|
Name |
Accession |
Description |
Interval |
E-value |
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
3-544 |
0e+00 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 1045.69 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 3 NSYEQDLDRNAANHQPLTPLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPM 82
Cdd:PRK08162 2 NIYEQGLDRNAANYVPLTPLSFLERAAEVYPDRPAVIHGDRRRTWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAM 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 83 LEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVT-PLVIDYDDPDYAAdapypkGE 161
Cdd:PRK08162 82 VEAHFGVPMAGAVLNTLNTRLDAASIAFMLRHGEAKVLIVDTEFAEVAREALALLPGPkPLVIDVDDPEYPG------GR 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 162 RIGALDYEDFVAGGDEDFAWSMPDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHC 241
Cdd:PRK08162 156 FIGALDYEAFLASGDPDFAWTLPADEWDAIALNYTSGTTGNPKGVVYHHRGAYLNALSNILAWGMPKHPVYLWTLPMFHC 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 242 NGWCFPWTLAVQAGTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSG 321
Cdd:PRK08162 236 NGWCFPWTVAARAGTNVCLRKVDPKLIFDLIREHGVTHYCGAPIVLSALINAPAEWRAGIDHPVHAMVAGAAPPAAVIAK 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 322 MADAGFAVTHLYGLTETYGPAVVNEWHGEWDDLAKGERSAKKARQGVRYAALEGLTVMDPETMQATPADGETIGEVMFRG 401
Cdd:PRK08162 316 MEEIGFDLTHVYGLTETYGPATVCAWQPEWDALPLDERAQLKARQGVRYPLQEGVTVLDPDTMQPVPADGETIGEIMFRG 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 402 NIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDD 481
Cdd:PRK08162 396 NIVMKGYLKNPKATEEAFAGGWFHTGDLAVLHPDGYIKIKDRSKDIIISGGENISSIEVEDVLYRHPAVLVAAVVAKPDP 475
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 499217198 482 KWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAEIPKTSTGKIQKFRLREMAK 544
Cdd:PRK08162 476 KWGEVPCAFVELKDGASATEEEIIAHCREHLAGFKVPKAVVFGELPKTSTGKIQKFVLREQAK 538
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
16-541 |
0e+00 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 823.09 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 16 HQPLTPLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAV 95
Cdd:cd12118 1 YVPLTPLSFLERAAAVYPDRTSIVYGDRRYTWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 96 LHSLNTRLDAAVIAFQLDHAETKVLIVDREFsgvvrqaldlakvtplvidyddpdyaadapypkgerigalDYEDFVAGG 175
Cdd:cd12118 81 LNALNTRLDAEEIAFILRHSEAKVLFVDREF----------------------------------------EYEDLLAEG 120
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 176 DEDFAWSMPDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAG 255
Cdd:cd12118 121 DPDFEWIPPADEWDPIALNYTSGTTGRPKGVVYHHRGAYLNALANILEWEMKQHPVYLWTLPMFHCNGWCFPWTVAAVGG 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 256 THVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADAGFAVTHLYGL 335
Cdd:cd12118 201 TNVCLRKVDAKAIYDLIEKHKVTHFCGAPTVLNMLANAPPSDARPLPHRVHVMTAGAPPPAAVLAKMEELGFDVTHVYGL 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 336 TETYGPAVVNEWHGEWDDLAKGERSAKKARQGVRYAALEGLTVMDPETMQATPADGETIGEVMFRGNIVMKGYLKNRKAS 415
Cdd:cd12118 281 TETYGPATVCAWKPEWDELPTEERARLKARQGVRYVGLEEVDVLDPETMKPVPRDGKTIGEIVFRGNIVMKGYLKNPEAT 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 416 DEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKP 495
Cdd:cd12118 361 AEAFRGGWFHSGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKE 440
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 499217198 496 GKTASEAEIIEHCRALLARFKVPKAVIFAEIPKTSTGKIQKFRLRE 541
Cdd:cd12118 441 GAKVTEEEIIAFCREHLAGFMVPKTVVFGELPKTSTGKIQKFVLRD 486
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
8-544 |
0e+00 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 685.04 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 8 DLDRNAANHQPLTPLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHF 87
Cdd:PLN02479 9 DLPKNAANYTALTPLWFLERAAVVHPTRKSVVHGSVRYTWAQTYQRCRRLASALAKRSIGPGSTVAVIAPNIPAMYEAHF 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 88 GVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQALDL-------AKVTPLVIDYDDPDYAADAPYPKG 160
Cdd:PLN02479 89 GVPMAGAVVNCVNIRLNAPTIAFLLEHSKSEVVMVDQEFFTLAEEALKIlaekkksSFKPPLLIVIGDPTCDPKSLQYAL 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 161 ERiGALDYEDFVAGGDEDFAWSMPDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFH 240
Cdd:PLN02479 169 GK-GAIEYEKFLETGDPEFAWKPPADEWQSIALGYTSGTTASPKGVVLHHRGAYLMALSNALIWGMNEGAVYLWTLPMFH 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 241 CNGWCFPWTLAVQAGTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDK-RTFAQTVTFNTAAAPPPEAVL 319
Cdd:PLN02479 248 CNGWCFTWTLAALCGTNICLRQVTAKAIYSAIANYGVTHFCAAPVVLNTIVNAPKSETiLPLPRVVHVMTAGAAPPPSVL 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 320 SGMADAGFAVTHLYGLTETYGPAVVNEWHGEWDDLAKGERSAKKARQGVRYAALEGLTVMDPETMQATPADGETIGEVMF 399
Cdd:PLN02479 328 FAMSEKGFRVTHTYGLSETYGPSTVCAWKPEWDSLPPEEQARLNARQGVRYIGLEGLDVVDTKTMKPVPADGKTMGEIVM 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 400 RGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARH 479
Cdd:PLN02479 408 RGNMVMKGYLKNPKANEEAFANGWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARP 487
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 480 DDKWGEVPVAYVELKPG-----KTASEAEIIEHCRALLARFKVPKAVIFAEIPKTSTGKIQKFRLREMAK 544
Cdd:PLN02479 488 DERWGESPCAFVTLKPGvdksdEAALAEDIMKFCRERLPAYWVPKSVVFGPLPKTATGKIQKHVLRAKAK 557
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
14-544 |
0e+00 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 521.50 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 14 ANHQPLTPLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTK 93
Cdd:PLN03102 9 ANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAG 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 94 AVLHSLNTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQALDL-----AKVTPLVIDYDDPDYAADAPYPKgerigaLDY 168
Cdd:PLN03102 89 AVLNPINTRLDATSIAAILRHAKPKILFVDRSFEPLAREVLHLlssedSNLNLPVIFIHEIDFPKRPSSEE------LDY 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 169 EDFVAGGDE--DFAWSM--PDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGW 244
Cdd:PLN03102 163 ECLIQRGEPtpSLVARMfrIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCNGW 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 245 CFPWTLAVQAGTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMAD 324
Cdd:PLN03102 243 TFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRSGPVHVLTGGSPPPAALVKKVQR 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 325 AGFAVTHLYGLTETYGPAVVNEWHGEWDDLAKGERSAKKARQGVRYAALEGLTVMDPETMQATPADGETIGEVMFRGNIV 404
Cdd:PLN03102 323 LGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQMELKARQGVSILGLADVDVKNKETQESVPRDGKTMGEIVIKGSSI 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 405 MKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWG 484
Cdd:PLN03102 403 MKGYLKNPKATSEAFKHGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTWG 482
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 499217198 485 EVPVAYVELKPGKT----------ASEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLREMAK 544
Cdd:PLN03102 483 ETPCAFVVLEKGETtkedrvdklvTRERDLIEYCRENLPHFMCPRKVVFlQELPKNGNGKILKPKLRDIAK 553
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
21-544 |
1.41e-155 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 454.64 E-value: 1.41e-155
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 21 PLTY---LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLH 97
Cdd:PRK06187 5 PLTIgriLRHGARKHPDKEAVYFDGRRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAVLH 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 98 SLNTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVIDYDDPDYaadapypKGERIGALDYEDFVAGGDE 177
Cdd:PRK06187 85 PINIRLKPEEIAYILNDAEDRVVLVDSEFVPLLAAILPQLPTVRTVIVEGDGPA-------APLAPEVGEYEELLAAASD 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 178 DFAWsmPD-DEWDAISLNYTSGTTGNPKGVVYHHRGAALMayTNTIHAGMAKHA--VYLWTLPMFHCNGWCFPwTLAVQA 254
Cdd:PRK06187 158 TFDF--PDiDENDAAAMLYTSGTTGHPKGVVLSHRNLFLH--SLAVCAWLKLSRddVYLVIVPMFHVHAWGLP-YLALMA 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 255 G-THVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVL-SGMADAGFAVTHL 332
Cdd:PRK06187 233 GaKQVIPRRFDPENLLDLIETERVTFFFAVPTIWQMLLKAPRAYFVDFSSLRLVIYGGAALPPALLrEFKEKFGIDLVQG 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 333 YGLTETYGPAVVNewHGEWDDLAKGE--RSAKKARQGVRYAalegltVMDPEtMQATPADGETIGEVMFRGNIVMKGYLK 410
Cdd:PRK06187 313 YGMTETSPVVSVL--PPEDQLPGQWTkrRSAGRPLPGVEAR------IVDDD-GDELPPDGGEVGEIIVRGPWLMQGYWN 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 411 NRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAY 490
Cdd:PRK06187 384 RPEATAETIDGGWLHTGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVAV 463
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 499217198 491 VELKPGKTASEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLREMAK 544
Cdd:PRK06187 464 VVLKPGATLDAKELRAFLRGRLAKFKLPKRIAFVdELPRTSVGKILKRVLREQYA 518
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
24-544 |
9.26e-154 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 447.33 E-value: 9.26e-154
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 24 YLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRL 103
Cdd:COG0318 4 LLRRAAARHPDRPALVFGGRRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLNPRL 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 104 DAAVIAFQLDHAETKVLIVdrefsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvaggdedfawsm 183
Cdd:COG0318 84 TAEELAYILEDSGARALVT------------------------------------------------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 184 pddewdaISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAG-THVCLRW 262
Cdd:COG0318 103 -------ALILYTSGTTGRPKGVMLTHRNLLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPLLAGaTLVLLPR 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 263 VRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADA-GFAVTHLYGLTETYGP 341
Cdd:COG0318 176 FDPERVLELIERERVTVLFGVPTMLARLLRHPEFARYDLSSLRLVVSGGAPLPPELLERFEERfGVRIVEGYGLTETSPV 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 342 AVVNewhgewddlAKGERSAKKARQGVRYAALEgLTVMDPETMQAtpADGEtIGEVMFRGNIVMKGYLKNRKASDEAFAG 421
Cdd:COG0318 256 VTVN---------PEDPGERRPGSVGRPLPGVE-VRIVDEDGREL--PPGE-VGEIVVRGPNVMKGYWNDPEATAEAFRD 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 422 GWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASE 501
Cdd:COG0318 323 GWLRTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVVAFVVLRPGAELDA 402
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 499217198 502 AEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLREMAK 544
Cdd:COG0318 403 EELRAFLRERLARYKVPRRVEFvDELPRTASGKIDRRALRERYA 446
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
25-541 |
2.72e-142 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 420.50 E-value: 2.72e-142
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHiAII-----HGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSL 99
Cdd:cd12119 2 LEHAARLHGDR-EIVsrtheGEVHRYTYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHTI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 100 NTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQALD-LAKVTPLVIDYDDPDYAADAPypkgerIGALDYEDFVAGGDED 178
Cdd:cd12119 81 NPRLFPEQIAYIINHAEDRVVFVDRDFLPLLEAIAPrLPTVEHVVVMTDDAAMPEPAG------VGVLAYEELLAAESPE 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 179 FAWsmPD-DEWDAISLNYTSGTTGNPKGVVYHHRGAAL--MAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTlAVQAG 255
Cdd:cd12119 155 YDW--PDfDENTAAAICYTSGTTGNPKGVVYSHRSLVLhaMAALLTDGLGLSESDVVLPVVPMFHVNAWGLPYA-AAMVG 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 256 THVCL--RWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADAGFAVTHLY 333
Cdd:cd12119 232 AKLVLpgPYLDPASLAELIEREGVTFAAGVPTVWQGLLDHLEANGRDLSSLRRVVIGGSAVPRSLIEAFEERGVRVIHAW 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 334 GLTETYGPAVVNEWHGEWDDLAKGERSAKKARQGVRYAALEgLTVMDPETmQATPADGETIGEVMFRGNIVMKGYLKNRK 413
Cdd:cd12119 312 GMTETSPLGTVARPPSEHSNLSEDEQLALRAKQGRPVPGVE-LRIVDDDG-RELPWDGKAVGELQVRGPWVTKSYYKNDE 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 414 ASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVEL 493
Cdd:cd12119 390 ESEALTEDGWLRTGDVATIDEDGYLTITDRSKDVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVL 469
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 499217198 494 KPGKTASEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLRE 541
Cdd:cd12119 470 KEGATVTAEELLEFLADKVAKWWLPDDVVFVdEIPKTSTGKIDKKALRE 518
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
18-541 |
2.27e-130 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 389.87 E-value: 2.27e-130
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 18 PLTPLTYLERAAKTYPDHIaiiHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLH 97
Cdd:cd05915 1 LERAAALFGRKEVVSRLHT---GEVHRTTYAEVYQRARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLH 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 98 SLNTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQALDL-AKVTplvidyddpdyaadapYPKGERIGALDYEDFVAGGD 176
Cdd:cd05915 78 TANPRLSPKEIAYILNHAEDKVLLFDPNLLPLVEAIRGElKTVQ----------------HFVVMDEKAPEGYLAYEEAL 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 177 EDFAWSMPDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHA--VYLWTLPMFHCNGWCFPWTLAVQA 254
Cdd:cd05915 142 GEEADPVRVPERAACGMAYTTGTTGLPKGVVYSHRALVLHSLAASLVDGTALSEkdVVLPVVPMFHVNAWCLPYAATLVG 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 255 GTHVCLRWVR-PKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADAGFAVTHLY 333
Cdd:cd05915 222 AKQVLPGPRLdPASLVELFDGEGVTFTAGVPTVWLALADYLESTGHRLKTLRRLVVGGSAAPRSLIARFERMGVEVRQGY 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 334 GLTETYGPAVVNEWHGEWDDLAKGERSAKKARQGVRYAAlEGLTVMDPETMqATPADGETIGEVMFRGNIVMKGYLKNRK 413
Cdd:cd05915 302 GLTETSPVVVQNFVKSHLESLSEEEKLTLKAKTGLPIPL-VRLRVADEEGR-PVPKDGKALGEVQLKGPWITGGYYGNEE 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 414 ASD-EAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVE 492
Cdd:cd05915 380 ATRsALTPDGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQERPLAVVV 459
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 499217198 493 LKPGKtASEAEIIEHCRALLARFK-VPKAVIFA-EIPKTSTGKIQKFRLRE 541
Cdd:cd05915 460 PRGEK-PTPEELNEHLLKAGFAKWqLPDAYVFAeEIPRTSAGKFLKRALRE 509
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
25-536 |
1.40e-123 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 369.63 E-value: 1.40e-123
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLD 104
Cdd:cd17631 1 LRRRARRHPDRTALVFGGRSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLT 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 105 AAVIAFQLDHAETKVLIvdrefsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvaggdEDFAWsmp 184
Cdd:cd17631 81 PPEVAYILADSGAKVLF-------------------------------------------------------DDLAL--- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 185 ddewdaisLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHC---NGWCFPwTLAVqAGTHVCLR 261
Cdd:cd17631 103 --------LMYTSGTTGRPKGAMLTHRNLLWNAVNALAALDLGPDDVLLVVAPLFHIgglGVFTLP-TLLR-GGTVVILR 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 262 WVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADAGFAVTHLYGLTETYGP 341
Cdd:cd17631 173 KFDPETVLDLIERHRVTSFFLVPTMIQALLQHPRFATTDLSSLRAVIYGGAPMPERLLRALQARGVKFVQGYGMTETSPG 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 342 AVVNEwhgEWDDLAKgERSAKKARQGVRYAalegltVMDPETmqaTPADGETIGEVMFRGNIVMKGYLKNRKASDEAFAG 421
Cdd:cd17631 253 VTFLS---PEDHRRK-LGSAGRPVFFVEVR------IVDPDG---REVPPGEVGEIVVRGPHVMAGYWNRPEATAAAFRD 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 422 GWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASE 501
Cdd:cd17631 320 GWFHTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEAVVAVVVPRPGAELDE 399
|
490 500 510
....*....|....*....|....*....|....*.
gi 499217198 502 AEIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQK 536
Cdd:cd17631 400 DELIAHCRERLARYKIPKSVEFVdALPRNATGKILK 435
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
25-540 |
2.29e-103 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 318.74 E-value: 2.29e-103
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLD 104
Cdd:cd05936 5 LEEAARRFPDKTALIFMGRKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLNPLYT 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 105 AAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVidyddpdyaadapypkgerigaldyedfvaggDEDFAwsmp 184
Cdd:cd05936 85 PRELEHILNDSGAKALIVAVSFTDLLAAGAPLGERVALT--------------------------------PEDVA---- 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 185 ddewdaiSLNYTSGTTGNPKGVVYHHRgaALMAYTNTIHAGMA----KHAVYLWTLPMFHCNGWCFPWTLAVQAG-THVC 259
Cdd:cd05936 129 -------VLQYTSGTTGVPKGAMLTHR--NLVANALQIKAWLEdlleGDDVVLAALPLFHVFGLTVALLLPLALGaTIVL 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 260 LRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADA-GFAVTHLYGLTET 338
Cdd:cd05936 200 IPRFRPIGVLKEIRKHRVTIFPGVPTMYIALLNAPEFKKRDFSSLRLCISGGAPLPVEVAERFEELtGVPIVEGYGLTET 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 339 YGPAVVNewhgewddlakgersakkARQGVRYAALEGL-------TVMDPETmqATPADGETiGEVMFRGNIVMKGYLKN 411
Cdd:cd05936 280 SPVVAVN------------------PLDGPRKPGSIGIplpgtevKIVDDDG--EELPPGEV-GELWVRGPQVMKGYWNR 338
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 412 RKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYV 491
Cdd:cd05936 339 PEETAEAFVDGWLRTGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAVVGVPDPYSGEAVKAFV 418
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 499217198 492 ELKPGKTASEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLR 540
Cdd:cd05936 419 VLKEGASLTEEEIIAFCREQLAGYKVPRQVEFrDELPKSAVGKILRRELR 468
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
189-535 |
8.94e-97 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 297.27 E-value: 8.94e-97
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 DAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLRWVRPKPI 268
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHIGGLFGLLGALLAGGTVVLLPKFDPEAA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 269 YDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADA-GFAVTHLYGLTETygPAVVNEW 347
Cdd:cd04433 81 LELIEREKVTILLGVPTLLARLLKAPESAGYDLSSLRALVSGGAPLPPELLERFEEApGIKLVNGYGLTET--GGTVATG 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 348 HGewDDLAKGERSAKKARQGVRYAalegltVMDPETmqaTPADGETIGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSG 427
Cdd:cd04433 159 PP--DDDARKPGSVGRPVPGVEVR------IVDPDG---GELPPGEIGELVVRGPSVMKGYWNNPEATAAVDEDGWYRTG 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 428 DLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEH 507
Cdd:cd04433 228 DLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGADLDAEELRAH 307
|
330 340
....*....|....*....|....*....
gi 499217198 508 CRALLARFKVPKAVIF-AEIPKTSTGKIQ 535
Cdd:cd04433 308 VRERLAPYKVPRRVVFvDALPRTASGKID 336
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
25-541 |
9.61e-97 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 303.78 E-value: 9.61e-97
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLD 104
Cdd:PRK08316 17 LRRSARRYPDKTALVFGDRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLT 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 105 AAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVIDYDDPDYAADApypkgeriGALDYEDFVAGGDEDFAWSMP 184
Cdd:PRK08316 97 GEELAYILDHSGARAFLVDPALAPTAEAALALLPVDTLILSLVLGGREAPG--------GWLDFADWAEAGSVAEPDVEL 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 185 DDEwDAISLNYTSGTTGNPKGVVYHHRgaALMA-YTNTIHAG-MAKHAVYLWTLPMFHCNGW-CF--PWtLAVQAgTHVC 259
Cdd:PRK08316 169 ADD-DLAQILYTSGTESLPKGAMLTHR--ALIAeYVSCIVAGdMSADDIPLHALPLYHCAQLdVFlgPY-LYVGA-TNVI 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 260 LRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADA--GFAVTHLYGLTE 337
Cdd:PRK08316 244 LDAPDPELILRTIEAERITSFFAPPTVWISLLRHPDFDTRDLSSLRKGYYGASIMPVEVLKELRERlpGLRFYNCYGQTE 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 338 TyGPavvnewhgewddLAkgerSAKKARQGVRYAALEGLTVMDPET------MQATPaDGEtIGEVMFRGNIVMKGYLKN 411
Cdd:PRK08316 324 I-AP------------LA----TVLGPEEHLRRPGSAGRPVLNVETrvvdddGNDVA-PGE-VGEIVHRSPQLMLGYWDD 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 412 RKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYV 491
Cdd:PRK08316 385 PEKTAEAFRGGWFHSGDLGVMDEEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVV 464
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 499217198 492 ELKPGKTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKIQKFRLRE 541
Cdd:PRK08316 465 VPKAGATVTEDELIAHCRARLAGFKVPKRVIFVDeLPRNPSGKILKRELRE 515
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
25-451 |
1.02e-93 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 292.29 E-value: 1.02e-93
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGR-QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRL 103
Cdd:pfam00501 1 LERQAARTPDKTALEVGEgRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 104 DAAVIAFQLDHAETKVLIVDREFsgvvrQALDLAKVTPLVIDYDDPDYAADAPYPKGERIGALDYEDFVAGGDEDfawsm 183
Cdd:pfam00501 81 PAEELAYILEDSGAKVLITDDAL-----KLEELLEALGKLEVVKLVLVLDRDPVLKEEPLPEEAKPADVPPPPPP----- 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 184 PDDEWDAISLNYTSGTTGNPKGVVYHHRG----AALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVC 259
Cdd:pfam00501 151 PPDPDDLAYIIYTSGTTGKPKGVMLTHRNlvanVLSIKRVRPRGFGLGPDDRVLSTLPLFHDFGLSLGLLGPLLAGATVV 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 260 L--RWVRPKP--IYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADA-GFAVTHLYG 334
Cdd:pfam00501 231 LppGFPALDPaaLLELIERYKVTVLYGVPTLLNMLLEAGAPKRALLSSLRLVLSGGAPLPPELARRFRELfGGALVNGYG 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 335 LTETyGPAVVNEWHGEWDDLAKGerSAKKARQGVRYAalegltVMDPETMQATPaDGETiGEVMFRGNIVMKGYLKNRKA 414
Cdd:pfam00501 311 LTET-TGVVTTPLPLDEDLRSLG--SVGRPLPGTEVK------IVDDETGEPVP-PGEP-GELCVRGPGVMKGYLNDPEL 379
|
410 420 430
....*....|....*....|....*....|....*...
gi 499217198 415 SDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISG 451
Cdd:pfam00501 380 TAEAFdEDGWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
34-544 |
1.85e-89 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 285.11 E-value: 1.85e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 34 DHIAIIHGRQ------------RISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNT 101
Cdd:PRK06018 17 DHAARIHGNRevvtrsvegpivRTTYAQIHDRALKVSQALDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVNP 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 102 RLDAAVIAFQLDHAETKVLIVDREFSGVVRQALD-LAKVTPLVIDYDDPDYAADAPYpkgeriGALDYEDFVAGGDEDFA 180
Cdd:PRK06018 97 RLFPEQIAWIINHAEDRVVITDLTFVPILEKIADkLPSVERYVVLTDAAHMPQTTLK------NAVAYEEWIAEADGDFA 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 181 WSMPDdEWDAISLNYTSGTTGNPKGVVYHHRgaalmayTNTIHAGMAKHA---------VYLWTLPMFHCNGWCFPWTlA 251
Cdd:PRK06018 171 WKTFD-ENTAAGMCYTSGTTGDPKGVLYSHR-------SNVLHALMANNGdalgtsaadTMLPVVPLFHANSWGIAFS-A 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 252 VQAGThvclRWVRPKP------IYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADA 325
Cdd:PRK06018 242 PSMGT----KLVMPGAkldgasVYELLDTEKVTFTAGVPTVWLMLLQYMEKEGLKLPHLKMVVCGGSAMPRSMIKAFEDM 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 326 GFAVTHLYGLTETYGPAVVNEWHGEWDDLAKGERSAKKARQGVRYAALEgLTVMDPETmQATPADGETIGEVMFRGNIVM 405
Cdd:PRK06018 318 GVEVRHAWGMTEMSPLGTLAALKPPFSKLPGDARLDVLQKQGYPPFGVE-MKITDDAG-KELPWDGKTFGRLKVRGPAVA 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 406 KGYLKnrkASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWG 484
Cdd:PRK06018 396 AAYYR---VDGEILdDDGFFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWD 472
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 499217198 485 EVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKIQKFRLREMAK 544
Cdd:PRK06018 473 ERPLLIVQLKPGETATREEILKYMDGKIAKWWMPDDVAFVDaIPHTATGKILKTALREQFK 533
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
17-544 |
2.85e-89 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 284.68 E-value: 2.85e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 17 QPLTPLTYLERAAKTYPDhIAIIHGR-----QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPM 91
Cdd:PRK07008 8 MPLLISSLIAHAARHAGD-TEIVSRRvegdiHRYTYRDCERRAKQLAQALAALGVEPGDRVGTLAWNGYRHLEAYYGVSG 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 92 TKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQaldLAKVTPLVidyDDPDYAADAPYPKGERIGALDYEDF 171
Cdd:PRK07008 87 SGAVCHTINPRLFPEQIAYIVNHAEDRYVLFDLTFLPLVDA---LAPQCPNV---KGWVAMTDAAHLPAGSTPLLCYETL 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 172 VAGGDEDFAWsmPD-DEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHA--VYLWTLPMFHCNGWCFPW 248
Cdd:PRK07008 161 VGAQDGDYDW--PRfDENQASSLCYTSGTTGNPKGALYSHRSTVLHAYGAALPDAMGLSArdAVLPVVPMFHVNAWGLPY 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 249 TLAVQAGthvclRWVRPKP------IYDAIADHGVTHLCGAPVVMSVLINARDEDK---RTFAQTVTFNTAAappPEAVL 319
Cdd:PRK07008 239 SAPLTGA-----KLVLPGPdldgksLYELIEAERVTFSAGVPTVWLGLLNHMREAGlrfSTLRRTVIGGSAC---PPAMI 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 320 SGMADA-GFAVTHLYGLTETYGPAVVNEWHGEWDDLAKGERSAKKARQGvryAALEG--LTVMDPETmQATPADGETIGE 396
Cdd:PRK07008 311 RTFEDEyGVEVIHAWGMTEMSPLGTLCKLKWKHSQLPLDEQRKLLEKQG---RVIYGvdMKIVGDDG-RELPWDGKAFGD 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 397 VMFRGNIVMKGYLKNrkaSDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVV 476
Cdd:PRK07008 387 LQVRGPWVIDRYFRG---DASPLVDGWFPTGDVATIDADGFMQITDRSKDVIKSGGEWISSIDIENVAVAHPAVAEAACI 463
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 499217198 477 ARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLREMAK 544
Cdd:PRK07008 464 ACAHPKWDERPLLVVVKRPGAEVTREELLAFYEGKVAKWWIPDDVVFvDAIPHTATGKLQKLKLREQFR 532
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
25-542 |
1.60e-87 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 279.48 E-value: 1.60e-87
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLD 104
Cdd:PRK07656 11 LARAARRFGDKEAYVFGDQRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLNTRYT 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 105 AAVIAFQLDHAETKVLIVDREFSGVVRQALD-LAKVTPLVIDYDDPDyaadapypKGERIGALDYEDFVAGGDEDF--AW 181
Cdd:PRK07656 91 ADEAAYILARGDAKALFVLGLFLGVDYSATTrLPALEHVVICETEED--------DPHTEKMKTFTDFLAAGDPAEraPE 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 182 SMPDDEWDAIslnYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAG-THVCL 260
Cdd:PRK07656 163 VDPDDVADIL---FTSGTTGRPKGAMLTHRQLLSNAADWAEYLGLTEGDRYLAANPFFHVFGYKAGVNAPLMRGaTILPL 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 261 RWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADA-GFA-VTHLYGLTET 338
Cdd:PRK07656 240 PVFDPDEVFRLIETERITVLPGPPTMYNSLLQHPDRSAEDLSSLRLAVTGAASMPVALLERFESElGVDiVLTGYGLSEA 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 339 YGPAVVNEWHGEWDDLAkgeRSAKKARQGVRyaalegLTVMDPETMQAtpADGETiGEVMFRGNIVMKGYLKNRKASDEA 418
Cdd:PRK07656 320 SGVTTFNRLDDDRKTVA---GTIGTAIAGVE------NKIVNELGEEV--PVGEV-GELLVRGPNVMKGYYDDPEATAAA 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 419 F-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGK 497
Cdd:PRK07656 388 IdADGWLHTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVIGVPDERLGEVGKAYVVLKPGA 467
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 499217198 498 TASEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLREM 542
Cdd:PRK07656 468 ELTEEELIAYCREHLAKYKVPRSIEFlDELPKNATGKVLKRALREK 513
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
42-540 |
9.63e-83 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 264.15 E-value: 9.63e-83
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 42 RQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLI 121
Cdd:cd05934 1 GRRWTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 122 VDrefsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvaggdedfawsmpddewdAISLNYTSGTTG 201
Cdd:cd05934 81 VD------------------------------------------------------------------PASILYTSGTTG 94
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 202 NPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAG-THVCLRWVRPKPIYDAIADHGVTHL 280
Cdd:cd05934 95 PPKGVVITHANLTFAGYYSARRFGLGEDDVYLTVLPLFHINAQAVSVLAALSVGaTLVLLPRFSASRFWSDVRRYGATVT 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 281 CGAPVVMSVL----INARDEDKRtfaqtVTFNTAAAPPPEAVLSGMADAGFAVTHLYGLTETyGPAVVNEwhgewDDLAK 356
Cdd:cd05934 175 NYLGAMLSYLlaqpPSPDDRAHR-----LRAAYGAPNPPELHEEFEERFGVRLLEGYGMTET-IVGVIGP-----RDEPR 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 357 GERSAKKARQGVRYAalegltVMDPETMQAtpADGETiGEVMFR---GNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMH 433
Cdd:cd05934 244 RPGSIGRPAPGYEVR------IVDDDGQEL--PAGEP-GELVIRglrGWGFFKGYYNMPEATAEAMRNGWFHTGDLGYRD 314
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 434 PDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLA 513
Cdd:cd05934 315 ADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPEELFAFCEGQLA 394
|
490 500
....*....|....*....|....*...
gi 499217198 514 RFKVPKAVIFA-EIPKTSTGKIQKFRLR 540
Cdd:cd05934 395 YFKVPRYIRFVdDLPKTPTEKVAKAQLR 422
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
24-544 |
1.05e-81 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 263.64 E-value: 1.05e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 24 YLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKR-GIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTR 102
Cdd:PRK06839 7 WIEKRAYLHPDRIAIITEEEEMTYKQLHEYVSKVAAYLIYElNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIR 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 103 LDAAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVidyddpDYAADAPYPKGERIGALDyedfvagGDEDfaws 182
Cdd:PRK06839 87 LTENELIFQLKDSGTTVLFVEKTFQNMALSMQKVSYVQRVI------SITSLKEIEDRKIDNFVE-------KNES---- 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 183 mpddewDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNG---WCFPWTLAvqAGTHVC 259
Cdd:PRK06839 150 ------ASFIICYTSGTTGKPKGAVLTQENMFWNALNNTFAIDLTMHDRSIVLLPLFHIGGiglFAFPTLFA--GGVIIV 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 260 LRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADAGFAVTHLYGLTETy 339
Cdd:PRK06839 222 PRKFEPTKALSMIEKHKVTVVMGVPTIHQALINCSKFETTNLQSVRWFYNGGAPCPEELMREFIDRGFLFGQGFGMTET- 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 340 GPAVVNewhgewddLAKgERSAKKARQGVRYAALEGLTVMDPETMQATPadGEtIGEVMFRGNIVMKGYLKNRKASDEAF 419
Cdd:PRK06839 301 SPTVFM--------LSE-EDARRKVGSIGKPVLFCDYELIDENKNKVEV--GE-VGELLIRGPNVMKEYWNRPDATEETI 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 420 AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTA 499
Cdd:PRK06839 369 QDGWLCTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIPIAFIVKKSSSVL 448
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 499217198 500 SEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLREMAK 544
Cdd:PRK06839 449 IEKDVIEHCRLFLAKYKIPKEIVFlKELPKNATGKIQKAQLVNQLK 494
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
25-543 |
1.65e-80 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 262.36 E-value: 1.65e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIH-----GRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVpmTK--AVLH 97
Cdd:COG0365 15 LDRHAEGRGDKVALIWegedgEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAMLAC--ARigAVHS 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 98 SLNTRLDAAVIAFQLDHAETKVLIVDREF---------SGVVRQALDLAKVTPLVIDYDDPDYAADAPypkgeriGALDY 168
Cdd:COG0365 93 PVFPGFGAEALADRIEDAEAKVLITADGGlrggkvidlKEKVDEALEELPSLEHVIVVGRTGADVPME-------GDLDW 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 169 EDFVAGGDEDF-AWSMPDDewDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNT-IHAGMAKHAVYLWTLP----MFHCN 242
Cdd:COG0365 166 DELLAAASAEFePEPTDAD--DPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAkYVLDLKPGDVFWCTADigwaTGHSY 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 243 GWCFPWTlavqAGTHVCL---RWVRPKP--IYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQT---VTFNTAAAPP 314
Cdd:COG0365 244 IVYGPLL----NGATVVLyegRPDFPDPgrLWELIEKYGVTVFFTAPTAIRALMKAGDEPLKKYDLSslrLLGSAGEPLN 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 315 PEAVLSGMADAGFAVTHLYGLTETYGPAVVNEWhgeWDDLAKGerSAKKARQGVRyaalegLTVMDPEtmqATPADGETI 394
Cdd:COG0365 320 PEVWEWWYEAVGVPIVDGWGQTETGGIFISNLP---GLPVKPG--SMGKPVPGYD------VAVVDED---GNPVPPGEE 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 395 GEVMFRGNI--VMKGYLKNRKASDEAFAG---GWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPS 469
Cdd:COG0365 386 GELVIKGPWpgMFRGYWNDPERYRETYFGrfpGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPA 465
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 499217198 470 VASCGVVARHDDKWGEVPVAYVELKPGKTASEA---EIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLREMA 543
Cdd:COG0365 466 VAEAAVVGVPDEIRGQVVKAFVVLKPGVEPSDElakELQAHVREELGPYAYPREIEFvDELPKTRSGKIMRRLLRKIA 543
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
33-541 |
8.18e-80 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 258.78 E-value: 8.18e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 33 PDHIAIIHGRQR--ISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAF 110
Cdd:cd05926 1 PDAPALVVPGSTpaLTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEF 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 111 QLDHAETKVLIVDREFSGvvrQALDLAKVTPLVIDYDDPDYAADAPYPKGERIGALDyEDFVAGGDEDFAwsMPDDewDA 190
Cdd:cd05926 81 YLADLGSKLVLTPKGELG---PASRAASKLGLAILELALDVGVLIRAPSAESLSNLL-ADKKNAKSEGVP--LPDD--LA 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 191 ISLnYTSGTTGNPKGVVYHHRG-AALMAYTNTIHAGMAKHAVYLwTLPMFHCNGW---CFPwTLAVQaGTHVCLRWVRPK 266
Cdd:cd05926 153 LIL-HTSGTTGRPKGVPLTHRNlAASATNITNTYKLTPDDRTLV-VMPLFHVHGLvasLLS-TLAAG-GSVVLPPRFSAS 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 267 PIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTF-NTAAAPPPEAVLSGMaDAGFAVTHL--YGLTETYGPAV 343
Cdd:cd05926 229 TFWPDVRDYNATWYTAVPTIHQILLNRPEPNPESPPPKLRFiRSCSASLPPAVLEAL-EATFGAPVLeaYGMTEAAHQMT 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 344 VNEwhgewddLAKGER---SAKKArQGVRyaalegLTVMDPETMQATPADgetIGEVMFRGNIVMKGYLKNRKAS-DEAF 419
Cdd:cd05926 308 SNP-------LPPGPRkpgSVGKP-VGVE------VRILDEDGEILPPGV---VGEICLRGPNVTRGYLNNPEANaEAAF 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 420 AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTA 499
Cdd:cd05926 371 KDGWFRTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVLREGASV 450
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 499217198 500 SEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLRE 541
Cdd:cd05926 451 TEEELRAFCRKHLAAFKVPKKVYFvDELPKTATGKIQRRKVAE 493
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
27-541 |
2.13e-76 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 249.49 E-value: 2.13e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 27 RAAKTyPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAA 106
Cdd:PRK03640 11 RAFLT-PDRTAIEFEEKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSRE 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 107 VIAFQLDHAETKVLIVDREFsgvvrqaldLAKVTPlvidyddpdyaadapypkGERIgalDYEDFVAGGDEDFAWSMPDD 186
Cdd:PRK03640 90 ELLWQLDDAEVKCLITDDDF---------EAKLIP------------------GISV---KFAELMNGPKEEAEIQEEFD 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 187 EWDAISLNYTSGTTGNPKGVV--YHHRGAAlmAYTNTIHAGMAKHAVYLWTLPMFHCNGwcfpwtLA-----VQAGTHVC 259
Cdd:PRK03640 140 LDEVATIMYTSGTTGKPKGVIqtYGNHWWS--AVGSALNLGLTEDDCWLAAVPIFHISG------LSilmrsVIYGMRVV 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 260 L-RWVRPKPIYDAIADHGVTHLCGAPVVMSVLInARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADAGFAVTHLYGLTET 338
Cdd:PRK03640 212 LvEKFDAEKINKLLQTGGVTIISVVSTMLQRLL-ERLGEGTYPSSFRCMLLGGGPAPKPLLEQCKEKGIPVYQSYGMTET 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 339 YGPAVVNEWhgewDDLAKGERSAKKARQGVRyaalegLTVMDpETMQATPADgetIGEVMFRGNIVMKGYLKNRKASDEA 418
Cdd:PRK03640 291 ASQIVTLSP----EDALTKLGSAGKPLFPCE------LKIEK-DGVVVPPFE---EGEIVVKGPNVTKGYLNREDATRET 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 419 FAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELkpGKT 498
Cdd:PRK03640 357 FQDGWFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQVPVAFVVK--SGE 434
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 499217198 499 ASEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLRE 541
Cdd:PRK03640 435 VTEEELRHFCEEKLAKYKVPKRFYFVeELPRNASGKLLRHELKQ 478
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
29-541 |
8.96e-76 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 248.26 E-value: 8.96e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 29 AKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVI 108
Cdd:PRK06145 12 ARRTPDRAALVYRDQEISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPINYRLAADEV 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 109 AFQLDHAETKVLIVDREFSGVVrqaldlAKVTPLVIdyddpdyaadapypkgerIGALDYEDF--VAGGDEDFAWSMPDD 186
Cdd:PRK06145 92 AYILGDAGAKLLLVDEEFDAIV------ALETPKIV------------------IDAAAQADSrrLAQGGLEIPPQAAVA 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 187 EWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPwTLAV--QAGTHVCLRWVR 264
Cdd:PRK06145 148 PTDLVRLMYTSGTTDRPKGVMHSYGNLHWKSIDHVIALGLTASERLLVVGPLYHVGAFDLP-GIAVlwVGGTLRIHREFD 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 265 PKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMAD--AGFAVTHLYGLTETYGPA 342
Cdd:PRK06145 227 PEAVLAAIERHRLTCAWMAPVMLSRVLTVPDRDRFDLDSLAWCIGGGEKTPESRIRDFTRvfTRARYIDAYGLTETCSGD 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 343 VVNEWHGEWDDLAkgerSAKKARQGVRYAALEGLTVMDPETMQatpadgetiGEVMFRGNIVMKGYLKNRKASDEAFAGG 422
Cdd:PRK06145 307 TLMEAGREIEKIG----STGRALAHVEIRIADGAGRWLPPNMK---------GEICMRGPKVTKGYWKDPEKTAEAFYGD 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 423 WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEA 502
Cdd:PRK06145 374 WFRSGDVGYLDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVVLNPGATLTLE 453
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 499217198 503 EIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLRE 541
Cdd:PRK06145 454 ALDRHCRQRLASFKVPRQLKVrDELPRNPSGKVLKRVLRD 493
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
25-543 |
3.52e-74 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 245.84 E-value: 3.52e-74
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAII--HGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTR 102
Cdd:PRK12583 24 FDATVARFPDREALVvrHQALRYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVNINPA 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 103 LDAAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVIDYDDPDYAADAPYPKG-------ERIGALDYEDFVAGG 175
Cdd:PRK12583 104 YRASELEYALGQSGVRWVICADAFKTSDYHAMLQELLPGLAEGQPGALACERLPELRGvvslapaPPPGFLAWHELQARG 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 176 D----ED-FAWSMPDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTL 250
Cdd:PRK12583 184 EtvsrEAlAERQASLDRDDPINIQYTSGTTGFPKGATLSHHNILNNGYFVAESLGLTEHDRLCVPVPLYHCFGMVLANLG 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 251 AVQAGThvCLrwVRPKPIYD------AIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAP-PPEAVLSGMA 323
Cdd:PRK12583 264 CMTVGA--CL--VYPNEAFDplatlqAVEEERCTALYGVPTMFIAELDHPQRGNFDLSSLRTGIMAGAPcPIEVMRRVMD 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 324 DAGFA-VTHLYGLTETygpAVVNEWHGEWDDLAKGERSAKKA--RQGVRyaalegltVMDPETmqATPADGEtIGEVMFR 400
Cdd:PRK12583 340 EMHMAeVQIAYGMTET---SPVSLQTTAADDLERRVETVGRTqpHLEVK--------VVDPDG--ATVPRGE-IGELCTR 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 401 GNIVMKGYLKNRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARH 479
Cdd:PRK12583 406 GYSVMKGYWNNPEATAESIdEDGWMHTGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVADVQVFGVP 485
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 499217198 480 DDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLREMA 543
Cdd:PRK12583 486 DEKYGEEIVAWVRLHPGHAASEEELREFCKARIAHFKVPRYFRFVdEFPMTVTGKVQKFRMREIS 550
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
43-535 |
1.38e-73 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 242.12 E-value: 1.38e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 43 QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIV 122
Cdd:cd05911 9 KELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVIFT 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 123 DREFSGVVRQALDLAKVTPLVIDYDDpdyaadapypKGERIGAL-DYEDFVAGG-DEDFAWSMPDDEWDAISLNYTSGTT 200
Cdd:cd05911 89 DPDGLEKVKEAAKELGPKDKIIVLDD----------KPDGVLSIeDLLSPTLGEeDEDLPPPLKDGKDDTAAILYSSGTT 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 201 GNPKGVVYHHRG--AALMAYTNTIHAGMAKHAVYLWTLPMFHcnGWCFPWTLA--VQAGTHVCLRwvRPKPIY--DAIAD 274
Cdd:cd05911 159 GLPKGVCLSHRNliANLSQVQTFLYGNDGSNDVILGFLPLYH--IYGLFTTLAslLNGATVIIMP--KFDSELflDLIEK 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 275 HGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMA--DAGFAVTHLYGLTETYGPAVVNEWHGEWD 352
Cdd:cd05911 235 YKITFLYLVPPIAAALAKSPLLDKYDLSSLRVILSGGAPLSKELQELLAkrFPNATIKQGYGMTETGGILTVNPDGDDKP 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 353 DlakgerSAKKARQGVRyaalegLTVMDPETMQATPADGEtiGEVMFRGNIVMKGYLKNRKASDEAF-AGGWFHSGDLGV 431
Cdd:cd05911 315 G------SVGRLLPNVE------AKIVDDDGKDSLGPNEP--GEICVRGPQVMKGYYNNPEATKETFdEDGWLHTGDIGY 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 432 MHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRAL 511
Cdd:cd05911 381 FDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKPGEKLTEKEVKDYVAKK 460
|
490 500
....*....|....*....|....*.
