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Conserved domains on  [gi|496082344|ref|WP_008806851|]
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MULTISPECIES: glycosyltransferase family 2 protein [Klebsiella]

Protein Classification

glycosyltransferase family 2 protein( domain architecture ID 10118426)

glycosyltransferase family 2 protein catalyzes the transfer of saccharide moieties from a donor to an acceptor to form glycosidic bonds

CATH:  3.90.550.10
CAZY:  GT2
EC:  2.4.-.-
Gene Ontology:  GO:0016757|GO:0006486
PubMed:  9445404|12691742|10508766|12200473|10521532
SCOP:  3000077

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Beta4Glucosyltransferase cd02511
UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of ...
 3-226 1.99e-65

UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of lipooligosaccharide; UDP-glucose: lipooligosaccharide (LOS) beta-1-4-glucosyltransferase catalyzes the addition of the first residue, glucose, of the lacto-N-neotetrase structure to HepI of the LOS inner core. LOS is the major constituent of the outer leaflet of the outer membrane of gram-positive bacteria. It consists of a short oligosaccharide chain of variable composition (alpha chain) attached to a branched inner core which is lined in turn to lipid A. Beta 1,4 glucosyltransferase is required to attach the alpha chain to the inner core.


:

Pssm-ID: 133005 [Multi-domain]  Cd Length: 229  Bit Score: 203.29  E-value: 1.99e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344   3 KLSVCLLTCNSARLLMEVLPPLLKVADECIIVDSGSTDETVSICQQFGLTVHHHAYKAHGAQMNYAIGLASHDWVLCMDS 82
Cdd:cd02511    1 TLSVVIITKNEERNIERCLESVKWAVDEIIVVDSGSTDRTVEIAKEYGAKVYQRWWDGFGAQRNFALELATNDWVLSLDA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344  83 DEILDNDVVAAIQALKAGEEPDptcAWRLPRYWFVLGTQVRtiYPISSPDYPVRLFNRQQARFNDRPVDDQVVGHA-RSV 161
Cdd:cd02511   81 DERLTPELADEILALLATDDYD---GYYVPRRNFFLGRWIR--HGGWYPDRQLRLFRRGKARFEDGRVHEQVVVDGgVGI 155

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344 162 RLPGFVRHDTFYSLHEVFNKLNSYTTRLVKYQQIK---PSLTRGIVSA--IGAFFKWYLFSGAWRYGKVG 226
Cdd:cd02511  156 VLKGDILHYGYKSLEEFLEKHNRYSSLEAKDLAAKgkkRSLLKGLLLGrpLLAFLKMYILKRGFLDGRAG 225
 
Name Accession Description Interval E-value
Beta4Glucosyltransferase cd02511
UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of ...
 3-226 1.99e-65

UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of lipooligosaccharide; UDP-glucose: lipooligosaccharide (LOS) beta-1-4-glucosyltransferase catalyzes the addition of the first residue, glucose, of the lacto-N-neotetrase structure to HepI of the LOS inner core. LOS is the major constituent of the outer leaflet of the outer membrane of gram-positive bacteria. It consists of a short oligosaccharide chain of variable composition (alpha chain) attached to a branched inner core which is lined in turn to lipid A. Beta 1,4 glucosyltransferase is required to attach the alpha chain to the inner core.


Pssm-ID: 133005 [Multi-domain]  Cd Length: 229  Bit Score: 203.29  E-value: 1.99e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344   3 KLSVCLLTCNSARLLMEVLPPLLKVADECIIVDSGSTDETVSICQQFGLTVHHHAYKAHGAQMNYAIGLASHDWVLCMDS 82
Cdd:cd02511    1 TLSVVIITKNEERNIERCLESVKWAVDEIIVVDSGSTDRTVEIAKEYGAKVYQRWWDGFGAQRNFALELATNDWVLSLDA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344  83 DEILDNDVVAAIQALKAGEEPDptcAWRLPRYWFVLGTQVRtiYPISSPDYPVRLFNRQQARFNDRPVDDQVVGHA-RSV 161
Cdd:cd02511   81 DERLTPELADEILALLATDDYD---GYYVPRRNFFLGRWIR--HGGWYPDRQLRLFRRGKARFEDGRVHEQVVVDGgVGI 155

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344 162 RLPGFVRHDTFYSLHEVFNKLNSYTTRLVKYQQIK---PSLTRGIVSA--IGAFFKWYLFSGAWRYGKVG 226
Cdd:cd02511  156 VLKGDILHYGYKSLEEFLEKHNRYSSLEAKDLAAKgkkRSLLKGLLLGrpLLAFLKMYILKRGFLDGRAG 225
WcaA COG0463
Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis]; ...
 1-177 5.56e-21

Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440231 [Multi-domain]  Cd Length: 208  Bit Score: 87.84  E-value: 5.56e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344   1 MLKLSVCLLTCNSARLLMEVLPPLLKVAD---ECIIVDSGSTDETVSICQQFG-----LTVHHHAYKA-HGAQMNYAIGL 71
Cdd:COG0463    1 MPLVSVVIPTYNEEEYLEEALESLLAQTYpdfEIIVVDDGSTDGTAEILRELAakdprIRVIRLERNRgKGAARNAGLAA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344  72 ASHDWVLCMDSDEILDNDVVAAIQALKAGEEPDPTCAWR--------LPRYWFVLGTQVRTIYPISSPDYPVRLFNRQ-- 141
Cdd:COG0463   81 ARGDYIAFLDADDQLDPEKLEELVAALEEGPADLVYGSRliregesdLRRLGSRLFNLVRLLTNLPDSTSGFRLFRREvl 160

                        170       180       190
                 ....*....|....*....|....*....|....*..
gi 496082344 142 -QARFNDRPVDDQVVGHARSVRLPgFVRHDTFYSLHE 177
Cdd:COG0463  161 eELGFDEGFLEDTELLRALRHGFR-IAEVPVRYRAGE 196
Glycos_transf_2 pfam00535
Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, ...
 5-142 3.35e-12

Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.


Pssm-ID: 425738 [Multi-domain]  Cd Length: 166  Bit Score: 62.80  E-value: 3.35e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344    5 SVCLLTCNSARLLMEVLPPLLKV---ADECIIVDSGSTDETVSICQQF-----GLTVHHHAYK-AHGAQMNYAIGLASHD 75
Cdd:pfam00535   1 SVIIPTYNEEKYLLETLESLLNQtypNFEIIVVDDGSTDGTVEIAEEYakkdpRVRVIRLPENrGKAGARNAGLRAATGD 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 496082344   76 WVLCMDSDEILDNDVVAAIQALKAGEEPDPTCAWRLPRYWFVLGTQVRTIYPISSPDYPVRLFNRQQ 142
Cdd:pfam00535  81 YIAFLDADDEVPPDWLEKLVEALEEDGADVVVGSRYVIFGETGEYRRASRITLSRLPFFLGLRLLGL 147
PRK13915 PRK13915
putative glucosyl-3-phosphoglycerate synthase; Provisional
 1-55 4.49e-04

putative glucosyl-3-phosphoglycerate synthase; Provisional


Pssm-ID: 237556 [Multi-domain]  Cd Length: 306  Bit Score: 40.67  E-value: 4.49e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 496082344   1 MLKLSVCLLTCNSA-----------RLLMEVLPpllkvaDECIIVDSGSTDETVSICQQFGLTVHH 55
Cdd:PRK13915  30 GRTVSVVLPALNEEetvgkvvdsirPLLMEPLV------DELIVIDSGSTDATAERAAAAGARVVS 89
 
Name Accession Description Interval E-value
Beta4Glucosyltransferase cd02511
UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of ...
 3-226 1.99e-65

UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of lipooligosaccharide; UDP-glucose: lipooligosaccharide (LOS) beta-1-4-glucosyltransferase catalyzes the addition of the first residue, glucose, of the lacto-N-neotetrase structure to HepI of the LOS inner core. LOS is the major constituent of the outer leaflet of the outer membrane of gram-positive bacteria. It consists of a short oligosaccharide chain of variable composition (alpha chain) attached to a branched inner core which is lined in turn to lipid A. Beta 1,4 glucosyltransferase is required to attach the alpha chain to the inner core.


Pssm-ID: 133005 [Multi-domain]  Cd Length: 229  Bit Score: 203.29  E-value: 1.99e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344   3 KLSVCLLTCNSARLLMEVLPPLLKVADECIIVDSGSTDETVSICQQFGLTVHHHAYKAHGAQMNYAIGLASHDWVLCMDS 82
Cdd:cd02511    1 TLSVVIITKNEERNIERCLESVKWAVDEIIVVDSGSTDRTVEIAKEYGAKVYQRWWDGFGAQRNFALELATNDWVLSLDA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344  83 DEILDNDVVAAIQALKAGEEPDptcAWRLPRYWFVLGTQVRtiYPISSPDYPVRLFNRQQARFNDRPVDDQVVGHA-RSV 161
Cdd:cd02511   81 DERLTPELADEILALLATDDYD---GYYVPRRNFFLGRWIR--HGGWYPDRQLRLFRRGKARFEDGRVHEQVVVDGgVGI 155

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344 162 RLPGFVRHDTFYSLHEVFNKLNSYTTRLVKYQQIK---PSLTRGIVSA--IGAFFKWYLFSGAWRYGKVG 226
Cdd:cd02511  156 VLKGDILHYGYKSLEEFLEKHNRYSSLEAKDLAAKgkkRSLLKGLLLGrpLLAFLKMYILKRGFLDGRAG 225
WcaA COG0463
Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis]; ...
 1-177 5.56e-21

Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440231 [Multi-domain]  Cd Length: 208  Bit Score: 87.84  E-value: 5.56e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344   1 MLKLSVCLLTCNSARLLMEVLPPLLKVAD---ECIIVDSGSTDETVSICQQFG-----LTVHHHAYKA-HGAQMNYAIGL 71
Cdd:COG0463    1 MPLVSVVIPTYNEEEYLEEALESLLAQTYpdfEIIVVDDGSTDGTAEILRELAakdprIRVIRLERNRgKGAARNAGLAA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344  72 ASHDWVLCMDSDEILDNDVVAAIQALKAGEEPDPTCAWR--------LPRYWFVLGTQVRTIYPISSPDYPVRLFNRQ-- 141
Cdd:COG0463   81 ARGDYIAFLDADDQLDPEKLEELVAALEEGPADLVYGSRliregesdLRRLGSRLFNLVRLLTNLPDSTSGFRLFRREvl 160

                        170       180       190
                 ....*....|....*....|....*....|....*..
gi 496082344 142 -QARFNDRPVDDQVVGHARSVRLPgFVRHDTFYSLHE 177
Cdd:COG0463  161 eELGFDEGFLEDTELLRALRHGFR-IAEVPVRYRAGE 196
WcaE COG1216
Glycosyltransferase, GT2 family [Carbohydrate transport and metabolism];
1-94 6.34e-13

Glycosyltransferase, GT2 family [Carbohydrate transport and metabolism];


Pssm-ID: 440829 [Multi-domain]  Cd Length: 202  Bit Score: 65.78  E-value: 6.34e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344   1 MLKLSVCLLTCNSARLLMEVLPPLLK---VADECIIVDSGSTDETVSICQQF---GLTVHHHAYKA-HGAQMNYAIGLAS 73
Cdd:COG1216    2 RPKVSVVIPTYNRPELLRRCLESLLAqtyPPFEVIVVDNGSTDGTAELLAALafpRVRVIRNPENLgFAAARNLGLRAAG 81

                         90       100
                 ....*....|....*....|.
gi 496082344  74 HDWVLCMDSDEILDNDVVAAI 94
Cdd:COG1216   82 GDYLLFLDDDTVVEPDWLERL 102
Glycos_transf_2 pfam00535
Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, ...
 5-142 3.35e-12

Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.


Pssm-ID: 425738 [Multi-domain]  Cd Length: 166  Bit Score: 62.80  E-value: 3.35e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344    5 SVCLLTCNSARLLMEVLPPLLKV---ADECIIVDSGSTDETVSICQQF-----GLTVHHHAYK-AHGAQMNYAIGLASHD 75
Cdd:pfam00535   1 SVIIPTYNEEKYLLETLESLLNQtypNFEIIVVDDGSTDGTVEIAEEYakkdpRVRVIRLPENrGKAGARNAGLRAATGD 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 496082344   76 WVLCMDSDEILDNDVVAAIQALKAGEEPDPTCAWRLPRYWFVLGTQVRTIYPISSPDYPVRLFNRQQ 142
Cdd:pfam00535  81 YIAFLDADDEVPPDWLEKLVEALEEDGADVVVGSRYVIFGETGEYRRASRITLSRLPFFLGLRLLGL 147
Glyco_tranf_GTA_type cd00761
Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a ...
 6-116 8.62e-11

Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold; Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.


Pssm-ID: 132997 [Multi-domain]  Cd Length: 156  Bit Score: 58.67  E-value: 8.62e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344   6 VCLLTCNSARLLMEVLPPLLKV---ADECIIVDSGSTDETVSICQQFGLT------VHHHAYKAHGAQMNYAIGLASHDW 76
Cdd:cd00761    1 VIIPAYNEEPYLERCLESLLAQtypNFEVIVVDDGSTDGTLEILEEYAKKdprvirVINEENQGLAAARNAGLKAARGEY 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 496082344  77 VLCMDSDEILDNDVVAAiQALKAGEEPDPTCAWRLPRYWF 116
Cdd:cd00761   81 ILFLDADDLLLPDWLER-LVAELLADPEADAVGGPGNLLF 119
BcsA COG1215
Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1, ...
 1-100 7.06e-10

Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility];


Pssm-ID: 440828 [Multi-domain]  Cd Length: 303  Bit Score: 58.21  E-value: 7.06e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344   1 MLKLSVCLLTCNSARLLMEVLPPLL-----KVADECIIVDSGSTDETVSICQQFG-----LTVHHHAYKAH-GAQMNYAI 69
Cdd:COG1215   28 LPRVSVIIPAYNEEAVIEETLRSLLaqdypKEKLEVIVVDDGSTDETAEIARELAaeyprVRVIERPENGGkAAALNAGL 107

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 496082344  70 GLASHDWVLCMDSDEILDND----VVAAIQALKAG 100
Cdd:COG1215  108 KAARGDIVVFLDADTVLDPDwlrrLVAAFADPGVG 142
GT_2_WfgS_like cd06433
WfgS and WfeV are involved in O-antigen biosynthesis; Escherichia coli WfgS and Shigella ...
 30-145 2.17e-08

WfgS and WfeV are involved in O-antigen biosynthesis; Escherichia coli WfgS and Shigella dysenteriae WfeV are glycosyltransferase 2 family enzymes involved in O-antigen biosynthesis. GT-2 enzymes have GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133055 [Multi-domain]  Cd Length: 202  Bit Score: 52.93  E-value: 2.17e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344  30 ECIIVDSGSTDETVSICQQFGLTVHH----------HAykahgaqMNYAIGLASHDWVLCMDSDEIL-DNDVVAAIQALK 98
Cdd:cd06433   29 EYIVIDGGSTDGTVDIIKKYEDKITYwisepdkgiyDA-------MNKGIALATGDIIGFLNSDDTLlPGALLAVVAAFA 101

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 496082344  99 AGEEPDPTCAwrlPRYWFVLGTQVRTIYPISSPDYPVRLFNR---QQARF 145
Cdd:cd06433  102 EHPEVDVVYG---DVLLVDENGRVIGRRRPPPFLDKFLLYGMpicHQATF 148
CESA_like cd06423
CESA_like is the cellulose synthase superfamily; The cellulose synthase (CESA) superfamily ...
 29-105 4.75e-08

CESA_like is the cellulose synthase superfamily; The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.


Pssm-ID: 133045 [Multi-domain]  Cd Length: 180  Bit Score: 51.46  E-value: 4.75e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344  29 DECIIVDSGSTDETVSICQQFG-------LTVHHHAYKAHGAQMNYAIGLASHDWVLCMDSDEILDNDvvaAIQALKAGE 101
Cdd:cd06423   27 LEVIVVDDGSTDDTLEILEELAalyirrvLVVRDKENGGKAGALNAGLRHAKGDIVVVLDADTILEPD---ALKRLVVPF 103


                 ....
gi 496082344 102 EPDP 105
Cdd:cd06423  104 FADP 107
DPM_DPG-synthase_like cd04179
DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the ...
 12-144 9.58e-08

DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.


Pssm-ID: 133022 [Multi-domain]  Cd Length: 185  Bit Score: 50.65  E-value: 9.58e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344  12 NSARLLMEVLPPLLKVA-----DECIIVDSGSTDETVSICQQFGLTVHHHAYKAH------GAQMNYAIGLASHDWVLCM 80
Cdd:cd04179    7 NEEENIPELVERLLAVLeegydYEIIVVDDGSTDGTAEIARELAARVPRVRVIRLsrnfgkGAAVRAGFKAARGDIVVTM 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344  81 DSD------EIL---------DNDVVAAIQALKAGEEPDPtcaW--RLPRYWFVLGTQVRTIYPISSPDYPVRLFNRQQA 143
Cdd:cd04179   87 DADlqhppeDIPkllekllegGADVVIGSRFVRGGGAGMP---LlrRLGSRLFNFLIRLLLGVRISDTQSGFRLFRREVL 163


