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Conserved domains on  [gi|490995469|ref|WP_004857197|]
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MULTISPECIES: GNAT family N-acetyltransferase [Raoultella]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10456837)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  15581578|10940244|9175471|27367672|26818562
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 26-125 3.84e-08

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


:

Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 49.05  E-value: 3.84e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490995469   26 FPWHEQREPAAKQQALRDPRYALEAWFDGEL--FIGLSGCWQFSGYSYIEHLAIDDTLRSRGYGKRLLAGILERAP---L 100
Cdd:pfam00583  14 EPWPDEPLDLLEDWDEDASEGFFVAEEDGELvgFASLSIIDDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARergC 93

                          90       100
                  ....*....|....*....|....*
gi 490995469  101 TILEIDPLTSDiaHKRLRFYETMGF 125
Cdd:pfam00583  94 ERIFLEVAADN--LAAIALYEKLGF 116
 
Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 26-125 3.84e-08

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 49.05  E-value: 3.84e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490995469   26 FPWHEQREPAAKQQALRDPRYALEAWFDGEL--FIGLSGCWQFSGYSYIEHLAIDDTLRSRGYGKRLLAGILERAP---L 100
Cdd:pfam00583  14 EPWPDEPLDLLEDWDEDASEGFFVAEEDGELvgFASLSIIDDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARergC 93

                          90       100
                  ....*....|....*....|....*
gi 490995469  101 TILEIDPLTSDiaHKRLRFYETMGF 125
Cdd:pfam00583  94 ERIFLEVAADN--LAAIALYEKLGF 116
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
17-129 2.29e-07

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 47.77  E-value: 2.29e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490995469  17 AVDALYVKAFPWHEQREPAAKQQALRDPRYALEAWFDGEL--FIGLSGCWQFSGYS--YIEHLAIDDTLRSRGYGKRLLA 92
Cdd:COG3153   11 AIAALLRAAFGPGREAELVDRLREDPAAGLSLVAEDDGEIvgHVALSPVDIDGEGPalLLGPLAVDPEYRGQGIGRALMR 90

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 490995469  93 GILERA-----PLTILEIDPltsdiahKRLRFYETMGFRASS 129
Cdd:COG3153   91 AALEAArergaRAVVLLGDP-------SLLPFYERFGFRPAG 125
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 50-98 1.09e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.80  E-value: 1.09e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 490995469  50 AWFDGEL--FIGLSGCWQFSGYSYIEHLAIDDTLRSRGYGKRLLAGILERA 98
Cdd:cd04301    4 AEDDGEIvgFASLSPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEA 54
PRK10514 PRK10514
putative acetyltransferase; Provisional
 70-131 8.63e-03

putative acetyltransferase; Provisional


Pssm-ID: 182510 [Multi-domain]  Cd Length: 145  Bit Score: 34.98  E-value: 8.63e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 490995469  70 SYIEHLAIDDTLRSRGYGKRLLAGILERAPLtileidpLTSDIA---HKRLRFYETMGFRASSWS 131
Cdd:PRK10514  70 GHMEALFVDPDVRGCGVGRMLVEHALSLHPE-------LTTDVNeqnEQAVGFYKKMGFKVTGRS 127
 
Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 26-125 3.84e-08

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 49.05  E-value: 3.84e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490995469   26 FPWHEQREPAAKQQALRDPRYALEAWFDGEL--FIGLSGCWQFSGYSYIEHLAIDDTLRSRGYGKRLLAGILERAP---L 100
Cdd:pfam00583  14 EPWPDEPLDLLEDWDEDASEGFFVAEEDGELvgFASLSIIDDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARergC 93

                          90       100
                  ....*....|....*....|....*
gi 490995469  101 TILEIDPLTSDiaHKRLRFYETMGF 125
Cdd:pfam00583  94 ERIFLEVAADN--LAAIALYEKLGF 116
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
17-129 2.29e-07

