLREEIAEQHAALGQIQEM---AA 239
Cdd:PRK09983 153 GQGHIIIDYPRLLNHGlgelVAQMQQhcqqqpeNHFYQAALLLLEASQKHILRYA-ELAETMAANCTDAQRREELltiAE 231
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 240 KYGCDISRPAQNAKEAVQWTYFGYLAAVKSQNGAAMSFGRVSTFLDIYIQRDLDAGLlTEEQAQELIDHLVMKLRMVRFL 319
Cdd:PRK09983 232 ISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGE-DPAFLKELLESLWVKCNDIVLL 310
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 320 RTPEYDELFSGDPIWATESLGGMGLDGRTLVTKNTFRFLNTlYTMGPSPEPNMTILWSEQLPINF-KKYAAKVSIDTSSI 398
Cdd:PRK09983 311 RSTSSARYFAGFPTGYTALLGGLTENGRSAVNVLSFLCLDA-YQSVQLPQPNLGVRTNALIDTPFlMKTAETIRLGTGIP 389
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 399 QYENDDLMRPDF--------NSDDYAIACCVSPMVVGKQmqfFG----ARANLAKTLLYTI--NGGVDEklkiqvgpkha 464
Cdd:PRK09983 390 QIFNDEVVVPAFlnrgvsleDARDYSVVGCVELSIPGRT---YGlhdiAMFNLLKVMEICLheNEGNAA----------- 455
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 465 pimdevLDFDTVMNRMDQFMDWLATQYVTALNVIHYMHDKYSYEAALMAL------HDRDVY----RTMACGIAGLSVA- 533
Cdd:PRK09983 456 ------LTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFisdcleKGRDITdggaRYNFSGVQGIGIAn 529
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 534 -ADSLSAIKyakvSPIRDENGLAVD---------FNI-EGE---------YPQFGNNDSRVDDIACDLVERFMKKIQKLH 593
Cdd:PRK09983 530 lSDSLHALK----GMVFDQQRLSFDellsvlkanFATpEGEkvrarlinrFEKYGNDIDEVDNISAELLRHYCKEVEKYQ 605
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 594 TYRNAVPTQSILTITSNVVYGKKTGNTPDGRRAGAPFGPGA-NPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVP 672
Cdd:PRK09983 606 NPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGGlSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTP 685
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 673 NALGKDDDVRKanLAGLMDGYFHHEAdieggQHLNVNVMNREMLLEAMEDPEKYPQLTIRVSGYAVRFNSLTKEQQQDVI 752
Cdd:PRK09983 686 ATLEGEAGLRK--LADFLRAFTQLKL-----QHIQFNVVNADTLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDII 758
|
....*.
gi 490364911 753 TRTFTQ 758
Cdd:PRK09983 759 RRTAHQ 764
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| PFL1 |
cd01678 |
Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, ... |
11-756 |
0e+00 |
|
Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. The PFL mechanism involves an unusual radical cleavage of pyruvate in which two cysteines and one glycine form radicals that are required for catalysis. PFL has a ten-stranded alpha/beta barrel domain that is structurally similar to those of all three ribonucleotide reductase (RNR) classes as well as benzylsuccinate synthase and B12-independent glycerol dehydratase.
Pssm-ID: 153087 [Multi-domain] Cd Length: 738 Bit Score: 1462.20 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 11 AWKGFSEGEWQNNVNVRDFIQKNYTPYEGDESFLAGSTKATDTLWEQVMEGIKIENRTHAPVDFDTSVASTITSHDAGYI 90
Cdd:cd01678 1 AWEGFKGGKWQEEIDVRDFIQKNYTPYEGDESFLAGPTERTKKLWDKLEELLEEERAKGGVLDVDTKTVSTITSHKAGYI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 91 EKDLEQIVGLQTDAPLKRAIIPFGGIRMVESSCHAYNRELDPELKKIFTEYRKTHNQGVFDVYTPDILKCRKSGILTGLP 170
Cdd:cd01678 81 DKELEVIVGLQTDKPLKRAIMPFGGIRMAEQALKAYGYELDPELKKIFTKYRKTHNDGVFDAYTPEIRRARHSGIITGLP 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 171 DAYGRGRIIGDYRRVALYGIEFLRKDKFAQFTSLQEKlekgEDLEMTIQLREEIAEQHAALGQIQEMAAKYGCDISRPAQ 250
