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Conserved domains on  [gi|491602887|ref|WP_005460447|]
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MULTISPECIES: tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL [Vibrio]

Protein Classification

tRNA (cytidine(34)-2'-O)-methyltransferase( domain architecture ID 10000715)

tRNA (cytidine(34)-2'-O)-methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine to the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA)

EC:  2.1.1.207
Gene Ontology:  GO:0003723|GO:0008173|GO:1904047|GO:0141098|GO:0001510
SCOP:  4002340

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-158 6.76e-100

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


:

Pssm-ID: 439989  Cd Length: 153  Bit Score: 283.46  E-value: 6.76e-100
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887   1 MFDIALYEPEIAPNTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRAGLDYHDLARVTRHKNYQAFLeyldkEKQGNYRL 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFL-----EALPGGRL 75

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 491602887  81 FACTTKTTGHHVDAQYQAGDVLMFGPETRGLPAEIIEGlPMEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQMGFEGA 158
Cdd:COG0219   76 FLFTTKGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAA-PADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
 
Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-158 6.76e-100

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 283.46  E-value: 6.76e-100
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887   1 MFDIALYEPEIAPNTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRAGLDYHDLARVTRHKNYQAFLeyldkEKQGNYRL 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFL-----EALPGGRL 75

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 491602887  81 FACTTKTTGHHVDAQYQAGDVLMFGPETRGLPAEIIEGlPMEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQMGFEGA 158
Cdd:COG0219   76 FLFTTKGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAA-PADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 4-153 5.95e-85

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 245.44  E-value: 5.95e-85
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887   4 IALYEPEIAPNTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRAGLDYHDLARVTRHKNYQAFLEYLDkekqgNYRLFAC 83
Cdd:cd18094    2 IVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALP-----PGRLFLF 76

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887  84 TTKTTGHHVDAQYQAGDVLMFGPETRGLPAEIIEGLPmEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQM 153
Cdd:cd18094   77 TTKGTKPYTDFDFKPGDYLVFGRESTGLPEEILDAFP-DRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-159 5.48e-82

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 238.74  E-value: 5.48e-82
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887   1 MFDIALYEPEIAPNTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRAGLDYHDLARVTRHKNYQAFLEYLDKEkqgnyRL 80
Cdd:PRK10358   1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQ-----RL 75

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 491602887  81 FACTTKTTGHHVDAQYQAGDVLMFGPETRGLPAEIIEGLPMEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQMGFEGAL 159
Cdd:PRK10358  76 FALTTKGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAV 154
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 1-158 2.13e-71

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 211.51  E-value: 2.13e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887    1 MFDIALYEPEIAPNTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRAGLDYHDLARVTRHKNYQAFLEYLDKEKqgnyrL 80
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEKPQK-----L 75

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 491602887   81 FACTTKTTGHHVDAQYQAGDVLMFGPETRGLPAEIIEGLpMEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQMGFEGA 158
Cdd:TIGR00185  76 FALTKKGTPAHSAVTYQDGDYLMFGPETRGLPQSILDNL-MEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGYPGL 152
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 4-147 2.16e-44

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 142.67  E-value: 2.16e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887    4 IALYEPEIAPNTGNIIRLCANCGAN-LHLIEPLGFDLEEKKVRRAGLDYHDLARVTRHKNYQAFLEYLDKEkqgNYRLFA 82
Cdd:pfam00588   4 VVLDNIQDPGNLGAIIRTAAAFGADgLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEA---GYAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 491602887   83 CTTKTTgHHVDAQYQAGDVLMFGPETRGLPAEIIEGLPmeQRIRIPMMPDARSLNLSNAVAIIAF 147
Cdd:pfam00588  81 TSLGGK-SLYEIDFPKKVALVFGNEGTGLSNEVLELCD--RLVRIPMSGDVESLNVAVAAAIALY 142
 
Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-158 6.76e-100

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 283.46  E-value: 6.76e-100
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887   1 MFDIALYEPEIAPNTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRAGLDYHDLARVTRHKNYQAFLeyldkEKQGNYRL 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFL-----EALPGGRL 75

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 491602887  81 FACTTKTTGHHVDAQYQAGDVLMFGPETRGLPAEIIEGlPMEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQMGFEGA 158
Cdd:COG0219   76 FLFTTKGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAA-PADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 4-153 5.95e-85