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 47.77  E-value: 2.29e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490995469  17 AVDALYVKAFPWHEQREPAAKQQALRDPRYALEAWFDGEL--FIGLSGCWQFSGYS--YIEHLAIDDTLRSRGYGKRLLA 92
Cdd:COG3153   11 AIAALLRAAFGPGREAELVDRLREDPAAGLSLVAEDDGEIvgHVALSPVDIDGEGPalLLGPLAVDPEYRGQGIGRALMR 90

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 490995469  93 GILERA-----PLTILEIDPltsdiahKRLRFYETMGFRASS 129
Cdd:COG3153   91 AALEAArergaRAVVLLGDP-------SLLPFYERFGFRPAG 125
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 28-129 8.39e-07

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 45.72  E-value: 8.39e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 490995469   28 WHEQREPAAKQQALRDPRYALEAWFDGELFIGLSGcwqFSGYSYIEHLAIDDTLRSRGYGKRLLAGILERAPLTILEIDP 107
Cdd:pfam13673  13 FYEFISPEALRERIDQGEYFFFVAFEGGQIVGVIA---LRDRGHISLLFVDPDYQGQGIGKALLEAVEDYAEKDGIKLSE 89

                          90       100
                  ....*....|....*....|..
gi 490995469  108 LTSDIAHKRLRFYETMGFRASS 129
Cdd:pfam13673  90 LTVNASPYAVPFYEKLGFRATG 111
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 50-126 4.66e-05

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 40.13  E-value: 4.66e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 490995469   50 AWFDGELfIGLSGCWQFSGYSYIEH--LAIDDTLRSRGYGKRLLAGILERAPLTILEIDPLTSDiaHKRLRFYETMGFR 126
Cdd:pfam13508   8 AEDDGKI-VGFAALLPLDDEGALAElrLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETT--NRAAAFYEKLGFE 83
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 50-98 1.09e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.80  E-value: 1.09e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 490995469  50 AWFDGEL--FIGLSGCWQFSGYSYIEHLAIDDTLRSRGYGKRLLAGILERA 98
Cdd:cd04301    4 AEDDGEIvgFASLSPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEA 54
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 50-126 2.89e-04

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 38.82  E-value: 2.89e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 490995469  50 AWFDGEL--FIGLSGCWQfsGYSYIEHLAIDDTLRSRGYGKRLLAGILERAplTILEIDPLTSDIAHKRLRFYETMGFR 126
Cdd:COG1246   33 AEEDGEIvgCAALHPLDE--DLAELRSLAVHPDYRGRGIGRRLLEALLAEA--RELGLKRLFLLTTSAAIHFYEKLGFE 107
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 68-126 3.48e-04

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 38.10  E-value: 3.48e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 490995469  68 GYSYIEHLAIDDTLRSRGYGKRLLAGILERAP---LTILEIDPLTSDIAHkrLRFYETMGFR 126
Cdd:COG0456   12 DEAEIEDLAVDPEYRGRGIGRALLEAALERARergARRLRLEVREDNEAA--IALYEKLGFE 71
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 58-127 6.30e-03

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 35.03  E-value: 6.30e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 490995469  58 IGLSGCWQFSG-YSYIEHLAIDDTLRSRGYGKRLLAGILERAP---LTILEIDplTSDIAHKRLRFYETMGFRA 127
Cdd:COG0454   46 IGFAGLRRLDDkVLELKRLYVLPEYRGKGIGKALLEALLEWARergCTALELD--TLDGNPAAIRFYERLGFKE 117
PRK10514 PRK10514
putative acetyltransferase; Provisional
 70-131 8.63e-03

putative acetyltransferase; Provisional


Pssm-ID: 182510 [Multi-domain]  Cd Length: 145  Bit Score: 34.98  E-value: 8.63e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 490995469  70 SYIEHLAIDDTLRSRGYGKRLLAGILERAPLtileidpLTSDIA---HKRLRFYETMGFRASSWS 131
Cdd:PRK10514  70 GHMEALFVDPDVRGCGVGRMLVEHALSLHPE-------LTTDVNeqnEQAVGFYKKMGFKVTGRS 127
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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