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Conserved domains on  [gi|490589203|ref|WP_004454223|]
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cell wall-binding protein Cwp25 [Clostridioides difficile]

Protein Classification

cell wall-binding repeat-containing protein( domain architecture ID 11454996)

cell wall-binding repeat-containing protein similar to Clostridium difficile S-layer protein SlpA, cell wall protein V (CwpV), and Cwp66; the cell wall binding repeat 2 is found in mutiple copies in proteins

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
LytB COG2247
Putative cell wall-binding domain (amidase enhancer), LytB superfamily [Cell wall/membrane ...
 35-303 8.52e-27

Putative cell wall-binding domain (amidase enhancer), LytB superfamily [Cell wall/membrane/envelope biogenesis];


:

Pssm-ID: 441848 [Multi-domain]  Cd Length: 472  Bit Score: 109.42  E-value: 8.52e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490589203  35 QGVDKYETAGLISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNR--VTKIYVIGGENSI 112
Cdd:COG2247  183 AAAGGAAGAVAIAKAGDSADTVVLARGDNFADALAAGPLAAALNAPILLTPSDSLPPATLAELKRlgPKKVYILGGTAAV 262

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490589203 113 SKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIekIKNSDKMFLVNGFkDEADAVSVASVAYRDGAPIILTKDIPSAEE 192
Cdd:COG2247  263 SEAVEDQLKALGITVERIAGADRYETAAAIAKEL--GPDADTVVLASGE-DFPDALSAAAAAAAAAAAAAAAAAAVAAAA 339

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490589203 193 DTDLDPWFGVSPVPVYAIGGESTLSDYIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKN 272
Cdd:COG2247  340 AAAAAAAAVAAAAGAALVAASAAAAAAAAAAGVAAAVAAGGAAGAAADAGAAAAAAAAAAAAAAAAAAAAAAAAAAAVAA 419

                        250       260       270
                 ....*....|....*....|....*....|.
gi 490589203 273 APVVLVSNKSDKSILSGASKVTAIGISDKSI 303
Cdd:COG2247  420 AGAAAAAAAAGGGAAAALLAAAGAGALAAAA 450
 
Name Accession Description Interval E-value
LytB COG2247
Putative cell wall-binding domain (amidase enhancer), LytB superfamily [Cell wall/membrane ...
 35-303 8.52e-27

Putative cell wall-binding domain (amidase enhancer), LytB superfamily [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441848 [Multi-domain]  Cd Length: 472  Bit Score: 109.42  E-value: 8.52e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490589203  35 QGVDKYETAGLISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNR--VTKIYVIGGENSI 112
Cdd:COG2247  183 AAAGGAAGAVAIAKAGDSADTVVLARGDNFADALAAGPLAAALNAPILLTPSDSLPPATLAELKRlgPKKVYILGGTAAV 262

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490589203 113 SKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIekIKNSDKMFLVNGFkDEADAVSVASVAYRDGAPIILTKDIPSAEE 192
Cdd:COG2247  263 SEAVEDQLKALGITVERIAGADRYETAAAIAKEL--GPDADTVVLASGE-DFPDALSAAAAAAAAAAAAAAAAAAVAAAA 339

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490589203 193 DTDLDPWFGVSPVPVYAIGGESTLSDYIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKN 272
Cdd:COG2247  340 AAAAAAAAVAAAAGAALVAASAAAAAAAAAAGVAAAVAAGGAAGAAADAGAAAAAAAAAAAAAAAAAAAAAAAAAAAVAA 419

                        250       260       270
                 ....*....|....*....|....*....|.
gi 490589203 273 APVVLVSNKSDKSILSGASKVTAIGISDKSI 303
Cdd:COG2247  420 AGAAAAAAAAGGGAAAALLAAAGAGALAAAA 450
CW_binding_2 pfam04122
ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide ...
 34-115 4.34e-15

ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide variety of bacterial cell surface proteins. It has been show the three tandem repeats of the CWB2 domain are essential for correct anchoring to the cell wall. It was shown that in SlpA and Cwp2 that these domains were essential for the binding of PSII an anionic teichoic acid-like component of the cell wall. The structure of the Cwp8 and Cwp6 proteins shows that this domain forms a trimeric arrangement with each domain adopting a structure with some similarity to the Toprim fold. A groove containing many conserved residues was predicted to be the site of the PSII molecule.


Pssm-ID: 461185 [Multi-domain]  Cd Length: 80  Bit Score: 69.09  E-value: 4.34e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490589203   34 IQGVDKYETAGLISDKQDFTTAVLINADStMVDGIAASGLAGVNNAAILLTNKDDIPEatLQRLNRVTKIYVIGGENSIS 113
Cdd:pfam04122   2 LAGSDRYETAAKIAKELGWDTVVVASGEN-FADALSAAPLAAKKNAPILLTDKNSLDS--LKKALKAELVYILGGTGVIS 78


                  ..
gi 490589203  114 KD 115
Cdd:pfam04122  79 DS 80
 
Name Accession Description Interval E-value
LytB COG2247
Putative cell wall-binding domain (amidase enhancer), LytB superfamily [Cell wall/membrane ...
 35-303 8.52e-27