gi 499217198 512 LARFK-VPKAVIFA-EIPKTSTGKIQ 535
Cdd:cd05911 461 VASYKqLRGGVVFVdEIPKSASGKIL 486
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
17-544 |
8.56e-72 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 239.33 E-value: 8.56e-72
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 17 QPLTPLT---YLERAAKTYPDHIAII--HGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPM 91
Cdd:PRK08315 11 VPLLEQTigqLLDRTAARYPDREALVyrDQGLRWTYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFATAK 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 92 TKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREFSGV----------------VRQALDLAKVTPL--VIDYDDPDYAa 153
Cdd:PRK08315 91 IGAILVTINPAYRLSELEYALNQSGCKALIAADGFKDSdyvamlyelapelatcEPGQLQSARLPELrrVIFLGDEKHP- 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 154 dapypkgeriGALDYEDFVAGGDEDfawsmPDDE----------WDAISLNYTSGTTGNPKGVVYHHRG--------AAL 215
Cdd:PRK08315 170 ----------GMLNFDELLALGRAV-----DDAElaarqatldpDDPINIQYTSGTTGFPKGATLTHRNilnngyfiGEA 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 216 MAYTNT----IHagmakhavylwtLPMFHCNGwCFPWTLAvqAGTH-VCLrwVRPKPIYD------AIADHGVTHLCGAP 284
Cdd:PRK08315 235 MKLTEEdrlcIP------------VPLYHCFG-MVLGNLA--CVTHgATM--VYPGEGFDplatlaAVEEERCTALYGVP 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 285 VVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLS------GMADagfaVTHLYGLTETyGPavVNEWHGEWDDLAKge 358
Cdd:PRK08315 298 TMFIAELDHPDFARFDLSSLRTGIMAGSPCPIEVMKrvidkmHMSE----VTIAYGMTET-SP--VSTQTRTDDPLEK-- 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 359 RSAKKARqgvryaALEGLTV--MDPETMQATPAdGETiGEVMFRGNIVMKGYLKNRKASDEAF-AGGWFHSGDLGVMHPD 435
Cdd:PRK08315 369 RVTTVGR------ALPHLEVkiVDPETGETVPR-GEQ-GELCTRGYSVMKGYWNDPEKTAEAIdADGWMHTGDLAVMDEE 440
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 436 GYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARF 515
Cdd:PRK08315 441 GYVNIVGRIKDMIIRGGENIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGATLTEEDVRDFCRGKIAHY 520
|
570 580 590
....*....|....*....|....*....|
gi 499217198 516 KVPKAVIFA-EIPKTSTGKIQKFRLREMAK 544
Cdd:PRK08315 521 KIPRYIRFVdEFPMTVTGKIQKFKMREMMI 550
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
25-541 |
2.23e-71 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 237.75 E-value: 2.23e-71
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLD 104
Cdd:PRK07786 23 LARHALMQPDAPALRFLGNTTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLT 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 105 AAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVIDYDDPDYAadapypkgeriGALDYEDFVAGGDEDFAW-SM 183
Cdd:PRK07786 103 PPEIAFLVSDCGAHVVVTEAALAPVATAVRDIVPLLSTVVVAGGSSDD-----------SVLGYEDLLAEAGPAHAPvDI 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 184 PDDEWDAISlnYTSGTTGNPKGVVYHH---RGAALMA-YTNTIHAGmakHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVC 259
Cdd:PRK07786 172 PNDSPALIM--YTSGTTGRPKGAVLTHanlTGQAMTClRTNGADIN---SDVGFVGVPLFHIAGIGSMLPGLLLGAPTVI 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 260 --LRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVtFNTAAAPPPEAVLSGMADA--GFAVTHLYGL 335
Cdd:PRK07786 247 ypLGAFDPGQLLDVLEAEKVTGIFLVPAQWQAVCAEQQARPRDLALRV-LSWGAAPASDTLLRQMAATfpEAQILAAFGQ 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 336 TETyGPAVVnewhgewddLAKGERSAKKARQGVRYAALEGLTVMDpETMQATPAdGEtIGEVMFRGNIVMKGYLKNRKAS 415
Cdd:PRK07786 326 TEM-SPVTC---------MLLGEDAIRKLGSVGKVIPTVAARVVD-ENMNDVPV-GE-VGEIVYRAPTLMSGYWNNPEAT 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 416 DEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKP 495
Cdd:PRK07786 393 AEAFAGGWFHSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAAVRN 472
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 499217198 496 GKTASE-AEIIEHCRALLARFKVPKAV-IFAEIPKTSTGKIQKFRLRE 541
Cdd:PRK07786 473 DDAALTlEDLAEFLTDRLARYKHPKALeIVDALPRNPAGKVLKTELRE 520
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
20-544 |
1.49e-70 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 235.72 E-value: 1.49e-70
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 20 TPLTYLERAAKTYPDHIAIIHGR------QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTppmleAHFgvpmtk 93
Cdd:PRK13295 25 TINDDLDACVASCPDKTAVTAVRlgtgapRRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNW-----WEF------ 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 94 AVLHSLNTRLDAAV-----------IAFQLDHAETKVLIVDREFSGV--------VRQAL-DLAKVtpLVIDyddpdyaa 153
Cdd:PRK13295 94 TVLYLACSRIGAVLnplmpifrereLSFMLKHAESKVLVVPKTFRGFdhaamarrLRPELpALRHV--VVVG-------- 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 154 dapypkGEriGALDYEDFVAGGDED--------FAWSMPDDEwDAISLNYTSGTTGNPKGVVYHHRG--AALMAYTNTIH 223
Cdd:PRK13295 164 ------GD--GADSFEALLITPAWEqepdapaiLARLRPGPD-DVTQLIYTSGTTGEPKGVMHTANTlmANIVPYAERLG 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 224 AGmAKHaVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLR--WvRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTF 301
Cdd:PRK13295 235 LG-ADD-VILMASPMAHQTGFMYGLMMPVMLGATAVLQdiW-DPARAAELIRTEGVTFTMASTPFLTDLTRAVKESGRPV 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 302 AQTVTFNTAAAPPPEAVLSGMADA-GFAVTHLYGLTETygpAVVNEWHGEWDDlakgERSAkkARQGVRYAALEgLTVMD 380
Cdd:PRK13295 312 SSLRTFLCAGAPIPGALVERARAAlGAKIVSAWGMTEN---GAVTLTKLDDPD----ERAS--TTDGCPLPGVE-VRVVD 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 381 PETmQATPADgeTIGEVMFRGNIVMKGYLK----NRKASDeafagGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENIS 456
Cdd:PRK13295 382 ADG-APLPAG--QIGRLQVRGCSNFGGYLKrpqlNGTDAD-----GWFDTGDLARIDADGYIRISGRSKDVIIRGGENIP 453
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 457 SIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRAL-LARFKVP-KAVIFAEIPKTSTGKI 534
Cdd:PRK13295 454 VVEIEALLYRHPAIAQVAIVAYPDERLGERACAFVVPRPGQSLDFEEMVEFLKAQkVAKQYIPeRLVVRDALPRTPSGKI 533
|
570
....*....|
gi 499217198 535 QKFRLREMAK 544
Cdd:PRK13295 534 QKFRLREMLR 543
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
23-543 |
1.45e-68 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 232.54 E-value: 1.45e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 23 TY--LERAAKTYPDHIAII--------HGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPpmlEAHFGV--P 90
Cdd:PRK07529 27 TYelLSRAAARHPDAPALSflldadplDRPETWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLP---ETHFALwgG 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 91 MTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREFSG---------VVRQALDLAKVtpLVIDYDDPDYAADAPYPKGE 161
Cdd:PRK07529 104 EAAGIANPINPLLEPEQIAELLRAAGAKVLVTLGPFPGtdiwqkvaeVLAALPELRTV--VEVDLARYLPGPKRLAVPLI 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 162 R----IGALDYEDFVAGGDEDFAWSMPDDEWDAI-SLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTL 236
Cdd:PRK07529 182 RrkahARILDFDAELARQPGDRLFSGRPIGPDDVaAYFHTGGTTGMPKLAQHTHGNEVANAWLGALLLGLGPGDTVFCGL 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 237 PMFHCNGwCFPWTLA-VQAGTHVclrwVRPKP-----------IYDAIADHGVTHLCGAPVVMSVL----INARDEDKRT 300
Cdd:PRK07529 262 PLFHVNA-LLVTGLApLARGAHV----VLATPqgyrgpgvianFWKIVERYRINFLSGVPTVYAALlqvpVDGHDISSLR 336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 301 FAQTvtfntAAAPPPEAVLSGMADA-GFAVTHLYGLTETYGPAVVNEWHGEWDDLAKGERSAkkaRQGVRYAalegltVM 379
Cdd:PRK07529 337 YALC-----GAAPLPVEVFRRFEAAtGVRIVEGYGLTEATCVSSVNPPDGERRIGSVGLRLP---YQRVRVV------IL 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 380 DPETMQATPADGETIGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIE 459
Cdd:PRK07529 403 DDAGRYLRDCAVDEVGVLCIAGPNVFSGYLEAAHNKGLWLEDGWLNTGDLGRIDADGYFWLTGRAKDLIIRGGHNIDPAA 482
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 460 VEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLA-RFKVPKAV-IFAEIPKTSTGKIQKF 537
Cdd:PRK07529 483 IEEALLRHPAVALAAAVGRPDAHAGELPVAYVQLKPGASATEAELLAFARDHIAeRAAVPKHVrILDALPKTAVGKIFKP 562
|
....*.
gi 499217198 538 RLREMA 543
Cdd:PRK07529 563 ALRRDA 568
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
16-541 |
1.66e-68 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 229.87 E-value: 1.66e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 16 HQPLTPLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAhFGVPMTKAV 95
Cdd:PRK06188 9 HSGATYGHLLVSALKRYPDRPALVLGDTRLTYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLMA-IGAAQLAGL 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 96 ----LHSLNTRLDAaviAFQLDHAETKVLIVD-REFsgVVRQALDLAKVTPLvidyddpdyaadapyPKGERIGALDY-E 169
Cdd:PRK06188 88 rrtaLHPLGSLDDH---AYVLEDAGISTLIVDpAPF--VERALALLARVPSL---------------KHVLTLGPVPDgV 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 170 DFVAGGD--EDFAWSMPDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFP 247
Cdd:PRK06188 148 DLLAAAAkfGPAPLVAAALPPDIAGLAYTGGTTGKPKGVMGTHRSIATMAQIQLAEWEWPADPRFLMCTPLSHAGGAFFL 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 248 WTLaVQAGTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFA--QTVTFNtAAAPPPEAVLSGMADA 325
Cdd:PRK06188 228 PTL-LRGGTVIVLAKFDPAEVLRAIEEQRITATFLVPTMIYALLDHPDLRTRDLSslETVYYG-ASPMSPVRLAEAIERF 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 326 GFAVTHLYGLTETYGPAVVnewhgewddLAKGERSAKKARQ----GVRYAALEgLTVMDPEtMQATPAdGEtIGEVMFRG 401
Cdd:PRK06188 306 GPIFAQYYGQTEAPMVITY---------LRKRDHDPDDPKRltscGRPTPGLR-VALLDED-GREVAQ-GE-VGEICVRG 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 402 NIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDD 481
Cdd:PRK06188 373 PLVMDGYWNRPEETAEAFRDGWLHTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFPREVEDVLAEHPAVAQVAVIGVPDE 452
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 499217198 482 KWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKIQKFRLRE 541
Cdd:PRK06188 453 KWGEAVTAVVVLRPGAAVDAAELQAHVKERKGSVHAPKQVDFVDsLPLTALGKPDKKALRA 513
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
44-541 |
4.55e-68 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 226.11 E-value: 4.55e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 44 RISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVD 123
Cdd:cd05903 1 RLTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVVP 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 124 REFsgvvrqaldlakvtplvidyddpdyaadapypkgeriGALDYEDfvaggdedfawsMPDDewdAISLNYTSGTTGNP 203
Cdd:cd05903 81 ERF-------------------------------------RQFDPAA------------MPDA---VALLLFTSGTTGEP 108
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 204 KGVVYHHrgaalmaytNTIHAGMAKHA---------VYLWTLPMFHCNGWCFPWTLAVQAGT--HVCLRWvRPKPIYDAI 272
Cdd:cd05903 109 KGVMHSH---------NTLSASIRQYAerlglgpgdVFLVASPMAHQTGFVYGFTLPLLLGApvVLQDIW-DPDKALALM 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 273 ADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADAGFA-VTHLYGLTETYGpAVVNEwhgEW 351
Cdd:cd05903 179 REHGVTFMMGATPFLTDLLNAVEEAGEPLSRLRTFVCGGATVPRSLARRAAELLGAkVCSAYGSTECPG-AVTSI---TP 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 352 DDLAKGERSAKKARQGVRyaalegLTVMDPEtmQATPADGEtIGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGV 431
Cdd:cd05903 255 APEDRRLYTDGRPLPGVE------IKVVDDT--GATLAPGV-EGELLSRGPSVFLGYLDRPDLTADAAPEGWFRTGDLAR 325
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 432 MHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHC-RA 510
Cdd:cd05903 326 LDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGALLTFDELVAYLdRQ 405
|
490 500 510
....*....|....*....|....*....|..
gi 499217198 511 LLARFKVP-KAVIFAEIPKTSTGKIQKFRLRE 541
Cdd:cd05903 406 GVAKQYWPeRLVHVDDLPRTPSGKVQKFRLRE 437
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
189-540 |
4.83e-68 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 223.31 E-value: 4.83e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 DAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVclrwVRPKPI 268
Cdd:cd05917 3 DVINIQFTSGTTGSPKGATLTHHNIVNNGYFIGERLGLTEQDRLCIPVPLFHCFGSVLGVLACLTHGATM----VFPSPS 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 269 YD------AIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLS------GMADagfaVTHLYGLT 336
Cdd:cd05917 79 FDplavleAIEKEKCTALHGVPTMFIAELEHPDFDKFDLSSLRTGIMAGAPCPPELMKrvievmNMKD----VTIAYGMT 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 337 ETygpAVVNEWHGEWDDLAKGERSAKKARQGVRyaalegLTVMDPETmQATPADGETiGEVMFRGNIVMKGYLKNRKASD 416
Cdd:cd05917 155 ET---SPVSTQTRTDDSIEKRVNTVGRIMPHTE------AKIVDPEG-GIVPPVGVP-GELCIRGYSVMKGYWNDPEKTA 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 417 EAFAG-GWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKP 495
Cdd:cd05917 224 EAIDGdGWLHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEVCAWIRLKE 303
|
330 340 350 360
....*....|....*....|....*....|....*....|....*.
gi 499217198 496 GKTASEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLR 540
Cdd:cd05917 304 GAELTEEDIKAYCKGKIAHYKVPRYVFFVdEFPLTVSGKIQKFKLR 349
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
16-546 |
7.39e-67 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 225.99 E-value: 7.39e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 16 HQPLTPLTY-LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQ-KRGIGKGDTVTVMLSNTPPMLEAHFGVPMTK 93
Cdd:PRK08314 6 TLPETSLFHnLEVSARRYPDKTAIVFYGRAISYRELLEEAERLAGYLQqECGVRKGDRVLLYMQNSPQFVIAYYAILRAN 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 94 AVLHSLNTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQA---LDLAKV------------TPLVIDYDDPDYAADAPYP 158
Cdd:PRK08314 86 AVVVPVNPMNREEELAHYVTDSGARVAIVGSELAPKVAPAvgnLRLRHVivaqysdylpaePEIAVPAWLRAEPPLQALA 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 159 KGeriGALDYEDFVAGGDEDFAWSM-PDDEwdAIsLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLP 237
Cdd:PRK08314 166 PG---GVVAWKEALAAGLAPPPHTAgPDDL--AV-LPYTSGTTGVPKGCMHTHRTVMANAVGSVLWSNSTPESVVLAVLP 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 238 MFHCNGWCFPWTLAVQAGTHVCL--RWVRpKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPP 315
Cdd:PRK08314 240 LFHVTGMVHSMNAPIYAGATVVLmpRWDR-EAAARLIERYRVTHWTNIPTMVVDFLASPGLAERDLSSLRYIGGGGAAMP 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 316 EAVLSGMADAgFAVTHL--YGLTETYGPAVVNEWHgewddlakgerSAKKARQGVRYAALEGLtVMDPETMQATPaDGEt 393
Cdd:PRK08314 319 EAVAERLKEL-TGLDYVegYGLTETMAQTHSNPPD-----------RPKLQCLGIPTFGVDAR-VIDPETLEELP-PGE- 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 394 IGEVMFRGNIVMKGYLKNRKASDEAFA--GG--WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPS 469
Cdd:PRK08314 384 VGEIVVHGPQVFKGYWNRPEATAEAFIeiDGkrFFRTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPA 463
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 470 VASCGVVARHDDKWGEVPVAYVELKPGK--TASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKIQkFRL---REMA 543
Cdd:PRK08314 464 IQEACVIATPDPRRGETVKAVVVLRPEArgKTTEEEIIAWAREHMAAYKYPRIVEFVDsLPKSGSGKIL-WRQlqeQEKA 542
|
...
gi 499217198 544 KLA 546
Cdd:PRK08314 543 RAA 545
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
15-534 |
1.60e-66 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 224.04 E-value: 1.60e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 15 NHQPLTPLTYLerAAKTYPDHIAIIHGR--QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMT 92
Cdd:cd05904 3 TDLPLDSVSFL--FASAHPSRPALIDAAtgRALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 93 KAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREfsgVVRQALDLAkvTPLVIDYDdpdyaadapypkgERIGALDYEDFV 172
Cdd:cd05904 81 GAVVTTANPLSTPAEIAKQVKDSGAKLAFTTAE---LAEKLASLA--LPVVLLDS-------------AEFDSLSFSDLL 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 173 AGGDEDFAWSMPDDEWDAISLNYTSGTTGNPKGVVYHHRGaaLMAYTNTIHAGMA----KHAVYLWTLPMFHCNG--WCF 246
Cdd:cd05904 143 FEADEAEPPVVVIKQDDVAALLYSSGTTGRSKGVMLTHRN--LIAMVAQFVAGEGsnsdSEDVFLCVLPMFHIYGlsSFA 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 247 PWTLAVQAGTHVCLRWVRPKpIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADAG 326
Cdd:cd05904 221 LGLLRLGATVVVMPRFDLEE-LLAAIERYKVTHLPVVPPIVLALVKSPIVDKYDLSSLRQIMSGAAPLGKELIEAFRAKF 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 327 FAVTHL--YGLTETYGPAVVNewhgewddLAKGERSAKKARQGVRYAALEGLTVmDPETMQATPAdGETiGEVMFRGNIV 404
Cdd:cd05904 300 PNVDLGqgYGMTESTGVVAMC--------FAPEKDRAKYGSVGRLVPNVEAKIV-DPETGESLPP-NQT-GELWIRGPSI 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 405 MKGYLKNRKASDEAFAG-GWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKW 483
Cdd:cd05904 369 MKGYLNNPEATAATIDKeGWLHTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDEEA 448
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 499217198 484 GEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKI 534
Cdd:cd05904 449 GEVPMAFVVRKPGSSLTEDEIMDFVAKQVAPYKKVRKVAFVDaIPKSPSGKI 500
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
45-541 |
3.46e-66 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 220.30 E-value: 3.46e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 45 ISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVlivdr 124
Cdd:cd05912 2 YTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLKDSDVKL----- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 125 efsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvaggdEDFAwsmpddewdaiSLNYTSGTTGNPK 204
Cdd:cd05912 77 ----------------------------------------------------DDIA-----------TIMYTSGTTGKPK 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 205 GVV-------YHHRGAALmaytntiHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLRWVRPKPIYDAIADHGV 277
Cdd:cd05912 94 GVQqtfgnhwWSAIGSAL-------NLGLTEDDNWLCALPLFHISGLSILMRSVIYGMTVYLVDKFDAEQVLHLINSGKV 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 278 THLCGAPVVMSVLInarDEDKRTFAQTV-TFNTAAAPPPEAVLSGMADAGFAVTHLYGLTETYGPAVVnewhGEWDDLAK 356
Cdd:cd05912 167 TIISVVPTMLQRLL---EILGEGYPNNLrCILLGGGPAPKPLLEQCKEKGIPVYQSYGMTETCSQIVT----LSPEDALN 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 357 GERSAKKARQGVRyaalegLTVMDPETMQatpadgETIGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDG 436
Cdd:cd05912 240 KIGSAGKPLFPVE------LKIEDDGQPP------YEVGEILLKGPNVTKGYLNRPDATEESFENGWFKTGDIGYLDEEG 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 437 YIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKpgKTASEAEIIEHCRALLARFK 516
Cdd:cd05912 308 FLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVVSE--RPISEEELIAYCSEKLAKYK 385
|
490 500
....*....|....*....|....*.
gi 499217198 517 VPKAVIFA-EIPKTSTGKIQKFRLRE 541
Cdd:cd05912 386 VPKKIYFVdELPRTASGKLLRHELKQ 411
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
23-541 |
1.23e-61 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 211.44 E-value: 1.23e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 23 TYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTR 102
Cdd:PRK07470 11 HFLRQAARRFPDRIALVWGDRSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTNFR 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 103 LDAAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVIDYddpdyaadapypkGERIGALDYEDFVAGGDEDFAWS 182
Cdd:PRK07470 91 QTPDEVAYLAEASGARAMICHADFPEHAAAVRAASPDLTHVVAI-------------GGARAGLDYEALVARHLGARVAN 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 183 MPDDEWDAISLNYTSGTTGNPK-GVVYHHRgaalMAYTNTIH-----AGMAKHAVYLWTLPMFHcngwcfpwtlavQAGT 256
Cdd:PRK07470 158 AAVDHDDPCWFFFTSGTTGRPKaAVLTHGQ----MAFVITNHladlmPGTTEQDASLVVAPLSH------------GAGI 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 257 HVCLRWVR-------------PKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPppeavlSGMA 323
Cdd:PRK07470 222 HQLCQVARgaatvllpserfdPAEVWALVERHRVTNLFTVPTILKMLVEHPAVDRYDHSSLRYVIYAGAP------MYRA 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 324 DAGFAVTHL-------YGLTETYG------PAVvnewHGEWDDLAKGERSAKKARQGVRyaalegLTVMDPEtMQATPAd 390
Cdd:PRK07470 296 DQKRALAKLgkvlvqyFGLGEVTGnitvlpPAL----HDAEDGPDARIGTCGFERTGME------VQIQDDE-GRELPP- 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 391 GETiGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSV 470
Cdd:PRK07470 364 GET-GEICVIGPAVFAGYYNNPEANAKAFRDGWFRTGDLGHLDARGFLYITGRASDMYISGGSNVYPREIEEKLLTHPAV 442
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 499217198 471 ASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLRE 541
Cdd:PRK07470 443 SEVAVLGVPDPVWGEVGVAVCVARDGAPVDEAELLAWLDGKVARYKLPKRFFFwDALPKSGYGKITKKMVRE 514
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
46-540 |
5.35e-61 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 207.19 E-value: 5.35e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 46 SYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDRE 125
Cdd:cd05972 2 SFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVTDAE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 126 fsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvaggdedfawsmpddewDAISLNYTSGTTGNPKG 205
Cdd:cd05972 82 ---------------------------------------------------------------DPALIYFTSGTTGLPKG 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 206 VVYHHR-GAALMAYTNTIHaGMAKHAVYlWTL--PMFHCNGWC---FPWTLAVQAGTHVCLRWVrPKPIYDAIADHGVTH 279
Cdd:cd05972 99 VLHTHSyPLGHIPTAAYWL-GLRPDDIH-WNIadPGWAKGAWSsffGPWLLGATVFVYEGPRFD-AERILELLERYGVTS 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 280 LCGAPVVMSVLInARDEDKRTFAQTVTFNTAAAP-PPEAVLSGMADAGFAVTHLYGLTETygPAVVNEWHgeWDDLAKGe 358
Cdd:cd05972 176 FCGPPTAYRMLI-KQDLSSYKFSHLRLVVSAGEPlNPEVIEWWRAATGLPIRDGYGQTET--GLTVGNFP--DMPVKPG- 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 359 rSAKKARQGVRYAalegltVMDPETMQATPAdgeTIGEVMFRGNIV--MKGYLKNRKASDEAFAGGWFHSGDLGVMHPDG 436
Cdd:cd05972 250 -SMGRPTPGYDVA------IIDDDGRELPPG---EEGDIAIKLPPPglFLGYVGDPEKTEASIRGDYYLTGDRAYRDEDG 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 437 YIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEA---EIIEHCRALLA 513
Cdd:cd05972 320 YFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSEElaeELQGHVKKVLA 399
|
490 500
....*....|....*....|....*...
gi 499217198 514 RFKVPKAVIF-AEIPKTSTGKIQKFRLR 540
Cdd:cd05972 400 PYKYPREIEFvEELPKTISGKIRRVELR 427
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
21-546 |
1.28e-60 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 209.90 E-value: 1.28e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 21 PLT-YLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSL 99
Cdd:PRK06178 34 PLTeYLRAWARERPQRPAIIFYGHVITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPV 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 100 NTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQ-----ALDLAKVTPLVIDYDDPDYAADAPYPKGERIGALDYEDFVAG 174
Cdd:PRK06178 114 SPLFREHELSYELNDAGAEVLLALDQLAPVVEQvraetSLRHVIVTSLADVLPAEPTLPLPDSLRAPRLAAAGAIDLLPA 193
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 175 -GDEDFAWSMPDDEWDAIS-LNYTSGTTGNPKGVVYHHRGAALMAYTNT-IHAGMAKHAVYLWTLPMFHCNGWCFPWTLA 251
Cdd:PRK06178 194 lRACTAPVPLPPPALDALAaLNYTGGTTGMPKGCEHTQRDMVYTAAAAYaVAVVGGEDSVFLSFLPEFWIAGENFGLLFP 273
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 252 VQAGTHVCL--RWvRPKPIYDAIADHGVTHlCGAPVVMSVLI----NARDEDKRTFAQT--VTFNTAAAPP-----PEAV 318
Cdd:PRK06178 274 LFSGATLVLlaRW-DAVAFMAAVERYRVTR-TVMLVDNAVELmdhpRFAEYDLSSLRQVrvVSFVKKLNPDyrqrwRALT 351
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 319 LSGMADAGfavthlYGLTETYGPAVVNEWHGEWD-DLakgeRSAkkarqgvryAALEGLTV-------MDPETMQATPAD 390
Cdd:PRK06178 352 GSVLAEAA------WGMTETHTCDTFTAGFQDDDfDL----LSQ---------PVFVGLPVpgtefkiCDFETGELLPLG 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 391 GEtiGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSV 470
Cdd:PRK06178 413 AE--GEIVVRTPSLLKGYWNKPEATAEALRDGWLHTGDIGKIDEQGFLHYLGRRKEMLKVNGMSVFPSEVEALLGQHPAV 490
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 499217198 471 ASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAEIPKTSTGKIQKFRLREMAKLA 546
Cdd:PRK06178 491 LGSAVVGRPDPDKGQVPVAFVQLKPGADLTAAALQAWCRENMAVYKVPEIRIVDALPMTATGKVRKQDLQALAEEL 566
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
44-534 |
1.29e-60 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 206.18 E-value: 1.29e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 44 RISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVD 123
Cdd:cd05935 1 SLTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVG 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 124 REFsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvaggdEDFAWsmpddewdaisLNYTSGTTGNP 203
Cdd:cd05935 81 SEL--------------------------------------------------DDLAL-----------IPYTSGTTGLP 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 204 KGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCL--RWVRpKPIYDAIADHGVTHLC 281
Cdd:cd05935 100 KGCMHTHFSAAANALQSAVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLmaRWDR-ETALELIEKYKVTFWT 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 282 GAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMAD-AGFAVTHLYGLTETYGPAVVNEWHgewddlakgerS 360
Cdd:cd05935 179 NIPTMLVDLLATPEFKTRDLSSLKVLTGGGAPMPPAVAEKLLKlTGLRFVEGYGLTETMSQTHTNPPL-----------R 247
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 361 AKKARQGVRYAALEGLtVMDPETMQATPaDGEtIGEVMFRGNIVMKGYLKNRKASDEAFA--GG--WFHSGDLGVMHPDG 436
Cdd:cd05935 248 PKLQCLGIP*FGVDAR-VIDIETGRELP-PNE-VGEIVVRGPQIFKGYWNRPEETEESFIeiKGrrFFRTGDLGYMDEEG 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 437 YIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGK--TASEAEIIEHCRALLAR 514
Cdd:cd05935 325 YFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEYrgKVTEEDIIEWAREQMAA 404
|
490 500
....*....|....*....|.
gi 499217198 515 FKVPKAVIFA-EIPKTSTGKI 534
Cdd:cd05935 405 YKYPREVEFVdELPRSASGKI 425
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
20-542 |
2.10e-60 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 209.47 E-value: 2.10e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 20 TPLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSL 99
Cdd:PRK05605 33 TLVDLYDNAVARFGDRPALDFFGATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAVVVEH 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 100 NTRLDAAVIAFQL-DHAeTKVLIVDREFSGVVRQ-----------ALDLAKVTPLVIDYDDPDYAADAPYPK----GERI 163
Cdd:PRK05605 113 NPLYTAHELEHPFeDHG-ARVAIVWDKVAPTVERlrrttpletivSVNMIAAMPLLQRLALRLPIPALRKARaaltGPAP 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 164 GALDYEDFVA---GGDEDFAwSMPDDEWDAISL-NYTSGTTGNPKGVVYHHRGaaLMAytntiHAGMAKHAV-------- 231
Cdd:PRK05605 192 GTVPWETLVDaaiGGDGSDV-SHPRPTPDDVALiLYTSGTTGKPKGAQLTHRN--LFA-----NAAQGKAWVpglgdgpe 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 232 -YLWTLPMFHCNGWCFPWTLAVQAGTHVCLRwvrPKP----IYDAIADHGVTHLCGAPVVMS-VLINARDEDKRTFAQTV 305
Cdd:PRK05605 264 rVLAALPMFHAYGLTLCLTLAVSIGGELVLL---PAPdidlILDAMKKHPPTWLPGVPPLYEkIAEAAEERGVDLSGVRN 340
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 306 TFNTAAAPPPEAVLSGMADAGFAVTHLYGLTETYGPAVVNewhgewddlakgerSAKKARQ----GVRYAALEgLTVMDP 381
Cdd:PRK05605 341 AFSGAMALPVSTVELWEKLTGGLLVEGYGLTETSPIIVGN--------------PMSDDRRpgyvGVPFPDTE-VRIVDP 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 382 ETMQATPADGETiGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVE 461
Cdd:PRK05605 406 EDPDETMPDGEE-GELLVRGPQVFKGYWNRPEETAKSFLDGWFRTGDVVVMEEDGFIRIVDRIKELIITGGFNVYPAEVE 484
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 462 DALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKA-VIFAEIPKTSTGKIQKFRLR 540
Cdd:PRK05605 485 EVLREHPGVEDAAVVGLPREDGSEEVVAAVVLEPGAALDPEGLRAYCREHLTRYKVPRRfYHVDELPRDQLGKVRRREVR 564
|
..
