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Conserved domains on  [gi|491118342|ref|WP_004976793|]
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MULTISPECIES: type II secretion system minor pseudopilin GspI [Acinetobacter]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PilV COG4967
Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];
2-61 2.05e-09

Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];


:

Pssm-ID: 443993 [Multi-domain]  Cd Length: 86  Bit Score: 50.75  E-value: 2.05e-09
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 491118342   2 KAKGFTLLEVMVALAIFATAAMAMTKVAMQYTQSTANAILRTKAQFVALNEVALMEMNRQ 61
Cdd:COG4967    9 RQRGFTLIEVLVALVILSIGLLGLAGLQAASLRSSQDARQRTQAALLAQDLLERLRANPA 68
T2SSI super family cl38073
Type II secretion system (T2SS), protein I; The Type II secretion system, also called ...
 42-117 6.20e-05

Type II secretion system (T2SS), protein I; The Type II secretion system, also called Secretion-dependent pathway (SDP), is responsible for the transport of proteins across the outer membrane first exported to the periplasm by the Sec or Tat translocon in Gram-negative (diderm) bacteria. As members of the T2SJ family, members of the T2SI family are pseudopilins containing prepilin signal sequences.


The actual alignment was detected with superfamily member pfam02501:

Pssm-ID: 426802  Cd Length: 80  Bit Score: 38.78  E-value: 6.20e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 491118342   42 RTKAQFVALNEVALMEMNRQWLS-GTESKQVTQQGETWQIDKSTQPTISPNVQRVDLQISLYNQDTGQVeAGVTSLV 117
Cdd:pfam02501   4 RTLAQWVAENQLAELRLEPQWPAiGESSGECEQAGRRWYWRVEVVPTPDPGFRRVDVSVRADKDEARPL-ASLRTYV 79
 
Name Accession Description Interval E-value
PilV COG4967
Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];
2-61 2.05e-09

Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];


Pssm-ID: 443993 [Multi-domain]  Cd Length: 86  Bit Score: 50.75  E-value: 2.05e-09
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 491118342   2 KAKGFTLLEVMVALAIFATAAMAMTKVAMQYTQSTANAILRTKAQFVALNEVALMEMNRQ 61
Cdd:COG4967    9 RQRGFTLIEVLVALVILSIGLLGLAGLQAASLRSSQDARQRTQAALLAQDLLERLRANPA 68
gspI TIGR01707
type II secretion system protein I; This model represents GspI, one of two proteins highly ...
 5-101 1.09e-07

type II secretion system protein I; This model represents GspI, one of two proteins highly conserved at their N-termini and described by pfam02501 but easily separable phylogenetically. The other is GspJ. Both GspI and GspJ are proteins of the type II secretion pathway, or main terminal branch of the general secretion pathway. This pathway carries proteins across the outer membrane. Note that proteins of type II secretion are cryptic in E. coli K-12 - present but not yet demonstrated to act on any target.


Pssm-ID: 273767 [Multi-domain]  Cd Length: 101  Bit Score: 46.36  E-value: 1.09e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491118342    5 GFTLLEVMVALAIFATAAMAMTKVAMQYTQSTANAILRTKAQFVALNEVALMEMNRQWLSGTESKQVTQ-QGETWQIDKS 83
Cdd:TIGR01707   1 GFTLLEVLVALAIFAAAALALISSVGGQTNAIGRLRDKTLALWIADNRLAELSLGVGPPALGRNSGKELiAGREWLWRSQ 80

                          90
                  ....*....|....*...
gi 491118342   84 TQPTISPNVQRVDLQISL 101
Cdd:TIGR01707  81 VHSTDDPTLLRVDITVAT 98
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 1-26 1.88e-05

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 38.89  E-value: 1.88e-05
                          10        20
                  ....*....|....*....|....*.
gi 491118342    1 MKAKGFTLLEVMVALAIFATAAMAMT 26
Cdd:pfam07963   2 RKQRGFTLIELLVALAILAILLAAAL 27
T2SSI pfam02501
Type II secretion system (T2SS), protein I; The Type II secretion system, also called ...
 42-117 6.20e-05

Type II secretion system (T2SS), protein I; The Type II secretion system, also called Secretion-dependent pathway (SDP), is responsible for the transport of proteins across the outer membrane first exported to the periplasm by the Sec or Tat translocon in Gram-negative (diderm) bacteria. As members of the T2SJ family, members of the T2SI family are pseudopilins containing prepilin signal sequences.


Pssm-ID: 426802  Cd Length: 80  Bit Score: 38.78  E-value: 6.20e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 491118342   42 RTKAQFVALNEVALMEMNRQWLS-GTESKQVTQQGETWQIDKSTQPTISPNVQRVDLQISLYNQDTGQVeAGVTSLV 117
Cdd:pfam02501   4 RTLAQWVAENQLAELRLEPQWPAiGESSGECEQAGRRWYWRVEVVPTPDPGFRRVDVSVRADKDEARPL-ASLRTYV 79
T4P_ComGE NF041013
competence type IV pilus minor pilin ComGE;
4-73 4.44e-04

competence type IV pilus minor pilin ComGE;


Pssm-ID: 468942 [Multi-domain]  Cd Length: 83  Bit Score: 36.41  E-value: 4.44e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491118342   4 KGFTLLEVMVALAIFATAAMAMTKVAMQYTQSTANAILRTKAQFvalneVALMEMNRQWLSGTESKQVTQ 73
Cdd:NF041013   4 KGFILLESLVALALLALIVSLLLPSLTQIQKEREEIKQKEEALQ-----VLYEALQTYQLHLELNGVEIT 68
PRK10557 PRK10557
prepilin peptidase-dependent protein;
1-28 1.11e-03

prepilin peptidase-dependent protein;


Pssm-ID: 236714  Cd Length: 192  Bit Score: 36.88  E-value: 1.11e-03
                         10        20
                 ....*....|....*....|....*...
gi 491118342   1 MKAKGFTLLEVMVALAIFATAAMAMTKV 28
Cdd:PRK10557   4 VKQRGFSLLEVLLAMAIGSVLLLGAARF 31
pilin_ComGF NF041002
competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type ...
 4-38 4.74e-03

competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type IV pilus involved in the competence system of natural transformation by various Gram-positive bacteria. Oddly, while nearly all members have an N-terminal region with a consensus site for cleavage and methylation, ComGF from Bacillus subtilis appears N-terminally truncated, and may be functional despite the truncation.


Pssm-ID: 468931 [Multi-domain]  Cd Length: 138  Bit Score: 34.51  E-value: 4.74e-03
                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 491118342   4 KGFTLLEVMVALAIFATAAM---AMTKVAMQYTQSTAN 38
Cdd:NF041002   2 KGFTLLECLVALLVLSLILLlipLLIKLLLKVEQYLNN 39
 
Name Accession Description Interval E-value
PilV COG4967
Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];
2-61 2.05e-09

Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];


Pssm-ID: 443993 [Multi-domain]  Cd Length: 86  Bit Score: 50.75  E-value: 2.05e-09
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 491118342   2 KAKGFTLLEVMVALAIFATAAMAMTKVAMQYTQSTANAILRTKAQFVALNEVALMEMNRQ 61
Cdd:COG4967    9 RQRGFTLIEVLVALVILSIGLLGLAGLQAASLRSSQDARQRTQAALLAQDLLERLRANPA 68
gspI TIGR01707
type II secretion system protein I; This model represents GspI, one of two proteins highly ...
 5-101 1.09e-07

type II secretion system protein I; This model represents GspI, one of two proteins highly conserved at their N-termini and described by pfam02501 but easily separable phylogenetically. The other is GspJ. Both GspI and GspJ are proteins of the type II secretion pathway, or main terminal branch of the general secretion pathway. This pathway carries proteins across the outer membrane. Note that proteins of type II secretion are cryptic in E. coli K-12 - present but not yet demonstrated to act on any target.


