NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|495713280|ref|WP_008437859|]
View 

MULTISPECIES: type VII toxin-antitoxin system HepT family RNase toxin [unclassified Pseudomonas]

Protein Classification

DUF86 domain-containing protein( domain architecture ID 10006451)

uncharacterized DUF86 domain-containing protein similar to Bacillus subtilis YutE

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
YutE COG2445
Uncharacterized HEPN domain protein YutE, UPF0331/DUF86 family [General function prediction ...
 3-133 5.57e-48

Uncharacterized HEPN domain protein YutE, UPF0331/DUF86 family [General function prediction only];


:

Pssm-ID: 441985  Cd Length: 132  Bit Score: 150.82  E-value: 5.57e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495713280   3 DDVLINKAASIERCVARARQEYDKDPTSFATDFTRQDAAIFNIQRACEAALDMGQHLIRRERLGVPQGARDVFELLCQGG 82
Cdd:COG2445    1 RERIEEKLEYIEEYLARLEELAELSPEEFLSDPTEQDAVERNLQLAIEAAIDIGNHIIAGFGLRDPGSYRDIFDILAEEG 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 495713280  83 WVNPALLTNLKNMVGFRNIAVHEYQTLQLPITVAIITKHLDEFLAFSSHIL 133
Cdd:COG2445   81 VISEELAEKLKKMVGFRNILVHDYLEIDLEEVYEILKEHLDDLEEFIKEIR 131
 
Name Accession Description Interval E-value
YutE COG2445
Uncharacterized HEPN domain protein YutE, UPF0331/DUF86 family [General function prediction ...
 3-133 5.57e-48

Uncharacterized HEPN domain protein YutE, UPF0331/DUF86 family [General function prediction only];


Pssm-ID: 441985  Cd Length: 132  Bit Score: 150.82  E-value: 5.57e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495713280   3 DDVLINKAASIERCVARARQEYDKDPTSFATDFTRQDAAIFNIQRACEAALDMGQHLIRRERLGVPQGARDVFELLCQGG 82
Cdd:COG2445    1 RERIEEKLEYIEEYLARLEELAELSPEEFLSDPTEQDAVERNLQLAIEAAIDIGNHIIAGFGLRDPGSYRDIFDILAEEG 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 495713280  83 WVNPALLTNLKNMVGFRNIAVHEYQTLQLPITVAIITKHLDEFLAFSSHIL 133
Cdd:COG2445   81 VISEELAEKLKKMVGFRNILVHDYLEIDLEEVYEILKEHLDDLEEFIKEIR 131
HepT-like pfam01934
Ribonuclease HepT-like; This family includes the toxic component HepT of a type II ...
 13-132 2.80e-27

Ribonuclease HepT-like; This family includes the toxic component HepT of a type II toxin-antitoxin (TA) system, which has RNase activity. These proteins contain a HEPN (higher eukaryotes and prokaryotes nucleotide-binding) domain and are neutralized through tri-AMPylation by the cognate antitoxin MntA, containing a MNT (minimal nucleotidyltransferase) domain. HepT-MnA form an heterooctamer (at a 2:6 ratio), a rare organization for this kind of TA systems. HepT dimerizes and enables the formation of a deep cleft at the HEPN-domain interface, containing the RX4-6H motif (where X is any amino acid and the residue immediately after the conserved R is typically a polar amino acid) as the active site that functions as an RNA-cleaving RNase. This type II TA system regulates cell motility and confers plasmid stability. Due to the prevalence of these HEPN/MNT modules in bacteria and archaea, it has been suggested that these TA systems may also play a role in the environmental adaptation to extreme habitats.