gi 499217198 541 EM 542
Cdd:PRK05605 565 EE 566
|
|
| menE |
TIGR01923 |
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, ... |
46-536 |
4.91e-60 |
|
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, which is involved in the fourth step of the menaquinone biosynthesis pathway. O-succinylbenzoate-CoA ligase, together with menB - naphtoate synthase, take 2-succinylbenzoate and convert it into 1,4-di-hydroxy-2- naphtoate. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone]
Pssm-ID: 162605 [Multi-domain] Cd Length: 436 Bit Score: 204.99 E-value: 4.91e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 46 SYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDRE 125
Cdd:TIGR01923 1 TWQDLDCEAAHLAKALKAQGIRSGSRVALVGQNSIEMVLLLHACLLLGAEIAMLNTRLTENERTNQLEDLDVQLLLTDSL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 126 FSGVVRQALDLAkvtplvidyddpdyaadapypkgeRIGALDYEDfvaggdEDFAWSMPDDewDAISLNYTSGTTGNPKG 205
Cdd:TIGR01923 81 LEEKDFQADSLD------------------------RIEAAGRYE------TSLSASFNMD--QIATLMFTSGTTGKPKA 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 206 VVYHHRG--AALMAYTNTIHAGmaKHAVYLWTLPMFHCNGWCFPWTLAVQAGThvcLRWVRPKP-IYDAIADHGVTHLCG 282
Cdd:TIGR01923 129 VPHTFRNhyASAVGSKENLGFT--EDDNWLLSLPLYHISGLSILFRWLIEGAT---LRIVDKFNqLLEMIANERVTHISL 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 283 APVVMSVLINARDEDKRTfaQTVTFNTAAAPPPeaVLSGMADAGFAVTHLYGLTETYGPAVVnewhgewddlAKGERSAK 362
Cdd:TIGR01923 204 VPTQLNRLLDEGGHNENL--RKILLGGSAIPAP--LIEEAQQYGLPIYLSYGMTETCSQVTT----------ATPEMLHA 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 363 KARQGVRYAALEgltvmdpetMQATPADGETIGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKD 442
Cdd:TIGR01923 270 RPDVGRPLAGRE---------IKIKVDNKEGHGEIMVKGANLMKGYLYQGELTPAFEQQGWFNTGDIGELDGEGFLYVLG 340
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 443 RSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKpgKTASEAEIIEHCRALLARFKVPKAVI 522
Cdd:TIGR01923 341 RRDDLIISGGENIYPEEIETVLYQHPGIQEAVVVPKPDAEWGQVPVAYIVSE--SDISQAKLIAYLTEKLAKYKVPIAFE 418
|
490
....*....|....*
gi 499217198 523 FA-EIPKTSTGKIQK 536
Cdd:TIGR01923 419 KLdELPYNASGKILR 433
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
24-540 |
9.37e-60 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 206.07 E-value: 9.37e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 24 YLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRL 103
Cdd:cd05959 9 VDLNLNEGRGDKTAFIDDAGSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTLL 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 104 DAAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVIDYDDPdyaadapypKGERIGALDYEDFVAGGDEDF--AW 181
Cdd:cd05959 89 TPDDYAYYLEDSRARVVVVSGELAPVLAAALTKSEHTLVVLIVSGG---------AGPEAGALLLAELVAAEAEQLkpAA 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 182 SMPDDEwdAISLnYTSGTTGNPKGVVYHHRGAALMAYT---NTIhaGMAKHAVYLWTLPMFHC----NGWCFPWTlavqA 254
Cdd:cd05959 160 THADDP--AFWL-YSSGSTGRPKGVVHLHADIYWTAELyarNVL--GIREDDVCFSAAKLFFAyglgNSLTFPLS----V 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 255 GTHVCLRWVRPKP--IYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMAdAGFAVTHL 332
Cdd:cd05959 231 GATTVLMPERPTPaaVFKRIRRYRPTVFFGVPTLYAAMLAAPNLPSRDLSSLRLCVSAGEALPAEVGERWK-ARFGLDIL 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 333 YGLTET-----YgpavvnewhgewddLAKGERSAKKARQGVRYAALEgLTVMDPETMQAtpADGEtIGEVMFRGNIVMKG 407
Cdd:cd05959 310 DGIGSTemlhiF--------------LSNRPGRVRYGTTGKPVPGYE-VELRDEDGGDV--ADGE-PGELYVRGPSSATM 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 408 YLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVP 487
Cdd:cd05959 372 YWNNRDKTRDTFQGEWTRTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDGLTKP 451
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 499217198 488 VAYVELKPGKTASEA---EIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLR 540
Cdd:cd05959 452 KAFVVLRPGYEDSEAleeELKEFVKDRLAPYKYPRWIVFvDELPKTATGKIQRFKLR 508
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
25-541 |
4.70e-59 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 204.99 E-value: 4.70e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLD 104
Cdd:PRK06155 27 LARQAERYPDRPLLVFGGTRWTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPINTALR 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 105 AAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVIDYDDPDYAADAPypkgerigALDYEDFVAGGDEDFAWSMP 184
Cdd:PRK06155 107 GPQLEHILRNSGARLLVVEAALLAALEAADPGDLPLPAVWLLDAPASVSVPA--------GWSTAPLPPLDAPAPAAAVQ 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 185 DDewDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLRWVR 264
Cdd:PRK06155 179 PG--DTAAILYTSGTTGPSKGVCCPHAQFYWWGRNSAEDLEIGADDVLYTTLPLFHTNALNAFFQALLAGATYVLEPRFS 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 265 PKPIYDAIADHG--VTHLCGAPVVMSVLINARDEDKrtfAQTVTFNTAAAPPPEAVLSGMADAGFAVTHLYGLTETYGPA 342
Cdd:PRK06155 257 ASGFWPAVRRHGatVTYLLGAMVSILLSQPARESDR---AHRVRVALGPGVPAALHAAFRERFGVDLLDGYGSTETNFVI 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 343 VVnewhgewddlakgerSAKKARQGVRYAALEGLT--VMDpETMQATPADgeTIGEVMFRGN---IVMKGYLKNRKASDE 417
Cdd:PRK06155 334 AV---------------THGSQRPGSMGRLAPGFEarVVD-EHDQELPDG--EPGELLLRADepfAFATGYFGMPEKTVE 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 418 AFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWG-EVPVAYVeLKPG 496
Cdd:PRK06155 396 AWRNLWFHTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSELGEdEVMAAVV-LRDG 474
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 499217198 497 KTASEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLRE 541
Cdd:PRK06155 475 TALEPVALVRHCEPRLAYFAVPRYVEFvAALPKTENGKVQKFVLRE 520
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
34-541 |
1.04e-57 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 198.67 E-value: 1.04e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 34 DHIAIIHGRQRISYRDFWRRSLKVASALQ-KRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTrldaaviafQL 112
Cdd:cd05941 1 DRIAIVDDGDSITYADLVARAARLANRLLaLGKDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNP---------SY 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 113 DHAETKVLIVDREFSGVVRQALDLakvtplvidyddpdyaadapypkgerigaldyedfvaggdedfawsmpddewdais 192
Cdd:cd05941 72 PLAELEYVITDSEPSLVLDPALIL-------------------------------------------------------- 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 193 lnYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAG-THVCLRWVRPKPIYDA 271
Cdd:cd05941 96 --YTSGTTGRPKGVVLTHANLAANVRALVDAWRWTEDDVLLHVLPLHHVHGLVNALLCPLFAGaSVEFLPKFDPKEVAIS 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 272 IADHGVTHLCGAPVVMSVLINARDedkRTFAQTVTFNTAA-----------APPPEAVLSGMADA-GFAVTHLYGLTETy 339
Cdd:cd05941 174 RLMPSITVFMGVPTIYTRLLQYYE---AHFTDPQFARAAAaerlrlmvsgsAALPVPTLEEWEAItGHTLLERYGMTEI- 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 340 GPAVVNEWHGEwddlakgersakkARQGVRYAALEG--LTVMDPETMQatPADGETIGEVMFRGNIVMKGYLKNRKASDE 417
Cdd:cd05941 250 GMALSNPLDGE-------------RRPGTVGMPLPGvqARIVDEETGE--PLPRGEVGEIQVRGPSVFKEYWNKPEATKE 314
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 418 AF-AGGWFHSGDLGVMHPDGYIQLKDRSK-DIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKP 495
Cdd:cd05941 315 EFtDDGWFKTGDLGVVDEDGYYWILGRSSvDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVVVLRA 394
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 499217198 496 GKTA-SEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLRE 541
Cdd:cd05941 395 GAAAlSLEELKEWAKQRLAPYKRPRRLILVdELPRNAMGKVNKKELRK 442
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
36-541 |
7.14e-57 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 198.20 E-value: 7.14e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 36 IAIIHGRQR-ISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDH 114
Cdd:PRK08276 2 AVIMAPSGEvVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDD 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 115 AETKVLIVDREFSGVVRQALDLAK--VTPLVIDYddpdyaadapypkGERIGALDYEDFVAGGDEDfawsMPDDEWDAIS 192
Cdd:PRK08276 82 SGAKVLIVSAALADTAAELAAELPagVPLLLVVA-------------GPVPGFRSYEEALAAQPDT----PIADETAGAD 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 193 LNYTSGTTGNPKGVVYHHRG----------AALMAYTNTIHAGmakhAVYLWTLPMFHC--NGWCfpwtLAVQAGTH--- 257
Cdd:PRK08276 145 MLYSSGTTGRPKGIKRPLPGldpdeapgmmLALLGFGMYGGPD----SVYLSPAPLYHTapLRFG----MSALALGGtvv 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 258 VCLRWvRPKPIYDAIADHGVTHLCGAPVvMSVLINARDEDKRT--------FAQTvtfntAAAPPPEAVLSGMAD-AGFA 328
Cdd:PRK08276 217 VMEKF-DAEEALALIERYRVTHSQLVPT-MFVRMLKLPEEVRArydvsslrVAIH-----AAAPCPVEVKRAMIDwWGPI 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 329 VTHLYGLTETYGPAVVNEwhGEWddLAK-GerSAKKARQGVryaalegLTVMDPetmqatpaDGE-----TIGEVMFRGN 402
Cdd:PRK08276 290 IHEYYASSEGGGVTVITS--EDW--LAHpG--SVGKAVLGE-------VRILDE--------DGNelppgEIGTVYFEMD 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 403 IVMKGYLKNRKASDEAFAG-GWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDD 481
Cdd:PRK08276 349 GYPFEYHNDPEKTAAARNPhGWVTVGDVGYLDEDGYLYLTDRKSDMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDE 428
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 499217198 482 KWGEVPVAYVELKPGKTASEA---EIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLRE 541
Cdd:PRK08276 429 EMGERVKAVVQPADGADAGDAlaaELIAWLRGRLAHYKCPRSIDFeDELPRTPTGKLYKRRLRD 492
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
25-533 |
2.60e-56 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 197.41 E-value: 2.60e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLD 104
Cdd:PRK07798 9 FEAVADAVPDRVALVCGDRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRYV 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 105 AAVIAFQLDHAETKVLIVDREFSGVVRQAL-DLAKVTPLVidyddpdyAADAPYPKGERIGALDYEDFVAGGDE--DFAW 181
Cdd:PRK07798 89 EDELRYLLDDSDAVALVYEREFAPRVAEVLpRLPKLRTLV--------VVEDGSGNDLLPGAVDYEDALAAGSPerDFGE 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 182 SMPDDewdaISLNYTSGTTGNPKGVVYHHR-------GAALMAYTNTIHA--GMAKHA------VYLWTLPMFHCNGWCF 246
Cdd:PRK07798 161 RSPDD----LYLLYTGGTTGMPKGVMWRQEdifrvllGGRDFATGEPIEDeeELAKRAaagpgmRRFPAPPLMHGAGQWA 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 247 PWTLAVQAGTHVCLRWVR--PKPIYDAIADHGVThlcgapvVMSV--------LINARDEDKRT-FAQTVTFNTAAAPPP 315
Cdd:PRK07798 237 AFAALFSGQTVVLLPDVRfdADEVWRTIEREKVN-------VITIvgdamarpLLDALEARGPYdLSSLFAIASGGALFS 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 316 EAVLSGMADA--GFAVTHLYGLTET--YGPAVVnewhgewddlAKGERSAKKARQGVRyaalEGLTVMDPETMQATPADG 391
Cdd:PRK07798 310 PSVKEALLELlpNVVLTDSIGSSETgfGGSGTV----------AKGAVHTGGPRFTIG----PRTVVLDEDGNPVEPGSG 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 392 EtIGEVMFRGNIVMkGYLKNRKASDEAF--AGG--WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKH 467
Cdd:PRK07798 376 E-IGWIARRGHIPL-GYYKDPEKTAETFptIDGvrYAIPGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEALKAH 453
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 499217198 468 PSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGK 533
Cdd:PRK07798 454 PDVADALVVGVPDERWGQEVVAVVQLREGARPDLAELRAHCRSSLAGYKVPRAIWFVdEVQRSPAGK 520
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
24-541 |
6.63e-56 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 195.68 E-value: 6.63e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 24 YLERAAKTYPDHIAIIHG--RQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNT 101
Cdd:PRK13391 2 YPGIHAQTTPDKPAVIMAstGEVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNS 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 102 RLDAAVIAFQLDHAETKVLIVDREFSGVVRQAL-DLAKVTPLVIDYDDpdyaadapypkGERIGALDYEDFVAGGDEDfa 180
Cdd:PRK13391 82 HLTPAEAAYIVDDSGARALITSAAKLDVARALLkQCPGVRHRLVLDGD-----------GELEGFVGYAEAVAGLPAT-- 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 181 wsmP-DDEWDAISLNYTSGTTGNPKGVVY---HHRGAALMAYTNTIHA--GMAKHAVYLWTLPMFHCNgwcfP--WTLAV 252
Cdd:PRK13391 149 ---PiADESLGTDMLYSSGTTGRPKGIKRplpEQPPDTPLPLTAFLQRlwGFRSDMVYLSPAPLYHSA----PqrAVMLV 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 253 QA--GTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTF----AQTVTFntAAAPPPEAVLSGMADAG 326
Cdd:PRK13391 222 IRlgGTVIVMEHFDAEQYLALIEEYGVTHTQLVPTMFSRMLKLPEEVRDKYdlssLEVAIH--AAAPCPPQVKEQMIDWW 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 327 FAVTH-LYGLTETYGPAVVN--EWhgewddlakgersakKARQGVRYAALEGLTVMDPETMQATPADgeTIGEVMFRGNI 403
Cdd:PRK13391 300 GPIIHeYYAATEGLGFTACDseEW---------------LAHPGTVGRAMFGDLHILDDDGAELPPG--EPGTIWFEGGR 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 404 VMKgYLKNRKASDEAFA--GGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDD 481
Cdd:PRK13391 363 PFE-YLNDPAKTAEARHpdGTWSTVGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGVPNE 441
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 499217198 482 KWGEVPVAYVELKPGKTASEA---EIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLRE 541
Cdd:PRK13391 442 DLGEEVKAVVQPVDGVDPGPAlaaELIAFCRQRLSRQKCPRSIDFeDELPRLPTGKLYKRLLRD 505
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
4-544 |
1.61e-53 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 190.63 E-value: 1.61e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 4 SYEQDLDRNAA-NHQPLTplTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPM 82
Cdd:PRK06710 10 SYPEEIPSTISyDIQPLH--KYVEQMASRYPEKKALHFLGKDITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQA 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 83 LEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLI-VDREFSGV--VRQALDLAKVT----------PLVIDYDDP 149
Cdd:PRK06710 88 VIGYYGTLLAGGIVVQTNPLYTERELEYQLHDSGAKVILcLDLVFPRVtnVQSATKIEHVIvtriadflpfPKNLLYPFV 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 150 DYAADAPYPKGERIGALDYEDFVAGgDEDFAWSMP-DDEWDAISLNYTSGTTGNPKGVVYHHRGAAlmayTNTIhagMAK 228
Cdd:PRK06710 168 QKKQSNLVVKVSESETIHLWNSVEK-EVNTGVEVPcDPENDLALLQYTGGTTGFPKGVMLTHKNLV----SNTL---MGV 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 229 HAVY---------LWTLPMFHCNGWCFPWTLAVQAG-THVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDK 298
Cdd:PRK06710 240 QWLYnckegeevvLGVLPFFHVYGMTAVMNLSIMQGyKMVLIPKFDMKMVFEAIKKHKVTLFPGAPTIYIALLNSPLLKE 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 299 RTFAQTVTFNTAAAPPPEAVLSGMAD-AGFAVTHLYGLTETygpAVVNEWHGEWDDLAKGErsakkarQGVRYAALEGLt 377
Cdd:PRK06710 320 YDISSIRACISGSAPLPVEVQEKFETvTGGKLVEGYGLTES---SPVTHSNFLWEKRVPGS-------IGVPWPDTEAM- 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 378 VMDPETMQATPAdGEtIGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISS 457
Cdd:PRK06710 389 IMSLETGEALPP-GE-IGEIVVKGPQIMKGYWNKPEETAAVLQDGWLHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYP 466
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 458 IEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQK 536
Cdd:PRK06710 467 REVEEVLYEHEKVQEVVTIGVPDPYRGETVKAFVVLKEGTECSEEELNQFARKYLAAYKVPKVYEFrDELPKTTVGKILR 546
|
....*...
gi 499217198 537 FRLREMAK 544
Cdd:PRK06710 547 RVLIEEEK 554
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
29-541 |
2.36e-52 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 185.78 E-value: 2.36e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 29 AKTYPDHIAIIH--GRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAA 106
Cdd:PRK09088 5 ARLQPQRLAAVDlaLGRRWTYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSAS 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 107 VIAFQLDHAETKVLIVDREFSGVVRQALDLAkvtplvidyddpdyaadapypkgerigalDYEDFVAGGDEDFAWSMPDD 186
Cdd:PRK09088 85 ELDALLQDAEPRLLLGDDAVAAGRTDVEDLA-----------------------------AFIASADALEPADTPSIPPE 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 187 ewdAISLN-YTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAV-QAGTHVCLRWVR 264
Cdd:PRK09088 136 ---RVSLIlFTSGTSGQPKGVMLSERNLQQTAHNFGVLGRVDAHSSFLCDAPMFHIIGLITSVRPVLaVGGSILVSNGFE 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 265 PKPIYDAIADH--GVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADAGFAVTHLYGLTET---Y 339
Cdd:PRK09088 213 PKRTLGRLGDPalGITHYFCVPQMAQAFRAQPGFDAAALRHLTALFTGGAPHAAEDILGWLDDGIPMVDGFGMSEAgtvF 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 340 GPAVvnewhgEWDDLAKGERSAKKARQGVryaaleGLTVMDPETMQATPadGETiGEVMFRGNIVMKGYLKNRKASDEAF 419
Cdd:PRK09088 293 GMSV------DCDVIRAKAGAAGIPTPTV------QTRVVDDQGNDCPA--GVP-GELLLRGPNLSPGYWRRPQATARAF 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 420 AG-GWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKT 498
Cdd:PRK09088 358 TGdGWFRTGDIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPADGAP 437
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 499217198 499 ASEAEIIEHCRALLARFKVPKAV-IFAEIPKTSTGKIQKFRLRE 541
Cdd:PRK09088 438 LDLERIRSHLSTRLAKYKVPKHLrLVDALPRTASGKLQKARLRD 481
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
37-541 |
2.48e-52 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 186.06 E-value: 2.48e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 37 AIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAE 116
Cdd:PRK12406 4 TIISGDRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSG 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 117 TKVLIVDREFSGVVRQALD-----LAKVTPLVIDYDDPDYAADAPYpkgeRIGALDYEDFVAGGDedfAWSMPDDEWDAi 191
Cdd:PRK12406 84 ARVLIAHADLLHGLASALPagvtvLSVPTPPEIAAAYRISPALLTP----PAGAIDWEGWLAQQE---PYDGPPVPQPQ- 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 192 SLNYTSGTTGNPKGVvyhHRGA-----ALMAYTNTIHA-GMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLRWVRP 265
Cdd:PRK12406 156 SMIYTSGTTGHPKGV---RRAAptpeqAAAAEQMRALIyGLKPGIRALLTGPLYHSAPNAYGLRAGRLGGVLVLQPRFDP 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 266 KPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTF-AQTVTFNT-AAAPPPEAVLSGMADA-GFAVTHLYGLTETygPA 342
Cdd:PRK12406 233 EELLQLIERHRITHMHMVPTMFIRLLKLPEEVRAKYdVSSLRHVIhAAAPCPADVKRAMIEWwGPVIYEYYGSTES--GA 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 343 VVneWHGEWDDLAKgERSAKKARQGVRyaalegLTVMDPETMQATPADgetIGEVMFR--GN--IVMKGYLKNRKASDEa 418
Cdd:PRK12406 311 VT--FATSEDALSH-PGTVGKAAPGAE------LRFVDEDGRPLPQGE---IGEIYSRiaGNpdFTYHNKPEKRAEIDR- 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 419 faGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKT 498
Cdd:PRK12406 378 --GGFITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMAVVEPQPGAT 455
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 499217198 499 ASEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLRE 541
Cdd:PRK12406 456 LDEADIRAQLKARLAGYKVPKHIEImAELPREDSGKIFKRRLRD 499
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
25-534 |
2.92e-52 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 185.41 E-value: 2.92e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAII--HGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTR 102
Cdd:cd05923 7 LRRAASRAPDACAIAdpARGLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPR 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 103 LDAAVIAFqldhaetkvLIVDREFSGVVRQalDLAKVTPLVIDYDDPDYAADAPYPKGERIGALDYEDFVAGGDEDFAWS 182
Cdd:cd05923 87 LKAAELAE---------LIERGEMTAAVIA--VDAQVMDAIFQSGVRVLALSDLVGLGEPESAGPLIEDPPREPEQPAFV 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 183 MpddewdaislnYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGM--AKHAVYLWTLPMFHCNGW--CFPWTLAVQaGTHV 258
Cdd:cd05923 156 F-----------YTSGTTGLPKGAVIPQRAAESRVLFMSTQAGLrhGRHNVVLGLMPLYHVIGFfaVLVAALALD-GTYV 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 259 CLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFA--QTVTFntAAAPPPEAVLSGMADA--GFAVTHlYG 334
Cdd:cd05923 224 VVEEFDPADALKLIEQERVTSLFATPTHLDALAAAAEFAGLKLSslRHVTF--AGATMPDAVLERVNQHlpGEKVNI-YG 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 335 LTETYGpAVVNEwhgewdDLAKGERSAKKARQGVRYAALEGltvmDPETMQATPADGETIgeVMFRGNIVMKGYLKNRKA 414
Cdd:cd05923 301 TTEAMN-SLYMR------DARTGTEMRPGFFSEVRIVRIGG----SPDEALANGEEGELI--VAAAADAAFTGYLNQPEA 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 415 SDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELK 494
Cdd:cd05923 368 TAKKLQDGWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTACVVPR 447
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 499217198 495 PGkTASEAEIIEHCRAL-LARFKVPKAVIFA-EIPKTSTGKI 534
Cdd:cd05923 448 EG-TLSADELDQFCRASeLADFKRPRRYFFLdELPKNAMNKV 488
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
189-543 |
3.93e-52 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 181.52 E-value: 3.93e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 DAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLR----WVR 264
Cdd:cd05944 3 DVAAYFHTGGTTGTPKLAQHTHSNEVYNAWMLALNSLFDPDDVLLCGLPLFHVNGSVVTLLTPLASGAHVVLAgpagYRN 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 265 P---KPIYDAIADHGVTHLCGAPVVMSVLINA---RDEDKRTFAQTvtfntAAAPPPEAVLSGMADA-GFAVTHLYGLTE 337
Cdd:cd05944 83 PglfDNFWKLVERYRITSLSTVPTVYAALLQVpvnADISSLRFAMS-----GAAPLPVELRARFEDAtGLPVVEGYGLTE 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 338 TYGPAVVNEWHGEWDDLAKGERsakkarqgVRYAALEgLTVMDPETMQATPADGETIGEVMFRGNIVMKGYLKNRKASDE 417
Cdd:cd05944 158 ATCLVAVNPPDGPKRPGSVGLR--------LPYARVR-IKVLDGVGRLLRDCAPDEVGEICVAGPGVFGGYLYTEGNKNA 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 418 AFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGK 497
Cdd:cd05944 229 FVADGWLNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGA 308
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 499217198 498 TASEAEIIEHCRALLA-RFKVPKAV-IFAEIPKTSTGKIQKFRLREMA 543
Cdd:cd05944 309 VVEEEELLAWARDHVPeRAAVPKHIeVLEELPVTAVGKVFKPALRADA 356
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
33-543 |
1.20e-51 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 184.95 E-value: 1.20e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 33 PDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQL 112
Cdd:PRK06164 24 PDAVALIDEDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIAVNTRYRSHEVAHIL 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 113 DHAETKVLIVDREFSGV----VRQALDLAKVTPL-VIDYDDPDYAADAPYPKGERIGALDYEDF--VAGGDEDfawsmPD 185
Cdd:PRK06164 104 GRGRARWLVVWPGFKGIdfaaILAAVPPDALPPLrAIAVVDDAADATPAPAPGARVQLFALPDPapPAAAGER-----AA 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 186 DEWDAISLNYTSGTTGNPKGVVyhHRGAALMAYTNTIHA--GMAKHAVYLWTLPMfhCNGWCFPWTLAVQAG--THVCLr 261
Cdd:PRK06164 179 DPDAGALLFTTSGTTSGPKLVL--HRQATLLRHARAIARayGYDPGAVLLAALPF--CGVFGFSTLLGALAGgaPLVCE- 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 262 wvrpkPIYDA------IADHGVTHLCGAPVVMSVLINARDEDkRTFAQTVTFNTAA-APPPEAVLSGMADAGFAVTHLYG 334
Cdd:PRK06164 254 -----PVFDAartaraLRRHRVTHTFGNDEMLRRILDTAGER-ADFPSARLFGFASfAPALGELAALARARGVPLTGLYG 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 335 LTETYgpAVVNEWHGEWDDLAKGERSAKKARQGVRyaalegLTVMDPETMQATPaDGEtIGEVMFRGNIVMKGYLKNRKA 414
Cdd:PRK06164 328 SSEVQ--ALVALQPATDPVSVRIEGGGRPASPEAR------VRARDPQDGALLP-DGE-SGEIEIRAPSLMRGYLDNPDA 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 415 SDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVV-ARHDDKwgEVPVAYVE 492
Cdd:PRK06164 398 TARALtDDGYFRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQVVgATRDGK--TVPVAFVI 475
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 499217198 493 LKPGKTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTG---KIQKFRLREMA 543
Cdd:PRK06164 476 PTDGASPDEAGLMAACREALAGFKVPARVQVVEaFPVTESAngaKIQKHRLREMA 530
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
16-544 |
1.22e-51 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 184.58 E-value: 1.22e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 16 HQPLTplTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFG------V 89
Cdd:COG1021 24 GETLG--DLLRRRAERHPDRIAVVDGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFAlfragaI 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 90 PMtkAVLHSLNtrldAAVIAFQLDHAETKVLIVDREFSGVVRQAL--DLAKVTP---LVIDYddpdyaadapypkGErig 164
Cdd:COG1021 102 PV--FALPAHR----RAEISHFAEQSEAVAYIIPDRHRGFDYRALarELQAEVPslrHVLVV-------------GD--- 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 165 ALDYEDFVAGGDEDFAWSMPD-DEWDAISLNYTSGTTGNPKGV-------VYHHRGAALMAytntihaGMAKHAVYLWTL 236
Cdd:COG1021 160 AGEFTSLDALLAAPADLSEPRpDPDDVAFFQLSGGTTGLPKLIprthddyLYSVRASAEIC-------GLDADTVYLAAL 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 237 PMFHCNGWCFPWTLAV-QAG-THVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRT-------------F 301
Cdd:COG1021 233 PAAHNFPLSSPGVLGVlYAGgTVVLAPDPSPDTAFPLIERERVTVTALVPPLALLWLDAAERSRYDlsslrvlqvggakL 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 302 AQTVtfntAAAPPPEA------VLsGMADagfavthlyGLtetygpavVNewhgewddlakgersakkarqgvrYAALEg 375
Cdd:COG1021 313 SPEL----ARRVRPALgctlqqVF-GMAE---------GL--------VN------------------------YTRLD- 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 376 ltvmDPE----TMQATP-----------------ADGETiGEVMFRGNIVMKGYLKNRKASDEAF-AGGWFHSGDLGVMH 433
Cdd:COG1021 346 ----DPEevilTTQGRPispddevrivdedgnpvPPGEV-GELLTRGPYTIRGYYRAPEHNARAFtPDGFYRTGDLVRRT 420
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 434 PDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKpGKTASEAEIIEHCRAL-L 512
Cdd:COG1021 421 PDGYLVVEGRAKDQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYLGERSCAFVVPR-GEPLTLAELRRFLRERgL 499
|
570 580 590
....*....|....*....|....*....|...
gi 499217198 513 ARFKVPKAVIF-AEIPKTSTGKIQKFRLREMAK 544
Cdd:COG1021 500 AAFKLPDRLEFvDALPLTAVGKIDKKALRAALA 532
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
189-536 |
5.74e-51 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 177.69 E-value: 5.74e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 DAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVClrwvrPKPI 268
Cdd:cd17638 1 DVSDIMFTSGTTGRSKGVMCAHRQTLRAAAAWADCADLTEDDRYLIINPFFHTFGYKAGIVACLLTGATVV-----PVAV 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 269 YD------AIADHGVTHLCGAPVVMSVLINARDEDKRTFAQ-TVTFNTAAAPPPEAVLSGMADAGF-AVTHLYGLTEtyg 340
Cdd:cd17638 76 FDvdaileAIERERITVLPGPPTLFQSLLDHPGRKKFDLSSlRAAVTGAATVPVELVRRMRSELGFeTVLTAYGLTE--- 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 341 pAVVNEWHGEWDDLAKGERSAKKARQGVRyaalegLTVMDPetmqatpadgetiGEVMFRGNIVMKGYLKNRKASDEAF- 419
Cdd:cd17638 153 -AGVATMCRPGDDAETVATTCGRACPGFE------VRIADD-------------GEVLVRGYNVMQGYLDDPEATAEAId 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 420 AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTA 499
Cdd:cd17638 213 ADGWLHTGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVARPGVTL 292
|
330 340 350
....*....|....*....|....*....|....*...
gi 499217198 500 SEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQK 536
Cdd:cd17638 293 TEEDVIAWCRERLANYKVPRFVRFLdELPRNASGKVMK 330
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
41-541 |
7.32e-50 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 180.75 E-value: 7.32e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 41 GRQRISYRDFWRRSLKVASALQKR-GIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKV 119
Cdd:PRK05620 35 EQEQTTFAAIGARAAALAHALHDElGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIVHIINHAEDEV 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 120 LIVDREFSGvvrQALDLAKVTPLV--IDYDDPDYAADAPYPKGERIGALDYEDFVAGGDEDFAWsmPD-DEWDAISLNYT 196
Cdd:PRK05620 115 IVADPRLAE---QLGEILKECPCVraVVFIGPSDADSAAAHMPEGIKVYSYEALLDGRSTVYDW--PElDETTAAAICYS 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 197 SGTTGNPKGVVYHHRGAAL--MAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTlAVQAGTHVCL--RWVRPKPIYDAI 272
Cdd:PRK05620 190 TGTTGAPKGVVYSHRSLYLqsLSLRTTDSLAVTHGESFLCCVPIYHVLSWGVPLA-AFMSGTPLVFpgPDLSAPTLAKII 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 273 ADH--GVTHlcGAPVV-MSVLIN-ARDEDKRTFAQTVtFNTAAAPPPEAVLSGMADAGFAVTHLYGLTET-------YGP 341
Cdd:PRK05620 269 ATAmpRVAH--GVPTLwIQLMVHyLKNPPERMSLQEI-YVGGSAVPPILIKAWEERYGVDVVHVWGMTETspvgtvaRPP 345
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 342 AVVNewhGEwddlakgERSAKKARQGVRYAALEGLTVMDPETMQATPADGetiGEVMFRGNIVMKGYLKN---------- 411
Cdd:PRK05620 346 SGVS---GE-------ARWAYRVSQGRFPASLEYRIVNDGQVMESTDRNE---GEIQVRGNWVTASYYHSpteegggaas 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 412 -------RKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWG 484
Cdd:PRK05620 413 tfrgedvEDANDRFTADGWLRTGDVGSVTRDGFLTIHDRARDVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDDKWG 492
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 499217198 485 EVPVAYVELKPG--KTASEAE-IIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLRE 541
Cdd:PRK05620 493 ERPLAVTVLAPGiePTRETAErLRDQLRDRLPNWMLPEYWTFVdEIDKTSVGKFDKKDLRQ 553
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
11-539 |
5.09e-48 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 173.67 E-value: 5.09e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 11 RNAANHQPLTPLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVP 90
Cdd:cd05920 7 RAAGYWQDEPLGDLLARSAARHPDRIAVVDGDRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALL 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 91 MTKAV-LHSLNTRLDAAVIAFqLDHAETKVLIVDREFSGVVRQAL--DLAKVTPLVidyddpdyaadapypkgerigALd 167
Cdd:cd05920 87 RLGAVpVLALPSHRRSELSAF-CAHAEAVAYIVPDRHAGFDHRALarELAESIPEV---------------------AL- 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 168 yedfvaggdedfawsmpddewdaisLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFP 247
Cdd:cd05920 144 -------------------------FLLSGGTTGTPKLIPRTHNDYAYNVRASAEVCGLDQDTVYLAVLPAAHNFPLACP 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 248 WTLAVQA--GTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAP-PPEAVLSGMAD 324
Cdd:cd05920 199 GVLGTLLagGRVVLAPDPSPDAAFPLIEREGVTVTALVPALVSLWLDAAASRRADLSSLRLLQVGGARlSPALARRVPPV 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 325 AGFAVTHLYGLTEtygpAVVNewHGEWDDlaKGERSAkkARQGVRYAALEGLTVMDPEtmqATP-ADGETiGEVMFRGNI 403
Cdd:cd05920 279 LGCTLQQVFGMAE----GLLN--YTRLDD--PDEVII--HTQGRPMSPDDEIRVVDEE---GNPvPPGEE-GELLTRGPY 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 404 VMKGYLKNRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDK 482
Cdd:cd05920 345 TIRGYYRAPEHNARAFtPDGFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAMPDEL 424
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 499217198 483 WGEVPVAYVELKPGKTaSEAEIIEHCRAL-LARFKVPKAVIFAE-IPKTSTGKIQKFRL 539
Cdd:cd05920 425 LGERSCAFVVLRDPPP-SAAQLRRFLRERgLAAYKLPDRIEFVDsLPLTAVGKIDKKAL 482
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
195-532 |
7.53e-48 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 169.40 E-value: 7.53e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 195 YTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLRWVRPKPIYDAIAD 274
Cdd:cd17636 7 YTAAFSGRPNGALLSHQALLAQALVLAVLQAIDEGTVFLNSGPLFHIGTLMFTLATFHAGGTNVFVRRVDAEEVLELIEA 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 275 HGVTH-LCGAPVVMSVL-INA-RDEDKRTFaqtvtFNTAAAPPPEAVLsgMADAGFAVTHL--YGLTETYGPAVVNeWHG 349
Cdd:cd17636 87 ERCTHaFLLPPTIDQIVeLNAdGLYDLSSL-----RSSPAAPEWNDMA--TVDTSPWGRKPggYGQTEVMGLATFA-ALG 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 350 EwddlaKGERSAKKARQGVRyaalegLTVMDPETMQAtpADGETiGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDL 429
Cdd:cd17636 159 G-----GAIGGAGRPSPLVQ------VRILDEDGREV--PDGEV-GEIVARGPTVMAGYWNRPEVNARRTRGGWHHTNDL 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 430 GVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCR 509
Cdd:cd17636 225 GRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKAIVVLKPGASVTEAELIEHCR 304
|
330 340
....*....|....*....|....
gi 499217198 510 ALLARFKVPKAVIFAE-IPKTSTG 532
Cdd:cd17636 305 ARIASYKKPKSVEFADaLPRTAGG 328
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
28-542 |
1.17e-47 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 173.96 E-value: 1.17e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 28 AAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAV 107
Cdd:PRK07788 58 AARRAPDRAALIDERGTLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLNTGFSGPQ 137
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 108 IAFQLDHAETKVLIVDREFSGVVRQAL-DLAKVTPLVIDYDDPDYAADapypkgeriGALDYEDFVAGGDEDfAWSMPDD 186
Cdd:PRK07788 138 LAEVAAREGVKALVYDDEFTDLLSALPpDLGRLRAWGGNPDDDEPSGS---------TDETLDDLIAGSSTA-PLPKPPK 207
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 187 EWDAISLnyTSGTTGNPKGVVyhhRGAALmayTNTIHAGMAKH------AVYLWTLPMFHCNGWCfPWTLAVQAGTHVCL 260
Cdd:PRK07788 208 PGGIVIL--TSGTTGTPKGAP---RPEPS---PLAPLAGLLSRvpfragETTLLPAPMFHATGWA-HLTLAMALGSTVVL 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 261 RwvR---PKPIYDAIADHGVTHLCGAPVVMSVLINARDE---DKRTFAQTVTFNTAAAPPPEAVLSGMADAGFAVTHLYG 334
Cdd:PRK07788 279 R--RrfdPEATLEDIAKHKATALVVVPVMLSRILDLGPEvlaKYDTSSLKIIFVSGSALSPELATRALEAFGPVLYNLYG 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 335 LTETYGPAVVNEwhgewDDLAKGERSAKKARQGVRYAALegltvmDPETMQATPadgETIGEVMFRGNIVMKGYLKNRka 414
Cdd:PRK07788 357 STEVAFATIATP-----EDLAEAPGTVGRPPKGVTVKIL------DENGNEVPR---GVVGRIFVGNGFPFEGYTDGR-- 420
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 415 sDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELK 494
Cdd:PRK07788 421 -DKQIIDGLLSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFGQRLRAFVVKA 499
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 499217198 495 PGKTASEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLREM 542
Cdd:PRK07788 500 PGAALDEDAIKDYVRDNLARYKVPRDVVFLdELPRNPTGKVLKRELREM 548
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
37-540 |
1.54e-46 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 168.81 E-value: 1.54e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 37 AIIHGRQRISYRDFWRRSLKVASAL-QKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHA 115
Cdd:cd05958 3 CLRSPEREWTYRDLLALANRIANVLvGELGIVPGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILDKA 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 116 E-TKVLIVDREfsgvvrQALDlakvtplvidyddpdyaadapypkgerigaldyedfvaggdedfawsmpddewDAISLN 194
Cdd:cd05958 83 RiTVALCAHAL------TASD-----------------------------------------------------DICILA 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 195 YTSGTTGNPKGVVYHHRGAALMAYTNTIHA-GMAKHAVYLWTLPMFHCNGW----CFPWtlAVQAGThVCLRWVRPKPIY 269
Cdd:cd05958 104 FTSGTTGAPKATMHFHRDPLASADRYAVNVlRLREDDRFVGSPPLAFTFGLggvlLFPF--GVGASG-VLLEEATPDLLL 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 270 DAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADA-GFAVTHLYGLTETYGPAVVNewh 348
Cdd:cd05958 181 SAIARYKPTVLFTAPTAYRAMLAHPDAAGPDLSSLRKCVSAGEALPAALHRAWKEAtGIPIIDGIGSTEMFHIFISA--- 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 349 gewddlAKGErsakkARQGVRYAALEGLT--VMDPEtmqATPADGETIGEVMFRGNIVMKGYLKNRKASdeAFAGGWFHS 426
Cdd:cd05958 258 ------RPGD-----ARPGATGKPVPGYEakVVDDE---GNPVPDGTIGRLAVRGPTGCRYLADKRQRT--YVQGGWNIT 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 427 GDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEA---E 503
Cdd:cd05958 322 GDTYSRDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGPVlarE 401
|
490 500 510
....*....|....*....|....*....|....*...
gi 499217198 504 IIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLR 540
Cdd:cd05958 402 LQDHAKAHIAPYKYPRAIEFvTELPRTATGKLQRFALR 439
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
37-540 |
1.65e-46 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 168.41 E-value: 1.65e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 37 AIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAE 116
Cdd:cd05919 3 AFYAADRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCE 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 117 TKVLIVDREfsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvaggdedfawsmpddewDAISLNYT 196
Cdd:cd05919 83 ARLVVTSAD---------------------------------------------------------------DIAYLLYS 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 197 SGTTGNPKGVVYHHRGAALMAYTNTIHA-GMAKHAVYLWTLPMFHC----NGWCFPWtlAVQAGTHVCLRWVRPKPIYDA 271
Cdd:cd05919 100 SGTTGPPKGVMHAHRDPLLFADAMAREAlGLTPGDRVFSSAKMFFGyglgNSLWFPL--AVGASAVLNPGWPTAERVLAT 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 272 IADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADA-GFAVTHLYGLTETYGPAVVNEwHGE 350
Cdd:cd05919 178 LARFRPTVLYGVPTFYANLLDSCAGSPDALRSLRLCVSAGEALPRGLGERWMEHfGGPILDGIGATEVGHIFLSNR-PGA 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 351 WddlakgersakkaRQGVRYAALEG--LTVMDPETMQATPAdgeTIGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGD 428
Cdd:cd05919 257 W-------------RLGSTGRPVPGyeIRLVDEEGHTIPPG---EEGDLLVRGPSAAVGYWNNPEKSRATFNGGWYRTGD 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 429 LGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEA---EII 505
Cdd:cd05919 321 KFCRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQESlarDIH 400
|
490 500 510
....*....|....*....|....*....|....*.
gi 499217198 506 EHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLR 540
Cdd:cd05919 401 RHLLERLSAHKVPRRIAFVdELPRTATGKLQRFKLR 436
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
189-534 |
1.69e-46 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 165.52 E-value: 1.69e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 DAISLNYTSGTTGNPKGVVYHHRGAaLMAYTNTIHA-GMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLRWVRPKP 267
Cdd:cd17637 1 DPFVIIHTAAVAGRPRGAVLSHGNL-IAANLQLIHAmGLTEADVYLNMLPLFHIAGLNLALATFHAGGANVVMEKFDPAE 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 268 IYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADAGFAVthLYGLTETYGPAVVNEW 347
Cdd:cd17637 80 ALELIEEEKVTLMGSFPPILSNLLDAAEKSGVDLSSLRHVLGLDAPETIQRFEETTGATFWS--LYGQTETSGLVTLSPY 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 348 HgewddlakgERSAKKARQGvryaALEGLTVMDpETMQATPAdGETiGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSG 427
Cdd:cd17637 158 R---------ERPGSAGRPG----PLVRVRIVD-DNDRPVPA-GET-GEIVVRGPLVFQGYWNLPELTAYTFRNGWHHTG 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 428 DLGVMHPDGYIQLKDRS--KDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEII 505
Cdd:cd17637 222 DLGRFDEDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEGIKAVCVLKPGATLTADELI 301
|
330 340 350
....*....|....*....|....*....|
gi 499217198 506 EHCRALLARFKVPKAVIFAE-IPKTSTGKI 534
Cdd:cd17637 302 EFVGSRIARYKKPRYVVFVEaLPKTADGSI 331
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
24-541 |
2.34e-46 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 170.31 E-value: 2.34e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 24 YLERAAKTYPDHIAII--HGrQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNT 101
Cdd:PRK06087 28 YWQQTARAMPDKIAVVdnHG-ASYTYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLLP 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 102 RLDAAVIAFQLDHAETKVLIVDREFSGV--VRQALDLAKVTPLVidyddpdYAADAPYPKGERIGALDYEDFVAGGdEDF 179
Cdd:PRK06087 107 SWREAELVWVLNKCQAKMFFAPTLFKQTrpVDLILPLQNQLPQL-------QQIVGVDKLAPATSSLSLSQIIADY-EPL 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 180 AWSMPDDEWDAISLNYTSGTTGNPKGVVYHHRG--AALMAYTNTIHagMAKHAVYLWTLPMFHCNGWCFPWTLAVQAG-T 256
Cdd:PRK06087 179 TTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNilASERAYCARLN--LTWQDVFMMPAPLGHATGFLHGVTAPFLIGaR 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 257 HVCLRWVRPKPIYDAIADHGVTHLCGA-PVVMSVLiNARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADAGFAVTHLYGL 335
Cdd:PRK06087 257 SVLLDIFTPDACLALLEQQRCTCMLGAtPFIYDLL-NLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVYGS 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 336 TETYGPAVVN-----EWHGEWDdlakgersakkarqGVRYAALEgLTVMDpETMQATPADGEtiGEVMFRGNIVMKGYLK 410
Cdd:PRK06087 336 TESSPHAVVNlddplSRFMHTD--------------GYAAAGVE-IKVVD-EARKTLPPGCE--GEEASRGPNVFMGYLD 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 411 NRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVA 489
Cdd:PRK06087 398 EPELTARALdEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCA 477
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 499217198 490 YVELK-PGKTASEAEIIEH-CRALLARFKVP-KAVIFAEIPKTSTGKIQKFRLRE 541
Cdd:PRK06087 478 YVVLKaPHHSLTLEEVVAFfSRKRVAKYKYPeHIVVIDKLPRTASGKIQKFLLRK 532
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
40-540 |
3.96e-46 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 167.61 E-value: 3.96e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 40 HGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKV 119
Cdd:cd05971 2 GTPEKVTFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASA 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 120 LIVDrefsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvaggdedfawsMPDDEwdAIsLNYTSGT 199
Cdd:cd05971 82 LVTD-----------------------------------------------------------GSDDP--AL-IIYTSGT 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 200 TGNPKGVVYHHRgaALMAytntiHAGmakhaVYLWTLPMFHCNGWCFpWTLAVQA---------------GTHVCLRWVR 264
Cdd:cd05971 100 TGPPKGALHAHR--VLLG-----HLP-----GVQFPFNLFPRDGDLY-WTPADWAwigglldvllpslyfGVPVLAHRMT 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 265 ---PKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADA-GFAVTHLYGLTETyg 340
Cdd:cd05971 167 kfdPKAALDLMSRYGVTTAFLPPTALKMMRQQGEQLKHAQVKLRAIATGGESLGEELLGWAREQfGVEVNEFYGQTEC-- 244
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 341 pavvNEWHGEWDDLAkgerSAKKARQGvryAALEGLTV--MDPETMQaTPADGETIGEVMFRGNIVMKGYLKNRKASDEA 418
Cdd:cd05971 245 ----NLVIGNCSALF----PIKPGSMG---KPIPGHRVaiVDDNGTP-LPPGEVGEIAVELPDPVAFLGYWNNPSATEKK 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 419 FAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKT 498
Cdd:cd05971 313 MAGDWLLTGDLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLNPGET 392
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 499217198 499 ASEA---EIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLR 540
Cdd:cd05971 393 PSDAlarEIQELVKTRLAAHEYPREIEFvNELPRTATGKIRRRELR 438
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
45-539 |
4.60e-46 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 167.77 E-value: 4.60e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 45 ISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDr 124
Cdd:cd05907 6 ITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKALFVE- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 125 efsgvvrQALDLAKVTplvidyddpdyaadapypkgerigaldyedfvaggdedfawsmpddewdaislnYTSGTTGNPK 204
Cdd:cd05907 85 -------DPDDLATII------------------------------------------------------YTSGTTGRPK 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 205 GVVYHHRG--AALMAYTNTIHAGmaKHAVYLWTLPMFHCngwcfpwtLAVQAGTHVCLR-------WVRPKPIYDAIADH 275
Cdd:cd05907 104 GVMLSHRNilSNALALAERLPAT--EGDRHLSFLPLAHV--------FERRAGLYVPLLagariyfASSAETLLDDLSEV 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 276 GVTHLCGAP-----VVMSVLINARDEDKRT-FAQTVTFN-----TAAAPPPEAVLSGMADAGFAVTHLYGLTETYGPAVV 344
Cdd:cd05907 174 RPTVFLAVPrvwekVYAAIKVKAVPGLKRKlFDLAVGGRlrfaaSGGAPLPAELLHFFRALGIPVYEGYGLTETSAVVTL 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 345 NEWHGEwddlakgersakkarqgvrYAALEGlTVMDPETMQATPAdgetiGEVMFRGNIVMKGYLKNRKASDEAF-AGGW 423
Cdd:cd05907 254 NPPGDN-------------------RIGTVG-KPLPGVEVRIADD-----GEILVRGPNVMLGYYKNPEATAEALdADGW 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 424 FHSGDLGVMHPDGYIQLKDRSKDIII-SGGENISSIEVEDALYKHPSVASCGVVARHDDKwgevPVAYVELKPGKTASEA 502
Cdd:cd05907 309 LHTGDLGEIDEDGFLHITGRKKDLIItSGGKNISPEPIENALKASPLISQAVVIGDGRPF----LVALIVPDPEALEAWA 384
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 499217198 503 E---IIEHCRALLARFKVPKAVIFAEIPKTSTG-----KIQKFRL 539
Cdd:cd05907 385 EehgIAYTDVAELAANPAVRAEIEAAVEAANARlsryeQIKKFLL 429
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
29-541 |
4.78e-46 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 169.77 E-value: 4.78e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 29 AKTYPDHIAIIHGR--QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAA 106
Cdd:PLN02330 38 AELYADKVAFVEAVtgKAVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGVFSGANPTALES 117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 107 VIAFQLDHAETKVLIVDREFSGVVRqALDLakvtPLVIDYDDPDYaadapypkgeriGALDYEDFVAGGDEdfAWSMPDD 186
Cdd:PLN02330 118 EIKKQAEAAGAKLIVTNDTNYGKVK-GLGL----PVIVLGEEKIE------------GAVNWKELLEAADR--AGDTSDN 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 187 E----WDAISLNYTSGTTGNPKGVVYHHRG--AALMAYTNTIHAGMAKHAVYLWTLPMFHC---NGWCFPwTLAvQAGTH 257
Cdd:PLN02330 179 EeilqTDLCALPFSSGTTGISKGVMLTHRNlvANLCSSLFSVGPEMIGQVVTLGLIPFFHIygiTGICCA-TLR-NKGKV 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 258 VCLRWVRPKPIYDAIADHGVTHlcgAPVVMSVLIN-----ARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADA--GFAVT 330
Cdd:PLN02330 257 VVMSRFELRTFLNALITQEVSF---APIVPPIILNlvknpIVEEFDLSKLKLQAIMTAAAPLAPELLTAFEAKfpGVQVQ 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 331 HLYGLTETygpAVVNEWHGewdDLAKGERSAKKARQGVRYAALEgLTVMDPETMQATPADgeTIGEVMFRGNIVMKGYLK 410
Cdd:PLN02330 334 EAYGLTEH---SCITLTHG---DPEKGHGIAKKNSVGFILPNLE-VKFIDPDTGRSLPKN--TPGELCVRSQCVMQGYYN 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 411 NRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVA 489
Cdd:PLN02330 405 NKEETDRTIdEDGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLPDEEAGEIPAA 484
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 499217198 490 YVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKIQKFRLRE 541
Cdd:PLN02330 485 CVVINPKAKESEEDILNFVAANVAHYKKVRVVQFVDsIPKSLSGKIMRRLLKE 537
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
19-541 |
6.94e-46 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 169.17 E-value: 6.94e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 19 LTPLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHS 98
Cdd:PRK13382 43 MGPTSGFAIAAQRCPDRPGLIDELGTLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGADILL 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 99 LNTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQALDlakvtplvidyddpdyaadaPYPKGERIGA---LDYEDFVAGG 175
Cdd:PRK13382 123 LNTSFAGPALAEVVTREGVDTVIYDEEFSATVDRALA--------------------DCPQATRIVAwtdEDHDLTVEVL 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 176 DEDFAWSMP---DDEWDAISLnyTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHcnGWCFPWTL-- 250
Cdd:PRK13382 183 IAAHAGQRPeptGRKGRVILL--TSGTTGTPKGARRSGPGGIGTLKAILDRTPWRAEEPTVIVAPMFH--AWGFSQLVla 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 251 AVQAGTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDK-RTFAQTVTFNTAAAP--PPEAVLSGMADAGF 327
Cdd:PRK13382 259 ASLACTIVTRRRFDPEATLDLIDRHRATGLAVVPVMFDRIMDLPAEVRnRYSGRSLRFAAASGSrmRPDVVIAFMDQFGD 338
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 328 AVTHLYGLTETYGPAVVNEwhgewDDLAKGERSAKKARQGVRyaalegLTVMDPEtMQATPaDGEtIGEVMFRGNIVMKG 407
Cdd:PRK13382 339 VIYNNYNATEAGMIATATP-----ADLRAAPDTAGRPAEGTE------IRILDQD-FREVP-TGE-VGTIFVRNDTQFDG 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 408 YlknRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVP 487
Cdd:PRK13382 405 Y---TSGSTKDFHDGFMASGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRL 481
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 499217198 488 VAYVELKPGKTASEAEIIEHCRALLARFKVPKA-VIFAEIPKTSTGKIQKFRLRE 541
Cdd:PRK13382 482 AAFVVLKPGASATPETLKQHVRDNLANYKVPRDiVVLDELPRGATGKILRRELQA 536
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
159-541 |
1.16e-45 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 167.17 E-value: 1.16e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 159 KGERIGALDYEDFVAGGDEdfawSMPDDEWDAISLNYTSGTTGNPKGVVYHH----------RGAALMAytntihaGMAK 228
Cdd:cd05929 100 GGALDGLEDYEAAEGGSPE----TPIEDEAAGWKMLYSGGTTGRPKGIKRGLpggppdndtlMAAALGF-------GPGA 168
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 229 HAVYLWTLPMFHCNGwcFPWTLAVQA--GTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDE--DKRTFAQT 304
Cdd:cd05929 169 DSVYLSPAPLYHAAP--FRWSMTALFmgGTLVLMEKFDPEEFLRLIERYRVTFAQFVPTMFVRLLKLPEAvrNAYDLSSL 246
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 305 VTFNTAAAPPPEAVLSGMADAGFAVTH-LYGLTETYGPAVVN--EW--HgewddlaKGerSAKKARQGvryaaleGLTVM 379
Cdd:cd05929 247 KRVIHAAAPCPPWVKEQWIDWGGPIIWeYYGGTEGQGLTIINgeEWltH-------PG--SVGRAVLG-------KVHIL 310
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 380 DpETMQATPADgeTIGEVMFRGN---IVMKGYLKNRKASDEafaGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENIS 456
Cdd:cd05929 311 D-EDGNEVPPG--EIGEVYFANGpgfEYTNDPEKTAAARNE---GGWSTLGDVGYLDEDGYLYLTDRRSDMIISGGVNIY 384
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 457 SIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEA---EIIEHCRALLARFKVPKAVIF-AEIPKTSTG 532
Cdd:cd05929 385 PQEIENALIAHPKVLDAAVVGVPDEELGQRVHAVVQPAPGADAGTAlaeELIAFLRDRLSRYKCPRSIEFvAELPRDDTG 464
|
....*....
gi 499217198 533 KIQKFRLRE 541
Cdd:cd05929 465 KLYRRLLRD 473
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
43-540 |
3.85e-45 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 166.40 E-value: 3.85e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 43 QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIV 122
Cdd:PRK08008 36 RRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESAWILQNSQASLLVT 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 123 DREFSGVVRQALDLAKVTP---LVIDYDDpdyaadapypkGERIGALDYEDFVAGGDEDFAWSMPDDEWDAISLNYTSGT 199
Cdd:PRK08008 116 SAQFYPMYRQIQQEDATPLrhiCLTRVAL-----------PADDGVSSFTQLKAQQPATLCYAPPLSTDDTAEILFTSGT 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 200 TGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAG-THVCLRWVRPKPIYDAIADH--G 276
Cdd:PRK08008 185 TSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYLTVMPAFHIDCQCTAAMAAFSAGaTFVLLEKYSARAFWGQVCKYraT 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 277 VTH--------LCGAPVV----------MSVLINARDEDKRTFAQTvtfntaaapppeavlsgmadagFAVTHL--YGLT 336
Cdd:PRK08008 265 ITEcipmmirtLMVQPPSandrqhclreVMFYLNLSDQEKDAFEER----------------------FGVRLLtsYGMT 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 337 ETYGPAVvnewhgewddlakGERSAKK------ARQGVRYAAleglTVMDPetmQATPADGETIGEVMFRG---NIVMKG 407
Cdd:PRK08008 323 ETIVGII-------------GDRPGDKrrwpsiGRPGFCYEA----EIRDD---HNRPLPAGEIGEICIKGvpgKTIFKE 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 408 YLKNRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEV 486
Cdd:PRK08008 383 YYLDPKATAKVLeADGWLHTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEA 462
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 499217198 487 PVAYVELKPGKTASEAEIIEHCRALLARFKVPKAV-IFAEIPKTSTGKIQKFRLR 540
Cdd:PRK08008 463 IKAFVVLNEGETLSEEEFFAFCEQNMAKFKVPSYLeIRKDLPRNCSGKIIKKNLK 517
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
27-541 |
1.28e-44 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 164.66 E-value: 1.28e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 27 RAAKTYPDHIAI-IHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDA 105
Cdd:PRK07514 10 RAAFADRDAPFIeTPDGLRYTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYTL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 106 AVIAFQLDHAETKVLIVD-REFSGV--VRQALDLAKVTPLvidyddpdyaadapypKGERIGALdyEDFVAGGDEDFAwS 182
Cdd:PRK07514 90 AELDYFIGDAEPALVVCDpANFAWLskIAAAAGAPHVETL----------------DADGTGSL--LEAAAAAPDDFE-T 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 183 MPDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGwcfpwtLAVqaGTHVCLR- 261
Cdd:PRK07514 151 VPRGADDLAAILYTSGTTGRSKGAMLSHGNLLSNALTLVDYWRFTPDDVLIHALPIFHTHG------LFV--ATNVALLa 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 262 -----WvRPKPIYDAIADHgvthLCGAPVVMSV------LINARDEDKRTFAQTVTFNTAAAP-PPEAVLSGMADAGFAV 329
Cdd:PRK07514 223 gasmiF-LPKFDPDAVLAL----MPRATVMMGVptfytrLLQEPRLTREAAAHMRLFISGSAPlLAETHREFQERTGHAI 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 330 THLYGLTETyGPAVVNEWHGEwddlakgeRsakkaRQGVRYAALEG--LTVMDPETMQATPADGetIGEVMFRGNIVMKG 407
Cdd:PRK07514 298 LERYGMTET-NMNTSNPYDGE--------R-----RAGTVGFPLPGvsLRVTDPETGAELPPGE--IGMIEVKGPNVFKG 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 408 YLKNRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVV-ARHDDkWGE 485
Cdd:PRK07514 362 YWRMPEKTAEEFrADGFFITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVESAVIgVPHPD-FGE 440
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 499217198 486 VPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLRE 541
Cdd:PRK07514 441 GVTAVVVPKPGAALDEAAILAALKGRLARFKQPKRVFFVdELPRNTMGKVQKNLLRE 497
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
25-476 |
4.15e-44 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 164.89 E-value: 4.15e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIH----GRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLN 100
Cdd:COG1022 17 LRRRAARFPDRVALREkedgIWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAVTVPIY 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 101 TRLDAAVIAFQLDHAETKVLIV-DREFSGVVRQALD-LAKVTPLVIDYDDPDYaadapypkgERIGALDYEDFVAGGDED 178
Cdd:COG1022 97 PTSSAEEVAYILNDSGAKVLFVeDQEQLDKLLEVRDeLPSLRHIVVLDPRGLR---------DDPRLLSLDELLALGREV 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 179 FAWSMPDDEWDAISLN------YTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHC--NGWCFpwtL 250
Cdd:COG1022 168 ADPAELEARRAAVKPDdlatiiYTSGTTGRPKGVMLTHRNLLSNARALLERLPLGPGDRTLSFLPLAHVfeRTVSY---Y 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 251 AVQAGTHVC-----------LRWVRPkpiydaiadhgvTHLCGAP-----VVMSVLINARDED----------------- 297
Cdd:COG1022 245 ALAAGATVAfaespdtlaedLREVKP------------TFMLAVPrvwekVYAGIQAKAEEAGglkrklfrwalavgrry 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 298 ----------------KRTFAQTVTFN--------------TAAAPPPEAVLSGMADAGFAVTHLYGLTETYGPAVVNew 347
Cdd:COG1022 313 ararlagkspslllrlKHALADKLVFSklrealggrlrfavSGGAALGPELARFFRALGIPVLEGYGLTETSPVITVN-- 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 348 hgewddlakgerSAKKARQGvryaaleglTVmdpetmqATPADGETI-----GEVMFRGNIVMKGYLKNRKASDEAF-AG 421
Cdd:COG1022 391 ------------RPGDNRIG---------TV-------GPPLPGVEVkiaedGEILVRGPNVMKGYYKNPEATAEAFdAD 442
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 499217198 422 GWFHSGDLGVMHPDGYIQLKDRSKDIII-SGGENISSIEVEDALYKHPSVASCGVV 476
Cdd:COG1022 443 GWLHTGDIGELDEDGFLRITGRKKDLIVtSGGKNVAPQPIENALKASPLIEQAVVV 498
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
46-540 |
2.16e-43 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 159.98 E-value: 2.16e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 46 SYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDRE 125
Cdd:cd05969 2 TFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITTEE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 126 FsgvvrqaldLAKVTPlvidyddpdyaadapypkgerigaldyedfvaggdEDFAWsmpddewdaisLNYTSGTTGNPKG 205
Cdd:cd05969 82 L---------YERTDP-----------------------------------EDPTL-----------LHYTSGTTGTPKG 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 206 VVYHHRGAALMAYTNTIHAGMAKHAVYL------WTLPMFHcnGWCFPW----TLAVQAGTHVCLRWvrpkpiYDAIADH 275
Cdd:cd05969 107 VLHVHDAMIFYYFTGKYVLDLHPDDIYWctadpgWVTGTVY--GIWAPWlngvTNVVYEGRFDAESW------YGIIERV 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 276 GVTHLCGAPVVMSVLINARDEDKRTFAQT-VTFNTAAAPP--PEAVLSGMADAGFAVTHLYGLTETYGPAVVNEwhgEWD 352
Cdd:cd05969 179 KVTVWYTAPTAIRMLMKEGDELARKYDLSsLRFIHSVGEPlnPEAIRWGMEVFGVPIHDTWWQTETGSIMIANY---PCM 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 353 DLAKGerSAKKARQGVRYAalegltVMDpETMQATPADgeTIGEVMFRGNI--VMKGYLKNRKASDEAFAGGWFHSGDLG 430
Cdd:cd05969 256 PIKPG--SMGKPLPGVKAA------VVD-ENGNELPPG--TKGILALKPGWpsMFRGIWNDEERYKNSFIDGWYLTGDLA 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 431 VMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEA---EIIEH 507
Cdd:cd05969 325 YRDEDGYFWFVGRADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDElkeEIINF 404
|
490 500 510
....*....|....*....|....*....|....
gi 499217198 508 CRALLARFKVPKAVIFAE-IPKTSTGKIQKFRLR 540
Cdd:cd05969 405 VRQKLGAHVAPREIEFVDnLPKTRSGKIMRRVLK 438
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
45-540 |
2.80e-43 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 159.61 E-value: 2.80e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 45 ISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDr 124
Cdd:cd05973 1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVTD- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 125 efsgvvrqALDLAKVtplvidyddpdyaadapypkgerigaldyedfvaggDEDFAWSMpddewdaislnYTSGTTGNPK 204
Cdd:cd05973 80 --------AANRHKL------------------------------------DSDPFVMM-----------FTSGTTGLPK 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 205 GVVYHHRgaALMAYtntiHAGMaKHAVYLWTLPMFHC---NGWCFPWTLAVQA----GTHVCLRW--VRPKPIYDAIADH 275
Cdd:cd05973 105 GVPVPLR--ALAAF----GAYL-RDAVDLRPEDSFWNaadPGWAYGLYYAITGplalGHPTILLEggFSVESTWRVIERL 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 276 GVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPP--PEAVLSGMADAGFAVTHLYGLTEtYGPAVVNEWHgewdd 353
Cdd:cd05973 178 GVTNLAGSPTAYRLLMAAGAEVPARPKGRLRRVSSAGEPltPEVIRWFDAALGVPIHDHYGQTE-LGMVLANHHA----- 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 354 lakgerSAKKARQGVRYAALEG--LTVMDPETMQATPAD-GETIGEVMFRGNIVMKGYlknRKASDEAFAGGWFHSGDLG 430
Cdd:cd05973 252 ------LEHPVHAGSAGRAMPGwrVAVLDDDGDELGPGEpGRLAIDIANSPLMWFRGY---QLPDTPAIDGGYYLTGDTV 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 431 VMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEA---EIIEH 507
Cdd:cd05973 323 EFDPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHEGTPAladELQLH 402
|
490 500 510
....*....|....*....|....*....|....
gi 499217198 508 CRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLR 540
Cdd:cd05973 403 VKKRLSAHAYPRTIHFvDELPKTPSGKIQRFLLR 436
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
14-546 |
8.21e-43 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 160.53 E-value: 8.21e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 14 ANHQPLTplTY-LERAAKtYPDHIAIIHGR--QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVP 90
Cdd:PLN02246 20 PNHLPLH--DYcFERLSE-FSDRPCLIDGAtgRVYTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGAS 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 91 MTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVIdyddpdyaadapypKGERIGALDYED 170
Cdd:PLN02246 97 RRGAVTTTANPFYTPAEIAKQAKASGAKLIITQSCYVDKLKGLAEDDGVTVVTI--------------DDPPEGCLHFSE 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 171 FVAGGDEDfawsMPDDEW---DAISLNYTSGTTGNPKGVVYHHRG-----AALMAYTNTiHAGMAKHAVYLWTLPMFHCn 242
Cdd:PLN02246 163 LTQADENE----LPEVEIspdDVVALPYSSGTTGLPKGVMLTHKGlvtsvAQQVDGENP-NLYFHSDDVILCVLPMFHI- 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 243 gWCFPWTL--AVQAGTHVClrwVRPK----PIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPP-- 314
Cdd:PLN02246 237 -YSLNSVLlcGLRVGAAIL---IMPKfeigALLELIQRHKVTIAPFVPPIVLAIAKSPVVEKYDLSSIRMVLSGAAPLgk 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 315 ----------PEAVLsGMAdagfavthlYGLTETyGPAvvnewhgewddLAKGERSAK---KARQG-----VRYAALEgl 376
Cdd:PLN02246 313 eledafraklPNAVL-GQG---------YGMTEA-GPV-----------LAMCLAFAKepfPVKSGscgtvVRNAELK-- 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 377 tVMDPETMQATPADgeTIGEVMFRGNIVMKGYLKNRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENI 455
Cdd:PLN02246 369 -IVDPETGASLPRN--QPGEICIRGPQIMKGYLNDPEATANTIdKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQV 445
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 456 SSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKI 534
Cdd:PLN02246 446 APAELEALLISHPSIADAAVVPMKDEVAGEVPVAFVVRSNGSEITEDEIKQFVAKQVVFYKRIHKVFFVDsIPKAPSGKI 525
|
570
....*....|..
gi 499217198 535 QKFRLRemAKLA 546
Cdd:PLN02246 526 LRKDLR--AKLA 535
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
34-542 |
8.63e-43 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 159.62 E-value: 8.63e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 34 DHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLD 113
Cdd:TIGR02262 20 GKTAFIDDISSLSYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLGAIRAGIVPVALNTLLTADDYAYMLE 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 114 HAETKVLIVDREFSGVVRQAL-DLAKVTPLVIDYddpdyaadapypkGERIGALDYEDFVAGGDEDF--AWSMPDDE--W 188
Cdd:TIGR02262 100 DSRARVVFVSGALLPVIKAALgKSPHLEHRVVVG-------------RPEAGEVQLAELLATESEQFkpAATQADDPafW 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 daislNYTSGTTGNPKGVVYHHRGAALMAYT---NTIhaGMAKHAVYLWTLPMFHC----NGWCFPWTLavqaGTHVCLR 261
Cdd:TIGR02262 167 -----LYSSGSTGMPKGVVHTHSNPYWTAELyarNTL--GIREDDVCFSAAKLFFAyglgNALTFPMSV----GATTVLM 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 262 WVRPKP--IYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAV-LSGMADAGFAVTHLYGLTET 338
Cdd:TIGR02262 236 GERPTPdaVFDRLRRHQPTIFYGVPTLYAAMLADPNLPSEDQVRLRLCTSAGEALPAEVgQRWQARFGVDIVDGIGSTEM 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 339 YGPAVVNEWHgewdDLAKGERSAKKARQGVRYAALEGLTVmdpetmqatpADGEtIGEVMFRGNIVMKGYLKNRKASDEA 418
Cdd:TIGR02262 316 LHIFLSNLPG----DVRYGTSGKPVPGYRLRLVGDGGQDV----------ADGE-PGELLISGPSSATMYWNNRAKSRDT 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 419 FAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKT 498
Cdd:TIGR02262 381 FQGEWTRSGDKYVRNDDGSYTYAGRTDDMLKVSGIYVSPFEIESALIQHPAVLEAAVVGVADEDGLIKPKAFVVLRPGQT 460
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 499217198 499 ASEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLREM 542
Cdd:TIGR02262 461 ALETELKEHVKDRLAPYKYPRWIVFvDDLPKTATGKIQRFKLREG 505
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
33-534 |
1.37e-42 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 158.07 E-value: 1.37e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 33 PDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQL 112
Cdd:cd05930 1 PDAVAVVDGDQSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERLAYIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 113 DHAETKVLIVDrefsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvaggDEDFAWSMpddewdais 192
Cdd:cd05930 81 EDSGAKLVLTD----------------------------------------------------PDDLAYVI--------- 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 193 lnYTSGTTGNPKGVVYHHRG-AALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLRWVR--PKPIY 269
Cdd:cd05930 100 --YTSGSTGKPKGVMVEHRGlVNLLLWMQEAYPLTPGDRVLQFTSFSFDVSVWEIFGALLAGATLVVLPEEVRkdPEALA 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 270 DAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFntAAAPPPEAVLSGMADAGFAVT--HLYGLTETYGPAVVneW 347
Cdd:cd05930 178 DLLAEEGITVLHLTPSLLRLLLQELELAALPSLRLVLV--GGEALPPDLVRRWRELLPGARlvNLYGPTEATVDATY--Y 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 348 HGEWDDLAKGERSAKKARQGVRYAalegltVMDPEtMQATPaDGETiGEVMFRGNIVMKGYLKNRKASDEAF------AG 421
Cdd:cd05930 254 RVPPDDEEDGRVPIGRPIPNTRVY------VLDEN-LRPVP-PGVP-GELYIGGAGLARGYLNRPELTAERFvpnpfgPG 324
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 422 GWFH-SGDLGVMHPDGYIQLKDRSKDII-ISG-----GEnissieVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELK 494
Cdd:cd05930 325 ERMYrTGDLVRWLPDGNLEFLGRIDDQVkIRGyrielGE------IEAALLAHPGVREAAVVAREDGDGEKRLVAYVVPD 398
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 499217198 495 PGKTASEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKI 534
Cdd:cd05930 399 EGGELDEEELRAHLAERLPDYMVPSAFVVlDALPLTPNGKV 439
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
39-537 |
8.48e-42 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 156.06 E-value: 8.48e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 39 IHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETK 118
Cdd:cd05914 2 YYGGEPLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAK 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 119 VLIVdrefsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvagGDEDfawsmpddewDAISLNYTSG 198
Cdd:cd05914 82 AIFV----------------------------------------------------SDED----------DVALINYTSG 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 199 TTGNPKGVVYHHRG-AALMAYTNTIhaGMAKHA-VYLWTLPMFHCNGWCFPWTLAVQAGTHVCLRWVRPKPIYDAIADHG 276
Cdd:cd05914 100 TTGNSKGVMLTYRNiVSNVDGVKEV--VLLGKGdKILSILPLHHIYPLTFTLLLPLLNGAHVVFLDKIPSAKIIALAFAQ 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 277 VTHLCGAPVVMSV-------LINARDEDKRTFAQTVTFN------------------------TAAAPPPEAVLSGMADA 325
Cdd:cd05914 178 VTPTLGVPVPLVIekifkmdIIPKLTLKKFKFKLAKKINnrkirklafkkvheafggnikefvIGGAKINPDVEEFLRTI 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 326 GFAVTHLYGLTETyGPAVV-NEWHGEWDDlakgerSAKKARQGVryaalegltvmdpETMQATPADGETIGEVMFRGNIV 404
Cdd:cd05914 258 GFPYTIGYGMTET-APIISySPPNRIRLG------SAGKVIDGV-------------EVRIDSPDPATGEGEIIVRGPNV 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 405 MKGYLKNRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISG-GENISSIEVEDALYKHPSVASCGVVARHDDk 482
Cdd:cd05914 318 MKGYYKNPEATAEAFdKDGWFHTGDLGKIDAEGYLYIRGRKKEMIVLSsGKNIYPEEIEAKINNMPFVLESLVVVQEKK- 396
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 499217198 483 wgEVPVAYV--------ELKPG---KTASEAEIIEHCRALLARFKVPKAVI-FAEIPKTSTGKIQKF 537
Cdd:cd05914 397 --LVALAYIdpdfldvkALKQRniiDAIKWEVRDKVNQKVPNYKKISKVKIvKEEFEKTPKGKIKRF 461
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
25-543 |
4.43e-41 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 156.11 E-value: 4.43e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTR-- 102
Cdd:PLN02860 13 LTRLATLRGNAVVTISGNRRRTGHEFVDGVLSLAAGLLRLGLRNGDVVAIAALNSDLYLEWLLAVACAGGIVAPLNYRws 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 103 LDAAVIAFQLdhAETKVLIVDrefSGVVRQALDLAKVT-PLV---IDYDDPDYAADAPYPKGERIGALDYEDFVAGgDED 178
Cdd:PLN02860 93 FEEAKSAMLL--VRPVMLVTD---ETCSSWYEELQNDRlPSLmwqVFLESPSSSVFIFLNSFLTTEMLKQRALGTT-ELD 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 179 FAWSmPDDewdAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHV 258
Cdd:PLN02860 167 YAWA-PDD---AVLICFTSGTTGRPKGVTISHSALIVQSLAKIAIVGYGEDDVYLHTAPLCHIGGLSSALAMLMVGACHV 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 259 CLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLIN-ARDEDKRTFAQTVT--FNTAAAPP----PEAVLSGMADAGFAVth 331
Cdd:PLN02860 243 LLPKFDAKAALQAIKQHNVTSMITVPAMMADLISlTRKSMTWKVFPSVRkiLNGGGSLSsrllPDAKKLFPNAKLFSA-- 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 332 lYGLTE-----TYGPavVNEWHGEWDDLAKGERSAKKARQGVRYaalEGLTVMDPE---TMQATPADGETIGEVMFRGNI 403
Cdd:PLN02860 321 -YGMTEacsslTFMT--LHDPTLESPKQTLQTVNQTKSSSVHQP---QGVCVGKPAphvELKIGLDESSRVGRILTRGPH 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 404 VMKGYLKNRKA-SDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDK 482
Cdd:PLN02860 395 VMLGYWGQNSEtASVLSNDGWLDTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVGVPDSR 474
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 499217198 483 WGEVPVAYVELKPG--------------KTASEAEIIEHCRAL-LARFKVPKAVIFAE--IPKTSTGKIQKFRLREMA 543
Cdd:PLN02860 475 LTEMVVACVRLRDGwiwsdnekenakknLTLSSETLRHHCREKnLSRFKIPKLFVQWRkpFPLTTTGKIRRDEVRREV 552
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
184-540 |
2.16e-40 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 152.45 E-value: 2.16e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 184 PDDEWDAISLnYTSGTTGNPKGVVYHHRGAAlmaytntihAGM-AKHAVYLWT--------LPMFHCNGWCFPWTLAVQA 254
Cdd:PRK07787 125 PDPDAPALIV-YTSGTTGPPKGVVLSRRAIA---------ADLdALAEAWQWTaddvlvhgLPLFHVHGLVLGVLGPLRI 194
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 255 G---THVclrwVRPKPI-YDAIADHGVTHLCGAPVVMSVLinARDED-KRTFAQTVTFNTAAAPPPEAVLSGMADA-GFA 328
Cdd:PRK07787 195 GnrfVHT----GRPTPEaYAQALSEGGTLYFGVPTVWSRI--AADPEaARALRGARLLVSGSAALPVPVFDRLAALtGHR 268
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 329 VTHLYGLTETYGPAVVnewhgewddLAKGERsakkaRQGVRYAALEGL-TVMDPETMQATPADGETIGEVMFRGNIVMKG 407
Cdd:PRK07787 269 PVERYGMTETLITLST---------RADGER-----RPGWVGLPLAGVeTRLVDEDGGPVPHDGETVGELQVRGPTLFDG 334
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 408 YLKNRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDR-SKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGE 485
Cdd:PRK07787 335 YLNRPDATAAAFtADGWFRTGDVAVVDPDGMHRIVGReSTDLIKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQ 414
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*.
gi 499217198 486 VPVAYVelKPGKTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKIQKFRLR 540
Cdd:PRK07787 415 RIVAYV--VGADDVAADELIDFVAQQLSVHKRPREVRFVDaLPRNAMGKVLKKQLL 468
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
11-541 |
1.19e-39 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 151.62 E-value: 1.19e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 11 RNAANHQPLTPLTYLERAAktypdhiaiiHGRQRISYRDFWRRSLKVASALQKRGiGKGDTVTVMLSNTPPMLEAHFGVP 90
Cdd:cd05931 1 RRAAARPDRPAYTFLDDEG----------GREETLTYAELDRRARAIAARLQAVG-KPGDRVLLLAPPGLDFVAAFLGCL 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 91 MTKAV---LHSLNTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQ--ALDLAKVTPLVIDYddpdyaadapypkgeriga 165
Cdd:cd05931 70 YAGAIavpLPPPTPGRHAERLAAILADAGPRVVLTTAAALAAVRAfaASRPAAGTPRLLVV------------------- 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 166 ldyeDFVAGGDEDfAWSMPDDEWDAIS-LNYTSGTTGNPKGVVYHHRG--AALMAYTNTIHAGMAKHAVyLWtLPMFHCN 242
Cdd:cd05931 131 ----DLLPDTSAA-DWPPPSPDPDDIAyLQYTSGSTGTPKGVVVTHRNllANVRQIRRAYGLDPGDVVV-SW-LPLYHDM 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 243 GWCFPWTLAVQAGTHVCL-----------RWVRpkpiydAIADHGVTH---------LCGAPVvmsvlinaRDEDKRTF- 301
Cdd:cd05931 204 GLIGGLLTPLYSGGPSVLmspaaflrrplRWLR------LISRYRATIsaapnfaydLCVRRV--------RDEDLEGLd 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 302 ---------------AQTVT-FNTAAAP---PPEAVLS--GMADAGFAVThlyGLTETYGPAVVnewHGEWDDLAKGERS 360
Cdd:cd05931 270 lsswrvalngaepvrPATLRrFAEAFAPfgfRPEAFRPsyGLAEATLFVS---GGPPGTGPVVL---RVDRDALAGRAVA 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 361 AKKARQGVRY-----AALEGLTV--MDPETMQATPADgeTIGEVMFRGNIVMKGYLKNRKASDEAF-------AGGWFHS 426
Cdd:cd05931 344 VAADDPAARElvscgRPLPDQEVriVDPETGRELPDG--EVGEIWVRGPSVASGYWGRPEATAETFgalaatdEGGWLRT 421
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 427 GDLGVMHpDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSV--ASCGVVARHDDKWGEVPVAYVELKPGKTASE-AE 503
Cdd:cd05931 422 GDLGFLH-DGELYITGRLKDLIIVRGRNHYPQDIEATAEEAHPAlrPGCVAAFSVPDDGEERLVVVAEVERGADPADlAA 500
|
570 580 590 600
....*....|....*....|....*....|....*....|...
gi 499217198 504 IIEHCRALLAR-FKV-PKAVIFAE---IPKTSTGKIQKFRLRE 541
Cdd:cd05931 501 IAAAIRAAVAReHGVaPADVVLVRpgsIPRTSSGKIQRRACRA 543
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
34-534 |
1.32e-39 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 151.97 E-value: 1.32e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 34 DHIAII----HGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIA 109
Cdd:PRK04319 59 DKVALRyldaSRKEKYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVR 138
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 110 FQLDHAETKVLIV-DREFSGVVRQAL-DLAKVtpLVIDYDDpdyaadapypkGERIGALDYEDFVAGGDEDFA--WSMPD 185
Cdd:PRK04319 139 DRLEDSEAKVLITtPALLERKPADDLpSLKHV--LLVGEDV-----------EEGPGTLDFNALMEQASDEFDieWTDRE 205
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 186 DEwdAIsLNYTSGTTGNPKGVVYHHrGAALMAYTNtihagmAKHAVYLWTLPMFHCN---GWCF--------PW----TL 250
Cdd:PRK04319 206 DG--AI-LHYTSGSTGKPKGVLHVH-NAMLQHYQT------GKYVLDLHEDDVYWCTadpGWVTgtsygifaPWlngaTN 275
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 251 AVQAGTHVCLRWvrpkpiYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFA-QTVTFNTAAAPP--PEAVLSGMADAGF 327
Cdd:PRK04319 276 VIDGGRFSPERW------YRILEDYKVTVWYTAPTAIRMLMGAGDDLVKKYDlSSLRHILSVGEPlnPEVVRWGMKVFGL 349
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 328 AVTHLYGLTETYG------PAVvnewhgewdDLAKGerSAKKARQGVRYAalegltVMDPETmQATPADgeTIGEV---- 397
Cdd:PRK04319 350 PIHDNWWMTETGGimianyPAM---------DIKPG--SMGKPLPGIEAA------IVDDQG-NELPPN--RMGNLaikk 409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 398 ----MFRGnivmkgYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASC 473
Cdd:PRK04319 410 gwpsMMRG------IWNNPEKYESYFAGDWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESKLMEHPAVAEA 483
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 499217198 474 GVVARHDDKWGEVPVAYVELKPGKTASEA---EIIEHCRALLARFKVPKAVIFAE-IPKTSTGKI 534
Cdd:PRK04319 484 GVIGKPDPVRGEIIKAFVALRPGYEPSEElkeEIRGFVKKGLGAHAAPREIEFKDkLPKTRSGKI 548
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
196-543 |
4.36e-39 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 145.17 E-value: 4.36e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 196 TSGTTGNPKGVVyhHRGAALMAYTNTIHA--GMAKHAVYLWTLPMFHCNGWcFPWTLAVQAGTHVCLRWVRPkPIYDAIA 273
Cdd:cd17630 8 TSGSTGTPKAVV--HTAANLLASAAGLHSrlGFGGGDSWLLSLPLYHVGGL-AILVRSLLAGAELVLLERNQ-ALAEDLA 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 274 DHGVTHLCGAPvvmSVLINARDEDKRTFAQT----VTFNTAAAPPPeaVLSGMADAGFAVTHLYGLTETyGPAVvnewhG 349
Cdd:cd17630 84 PPGVTHVSLVP---TQLQRLLDSGQGPAALKslraVLLGGAPIPPE--LLERAADRGIPLYTTYGMTET-ASQV-----A 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 350 EWDDLAKGERSAKKARQGVRYAALEGltvmdpetmqatpadgetiGEVMFRGNIVMKGYLKNRkASDEAFAGGWFHSGDL 429
Cdd:cd17630 153 TKRPDGFGRGGVGVLLPGRELRIVED-------------------GEIWVGGASLAMGYLRGQ-LVPEFNEDGWFTTKDL 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 430 GVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGktASEAEIIEHCR 509
Cdd:cd17630 213 GELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGRGP--ADPAELRAWLK 290
|
330 340 350
....*....|....*....|....*....|....*
gi 499217198 510 ALLARFKVPKAVIF-AEIPKTSTGKIQKFRLREMA 543
Cdd:cd17630 291 DKLARFKLPKRIYPvPELPRTGGGKVDRRALRAWL 325
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
189-546 |
5.37e-39 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 149.91 E-value: 5.37e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 DAISLNYTSGTTGNPKGVVYHHRG-AALMAYTNTIHAGMAKHA--VYLWTLPMFHCNGWCFPWTLAVQAGTHVCLrWVRP 265
Cdd:PRK05677 208 DVAVLQYTGGTTGVAKGAMLTHRNlVANMLQCRALMGSNLNEGceILIAPLPLYHIYAFTFHCMAMMLIGNHNIL-ISNP 286
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 266 KPIYDAIADHGVTHLCGApVVMSVLINA-------RDED----KRTFAQTVTFNTAAAPPPEAVlsgmadAGFAVTHLYG 334
Cdd:PRK05677 287 RDLPAMVKELGKWKFSGF-VGLNTLFVAlcnneafRKLDfsalKLTLSGGMALQLATAERWKEV------TGCAICEGYG 359
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 335 LTETYGPAVVNewhgewddlakgersakkARQGVRYAALeGLTVmdPETMQATPAD-------GEtIGEVMFRGNIVMKG 407
Cdd:PRK05677 360 MTETSPVVSVN------------------PSQAIQVGTI-GIPV--PSTLCKVIDDdgnelplGE-VGELCVKGPQVMKG 417
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 408 YLKNRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEV 486
Cdd:PRK05677 418 YWQRPEATDEILdSDGWLKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLAALPGVLQCAAIGVPDEKSGEA 497
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 499217198 487 PVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLR--EMAKLA 546
Cdd:PRK05677 498 IKVFVVVKPGETLTKEQVMEHMRANLTGYKVPKAVEFRdELPTTNVGKILRRELRdeELKKAG 560
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
189-533 |
2.14e-38 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 144.45 E-value: 2.14e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 DAISLNYTSGTTGNPKGVVYHHR-------GAALMAYTNTIHAGMAKHA-------VYLWTLPMFHCNGWcFPWTLAVQA 254
Cdd:cd05924 4 DDLYILYTGGTTGMPKGVMWRQEdifrmlmGGADFGTGEFTPSEDAHKAaaaaagtVMFPAPPLMHGTGS-WTAFGGLLG 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 255 GTHVCLRWVR--PKPIYDAIADHGVTHLcgaPVVMSV----LINA-RDEDKRTFAQTVTFNTAAAPPPEAVLSGMADA-- 325
Cdd:cd05924 83 GQTVVLPDDRfdPEEVWRTIEKHKVTSM---TIVGDAmarpLIDAlRDAGPYDLSSLFAISSGGALLSPEVKQGLLELvp 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 326 GFAVTHLYGLTETYGpavvnewhgewddLAKGERSAKKARQGVRYAALEGLTVMDPETMQATPADGEtIGEVMFRGNIVM 405
Cdd:cd05924 160 NITLVDAFGSSETGF-------------TGSGHSAGSGPETGPFTRANPDTVVLDDDGRVVPPGSGG-VGWIARRGHIPL 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 406 kGYLKNRKASDEAF--AGG--WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDD 481
Cdd:cd05924 226 -GYYGDEAKTAETFpeVDGvrYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDE 304
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|...
gi 499217198 482 KWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGK 533
Cdd:cd05924 305 RWGQEVVAVVQLREGAGVDLEELREHCRTRIARYKLPKQVVFVdEIERSPAGK 357
|
|
| ligase_PEP_1 |
TIGR03098 |
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ... |
25-536 |
2.59e-38 |
|
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.