Pssm-ID: 273767 [Multi-domain]  Cd Length: 101  Bit Score: 46.36  E-value: 1.09e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491118342    5 GFTLLEVMVALAIFATAAMAMTKVAMQYTQSTANAILRTKAQFVALNEVALMEMNRQWLSGTESKQVTQ-QGETWQIDKS 83
Cdd:TIGR01707   1 GFTLLEVLVALAIFAAAALALISSVGGQTNAIGRLRDKTLALWIADNRLAELSLGVGPPALGRNSGKELiAGREWLWRSQ 80

                          90
                  ....*....|....*...
gi 491118342   84 TQPTISPNVQRVDLQISL 101
Cdd:TIGR01707  81 VHSTDDPTLLRVDITVAT 98
PulG COG2165
Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, ...
 1-50 1.55e-06

Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 441768 [Multi-domain]  Cd Length: 99  Bit Score: 43.36  E-value: 1.55e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 491118342   1 MKAKGFTLLEVMVALAIFATAAMAMTKVAMQYTQSTANAILRTKAQFVAL 50
Cdd:COG2165    8 RRQRGFTLIELLVVIAIIGILAALALPALQGARERARRAELRSNLRQIQQ 57
type_IV_pilV TIGR02523
type IV pilus modification protein PilV; Pilus systems categorized as type IV pilins differ ...
 3-64 3.69e-06

type IV pilus modification protein PilV; Pilus systems categorized as type IV pilins differ greatly from one another, with some showing greater similarty to type II or type III secretion systems than to each other. Members of this protein family represent the PilV protein of type IV pilus systems as found in Pseudomonas aeruginosa PAO1, Pseudomonas syringae DC3000, Neisseria meningitidis MC58, Xylella fastidiosa 9a5c, etc. [Cell envelope, Surface structures, Protein fate, Protein modification and repair]


Pssm-ID: 131575  Cd Length: 139  Bit Score: 43.22  E-value: 3.69e-06
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 491118342    3 AKGFTLLEVMVALAIFATAAMAMTKVAMQYTQSTANAILRTKAQFVALNEVALMEMNRQWLS 64
Cdd:TIGR02523   1 QAGFSMIEVLVALLVLAIGVLGMAALQLKAVRYTRSASTRTIASMLAYNLLDRMRANPTALG 62
PulJ COG4795
Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and ...
 1-56 1.83e-05

Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443823 [Multi-domain]  Cd Length: 118  Bit Score: 40.77  E-value: 1.83e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 491118342   1 MKAKGFTLLEVMVALAIFATAAMAmtkvAMQYTQSTANAILRTKAQFVALNEVALM 56
Cdd:COG4795    6 RRQRGFTLLELLVALAIFALLLLA----AYRGLDSVLRSRERLEQQAERLQELQRA 57
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 1-26 1.88e-05

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 38.89  E-value: 1.88e-05
                          10        20
                  ....*....|....*....|....*.
gi 491118342    1 MKAKGFTLLEVMVALAIFATAAMAMT 26
Cdd:pfam07963   2 RKQRGFTLIELLVALAILAILLAAAL 27
T2SSI pfam02501
Type II secretion system (T2SS), protein I; The Type II secretion system, also called ...
 42-117 6.20e-05

Type II secretion system (T2SS), protein I; The Type II secretion system, also called Secretion-dependent pathway (SDP), is responsible for the transport of proteins across the outer membrane first exported to the periplasm by the Sec or Tat translocon in Gram-negative (diderm) bacteria. As members of the T2SJ family, members of the T2SI family are pseudopilins containing prepilin signal sequences.