                 .
gi 496082344 144 R 144
Cdd:cd04179  164 E 164
GT_2_like_a cd02522
GT_2_like_a represents a glycosyltransferase family-2 subfamily with unknown function; ...
 14-94 1.28e-07

GT_2_like_a represents a glycosyltransferase family-2 subfamily with unknown function; Glycosyltransferase family 2 (GT-2) subfamily of unknown function. GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133013 [Multi-domain]  Cd Length: 221  Bit Score: 51.03  E-value: 1.28e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344  14 ARLLmEVLPPLLKVADECIIVDSGSTDETVSICQQFGLTVHHHAyKAHGAQMNYAIGLASHDWVLCMDSDEILDNDVVAA 93
Cdd:cd02522   15 PRLL-ASLRRLNPLPLEIIVVDGGSTDGTVAIARSAGVVVISSP-KGRARQMNAGAAAARGDWLLFLHADTRLPPDWDAA 92


                 .
gi 496082344  94 I 94
Cdd:cd02522   93 I 93
Succinoglycan_BP_ExoA cd02525
ExoA is involved in the biosynthesis of succinoglycan; Succinoglycan Biosynthesis Protein ExoA ...
 26-99 5.30e-07

ExoA is involved in the biosynthesis of succinoglycan; Succinoglycan Biosynthesis Protein ExoA catalyzes the formation of a beta-1,3 linkage of the second sugar (glucose) of the succinoglycan with the galactose on the lipid carrie. Succinoglycan is an acidic exopolysaccharide that is important for invasion of the nodules. Succinoglycan is a high-molecular-weight polymer composed of repeating octasaccharide units. These units are synthesized on membrane-bound isoprenoid lipid carriers, beginning with galactose followed by seven glucose molecules, and modified by the addition of acetate, succinate, and pyruvate. ExoA is a membrane protein with a transmembrance domain at c-terminus.


Pssm-ID: 133016 [Multi-domain]  Cd Length: 249  Bit Score: 49.54  E-value: 5.30e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344  26 KVADECIIVDSGSTDETVSICQQFG-----LTVHHHAYKAHGAQMNYAIGLASHDWVLCMDSDEILDND-VVAAIQALKA 99
Cdd:cd02525   29 KDLIEIIVVDGGSTDGTREIVQEYAakdprIRLIDNPKRIQSAGLNIGIRNSRGDIIIRVDAHAVYPKDyILELVEALKR 108
GT_2_like_d cd04196
Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse ...
 5-104 2.00e-06

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133039 [Multi-domain]  Cd Length: 214  Bit Score: 47.24  E-value: 2.00e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344   5 SVCLLTCNSARLLMEVLPPLLK---VADECIIVDSGSTDETVSICQQFGLTVHHHAY-----KAHGAQMNY--AIGLASH 74
Cdd:cd04196    1 AVLMATYNGEKYLREQLDSILAqtyKNDELIISDDGSTDGTVEIIKEYIDKDPFIIIlirngKNLGVARNFesLLQAADG 80

                         90       100       110
                 ....*....|....*....|....*....|.
gi 496082344  75 DWVLCMDSDEI-LDNDVVAAIQALKAGEEPD 104
Cdd:cd04196   81 DYVFFCDQDDIwLPDKLERLLKAFLKDDKPL 111
CESA_like_1 cd06439
CESA_like_1 is a member of the cellulose synthase (CESA) superfamily; This is a subfamily of ...
 30-99 7.06e-05

CESA_like_1 is a member of the cellulose synthase (CESA) superfamily; This is a subfamily of cellulose synthase (CESA) superfamily. CESA superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members of the superfamily include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins.


Pssm-ID: 133061 [Multi-domain]  Cd Length: 251  Bit Score: 42.95  E-value: 7.06e-05
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 496082344  30 ECIIVDSGSTDETVSICQQF---GLTVHH---HAYKAHGaqMNYAIGLASHDWVLCMDSDEILDNDvvaAIQALKA 99
Cdd:cd06439   62 EIIVVSDGSTDGTAEIAREYadkGVKLLRfpeRRGKAAA--LNRALALATGEIVVFTDANALLDPD---ALRLLVR 132
Glyco_tranf_2_4 pfam13704
Glycosyl transferase family 2; Members of this family of prokaryotic proteins include putative ...
 28-89 8.69e-05

Glycosyl transferase family 2; Members of this family of prokaryotic proteins include putative glucosyltransferases,


Pssm-ID: 433416 [Multi-domain]  Cd Length: 97  Bit Score: 40.69  E-value: 8.69e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 496082344   28 ADECIIVDSGSTDETVSICQQ---FGLTVHHHAYKAHGAQMNYAIGLASH----DWVLCMDSDEILDND 89
Cdd:pfam13704  19 FDHIYVYDNGSDDGTAEILARlpdVSILRSDLSYKDARFQVDWRNALLARyaeaDWVLVVDADEFLVYP 87
GT_2_like_b cd04185
Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse ...
 9-107 1.15e-04

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133028 [Multi-domain]  Cd Length: 202  Bit Score: 41.85  E-value: 1.15e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344   9 LTCNSARLLMEVLPPLLKV---ADECIIVDSGSTDETVSICQQFG----LTVHHHAYKAHGA-------QMNYAIGlasH 74
Cdd:cd04185    4 VTYNRLDLLKECLDALLAQtrpPDHIIVIDNASTDGTAEWLTSLGdldnIVYLRLPENLGGAggfyegvRRAYELG---Y 80

                         90       100       110
                 ....*....|....*....|....*....|...
gi 496082344  75 DWVLCMDSDEILDNDVVAAIqaLKAGEEPDPTC 107
Cdd:cd04185   81 DWIWLMDDDAIPDPDALEKL--LAYADKDNPQF 111
PRK13915 PRK13915
putative glucosyl-3-phosphoglycerate synthase; Provisional
 1-55 4.49e-04

putative glucosyl-3-phosphoglycerate synthase; Provisional


Pssm-ID: 237556 [Multi-domain]  Cd Length: 306  Bit Score: 40.67  E-value: 4.49e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 496082344   1 MLKLSVCLLTCNSA-----------RLLMEVLPpllkvaDECIIVDSGSTDETVSICQQFGLTVHH 55
Cdd:PRK13915  30 GRTVSVVLPALNEEetvgkvvdsirPLLMEPLV------DELIVIDSGSTDATAERAAAAGARVVS 89
GT_2_like_c cd04186
Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse ...
 12-104 7.04e-04

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133029 [Multi-domain]  Cd Length: 166  Bit Score: 39.08  E-value: 7.04e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344  12 NSARLLMEVLPPLLK---VADECIIVDSGSTDETVSICQQFGLTVHHHAYKAH---GAQMNYAIGLASHDWVLCMDSDEI 85
Cdd:cd04186    7 NSLEYLKACLDSLLAqtyPDFEVIVVDNASTDGSVELLRELFPEVRLIRNGENlgfGAGNNQGIREAKGDYVLLLNPDTV 86

                         90
                 ....*....|....*....
gi 496082344  86 LDNDVVAAiqALKAGEEPD 104
Cdd:cd04186   87 VEPGALLE--LLDAAEQDP 103
GT_2_like_e cd04192
Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse ...
 30-83 2.06e-03

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133035 [Multi-domain]  Cd Length: 229  Bit Score: 38.42  E-value: 2.06e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 496082344  30 ECIIVDSGSTDETVSICQQFGLTVHHH-----------AYKAHGAQMnyAIGLASHDWVLCMDSD 83
Cdd:cd04192   30 EVILVDDHSTDGTVQILEFAAAKPNFQlkilnnsrvsiSGKKNALTT--AIKAAKGDWIVTTDAD 92
DPM1_like_bac cd04187
Bacterial DPM1_like enzymes are related to eukaryotic DPM1; A family of bacterial enzymes ...
 19-96 7.03e-03

Bacterial DPM1_like enzymes are related to eukaryotic DPM1; A family of bacterial enzymes related to eukaryotic DPM1; Although the mechanism of eukaryotic enzyme is well studied, the mechanism of the bacterial enzymes is not well understood. The eukaryotic DPM1 is the catalytic subunit of eukaryotic Dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. The enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. This protein family belongs to Glycosyltransferase 2 superfamily.


Pssm-ID: 133030 [Multi-domain]  Cd Length: 181  Bit Score: 36.30  E-value: 7.03e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496082344  19 EVLPPLL----KVAD------ECIIVDSGSTDETVSICQQ----------------FGltvhHHAykAHGAQMNYAIGla 72
Cdd:cd04187   10 ENLPELYerlkAVLEslgydyEIIFVDDGSTDRTLEILRElaardprvkvirlsrnFG----QQA--ALLAGLDHARG-- 81

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 496082344  73 shDWVLCMDSD------EIL--------DNDVVAAIQA 96
Cdd:cd04187   82 --DAVITMDADlqdppeLIPemlakweeGYDVVYGVRK 117
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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