Putative cell wall-binding domain (amidase enhancer), LytB superfamily [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441848 [Multi-domain]  Cd Length: 472  Bit Score: 109.42  E-value: 8.52e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490589203  35 QGVDKYETAGLISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNR--VTKIYVIGGENSI 112
Cdd:COG2247  183 AAAGGAAGAVAIAKAGDSADTVVLARGDNFADALAAGPLAAALNAPILLTPSDSLPPATLAELKRlgPKKVYILGGTAAV 262

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490589203 113 SKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIekIKNSDKMFLVNGFkDEADAVSVASVAYRDGAPIILTKDIPSAEE 192
Cdd:COG2247  263 SEAVEDQLKALGITVERIAGADRYETAAAIAKEL--GPDADTVVLASGE-DFPDALSAAAAAAAAAAAAAAAAAAVAAAA 339

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490589203 193 DTDLDPWFGVSPVPVYAIGGESTLSDYIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKN 272
Cdd:COG2247  340 AAAAAAAAVAAAAGAALVAASAAAAAAAAAAGVAAAVAAGGAAGAAADAGAAAAAAAAAAAAAAAAAAAAAAAAAAAVAA 419

                        250       260       270
                 ....*....|....*....|....*....|.
gi 490589203 273 APVVLVSNKSDKSILSGASKVTAIGISDKSI 303
Cdd:COG2247  420 AGAAAAAAAAGGGAAAALLAAAGAGALAAAA 450
CW_binding_2 pfam04122
ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide ...
 34-115 4.34e-15

ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide variety of bacterial cell surface proteins. It has been show the three tandem repeats of the CWB2 domain are essential for correct anchoring to the cell wall. It was shown that in SlpA and Cwp2 that these domains were essential for the binding of PSII an anionic teichoic acid-like component of the cell wall. The structure of the Cwp8 and Cwp6 proteins shows that this domain forms a trimeric arrangement with each domain adopting a structure with some similarity to the Toprim fold. A groove containing many conserved residues was predicted to be the site of the PSII molecule.


Pssm-ID: 461185 [Multi-domain]  Cd Length: 80  Bit Score: 69.09  E-value: 4.34e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490589203   34 IQGVDKYETAGLISDKQDFTTAVLINADStMVDGIAASGLAGVNNAAILLTNKDDIPEatLQRLNRVTKIYVIGGENSIS 113
Cdd:pfam04122   2 LAGSDRYETAAKIAKELGWDTVVVASGEN-FADALSAAPLAAKKNAPILLTDKNSLDS--LKKALKAELVYILGGTGVIS 78


                  ..
gi 490589203  114 KD 115
Cdd:pfam04122  79 DS 80
CW_binding_2 pfam04122
ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide ...
 228-301 1.08e-13

ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide variety of bacterial cell surface proteins. It has been show the three tandem repeats of the CWB2 domain are essential for correct anchoring to the cell wall. It was shown that in SlpA and Cwp2 that these domains were essential for the binding of PSII an anionic teichoic acid-like component of the cell wall. The structure of the Cwp8 and Cwp6 proteins shows that this domain forms a trimeric arrangement with each domain adopting a structure with some similarity to the Toprim fold. A groove containing many conserved residues was predicted to be the site of the PSII molecule.


Pssm-ID: 461185 [Multi-domain]  Cd Length: 80  Bit Score: 65.24  E-value: 1.08e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490589203  228 RIGGVDRYQTNKNVIEKFYNGAKefYITSGDDLVYALVASPLA--KNAPVVLVSNKSDKSILSG--ASKVTAIG----IS 299
Cdd:pfam04122   1 RLAGSDRYETAAKIAKELGWDTV--VVASGENFADALSAAPLAakKNAPILLTDKNSLDSLKKAlkAELVYILGgtgvIS 78


                  ..
gi 490589203  300 DK 301
Cdd:pfam04122  79 DS 80
CW_binding_2 pfam04122
ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide ...
 129-218 2.49e-13

ell wall binding domain 2 (CWB2); This domain is found in 1 to 3 tandem copies in a wide variety of bacterial cell surface proteins. It has been show the three tandem repeats of the CWB2 domain are essential for correct anchoring to the cell wall. It was shown that in SlpA and Cwp2 that these domains were essential for the binding of PSII an anionic teichoic acid-like component of the cell wall. The structure of the Cwp8 and Cwp6 proteins shows that this domain forms a trimeric arrangement with each domain adopting a structure with some similarity to the Toprim fold. A groove containing many conserved residues was predicted to be the site of the PSII molecule.


Pssm-ID: 461185 [Multi-domain]  Cd Length: 80  Bit Score: 64.47  E-value: 2.49e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490589203  129 RIDGVDRVDTSYKIAgeieKIKNSDKMFLVNGfKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFgvspvpVY 208
Cdd:pfam04122   1 RLAGSDRYETAAKIA----KELGWDTVVVASG-ENFADALSAAPLAAKKNAPILLTDKNSLDSLKKALKAEL------VY 69

                          90
                  ....*....|
gi 490589203  209 AIGGESTLSD 218
Cdd:pfam04122  70 ILGGTGVISD 79
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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