Pssm-ID: 426802  Cd Length: 80  Bit Score: 38.78  E-value: 6.20e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 491118342   42 RTKAQFVALNEVALMEMNRQWLS-GTESKQVTQQGETWQIDKSTQPTISPNVQRVDLQISLYNQDTGQVeAGVTSLV 117
Cdd:pfam02501   4 RTLAQWVAENQLAELRLEPQWPAiGESSGECEQAGRRWYWRVEVVPTPDPGFRRVDVSVRADKDEARPL-ASLRTYV 79
gspJ TIGR01711
type II secretion system protein J; This model represents GspJ, one of two proteins highly ...
 5-81 1.16e-04

type II secretion system protein J; This model represents GspJ, one of two proteins highly conserved at their N-termini and described by pfam02501 but easily separable phylogenetically. The other is GspI. Both GspI and GspJ are proteins of the type II secretion pathway, or main terminal branch of the general secretion pathway. This pathway carries proteins across the outer membrane. Note that proteins of type II secretion are cryptic in E. coli K-12 - present but not yet demonstrated to act on any target.


Pssm-ID: 130772 [Multi-domain]  Cd Length: 192  Bit Score: 39.90  E-value: 1.16e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 491118342    5 GFTLLEVMVALAIFATAAMAMTKVAMQYTQStaNAILRTKAQFVALNEVALMEMNRQWLSGTEsKQVTQQGETWQID 81
Cdd:TIGR01711   2 GFTLLELLVAIAIFASLSLGAYQVLDSVMQS--DEATRVQEARLRELQRAMGAMERDLTQMVE-RPVRDDGEASEQD 75
T4P_ComGE NF041013
competence type IV pilus minor pilin ComGE;
4-73 4.44e-04

competence type IV pilus minor pilin ComGE;


Pssm-ID: 468942 [Multi-domain]  Cd Length: 83  Bit Score: 36.41  E-value: 4.44e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491118342   4 KGFTLLEVMVALAIFATAAMAMTKVAMQYTQSTANAILRTKAQFvalneVALMEMNRQWLSGTESKQVTQ 73
Cdd:NF041013   4 KGFILLESLVALALLALIVSLLLPSLTQIQKEREEIKQKEEALQ-----VLYEALQTYQLHLELNGVEIT 68
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 3-26 5.54e-04

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 34.97  E-value: 5.54e-04
                          10        20
                  ....*....|....*....|....
gi 491118342    3 AKGFTLLEVMVALAIFATAAMAMT 26
Cdd:TIGR02532   1 QRGFTLIELLVVLAILGILALIAL 24
PRK10557 PRK10557
prepilin peptidase-dependent protein;
1-28 1.11e-03

prepilin peptidase-dependent protein;


Pssm-ID: 236714  Cd Length: 192  Bit Score: 36.88  E-value: 1.11e-03
                         10        20
                 ....*....|....*....|....*...
gi 491118342   1 MKAKGFTLLEVMVALAIFATAAMAMTKV 28
Cdd:PRK10557   4 VKQRGFSLLEVLLAMAIGSVLLLGAARF 31
pilin_ComGF NF041002
competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type ...
 4-38 4.74e-03

competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type IV pilus involved in the competence system of natural transformation by various Gram-positive bacteria. Oddly, while nearly all members have an N-terminal region with a consensus site for cleavage and methylation, ComGF from Bacillus subtilis appears N-terminally truncated, and may be functional despite the truncation.


Pssm-ID: 468931 [Multi-domain]  Cd Length: 138  Bit Score: 34.51  E-value: 4.74e-03
                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 491118342   4 KGFTLLEVMVALAIFATAAM---AMTKVAMQYTQSTAN 38
Cdd:NF041002   2 KGFTLLECLVALLVLSLILLlipLLIKLLLKVEQYLNN 39
PilE COG4968
Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];
1-39 6.67e-03

Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];


Pssm-ID: 443994 [Multi-domain]  Cd Length: 124  Bit Score: 34.28  E-value: 6.67e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|
gi 491118342   1 MKAKGFTLLEVMVALAIFAT-AAMAMTkvamQYTQSTANA 39
Cdd:COG4968    7 RRQRGFTLIELMIVVAIIGIlAAIAIP----SYQDYVERA 42
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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