Pssm-ID: 211788 [Multi-domain] Cd Length: 517 Bit Score: 147.62 E-value: 2.59e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLD 104
Cdd:TIGR03098 6 LEDAAARLPDATALVHHDRTLTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGVFVPINPLLK 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 105 AAVIAFQLDHAETKVLIVDREfsgvVRQALDLAKVTPLVIDYDDPDYAADAPYPKGERIGALDYEDFVAGGDEDFAWSMP 184
Cdd:TIGR03098 86 AEQVAHILADCNVRLLVTSSE----RLDLLHPALPGCHDLRTLIIVGDPAHASEGHPGEEPASWPKLLALGDADPPHPVI 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 185 DDEWDAISlnYTSGTTGNPKGVVYHHR----GAALMAYTNTIHAGMAKHAVylwtLPMFHCNGWCFPWTLAVQAGTHVCL 260
Cdd:TIGR03098 162 DSDMAAIL--YTSGSTGRPKGVVLSHRnlvaGAQSVATYLENRPDDRLLAV----LPLSFDYGFNQLTTAFYVGATVVLH 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 261 RWVRPKPIYDAIADHGVTHLCGAPVVMSVLinARDEDKRTFAQTVTF--NTAAAPPPEAVLSGMADAGFAVTHL-YGLTE 337
Cdd:TIGR03098 236 DYLLPRDVLKALEKHGITGLAAVPPLWAQL--AQLDWPESAAPSLRYltNSGGAMPRATLSRLRSFLPNARLFLmYGLTE 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 338 ----TYGPAvvnewhgewDDLAKGERSAKKARQGVRyaalegLTVMDPETMQATPadGEtIGEVMFRGNIVMKGYLKNRK 413
Cdd:TIGR03098 314 afrsTYLPP---------EEVDRRPDSIGKAIPNAE------VLVLREDGSECAP--GE-EGELVHRGALVAMGYWNDPE 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 414 ASDEAF------------AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDD 481
Cdd:TIGR03098 376 KTAERFrplppfpgelhlPELAVWSGDTVRRDEEGFLYFVGRRDEMIKTSGYRVSPTEVEEVAYATGLVAEAVAFGVPDP 455
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 499217198 482 KWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKIQK 536
Cdd:TIGR03098 456 TLGQAIVLVVTPPGGEELDRAALLAECRARLPNYMVPALIHVRQaLPRNANGKIDR 511
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
25-541 |
6.91e-38 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 146.49 E-value: 6.91e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIH-----GRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSL 99
Cdd:cd05970 23 VDAMAKEYPDKLALVWcddagEERIFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALHKLGAIAIPA 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 100 NTRLDAAVIAFQLDHAETKVLIVDRE--FSGVVRQALDLAKVTPLVIDYDDPdyaadapypkgERIGALDYEDFVAGGDE 177
Cdd:cd05970 103 THQLTAKDIVYRIESADIKMIVAIAEdnIPEEIEKAAPECPSKPKLVWVGDP-----------VPEGWIDFRKLIKNASP 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 178 DFAWSMPDDEW--DAISLNY-TSGTTGNPKGVVYHHR---GAALMA-YTNTIHAG-----------------------MA 227
Cdd:cd05970 172 DFERPTANSYPcgEDILLVYfSSGTTGMPKMVEHDFTyplGHIVTAkYWQNVREGglhltvadtgwgkavwgkiygqwIA 251
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 228 KHAVYLWTLPMFHcngwcfpwtlavqagthvclrwvrPKPIYDAIADHGVTHLCGAPVVMSVLINArDEDKRTFAQTVTF 307
Cdd:cd05970 252 GAAVFVYDYDKFD------------------------PKALLEKLSKYGVTTFCAPPTIYRFLIRE-DLSRYDLSSLRYC 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 308 NTAAAPPPEAVLSGMADA-GFAVTHLYGLTETygpaVVNEWHGEWDDLAKGerSAKKARQGVryaaleGLTVMDPEtmqA 386
Cdd:cd05970 307 TTAGEALNPEVFNTFKEKtGIKLMEGFGQTET----TLTIATFPWMEPKPG--SMGKPAPGY------EIDLIDRE---G 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 387 TPADGETIGEVMFR---GNIV--MKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVE 461
Cdd:cd05970 372 RSCEAGEEGEIVIRtskGKPVglFGGYYKDAEKTAEVWHDGYYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVE 451
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 462 DALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEA---EIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKF 537
Cdd:cd05970 452 SALIQHPAVLECAVTGVPDPIRGQVVKATIVLAKGYEPSEElkkELQDHVKKVTAPYKYPRIVEFvDELPKTISGKIRRV 531
|
....
gi 499217198 538 RLRE 541
Cdd:cd05970 532 EIRE 535
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
29-540 |
2.62e-37 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 144.38 E-value: 2.62e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 29 AKTYPDHIAIIHGR--QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAA 106
Cdd:PRK13390 7 AQIAPDRPAVIVAEtgEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTAP 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 107 VIAFQLDHAETKVLIVDREFSGVVRQAldlAKVTPLVIDYDdpdyaadapypkGERIGALDYEDFVAGGDEDFAwsmpDD 186
Cdd:PRK13390 87 EADYIVGDSGARVLVASAALDGLAAKV---GADLPLRLSFG------------GEIDGFGSFEAALAGAGPRLT----EQ 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 187 EWDAISLnYTSGTTGNPKGVVYHHRGAALMAYTNTIHA------GMAKHAVYLWTLPMFHCNG--WCfpWTLAVQAGTHV 258
Cdd:PRK13390 148 PCGAVML-YSSGTTGFPKGIQPDLPGRDVDAPGDPIVAiarafyDISESDIYYSSAPIYHAAPlrWC--SMVHALGGTVV 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 259 CLRWVRPKPIYDAIADHGVTHLCGAPVvMSVLINARDEDKRTFAQTVTFNT---AAAPPPEAVLSGMAD-AGFAVTHLYG 334
Cdd:PRK13390 225 LAKRFDAQATLGHVERYRITVTQMVPT-MFVRLLKLDADVRTRYDVSSLRAvihAAAPCPVDVKHAMIDwLGPIVYEYYS 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 335 LTETYGPAVVNEwhGEWddlakgersakKARQG-VRYAALEGLTVMDPETMQaTPAdGEtIGEVMFRGNIVMKGYLKNRK 413
Cdd:PRK13390 304 STEAHGMTFIDS--PDW-----------LAHPGsVGRSVLGDLHICDDDGNE-LPA-GR-IGTVYFERDRLPFRYLNDPE 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 414 ASDEAFAGG---WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAY 490
Cdd:PRK13390 368 KTAAAQHPAhpfWTTVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQVKAV 447
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 499217198 491 VELKPGKTASEA---EIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLR 540
Cdd:PRK13390 448 IQLVEGIRGSDElarELIDYTRSRIAHYKAPRSVEFVdELPRTPTGKLVKGLLR 501
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
25-534 |
6.77e-37 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 144.26 E-value: 6.77e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIH------GRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHS 98
Cdd:cd17634 59 LDRHLRENGDRTAIIYegddtsQSRTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSV 138
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 99 LNTRLDAAVIAFQLDHAETKVLIVDREF---------SGVVRQALDLAKVTPLVIdyddPDYAADAPYPKGERIGALDYE 169
Cdd:cd17634 139 IFGGFAPEAVAGRIIDSSSRLLITADGGvragrsvplKKNVDDALNPNVTSVEHV----IVLKRTGSDIDWQEGRDLWWR 214
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 170 DFVAGGDEDFAwSMPDDEWDAISLNYTSGTTGNPKGVVYHHRGAAL-----MAYTNTIHAGmakhAVYLWTLPMfhcnGW 244
Cdd:cd17634 215 DLIAKASPEHQ-PEAMNAEDPLFILYTSGTTGKPKGVLHTTGGYLVyaattMKYVFDYGPG----DIYWCTADV----GW 285
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 245 CF--PWTL--AVQAGTHVCL-----RWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDE--DKRTFAQTVTFNTAAAP 313
Cdd:cd17634 286 VTghSYLLygPLACGATTLLyegvpNWPTPARMWQVVDKHGVNILYTAPTAIRALMAAGDDaiEGTDRSSLRILGSVGEP 365
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 314 -PPEA---VLSGMADAGFAVTHLYGLTETYGPAVVNewhgewddlakgERSAKKARQGVRYAALEGL--TVMDPEtmqAT 387
Cdd:cd17634 366 iNPEAyewYWKKIGKEKCPVVDTWWQTETGGFMITP------------LPGAIELKAGSATRPVFGVqpAVVDNE---GH 430
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 388 PADGETIGEVMFRGNI--VMKGYLKNRKASDEA----FAGGWFHsGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVE 461
Cdd:cd17634 431 PQPGGTEGNLVITDPWpgQTRTLFGDHERFEQTyfstFKGMYFS-GDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIE 509
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 499217198 462 DALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASE---AEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKI 534
Cdd:cd17634 510 SVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVLNHGVEPSPelyAELRNWVRKEIGPLATPDVVHWVDsLPKTRSGKI 586
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
34-543 |
1.78e-36 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 142.47 E-value: 1.78e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 34 DHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDT-VTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQL 112
Cdd:PRK13388 16 DTIAVRYGDRTWTWREVLAEAAARAAALIALADPDRPLhVGVLLGNTPEMLFWLAAAALGGYVLVGLNTTRRGAALAADI 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 113 DHAETKVLIVDREFSGVVRqALDLAKVTPLVIdyddpdyaadapypkgeriGALDYEDFVAGgDEDFAWSMPDDEWDAIS 192
Cdd:PRK13388 96 RRADCQLLVTDAEHRPLLD-GLDLPGVRVLDV-------------------DTPAYAELVAA-AGALTPHREVDAMDPFM 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 193 LNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLRwvrpkPIYDA- 271
Cdd:PRK13388 155 LIFTSGTTGAPKAVRCSHGRLAFAGRALTERFGLTRDDVCYVSMPLFHSNAVMAGWAPAVASGAAVALP-----AKFSAs 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 272 -----IADHGVTHL--CGAPV--VMSVLINARDEDKRTfaqTVTFNTAAAPPPEAVLSgmADAGFAVTHLYGLTETyGPA 342
Cdd:PRK13388 230 gflddVRRYGATYFnyVGKPLayILATPERPDDADNPL---RVAFGNEASPRDIAEFS--RRFGCQVEDGYGSSEG-AVI 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 343 VVNEwhgewddlakgersaKKARQGVRYAALEGLTVMDPETMQATP-----ADG------ETIGE-VMFRGNIVMKGYLK 410
Cdd:PRK13388 304 VVRE---------------PGTPPGSIGRGAPGVAIYNPETLTECAvarfdAHGallnadEAIGElVNTAGAGFFEGYYN 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 411 NRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAY 490
Cdd:PRK13388 369 NPEATAERMRHGMYWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDERVGDQVMAA 448
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 499217198 491 VELKPGKTASEAEIIEHCRAL--LARFKVPKAV-IFAEIPKTSTGKIQKFRLREMA 543
Cdd:PRK13388 449 LVLRDGATFDPDAFAAFLAAQpdLGTKAWPRYVrIAADLPSTATNKVLKRELIAQG 504
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
45-540 |
3.12e-36 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 142.29 E-value: 3.12e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 45 ISYRDFWRRSLKVASALQKR-GIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVD 123
Cdd:PLN02574 67 ISYSELQPLVKSMAAGLYHVmGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSVGLAFTS 146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 124 REFSGvvrqaldlaKVTPLVIDYddpdyaadapypkgerIGALDYEDFVAGGDED--FAWSMPDD----------EWDAI 191
Cdd:PLN02574 147 PENVE---------KLSPLGVPV----------------IGVPENYDFDSKRIEFpkFYELIKEDfdfvpkpvikQDDVA 201
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 192 SLNYTSGTTGNPKGVVYHHRGAALMA-----YTNTIHAGMAKHAVYLWTLPMFHCNGWC-FPWTLAVQAGTHVCLRWVRP 265
Cdd:PLN02574 202 AIMYSSGTTGASKGVVLTHRNLIAMVelfvrFEASQYEYPGSDNVYLAALPMFHIYGLSlFVVGLLSLGSTIVVMRRFDA 281
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 266 KPIYDAIADHGVTHLCGAPVVMSVLIN-ARDEDKRTFAQTVTFNTAAAPppeavLSGMADAGF--AVTHL-----YGLTE 337
Cdd:PLN02574 282 SDMVKVIDRFKVTHFPVVPPILMALTKkAKGVCGEVLKSLKQVSCGAAP-----LSGKFIQDFvqTLPHVdfiqgYGMTE 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 338 TygpavvnewhgewddLAKGER--SAKKARqgvRYAALeGLtvMDPeTMQATPADGET--------IGEVMFRGNIVMKG 407
Cdd:PLN02574 357 S---------------TAVGTRgfNTEKLS---KYSSV-GL--LAP-NMQAKVVDWSTgcllppgnCGELWIQGPGVMKG 414
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 408 YLKNRKASD-EAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEV 486
Cdd:PLN02574 415 YLNNPKATQsTIDKDGWLRTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEI 494
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 499217198 487 PVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKIQKFRLR 540
Cdd:PLN02574 495 PVAFVVRRQGSTLSQEAVINYVAKQVAPYKKVRKVVFVQsIPKSPAGKILRRELK 549
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
25-541 |
7.56e-35 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 138.47 E-value: 7.56e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVpmTK--AVLHSLNTR 102
Cdd:PRK08279 43 FEEAAARHPDRPALLFEDQSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGL--AKlgAVVALLNTQ 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 103 LDAAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVidyddpdyAADAPYPKGERIGALDYEDFVAGGDEDfaws 182
Cdd:PRK08279 121 QRGAVLAHSLNLVDAKHLIVGEELVEAFEEARADLARPPRL--------WVAGGDTLDDPEGYEDLAAAAAGAPTT---- 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 183 mPDDEWDAISLN------YTSGTTGNPKGVVYHHRGAALM--AYTNTIHAGmAKHAVYLwTLPMFHCNGWCFPWTLAVQA 254
Cdd:PRK08279 189 -NPASRSGVTAKdtafyiYTSGTTGLPKAAVMSHMRWLKAmgGFGGLLRLT-PDDVLYC-CLPLYHNTGGTVAWSSVLAA 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 255 GTHVCLR--------WvrpkpiyDAIADHGVTH----------LCGAPvvmsvlinARDEDKRTFAQTVTFNtaaapppe 316
Cdd:PRK08279 266 GATLALRrkfsasrfW-------DDVRRYRATAfqyigelcryLLNQP--------PKPTDRDHRLRLMIGN-------- 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 317 avlsgmadagfavthlyGLTetygPAVvnewhgeWDDLakgersakKARQGVR-----YAALEGLTVM------------ 379
Cdd:PRK08279 323 -----------------GLR----PDI-------WDEF--------QQRFGIPrilefYAASEGNVGFinvfnfdgtvgr 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 380 --------------DPET----------MQATPAD--GETIGEVMFRGNivMKGYLkNRKASD-----EAFAGG--WFHS 426
Cdd:PRK08279 367 vplwlahpyaivkyDVDTgepvrdadgrCIKVKPGevGLLIGRITDRGP--FDGYT-DPEASEkkilrDVFKKGdaWFNT 443
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 427 GDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASC---GV-VARHDDKWGevpVAYVELKPGKTASEA 502
Cdd:PRK08279 444 GDLMRDDGFGHAQFVDRLGDTFRWKGENVATTEVENALSGFPGVEEAvvyGVeVPGTDGRAG---MAAIVLADGAEFDLA 520
|
570 580 590 600
....*....|....*....|....*....|....*....|
gi 499217198 503 EIIEHCRALLARFKVPKAV-IFAEIPKTSTGKIQKFRLRE 541
Cdd:PRK08279 521 ALAAHLYERLPAYAVPLFVrLVPELETTGTFKYRKVDLRK 560
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
25-544 |
2.17e-34 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 136.69 E-value: 2.17e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLD 104
Cdd:PRK07059 29 LEESFRQYADRPAFICMGKAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGYVVVNVNPLYT 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 105 AAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVIDYDDPDYAAdapypKG-------ERI----------GALD 167
Cdd:PRK07059 109 PRELEHQLKDSGAEAIVVLENFATTVQQVLAKTAVKHVVVASMGDLLGF-----KGhivnfvvRRVkkmvpawslpGHVR 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 168 YEDFVA-GGDEDFAWSM--PDDewdAISLNYTSGTTGNPKGVVYHHRGaaLMAYTNTIHAGMA---------KHAVYLWT 235
Cdd:PRK07059 184 FNDALAeGARQTFKPVKlgPDD---VAFLQYTGGTTGVSKGATLLHRN--IVANVLQMEAWLQpafekkprpDQLNFVCA 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 236 LPMFHCngwcFPWT----LAVQAGTHVCLrWVRPKPI---------YDAIADHGVTHLCGApvvmsvLINARDEDKRTFA 302
Cdd:PRK07059 259 LPLYHI----FALTvcglLGMRTGGRNIL-IPNPRDIpgfikelkkYQVHIFPAVNTLYNA------LLNNPDFDKLDFS 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 303 QTVTFNTAAApppeAVLSGMADAGFAVTHL-----YGLTETYGPAVVNewhgewddlakgersakkARQGVRYAALEGLT 377
Cdd:PRK07059 328 KLIVANGGGM----AVQRPVAERWLEMTGCpitegYGLSETSPVATCN------------------PVDATEFSGTIGLP 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 378 VmdPETMQATPAD-------GEtIGEVMFRGNIVMKGYLkNRkaSDEA----FAGGWFHSGDLGVMHPDGYIQLKDRSKD 446
Cdd:PRK07059 386 L--PSTEVSIRDDdgndlplGE-PGEICIRGPQVMAGYW-NR--PDETakvmTADGFFRTGDVGVMDERGYTKIVDRKKD 459
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 447 IIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVeLKPGKTASEAEIIEHCRALLARFKVPKAVIF-AE 525
Cdd:PRK07059 460 MILVSGFNVYPNEIEEVVASHPGVLEVAAVGVPDEHSGEAVKLFV-VKKDPALTEEDVKAFCKERLTNYKRPKFVEFrTE 538
|
570
....*....|....*....
gi 499217198 526 IPKTSTGKIQKFRLREMAK 544
Cdd:PRK07059 539 LPKTNVGKILRRELRDGKA 557
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
29-539 |
8.34e-34 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 133.53 E-value: 8.34e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 29 AKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVI 108
Cdd:cd05945 1 AAANPDRPAVVEGGRTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAERI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 109 AFQLDHAETKVLIVDrefsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvagGDEDFAwsmpddew 188
Cdd:cd05945 81 REILDAAKPALLIAD---------------------------------------------------GDDNAY-------- 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 daisLNYTSGTTGNPKGVVYHHRgaALMAYTNTI--HAGMAKHAVYLWTLPmFH---CNGWCFPwTLAVqAGTHVCLR-- 261
Cdd:cd05945 102 ----IIFTSGSTGRPKGVQISHD--NLVSFTNWMlsDFPLGPGDVFLNQAP-FSfdlSVMDLYP-ALAS-GATLVPVPrd 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 262 -WVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADA--GFAVTHLYGLTET 338
Cdd:cd05945 173 aTADPKQLFRFLAEHGITVWVSTPSFAAMCLLSPTFTPESLPSLRHFLFCGEVLPHKTARALQQRfpDARIYNTYGPTEA 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 339 YGPAVVNEWHGEwdDLAKGER-SAKKARQGVRyaalegLTVMDPETMQATPADgetIGEVMFRGNIVMKGYLKNRKASDE 417
Cdd:cd05945 253 TVAVTYIEVTPE--VLDGYDRlPIGYAKPGAK------LVILDEDGRPVPPGE---KGELVISGPSVSKGYLNNPEKTAA 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 418 AF----AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVEL 493
Cdd:cd05945 322 AFfpdeGQRAYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALRQVPGVKEAVVVPKYKGEKVTELIAFVVP 401
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 499217198 494 KPGKTAS-EAEIIEHCRALLARFKVP-KAVIFAEIPKTSTGKIQKFRL 539
Cdd:cd05945 402 KPGAEAGlTKAIKAELAERLPPYMIPrRFVYLDELPLNANGKIDRKAL 449
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
189-543 |
6.16e-33 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 132.64 E-value: 6.16e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 DAISLNYTSGTTGNPKGVVYHHrgAALMAYTNTIHAGMAKHA------------VYLWTLPMFHCNGWCFPWTLAVQAGT 256
Cdd:PRK12492 208 DIAVLQYTGGTTGLAKGAMLTH--GNLVANMLQVRACLSQLGpdgqplmkegqeVMIAPLPLYHIYAFTANCMCMMVSGN 285
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 257 HVCLrWVRPKPIYDAIADHG---VTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAApppeAVLSGMAD-----AGFA 328
Cdd:PRK12492 286 HNVL-ITNPRDIPGFIKELGkwrFSALLGLNTLFVALMDHPGFKDLDFSALKLTNSGGT----ALVKATAErweqlTGCT 360
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 329 VTHLYGLTETyGPAVVNEWHGEwddlakgersakKARQGVRYAALEG--LTVMDPETMQAtpADGETiGEVMFRGNIVMK 406
Cdd:PRK12492 361 IVEGYGLTET-SPVASTNPYGE------------LARLGTVGIPVPGtaLKVIDDDGNEL--PLGER-GELCIKGPQVMK 424
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 407 GYLKNRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGE 485
Cdd:PRK12492 425 GYWQQPEATAEALdAEGWFKTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYPNEIEDVVMAHPKVANCAAIGVPDERSGE 504
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*....
gi 499217198 486 VPVAYVELKPGKTASEaEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKIQKFRLREMA 543
Cdd:PRK12492 505 AVKLFVVARDPGLSVE-ELKAYCKENFTGYKVPKHIVLRDsLPMTPVGKILRRELRDIA 562
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
25-536 |
1.45e-32 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 131.16 E-value: 1.45e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRI--SYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTR 102
Cdd:PRK05852 22 VEVAATRLPEAPALVVTADRIaiSYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLVVVPLDPA 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 103 LDAAVIAFQLDHAETKVLIVDREfsGVVRQALDLAKVTPLVIDYDDPdyaadapypKGERIGALDYeDFVAGGDEDFAWS 182
Cdd:PRK05852 102 LPIAEQRVRSQAAGARVVLIDAD--GPHDRAEPTTRWWPLTVNVGGD---------SGPSGGTLSV-HLDAATEPTPATS 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 183 MPDD--EWDAISLnYTSGTTGNPKGVVYHHrgAALMAYTNTIHAG--MAKHAVYLWTLPMFHCNGwCFPWTLAVQAGTHV 258
Cdd:PRK05852 170 TPEGlrPDDAMIM-FTGGTTGLPKMVPWTH--ANIASSVRAIITGyrLSPRDATVAVMPLYHGHG-LIAALLATLASGGA 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 259 CLRWVRPK----PIYDAIADHGVTHLCGAPVVMSVLIN-ARDEDKRTFAQTVTFNTAAAPP--PEAVLSGMADAGFAVTH 331
Cdd:PRK05852 246 VLLPARGRfsahTFWDDIKAVGATWYTAVPTIHQILLErAATEPSGRKPAALRFIRSCSAPltAETAQALQTEFAAPVVC 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 332 LYGLTETYGPAVVNEWHGEWDDLAKGERSAKKARQG---VRYAALEGltvmdpetmQATPADgeTIGEVMFRGNIVMKGY 408
Cdd:PRK05852 326 AFGMTEATHQVTTTQIEGIGQTENPVVSTGLVGRSTgaqIRIVGSDG---------LPLPAG--AVGEVWLRGTTVVRGY 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 409 LKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPV 488
Cdd:PRK05852 395 LGDPTITAANFTDGWLRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGEAVA 474
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 499217198 489 AYVELKPGKTASEAEIIEHCRALLARFKVPKAV-IFAEIPKTSTGKIQK 536
Cdd:PRK05852 475 AVIVPRESAPPTAEELVQFCRERLAAFEIPASFqEASGLPHTAKGSLDR 523
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
26-539 |
2.19e-32 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 130.01 E-value: 2.19e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 26 ERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDA 105
Cdd:cd12117 4 EEQAARTPDAVAVVYGDRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPELPA 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 106 AVIAFQLDHAETKVLIVDREFSGVVrqaldlakvtplvidyddpdyaadapypkGERIGALDYEDFVAGGDEDfAWSMPD 185
Cdd:cd12117 84 ERLAFMLADAGAKVLLTDRSLAGRA-----------------------------GGLEVAVVIDEALDAGPAG-NPAVPV 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 186 DEWDAISLNYTSGTTGNPKGVVYHHRG-AALMAYTNTIHAG----MAKHAVYLW---TLPMFHC--NGWCfpwtLAVQAG 255
Cdd:cd12117 134 SPDDLAYVMYTSGSTGRPKGVAVTHRGvVRLVKNTNYVTLGpddrVLQTSPLAFdasTFEIWGAllNGAR----LVLAPK 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 256 THVclrwVRPKPIYDAIADHGVThlcgapvVM---SVLINA-RDEDKRTFAQ--TVTFNTAAAPPP--EAVLSgmADAGF 327
Cdd:cd12117 210 GTL----LDPDALGALIAEEGVT-------VLwltAALFNQlADEDPECFAGlrELLTGGEVVSPPhvRRVLA--ACPGL 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 328 AVTHLYGLTETygpAVVNEWHgEWDDLAKGERSAKKARqgvryaALEGLT--VMDpETMQATPAdGETiGEVMFRGNIVM 405
Cdd:cd12117 277 RLVNGYGPTEN---TTFTTSH-VVTELDEVAGSIPIGR------PIANTRvyVLD-EDGRPVPP-GVP-GELYVGGDGLA 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 406 KGYLKNRKASDE-----AFAGG--WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVAR 478
Cdd:cd12117 344 LGYLNRPALTAErfvadPFGPGerLYRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRAHPGVREAVVVVR 423
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 499217198 479 HDDKWGEVPVAYVELKPGKTAseAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRL 539
Cdd:cd12117 424 EDAGGDKRLVAYVVAEGALDA--AELRAFLRERLPAYMVPAAFVVlDELPLTANGKVDRRAL 483
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
52-540 |
2.28e-32 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 129.48 E-value: 2.28e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 52 RRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKA----VLHSLNTRLDAAVIAFQLDHAETKVLIVDREFS 127
Cdd:cd05922 1 LGVSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGrlglVFVPLNPTLKESVLRYLVADAGGRIVLADAGAA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 128 GVVRQALDLAKVTPLVIdyddpdyaadapypkgerigalDYEDFVAGGDEDFAWSMPDDewDAISLNYTSGTTGNPKGVV 207
Cdd:cd05922 81 DRLRDALPASPDPGTVL----------------------DADGIRAARASAPAHEVSHE--DLALLLYTSGSTGSPKLVR 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 208 YHHRgaALMAYTNTIHAGMAKHA--VYLWTLPMFHCNGWCFPWTLAVQAGTHVC-LRWVRPKPIYDAIADHGVTHLCGAP 284
Cdd:cd05922 137 LSHQ--NLLANARSIAEYLGITAddRALTVLPLSYDYGLSVLNTHLLRGATLVLtNDGVLDDAFWEDLREHGATGLAGVP 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 285 VVMSVLIN-ARDEDK----RTFAQtvtfntAAAPPPEAVLSGMADA--GFAVTHLYGLTE-----TYGPAvvnewhgewD 352
Cdd:cd05922 215 STYAMLTRlGFDPAKlpslRYLTQ------AGGRLPQETIARLRELlpGAQVYVMYGQTEatrrmTYLPP---------E 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 353 DLAKGERSAKKARQGVRyaalegLTVMDPETMQATPadGEtIGEVMFRGNIVMKGYLkNRKA--SDEAFAGGWFHSGDLG 430
Cdd:cd05922 280 RILEKPGSIGLAIPGGE------FEILDDDGTPTPP--GE-PGEIVHRGPNVMKGYW-NDPPyrRKEGRGGGVLHTGDLA 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 431 VMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARhDDKWGEVPVAYVELKPGKTASeaEIIEHCRA 510
Cdd:cd05922 350 RRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGL-PDPLGEKLALFVTAPDKIDPK--DVLRSLAE 426
|
490 500 510
....*....|....*....|....*....|.
gi 499217198 511 LLARFKVPKAVIFA-EIPKTSTGKIQKFRLR 540
Cdd:cd05922 427 RLPPYKVPATVRVVdELPLTASGKVDYAALR 457
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
24-542 |
3.57e-32 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 129.95 E-value: 3.57e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 24 YLERAAKtYPDHIAII--HGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTP----PMLEAHF-GV---PMT- 92
Cdd:cd17642 23 AMKRYAS-VPGTIAFTdaHTGVNYSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLqfflPVIAGLFiGVgvaPTNd 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 93 ----KAVLHSLNtrLDAAVIAFQLDHAETKVLIVDREFSGVvrqaldlAKVTPLVIDYDDPDYAADAPYPKGERIGALDY 168
Cdd:cd17642 102 iyneRELDHSLN--ISKPTIVFCSKKGLQKVLNVQKKLKII-------KTIIILDSKEDYKGYQCLYTFITQNLPPGFNE 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 169 EDFV---AGGDEDFAWSMpddewdaislnYTSGTTGNPKGVVYHHRGAALM-------AYTNTIHAGMAkhavYLWTLPM 238
Cdd:cd17642 173 YDFKppsFDRDEQVALIM-----------NSSGSTGLPKGVQLTHKNIVARfshardpIFGNQIIPDTA----ILTVIPF 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 239 FHCNGwCFPWTLAVQAGTHVCLRW-VRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEA 317
Cdd:cd17642 238 HHGFG-MFTTLGYLICGFRVVLMYkFEEELFLRSLQDYKVQSALLVPTLFAFFAKSTLVDKYDLSNLHEIASGGAPLSKE 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 318 VLSGMADAgFAVTHL---YGLTETYGPAVVNEwhgEWDDlakgersaKKARQGVRYAALEGlTVMDPETMQATPADGEti 394
Cdd:cd17642 317 VGEAVAKR-FKLPGIrqgYGLTETTSAILITP---EGDD--------KPGAVGKVVPFFYA-KVVDLDTGKTLGPNER-- 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 395 GEVMFRGNIVMKGYLKNRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASC 473
Cdd:cd17642 382 GELCVKGPMIMKGYVNNPEATKALIdKDGWLHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDA 461
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 499217198 474 GVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFK-VPKAVIFA-EIPKTSTGKIQKFRLREM 542
Cdd:cd17642 462 GVAGIPDEDAGELPAAVVVLEAGKTMTEKEVMDYVASQVSTAKrLRGGVKFVdEVPKGLTGKIDRRKIREI 532
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
37-541 |
4.76e-32 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 129.42 E-value: 4.76e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 37 AIIHGRQRISYRDFWRRSLKVASALQKR-GIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHA 115
Cdd:PRK07867 21 GLYFEDSFTSWREHIRGSAARAAALRARlDPTRPPHVGVLLDNTPEFSLLLGAAALSGIVPVGLNPTRRGAALARDIAHA 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 116 ETKVLIVDREFSGVVRqalDLAKVTPLVidyddpdyaadapypkgeRIGALDYEDFVA---GGDEDFAWSMPDDewdAIS 192
Cdd:PRK07867 101 DCQLVLTESAHAELLD---GLDPGVRVI------------------NVDSPAWADELAahrDAEPPFRVADPDD---LFM 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 193 LNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLRwvRPKPIYDAI 272
Cdd:PRK07867 157 LIFTSGTSGDPKAVRCTHRKVASAGVMLAQRFGLGPDDVCYVSMPLFHSNAVMAGWAVALAAGASIALR--RKFSASGFL 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 273 AD---HGVTHL--CGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAVlsgMADAGFAVTHLYGLTETygpAVVNEW 347
Cdd:PRK07867 235 PDvrrYGATYAnyVGKPLSYVLATPERPDDADNPLRIVYGNEGAPGDIARF---ARRFGCVVVDGFGSTEG---GVAITR 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 348 hgewddlakgersAKKARQGVRYAALEGLTVMDPETMQATP-----ADG-----ETIGE-VMFRGNIVMKGYLKNRKASD 416
Cdd:PRK07867 309 -------------TPDTPPGALGPLPPGVAIVDPDTGTECPpaedaDGRllnadEAIGElVNTAGPGGFEGYYNDPEADA 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 417 EAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPG 496
Cdd:PRK07867 376 ERMRGGVYWSGDLAYRDADGYAYFAGRLGDWMRVDGENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQVMAALVLAPG 455
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 499217198 497 KTASEAEIIE--HCRALLARFKVPKAV-IFAEIPKTSTGKIQKFRLRE 541
Cdd:PRK07867 456 AKFDPDAFAEflAAQPDLGPKQWPSYVrVCAELPRTATFKVLKRQLSA 503
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
33-539 |
6.52e-32 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 128.18 E-value: 6.52e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 33 PDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQL 112
Cdd:cd12116 1 PDATAVRDDDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLRYIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 113 DHAETKVLIVDREfsgvvrqALDLAKVTPLVIdyddpdyaadapypkgerigALDYEDFVAGGDEdfAWSMPDDEwDAIS 192
Cdd:cd12116 81 EDAEPALVLTDDA-------LPDRLPAGLPVL--------------------LLALAAAAAAPAA--PRTPVSPD-DLAY 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 193 LNYTSGTTGNPKGVVYHHRGAA--LMAYTNTIHAGMAKHAVYLwTLPMFHCNGWCFPWTLAVQAGTHVCLRWVR--PKPI 268
Cdd:cd12116 131 VIYTSGSTGRPKGVVVSHRNLVnfLHSMRERLGLGPGDRLLAV-TTYAFDISLLELLLPLLAGARVVIAPRETQrdPEAL 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 269 YDAIADHGVTHLCGAPVVMSVLINARDEDKRTFaqTVTFNTAAAPPPEAvlSGMADAGFAVTHLYGLTETYGPAVVNEWH 348
Cdd:cd12116 210 ARLIEAHSITVMQATPATWRMLLDAGWQGRAGL--TALCGGEALPPDLA--ARLLSRVGSLWNLYGPTETTIWSTAARVT 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 349 GEWDDLAKGERSAkkarqGVRYAALEgltvmdpETMQATPAdGEtIGEVMFRGNIVMKGYLKNRKASDEAF-------AG 421
Cdd:cd12116 286 AAAGPIPIGRPLA-----NTQVYVLD-------AALRPVPP-GV-PGELYIGGDGVAQGYLGRPALTAERFvpdpfagPG 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 422 G-WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVpVAYVELKPGKTAS 500
Cdd:cd12116 352 SrLYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEAALAAHPGVAQAAVVVREDGGDRRL-VAYVVLKAGAAPD 430
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 499217198 501 EAEIIEHCRALLARFKVPKAVI-FAEIPKTSTGKIQKFRL 539
Cdd:cd12116 431 AAALRAHLRATLPAYMVPSAFVrLDALPLTANGKLDRKAL 470
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
26-534 |
8.91e-32 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 128.16 E-value: 8.91e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 26 ERAAKTyPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDA 105
Cdd:cd17646 6 EQAART-PDAPAVVDEGRTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDPGYPA 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 106 AVIAFQLDHAETKVLIVDREFSGVVRQALDLAkvtplvidyddpdyaadapypkgerigALDYEDFVAGGDEDFAWSMPD 185
Cdd:cd17646 85 DRLAYMLADAGPAVVLTTADLAARLPAGGDVA---------------------------LLGDEALAAPPATPPLVPPRP 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 186 DewDAISLNYTSGTTGNPKGVVYHHRG-AALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAgthvCLRWVR 264
Cdd:cd17646 138 D--NLAYVIYTSGSTGRPKGVMVTHAGiVNRLLWMQDEYPLGPGDRVLQKTPLSFDVSVWELFWPLVAGA----RLVVAR 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 265 P----KPIY--DAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVtFNTAAAPPPEAVLSGMADAGFAVTHLYGLTEt 338
Cdd:cd17646 212 PgghrDPAYlaALIREHGVTTCHFVPSMLRVFLAEPAAGSCASLRRV-FCSGEALPPELAARFLALPGAELHNLYGPTE- 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 339 ygpAVVNEWHGEWDDLAKGER-SAKKARQGVRyaalegLTVMDPEtMQATPADgeTIGEVMFRGNIVMKGYLkNRKA-SD 416
Cdd:cd17646 290 ---AAIDVTHWPVRGPAETPSvPIGRPVPNTR------LYVLDDA-LRPVPVG--VPGELYLGGVQLARGYL-GRPAlTA 356
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 417 EAFAGGWF-------HSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVA 489
Cdd:cd17646 357 ERFVPDPFgpgsrmyRTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHPAVTHAVVVARAAPAGAARLVG 436
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 499217198 490 YVELKPGKTA-SEAEIIEHCRALLARFKVPKA-VIFAEIPKTSTGKI 534
Cdd:cd17646 437 YVVPAAGAAGpDTAALRAHLAERLPEYMVPAAfVVLDALPLTANGKL 483
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
45-543 |
3.12e-31 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 125.76 E-value: 3.12e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 45 ISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAhfgvpMTKAVlhslntRLDAAVIAfqldhaETKVLIVDR 124
Cdd:cd05974 1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEA-----MLAAM------KLGAVVIP------ATTLLTPDD 63
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 125 efsgvVRQALDLAKVTplvidyddpdyaadapypkgerigaldyedfVAGGDEDFAWSmpddewDAISLNYTSGTTGNPK 204
Cdd:cd05974 64 -----LRDRVDRGGAV-------------------------------YAAVDENTHAD------DPMLLYFTSGTTSKPK 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 205 GVVYHHRG-----AALMAYT---------NTIHAGMAKHAvylWTlpmfhcngwCF--PWTlaVQAgTHVCLRWVR--PK 266
Cdd:cd05974 102 LVEHTHRSypvghLSTMYWIglkpgdvhwNISSPGWAKHA---WS---------CFfaPWN--AGA-TVFLFNYARfdAK 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 267 PIYDAIADHGVTHLCGAPVVMSVLINardEDKRTFAQTVTFNTAAAPP--PEAVLSGMADAGFAVTHLYGLTETygPAVV 344
Cdd:cd05974 167 RVLAALVRYGVTTLCAPPTVWRMLIQ---QDLASFDVKLREVVGAGEPlnPEVIEQVRRAWGLTIRDGYGQTET--TALV 241
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 345 NEWHGEwdDLAKGerSAKKARQGVRYAalegltVMDPETMQATpaDGET---IGEVMFRGniVMKGYLKNRKASDEAFAG 421
Cdd:cd05974 242 GNSPGQ--PVKAG--SMGRPLPGYRVA------LLDPDGAPAT--EGEValdLGDTRPVG--LMKGYAGDPDKTAHAMRG 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 422 GWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASE 501
Cdd:cd05974 308 GYYRTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLRAGYEPSP 387
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 499217198 502 A---EIIEHCRALLARFKVPKAVIFAEIPKTSTGKIQKFRLREMA 543
Cdd:cd05974 388 EtalEIFRFSRERLAPYKRIRRLEFAELPKTISGKIRRVELRRRE 432
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
25-540 |
3.71e-31 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 127.61 E-value: 3.71e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQR-----ISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSL 99
Cdd:cd05968 67 LDKWLADTRTRPALRWEGEDgtsrtLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPI 146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 100 NTRLDAAVIAFQLDHAETKVLIVDREFS---------GVVRQALDLAKVTPLVIDYDDPDYAADAPYPkgeriGALDYED 170
Cdd:cd05968 147 FSGFGKEAAATRLQDAEAKALITADGFTrrgrevnlkEEADKACAQCPTVEKVVVVRHLGNDFTPAKG-----RDLSYDE 221
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 171 FVAGGDEDFAWSMPDDEWDAIslnYTSGTTGNPKGVVYHHRGAALMAYTNTIHA-GMAKHAVYLWTLPMfhcnGWCF-PW 248
Cdd:cd05968 222 EKETAGDGAERTESEDPLMII---YTSGTTGKPKGTVHVHAGFPLKAAQDMYFQfDLKPGDLLTWFTDL----GWMMgPW 294
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 249 TL---AVQAGTHVCLRWV--RPKP--IYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQT---VTFNTAAAPPPEA- 317
Cdd:cd05968 295 LIfggLILGATMVLYDGApdHPKAdrLWRMVEDHEITHLGLSPTLIRALKPRGDAPVNAHDLSslrVLGSTGEPWNPEPw 374
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 318 --VLSGMADAGFAVTHLYGLTETYGPAVvnewhgewddlakGERSAKKARQGVRYAALEGL--TVMDPETMQATPADGET 393
Cdd:cd05968 375 nwLFETVGKGRNPIINYSGGTEISGGIL-------------GNVLIKPIKPSSFNGPVPGMkaDVLDESGKPARPEVGEL 441
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 394 IGEVMFRGniVMKGYLKNRKASDEA----FAGGWFHsGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPS 469
Cdd:cd05968 442 VLLAPWPG--MTRGFWRDEDRYLETywsrFDNVWVH-GDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPA 518
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 499217198 470 VASCGVVARHDDKWGEVPVAYVELKPGKTASEA---EIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLR 540
Cdd:cd05968 519 VLESAAIGVPHPVKGEAIVCFVVLKPGVTPTEAlaeELMERVADELGKPLSPERILFVkDLPKTRNAKVMRRVIR 593
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
33-536 |
7.07e-31 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 125.11 E-value: 7.07e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 33 PDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQL 112
Cdd:cd17643 1 PEAVAVVDEDRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVERIAFIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 113 DHAETKVLIVDrefsgvvrqALDLAKVTplvidyddpdyaadapypkgerigaldyedfvaggdedfawsmpddewdais 192
Cdd:cd17643 81 ADSGPSLLLTD---------PDDLAYVI---------------------------------------------------- 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 193 lnYTSGTTGNPKGVVYHHRG-AALMAYTNtihAGMAKHAVYLWTlpMFHCNGWCFP----WTLAVQAGTHVCL-RWVRPK 266
Cdd:cd17643 100 --YTSGSTGRPKGVVVSHANvLALFAATQ---RWFGFNEDDVWT--LFHSYAFDFSvweiWGALLHGGRLVVVpYEVARS 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 267 P--IYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFA--QTVTFNTAAAPPpeAVLSG----MADAGFAVTHLYGLTET 338
Cdd:cd17643 173 PedFARLLRDEGVTVLNQTPSAFYQLVEAADRDGRDPLalRYVIFGGEALEA--AMLRPwagrFGLDRPQLVNMYGITET 250
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 339 ygpAVVNEWHG-EWDDLAKGERSAKkarqGVRYAALeGLTVMDpETMQATPADGetIGEVMFRGNIVMKGYLkNRKA--- 414
Cdd:cd17643 251 ---TVHVTFRPlDAADLPAAAASPI----GRPLPGL-RVYVLD-ADGRPVPPGV--VGELYVSGAGVARGYL-GRPElta 318
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 415 ----SDEAFAGG--WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPV 488
Cdd:cd17643 319 erfvANPFGGPGsrMYRTGDLARRLPDGELEYLGRADEQVKIRGFRIELGEIEAALATHPSVRDAAVIVREDEPGDTRLV 398
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 499217198 489 AYVELKPGKTASEAEIIEHCRALLARFKVPKA-VIFAEIPKTSTGKIQK 536
Cdd:cd17643 399 AYVVADDGAAADIAELRALLKELLPDYMVPARyVPLDALPLTVNGKLDR 447
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
29-544 |
8.91e-31 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 125.28 E-value: 8.91e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 29 AKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGiGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVI 108
Cdd:PRK07638 11 ASLQPNKIAIKENDRVLTYKDWFESVCKVANWLNEKE-SKNKTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDEL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 109 AFQLDHAETKVLIVDREFSGvvrqalDLAKVTPLVIDYDDPDYAADAPYPKGERIGALDYEDFVAGgdedfawsmpddew 188
Cdd:PRK07638 90 KERLAISNADMIVTERYKLN------DLPDEEGRVIEIDEWKRMIEKYLPTYAPIENVQNAPFYMG-------------- 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 daislnYTSGTTGNPKGVVYHHRgAALMAYTNTIH------------AGMAKHAVYLWTlpmfhcngwcfpwtlAVQA-- 254
Cdd:PRK07638 150 ------FTSGSTGKPKAFLRAQQ-SWLHSFDCNVHdfhmkredsvliAGTLVHSLFLYG---------------AISTly 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 255 -GTHVCL-RWVRPKPIYDAIADHGVTHLCGAPVVMSVLINArdedKRTFAQTVTFNTAAAP-PPEAVlsGMADAGFAVTH 331
Cdd:PRK07638 208 vGQTVHLmRKFIPNQVLDKLETENISVMYTVPTMLESLYKE----NRVIENKMKIISSGAKwEAEAK--EKIKNIFPYAK 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 332 LYgltETYGPA---VVNEWHGEwdDLAKGERSAKKARQGVRYAALEGltvmDPETMQAtpadGEtIGEVMFRGNIVMKGY 408
Cdd:PRK07638 282 LY---EFYGASelsFVTALVDE--ESERRPNSVGRPFHNVQVRICNE----AGEEVQK----GE-IGTVYVKSPQFFMGY 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 409 LKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPV 488
Cdd:PRK07638 348 IIGGVLARELNADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGEKPV 427
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 499217198 489 AYVElkpgKTASEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLREMAK 544
Cdd:PRK07638 428 AIIK----GSATKQQLKSFCLQRLSSFKIPKEWHFVdEIPYTNSGKIARMEAKSWIE 480
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
25-541 |
7.72e-30 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 123.24 E-value: 7.72e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKR-GIGKGDTVTVMLSNtppMLE---AHFGVPMTKAVLHSLN 100
Cdd:PRK08974 29 FEQAVARYADQPAFINMGEVMTFRKLEERSRAFAAYLQNGlGLKKGDRVALMMPN---LLQypiALFGILRAGMIVVNVN 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 101 TRLDAAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVIDyddpdyaadapypkgeRIGalDYEDFVAGGDEDF- 179
Cdd:PRK08974 106 PLYTPRELEHQLNDSGAKAIVIVSNFAHTLEKVVFKTPVKHVILT----------------RMG--DQLSTAKGTLVNFv 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 180 ---------------AWSM--------------PDDEWDAIS-LNYTSGTTGNPKGVVYHHRG------AALMAYTNTIH 223
Cdd:PRK08974 168 vkyikrlvpkyhlpdAISFrsalhkgrrmqyvkPELVPEDLAfLQYTGGTTGVAKGAMLTHRNmlanleQAKAAYGPLLH 247
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 224 AGmakHAVYLWTLPMFHcngwcfpwtlaVQAGTHVCLRWVR----------PKPIYDAIAD---HGVTHLCGAPVVMSVL 290
Cdd:PRK08974 248 PG---KELVVTALPLYH-----------IFALTVNCLLFIElggqnllitnPRDIPGFVKElkkYPFTAITGVNTLFNAL 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 291 INARDEDKRTFAQTVTFNTAAAPPPEAVlsgmADAGFAVT--HL---YGLTETyGPAVVNEWHgewdDLAKGERSAkkar 365
Cdd:PRK08974 314 LNNEEFQELDFSSLKLSVGGGMAVQQAV----AERWVKLTgqYLlegYGLTEC-SPLVSVNPY----DLDYYSGSI---- 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 366 qGVRYAALEgLTVMDPETMQATPadGETiGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSK 445
Cdd:PRK08974 381 -GLPVPSTE-IKLVDDDGNEVPP--GEP-GELWVKGPQVMLGYWQRPEATDEVIKDGWLATGDIAVMDEEGFLRIVDRKK 455
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 446 DIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVeLKPGKTASEAEIIEHCRALLARFKVPKAVIFA- 524
Cdd:PRK08974 456 DMILVSGFNVYPNEIEDVVMLHPKVLEVAAVGVPSEVSGEAVKIFV-VKKDPSLTEEELITHCRRHLTGYKVPKLVEFRd 534
|
570
....*....|....*..
gi 499217198 525 EIPKTSTGKIQKFRLRE 541
Cdd:PRK08974 535 ELPKSNVGKILRRELRD 551
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
68-544 |
1.21e-29 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 122.68 E-value: 1.21e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 68 KGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPLVidyd 147
Cdd:PRK08751 75 KGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVVIDNFGTTVQQVIADTPVKQVI---- 150
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 148 DPDYAADAPYPKGERI-----------------GALDYEDFVAGGDEDfawSMP--DDEWDAIS-LNYTSGTTGNPKGVV 207
Cdd:PRK08751 151 TTGLGDMLGFPKAALVnfvvkyvkklvpeyrinGAIRFREALALGRKH---SMPtlQIEPDDIAfLQYTGGTTGVAKGAM 227
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 208 YHHR--------GAALMAYTNTIHAGmakHAVYLWTLPMFH-----CNGWCFpwtLAVQAGTHVCLRWVRPKPIYDAIAD 274
Cdd:PRK08751 228 LTHRnlvanmqqAHQWLAGTGKLEEG---CEVVITALPLYHifaltANGLVF---MKIGGCNHLISNPRDMPGFVKELKK 301
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 275 HGVTHLCGAPVVMSVLINARDEDKRTFAqTVTFNTAAApppEAVLSGMADAGFAVTHL-----YGLTETYGPAVVNEWhg 349
Cdd:PRK08751 302 TRFTAFTGVNTLFNGLLNTPGFDQIDFS-SLKMTLGGG---MAVQRSVAERWKQVTGLtlveaYGLTETSPAACINPL-- 375
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 350 ewdDLAKGERSAkkarqGVRYAALEGLTVMDPETMQATpadGEtIGEVMFRGNIVMKGYLKNRKASDEAF-AGGWFHSGD 428
Cdd:PRK08751 376 ---TLKEYNGSI-----GLPIPSTDACIKDDAGTVLAI---GE-IGELCIKGPQVMKGYWKRPEETAKVMdADGWLHTGD 443
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 429 LGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGE-VPVAYVELKPGKTASEAEiiEH 507
Cdd:PRK08751 444 IARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIAMMPGVLEVAAVGVPDEKSGEiVKVVIVKKDPALTAEDVK--AH 521
|
490 500 510
....*....|....*....|....*....|....*...
gi 499217198 508 CRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLREMAK 544
Cdd:PRK08751 522 ARANLTGYKQPRIIEFrKELPKTNVGKILRRELRDAAK 559
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
44-541 |
7.96e-29 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 120.26 E-value: 7.96e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 44 RISYRDFWRRSLKVASALQKR-GIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIV 122
Cdd:cd05928 41 KWSFRELGSLSRKAANVLSGAcGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVT 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 123 DREFS----GVVRQALDLAkvTPLVIDYDDpdyaadapypkgeRIGALDYEDFVAGGDEDFAwSMPDDEWDAISLNYTSG 198
Cdd:cd05928 121 SDELApevdSVASECPSLK--TKLLVSEKS-------------RDGWLNFKELLNEASTEHH-CVETGSQEPMAIYFTSG 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 199 TTGNPKgVVYHHRGAALMAYT----------------NTIHAGMAKHAvyLWTLpmFHcngwcfPWTLAVQAGTHVCLRw 262
Cdd:cd05928 185 TTGSPK-MAEHSHSSLGLGLKvngrywldltasdimwNTSDTGWIKSA--WSSL--FE------PWIQGACVFVHHLPR- 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 263 VRPKPIYDAIADHGVTHLCGAPVVMSVLINaRDEDKRTFAqtvtfntaaapppeavlsgmadagfAVTHLYGLTETYGPA 342
Cdd:cd05928 253 FDPLVILKTLSSYPITTFCGAPTVYRMLVQ-QDLSSYKFP-------------------------SLQHCVTGGEPLNPE 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 343 VVNEWhgewddlakgersakKARQGVR----YAALE-GLTVMDPETMQ--------ATPA-DGETIGEvmfRGNIVMKG- 407
Cdd:cd05928 307 VLEKW---------------KAQTGLDiyegYGQTEtGLICANFKGMKikpgsmgkASPPyDVQIIDD---NGNVLPPGt 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 408 -------------------YLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHP 468
Cdd:cd05928 369 egdigirvkpirpfglfsgYVDNPEKTAATIRGDFYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHP 448
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 499217198 469 SVASCGVVARHDDKWGEVPVAYVELKPGKTASE-----AEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLRE 541
Cdd:cd05928 449 AVVESAVVSSPDPIRGEVVKAFVVLAPQFLSHDpeqltKELQQHVKSVTAPYKYPRKVEFvQELPKTVTGKIQRNELRD 527
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
33-543 |
1.40e-28 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 120.11 E-value: 1.40e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 33 PDHIAIIH------GRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLsntpPML-EAHFGvpMTKAV----LHSLNT 101
Cdd:cd05967 65 GDQIALIYdspvtgTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYM----PMIpEAAIA--MLACArigaIHSVVF 138
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 102 RLDAAV-IAFQLDHAETKVLI---VDREFSGVV------RQALDLAKVTPLVIDYDDPDYAADAPYPKGeriGALDYEDF 171
Cdd:cd05967 139 GGFAAKeLASRIDDAKPKLIVtasCGIEPGKVVpykpllDKALELSGHKPHHVLVLNRPQVPADLTKPG---RDLDWSEL 215
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 172 VAggDEDFAWSMPDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTN--TIHaGMAKHAVYLWTLPMfhcnGWCFPWT 249
Cdd:cd05967 216 LA--KAEPVDCVPVAATDPLYILYTSGTTGKPKGVVRDNGGHAVALNWSmrNIY-GIKPGDVWWAASDV----GWVVGHS 288
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 250 LAVQA----GTHVCLRWVRPKPIYDA------IADHGVTHLCGAPVVMSVLinaRDEDkrtfaqtvtfntaaappPEAVL 319
Cdd:cd05967 289 YIVYGpllhGATTVLYEGKPVGTPDPgafwrvIEKYQVNALFTAPTAIRAI---RKED-----------------PDGKY 348
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 320 SGMADAGfAVTHLYG------------LTETYGPAVVNEWhgeW---------------DDLAKGERSAKKARQGVRyaa 372
Cdd:cd05967 349 IKKYDLS-SLRTLFLagerldpptlewAENTLGVPVIDHW---WqtetgwpitanpvglEPLPIKAGSPGKPVPGYQ--- 421
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 373 legLTVMDpETMQATPADGEtigevmfrGNIVMKGYL---------KNRKASDEAFAG---GWFHSGDLGVMHPDGYIQL 440
Cdd:cd05967 422 ---VQVLD-EDGEPVGPNEL--------GNIVIKLPLppgclltlwKNDERFKKLYLSkfpGYYDTGDAGYKDEDGYLFI 489
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 441 KDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRAL-------LA 513
Cdd:cd05967 490 MGRTDDVINVAGHRLSTGEMEESVLSHPAVAECAVVGVRDELKGQVPLGLVVLKEGVKITAEELEKELVALvreqigpVA 569
|
570 580 590
....*....|....*....|....*....|
gi 499217198 514 RFKvpKAVIFAEIPKTSTGKIQKFRLREMA 543
Cdd:cd05967 570 AFR--LVIFVKRLPKTRSGKILRRTLRKIA 597
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
25-534 |
1.80e-28 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 118.60 E-value: 1.80e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLD 104
Cdd:cd17651 1 FERQAARTPDAPALVAEGRRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYP 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 105 AAVIAFQLDHAETKVlivdrefsgVVRQALDLAKVTPLVIDYDDPDYAADAPYPKGERIGALDyedfvaggdedfawsmP 184
Cdd:cd17651 81 AERLAFMLADAGPVL---------VLTHPALAGELAVELVAVTLLDQPGAAAGADAEPDPALD----------------A 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 185 DDEWDAIslnYTSGTTGNPKGVVYHHRGAA-LMAYTNTIHAGMAKHAVYLWTLPMFHCNGW-CFPwTLAVQAGTHVCLRW 262
Cdd:cd17651 136 DDLAYVI---YTSGSTGRPKGVVMPHRSLAnLVAWQARASSLGPGARTLQFAGLGFDVSVQeIFS-TLCAGATLVLPPEE 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 263 VRPKPiyDAIAD----HGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAapppEAVLSGMADAGFAVTHLYG-LTE 337
Cdd:cd17651 212 VRTDP--PALAAwldeQRISRVFLPTVALRALAEHGRPLGVRLAALRYLLTGG----EQLVLTEDLREFCAGLPGLrLHN 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 338 TYGPA---VVNEW-----HGEWDDLAKGERSAKKARqgvryaalegLTVMDpETMQATPaDGETiGEVMFRGNIVMKGYL 409
Cdd:cd17651 286 HYGPTethVVTALslpgdPAAWPAPPPIGRPIDNTR----------VYVLD-AALRPVP-PGVP-GELYIGGAGLARGYL 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 410 KNRKASDEAFAGGWF-------HSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDK 482
Cdd:cd17651 353 NRPELTAERFVPDPFvpgarmyRTGDLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARHPGVREAVVLAREDRP 432
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 499217198 483 WGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKA-VIFAEIPKTSTGKI 534
Cdd:cd17651 433 GEKRLVAYVVGDPEAPVDAAELRAALATHLPEYMVPSAfVLLDALPLTPNGKL 485
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
195-536 |
2.12e-28 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 115.82 E-value: 2.12e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 195 YTSGTTGNPKGVVYHHRgAALMAYTNTIHAGM--AKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHV--CLRwVRPKPIYD 270
Cdd:cd17635 8 FTSGTTGEPKAVLLANK-TFFAVPDILQKEGLnwVVGDVTYLPLPATHIGGLWWILTCLIHGGLCVtgGEN-TTYKSLFK 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 271 AIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEA-VLSGMADAGFAVTHLYGLTETyGPAVVNEWHG 349
Cdd:cd17635 86 ILTTNAVTTTCLVPTLLSKLVSELKSANATVPSLRLIGYGGSRAIAAdVRFIEATGLTNTAQVYGLSET-GTALCLPTDD 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 350 EWDDLAKGERSAKKARqgVRYAALEGLTVmdpetmqatPADGEtiGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDL 429
Cdd:cd17635 165 DSIEINAVGRPYPGVD--VYLAATDGIAG---------PSASF--GTIWIKSPANMLGYWNNPERTAEVLIDGWVNTGDL 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 430 GVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAE-IIEHC 508
Cdd:cd17635 232 GERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVASAELDENAIRaLKHTI 311
|
330 340
....*....|....*....|....*....
gi 499217198 509 RALLARFKVPKAVIFAE-IPKTSTGKIQK 536
Cdd:cd17635 312 RRELEPYARPSTIVIVTdIPRTQSGKVKR 340
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
17-541 |
3.71e-28 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 118.15 E-value: 3.71e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 17 QPLTPLTYLERAAKTYPD----HIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFG---- 88
Cdd:cd05906 8 APRTLLELLLRAAERGPTkgitYIDADGSEEFQSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWAcvla 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 89 --VPMTKAVLHSlNTRLDAAVIafQLDHA----ETKVLIVDREfsgVVRQALDLAKVTPLvidyddpdyaadapypKGER 162
Cdd:cd05906 88 gfVPAPLTVPPT-YDEPNARLR--KLRHIwqllGSPVVLTDAE---LVAEFAGLETLSGL----------------PGIR 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 163 IGALDYEDfVAGGDEDFAWSMPDDewdAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCN 242
Cdd:cd05906 146 VLSIEELL-DTAADHDLPQSRPDD---LALLMLTSGSTGFPKAVPLTHRNILARSAGKIQHNGLTPQDVFLNWVPLDHVG 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 243 GWCFPWTLAVQAGT---HVCLRWV--RPKPIYDAIADHGVTHlCGAPVVMSVLIN---ARDEDKR-TFAQTVTFNTAAap 313
Cdd:cd05906 222 GLVELHLRAVYLGCqqvHVPTEEIlaDPLRWLDLIDRYRVTI-TWAPNFAFALLNdllEEIEDGTwDLSSLRYLVNAG-- 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 314 ppEAVLSGMADA-----------GFAVTHLYGLTETYGPAVVNewhgewDDLAKGERSakkarQGVRYAAL----EGLT- 377
Cdd:cd05906 299 --EAVVAKTIRRllrllepyglpPDAIRPAFGMTETCSGVIYS------RSFPTYDHS-----QALEFVSLgrpiPGVSm 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 378 -VMDPETmQATPAdgETIGEVMFRGNIVMKGYLKNRKASDEAF-AGGWFHSGDLGVMHpDGYIQLKDRSKDIIISGGENI 455
Cdd:cd05906 366 rIVDDEG-QLLPE--GEVGRLQVRGPVVTKGYYNNPEANAEAFtEDGWFRTGDLGFLD-NGNLTITGRTKDTIIVNGVNY 441
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 456 SSIEVEDALYKHPSV-----ASCGVvarHDDKWGE-------VPVAYVELKPGKTASEAEiiehcRALLARFKV-PKAVI 522
Cdd:cd05906 442 YSHEIEAAVEEVPGVepsftAAFAV---RDPGAETeelaiffVPEYDLQDALSETLRAIR-----SVVSREVGVsPAYLI 513
|
570 580
....*....|....*....|..
gi 499217198 523 ---FAEIPKTSTGKIQKFRLRE 541
Cdd:cd05906 514 plpKEEIPKTSLGKIQRSKLKA 535
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
25-544 |
5.82e-27 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 114.97 E-value: 5.82e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAII----HG--RQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTP----PMLE-AHFGVPmtk 93
Cdd:cd05966 59 LDRHLKERGDKVAIIwegdEPdqSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPelviAMLAcARIGAV--- 135
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 94 avlHS-LNTRLDAAVIAFQLDHAETKVLI-VDREFSG--------VVRQALDLAkvtPLVIDYDDPDYAADAPYPKGERi 163
Cdd:cd05966 136 ---HSvVFAGFSAESLADRINDAQCKLVItADGGYRGgkviplkeIVDEALEKC---PSVEKVLVVKRTGGEVPMTEGR- 208
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 164 gALDYEDFVAGGDEDFAWSMPDDEwDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNT-----IHAG-----MAK----- 228
Cdd:cd05966 209 -DLWWHDLMAKQSPECEPEWMDSE-DPLFILYTSGSTGKPKGVVHTTGGYLLYAATTFkyvfdYHPDdiywcTADigwit 286
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 229 -HAvYLWTLPMfhCNGWcfpwTLAVQAGThvclrwvrpkPIY-------DAIADHGVTHLCGAPVVMSVLINARDE---- 296
Cdd:cd05966 287 gHS-YIVYGPL--ANGA----TTVMFEGT----------PTYpdpgrywDIVEKHKVTIFYTAPTAIRALMKFGDEwvkk 349
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 297 -----------------------------DKR-----TFAQTVTfntaaapppeavlsgmadAGFAVTHLYGLTETyGPA 342
Cdd:cd05966 350 hdlsslrvlgsvgepinpeawmwyyevigKERcpivdTWWQTET------------------GGIMITPLPGATPL-KPG 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 343 vvnewhgewddlakgerSAKKARQGVRYAalegltVMDPETmqaTPADGETigevmfRGNIVMK----GYLKNRKASDEA 418
Cdd:cd05966 411 -----------------SATRPFFGIEPA------ILDEEG---NEVEGEV------EGYLVIKrpwpGMARTIYGDHER 458
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 419 FA-------GGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYV 491
Cdd:cd05966 459 YEdtyfskfPGYYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFV 538
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*..
gi 499217198 492 ELKPGKTAS---EAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKIQKFRLREMAK 544
Cdd:cd05966 539 TLKDGEEPSdelRKELRKHVRKEIGPIATPDKIQFVPgLPKTRSGKIMRRILRKIAA 595
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
25-533 |
1.29e-26 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 114.95 E-value: 1.29e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFG--------VPmtkavl 96
Cdd:COG1020 482 FEAQAARTPDAVAVVFGDQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAvlkagaayVP------ 555
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 97 hslntrLDAAV----IAFQLDHAETKVLIVDREfsgvVRQALDLAKVTPLvidyddpdyaadapypkgerigALDYEDfV 172
Cdd:COG1020 556 ------LDPAYpaerLAYMLEDAGARLVLTQSA----LAARLPELGVPVL----------------------ALDALA-L 602
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 173 AGGDEDFAWSMPDDEwDAISLNYTSGTTGNPKGVVYHHRGAAlmaytNTIHA-----GMAKHAVYLWTLP---------M 238
Cdd:COG1020 603 AAEPATNPPVPVTPD-DLAYVIYTSGSTGRPKGVMVEHRALV-----NLLAWmqrryGLGPGDRVLQFASlsfdasvweI 676
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 239 FhcngwcfpWTLAVQAGTHVCLRWVR--PKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFaQTVTFNTAAAPP-- 314
Cdd:COG1020 677 F--------GALLSGATLVLAPPEARrdPAALAELLARHRVTVLNLTPSLLRALLDAAPEALPSL-RLVLVGGEALPPel 747
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 315 PEAVLSGMADAgfAVTHLYGLTETygpAVVNEWHgewdDLAKGERSAK-----KARQGVRyaalegLTVMDpETMQATPa 389
Cdd:COG1020 748 VRRWRARLPGA--RLVNLYGPTET---TVDSTYY----EVTPPDADGGsvpigRPIANTR------VYVLD-AHLQPVP- 810
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 390 DGEtIGEVMFRGNIVMKGYLKNRKASDEAF-------AGG-WFHSGDLGVMHPDG---YI-----QLKdrskdiiISG-- 451
Cdd:COG1020 811 VGV-PGELYIGGAGLARGYLNRPELTAERFvadpfgfPGArLYRTGDLARWLPDGnleFLgraddQVK-------IRGfr 882
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 452 ---GenissiEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAEIPK 528
Cdd:COG1020 883 ielG------EIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLPPYMVPAAVVLLLPLP 956
|
....*
gi 499217198 529 TSTGK 533
Cdd:COG1020 957 LTGNG 961
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
17-544 |
1.70e-26 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 113.16 E-value: 1.70e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 17 QPLTPLtyLERAAKtyPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHF-----GVPM 91
Cdd:PRK10946 25 LPLTDI--LTRHAA--SDAIAVICGERQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFallklGVAP 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 92 TKAvLHSLNtRLDAAVIAFQLdhaETKVLIVDRE---FSGVVR-QALDLAKVTPLVIDyddpdyaadapypKGERIGALD 167
Cdd:PRK10946 101 VNA-LFSHQ-RSELNAYASQI---EPALLIADRQhalFSDDDFlNTLVAEHSSLRVVL-------------LLNDDGEHS 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 168 YEDFVAGGDEDF-AWSMPDDEWDAISLnyTSGTTGNPKGVVYHHRGaalmaYTNTIHA-----GMAKHAVYLWTLPMFHC 241
Cdd:PRK10946 163 LDDAINHPAEDFtATPSPADEVAFFQL--SGGSTGTPKLIPRTHND-----YYYSVRRsveicGFTPQTRYLCALPAAHN 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 242 NGWCFPWTLAV-QAGTHVCLRwVRPKPI--YDAIADHGVTHLCGAPVVMSVLINARDEDKRTfAQTVTFN---TAAAPPP 315
Cdd:PRK10946 236 YPMSSPGALGVfLAGGTVVLA-PDPSATlcFPLIEKHQVNVTALVPPAVSLWLQAIAEGGSR-AQLASLKllqVGGARLS 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 316 EAVLSGM-ADAGFAVTHLYGLTEtygpAVVNewHGEWDDlakgerSAKK--ARQG--------VRYAALEGLTVmdpetm 384
Cdd:PRK10946 314 ETLARRIpAELGCQLQQVFGMAE----GLVN--YTRLDD------SDERifTTQGrpmspddeVWVADADGNPL------ 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 385 qatpADGETiGEVMFRGNIVMKGYLK----NRKASDeafAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEV 460
Cdd:PRK10946 376 ----PQGEV-GRLMTRGPYTFRGYYKspqhNASAFD---ANGFYCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEI 447
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 461 EDALYKHPSVASCGVVARHDDKWGEVPVAYV----ELKPgktaseAEIIEHCRAL-LARFKVPKAVIF-AEIPKTSTGKI 534
Cdd:PRK10946 448 ENLLLRHPAVIHAALVSMEDELMGEKSCAFLvvkePLKA------VQLRRFLREQgIAEFKLPDRVECvDSLPLTAVGKV 521
|
570
....*....|
gi 499217198 535 QKFRLREMAK 544
Cdd:PRK10946 522 DKKQLRQWLA 531
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
46-537 |
1.82e-26 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 113.76 E-value: 1.82e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 46 SYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAhfgvpMTKAVLHSL------NTrLDAAVIAFQLDHAETKV 119
Cdd:PLN02430 78 TYKEVYEEVLQIGSALRASGAEPGSRVGIYGSNCPQWIVA-----MEACAAHSLicvplyDT-LGPGAVDYIVDHAEIDF 151
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 120 LIV-DREFSGVVRQALDLAKVTPLVIDYDDPDYAADApypKGERIGALDY--EDFVAGGDEDFAWSMPDDEWDAISLNYT 196
Cdd:PLN02430 152 VFVqDKKIKELLEPDCKSAKRLKAIVSFTSVTEEESD---KASQIGVKTYswIDFLHMGKENPSETNPPKPLDICTIMYT 228
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 197 SGTTGNPKGVVYHHRGAALMA-----YTNTIHAGMAKHAVYLWTLPMFHC-----NGWCF-------------------- 246
Cdd:PLN02430 229 SGTSGDPKGVVLTHEAVATFVrgvdlFMEQFEDKMTHDDVYLSFLPLAHIldrmiEEYFFrkgasvgyyhgdlnalrddl 308
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 247 ----PWTLA--------VQAGTHVCLRWVRP--KPIYDAIADH-------GVTHLCGAPvvMSVLINARDEDKRTFAQTV 305
Cdd:PLN02430 309 melkPTLLAgvprvferIHEGIQKALQELNPrrRLIFNALYKYklawmnrGYSHKKASP--MADFLAFRKVKAKLGGRLR 386
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 306 TFNTAAAPppeavLSGMADAGFAVTHL------YGLTETYGPAVVnewhGEWDDLAK-GERSAKKARQGVRyaaLEGLTV 378
Cdd:PLN02430 387 LLISGGAP-----LSTEIEEFLRVTSCafvvqgYGLTETLGPTTL----GFPDEMCMlGTVGAPAVYNELR---LEEVPE 454
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 379 MDPETMQATPAdgetiGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDII-ISGGENISS 457
Cdd:PLN02430 455 MGYDPLGEPPR-----GEICVRGKCLFSGYYKNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIkLSQGEYVAL 529
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 458 IEVEDALYKHPSVASCGVvarHDDKWGEVPVAYVELKPGKTASEAEIIEHCRAL--LARFKVPKAVIFAEIPKTST-GKI 534
Cdd:PLN02430 530 EYLENVYGQNPIVEDIWV---YGDSFKSMLVAVVVPNEENTNKWAKDNGFTGSFeeLCSLPELKEHILSELKSTAEkNKL 606
|
...
gi 499217198 535 QKF 537
Cdd:PLN02430 607 RGF 609
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
21-539 |
2.10e-26 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 112.78 E-value: 2.10e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 21 PLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLN 100
Cdd:PRK13383 37 PYTLLAVTAARWPGRTAIIDDDGALSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPIS 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 101 TRLDAAVIAFQLDHAETKVLIVDREFSGVVRQAldlakvtplvidyddpdyaadapypkGERIGALDYEdfVAGGDEDFA 180
Cdd:PRK13383 117 TEFRSDALAAALRAHHISTVVADNEFAERIAGA--------------------------DDAVAVIDPA--TAGAEESGG 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 181 wsMPDDEWDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTI--HAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHV 258
Cdd:PRK13383 169 --RPAVAAPGRIVLLTSGTTGKPKGVPRAPQLRSAVGVWVTIldRTRLRTGSRISVAMPMFHGLGLGMLMLTIALGGTVL 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 259 CLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDE--DKRTFAQ-TVTFNTAAAPPPEAVLSGMADAGFAVTHLYGL 335
Cdd:PRK13383 247 THRHFDAEAALAQASLHRADAFTAVPVVLARILELPPRvrARNPLPQlRVVMSSGDRLDPTLGQRFMDTYGDILYNGYGS 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 336 TETYGPAVVNEwhgewDDLAKGERSAKKARQGVRYAALEgltvmdpetMQATPADGETIGEVMFRGNIVMKGYlknRKAS 415
Cdd:PRK13383 327 TEVGIGALATP-----ADLRDAPETVGKPVAGCPVRILD---------RNNRPVGPRVTGRIFVGGELAGTRY---TDGG 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 416 DEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKP 495
Cdd:PRK13383 390 GKAVVDGMTSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFVVLHP 469
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 499217198 496 GKTASEAEIIEHCRALLARFKVPKAV-IFAEIPKTSTGKIQKFRL 539
Cdd:PRK13383 470 GSGVDAAQLRDYLKDRVSRFEQPRDInIVSSIPRNPTGKVLRKEL 514
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
43-482 |
3.41e-26 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 111.68 E-value: 3.41e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 43 QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIV 122
Cdd:cd17640 4 KRITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVALVV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 123 DrefsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvaggdedfawsmpDDEWDAISLNYTSGTTGN 202
Cdd:cd17640 84 E-------------------------------------------------------------NDSDDLATIIYTSGTTGN 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 203 PKGVVYHHRGaaLMAYTNTIHAGMAKHA--VYLWTLPMFHCNGWCFPWTLAVQAGTHVClrwVRPKPIYDAIADHGVTHL 280
Cdd:cd17640 103 PKGVMLTHAN--LLHQIRSLSDIVPPQPgdRFLSILPIWHSYERSAEYFIFACGCSQAY---TSIRTLKDDLKRVKPHYI 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 281 CGAPVVMSVLINARDEDKR--TFAQTVTFNTA-AAPPPEAVLSG---MAD--------AGFAVTHLYGLTETYGPAVVNE 346
Cdd:cd17640 178 VSVPRLWESLYSGIQKQVSksSPIKQFLFLFFlSGGIFKFGISGggaLPPhvdtffeaIGIEVLNGYGLTETSPVVSARR 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 347 WhgeWDDLAKgerSAKKARQGVRYaaleglTVMDPETMQATPADGEtiGEVMFRGNIVMKGYLKNRKASDEAF-AGGWFH 425
Cdd:cd17640 258 L---KCNVRG---SVGRPLPGTEI------KIVDPEGNVVLPPGEK--GIVWVRGPQVMKGYYKNPEATSKVLdSDGWFN 323
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*...
gi 499217198 426 SGDLGVMHPDGYIQLKDRSKD-IIISGGENISSIEVEDALYKHPSVASCGVVArHDDK 482
Cdd:cd17640 324 TGDLGWLTCGGELVLTGRAKDtIVLSNGENVEPQPIEEALMRSPFIEQIMVVG-QDQK 380
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
25-539 |
3.44e-26 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 111.26 E-value: 3.44e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLD 104
Cdd:cd12115 5 VEAQAARTPDAIALVCGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLDPAYP 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 105 AAVIAFQLDHAETKVLIVDREfsgvvrqalDLAKVTplvidyddpdyaadapypkgerigaldyedfvaggdedfawsmp 184
Cdd:cd12115 85 PERLRFILEDAQARLVLTDPD---------DLAYVI-------------------------------------------- 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 185 ddewdaislnYTSGTTGNPKGVVYHHRGA-ALMAYTNTIHAGMAKHAVYLWT-----LPMFHCNGwcfpwTLAVqAGTHV 258
Cdd:cd12115 112 ----------YTSGSTGRPKGVAIEHRNAaAFLQWAAAAFSAEELAGVLASTsicfdLSVFELFG-----PLAT-GGKVV 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 259 CLRWVRPKPIYDAIADhgVTHLCGAPVVMSVLINARDedkrtFAQTV-TFNTAAAP-PPEAVLSGMADAGFA-VTHLYGL 335
Cdd:cd12115 176 LADNVLALPDLPAAAE--VTLINTVPSAAAELLRHDA-----LPASVrVVNLAGEPlPRDLVQRLYARLQVErVVNLYGP 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 336 TE--TYGPAVVNEWHgewddlAKGERSAKKARQGVRyaalegLTVMDpETMQATPADgeTIGEVMFRGNIVMKGYLKNRK 413
Cdd:cd12115 249 SEdtTYSTVAPVPPG------ASGEVSIGRPLANTQ------AYVLD-RALQPVPLG--VPGELYIGGAGVARGYLGRPG 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 414 ASDEAFAGGWFHS-------GDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEV 486
Cdd:cd12115 314 LTAERFLPDPFGPgarlyrtGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEAALRSIPGVREAVVVAIGDAAGERR 393
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 499217198 487 PVAYVELKPGKTASEAEIIEHCRALLARFKVP-KAVIFAEIPKTSTGKIQKFRL 539
Cdd:cd12115 394 LVAYIVAEPGAAGLVEDLRRHLGTRLPAYMVPsRFVRLDALPLTPNGKIDRSAL 447
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
389-534 |
1.06e-25 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 107.49 E-value: 1.06e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 389 ADGETIGEVMFRGNIVMKGYLKNRKASdeafAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHP 468
Cdd:cd17633 179 ADGGEIGKIFVKSEMVFSGYVRGGFSN----PDGWMSVGDIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIP 254
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 499217198 469 SVASCGVVARHDDKWGEVPVAYVElkpGKTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKI 534
Cdd:cd17633 255 GIEEAIVVGIPDARFGEIAVALYS---GDKLTYKQLKRFLKQKLSRYEIPKKIIFVDsLPYTSSGKI 318
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
45-543 |
1.38e-25 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 110.11 E-value: 1.38e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 45 ISYRDFWRRSLKVASALQKRGIGkGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDR 124
Cdd:cd05909 8 LTYRKLLTGAIALARKLAKMTKE-GENVGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRELRACIKLAGIKTVLTSK 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 125 EFsgvvrqaLDLAKVTPLVIDYDDPDYA----ADAPYPKGERIGALDYEDFVAGGDEDFAWSMPDDEWDAISLNYTSGTT 200
Cdd:cd05909 87 QF-------IEKLKLHHLFDVEYDARIVyledLRAKISKADKCKAFLAGKFPPKWLLRIFGVAPVQPDDPAVILFTSGSE 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 201 GNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLRW--VRPKPIYDAIADHGVT 278
Cdd:cd05909 160 GLPKGVVLSHKNLLANVEQITAIFDPNPEDVVFGALPFFHSFGLTGCLWLPLLSGIKVVFHPnpLDYKKIPELIYDKKAT 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 279 HLCGAPVVMSVLINARDEDkrTFAqTVTFNTAAApppEAVLSGMADA-----GFAVTHLYGLTETYGPAVVNewhgewdd 353
Cdd:cd05909 240 ILLGTPTFLRGYARAAHPE--DFS-SLRLVVAGA---EKLKDTLRQEfqekfGIRILEGYGTTECSPVISVN-------- 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 354 lakgeRSAKKARQGVRYAALEGLTV--MDPETMQATPAdGETiGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGV 431
Cdd:cd05909 306 -----TPQSPNKEGTVGRPLPGMEVkiVSVETHEEVPI-GEG-GLLLVRGPNVMLGYLNEPELTSFAFGDGWYDTGDIGK 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 432 MHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKH-PSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHcrA 510
Cdd:cd05909 379 IDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSEIlPEDNEVAVVSVPDGRKGEKIVLLTTTTDTDPSSLNDILKN--A 456
|
490 500 510
....*....|....*....|....*....|....
gi 499217198 511 LLARFKVPKAVIFAE-IPKTSTGKIQKFRLREMA 543
Cdd:cd05909 457 GISNLAKPSYIHQVEeIPLLGTGKPDYVTLKALA 490
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
33-534 |
1.80e-25 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 109.09 E-value: 1.80e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 33 PDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQL 112
Cdd:cd17650 1 PDAIAVSDATRQLTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYML 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 113 DHAETKVLIVDREfsgvvrqalDLAKVTplvidyddpdyaadapypkgerigaldyedfvaggdedfawsmpddewdais 192
Cdd:cd17650 81 EDSGAKLLLTQPE---------DLAYVI---------------------------------------------------- 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 193 lnYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAG--THVCLRWVR--PKPI 268
Cdd:cd17650 100 --YTSGTTGKPKGVMVEHRNVAHAAHAWRREYELDSFPVRLLQMASFSFDVFAGDFARSLLNGgtLVICPDEVKldPAAL 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 269 YDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQ----TVTFNTAAAPPPEAVLSGMAdAGFAVTHLYGLTETygpAVV 344
Cdd:cd17650 178 YDLILKSRITLMESTPALIRPVMAYVYRNGLDLSAmrllIVGSDGCKAQDFKTLAARFG-QGMRIINSYGVTEA---TID 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 345 NEWHGEWDDLAKGERSAKKARQGVRYAalegLTVMDpETMQATPADgeTIGEVMFRGNIVMKGYLKNRKASDEAF----- 419
Cdd:cd17650 254 STYYEEGRDPLGDSANVPIGRPLPNTA----MYVLD-ERLQPQPVG--VAGELYIGGAGVARGYLNRPELTAERFvenpf 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 420 -AGG-WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVelKPGK 497
Cdd:cd17650 327 aPGErMYRTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDEAVVAVREDKGGEARLCAYV--VAAA 404
|
490 500 510
....*....|....*....|....*....|....*...
gi 499217198 498 TASEAEIIEHCRALLARFKVPKAVI-FAEIPKTSTGKI 534
Cdd:cd17650 405 TLNTAELRAFLAKELPSYMIPSYYVqLDALPLTPNGKV 442
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
33-534 |
3.52e-25 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 108.11 E-value: 3.52e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 33 PDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQL 112
Cdd:cd17652 1 PDAPAVVFGDETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYML 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 113 DHAETKVLivdrefsgvVRQALDLAKVTplvidyddpdyaadapypkgerigaldyedfvaggdedfawsmpddewdais 192
Cdd:cd17652 81 ADARPALL---------LTTPDNLAYVI---------------------------------------------------- 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 193 lnYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKHAVYL-WTLPMFHCNGWcfPWTLAVQAGTHVCL----RWVRPKP 267
Cdd:cd17652 100 --YTSGSTGRPKGVVVTHRGLANLAAAQIAAFDVGPGSRVLqFASPSFDASVW--ELLMALLAGATLVLapaeELLPGEP 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 268 IYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFaqtvtFNTAAAPPPEAVlsgmadAGFAVTHL----YGLTETygpAV 343
Cdd:cd17652 176 LADLLREHRITHVTLPPAALAALPPDDLPDLRTL-----VVAGEACPAELV------DRWAPGRRminaYGPTET---TV 241
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 344 VNEWHgewdDLAKGERSAKKAR--QGVRyaalegLTVMDpETMQATPAdGEtIGEVMFRGNIVMKGYLKNRKASDEAFAG 421
Cdd:cd17652 242 CATMA----GPLPGGGVPPIGRpvPGTR------VYVLD-ARLRPVPP-GV-PGELYIAGAGLARGYLNRPGLTAERFVA 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 422 GWF--------HSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVEL 493
Cdd:cd17652 309 DPFgapgsrmyRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPGVAEAVVVVRDDRPGDKRLVAYVVP 388
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 499217198 494 KPGKTASEAEIIEHCRALLARFKVPKA-VIFAEIPKTSTGKI 534
Cdd:cd17652 389 APGAAPTAAELRAHLAERLPGYMVPAAfVVLDALPLTPNGKL 430
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
43-541 |
7.51e-25 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 107.44 E-value: 7.51e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 43 QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIV 122
Cdd:cd05940 2 EALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLVV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 123 DREFsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvaggdedfawsmpddewdaisLNYTSGTTGN 202
Cdd:cd05940 82 DAAL------------------------------------------------------------------YIYTSGTTGL 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 203 PKGVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLR-WVRPKPIYDAIADHGVT--- 278
Cdd:cd05940 96 PKAAIISHRRAWRGGAFFAGSGGALPSDVLYTCLPLYHSTALIVGWSACLASGATLVIRkKFSASNFWDDIRKYQATifq 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 279 -------HLCGAPvvmsvlinARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADAgfAVTHLYGLTEtygpAVVNEWHGEW 351
Cdd:cd05940 176 yigelcrYLLNQP--------PKPTERKHKVRMIFGNGLRPDIWEEFKERFGVP--RIAEFYAATE----GNSGFINFFG 241
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 352 DDLAKGeRSAKKARQGVRYAALEgltvMDPETMQAT-PADG-----------ETIGEVMFRGNivMKGYLKNRKASD--- 416
Cdd:cd05940 242 KPGAIG-RNPSLLRKVAPLALVK----YDLESGEPIrDAEGrcikvprgepgLLISRINPLEP--FDGYTDPAATEKkil 314
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 417 -EAFAGG--WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGV----VARHDDKWGevpVA 489
Cdd:cd05940 315 rDVFKKGdaWFNTGDLMRLDGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVygvqVPGTDGRAG---MA 391
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 499217198 490 YVELKPGKTASEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLRE 541
Cdd:cd05940 392 AIVLQPNEEFDLSALAAHLEKNLPGYARPLFLRLQpEMEITGTFKQQKVDLRN 444
|
|
| ACSBG_like |
cd05933 |
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ... |
43-466 |
8.47e-25 |
|
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341256 [Multi-domain] Cd Length: 596 Bit Score: 108.21 E-value: 8.47e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 43 QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIV 122
Cdd:cd05933 7 HTLTYKEYYEACRQAAKAFLKLGLERFHGVGILGFNSPEWFIAAVGAIFAGGIAVGIYTTNSPEACQYVAETSEANILVV 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 123 DRefsgvvRQALD--------LAKVTPLVIDYDDPdyaadapypKGERIGALDYEDFVAGGDEDfawsmPDDEWDAI--- 191
Cdd:cd05933 87 EN------QKQLQkilqiqdkLPHLKAIIQYKEPL---------KEKEPNLYSWDEFMELGRSI-----PDEQLDAIiss 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 192 -------SLNYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMA----KHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVC- 259
Cdd:cd05933 147 qkpnqccTLIYTSGTTGMPKGVMLSHDNITWTAKAASQHMDLRpatvGQESVVSYLPLSHIAAQILDIWLPIKVGGQVYf 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 260 -------------LRWVRPkpiydaiadhgvTHLCGAPVV--------------------------MSV----------- 289
Cdd:cd05933 227 aqpdalkgtlvktLREVRP------------TAFMGVPRVwekiqekmkavgaksgtlkrkiaswaKGVgletnlklmgg 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 290 -------------LINARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADAGFAVTHLYGLTETYGPAVVNewhgewDDLAK 356
Cdd:cd05933 295 espsplfyrlakkLVFKKVRKALGLDRCQKFFTGAAPISRETLEFFLSLNIPIMELYGMSETSGPHTIS------NPQAY 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 357 GERSAKKARQGVRYaaleglTVMDPEtmqatpADGetIGEVMFRGNIVMKGYLKNRKASDEAF-AGGWFHSGDLGVMHPD 435
Cdd:cd05933 369 RLLSCGKALPGCKT------KIHNPD------ADG--IGEICFWGRHVFMGYLNMEDKTEEAIdEDGWLHSGDLGKLDED 434
|
490 500 510
....*....|....*....|....*....|..
gi 499217198 436 GYIQLKDRSKDIII-SGGENISSIEVEDALYK 466
Cdd:cd05933 435 GFLYITGRIKELIItAGGENVPPVPIEDAVKK 466
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
33-540 |
1.71e-24 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 106.30 E-value: 1.71e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 33 PDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQL 112
Cdd:cd17649 1 PDAVALVFGDQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAERLRYML 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 113 DHAETKVLIvdrefsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvAGGDEDFAWSMpddewdais 192
Cdd:cd17649 81 EDSGAGLLL---------------------------------------------------THHPRQLAYVI--------- 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 193 lnYTSGTTGNPKGVVYHHrgAALMAYTNTIHA--GMAKHAVYLWTLPM----FHcNGWCFPWTlavqAGTHVCLR----W 262
Cdd:cd17649 101 --YTSGSTGTPKGVAVSH--GPLAAHCQATAEryGLTPGDRELQFASFnfdgAH-EQLLPPLI----CGACVVLRpdelW 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 263 VRPKPIYDAIADHGVTHLCGAPVVMSVLIN-ARDEDKRTF-----------AQTVTFNTAAAPPPEAVLSGmadagfavt 330
Cdd:cd17649 172 ASADELAEMVRELGVTVLDLPPAYLQQLAEeADRTGDGRPpslrlyifggeALSPELLRRWLKAPVRLFNA--------- 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 331 hlYGLTETYGPAVVneWHGEWDDLAKGERSAKKARQGVRYAAlegltVMDPEtMQATPaDGETiGEVMFRGNIVMKGYLK 410
Cdd:cd17649 243 --YGPTEATVTPLV--WKCEAGAARAGASMPIGRPLGGRSAY-----ILDAD-LNPVP-VGVT-GELYIGGEGLARGYLG 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 411 NRKASDEAF-------AGG-WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARhDDK 482
Cdd:cd17649 311 RPELTAERFvpdpfgaPGSrLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVAL-DGA 389
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 499217198 483 WGEVPVAYVELKPGKTASE--AEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQKFRLR 540
Cdd:cd17649 390 GGKQLVAYVVLRAAAAQPElrAQLRTALRASLPDYMVPAHLVFlARLPLTPNGKLDRKALP 450
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
46-475 |
3.21e-24 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 105.04 E-value: 3.21e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 46 SYRDFWRRSLKVASALQKR-GIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDR 124
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAgGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDS 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 125 EFsgvvrqALDLAKVTPLVIDYDDPDYAADAPYPKGERigaldyeDFVAGGDEDFAWSMpddewdaislnYTSGTTGNPK 204
Cdd:TIGR01733 81 AL------ASRLAGLVLPVILLDPLELAALDDAPAPPP-------PDAPSGPDDLAYVI-----------YTSGSTGRPK 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 205 GVVYHHRGAA-LMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVclrwVRPKPIYD-------AIADHG 276
Cdd:TIGR01733 137 GVVVTHRSLVnLLAWLARRYGLDPDDRVLQFASLSFDASVEEIFGALLAGATLVV----PPEDEERDdaallaaLIAEHP 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 277 VTHLCGAPVVMSVLINARDEDKRTFaQTVTFnTAAAPPPEAVLSGMADAGFAVTH-LYGLTETygpAVVNEWHGEWDDLA 355
Cdd:TIGR01733 213 VTVLNLTPSLLALLAAALPPALASL-RLVIL-GGEALTPALVDRWRARGPGARLInLYGPTET---TVWSTATLVDPDDA 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 356 KGERSAK--KARQGVRYAalegltVMDPETMQAtpADGETiGEVMFRGNIVMKGYLKNRKASDEAFA---------GGWF 424
Cdd:TIGR01733 288 PRESPVPigRPLANTRLY------VLDDDLRPV--PVGVV-GELYIGGPGVARGYLNRPELTAERFVpdpfaggdgARLY 358
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*....
gi 499217198 425 HSGDLGVMHPDGYI--------QLKDRskdiiisgGENISSIEVEDALYKHPSVASCGV 475
Cdd:TIGR01733 359 RTGDLVRYLPDGNLeflgriddQVKIR--------GYRIELGEIEAALLRHPGVREAVV 409
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
459-533 |
3.67e-24 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 96.07 E-value: 3.67e-24
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 499217198 459 EVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFA-EIPKTSTGK 533
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGVELLEEELVAHVREELGPYAVPKEVVFVdELPKTRSGK 76
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
26-541 |
7.79e-23 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 101.64 E-value: 7.79e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 26 ERAAKTyPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDA 105
Cdd:cd17655 5 EQAEKT-PDHTAVVFEDQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPDYPE 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 106 AVIAFQLDHAETKVLIVDREFSGVVRQAldlakvtplvidyddpdyaadapypkgERIGALDYEDFVAGGDEDFAW-SMP 184
Cdd:cd17655 84 ERIQYILEDSGADILLTQSHLQPPIAFI---------------------------GLIDLLDEDTIYHEESENLEPvSKS 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 185 DDEWDAIslnYTSGTTGNPKGVVYHHRGAA--LMAYTNTIHAG----MAKHAVYLWTLP---MFHcngwcfpwtlAVQAG 255
Cdd:cd17655 137 DDLAYVI---YTSGSTGKPKGVMIEHRGVVnlVEWANKVIYQGehlrVALFASISFDASvteIFA----------SLLSG 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 256 THVCLrwVRPKPIYDA------IADHGVTHLCGAPVVMSVLinaRDEDKRTFAQTVTFNTAA-APPPEAV--LSGMADAG 326
Cdd:cd17655 204 NTLYI--VRKETVLDGqaltqyIRQNRITIIDLTPAHLKLL---DAADDSEGLSLKHLIVGGeALSTELAkkIIELFGTN 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 327 FAVTHLYGLTETYGPAVVNEWHGEWDdlAKGERSAKKARQGVRyaalegLTVMDpETMQATPAdGETiGEVMFRGNIVMK 406
Cdd:cd17655 279 PTITNAYGPTETTVDASIYQYEPETD--QQVSVPIGKPLGNTR------IYILD-QYGRPQPV-GVA-GELYIGGEGVAR 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 407 GYLKNRKASDEAF------AGG-WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARH 479
Cdd:cd17655 348 GYLNRPELTAEKFvddpfvPGErMYRTGDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEIEARLLQHPDIKEAVVIARK 427
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 499217198 480 DDKWGEVPVAYVelkpgktASEAEI-IEHCRALLAR----FKVPKAVI-FAEIPKTSTGKIQKFRLRE 541
Cdd:cd17655 428 DEQGQNYLCAYI-------VSEKELpVAQLREFLARelpdYMIPSYFIkLDEIPLTPNGKVDRKALPE 488
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
27-544 |
5.57e-22 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 100.38 E-value: 5.57e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 27 RAAKTYPDHIAIIHGRQR-ISYRDFWRRSLKVASALqKRGIGKGDTVTVML-SNTPPMLeAHFGVPMTKAVLHSLNTRLD 104
Cdd:PRK08633 623 DTAKRNWSRLAVADSTGGeLSYGKALTGALALARLL-KRELKDEENVGILLpPSVAGAL-ANLALLLAGKVPVNLNYTAS 700
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 105 AAVIAFQLDHAETKVLIVDREF------SGVVRQALDLAKVTPLvidyddpdyaadapypkgerigaldyEDFVAG-GDE 177
Cdd:PRK08633 701 EAALKSAIEQAQIKTVITSRKFleklknKGFDLELPENVKVIYL--------------------------EDLKAKiSKV 754
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 178 DFAWSM--------------------PDDEWDAIslnYTSGTTGNPKGVVYHHRGaaLMAytnTIHA-----GMAKHAVY 232
Cdd:PRK08633 755 DKLTALlaarllparllkrlygptfkPDDTATII---FSSGSEGEPKGVMLSHHN--ILS---NIEQisdvfNLRNDDVI 826
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 233 LWTLPMFHCNG-----WcFPWTLAVQAGTHvclrwvrPKP-----IYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFA 302
Cdd:PRK08633 827 LSSLPFFHSFGltvtlW-LPLLEGIKVVYH-------PDPtdalgIAKLVAKHRATILLGTPTFLRLYLRNKKLHPLMFA 898
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 303 QTVTFNTAAAPPPEAVlsgmADA---GFAVTHL--YGLTETYGPAVVNEWhgewDDLAKGERSAKKARQGVRYAALEGLT 377
Cdd:PRK08633 899 SLRLVVAGAEKLKPEV----ADAfeeKFGIRILegYGATETSPVASVNLP----DVLAADFKRQTGSKEGSVGMPLPGVA 970
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 378 V--MDPETMQATPAdGETiGEVMFRGNIVMKGYLKNRKASDEAFA----GGWFHSGDLGVMHPDGYIQLKDR----SKdI 447
Cdd:PRK08633 971 VriVDPETFEELPP-GED-GLILIGGPQVMKGYLGDPEKTAEVIKdidgIGWYVTGDKGHLDEDGFLTITDRysrfAK-I 1047
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 448 iisGGENISSIEVEDALYK--HPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEAEIIEHCRaLLARFKVPKAVIFAE 525
Cdd:PRK08633 1048 ---GGEMVPLGAVEEELAKalGGEEVVFAVTAVPDEKKGEKLVVLHTCGAEDVEELKRAIKESG-LPNLWKPSRYFKVEA 1123
|
570
....*....|....*....
gi 499217198 526 IPKTSTGKIQKFRLREMAK 544
Cdd:PRK08633 1124 LPLLGSGKLDLKGLKELAL 1142
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
33-534 |
1.22e-21 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 98.11 E-value: 1.22e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 33 PDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQL 112
Cdd:cd12114 1 PDATAVICGDGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAARREAIL 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 113 DHAETKVLIVDREFSgVVRQALDLAKVTPLVidyddpdyaadapypkgerigALDYEDFVAGGDEDfawsmPDDEWDAIs 192
Cdd:cd12114 81 ADAGARLVLTDGPDA-QLDVAVFDVLILDLD---------------------ALAAPAPPPPVDVA-----PDDLAYVI- 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 193 lnYTSGTTGNPKGVVYHHRGAAlmaytNTIHAGMAKHAV----YLWTLPMFHCNgwcfpwtLAV-------QAGTHVCL- 260
Cdd:cd12114 133 --FTSGSTGTPKGVMISHRAAL-----NTILDINRRFAVgpddRVLALSSLSFD-------LSVydifgalSAGATLVLp 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 261 -RWVRPKPIY--DAIADHGVTHLCGAPVVMSVLIN-ARDEDKRTfaqtvtfntaaaPPPEAV-LSGmaD----------- 324
Cdd:cd12114 199 dEARRRDPAHwaELIERHGVTLWNSVPALLEMLLDvLEAAQALL------------PSLRLVlLSG--Dwipldlparlr 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 325 ---AGFAVTHLYGLTETygpAVVNEWHgewdDLAKGERSAKKARQGV-----RYAalegltVMDPetMQATPADGeTIGE 396
Cdd:cd12114 265 alaPDARLISLGGATEA---SIWSIYH----PIDEVPPDWRSIPYGRplanqRYR------VLDP--RGRDCPDW-VPGE 328
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 397 VMFRGNIVMKGYLKNRKASDEAF-----AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVA 471
Cdd:cd12114 329 LWIGGRGVALGYLGDPELTAARFvthpdGERLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIELGEIEAALQAHPGVA 408
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 499217198 472 SCGVVARhDDKWGEVPVAYVELKPGKT-ASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKI 534
Cdd:cd12114 409 RAVVVVL-GDPGGKRLAAFVVPDNDGTpIAPDALRAFLAQTLPAYMIPSRVIALEaLPLTANGKV 472
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
3-539 |
2.05e-21 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 99.26 E-value: 2.05e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 3 NSYEQDLDRNAANHQPLTpltylERAAKTyPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPM 82
Cdd:PRK12316 1993 DRTPEAYPRGPGVHQRIA-----EQAARA-PEAIAVVFGDQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFEL 2066
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 83 LEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDRefsgvvrqalDLAKVTPLVidyddpdyaadapypkgER 162
Cdd:PRK12316 2067 VVALLAVLKAGGAYVPLDPNYPAERLAYMLEDSGAALLLTQR----------HLLERLPLP-----------------AG 2119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 163 IGALDYEDfvAGGDEDFAWSMPDDEWDAISLN---YTSGTTGNPKGVVYHHrgAALMAYTNTIHA--GMAKHAVYLWTLP 237
Cdd:PRK12316 2120 VARLPLDR--DAEWADYPDTAPAVQLAGENLAyviYTSGSTGLPKGVAVSH--GALVAHCQAAGEryELSPADCELQFMS 2195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 238 mFHCNGWCFPWTLAVQAGTHVCLR----WVrPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFA-QTVTFNTAAA 312
Cdd:PRK12316 2196 -FSFDGAHEQWFHPLLNGARVLIRddelWD-PEQLYDEMERHGVTILDFPPVYLQQLAEHAERDGRPPAvRVYCFGGEAV 2273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 313 PPPEAVLSGMADAGFAVTHLYGLTETYgpAVVNEWHGEWDDLAKGERSAKKARQGVRYAaleglTVMDpETMQATPADGe 392
Cdd:PRK12316 2274 PAASLRLAWEALRPVYLFNGYGPTEAV--VTPLLWKCRPQDPCGAAYVPIGRALGNRRA-----YILD-ADLNLLAPGM- 2344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 393 tIGEVMFRGNIVMKGYLKNRKASDEAF--------AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDAL 464
Cdd:PRK12316 2345 -AGELYLGGEGLARGYLNRPGLTAERFvpdpfsasGERLYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARL 2423
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 499217198 465 YKHPSVASCGVVARhDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKIQKFRL 539
Cdd:PRK12316 2424 QAHPAVREAVVVAQ-DGASGKQLVAYVVPDDAAEDLLAELRAWLAARLPAYMVPAHWVVLErLPLNPNGKLDRKAL 2498
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
7-536 |
6.86e-21 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 97.54 E-value: 6.86e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 7 QDLDRNAANHQPLTPLTYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAH 86
Cdd:PRK12467 500 VRWNAPATEYAPDCVHQLIEAQARQHPERPALVFGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGL 579
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 87 FGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREfsgvVRQALDL-AKVTPLVIDyddpdyaadapypkgeriga 165
Cdd:PRK12467 580 LAVLKAGGAYVPLDPEYPQDRLAYMLDDSGVRLLLTQSH----LLAQLPVpAGLRSLCLD-------------------- 635
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 166 lDYEDFVAGGDEDFawsmPDDEWDAISLNY---TSGTTGNPKGVVYHHRgaALMAYTNTIHAGM---AKHAVYLWTLPMF 239
Cdd:PRK12467 636 -EPADLLCGYSGHN----PEVALDPDNLAYviyTSGSTGQPKGVAISHG--ALANYVCVIAERLqlaADDSMLMVSTFAF 708
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 240 HCNGWCFPWTLAVQAGTHV----CLRwvRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPP 315
Cdd:PRK12467 709 DLGVTELFGALASGATLHLlppdCAR--DAEAFAALMADQGVTVLKIVPSHLQALLQASRVALPRPQRALVCGGEALQVD 786
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 316 EAVLSGMADAGFAVTHLYGLTETygPAVVNEWhgewdDLAKGERSAKKARQGVRYAALeGLTVMDPetmQATPADGETIG 395
Cdd:PRK12467 787 LLARVRALGPGARLINHYGPTET--TVGVSTY-----ELSDEERDFGNVPIGQPLANL-GLYILDH---YLNPVPVGVVG 855
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 396 EVMFRGNIVMKGYLKNRKASDEAF-------AGG-WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKH 467
Cdd:PRK12467 856 ELYIGGAGLARGYHRRPALTAERFvpdpfgaDGGrLYRTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQ 935
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 499217198 468 PSVASCGVVARHDDKwGEVPVAYVELKPG-----KTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKIQK 536
Cdd:PRK12467 936 PGVREAVVLAQPGDA-GLQLVAYLVPAAVadgaeHQATRDELKAQLRQVLPDYMVPAHLLLLDsLPLTPNGKLDR 1009
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
33-539 |
7.55e-21 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 95.16 E-value: 7.55e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 33 PDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDT-VTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQ 111
Cdd:cd17648 1 PDRVAVVYGDKRLTYRELNERANRLAHYLLSVAEIRPDDlVGLVLDKSELMIIAILAVWKAGAAYVPIDPSYPDERIQFI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 112 LDHAETKVLIVDREfsgvvrqalDLAKVTplvidyddpdyaadapypkgerigaldyedfvaggdedfawsmpddewdai 191
Cdd:cd17648 81 LEDTGARVVITNST---------DLAYAI--------------------------------------------------- 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 192 slnYTSGTTGNPKGVVYHHRGA-------------------ALMAYTNTIHagmaKHAVYLWTLPMFHCNgwcfpwTLAV 252
Cdd:cd17648 101 ---YTSGTTGKPKGVLVEHGSVvnlrtslseryfgrdngdeAVLFFSNYVF----DFFVEQMTLALLNGQ------KLVV 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 253 QAGTHVclrwVRPKPIYDAIADHGVTHLCGAPVVMSVLinarDEDKRTFAQTVTfnTAAAPPPEAVLSGMAdAGFA--VT 330
Cdd:cd17648 168 PPDEMR----FDPDRFYAYINREKVTYLSGTPSVLQQY----DLARLPHLKRVD--AAGEEFTAPVFEKLR-SRFAglII 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 331 HLYGLTETYGPAVVNEWHGewddlakGERSAKKARQGVRYAALeglTVMDPEtMQATPADGetIGEVMFRGNIVMKGYLk 410
Cdd:cd17648 237 NAYGPTETTVTNHKRFFPG-------DQRFDKSLGRPVRNTKC---YVLNDA-MKRVPVGA--VGELYLGGDGVARGYL- 302
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 411 NRK------------ASDEAFAGG----WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCG 474
Cdd:cd17648 303 NRPeltaerflpnpfQTEQERARGrnarLYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECA 382
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 499217198 475 VVARHDDKWGEVP-----VAYVELKPGkTASEAEIIEHCRALLARFKVPKAVI-FAEIPKTSTGKIQKFRL 539
Cdd:cd17648 383 VVAKEDASQAQSRiqkylVGYYLPEPG-HVPESDLLSFLRAKLPRYMVPARLVrLEGIPVTINGKLDVRAL 452
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
29-518 |
8.24e-21 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 95.32 E-value: 8.24e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 29 AKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVI 108
Cdd:PRK09029 13 AQVRPQAIALRLNDEVLTWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNPQLPQPLL 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 109 AFQLDHAETKVLIVDREFsgvvRQALDLAKVTPLVIDYddpdyaadapypkgerigaldyedfvaggdedfawsMPDDEW 188
Cdd:PRK09029 93 EELLPSLTLDFALVLEGE----NTFSALTSLHLQLVEG------------------------------------AHAVAW 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 DAI---SLNYTSGTTGNPKGVVYHHRG--------AALMAYTntihagmaKHAVYLWTLPMFHCNG----WcfPWtLAVQ 253
Cdd:PRK09029 133 QPQrlaTMTLTSGSTGLPKAAVHTAQAhlasaegvLSLMPFT--------AQDSWLLSLPLFHVSGqgivW--RW-LYAG 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 254 AGTHVclrwvRPK-PIYDAIAdhGVTHlcgAPVVMSVLinardedKRTFAQTVTFNT-------AAAPPPEavLSGMADA 325
Cdd:PRK09029 202 ATLVV-----RDKqPLEQALA--GCTH---ASLVPTQL-------WRLLDNRSEPLSlkavllgGAAIPVE--LTEQAEQ 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 326 GFAVTHL-YGLTEtygpavvnewhgewddLAkgerS---AKKA--RQGVRYAaLEGLTVMDPEtmqatpadgetiGEVMF 399
Cdd:PRK09029 263 QGIRCWCgYGLTE----------------MA----StvcAKRAdgLAGVGSP-LPGREVKLVD------------GEIWL 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 400 RGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHpDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARH 479
Cdd:PRK09029 310 RGASLALGYWRQGQLVPLVNDEGWFATRDRGEWQ-NGELTILGRLDNLFFSGGEGIQPEEIERVINQHPLVQQVFVVPVA 388
|
490 500 510
....*....|....*....|....*....|....*....
gi 499217198 480 DDKWGEVPVAYVELKPGKTasEAEIIEHCRALLARFKVP 518
Cdd:PRK09029 389 DAEFGQRPVAVVESDSEAA--VVNLAEWLQDKLARFQQP 425
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
195-529 |
1.32e-20 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 94.97 E-value: 1.32e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 195 YTSGTTGNPKGVVYHHRG--AALMAYTNTIHAGMAKHAVYLWTLPMFH-----CNGWCFPW------------TLAVQAG 255
Cdd:cd17639 95 YTSGSTGNPKGVMLTHGNlvAGIAGLGDRVPELLGPDDRYLAYLPLAHifelaAENVCLYRggtigygsprtlTDKSKRG 174
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 256 THVCLRWVRPkpiydaiadhgvTHLCGAPVVMSVL---INARDEDKRTFAQTVtFNTAAAPPPEAVLSGMaDAGF----- 327
Cdd:cd17639 175 CKGDLTEFKP------------TLMVGVPAIWDTIrkgVLAKLNPMGGLKRTL-FWTAYQSKLKALKEGP-GTPLldelv 240
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 328 -----------------------AVTHL------------YGLTETYGPAVVNEWHgewdDLAKGersakkaRQGVRYAA 372
Cdd:cd17639 241 fkkvraalggrlrymlsggaplsADTQEflnivlcpviqgYGLTETCAGGTVQDPG----DLETG-------RVGPPLPC 309
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 373 LEGLTVMDPETMQATPADgETIGEVMFRGNIVMKGYLKNRKASDEAFAG-GWFHSGDLGVMHPDGYIQLKDRSKDII-IS 450
Cdd:cd17639 310 CEIKLVDWEEGGYSTDKP-PPRGEILIRGPNVFKGYYKNPEKTKEAFDGdGWFHTGDIGEFHPDGTLKIIDRKKDLVkLQ 388
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 451 GGENISSIEVEDALYKHPSVASCGVVA-----------------------RHDDKWGEVPvAYVELKPGKTASEAEIIEH 507
Cdd:cd17639 389 NGEYIALEKLESIYRSNPLVNNICVYAdpdksypvaivvpnekhltklaeKHGVINSEWE-ELCEDKKLQKAVLKSLAET 467
|
410 420
....*....|....*....|...
gi 499217198 508 CRAL-LARFKVPKAVIFAEIPKT 529
Cdd:cd17639 468 ARAAgLEKFEIPQGVVLLDEEWT 490
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
45-541 |
1.71e-20 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 94.97 E-value: 1.71e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 45 ISYRDFWRRSLKVASALQKRGI--GKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIV 122
Cdd:cd05927 6 ISYKEVAERADNIGSALRSLGGkpAPASFVGIYSINRPEWIISELACYAYSLVTVPLYDTLGPEAIEYILNHAEISIVFC 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 123 DrefsgvvrqaldlakvtplvidyddpdyaadapypKGERIgaLDYEDFVAGGDEDFAWSMPDDEWDAISLNYTSGTTGN 202
Cdd:cd05927 86 D-----------------------------------AGVKV--YSLEEFEKLGKKNKVPPPPPKPEDLATICYTSGTTGN 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 203 PKGVVYHHR-----GAALMAYTNtIHAGMAKHAVYLWTLPMFHCNGWCFPWtLAVQAGTHVCLRWVRPKPIYDAIADHGV 277
Cdd:cd05927 129 PKGVMLTHGnivsnVAGVFKILE-ILNKINPTDVYISYLPLAHIFERVVEA-LFLYHGAKIGFYSGDIRLLLDDIKALKP 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 278 THLCGAPVVMSVL---INARDEDKRTFAQTVtFNTA-----------------------------------------AAP 313
Cdd:cd05927 207 TVFPGVPRVLNRIydkIFNKVQAKGPLKRKL-FNFAlnyklaelrsgvvraspfwdklvfnkikqalggnvrlmltgSAP 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 314 PPEAVLSGM-ADAGFAVTHLYGLTETYGPAVVNeWHGEWDdlaKGERSAKKARQGVRyaaLEGLtvmdPEtMQATPADGE 392
Cdd:cd05927 286 LSPEVLEFLrVALGCPVLEGYGQTECTAGATLT-LPGDTS---VGHVGGPLPCAEVK---LVDV----PE-MNYDAKDPN 353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 393 TIGEVMFRGNIVMKGYLKNRKASDEAFA-GGWFHSGDLGVMHPDGYIQLKDRSKDII-ISGGENISSIEVEDALYKHPSV 470
Cdd:cd05927 354 PRGEVCIRGPNVFSGYYKDPEKTAEALDeDGWLHTGDIGEWLPNGTLKIIDRKKNIFkLSQGEYVAPEKIENIYARSPFV 433
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 471 ASCGVvarHDDKWGEVPVAYV-----------ELKPGKTASEAEIIEH---------------CRALLARFKVPKAVIFA 524
Cdd:cd05927 434 AQIFV---YGDSLKSFLVAIVvpdpdvlkewaASKGGGTGSFEELCKNpevkkailedlvrlgKENGLKGFEQVKAIHLE 510
|
570 580
....*....|....*....|....
