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Conserved domains on  [gi|491457357|ref|WP_005315136|]
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hydrogenase maturation nickel metallochaperone HypA [Aeromonas salmonicida]

Protein Classification

hydrogenase maturation nickel metallochaperone HypA( domain architecture ID 10001253)

hydrogenase maturation nickel metallochaperone HypA is required for nickel insertion into the metal center of the hydrogenase

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
HybF COG0375
Hydrogenase maturation factor HypA/HybF, metallochaperone involved in Ni insertion ...
 1-113 4.53e-48

Hydrogenase maturation factor HypA/HybF, metallochaperone involved in Ni insertion [Posttranslational modification, protein turnover, chaperones];


:

Pssm-ID: 440144  Cd Length: 113  Bit Score: 149.16  E-value: 4.53e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491457357   1 MHEMSLAVAAIDLAAEQATQRGFNKVTALWLEVGSFSCVDPDTIAFCFEAAAKGTVVEGAQLHFQHQAAEAWCYDCDKTV 80
Cdd:COG0375    1 MHELSIAQSILEIVEEEARENGAKRVTKVRLEIGELSGVEPEALEFAFEVLARGTIAEGAELEIEEVPARARCRDCGKEF 80

                         90       100       110
                 ....*....|....*....|....*....|...
gi 491457357  81 TLTERGQACPECGGYKLRVAQGDSLRITDIEVS 113
Cdd:COG0375   81 ELEELFLRCPKCGSPDLEILSGRELRIKSIEVE 113
 
Name Accession Description Interval E-value
HybF COG0375
Hydrogenase maturation factor HypA/HybF, metallochaperone involved in Ni insertion ...
 1-113 4.53e-48

Hydrogenase maturation factor HypA/HybF, metallochaperone involved in Ni insertion [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440144  Cd Length: 113  Bit Score: 149.16  E-value: 4.53e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491457357   1 MHEMSLAVAAIDLAAEQATQRGFNKVTALWLEVGSFSCVDPDTIAFCFEAAAKGTVVEGAQLHFQHQAAEAWCYDCDKTV 80
Cdd:COG0375    1 MHELSIAQSILEIVEEEARENGAKRVTKVRLEIGELSGVEPEALEFAFEVLARGTIAEGAELEIEEVPARARCRDCGKEF 80

                         90       100       110
                 ....*....|....*....|....*....|...
gi 491457357  81 TLTERGQACPECGGYKLRVAQGDSLRITDIEVS 113
Cdd:COG0375   81 ELEELFLRCPKCGSPDLEILSGRELRIKSIEVE 113
HypA pfam01155
Hydrogenase/urease nickel incorporation, metallochaperone, hypA; HypA is a metallochaperone ...
 2-112 7.50e-45

Hydrogenase/urease nickel incorporation, metallochaperone, hypA; HypA is a metallochaperone that binds nickel to bring it safely to its target. The targets for Hypa are the nickel-containing enzymes [Ni,Fe]-hydrogenase and urease. The nickel coordinates with four nitrogens within the protein. The four conserved cysteines towards the C-terminus bind one zinc moiety probably to stabilize the protein fold.


Pssm-ID: 460085  Cd Length: 111  Bit Score: 141.12  E-value: 7.50e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491457357    2 HEMSLAVAAIDLAAEQATQRGFNKVTALWLEVGSFSCVDPDTIAFCFEAAAKGTVVEGAQLHFQHQAAEAWCYDCDKTVT 81
Cdd:pfam01155   1 HELSIAQSIVEIVEEEAKEHGATRVTKVTLEVGELSGVEPEALRFAFEVAAKGTLAEGAELEIEEVPARARCRDCGKEFE 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 491457357   82 LTERGQACPECGGYKLRVAQGDSLRITDIEV 112
Cdd:pfam01155  81 LEERDLVCPRCGSPDVEILSGRELRIKSIEV 111
PRK12380 PRK12380
hydrogenase/urease nickel incorporation protein;
1-112 6.47e-35

hydrogenase/urease nickel incorporation protein;


Pssm-ID: 183485  Cd Length: 113  Bit Score: 115.83  E-value: 6.47e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491457357   1 MHEMSLAVAAIDLAAEQATQRGFNKVTALWLEVGSFSCVDPDTIAFCFEAAAKGTVVEGAQLHFQHQAAEAWCYDCDKTV 80
Cdd:PRK12380   1 MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVV 80

                         90       100       110
                 ....*....|....*....|....*....|..
gi 491457357  81 TLTERGQACPECGGYKLRVAQGDSLRITDIEV 112
Cdd:PRK12380  81 EIHQHDAQCPHCHGERLRVDTGDSLIVKSIEV 112
hypA TIGR00100
hydrogenase nickel insertion protein HypA; CXXC-~12X-CXXC and genetically seems a regulatory ...
 1-113 3.61e-34

hydrogenase nickel insertion protein HypA; CXXC-~12X-CXXC and genetically seems a regulatory protein. In Hpylori, hypA mutant abolished hydrogenase activity and decrease in urease activity. Nickel supplementation in media restored urease activity and partial hydrogenase activity. HypA probably involved in inserting Ni in enzymes. [Protein fate, Protein modification and repair]


Pssm-ID: 272906  Cd Length: 115  Bit Score: 114.01  E-value: 3.61e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491457357    1 MHEMSLAVAAIDLAAEQATQRGFNKVTALWLEVGSFSCVDPDTIAFCFEAAAKGTVVEGAQLHFQHQAAEAWCYDCDKTV 80
Cdd:TIGR00100   1 MHELSLAEAMLEIVEEQAEKHQAKKVTRVTLEIGELSCVNPSQLQFAFEVVREGTVAEGAKLNIEDEPVECECEDCSEEV 80

                          90       100       110
                  ....*....|....*....|....*....|...
gi 491457357   81 TLTERGQACPECGGYKLRVAQGDSLRITDIEVS 113
Cdd:TIGR00100  81 SPEIDLYRCPKCHGIMLQVRAGKELNLKSIEVE 113
rubre_like_arch NF033497
rubrerythrin-like domain; This rubrerythrin-like domain is found primarily in the archaea, ...
 73-94 4.41e-03

rubrerythrin-like domain; This rubrerythrin-like domain is found primarily in the archaea, occasionally as part of a larger redox-active protein. It features two CxxC motifs with a spacer of 12 to 13 amino acids.


Pssm-ID: 411139  Cd Length: 34  Bit Score: 32.62  E-value: 4.41e-03
                         10        20
                 ....*....|....*....|..
gi 491457357  73 CYDCDKTVTLTERGQACPECGG 94
Cdd:NF033497   3 CFDCGTRVTAATHPGTCPDCGG 24
 
Name Accession Description Interval E-value
HybF COG0375
Hydrogenase maturation factor HypA/HybF, metallochaperone involved in Ni insertion ...
 1-113 4.53e-48

Hydrogenase maturation factor HypA/HybF, metallochaperone involved in Ni insertion [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440144  Cd Length: 113  Bit Score: 149.16  E-value: 4.53e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491457357   1 MHEMSLAVAAIDLAAEQATQRGFNKVTALWLEVGSFSCVDPDTIAFCFEAAAKGTVVEGAQLHFQHQAAEAWCYDCDKTV 80
Cdd:COG0375    1 MHELSIAQSILEIVEEEARENGAKRVTKVRLEIGELSGVEPEALEFAFEVLARGTIAEGAELEIEEVPARARCRDCGKEF 80

                         90       100       110
                 ....*....|....*....|....*....|...
gi 491457357  81 TLTERGQACPECGGYKLRVAQGDSLRITDIEVS 113
Cdd:COG0375   81 ELEELFLRCPKCGSPDLEILSGRELRIKSIEVE 113
HypA pfam01155
Hydrogenase/urease nickel incorporation, metallochaperone, hypA; HypA is a metallochaperone ...
 2-112 7.50e-45

Hydrogenase/urease nickel incorporation, metallochaperone, hypA; HypA is a metallochaperone that binds nickel to bring it safely to its target. The targets for Hypa are the nickel-containing enzymes [Ni,Fe]-hydrogenase and urease. The nickel coordinates with four nitrogens within the protein. The four conserved cysteines towards the C-terminus bind one zinc moiety probably to stabilize the protein fold.