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 245.44  E-value: 5.95e-85
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887   4 IALYEPEIAPNTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRAGLDYHDLARVTRHKNYQAFLEYLDkekqgNYRLFAC 83
Cdd:cd18094    2 IVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALP-----PGRLFLF 76

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887  84 TTKTTGHHVDAQYQAGDVLMFGPETRGLPAEIIEGLPmEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQM 153
Cdd:cd18094   77 TTKGTKPYTDFDFKPGDYLVFGRESTGLPEEILDAFP-DRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-159 5.48e-82

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 238.74  E-value: 5.48e-82
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887   1 MFDIALYEPEIAPNTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRAGLDYHDLARVTRHKNYQAFLEYLDKEkqgnyRL 80
Cdd:PRK10358   1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQ-----RL 75

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 491602887  81 FACTTKTTGHHVDAQYQAGDVLMFGPETRGLPAEIIEGLPMEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQMGFEGAL 159
Cdd:PRK10358  76 FALTTKGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAV 154
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 1-158 2.13e-71

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 211.51  E-value: 2.13e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887    1 MFDIALYEPEIAPNTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRAGLDYHDLARVTRHKNYQAFLEYLDKEKqgnyrL 80
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEKPQK-----L 75

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 491602887   81 FACTTKTTGHHVDAQYQAGDVLMFGPETRGLPAEIIEGLpMEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQMGFEGA 158
Cdd:TIGR00185  76 FALTKKGTPAHSAVTYQDGDYLMFGPETRGLPQSILDNL-MEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGYPGL 152
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 4-147 2.16e-44

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 142.67  E-value: 2.16e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887    4 IALYEPEIAPNTGNIIRLCANCGAN-LHLIEPLGFDLEEKKVRRAGLDYHDLARVTRHKNYQAFLEYLDKEkqgNYRLFA 82
Cdd:pfam00588   4 VVLDNIQDPGNLGAIIRTAAAFGADgLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEA---GYAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 491602887   83 CTTKTTgHHVDAQYQAGDVLMFGPETRGLPAEIIEGLPmeQRIRIPMMPDARSLNLSNAVAIIAF 147
Cdd:pfam00588  81 TSLGGK-SLYEIDFPKKVALVFGNEGTGLSNEVLELCD--RLVRIPMSGDVESLNVAVAAAIALY 142
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 4-149 2.74e-32

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 111.93  E-value: 2.74e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887   4 IALYEPEIAPNTGNIIRLCANCGA-NLHLIEPLGfDLEEKKVRRAGLDYHDLARVTRHKNyqaFLEYLDKEKQGNYRLFA 82
Cdd:cd18082    2 LVLDNIRSPGNIGSIFRTADAFGVeKIYLVGPTA-DIFDPKAKRTSRGTHFSVPYERVDN---FEEYLKSLKEEGYPLIG 77

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887  83 CTTKTTG---HHVDAQYQAGDVLMFGPETRGLPAEIIEGlpMEQRIRIPMMPDARSLNLSNAVAIIAFEA 149
Cdd:cd18082   78 LELTDGSiplSEFKFKKPKKIALIFGNERSGLSPELLAL--CDYTVHIPMSGKNSSLNVSVATGIALYEF 145
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 14-152 1.40e-14

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 68.68  E-value: 1.40e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887  14 NTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRA---GLDYHDLARVTrhkNYQAFLEYLdkeKQGNYRLFACTTKTTGH 90
Cdd:COG0566  128 NLGAILRSAAAFGVDGVILPKRRADPYNPKVVRAsagALFHVPVARVT---NLADALEEL---KEAGFWVVGTDLDGEAS 201

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 491602887  91 HVDAQYQAGDVLMFGPETRGLPAEIIEGLpmEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQ 152
Cdd:COG0566  202 LYEVDLPGPVALVLGNEGKGLSRLVLEAC--DELVRIPMAGGVESLNVAVAAAILLYEAARQ 261
SpoU-like_RNA-MTase cd18104
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 74-152 1.65e-14