Cdd:cd01678 161 DAYGRGRIIGDYRRVALYGVDRLIEEKKKDLDNLGGD----EMTDDTIRLREEVAEQIKALKELKQMAASYGLDISRPAT 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 251 NAKEAVQWTYFGYLAAVKSQNGAAMSFGRVSTFLDIYIQRDLDAGLLTEEQAQELIDHLVMKLRMVRFLRTPEYDELFSG 330
Cdd:cd01678 237 NAQEAIQWTYFGYLAAIKEQNGAAMSLGRVSTFLDIYIERDLKAGTITEAEAQELIDQFIMKLRMVRFLRTPEYNELFSG 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 331 DPIWATESLGGMGLDGRTLVTKNTFRFLNTLYTMGPSPEPNMTILWSEQLPINFKKYAAKVSIDTSSIQYENDDLMRPDF 410
Cdd:cd01678 317 DPTWVTESIGGMGNDGRSLVTKTSFRFLNTLYNLGPAPEPNLTVLWSEKLPENFKRFCAKVSIDTSSIQYENDDLMRPDW 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 411 NSDDYAIACCVSPMVVGKQMQFFGARANLAKTLLYTINGGVDEKLKIQVGPKHAPIMDEVLDFDTVMNRMDQFMDWLATQ 490
Cdd:cd01678 397 GGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGRDEKTGDQVGPDIEPITSDYLDYDEVMENYDKSMDWLADT 476
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 491 YVTALNVIHYMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVSPIRDENGLAVDFNIEGEYPQFGNN 570
Cdd:cd01678 477 YVNALNIIHYMHDKYAYEALQMALHDTDVRRTMAFGIAGLSVAADSLSAIKYAKVKPIRDEDGLAVDFEIEGDFPRYGND 556
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 571 DSRVDDIACDLVERFMKKIQKLHTYRNAVPTQSILTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLT 650
Cdd:cd01678 557 DDRADDIAVWVVKTFMNKLRKHKTYRNAEPTQSVLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDKKGALASLA 636
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 651 SVAKLPFAYAKDGISYTFSIVPNALGKDDDVRKANLAGLMDGYFHHeadieGGQHLNVNVMNREMLLEAMEDPEKYPQLT 730
Cdd:cd01678 637 SVAKLPYRDANDGISNTFSIVPNALGKTDEERIDNLVGILDGYFTK-----GGHHLNVNVLNRETLLDAMEHPEKYPQLT 711
|
730 740
....*....|....*....|....*.
gi 490364911 731 IRVSGYAVRFNSLTKEQQQDVITRTF 756
Cdd:cd01678 712 IRVSGYAVNFVKLTREQQLDVISRTF 737
|
|
| pyr_form_ly_1 |
TIGR01255 |
formate acetyltransferase 1; Alternate names: pyruvate formate-lyase; formate ... |
11-760 |
0e+00 |
|
formate acetyltransferase 1; Alternate names: pyruvate formate-lyase; formate C-acetyltransferase This enzyme converts formate + acetyl-CoA into pyruvate + CoA. This model describes formate acetyltransferase 1. More distantly related putative formate acetyltransferases have also been identified, including formate acetyltransferase 2 from E. coli, which is excluded from this model. [Energy metabolism, Fermentation]
Pssm-ID: 273525 [Multi-domain] Cd Length: 744 Bit Score: 1339.95 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 11 AWKGFSEGEWQNNVNVRDFIQKNYTPYEGDESFLAGSTKATDTLWEQVMEgIKIENRTHAPVDFDTSVASTITSHDAGYI 90
Cdd:TIGR01255 1 AWEGFTKGDWQNEVNVRDFIQKNYKPYEGDESFLAGPTEATTKVWDKVME-VKLENRTHAPVDFDTAVASTITSHDAGYI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 91 EKDLEQIVGLQTDAPLKRAIIPFGGIRMVESSCHAYNRELDPELKKIFTEYRKTHNQGVFDVYTPDILKCRKSGILTGLP 170
Cdd:TIGR01255 80 DKQLEKIVGLQTEAPLKRALIPFGGIRMAEGSLKEYGRELDPMIHKIFTEYRKTHNQGVFDAYTPDIRRARKAGVLTGLP 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 171 DAYGRGRIIGDYRRVALYGIEFLRKDKFAQFTSLQEKLEkgedlEMTIQLREEIAEQHAALGQIQEMAAKYGCDISRPAQ 250
Cdd:TIGR01255 160 DAYGRGRIIGDYRRVALYGIDYLMKEKLKQFTSLQADLE-----NELIRLREEIAEQHRALGEMKEMAAKYGYDISRPAT 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 251 NAKEAVQWTYFGYLAAVKSQNGAAMSFGRVSTFLDIYIQRDLDAGLLTEEQAQELIDHLVMKLRMVRFLRTPEYDELFSG 330