Pssm-ID: 460085  Cd Length: 111  Bit Score: 141.12  E-value: 7.50e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491457357    2 HEMSLAVAAIDLAAEQATQRGFNKVTALWLEVGSFSCVDPDTIAFCFEAAAKGTVVEGAQLHFQHQAAEAWCYDCDKTVT 81
Cdd:pfam01155   1 HELSIAQSIVEIVEEEAKEHGATRVTKVTLEVGELSGVEPEALRFAFEVAAKGTLAEGAELEIEEVPARARCRDCGKEFE 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 491457357   82 LTERGQACPECGGYKLRVAQGDSLRITDIEV 112
Cdd:pfam01155  81 LEERDLVCPRCGSPDVEILSGRELRIKSIEV 111
PRK12380 PRK12380
hydrogenase/urease nickel incorporation protein;
1-112 6.47e-35

hydrogenase/urease nickel incorporation protein;


Pssm-ID: 183485  Cd Length: 113  Bit Score: 115.83  E-value: 6.47e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491457357   1 MHEMSLAVAAIDLAAEQATQRGFNKVTALWLEVGSFSCVDPDTIAFCFEAAAKGTVVEGAQLHFQHQAAEAWCYDCDKTV 80
Cdd:PRK12380   1 MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVV 80

                         90       100       110
                 ....*....|....*....|....*....|..
gi 491457357  81 TLTERGQACPECGGYKLRVAQGDSLRITDIEV 112
Cdd:PRK12380  81 EIHQHDAQCPHCHGERLRVDTGDSLIVKSIEV 112
hypA TIGR00100
hydrogenase nickel insertion protein HypA; CXXC-~12X-CXXC and genetically seems a regulatory ...
 1-113 3.61e-34

hydrogenase nickel insertion protein HypA; CXXC-~12X-CXXC and genetically seems a regulatory protein. In Hpylori, hypA mutant abolished hydrogenase activity and decrease in urease activity. Nickel supplementation in media restored urease activity and partial hydrogenase activity. HypA probably involved in inserting Ni in enzymes. [Protein fate, Protein modification and repair]


Pssm-ID: 272906  Cd Length: 115  Bit Score: 114.01  E-value: 3.61e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491457357    1 MHEMSLAVAAIDLAAEQATQRGFNKVTALWLEVGSFSCVDPDTIAFCFEAAAKGTVVEGAQLHFQHQAAEAWCYDCDKTV 80
Cdd:TIGR00100   1 MHELSLAEAMLEIVEEQAEKHQAKKVTRVTLEIGELSCVNPSQLQFAFEVVREGTVAEGAKLNIEDEPVECECEDCSEEV 80

                          90       100       110
                  ....*....|....*....|....*....|...
gi 491457357   81 TLTERGQACPECGGYKLRVAQGDSLRITDIEVS 113
Cdd:TIGR00100  81 SPEIDLYRCPKCHGIMLQVRAGKELNLKSIEVE 113
hypA PRK03681
hydrogenase/urease nickel incorporation protein HypA;
1-112 4.97e-33

hydrogenase/urease nickel incorporation protein HypA;


Pssm-ID: 179630  Cd Length: 114  Bit Score: 111.41  E-value: 4.97e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491457357   1 MHEMSLAVAAIDLAAEQATQRGFNKVTALWLEVGSFSCVDPDTIAFCFEAAAKGTVVEGAQLHFQHQAAEAWCYDCDKTV 80
Cdd:PRK03681   1 MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSLAFCFDLVCRGTVAEGCKLHLEEQEAECWCETCQQYV 80

                         90       100       110
                 ....*....|....*....|....*....|...
gi 491457357  81 TL-TERGQACPECGGYKLRVAQGDSLRITDIEV 112
Cdd:PRK03681  81 TLlTQRVRRCPQCHGDMLRIVADDGLQIRRIEI 113
hypA PRK00762
hydrogenase maturation nickel metallochaperone HypA;
1-112 9.63e-14

hydrogenase maturation nickel metallochaperone HypA;


Pssm-ID: 179113  Cd Length: 124  Bit Score: 62.44  E-value: 9.63e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491457357   1 MHEMSLAVAAIDLAAEQATQRGFNKVTALWLEVGSFSCVDPDTIAFCFEAAAKGTVVEGAQLHFQHQAAEAWCyDCD--K 78
Cdd:PRK00762   1 MHELSMACEIVEAVIDTAEKNNATEVTEVTLEIGRLTMLNPEQLRFMLDVLAEGTIAEDADLIVEMIPVEIEC-ECGyeG 79

                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 491457357  79 TVTLTERGQ-----ACPECGGYKLRVAQGDSLRITDIEV 112
Cdd:PRK00762  80 VVDEDEIDHyaaviECPVCGNKRAHILGGRECNVKNIKI 118
hypA PRK03824
hydrogenase nickel incorporation protein HypA;
1-112 1.37e-12

hydrogenase nickel incorporation protein HypA;


Pssm-ID: 235164  Cd Length: 135  Bit Score: 59.71  E-value: 1.37e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491457357   1 MHEMSLAVAAIDLAAEQATQRGFNKVTALWLEVGSFSCVDPDTIAFCFEAAAKGTVVEGAQLHFQHQAAEAWCYDCDKTV 80
Cdd:PRK03824   1 MHEWALAEAIVRTVIDYAQKEGASKVKALKVVLGELQDVDKEIVEFALNELLKGTILEGAEIIFEEEEAVLKCRNCGNEW 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 491457357  81 TLTERGQ---------------------ACPECGGYKLRVAQGDSLRITDIEV 112
Cdd:PRK03824  81 SLKEVKEsldeeireaihfipevvhaflKCPKCGSRDFEIVKGRGVYIEEIKI 133
hypA PRK00564
hydrogenase nickel incorporation protein; Provisional
 1-112 1.56e-05

hydrogenase nickel incorporation protein; Provisional


Pssm-ID: 179066  Cd Length: 117  Bit Score: 40.98  E-value: 1.56e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491457357   1 MHEMSLAVAAIDLAAEQATQRGFNKVTALWLEVGSFSCVDPDTIAFCFEA-AAKGTVVEGAQLHFQHQAAEAWCYDCD-- 77
Cdd:PRK00564   1 MHEYSVVSSLIALCEEHAKKNQAHKIEKVVVGIGERSGMDKSLFVSAFETfREESLVCKDAILDIVDEKVELECKDCShv 80

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 491457357  78 ---KTVTLTergqACPECGGYKLRVAQGDSLRITDIEV 112
Cdd:PRK00564  81 fkpNALDYG----VCEKCHSKNVIITQGNEMRLLSLEM 114
zinc_ribbon_9 pfam14369
zinc-ribbon;
72-94 2.57e-05

zinc-ribbon;


Pssm-ID: 433910  Cd Length: 35  Bit Score: 38.35  E-value: 2.57e-05
                          10        20
                  ....*....|....*....|....*.
gi 491457357   72 WCYDCDKTVTLTERGQ---ACPECGG 94
Cdd:pfam14369   4 WCHQCDRFVRIAPSEDsdvACPRCGG 29
rubre_like_arch NF033497
rubrerythrin-like domain; This rubrerythrin-like domain is found primarily in the archaea, ...
 73-94 4.41e-03

rubrerythrin-like domain; This rubrerythrin-like domain is found primarily in the archaea, occasionally as part of a larger redox-active protein. It features two CxxC motifs with a spacer of 12 to 13 amino acids.


Pssm-ID: 411139  Cd Length: 34  Bit Score: 32.62  E-value: 4.41e-03
                         10        20
                 ....*....|....*....|..
gi 491457357  73 CYDCDKTVTLTERGQACPECGG 94
Cdd:NF033497   3 CFDCGTRVTAATHPGTCPDCGG 24
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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