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Conserved domains on  [gi|497653360|ref|WP_009967544|]
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MULTISPECIES: bacteriocin sublancin-168 [Bacillus]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Sublancin super family cl41136
Sublancin; This family represents sublancin, a small bacteriocin active against Gram-positive ...
 1-56 5.80e-17

Sublancin; This family represents sublancin, a small bacteriocin active against Gram-positive bacteria. This family appears to be restricted to Bacilli. Sublancin was thought to be a lantibiotic but was later shown to be an S-linked glycopeptide. Glycosylation is essential for its antimicrobial activity. Sublancin is biosynthesized as a precursor peptide bearing an N-terminal leader peptide, and a C-terminal core peptide that is converted into the mature peptide. Sublancin comprises two alpha helices and a well-defined inter-helical loop. Sublancin inhibits B.cereus spore outgrowth, after the germination stage, approximately 1000-fold better than it inhibits exponential growth of the same cells and inhibits B.subtilis strain ATCC6633 and B. megaterium strain 14581.


The actual alignment was detected with superfamily member pfam19151:

Pssm-ID: 408921  Cd Length: 57  Bit Score: 66.50  E-value: 5.80e-17
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 497653360   1 MEKLFKEVKLEELENQKGSGLGKAQCAALWLQCASGGTIGCGGGAV-ACQNYRQFCR 56
Cdd:pfam19151  1 MDKLFKELKIDEIENFKGKGFGAAQCAAIALQCASGGAIGCGGGATqACDNYEQFCR 57
 
Name Accession Description Interval E-value
Sublancin pfam19151
Sublancin; This family represents sublancin, a small bacteriocin active against Gram-positive ...
 1-56 5.80e-17

Sublancin; This family represents sublancin, a small bacteriocin active against Gram-positive bacteria. This family appears to be restricted to Bacilli. Sublancin was thought to be a lantibiotic but was later shown to be an S-linked glycopeptide. Glycosylation is essential for its antimicrobial activity. Sublancin is biosynthesized as a precursor peptide bearing an N-terminal leader peptide, and a C-terminal core peptide that is converted into the mature peptide. Sublancin comprises two alpha helices and a well-defined inter-helical loop. Sublancin inhibits B.cereus spore outgrowth, after the germination stage, approximately 1000-fold better than it inhibits exponential growth of the same cells and inhibits B.subtilis strain ATCC6633 and B. megaterium strain 14581.


Pssm-ID: 408921  Cd Length: 57  Bit Score: 66.50  E-value: 5.80e-17
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 497653360   1 MEKLFKEVKLEELENQKGSGLGKAQCAALWLQCASGGTIGCGGGAV-ACQNYRQFCR 56
Cdd:pfam19151  1 MDKLFKELKIDEIENFKGKGFGAAQCAAIALQCASGGAIGCGGGATqACDNYEQFCR 57
glycopep_SunS TIGR04196
glycopeptide, sublancin family; Members of this family, including sublancin, are ...
 1-55 1.38e-13

glycopeptide, sublancin family; Members of this family, including sublancin, are post-ribosomal natural products (PRNP) with an S-linked glycosylation. Sublancin itself also has two disulfide bonds. A related gene cluster in Bacillus cereus E33L includes the four Cys involved in the disulfide cluster but lacks the region with the glycosylated Cys, and have been excluded.


Pssm-ID: 211919  Cd Length: 80  Bit Score: 58.70  E-value: 1.38e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 497653360   1 MEKLFKEVKLEELENQK-------------------GSGLGKAQCAALWLQCASGGTIGCGGG------AVACQNYRQFC 55
Cdd:TIGR04196  1 MEELFKELKLEELENFEggydgvnymhqhdgggaggGSGIGKAQCAAFKALCASGGSEWLGGYggcgggANACELARKFC 80
 
Name Accession Description Interval E-value
Sublancin pfam19151
Sublancin; This family represents sublancin, a small bacteriocin active against Gram-positive ...
 1-56 5.80e-17

Sublancin; This family represents sublancin, a small bacteriocin active against Gram-positive bacteria. This family appears to be restricted to Bacilli. Sublancin was thought to be a lantibiotic but was later shown to be an S-linked glycopeptide. Glycosylation is essential for its antimicrobial activity. Sublancin is biosynthesized as a precursor peptide bearing an N-terminal leader peptide, and a C-terminal core peptide that is converted into the mature peptide. Sublancin comprises two alpha helices and a well-defined inter-helical loop. Sublancin inhibits B.cereus spore outgrowth, after the germination stage, approximately 1000-fold better than it inhibits exponential growth of the same cells and inhibits B.subtilis strain ATCC6633 and B. megaterium strain 14581.


Pssm-ID: 408921  Cd Length: 57  Bit Score: 66.50  E-value: 5.80e-17
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 497653360   1 MEKLFKEVKLEELENQKGSGLGKAQCAALWLQCASGGTIGCGGGAV-ACQNYRQFCR 56
Cdd:pfam19151  1 MDKLFKELKIDEIENFKGKGFGAAQCAAIALQCASGGAIGCGGGATqACDNYEQFCR 57
glycopep_SunS TIGR04196
glycopeptide, sublancin family; Members of this family, including sublancin, are ...
 1-55 1.38e-13

glycopeptide, sublancin family; Members of this family, including sublancin, are post-ribosomal natural products (PRNP) with an S-linked glycosylation. Sublancin itself also has two disulfide bonds. A related gene cluster in Bacillus cereus E33L includes the four Cys involved in the disulfide cluster but lacks the region with the glycosylated Cys, and have been excluded.


Pssm-ID: 211919  Cd Length: 80  Bit Score: 58.70  E-value: 1.38e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 497653360   1 MEKLFKEVKLEELENQK-------------------GSGLGKAQCAALWLQCASGGTIGCGGG------AVACQNYRQFC 55
Cdd:TIGR04196  1 MEELFKELKLEELENFEggydgvnymhqhdgggaggGSGIGKAQCAAFKALCASGGSEWLGGYggcgggANACELARKFC 80
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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