Cdd:TIGR01255 235 NAKEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIERDLKAGKITEQEAQEMVDHFVMKLRMVRFLRTPEYDELFSG 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 331 DPIWATESLGGMGLDGRTLVTKNTFRFLNTLYTMGPSPEPNMTILWSEQLPINFKKYAAKVSIDTSSIQYENDDLMRPDF 410
Cdd:TIGR01255 315 DPTWATESIAGMGLDGRTLVTKNSFRFLNTLYTMGPAPEPNMTVLWSEKLPLSFKKFAAKMSIDTSSIQYENDDLMRPDF 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 411 NSDDYAIACCVSPMVVGKQMQFFGARANLAKTLLYTINGGVDEKLKIQVGPKHAPIMDEVLDFDTVMNRMDQFMDWLATQ 490
Cdd:TIGR01255 395 NNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVVPDIEPIKDEVLDYDEVMENMDKFLDWLAKQ 474
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 491 YVTALNVIHYMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVSPIRDENGLAVDFNIEGEYPQFGNN 570
Cdd:TIGR01255 475 YVTAMNIIHYMHDKYSYEASQMALHDTKVIRTMAFGIAGFSVAADSLSAIKYAKVKPIRDENGLAIDFEIEGDFPQYGND 554
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 571 DSRVDDIACDLVERFMKKIQKLHTYRNAVPTQSILTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLT 650
Cdd:TIGR01255 555 DDRVDDIAVDLVERFMKKLQKHHTYRNAIPTQSVLTITSNVVYGKKTGNTPDGRRVGAPFGPGANPMHGRDQKGALASLT 634
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 651 SVAKLPFAYAKDGISYTFSIVPNALGKDDDVRKANLAGLMDGYFHHEADIEGGQHLNVNVMNREMLLEAMEDPEKYPQLT 730
Cdd:TIGR01255 635 SVAKLPFAYAKDGISYTFSIVPNALGKDDDVRKTNLVGIMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLT 714
|
730 740 750
....*....|....*....|....*....|
gi 490364911 731 IRVSGYAVRFNSLTKEQQQDVITRTFTQTM 760
Cdd:TIGR01255 715 IRVSGYAVNFNSLTKEQQQEVITRTFHESL 744
|
|
| PflD |
COG1882 |
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ... |
41-756 |
0e+00 |
|
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation
Pssm-ID: 441486 [Multi-domain] Cd Length: 789 Bit Score: 1040.12 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 41 ESFLAGSTKATDTLWEQVMEG---IKIENRTH-----------APVDFDTSVASTITSHDAGYIeKDLEQIVGLQTDAPL 106
Cdd:COG1882 1 ESFLAGPTERTKRLREKLLEAkplIDIERARLftesyketeglPVIIRRAKAFSHILEHKPIYI-KDDELIVGLQTDKPR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 107 KRAIIPFGGIRMVESSCHAYNR------ELDPELKKIFTEY-----RKTHNQGVFDVYTPDILKCRKSGILTGLPDAYGR 175
Cdd:COG1882 80 KRPIFPEGGIRWVEDELDALPTrpqdgfEISPEDKEIFREIapywkGKTHNDGVFDAYPEEIRKARKAGIITGLPDAYGR 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 176 GRIIGDYRRVALYGIEFLRKDKFAQFTSLQ----EKLEKGEDLEM-------TIQLREEIAEQHAAL----------GQI 234
Cdd:COG1882 160 GHIIGDYRRVLLYGLDGLIEEAKEKLAELDltdpEDIEKIDFYKAmiivceaVIRLAERYAELARELaeketdpkrkAEL 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 235 QEMAAKYGCDISRPAQNAKEAVQWTYFGYLAAVKSQNGAAMSFGRVSTFLDIYIQRDLDAGLLTEEQAQELIDHLVMKLR 314
Cdd:COG1882 240 LEIAEICGFVPANPARTFWEAVQWVWFVYLAAIKEQNGAAMSLGRFDQYLYPYYERDLEEGRLTEEEAQELLDCFWIKLR 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 315 MVRFLRTPEYDELFSGDPIWATESLGGMGLDGRTLVTKNTFRFLNTLYTMgPSPEPNMTILWSEQLPINFKKYAAK-VSI 393
Cdd:COG1882 320 EVRFLRTPEYAELFAGYPTWVTLTIGGMTPDGRDAVNELSYLILETLRNL-PLPEPNLTVRWSEKLPEGFLKKAAEvISI 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 394 DTSSIQYENDDLMRPDF--------NSDDYAIACCVSPMVVGKQMQFFGA-RANLAKTLLYTINGGVDEKLKIQVGPKHA 464
Cdd:COG1882 399 GTGSPQYENDDLMIPMLlnkgvtleDARDYGIAGCVEPMVPGKQMQFFGAgRINLAKALEYALNNGVDEKTGKQVGPETG 478