Pssm-ID: 460390  Cd Length: 120  Bit Score: 98.19  E-value: 2.80e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495713280   13 IERCVARARQEY-DKDPTSFATDFTRQDAAIFNIQRACEAALDMGQHLIRRERLGVPqGARDVFELLCQGGWVNPALLTN 91
Cdd:pfam01934   1 ILEAIERIEEFTqGLTEEEFLSDRLTQDAVERNLEIIGEAAIDIGNHLISDLGLRDP-GSYDIFDILAEEGVISEEPAIP 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 495713280   92 LKNMVGFRNIAVHEYQTLQLPITVAIITKHLDEFLAFSSHI 132
Cdd:pfam01934  80 WRKMAGLRNILVHGYFSIDLEIVWEIIQEDLPDLEEFLEKI 120
 
Name Accession Description Interval E-value
YutE COG2445
Uncharacterized HEPN domain protein YutE, UPF0331/DUF86 family [General function prediction ...
 3-133 5.57e-48

Uncharacterized HEPN domain protein YutE, UPF0331/DUF86 family [General function prediction only];


Pssm-ID: 441985  Cd Length: 132  Bit Score: 150.82  E-value: 5.57e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495713280   3 DDVLINKAASIERCVARARQEYDKDPTSFATDFTRQDAAIFNIQRACEAALDMGQHLIRRERLGVPQGARDVFELLCQGG 82
Cdd:COG2445    1 RERIEEKLEYIEEYLARLEELAELSPEEFLSDPTEQDAVERNLQLAIEAAIDIGNHIIAGFGLRDPGSYRDIFDILAEEG 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 495713280  83 WVNPALLTNLKNMVGFRNIAVHEYQTLQLPITVAIITKHLDEFLAFSSHIL 133
Cdd:COG2445   81 VISEELAEKLKKMVGFRNILVHDYLEIDLEEVYEILKEHLDDLEEFIKEIR 131
HepT-like pfam01934
Ribonuclease HepT-like; This family includes the toxic component HepT of a type II ...
 13-132 2.80e-27

Ribonuclease HepT-like; This family includes the toxic component HepT of a type II toxin-antitoxin (TA) system, which has RNase activity. These proteins contain a HEPN (higher eukaryotes and prokaryotes nucleotide-binding) domain and are neutralized through tri-AMPylation by the cognate antitoxin MntA, containing a MNT (minimal nucleotidyltransferase) domain. HepT-MnA form an heterooctamer (at a 2:6 ratio), a rare organization for this kind of TA systems. HepT dimerizes and enables the formation of a deep cleft at the HEPN-domain interface, containing the RX4-6H motif (where X is any amino acid and the residue immediately after the conserved R is typically a polar amino acid) as the active site that functions as an RNA-cleaving RNase. This type II TA system regulates cell motility and confers plasmid stability. Due to the prevalence of these HEPN/MNT modules in bacteria and archaea, it has been suggested that these TA systems may also play a role in the environmental adaptation to extreme habitats.


Pssm-ID: 460390  Cd Length: 120  Bit Score: 98.19  E-value: 2.80e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495713280   13 IERCVARARQEY-DKDPTSFATDFTRQDAAIFNIQRACEAALDMGQHLIRRERLGVPqGARDVFELLCQGGWVNPALLTN 91
Cdd:pfam01934   1 ILEAIERIEEFTqGLTEEEFLSDRLTQDAVERNLEIIGEAAIDIGNHLISDLGLRDP-GSYDIFDILAEEGVISEEPAIP 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 495713280   92 LKNMVGFRNIAVHEYQTLQLPITVAIITKHLDEFLAFSSHI 132
Cdd:pfam01934  80 WRKMAGLRNILVHGYFSIDLEIVWEIIQEDLPDLEEFLEKI 120
HEPN COG2361
HEPN domain protein, predicted toxin of MNT-HEPN system [Defense mechanisms];
12-133 3.54e-05

HEPN domain protein, predicted toxin of MNT-HEPN system [Defense mechanisms];


Pssm-ID: 441928  Cd Length: 115  Bit Score: 40.63  E-value: 3.54e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495713280  12 SIERCVARARqeyDKDPTSFATDFTRQDAAIFNIQRACEAAldmgqhliRRerlgVPQGARDVfellcqggwvNPALltN 91
Cdd:COG2361   16 AIERIEEYTE---GLDFEDFLEDELTQDAVIRNLEIIGEAA--------KR----IPDEFREK----------HPEI--P 68

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 495713280  92 LKNMVGFRNIAVHEYQTLQLPITVAIITKHLDEFLAFSSHIL 133
Cdd:COG2361   69 WRQIAGMRNRLAHGYFGVDLEIVWDTVQNDLPELKEQIEAIL 110
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH