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Conserved domains on  [gi|496341765|ref|WP_009050943|]
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MULTISPECIES: GspH/FimT family protein [Pseudomonas]

Protein Classification

GspH/FimT family protein( domain architecture ID 11450583)

GspH/FimT family protein similar to Escherichia coli General secretion pathway protein H (GspH) which is a minor pseudopilin and may function as an initiator or regulator of pilus biogenesis and dynamics, and/or as an adaptor between various pseudopilin component and other members of the type II secretion system (T2SS)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GspH pfam12019
Type II transport protein GspH; GspH is involved in bacterial type II export systems. Like all ...
 41-156 1.45e-15

Type II transport protein GspH; GspH is involved in bacterial type II export systems. Like all pilins, GspH has an N terminus alpha helix. This helix is followed by nine beta strands forming two beta sheets, one of five antiparallel strands and one of four antiparallel strands. GspH is a minor pseudopilin; it is expressed much less than other pseudopilins in the type II secretion pilus (major pilins). The function and localization of minor pseudo-pilins are still to be fully unraveled. It has been suggested that some minor pseudopilins may assemble either into the base or the tip of pili, or both. They function as initiators or regulators of pilus biogenesis and dynamics, and/or as adaptors between various pseudopilin component and other members of the T2SS.


:

Pssm-ID: 463433  Cd Length: 108  Bit Score: 68.77  E-value: 1.45e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496341765   41 AAQMLASGIRNARTEAILRQQRIVIHGlnDDWSQGWRIILDISGEGSQDGDNPVLLERRGDARVAI-VGNRPVQHFVRFS 119
Cdd:pfam12019   1 AANRLAAALQLARSEAIKRGRPVTVCP--SDWSGGWIVFVDANANGDLDGGEDLLRVGAALAGGDVlVTASSASPQITFN 78

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 496341765  120 GLGDpllpsgAFQAGTLHICGADPGTSqHQVVLSRSG 156
Cdd:pfam12019  79 PDGR------AATPGTLTLCSGGSGRS-RRVVVSASG 108
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
2-65 1.39e-14

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


:

Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 64.87  E-value: 1.39e-14
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 496341765   2 PQKGFGLIEIIVGLALASLLMLFATTSFGELFQSTRRLDAAQMLASGIRNARTEAILRQQRIVI 65
Cdd:COG4970    7 RQRGFTLIELLVVLAILAILAAIAVPSFSSLIARQRLRAAANELAAALRLARSEAIRRGQPVTL 70
 
Name Accession Description Interval E-value
GspH pfam12019
Type II transport protein GspH; GspH is involved in bacterial type II export systems. Like all ...
 41-156 1.45e-15

Type II transport protein GspH; GspH is involved in bacterial type II export systems. Like all pilins, GspH has an N terminus alpha helix. This helix is followed by nine beta strands forming two beta sheets, one of five antiparallel strands and one of four antiparallel strands. GspH is a minor pseudopilin; it is expressed much less than other pseudopilins in the type II secretion pilus (major pilins). The function and localization of minor pseudo-pilins are still to be fully unraveled. It has been suggested that some minor pseudopilins may assemble either into the base or the tip of pili, or both. They function as initiators or regulators of pilus biogenesis and dynamics, and/or as adaptors between various pseudopilin component and other members of the T2SS.


Pssm-ID: 463433  Cd Length: 108  Bit Score: 68.77  E-value: 1.45e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496341765   41 AAQMLASGIRNARTEAILRQQRIVIHGlnDDWSQGWRIILDISGEGSQDGDNPVLLERRGDARVAI-VGNRPVQHFVRFS 119
Cdd:pfam12019   1 AANRLAAALQLARSEAIKRGRPVTVCP--SDWSGGWIVFVDANANGDLDGGEDLLRVGAALAGGDVlVTASSASPQITFN 78

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 496341765  120 GLGDpllpsgAFQAGTLHICGADPGTSqHQVVLSRSG 156
Cdd:pfam12019  79 PDGR------AATPGTLTLCSGGSGRS-RRVVVSASG 108
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
2-65 1.39e-14

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 64.87  E-value: 1.39e-14
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 496341765   2 PQKGFGLIEIIVGLALASLLMLFATTSFGELFQSTRRLDAAQMLASGIRNARTEAILRQQRIVI 65
Cdd:COG4970    7 RQRGFTLIELLVVLAILAILAAIAVPSFSSLIARQRLRAAANELAAALRLARSEAIRRGQPVTL 70
TIGR02596 TIGR02596
Verru_Chthon cassette protein D; This model describes a nearly twenty member protein family in ...
 6-90 7.65e-04

Verru_Chthon cassette protein D; This model describes a nearly twenty member protein family in Verrucomicrobium spinosum and a somewhat smaller paralogous family in Chthoniobacter flavus. All members share a type IV pilin-like N-terminal leader sequence (TIGR02532). These proteins occur in the four-gene Verru_Chthon cassette, in which two other genes likewise encode a cleavage/methylation domain. Most of these cassettes occur next to an unusually large PEP-CTERM protein with an autotransporter domain. [Cell envelope, Surface structures]


Pssm-ID: 274219 [Multi-domain]  Cd Length: 195  Bit Score: 38.61  E-value: 7.65e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496341765    6 FGLIEIIVGLALASLLMLFATTSFGELFQSTRRLDAAQMLASGIRNARTEAILRQ--------QRIVIHGLNDDWSQGWR 77
Cdd:TIGR02596   1 FTLVELLVVIAIAALLMALSTPVVNQVLAAQQLGSSATRLANELAAAAQLAVRENrpvevrflRRADDAGPGALGYRGWQ 80

                          90
                  ....*....|...
gi 496341765   78 IILDISGEGSQDG 90
Cdd:TIGR02596  81 LMVPEPTTIGKWR 93
ComGD NF040982
competence type IV pilus minor pilin ComGD;
3-66 1.36e-03

competence type IV pilus minor pilin ComGD;


Pssm-ID: 468912 [Multi-domain]  Cd Length: 140  Bit Score: 37.22  E-value: 1.36e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 496341765   3 QKGFGLIE-IIVGLALASLLMLFATTSFGELFQS-TRRLDAAQmLASGIRNARTEAILRQQRIVIH 66
Cdd:NF040982   1 IKGFTLLEsLLVLLIVSSLLLLTLSLSIPQTYEQvEEKLFFEQ-FENDLLYAQQYAIASQQSTTIT 65
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 3-26 2.48e-03

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 33.89  E-value: 2.48e-03
                          10        20
                  ....*....|....*....|....
gi 496341765    3 QKGFGLIEIIVGLALASLLMLFAT 26
Cdd:pfam07963   4 QRGFTLIELLVALAILAILLAAAL 27
typeII_sec_gspH TIGR01708
type II secretion system protein H; This model represents GspH, protein H of the main terminal ...
 3-77 2.96e-03

type II secretion system protein H; This model represents GspH, protein H of the main terminal branch of the general secretion pathway, also called type II secretion. It transports folded proteins across the bacterial outer membrane and is widely distributed in Gram-negative pathogens. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


Pssm-ID: 130769 [Multi-domain]  Cd Length: 143  Bit Score: 36.39  E-value: 2.96e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 496341765    3 QKGFGLIEIIVGLALASLLMLFATTSFGELFQSTRRLDAAQMLASGIRNARTEAILRQQRIVIhglndDW-SQGWR 77
Cdd:TIGR01708   3 QSGFTLIELLVVLAIMGLVAAAAALSLVSHYGTKSLDQVAGRLAARLRLAQTSARATGRPVLL-----RLgSRGIR 73
 
Name Accession Description Interval E-value
GspH pfam12019
Type II transport protein GspH; GspH is involved in bacterial type II export systems. Like all ...
 41-156 1.45e-15

Type II transport protein GspH; GspH is involved in bacterial type II export systems. Like all pilins, GspH has an N terminus alpha helix. This helix is followed by nine beta strands forming two beta sheets, one of five antiparallel strands and one of four antiparallel strands. GspH is a minor pseudopilin; it is expressed much less than other pseudopilins in the type II secretion pilus (major pilins). The function and localization of minor pseudo-pilins are still to be fully unraveled. It has been suggested that some minor pseudopilins may assemble either into the base or the tip of pili, or both. They function as initiators or regulators of pilus biogenesis and dynamics, and/or as adaptors between various pseudopilin component and other members of the T2SS.


Pssm-ID: 463433  Cd Length: 108  Bit Score: 68.77  E-value: 1.45e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496341765   41 AAQMLASGIRNARTEAILRQQRIVIHGlnDDWSQGWRIILDISGEGSQDGDNPVLLERRGDARVAI-VGNRPVQHFVRFS 119
Cdd:pfam12019   1 AANRLAAALQLARSEAIKRGRPVTVCP--SDWSGGWIVFVDANANGDLDGGEDLLRVGAALAGGDVlVTASSASPQITFN 78

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 496341765  120 GLGDpllpsgAFQAGTLHICGADPGTSqHQVVLSRSG 156
Cdd:pfam12019  79 PDGR------AATPGTLTLCSGGSGRS-RRVVVSASG 108
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
2-65 1.39e-14

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 64.87  E-value: 1.39e-14
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 496341765   2 PQKGFGLIEIIVGLALASLLMLFATTSFGELFQSTRRLDAAQMLASGIRNARTEAILRQQRIVI 65
Cdd:COG4970    7 RQRGFTLIELLVVLAILAILAAIAVPSFSSLIARQRLRAAANELAAALRLARSEAIRRGQPVTL 70
PilW COG4966
Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];
3-52 1.95e-04

Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];


Pssm-ID: 443992 [Multi-domain]  Cd Length: 158  Bit Score: 39.78  E-value: 1.95e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 496341765   3 QKGFGLIEIIVGLALASLLMLFATTSFGELFQSTRRLDAAQMLASGIRNA 52
Cdd:COG4966    4 QRGFTLVELMVALAIGLIVLAAVLQLFLSSRRSYRTQEALARLQENGRFA 53
PulG COG2165
Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, ...
 3-52 3.60e-04

Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 441768 [Multi-domain]  Cd Length: 99  Bit Score: 37.97  E-value: 3.60e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 496341765   3 QKGFGLIEIIVGLALASLLMLFATTSFGELFQSTRRLDAAQMLASgIRNA 52
Cdd:COG2165   10 QRGFTLIELLVVIAIIGILAALALPALQGARERARRAELRSNLRQ-IQQA 58
PulJ COG4795
Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and ...
 3-52 6.51e-04

Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443823 [Multi-domain]  Cd Length: 118  Bit Score: 37.69  E-value: 6.51e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 496341765   3 QKGFGLIEIIVGLALASLLMLFATTSFGELFQSTRRLDAAQMLASGIRNA 52
Cdd:COG4795    8 QRGFTLLELLVALAIFALLLLAAYRGLDSVLRSRERLEQQAERLQELQRA 57
TIGR02596 TIGR02596
Verru_Chthon cassette protein D; This model describes a nearly twenty member protein family in ...
 6-90 7.65e-04

Verru_Chthon cassette protein D; This model describes a nearly twenty member protein family in Verrucomicrobium spinosum and a somewhat smaller paralogous family in Chthoniobacter flavus. All members share a type IV pilin-like N-terminal leader sequence (TIGR02532). These proteins occur in the four-gene Verru_Chthon cassette, in which two other genes likewise encode a cleavage/methylation domain. Most of these cassettes occur next to an unusually large PEP-CTERM protein with an autotransporter domain. [Cell envelope, Surface structures]


Pssm-ID: 274219 [Multi-domain]  Cd Length: 195  Bit Score: 38.61  E-value: 7.65e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 496341765    6 FGLIEIIVGLALASLLMLFATTSFGELFQSTRRLDAAQMLASGIRNARTEAILRQ--------QRIVIHGLNDDWSQGWR 77
Cdd:TIGR02596   1 FTLVELLVVIAIAALLMALSTPVVNQVLAAQQLGSSATRLANELAAAAQLAVRENrpvevrflRRADDAGPGALGYRGWQ 80

                          90
                  ....*....|...
gi 496341765   78 IILDISGEGSQDG 90
Cdd:TIGR02596  81 LMVPEPTTIGKWR 93
ComGD NF040982
competence type IV pilus minor pilin ComGD;
3-66 1.36e-03

competence type IV pilus minor pilin ComGD;


Pssm-ID: 468912 [Multi-domain]  Cd Length: 140  Bit Score: 37.22  E-value: 1.36e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 496341765   3 QKGFGLIE-IIVGLALASLLMLFATTSFGELFQS-TRRLDAAQmLASGIRNARTEAILRQQRIVIH 66
Cdd:NF040982   1 IKGFTLLEsLLVLLIVSSLLLLTLSLSIPQTYEQvEEKLFFEQ-FENDLLYAQQYAIASQQSTTIT 65
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 3-26 2.48e-03

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 33.89  E-value: 2.48e-03
                          10        20
                  ....*....|....*....|....
gi 496341765    3 QKGFGLIEIIVGLALASLLMLFAT 26
Cdd:pfam07963   4 QRGFTLIELLVALAILAILLAAAL 27
typeII_sec_gspH TIGR01708
type II secretion system protein H; This model represents GspH, protein H of the main terminal ...
 3-77 2.96e-03

type II secretion system protein H; This model represents GspH, protein H of the main terminal branch of the general secretion pathway, also called type II secretion. It transports folded proteins across the bacterial outer membrane and is widely distributed in Gram-negative pathogens. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


Pssm-ID: 130769 [Multi-domain]  Cd Length: 143  Bit Score: 36.39  E-value: 2.96e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 496341765    3 QKGFGLIEIIVGLALASLLMLFATTSFGELFQSTRRLDAAQMLASGIRNARTEAILRQQRIVIhglndDW-SQGWR 77
Cdd:TIGR01708   3 QSGFTLIELLVVLAIMGLVAAAAALSLVSHYGTKSLDQVAGRLAARLRLAQTSARATGRPVLL-----RLgSRGIR 73
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 3-26 9.04e-03

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 32.28  E-value: 9.04e-03
                          10        20
                  ....*....|....*....|....
gi 496341765    3 QKGFGLIEIIVGLALASLLMLFAT 26
Cdd:TIGR02532   1 QRGFTLIELLVVLAILGILALIAL 24
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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