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 465 PImDEVLDFDTVMNRMDQFMDWLATQYVTALNVIHYMHDKYSYEAALMALHDRDVYRTM------------ACGIAGLSV 532
Cdd:COG1882 479 DP-TDFLTYDEVMEAFKKQLDYLADLYVNALNIIHYMHDKYAPEPFLSALHDDCIERGKdlneggarynfgAIGIAGLSV 557
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 533 AADSLSAIK---YAKVSPIRDE--NGLAVDFniEG---------EYPQFGNNDSRVDDIACDLVERFMKKIQKLHTYRNA 598
Cdd:COG1882 558 VADSLSAIKklvFDKKKVTMDEllEALAANF--EGyeelrqlllNAPKYGNDDDYVDEIAVELVETFMDEIRKYKTYRGG 635
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 599 VPTQSILTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKD 678
Cdd:COG1882 636 TYTLSILTITSNVPYGKKTGATPDGRKAGEPLADGASPMHGRDKNGPTAVLKSVAKLPYEKATDGILLNQKFSPSALGGE 715
|
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 490364911 679 DDVRkaNLAGLMDGYFHHeadieGGQHLNVNVMNREMLLEAMEDPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTF 756
Cdd:COG1882 716 EGIE--NLVSLLRTYFDL-----GGHHIQFNVVDRETLLDAQKHPEKYPDLTVRVAGYSAYFVELSKEQQDDIIARTE 786
|
|
| PFL-like |
pfam02901 |
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ... |
30-614 |
0e+00 |
|
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.
Pssm-ID: 427048 Cd Length: 647 Bit Score: 753.31 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 30 IQKNYTPYEGDESFLagstkatdtlWEQVMEGIKIENRTHAPVDFDTSVAST---ITSHDAGYIeKDLEQIVGLQTDAPL 106
Cdd:pfam02901 8 LKENYTLYTGDPSLS----------WERARLLTESYKETEGVLPVDIRRAKAlkkILSHLPGYI-RDDELIVGLQTDKPR 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 107 KRAIIPFGGIRMVESSCHAYNR------ELDPELKKIFTE-----YRKTHNQGVFDVYTPDILKCRKSGILTGLPDAYGR 175
Cdd:pfam02901 77 KRAIYPEGGIRWVEDELDYLNTrpqdgfEISEEDKKIFREifpywKGKTHNEGVFDAYTPEMKAARESGIFTGLPDAYGR 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 176 GRIIGDYRRVALYGIEFLRKDKFAQFTSL---QEKLEKGEDL--------------EMTIQLREEIAEQHA---ALGQIQ 235
Cdd:pfam02901 157 GHIIGDYRRVLLYGLDGLIEEKEEKLAKLdtdPEDIEKIEFYkamiiscdavieyaERYARLAEELAEQETdpkRKAELL 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 236 EMAAKYGCDISRPAQNAKEAVQWTYFGYLAAVKSQNGAAMSFGRVSTFLDIYIQRDLDAGLLTEEQAQELIDHLVMKLRM 315
Cdd:pfam02901 237 EIAEICGRVPARPAETFQEAIQWFWFVYLAAVKEQNGAAMSLGRLDQYLYPYYERDLEEGRLTEEEAQELIDCFWIKLRE 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 316 VRFLRTPEYDELFSGDPIWATESLGGMGLDGRTLVTKNTFRFLNTLYTMgPSPEPNMTILWSEQLPINFKKYAAKVSID- 394
Cdd:pfam02901 317 VRFLRTPEYNKLFAGYDPFQNLTIGGQGRDGRDAVNKLSYLILEALDNL-PLPEPNLTVRWSKKLPEEFLKKAAEVSRKg 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 395 TSSIQYENDDLMRPDFNSD--------DYAIACCVSPMVVGKQMQFFGARANLAKTLLYTINGGVDEKLKIQVGPKHAPI 466
Cdd:pfam02901 396 TGSPQYENDDVMIPALLNRgvsledarDYGIAGCVEPMKPGKEMQFFGARINLAKALEYALNGGRDELTGKQVGPKTGPV 475
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 467 mDEVLDFDTVMNRMDQFMDWLATQYVTALNVIHYMHDKYSYEAALMALHDRDVYRTMACGI------------AGLSVAA 534
Cdd:pfam02901 476 -TEFLSFEEVMEAFKKQLDYLADLYVNALNIIHYMHDKYAPEPFLSALHDDCIERGKDVGIggarynfsgpqgAGLANVA 554
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 535 DSLSAIKY----AKVSPIRD-ENGLAVDFNIEGE--------YPQFGNNDSRVDDIACDLVERFMKKIQKLHTYRNAVPT 601
Cdd:pfam02901 555 DSLSAIKKlvfdDKVYTLRElEDALAADFEGEEElrqdllndAPKYGNDDDRVDDIAVEVVETFMDEVRKYKNYRGGKFT 634
|
650
....*....|...
gi 490364911 602 QSILTITSNVVYG 614
Cdd:pfam02901 635 PSLLTITSNVPYG 647
|
|
| PFL2_DhaB_BssA |
cd01677 |
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ... |
89-755 |
2.39e-85 |
|
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.
Pssm-ID: 153086 [Multi-domain] Cd Length: 781 Bit Score: 286.87 E-value: 2.39e-85
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 89 YIEKDlEQIVGlqtdaplKRAIIPFGGIRMVESSCHA--------YNRELDP----ELKKIFTE------YRKTHNQGVF 150
Cdd:cd01677 56 YIQDD-ELIVG-------NRGGKPRAVPVFPELSVHWvedelddlPKRPGDPfvisEDKKEYLEeifpywKGKTLRDRCF 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 151 DVYTPDILKCRKSGILTGLPDAY-GRGRIIGDYRRVALYGIEFLRKDKFAQFTSLQ----EKLEKGEDLEMTIQLREEI- 224
Cdd:cd01677 128 KYFPEETLIAMAAGVFTEFMYFFsGPGHVAVDYPKVLEKGLDGLIEEAKEAIEALDltgpEDIDKIYFYQAMIIVCEAVi 207
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 225 --AEQHAALGQiqEMAAKYG-----------CDISR-----PAQNAKEAVQWTYFGYLAAVKSQNGAAMSFGRVSTFLDI 286
Cdd:cd01677 208 tyAKRYAELAK--ELAAKETdpkrkaelleiAEICRrvpahPPRTFWEALQSFWFIHLILQIESNGHSISPGRFDQYLYP 285
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 287 YIQRDLDAGLLTEEQAQELIDHLVMKLRMVRFLRTPEYDELFSGDPIWATESLGGMGLDGRTLVTKNTFRFLNTLYTMgP 366
Cdd:cd01677 286 FYKQDIEEGRLTREGAIELLECLWIKINEINKVRSGASAKYFAGYNTFQNLTIGGQTEDGSDATNELSYLILEATRRV-R 364
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 367 SPEPNMTILWSEQLPINFKKYAAKVSIDTSSI-QYENDDLMRPDF--------NSDDYAIACCVSPMVVGKQMQFFGARA 437
Cdd:cd01677 365 LPQPSLTVRYHAKSPDKFLKKAAEVIRLGLGYpAFFNDEVVIPALlrkgvsleDARDYGLIGCVETGAPGRKYRWTGTGY 444
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 438 -NLAKTLLYTINGGVDEKLKIQVGPkhapIMDEVLDFDTvmnrMDQFMDWLATQY-------VTALNVIHYMHDKYSYEA 509
Cdd:cd01677 445 iNLAKVLEITLNNGKDPRSGKQVGP----ETGDATDFKT----FEELWEAFKKQLrhfiklsVRANNISDIAHAEVAPAP 516
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 510 ALMALHD------RDVYRTMA---------CGIAglsVAADSLSAIKYAkvspIRDENGL-------AVDFNIEGEY--- 564
Cdd:cd01677 517 FLSALVDdciekgKDINAGGArynfggiqgVGIA---TLGDSLAAIKKL----VFEEKKLtmeelleALKANFAEGYeer 589
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 565 -------PQFGNNDSRVDDIACDLVERFMKKIQKLHTYRNAVPTQSILTITSNVVYGKKTGNTPDGRRAGAPFGPGANPM 637
Cdd:cd01677 590 rrllnnaPKYGNDDDYADNIARRVYEWYCKEVEKYQNPRGGKFYPGTYSVSANVPFGSVTGATPDGRLAGTPLSDGVSPS 669
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 638 HGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDDVRKanLAGLMDGYFhheadIEGGQHLNVNVMNREMLL 717
Cdd:cd01677 670 QGTDKKGPTAVIKSVSKLDHFNISGGTLLNQKFSPSTLEGEEGLKK--LAALIRTYF-----DLGGHHIQFNVVSAETLR 742
|
730 740 750
....*....|....*....|....*....|....*...