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349977  Cd Length: 146  Bit Score: 66.45  E-value: 1.65e-14
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 491602887  74 KQGNYRLFACTTKTTGHHVDAQYQAGDVLMFGPETRGLPAEIIEGLpmEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQ 152
Cdd:cd18104   69 KENKIRILATTPKAEKLYYEADLRGPIAIVVGSESEGLSEFWLKAA--DERVRIPMAGQADSLNVSVAAAILLYEALRQ 145
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 4-148 2.43e-13

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 63.28  E-value: 2.43e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887   4 IALYEPEIAPNTGNIIRLCANCGA-NLHLIEPLGFDLEEKKVRRAGLDYHDLARVTRHKNYQAFLEYLDkekqgnyRLFA 82
Cdd:cd18093    3 VVLVEPQHPGNIGAVARAMKNFGLsDLRLVNPRCDDPEEAARALASGAEDVLENAKVYDTLEEALADCD-------LVVA 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887  83 CTTKTTGHH------------VDAQYQAGDV-LMFGPETRGLPAEIIEglpMEQRI-RIPMMPDARSLNLSNAVAIIAFE 148
Cdd:cd18093   76 TTARPRGLRrplltprelaelLKEHAAGGKVaLVFGREDSGLTNEELA---LCDLLvTIPTNPEYPSLNLAQAVAIVLYE 152
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 14-153 9.27e-13

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 62.10  E-value: 9.27e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887  14 NTGNIIRLCANCG-ANLHLIEPlgfDLEEKKVRRAGLDYHDLARVTRHKNYQAFLEYLdkeKQGNYRLFActtkTTGHHV 92
Cdd:cd18092   29 NASAVLRTCDAFGvQDVHVIEP---KNKFKKNRGVAMGADKWLDVHRHDSTEECLAAL---KEQGYRIVA----TSLSPD 98

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 491602887  93 DAQYQAGDV-----LMFGPETRGLPAEIIEGlpMEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQM 153
Cdd:cd18092   99 AVPLDELDYtrptaLVFGNEKDGVSEEALAA--ADERVYIPMYGFVESLNVSVAAAIILYELQRQR 162
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 14-149 1.01e-11

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 58.98  E-value: 1.01e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887  14 NTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRAGLDYHDLARVTRHKNYQAFLEYLdkeKQGNYRLFACTTKTTGHHVD 93
Cdd:cd18095   13 NLGTIIRTAAAFGVDGVILSKGTVDPYNPKVVRASMGALFRLPIIRVDDLEELLEEL---KEAGYKVVAADLDGGSSLYD 89

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 491602887  94 AQYQAGDVLMFGPETRGLPAEIIEGLpmEQRIRIPMMPDARSLNLSNAVAIIAFEA 149
Cdd:cd18095   90 LDLPKPVALVLGNEGNGLSDELLELC--DKRVTIPMSGGVESLNVAVAAAILLYEL 143
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 4-154 2.42e-10

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 56.66  E-value: 2.42e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887   4 IALYEPEIAPNTGNIIRLCANCG-ANLHLIEPLGFdLEEKKVRRA--GLDYhdLARVTRHKNYQAFLEyldkekqGNYRL 80
Cdd:COG0565    6 VVLVEPQHPGNIGAAARAMKNFGlSDLRLVNPRGW-PDEEARAMAsgADDV--LENARVVDTLEEALA-------DCDLV 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887  81 FAcTTKTTGH-------------HVDAQYQAGDV-LMFGPETRGLPAEIIEglpMEQRI-RIPMMPDARSLNLSNAVAII 145
Cdd:COG0565   76 VG-TTARPRElrkpvltpreaaeELLARAAGGKVaLVFGRERSGLTNEELA---LCDALvTIPTNPEYPSLNLAQAVQVV 151


                 ....*....
gi 491602887 146 AFEAWRQMG 154
Cdd:COG0565  152 AYELRKAAL 160
SpoU-like_RlmB cd18103
SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the ...
 14-149 8.70e-08

SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349976  Cd Length: 143  Bit Score: 48.53  E-value: 8.70e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887  14 NTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRA---GLDYHDLARVTrhkNYQAFLEYLdkeKQGNYRLFACTTKTTGH 90
Cdd:cd18103   13 NLGAIIRSAEAFGVDGVIIPKRRSAPLTPTVAKAsagALEHLPIARVT---NLARALEEL---KEAGFWIYGADGEAEKS 86