gi 490364911 718 EAMEDPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRT 755
Cdd:cd01677 743 DAQKHPEKYRDLIVRVAGYSAYFVELSKEVQDEIIART 780
|
|
| RNR_PFL |
cd00576 |
Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and ... |
154-710 |
8.87e-84 |
|
Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and pyruvate formate lyase (PFL) are believed to have diverged from a common ancestor. They have a structurally similar ten-stranded alpha-beta barrel domain that hosts the active site, and are radical enzymes. RNRs are found in all organisms and provide the only mechanism by which nucleotides are converted to deoxynucleotides. RNRs are separated into three classes based on their metallocofactor usage. Class I RNRs use a diiron-tyrosyl radical while Class II RNRs use coenzyme B12 (adenosylcobalamin, AdoCbl). Class III RNRs use an FeS cluster and S-adenosylmethionine to generate a glycyl radical. PFL, an essential enzyme in anaerobic bacteria, catalyzes the conversion of pyruvate and CoA to acteylCoA and formate in a mechanism that uses a glycyl radical.
Pssm-ID: 153083 [Multi-domain] Cd Length: 401 Bit Score: 271.71 E-value: 8.87e-84
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 154 TPDILKCRKSGILTGLPDAY-GRGRIIGDYRRVALYGIEFlrkdkfaqftslqeklekgedlemtiqlreeiaeqhaalg 232
Cdd:cd00576 1 AERIYEAVKSGVITVGRPDLpFTGCVLVDYGDSLDPGIKG---------------------------------------- 40
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 233 qiqemaakygcdISRPAQNAKEAVQWTYFGYLAAVKSQNGAAMSFGRVSTFLDIYIQRDLDAGLLTEEQAQELIDHLVMK 312
Cdd:cd00576 41 ------------VNETAKSINEAIQKTYQIIALAASNQNGGGVSFARASSILSPYGSRDYAKGSGTETDAVEAADAFNLA 108
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 313 LRMVRFLrtpeydelfSGDPIWATESLGGMGLDgrtLVTKNTFRFLNTLYTMG----PSPEPNMTILWSEQLP------I 382
Cdd:cd00576 109 LKEVGQG---------NGRTGAATGFIGGVHKG---KGDKISQEFLNLALANGgegiPLNFPNLSVRVSSDKPgilvkaV 176
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 383 NFKKYAAKVSIDTSSIQYENDdlmrpdfnsddyaIACcvspmvvgkqmqfFGARANLAKTLLYTINGgvdeklkiqvgpk 462
Cdd:cd00576 177 ELKQLIAEEARKTGSPGIFND-------------ELC-------------NLVSLNLARIMEKAING------------- 217
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 463 hapimdevldfdtVMNRMDQFMDWLATQYVTALNVIHYMHDKYSYEaalmALHDRDVYRTMACGIAGLSVAADSLSAIKY 542
Cdd:cd00576 218 -------------SMDVVLEELEELAFLAVRALDCVIDSHDERIPT----IELGGDERRTVGLGIAGVADLLIKLGLEKV 280
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 543 AkvspirdenglavdfniegeypqfgnnDSRVDDIACDLVERFMKKIQKLHTYRNAVPTQSILTITSNvvygkktgntpd 622
Cdd:cd00576 281 G---------------------------DPEADDLAAELVDQLKKHLVKATNERGFNFLLGLSPSESN------------ 321
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 623 grRAGAPFGPGANPMHGR------DQKGAVASLTSVAKLPFaYAKDGISYTFSIVPNALGKDddvrkaNLAGLMDGYFHH 696
Cdd:cd00576 322 --SSGAPATNGVSPSRG*iaivlnGDIGPEESLASVAILQF-YADNGISDTITIPDSATNLD------QLLAVIDGAAAI 392
|
570
....*....|....
gi 490364911 697 eadieGGQHLNVNV 710
Cdd:cd00576 393 -----KTTHVRVNP 401
|
|
| Gly_radical |
pfam01228 |
Glycine radical; |
630-741 |
2.29e-49 |
|
Glycine radical;
Pssm-ID: 426140 [Multi-domain] Cd Length: 106 Bit Score: 168.50 E-value: 2.29e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 630 FGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDDVRKANLAGLMDGYFHheadieGGQHLNVN 709
Cdd:pfam01228 1 VAPGISPSHGADFEGPTAVLNSVGKIDYEVELDGISLNQKFLPAVLGYYDEEGYANLNTLIDTYFE------GGHHLQFN 74
|
90 100 110
....*....|....*....|....*....|..
gi 490364911 710 VMNREMLLEAMEDPEKYPQLTIRVSGYAVRFN 741
Cdd:pfam01228 75 VVDRETLPDAQKHPEKYPDLTVRVSGYSANFV 106
|
|
| pflD |
PRK09983 |
putative formate acetyltransferase 2; Provisional |
174-758 |
6.20e-41 |
|
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain] Cd Length: 765 Bit Score: 160.76 E-value: 6.20e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 174 GRGRIIGDYRRVALYG----IEFLRK-------DKFAQFTSLQEKLEKGEDLEMTiQLREEIAEQHAALGQIQEM---AA 239
Cdd:PRK09983 153 GQGHIIIDYPRLLNHGlgelVAQMQQhcqqqpeNHFYQAALLLLEASQKHILRYA-ELAETMAANCTDAQRREELltiAE 231
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 240 KYGCDISRPAQNAKEAVQWTYFGYLAAVKSQNGAAMSFGRVSTFLDIYIQRDLDAGLlTEEQAQELIDHLVMKLRMVRFL 319
Cdd:PRK09983 232 ISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGE-DPAFLKELLESLWVKCNDIVLL 310
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 320 RTPEYDELFSGDPIWATESLGGMGLDGRTLVTKNTFRFLNTlYTMGPSPEPNMTILWSEQLPINF-KKYAAKVSIDTSSI 398
Cdd:PRK09983 311 RSTSSARYFAGFPTGYTALLGGLTENGRSAVNVLSFLCLDA-YQSVQLPQPNLGVRTNALIDTPFlMKTAETIRLGTGIP 389
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 399 QYENDDLMRPDF--------NSDDYAIACCVSPMVVGKQmqfFG----ARANLAKTLLYTI--NGGVDEklkiqvgpkha 464
Cdd:PRK09983 390 QIFNDEVVVPAFlnrgvsleDARDYSVVGCVELSIPGRT---YGlhdiAMFNLLKVMEICLheNEGNAA----------- 455
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 465 pimdevLDFDTVMNRMDQFMDWLATQYVTALNVIHYMHDKYSYEAALMAL------HDRDVY----RTMACGIAGLSVA- 533
Cdd:PRK09983 456 ------LTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFisdcleKGRDITdggaRYNFSGVQGIGIAn 529
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 534 -ADSLSAIKyakvSPIRDENGLAVD---------FNI-EGE---------YPQFGNNDSRVDDIACDLVERFMKKIQKLH 593
Cdd:PRK09983 530 lSDSLHALK----GMVFDQQRLSFDellsvlkanFATpEGEkvrarlinrFEKYGNDIDEVDNISAELLRHYCKEVEKYQ 605
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 594 TYRNAVPTQSILTITSNVVYGKKTGNTPDGRRAGAPFGPGA-NPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVP 672
Cdd:PRK09983 606 NPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGGlSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTP 685
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 673 NALGKDDDVRKanLAGLMDGYFHHEAdieggQHLNVNVMNREMLLEAMEDPEKYPQLTIRVSGYAVRFNSLTKEQQQDVI 752
Cdd:PRK09983 686 ATLEGEAGLRK--LADFLRAFTQLKL-----QHIQFNVVNADTLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDII 758
|
....*.
gi 490364911 753 TRTFTQ 758
Cdd:PRK09983 759 RRTAHQ 764
|
|
| PRK11127 |
PRK11127 |
autonomous glycyl radical cofactor GrcA; Provisional |
700-760 |
5.23e-38 |
|
autonomous glycyl radical cofactor GrcA; Provisional
Pssm-ID: 182983 [Multi-domain] Cd Length: 127 Bit Score: 137.67 E-value: 5.23e-38
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 490364911 700 IEGGQHLNVNVMNREMLLEAMEDPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQTM 760
Cdd:PRK11127 67 VEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVIARTFTESL 127
|
|
| GrcA |
COG3445 |
Autonomous glycyl radical cofactor GrcA [Coenzyme transport and metabolism]; |
696-760 |
3.34e-37 |
|
Autonomous glycyl radical cofactor GrcA [Coenzyme transport and metabolism];
Pssm-ID: 442669 [Multi-domain] Cd Length: 125 Bit Score: 135.25 E-value: 3.34e-37
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 490364911 696 HEADIEGGQHLNVNVMNREMLLEAMEDPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQTM 760
Cdd:COG3445 61 APPRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVITRTFTESL 125
|
|
| spare_glycyl |
TIGR04365 |
autonomous glycyl radical cofactor GrcA; This small protein, previously designated YfiD in E. ... |
696-760 |
1.31e-36 |
|
autonomous glycyl radical cofactor GrcA; This small protein, previously designated YfiD in E. coli, is closely homologous to pyruvate formate_lyase (PFL) in a region surrounding the stable glycyl radical that is prepared by the action of pyruvate formate-lyase activase, a radical SAM enzyme. When damage at the site of this radical breaks the main chain of PFL, this protein acts as a spare part that reintroduces the needed stable glycyl radical. Cutoffs for this model are set to exclude a set of closely related phage proteins that appear to have a corresponding function.