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 491602887  91 HVDAQYQAGDVLMFGPETRGLPAEIIEGLpmEQRIRIPMMPDARSLNLSNAVAIIAFEA 149
Cdd:cd18103   87 LYDVDLTGPLALVLGSEGKGLRRLVLKNC--DFLVKIPMAGKVDSLNVSVAAGIILYEI 143
rRNA_methyl_3 TIGR00186
rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases ...
 47-152 1.22e-06

rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 129290 [Multi-domain]  Cd Length: 237  Bit Score: 46.57  E-value: 1.22e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887   47 AGLDYH-DLARVTRhknyqaFLEYLDKEKQGNYRLFActtkTTGHHVDAQYQAGD----VLMFGPETRGLPAEIIEGlpM 121
Cdd:TIGR00186 138 SGAVEYvPLARVTN------LSRTITKLKESGFWTVG----TDLDAQDTLYQVKLtkplALVVGNEGEGVSRLIKEN--C 205

                          90       100       110
                  ....*....|....*....|....*....|.
gi 491602887  122 EQRIRIPMMPDARSLNLSNAVAIIAFEAWRQ 152
Cdd:TIGR00186 206 DFLIKIPMAGKVDSLNVSVAAGILLFEIKRQ 236
SpoU-like_RNMTL1 cd18106
SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) ...
 14-149 1.78e-06

SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) catalyzes the methylation of 2'-O-ribose residues G1370 to GmG residue of the mitochondrial 16S rRNA. RNMTL1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349979  Cd Length: 148  Bit Score: 44.93  E-value: 1.78e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887  14 NTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRAGLDYHdlarvTRHKNYQAfleyLDKEKQGNY-----RLFACTTKTT 88
Cdd:cd18106   13 NLGSILRSAAAAGCSKVLLTKGCVDPWEPKVLRAGAGAH-----FRLPIYGN----LSWDDIPNYlpddsKVFLADNNLP 83

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 491602887  89 GHHVDA-QYQAGD-VLMFGPETRGLPAEIIEGLPME--QRIRIPMMPDARSLNLSNAVAIIAFEA 149
Cdd:cd18106   84 VQPYDSiDYCSGPiVLVIGGETHGVSPEAYKLAHSTggSRVHIPLANGVDSLNSAVAASILLFEM 148
SpoU-like cd18097
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 14-149 2.39e-06

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349970  Cd Length: 144  Bit Score: 44.48  E-value: 2.39e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887  14 NTGNIIRLCANCGAN-LHL--IEPLGfdlEEKKVRRAGLDYHDLARVTRHKNYQAFLEYLdkeKQGNYRLFActTKTTGH 90
Cdd:cd18097   13 NVGSIFRTADAAGVEkIYLcgYTPTP---PHPKVAKTALGAEESVPWEHFEDTLEAVRAL---KARGYTIIA--LETTEN 84

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 491602887  91 HVD---AQYQAGDVLMFGPETRGLPAEIIEGLpmEQRIRIPMMPDARSLNLSNAVAIIAFEA 149
Cdd:cd18097   85 SVPlheARLPRPLALVLGNEVRGVSPEVLALC--DAVVEIPMFGRKNSLNVAVAAAVALYEL 144
SpoU-like_AviRb cd18107
SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes ...
 14-149 4.06e-06

SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA. AviRb is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349980  Cd Length: 148  Bit Score: 43.92  E-value: 4.06e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887  14 NTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRA--GLDYHDLA-RVTRHKNYQAFLEYLdKEKQGNYRLFActtktTGH 90
Cdd:cd18107   13 NLGTIIRSADALGAHGLVVTGHAADVYDPKTIRAstGSLFSLPIvRVPSNRELEDWVEAR-KARGTPLQLVG-----TDE 86

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 491602887  91 HVDAQYQAGD-----VLMFGPETRGLPA---EIIEGLpmeqrIRIPMMPDARSLNLSNAVAIIAFEA 149
Cdd:cd18107   87 HGDHQIADHDftrptLLLIGNETAGLSAawrELCDET-----VSIPMAGSASSLNAACAASIFLYEA 148
SpoU-like_RNA-MTase cd18109
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 14-148 5.47e-05

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349982  Cd Length: 141  Bit Score: 40.60  E-value: 5.47e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887  14 NTGNIIRLCANCGANLHLIEPLGFDLEEKKVRRA--GldyhDLARVTRHknYQAFLEYLdKEKQGNYRLFActTKTTGHH 91
Cdd:cd18109   13 NLGTIIRTADWFGIRQIICSPDTVDPYNPKVVQAsmG----SIFRVQIH--YTDLPALL-AELKKAFPVYG--ADLDGEN 83

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 491602887  92 V-DAQYQAGDVLMFGPETRGLPAEIIEGLpmEQRIRIPMMPD-ARSLNLSNAVAIIAFE 148
Cdd:cd18109   84 LyEFTLPEKGILVMGNEGHGISPEVLKLL--THRLTIPRYGKgAESLNVAVATAILLSE 140
PRK11081 PRK11081
tRNA guanosine-2'-O-methyltransferase; Provisional
 101-152 8.07e-04

tRNA guanosine-2'-O-methyltransferase; Provisional


Pssm-ID: 236837 [Multi-domain]  Cd Length: 229  Bit Score: 38.43  E-value: 8.07e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 491602887 101 VLMfGPETRGLPAEIIEglPMEQRIRIPMMPDARSLNLSNAVAIIAFEAWRQ 152
Cdd:PRK11081 116 ILM-GQEKTGISQEALA--LADQDIIIPMIGMVQSLNVSVASALILYEAQRQ 164
rRNA_methyl_1 TIGR00050
RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of ...
 1-148 8.23e-04

RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases, and is now characterized, in E. coli, as a tRNA:Cm32/Um32 methyltransferase. It may be named TrMet(Xm32), or TrmJ, according to the nomenclature style chosen [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272876 [Multi-domain]  Cd Length: 233  Bit Score: 38.15  E-value: 8.23e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887    1 MFDIALYEPEIAPNTGNIIRLCANCG-ANLHLIEPLGFDLEEKKVRRAGL-DYHDLARVtrhknYQAFLEYLDKekqgny 78
Cdd:TIGR00050   3 NIRIVLVEPSHPGNIGSIARAMKNMGlTELRLVNPRVKLEEEAYALAAGArDILDNAKV-----VDTLDEALDD------ 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887   79 RLFACTTKTTGHHVDAQY----QAGDV---------LMFGPETRGLPAEIIEGLPMeqRIRIPMMPDARSLNLSNAVAII 145
Cdd:TIGR00050  72 CDFVVGTSARSRNLQRPMltprELAPKlvaekgkvaLVFGREDSGLTNEELQKCHV--LVSIPTSEEYPSLNLSHAVAVI 149


                  ...
gi 491602887  146 AFE 148
Cdd:TIGR00050 150 LYE 152
SpoU-like_TRM3-like cd18091
SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O) ...
 13-148 6.17e-03

SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O)-methyltransferase TRM3 catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in various tRNAs. TRM3 is similar to C-terminal domain of TAR (HIV-1) RNA binding protein 1 (TARBP1), a protein binding to TAR, which functions as a RNA regulatory signal by forming a stable stem-loop structure to which transactivator protein Tat binds. The role of TARBP1 is believed to be to disengage RNA polymerase II from TAR during transcriptional elongation. TRM3 and the C-terminal methyltransferase domain of TARBP1 are members of the SPOUT methyltransferase superfamily.


Pssm-ID: 349964  Cd Length: 145  Bit Score: 35.20  E-value: 6.17e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 491602887  13 PNTGNIIRLCANCGANLHLIEPLGFdLEEKKVRRAGLDYHDLARVTRHKNYQaFLEYLDKEKQGNYRLFAC--TTKTTGH 90
Cdd:cd18091   12 PNLGGLCRTCEIFGVSKLVLPNLAV-VEDKQFKALSVSAEKWLPIEEVKPED-LLPYLKEKKQEGYTIVGLeqTANSVSL 89

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 491602887  91 HvDAQYQAGDVLMFGPETRGLPAEIIEglPMEQRIRIPMMPDARSLNLSNAVAIIAFE 148
Cdd:cd18091   90 H-DFQFPKKTVLVLGNEKEGIPAELLS--LLDVCVEIPQLGVIRSLNVHVSGAIAIYE 144
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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