Pssm-ID: 213978 [Multi-domain] Cd Length: 124 Bit Score: 133.77 E-value: 1.31e-36
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 490364911 696 HEADIEGGQHLNVNVMNREMLLEAMEDPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQTM 760
Cdd:TIGR04365 60 APAKVEGGQHLNVNVLTRETLEDAVKNPEKYPQLTIRVSGYAVRFNSLTPEQQRDVITRTFTESL 124
|
|
| choline_CutC |
TIGR04394 |
choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and ... |
221-755 |
6.76e-34 |
|
choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and benzylsuccinate synthases, are glycine radical enzymes that appear to act as choline TMA-lyase, that is, to perform a C-N bond cleavage turning choline into trimethylamine (TMA) plus acetaldehyde. The gene symbol is cutC, for choline utilization. The activase, CutD, is a radical SAM enzyme. [Energy metabolism, Amino acids and amines]
Pssm-ID: 275187 [Multi-domain] Cd Length: 789 Bit Score: 139.16 E-value: 6.76e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 221 REEIAEQHAALGQIQEMAAKYGcdiSRPAQNAKEAVQ--WTYFGYLAAVKSQNGaaMSFGRVSTFLDIYIQRDLDAGLLT 298
Cdd:TIGR04394 228 KEQDPKRKAELEKIAEVNARVP---AHKPRTFWEAIQsvWTVESLLVVEENQTG--MSIGRVDQYMYPFYKADIEAGRMT 302
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 299 EEQAQELIDHLVMKLRMVRFLRTPEYDELFSGDPIWATESLGGMGLDGRTLVTKNTFRFLNTLYTMGPSpEPNMTILWSE 378
Cdd:TIGR04394 303 EYEAFELAGCMLIKMSEMMWLTSEGGSKFFAGYQPFVNMCVGGVTREGGDATNDLTYLLMDAVRHVKVY-QPSLACRIHN 381
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 379 QLPinfKKYAAK-VSIDTSSIQYEN---DD-----LMRPDF---NSDDYAIACCVSPMVVGKQMQFFG-ARANLAKTLLY 445
Cdd:TIGR04394 382 KSP---QKYLKKiVDVVRAGMGFPAchfDDahikmMLAKGVsieDARDYCLMGCVEPQKSGRLYQWTStAYTQWPICIEL 458
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 446 TINGGVDEKLKIQVGPKHAPI--MDEVLDFDTVMNRMDQFM-DWLATQYVTALNVIHYMHDKysyeaALMAL-------H 515
Cdd:TIGR04394 459 VLNHGVPLWYGKQVCPDTGDLsqFDTYEKFDAAVKEQIKYItKWSAVATVISQRVHRDLAPK-----PLMSLmyegcmeK 533
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 516 DRDVYRTMAC-----GI--AGLSVAADSLSAI--------KYAkVSPIRDenglAVDFNIEG---------EYPQFGNND 571
Cdd:TIGR04394 534 GKDVSAGGAMynfgpGVvwSGLATYADSMAAIkklvyddkKYT-LEQLNE----ALKANFEGyeqiradclDAPKYGNDD 608
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 572 SRVDDIACDLV---ERFMKKIQKLHTyrnaVPTQSILTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVAS 648
Cdd:TIGR04394 609 DYADLIAADLVnftEREHRKYKTLYS----HLSHGTLSISNNTPFGQLTGASANGRLAWTPLSDGISPTQGADFKGPTAI 684
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490364911 649 LTSVAKLPFAYAKDGISYTFSIVPNALgkDDDVRKANLAGLMdgyfhHEADIEGGQHLNVNVMNREMLLEAMEDPEKYPQ 728
Cdd:TIGR04394 685 IKSVSKMANDSMNIGMVHNFKLMSGLL--DTPEGENGLITLL-----RTASILGNGEMQFNYLDNETLLDAQQHPEKYRD 757
|
570 580
....*....|....*....|....*..
gi 490364911 729 LTIRVSGYAVRFNSLTKEQQQDVITRT 755
Cdd:TIGR04394 758 LVVRVAGYSAFFVELCKDVQDEIISRT 784
|
|
| |