gi 499217198 525 EIP-------KTSTGKIQKFRLRE 541
Cdd:cd05927 511 PEPfsvenglLTPTFKLKRPQLKK 534
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
23-515 |
3.52e-20 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 93.81 E-value: 3.52e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 23 TYLERAAKTYPDHIAII--HGR--------QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLsntPPMLE------AH 86
Cdd:PRK09274 10 RHLPRAAQERPDQLAVAvpGGRgadgklayDELSFAELDARSDAIAHGLNAAGIGRGMRAVLMV---TPSLEffaltfAL 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 87 FG---VPmtkaVLhslntrLDAAV----IAFQLDHAETKVLIvdrefsG-----VVRQALDLAK--VTPLVIDyddpdya 152
Cdd:PRK09274 87 FKagaVP----VL------VDPGMgiknLKQCLAEAQPDAFI------GipkahLARRLFGWGKpsVRRLVTV------- 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 153 adapypkGER--IGALDYEDF-VAGGDEDF--AWSMPDDEwDAISlnYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMA 227
Cdd:PRK09274 144 -------GGRllWGGTTLATLlRDGAAAPFpmADLAPDDM-AAIL--FTSGSTGTPKGVVYTHGMFEAQIEALREDYGIE 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 228 KHAVYLWTLPMFHCNGWCFPWTLAV------QAGThvclrwVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTF 301
Cdd:PRK09274 214 PGEIDLPTFPLFALFGPALGMTSVIpdmdptRPAT------VDPAKLFAAIERYGVTNLFGSPALLERLGRYGEANGIKL 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 302 A--QTVTfnTAAAPPPEAVL---SGMADAGFAVTHLYGLTETYGPAVVnewhgEWDDL--AKGERSAK-------KARQG 367
Cdd:PRK09274 288 PslRRVI--SAGAPVPIAVIerfRAMLPPDAEILTPYGATEALPISSI-----ESREIlfATRAATDNgagicvgRPVDG 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 368 VRYAALEgltVMD---PETMQATP-ADGEtIGEVMFRGNIVMKGYLKNRKASDEA----FAGGWFH-SGDLGVMHPDGYI 438
Cdd:PRK09274 361 VEVRIIA---ISDapiPEWDDALRlATGE-IGEIVVAGPMVTRSYYNRPEATRLAkipdGQGDVWHrMGDLGYLDAQGRL 436
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 499217198 439 QLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVArHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARF 515
Cdd:PRK09274 437 WFCGRKAHRVETAGGTLYTIPCERIFNTHPGVKRSALVG-VGVPGAQRPVLCVELEPGVACSKSALYQELRALAAAH 512
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
26-536 |
2.90e-19 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 92.33 E-value: 2.90e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 26 ERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDA 105
Cdd:PRK12316 518 EEQVERTPEAPALAFGEETLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPA 597
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 106 AVIAFQLDHAETKVLIVDREfsgvvrqaldLAKVTPLvidyddpdyaadapyPKGERIGALDYEDFVAGGDEDFAwsmPD 185
Cdd:PRK12316 598 ERLAYMLEDSGVQLLLSQSH----------LGRKLPL---------------AAGVQVLDLDRPAAWLEGYSEEN---PG 649
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 186 DEWDAISLN---YTSGTTGNPKGVVYHHRG-AALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLR 261
Cdd:PRK12316 650 TELNPENLAyviYTSGSTGKPKGAGNRHRAlSNRLCWMQQAYGLGVGDTVLQKTPFSFDVSVWEFFWPLMSGARLVVAAP 729
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 262 WVR--PKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPppeAVLSGMADAGFAVTHLYGLtetY 339
Cdd:PRK12316 730 GDHrdPAKLVELINREGVDTLHFVPSMLQAFLQDEDVASCTSLRRIVCSGEALP---ADAQEQVFAKLPQAGLYNL---Y 803
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 340 GP--AVVNEWHGEWDDLAKGERSAKKARQGVRYAALEGltvmdpetmQATPADGETIGEVMFRGNIVMKGYLKNRKASDE 417
Cdd:PRK12316 804 GPteAAIDVTHWTCVEEGGDSVPIGRPIANLACYILDA---------NLEPVPVGVLGELYLAGRGLARGYHGRPGLTAE 874
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 418 AFAGGWF-------HSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHddkwGEVPVAY 490
Cdd:PRK12316 875 RFVPSPFvagermyRTGDLARYRADGVIEYAGRIDHQVKLRGLRIELGEIEARLLEHPWVREAAVLAVD----GKQLVGY 950
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 499217198 491 VELKPGKTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKIQK 536
Cdd:PRK12316 951 VVLESEGGDWREALKAHLAASLPEYMVPAQWLALErLPLTPNGKLDR 997
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
47-542 |
3.22e-19 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 91.22 E-value: 3.22e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 47 YRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFG------VPMTKAVLHSLNTRlDAAV--IAFQLDHAETK 118
Cdd:PRK09192 52 YQTLRARAEAGARRLLALGLKPGDRVALIAETDGDFVEAFFAcqyaglVPVPLPLPMGFGGR-ESYIaqLRGMLASAQPA 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 119 VLIVDREFSGVVRQAldlAKVTPLVIdyddpdyaadapypkgerigALDYEDFVAGGDEDFAwsMPDDEWDAIS-LNYTS 197
Cdd:PRK09192 131 AIITPDELLPWVNEA---THGNPLLH--------------------VLSHAWFKALPEADVA--LPRPTPDDIAyLQYSS 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 198 GTTGNPKGVVYHHRgaALMAYTNTI-HAGM----AKHAVYlWtLPMFHCNGW--CFPWTLAVQA---------------- 254
Cdd:PRK09192 186 GSTRFPRGVIITHR--ALMANLRAIsHDGLkvrpGDRCVS-W-LPFYHDMGLvgFLLTPVATQLsvdylptrdfarrplq 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 255 ------------------GTHVCLRWVRPKPIYD------AIADHGvthlcGAPVVMSVLinardedkRTFAQTvtFNTA 310
Cdd:PRK09192 262 wldlisrnrgtisysppfGYELCARRVNSKDLAEldlscwRVAGIG-----ADMIRPDVL--------HQFAEA--FAPA 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 311 AAPPPEAVLS-GMADAGFAVT---HLYGL-TETygpavVNEWHGEWDDLAK--GERSAKkARQGVRYA-ALEG--LTVMD 380
Cdd:PRK09192 327 GFDDKAFMPSyGLAEATLAVSfspLGSGIvVEE-----VDRDRLEYQGKAVapGAETRR-VRTFVNCGkALPGheIEIRN 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 381 pETMQATPADGetIGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHpDGYIQLKDRSKDIIISGGENISSIEV 460
Cdd:PRK09192 401 -EAGMPLPERV--VGHICVRGPSLMSGYFRDEESQDVLAADGWLDTGDLGYLL-DGYLYITGRAKDLIIINGRNIWPQDI 476
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 461 EDALYKHPSVASCGVVA-RHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALL-ARFKVPKAVIFA---EIPKTSTGKIQ 535
Cdd:PRK09192 477 EWIAEQEPELRSGDAAAfSIAQENGEKIVLLVQCRISDEERRGQLIHALAALVrSEFGVEAAVELVpphSLPRTSSGKLS 556
|
....*..
gi 499217198 536 KFRLREM 542
Cdd:PRK09192 557 RAKAKKR 563
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
25-536 |
6.13e-19 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 91.38 E-value: 6.13e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLD 104
Cdd:PRK12467 3101 IEAQVARTPEAPALVFGDQQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYVPLDPEYP 3180
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 105 AAVIAFQLDHAETKVLIVDrefSGVVRQALDLAKVTplvidyddpdyaadapypkgerigALDYEDFVAGGDEDfawSMP 184
Cdd:PRK12467 3181 RERLAYMIEDSGVKLLLTQ---AHLLEQLPAPAGDT------------------------ALTLDRLDLNGYSE---NNP 3230
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 185 DDEWDAISLNY---TSGTTGNPKGVVYHHrgAALMAYTNTIHA--GMAKHAVYLWTLPM-FHCNGWCFPWTLAVQAGTHV 258
Cdd:PRK12467 3231 STRVMGENLAYviyTSGSTGKPKGVGVRH--GALANHLCWIAEayELDANDRVLLFMSFsFDGAQERFLWTLICGGCLVV 3308
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 259 CLRWVR-PKPIYDAIADHGVTHLCGAPVVMSVLinARDEDKRTFA--QTVTFNTAAAPPP--EAVLSGMADAGfaVTHLY 333
Cdd:PRK12467 3309 RDNDLWdPEELWQAIHAHRISIACFPPAYLQQF--AEDAGGADCAslDIYVFGGEAVPPAafEQVKRKLKPRG--LTNGY 3384
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 334 GLTEtygpAVVNEWHgeWDDLAKGERSAKKARQGVRYAALeGLTVMDPetmQATPADGETIGEVMFRGNIVMKGYLKNRK 413
Cdd:PRK12467 3385 GPTE----AVVTVTL--WKCGGDAVCEAPYAPIGRPVAGR-SIYVLDG---QLNPVPVGVAGELYIGGVGLARGYHQRPS 3454
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 414 ASDEAF-------AGG-WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARhDDKWGE 485
Cdd:PRK12467 3455 LTAERFvadpfsgSGGrLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLLQHPSVREAVVLAR-DGAGGK 3533
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 499217198 486 VPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQK 536
Cdd:PRK12467 3534 QLVAYVVPADPQGDWRETLRDHLAASLPDYMVPAQLLVlAAMPLGPNGKVDR 3585
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
40-536 |
2.48e-18 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 88.13 E-value: 2.48e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 40 HGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKA---VLHSLNTRLDAAVIAfqldhAE 116
Cdd:PRK07768 25 DAPVRHTWGEVHERARRIAGGLAAAGVGPGDAVAVLAGAPVEIAPTAQGLWMRGAsltMLHQPTPRTDLAVWA-----ED 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 117 TKVLIvdrefsgvvrQALDlakvTPLVIDYDDPDYAADAPYPKGERIGALDyeDFVAGGDEDfawsMPDDEWDAISL-NY 195
Cdd:PRK07768 100 TLRVI----------GMIG----AKAVVVGEPFLAAAPVLEEKGIRVLTVA--DLLAADPID----PVETGEDDLALmQL 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 196 TSGTTGNPKGVVYHHRG--AALMAYTNTIHAGMAKHAVYLWtLPMFHCNGWCFPWTLAVQAGTHVCLrwVRP-----KPI 268
Cdd:PRK07768 160 TSGSTGSPKAVQITHGNlyANAEAMFVAAEFDVETDVMVSW-LPLFHDMGMVGFLTVPMYFGAELVK--VTPmdflrDPL 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 269 YDA--IADHGVTHLCG---APVVMSVLINARDEDKR----------TFAQTV------TFNTAAAP---PPEAVLS--GM 322
Cdd:PRK07768 237 LWAelISKYRGTMTAApnfAYALLARRLRRQAKPGAfdlsslrfalNGAEPIdpadveDLLDAGARfglRPEAILPayGM 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 323 ADAGFAVThlygLTETYGPAVVNEWHGewDDLAKGERSAKKARQGVRYAALEGLTVMDPEtMQATPADGET-----IGEV 397
Cdd:PRK07768 317 AEATLAVS----FSPCGAGLVVDEVDA--DLLAALRRAVPATKGNTRRLATLGPPLPGLE-VRVVDEDGQVlpprgVGVI 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 398 MFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVA 477
Cdd:PRK07768 390 ELRGESVTPGYLTMDGFIPAQDADGWLDTGDLGYLTEEGEVVVCGRVKDVIIMAGRNIYPTDIERAAARVEGVRPGNAVA 469
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 499217198 478 -RHD-DKWGEVPVAYVELKPGKTASEAEIIEH--CRALLARFKV-PKAVIFAE---IPKTSTGKIQK 536
Cdd:PRK07768 470 vRLDaGHSREGFAVAVESNAFEDPAEVRRIRHqvAHEVVAEVGVrPRNVVVLGpgsIPKTPSGKLRR 536
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
13-539 |
2.67e-18 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 88.14 E-value: 2.67e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 13 AANHQPLTPLTYLERAAKTYPDHIAI--IHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVP 90
Cdd:PRK05857 8 AMPQLPSTVLDRVFEQARQQPEAIALrrCDGTSALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACA 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 91 MTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREfSGVVRQALDLAKVTPLVIDYDDPDyaadapypkgerigalDYED 170
Cdd:PRK05857 88 KLGAIAVMADGNLPIAAIERFCQITDPAAALVAPG-SKMASSAVPEALHSIPVIAVDIAA----------------VTRE 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 171 FVAGGDEDFAWSMPDDEWD-AISLNYTSGTTGNPKGVVYHHRgaALMAYTNTIHAG-------MAKHAVYLwTLPMFHCN 242
Cdd:PRK05857 151 SEHSLDAASLAGNADQGSEdPLAMIFTSGTTGEPKAVLLANR--TFFAVPDILQKEglnwvtwVVGETTYS-PLPATHIG 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 243 G-WcfpWTLAVQAGTHVCLRW-VRPKPIYDAIADHGVTHLCGAPVVMSVLINardedKRTFAQTVTfntaaaPPPEAVLS 320
Cdd:PRK05857 228 GlW---WILTCLMHGGLCVTGgENTTSLLEILTTNAVATTCLVPTLLSKLVS-----ELKSANATV------PSLRLVGY 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 321 G-----MAD------AGFAVTHLYGLTETYGPAVVnewhgewddLAKGERSAKKARQGVRYAALEGLTVM-------DPE 382
Cdd:PRK05857 294 GgsraiAADvrfieaTGVRTAQVYGLSETGCTALC---------LPTDDGSIVKIEAGAVGRPYPGVDVYlaatdgiGPT 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 383 TMQATPAdgETIGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVED 462
Cdd:PRK05857 365 APGAGPS--ASFGTLWIKSPANMLGYWNNPERTAEVLIDGWVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVDR 442
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 463 ALYKHPSVASCGVVARHDDKWGE-VPVAYVelkPGKTASEAEIIEHCRALLARF--------KVPKAVIFAEIPKTSTGK 533
Cdd:PRK05857 443 IAEGVSGVREAACYEIPDEEFGAlVGLAVV---ASAELDESAARALKHTIAARFrresepmaRPSTIVIVTDIPRTQSGK 519
|
....*.
gi 499217198 534 IQKFRL 539
Cdd:PRK05857 520 VMRASL 525
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
196-542 |
2.73e-18 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 86.64 E-value: 2.73e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 196 TSGTTGNPKGVVYhhRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCngwcfpwtlavqAGTHVCLRWV----RPKpIYDA 271
Cdd:PRK07824 43 TSGTTGTPKGAML--TAAALTASADATHDRLGGPGQWLLALPAHHI------------AGLQVLVRSViagsEPV-ELDV 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 272 IADHGVTHLCGAPVVMSV-----------LINARDEDKRTFA----QTVTFNTAAAPPPeaVLSGMADAGFAVTHLYGLT 336
Cdd:PRK07824 108 SAGFDPTALPRAVAELGGgrrytslvpmqLAKALDDPAATAAlaelDAVLVGGGPAPAP--VLDAAAAAGINVVRTYGMS 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 337 ETYGPAVVNewhgewddlakgersakkarqGVryaALEGLTVMDPEtmqatpadgetiGEVMFRGNIVMKGYlKNrKASD 416
Cdd:PRK07824 186 ETSGGCVYD---------------------GV---PLDGVRVRVED------------GRIALGGPTLAKGY-RN-PVDP 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 417 EAFAG-GWFHSGDLGVMHpDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKP 495
Cdd:PRK07824 228 DPFAEpGWFRTDDLGALD-DGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQRVVAAVVGDG 306
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 499217198 496 GKTASEAEIIEHCRALLARFKVPKAV-IFAEIPKTSTGKIQKFRLREM 542
Cdd:PRK07824 307 GPAPTLEALRAHVARTLDRTAAPRELhVVDELPRRGIGKVDRRALVRR 354
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
26-536 |
7.29e-18 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 88.09 E-value: 7.29e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 26 ERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDA 105
Cdd:PRK12316 3064 EEQVERTPDAVALAFGEQRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPE 3143
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 106 AVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVTPlvidyddpdyaadapypkgerigaldyedfvagGDEDFAWSMPD 185
Cdd:PRK12316 3144 ERLAYMLEDSGAQLLLSQSHLRLPLAQGVQVLDLDR---------------------------------GDENYAEANPA 3190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 186 DEWDAISLNY---TSGTTGNPKGVVYHHrgAALMAYTNTIHAGMAKHA---VYLWTLPMFHCNGWCFPWTLAVqaGTHVC 259
Cdd:PRK12316 3191 IRTMPENLAYviyTSGSTGKPKGVGIRH--SALSNHLCWMQQAYGLGVgdrVLQFTTFSFDVFVEELFWPLMS--GARVV 3266
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 260 LR----WVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPeavLSGMADAGFAVTHLYGL 335
Cdd:PRK12316 3267 LAgpedWRDPALLVELINSEGVDVLHAYPSMLQAFLEEEDAHRCTSLKRIVCGGEALPAD---LQQQVFAGLPLYNLYGP 3343
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 336 TETygpavvNEWHGEWDDLAKGERSAKKARQGVRYAALEGLTVMDPETMQAtpadgetIGEVMFRGNIVMKGYLKNRKAS 415
Cdd:PRK12316 3344 TEA------TITVTHWQCVEEGKDAVPIGRPIANRACYILDGSLEPVPVGA-------LGELYLGGEGLARGYHNRPGLT 3410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 416 DEAFAGGWF-------HSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHddkwGEVPV 488
Cdd:PRK12316 3411 AERFVPDPFvpgerlyRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWVREAVVLAVD----GRQLV 3486
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 499217198 489 AYVELKPGKTASEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQK 536
Cdd:PRK12316 3487 AYVVPEDEAGDLREALKAHLKASLPEYMVPAHLLFlERMPLTPNGKLDR 3535
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
26-541 |
1.05e-17 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 85.44 E-value: 1.05e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 26 ERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDA 105
Cdd:cd17653 4 ERIAAAHPDAVAVESLGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAKLPS 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 106 AVIAFQLDHAETKVLIVDRefsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvagGDEDFAWSMpd 185
Cdd:cd17653 84 ARIQAILRTSGATLLLTTD--------------------------------------------------SPDDLAYII-- 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 186 dewdaislnYTSGTTGNPKGVVYHHRGaaLMAYTNTIHAGM-----AKHAVYLwtLPMFHCNGWCFPWTLAvqAGTHVCL 260
Cdd:cd17653 112 ---------FTSGSTGIPKGVMVPHRG--VLNYVSQPPARLdvgpgSRVAQVL--SIAFDACIGEIFSTLC--NGGTLVL 176
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 261 RwVRPKPIYDAIADHGVTHLcgAPVVMSVLinardeDKRTFAQTVTFNTAAAPPPEAVLSGMAdAGFAVTHLYGLTETYG 340
Cdd:cd17653 177 A-DPSDPFAHVARTVDALMS--TPSILSTL------SPQDFPNLKTIFLGGEAVPPSLLDRWS-PGRRLYNAYGPTECTI 246
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 341 PAVVNEwhgewddLAKGER-SAKKARQGVryaaleGLTVMDPETMQatPADGETiGEVMFRGNIVMKGYLKNRKASDEAF 419
Cdd:cd17653 247 SSTMTE-------LLPGQPvTIGKPIPNS------TCYILDADLQP--VPEGVV-GEICISGVQVARGYLGNPALTASKF 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 420 AGGWFH-------SGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDkwgeVPVAYVe 492
Cdd:cd17653 311 VPDPFWpgsrmyrTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIEEVVLQSQPEVTQAAAIVVNG----RLVAFV- 385
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 499217198 493 lKPgKTASEAEIIEHCRALLARFKVPKAVI-FAEIPKTSTGKIQKFRLRE 541
Cdd:cd17653 386 -TP-ETVDVDGLRSELAKHLPSYAVPDRIIaLDSFPLTANGKVDRKALRE 433
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
43-515 |
1.77e-17 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 85.20 E-value: 1.77e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 43 QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIv 122
Cdd:cd05910 1 SRLSFRELDERSDRIAQGLTAYGIRRGMRAVLMVPPGPDFFALTFALFKAGAVPVLIDPGMGRKNLKQCLQEAEPDAFI- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 123 drefsGVVRqaldlakvtplvidyddpdyaadapypkgerigaldyedfvagGDEDFAwsmpddewdaisLNYTSGTTGN 202
Cdd:cd05910 80 -----GIPK-------------------------------------------ADEPAA------------ILFTSGSTGT 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 203 PKGVVYHHRgaalmAYTNTIHA-----GMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLRWVRPKPIYDAIADHGV 277
Cdd:cd05910 100 PKGVVYRHG-----TFAAQIDAlrqlyGIRPGEVDLATFPLFALFGPALGLTSVIPDMDPTRPARADPQKLVGAIRQYGV 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 278 THLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEAV---LSGMADAGFAVTHLYGLTETYGPAVVNewhgewDDL 354
Cdd:cd05910 175 SIVFGSPALLERVARYCAQHGITLPSLRRVLSAGAPVPIALaarLRKMLSDEAEILTPYGATEALPVSSIG------SRE 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 355 AKGERSAKKAR----------QGVRYAALEGLTVMDPETMQATPADGETIGEVMFRGNIVMKGYLKNRKA-----SDEAF 419
Cdd:cd05910 249 LLATTTAATSGgagtcvgrpiPGVRVRIIEIDDEPIAEWDDTLELPRGEIGEITVTGPTVTPTYVNRPVAtalakIDDNS 328
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 420 AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARhDDKWGEVPVAYVELKPGKTA 499
Cdd:cd05910 329 EGFWHRMGDLGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERVFNTHPGVRRSALVGV-GKPGCQLPVLCVEPLPGTIT 407
|
490
....*....|....*.
gi 499217198 500 SEAEIIEHCRALLARF 515
Cdd:cd05910 408 PRARLEQELRALAKDY 423
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
19-475 |
2.75e-17 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 85.28 E-value: 2.75e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 19 LTPLTYLERAAKTYPDHIAIihGRQRIS-----------YRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAhF 87
Cdd:PLN02861 43 DSPWQFFSDAVKKYPNNQML--GRRQVTdskvgpyvwltYKEVYDAAIRIGSAIRSRGVNPGDRCGIYGSNCPEWIIA-M 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 88 GVPMTKAVLH-SLNTRLDAAVIAFQLDHAETKVLIV-DREFSGVVRQaldLAKVTPLVIDYDDPDYAADAPYPKGERIGA 165
Cdd:PLN02861 120 EACNSQGITYvPLYDTLGANAVEFIINHAEVSIAFVqESKISSILSC---LPKCSSNLKTIVSFGDVSSEQKEEAEELGV 196
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 166 --LDYEDFVAGGDEDFawSMPDDEWDAI-SLNYTSGTTGNPKGVVYHHRgaALMAYTNTIHAGM-------AKHAVYLWT 235
Cdd:PLN02861 197 scFSWEEFSLMGSLDC--ELPPKQKTDIcTIMYTSGTTGEPKGVILTNR--AIIAEVLSTDHLLkvtdrvaTEEDSYFSY 272
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 236 LPMFHCNGWCFPwTLAVQAGTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVL---INA------------------- 293
Cdd:PLN02861 273 LPLAHVYDQVIE-TYCISKGASIGFWQGDIRYLMEDVQALKPTIFCGVPRVYDRIytgIMQkissggmlrkklfdfayny 351
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 294 ---------RDEDKRTFAQTVTFN--------------TAAAPPPEAVLSGMADAGFAV-THLYGLTETYG---PAVVNE 346
Cdd:PLN02861 352 klgnlrkglKQEEASPRLDRLVFDkikeglggrvrlllSGAAPLPRHVEEFLRVTSCSVlSQGYGLTESCGgcfTSIANV 431
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 347 WhgewddlakgersAKKARQGVRYAALEGLTVMDPEtMQATPADGETIGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHS 426
Cdd:PLN02861 432 F-------------SMVGTVGVPMTTIEARLESVPE-MGYDALSDVPRGEICLRGNTLFSGYHKRQDLTEEVLIDGWFHT 497
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 499217198 427 GDLGVMHPDGYIQLKDRSKDII-ISGGENISSIEVEDALYKHPSVASCGV 475
Cdd:PLN02861 498 GDIGEWQPNGAMKIIDRKKNIFkLSQGEYVAVENLENTYSRCPLIASIWV 547
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
53-476 |
3.11e-17 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 84.44 E-value: 3.11e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 53 RSLKVASALQKRGIGKGDTVTVMLSNTPP--------MLEAHFGVPmtkavlhsLNTRLDAAVIAFQLDHAETKVLIVdr 124
Cdd:cd05932 15 KARRLAAALRALGLEPGSKIALISKNCAEwfitdlaiWMAGHISVP--------LYPTLNPDTIRYVLEHSESKALFV-- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 125 efsGVVRQALDLAKVTPLVIDYDDPDYAADAPYPKGerigaldYEDFVAGGDEDFAWSMPDDEwDAISLNYTSGTTGNPK 204
Cdd:cd05932 85 ---GKLDDWKAMAPGVPEGLISISLPPPSAANCQYQ-------WDDLIAQHPPLEERPTRFPE-QLATLIYTSGTTGQPK 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 205 GVVYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVC-----------LRWVRP-------- 265
Cdd:cd05932 154 GVMLTFGSFAWAAQAGIEHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLVAfaesldtfvedVQRARPtlffsvpr 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 266 ------KPIYDAIADHGVTHLCGAPVVMSVLinardedKRTFAQTVTFNTA------AAPPPEAVLSGMADAGFAVTHLY 333
Cdd:cd05932 234 lwtkfqQGVQDKIPQQKLNLLLKIPVVNSLV-------KRKVLKGLGLDQCrlagcgSAPVPPALLEWYRSLGLNILEAY 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 334 GLTETYGPAVVNEwhgewddlaKGERsakkaRQGVRYAALEGLTVMDPETmqatpadgetiGEVMFRGNIVMKGYLKNRK 413
Cdd:cd05932 307 GMTENFAYSHLNY---------PGRD-----KIGTVGNAGPGVEVRISED-----------GEILVRSPALMMGYYKDPE 361
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 499217198 414 ASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDII-ISGGENISSIEVEDALYKHPSVASCGVV 476
Cdd:cd05932 362 ATAEAFtADGFLRTGDKGELDADGNLTITGRVKDIFkTSKGKYVAPAPIENKLAEHDRVEMVCVI 426
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
393-543 |
3.65e-17 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 83.89 E-value: 3.65e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 393 TIGEVMFRGNIVMKGYLKNRKASDeafagGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVAS 472
Cdd:PRK07445 300 QTGNITIQAQSLALGYYPQILDSQ-----GIFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILATGLVQD 374
|
90 100 110 120 130 140 150
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 499217198 473 CGVVARHDDKWGEVPVAYVELKPGKTaSEAEIIEHCRALLARFKVPKAVI-FAEIPKTSTGKIQKFRLREMA 543
Cdd:PRK07445 375 VCVLGLPDPHWGEVVTAIYVPKDPSI-SLEELKTAIKDQLSPFKQPKHWIpVPQLPRNPQGKINRQQLQQIA 445
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
26-536 |
8.76e-17 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 84.44 E-value: 8.76e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 26 ERAAKTyPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDA 105
Cdd:PRK12467 1582 DQAAAT-PEAVALVFGEQELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPR 1660
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 106 AVIAFQLDHAETKVLIVDREfsgvvrqaldLAKVTPLvidyddpdyaadapyPKGERIGALDYE-DFVAGGDEDFAWSMP 184
Cdd:PRK12467 1661 ERLAYMIEDSGIELLLTQSH----------LQARLPL---------------PDGLRSLVLDQEdDWLEGYSDSNPAVNL 1715
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 185 DDEWDAISLnYTSGTTGNPKGV-VYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGWCFPWTLAvqAGTHVCLR-- 261
Cdd:PRK12467 1716 APQNLAYVI-YTSGSTGRPKGAgNRHGALVNRLCATQEAYQLSAADVVLQFTSFAFDVSVWELFWPLI--NGARLVIApp 1792
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 262 --WVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAAAPPPEA---VLSGMADAGfaVTHLYGLT 336
Cdd:PRK12467 1793 gaHRDPEQLIQLIERQQVTTLHFVPSMLQQLLQMDEQVEHPLSLRRVVCGGEALEVEAlrpWLERLPDTG--LFNLYGPT 1870
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 337 ETygpAV-VNEWHGEWDDLAKGERSAKkarqGVRYAALeGLTVMDPetmQATPADGETIGEVMFRGNIVMKGYLKNRKAS 415
Cdd:PRK12467 1871 ET---AVdVTHWTCRRKDLEGRDSVPI----GQPIANL-STYILDA---SLNPVPIGVAGELYLGGVGLARGYLNRPALT 1939
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 416 DEAF--------AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVArHDDKWGEVP 487
Cdd:PRK12467 1940 AERFvadpfgtvGSRLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGVREAVVIA-QDGANGKQL 2018
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 499217198 488 VAYV--------ELKPGKTASEAEIIEHCRALLARFKVPKAVIF-AEIPKTSTGKIQK 536
Cdd:PRK12467 2019 VAYVvptdpglvDDDEAQVALRAILKNHLKASLPEYMVPAHLVFlARMPLTPNGKLDR 2076
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
419-544 |
1.41e-16 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 82.88 E-value: 1.41e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 419 FAGGWFhSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKT 498
Cdd:PRK00174 481 FKGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDDIKGQGIYAFVTLKGGEE 559
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|
gi 499217198 499 ASEA---EIIEHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLREMAK 544
Cdd:PRK00174 560 PSDElrkELRNWVRKEIGPIAKPDVIQFApGLPKTRSGKIMRRILRKIAE 609
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
29-534 |
1.71e-15 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 79.17 E-value: 1.71e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 29 AKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVpmTKA-------VLHSLNT 101
Cdd:PRK04813 12 AQTQPDFPAYDYLGEKLTYGQLKEDSDALAAFIDSLKLPDKSPIIVFGHMSPEMLATFLGA--VKAghayipvDVSSPAE 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 102 RLD--------AAVIA---FQLDHAETKVLIVDrefsgvvrqalDLAkvtplvidyddpdyaadapypkgerigaldyED 170
Cdd:PRK04813 90 RIEmiievakpSLIIAteeLPLEILGIPVITLD-----------ELK-------------------------------DI 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 171 FVAGGDEDFAWSMPDDEwdaislNY----TSGTTGNPKGVVYHHrgAALMAYTNTI--------HAGMAKHAVYLWTLPM 238
Cdd:PRK04813 128 FATGNPYDFDHAVKGDD------NYyiifTSGTTGKPKGVQISH--DNLVSFTNWMledfalpeGPQFLNQAPYSFDLSV 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 239 FHcngWcFPwTLAVqAGTHVCL-RWV--RPKPIYDAIADHGVTHLCGAP--VVMSVLINARDEDK----RTF---AQTVT 306
Cdd:PRK04813 200 MD---L-YP-TLAS-GGTLVALpKDMtaNFKQLFETLPQLPINVWVSTPsfADMCLLDPSFNEEHlpnlTHFlfcGEELP 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 307 FNTAaapppEAVLSGMADAgfavtHLYgltETYGP-----AV----VNEwhgewddlakgERSAKKARQGVRYAALEGLT 377
Cdd:PRK04813 274 HKTA-----KKLLERFPSA-----TIY---NTYGPteatvAVtsieITD-----------EMLDQYKRLPIGYAKPDSPL 329
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 378 VMDPETMQATPADGEtiGEVMFRGNIVMKGYLKNRKASDEAF---AGGW-FHSGDLGVMhPDGYIQLKDRSKDIIISGGE 453
Cdd:PRK04813 330 LIIDEEGTKLPDGEQ--GEIVISGPSVSKGYLNNPEKTAEAFftfDGQPaYHTGDAGYL-EDGLLFYQGRIDFQIKLNGY 406
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 454 NISSIEVEDALYKHPSVASCGVVARHDDkwGEVP--VAYVELKPGKTASEAEIIEHCRALLARF----KVPKAVIFAE-I 526
Cdd:PRK04813 407 RIELEEIEQNLRQSSYVESAVVVPYNKD--HKVQylIAYVVPKEEDFEREFELTKAIKKELKERlmeyMIPRKFIYRDsL 484
|
....*...
gi 499217198 527 PKTSTGKI 534
Cdd:PRK04813 485 PLTPNGKI 492
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
43-503 |
1.97e-15 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 79.00 E-value: 1.97e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 43 QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVlhSLNTRLDA--AVIAFQLDHAETKVL 120
Cdd:cd17641 10 QEFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGAL--SLGIYQDSmaEEVAYLLNYTGARVV 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 121 IV-DREfsgVVRQALDLAKVTPLVidyDDPDYAADAPYPKGERIGALDYEDFVAGG-----------DEDFAWSMPDDEw 188
Cdd:cd17641 88 IAeDEE---QVDKLLEIADRIPSV---RYVIYCDPRGMRKYDDPRLISFEDVVALGraldrrdpglyEREVAAGKGEDV- 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 dAIsLNYTSGTTGNPKGVVYHHRgaalmaytNTIHAGMAKHAV--------YLWTLPMfhcnGWCFPWTLAVQAG--THV 258
Cdd:cd17641 161 -AV-LCTTSGTTGKPKLAMLSHG--------NFLGHCAAYLAAdplgpgdeYVSVLPL----PWIGEQMYSVGQAlvCGF 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 259 CLRWVR-PKPIYDAIADHGVTHLCGAPVV---MSVLINARDEDKRTFAQTV--------------TFNTAAAPPPEAVLS 320
Cdd:cd17641 227 IVNFPEePETMMEDLREIGPTFVLLPPRVwegIAADVRARMMDATPFKRFMfelgmklglraldrGKRGRPVSLWLRLAS 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 321 GMADA----------GFA-VTHLYGLTETYGPAVVNEWHGEWDDLakgersakkaRQGVRYAALEGLTV------MDPET 383
Cdd:cd17641 307 WLADAllfrplrdrlGFSrLRSAATGGAALGPDTFRFFHAIGVPL----------KQLYGQTELAGAYTvhrdgdVDPDT 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 384 MqATPADGETI-----GEVMFRGNIVMKGYLKNRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDI-IISGGENIS 456
Cdd:cd17641 377 V-GVPFPGTEVridevGEILVRSPGVFVGYYKNPEATAEDFdEDGWLHTGDAGYFKENGHLVVIDRAKDVgTTSDGTRFS 455
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 499217198 457 SIEVEDALYKHPSVAScGVVARHDDKWgevPVAYVELKPGKTASEAE 503
Cdd:cd17641 456 PQFIENKLKFSPYIAE-AVVLGAGRPY---LTAFICIDYAIVGKWAE 498
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
7-536 |
2.08e-15 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 80.00 E-value: 2.08e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 7 QDLDRNAANHqPLTPL--TYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLE 84
Cdd:PRK12316 4538 ALWNRTDAGY-PATRCvhQLVAERARMTPDAVAVVFDEEKLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMV 4616
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 85 AHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREFSgvvrQALDLAK-VTPLVIdyddpdyaadapypkgERI 163
Cdd:PRK12316 4617 GLLAVLKAGGAYVPLDPEYPRERLAYMMEDSGAALLLTQSHLL----QRLPIPDgLASLAL----------------DRD 4676
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 164 GalDYEDFvagGDEDfawsmPDDEWDAISLNY---TSGTTGNPKGVVYHHRG-AALMAYTNTIHAGMAKHAVYLWTLPMF 239
Cdd:PRK12316 4677 E--DWEGF---PAHD-----PAVRLHPDNLAYviyTSGSTGRPKGVAVSHGSlVNHLHATGERYELTPDDRVLQFMSFSF 4746
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 240 HCNGWCFPWTLAvqAGTHVCLR----WVrPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVTFNTAaappp 315
Cdd:PRK12316 4747 DGSHEGLYHPLI--NGASVVIRddslWD-PERLYAEIHEHRVTVLVFPPVYLQQLAEHAERDGEPPSLRVYCFGG----- 4818
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 316 EAVLSGMADAGFAVTHLYGLTETYGPAVVNEWHGEWDDLAKGERSAKKARQGVRYAALEGLtVMDpetMQATPADGETIG 395
Cdd:PRK12316 4819 EAVAQASYDLAWRALKPVYLFNGYGPTETTVTVLLWKARDGDACGAAYMPIGTPLGNRSGY-VLD---GQLNPLPVGVAG 4894
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 396 EVMFRGNIVMKGYLKNRKASDEAF--------AGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKH 467
Cdd:PRK12316 4895 ELYLGGEGVARGYLERPALTAERFvpdpfgapGGRLYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIEARLREH 4974
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 499217198 468 PSVASCGVVARhDDKWGEVPVAYV-----ELKPGkTASEAEIIEHCRALLAR----FKVPKAVIF-AEIPKTSTGKIQK 536
Cdd:PRK12316 4975 PAVREAVVIAQ-EGAVGKQLVGYVvpqdpALADA-DEAQAELRDELKAALRErlpeYMVPAHLVFlARMPLTPNGKLDR 5051
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
43-541 |
7.80e-15 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 77.33 E-value: 7.80e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 43 QRISYRDFWRRSLKVASALQK-RGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLI 121
Cdd:cd05938 4 ETYTYRDVDRRSNQAARALLAhAGLRPGDTVALLLGNEPAFLWIWLGLAKLGCPVAFLNTNIRSKSLLHCFRCCGAKVLV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 122 VDREFSGVVRQALDLAKvtplvidyddpdyaadapyPKGERIGALDYEDFVAG-------GDEDFAWSMPDDEWDAISLN 194
Cdd:cd05938 84 VAPELQEAVEEVLPALR-------------------ADGVSVWYLSHTSNTEGvislldkVDAASDEPVPASLRAHVTIK 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 195 ------YTSGTTGNPK-GVVYHHRGAALMAYTNTIHAGmAKHAVYLwTLPMFHCNGWCFPWTLAVQAGTHVCLRwvrPK- 266
Cdd:cd05938 145 spalyiYTSGTTGLPKaARISHLRVLQCSGFLSLCGVT-ADDVIYI-TLPLYHSSGFLLGIGGCIELGATCVLK---PKf 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 267 ---PIYDAIADHGVT----------HLCGAPvvmsvlinARDEDKrtfAQTVTFntaaapppeAVLSGM-AD-------- 324
Cdd:cd05938 220 sasQFWDDCRKHNVTviqyigellrYLCNQP--------QSPNDR---DHKVRL---------AIGNGLrADvwreflrr 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 325 -AGFAVTHLYGLTETyGPAVVNeWHGEWDDLAKGERSAKKArqgVRYAalegLTVMDPETmqATP---ADGETI----GE 396
Cdd:cd05938 280 fGPIRIREFYGSTEG-NIGFFN-YTGKIGAVGRVSYLYKLL---FPFE----LIKFDVEK--EEPvrdAQGFCIpvakGE 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 397 VmfrGNIVMK--------GYLKNRKASD-----EAFAGG--WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVE 461
Cdd:cd05938 349 P---GLLVAKitqqspflGYAGDKEQTEkkllrDVFKKGdvYFNTGDLLVQDQQNFLYFHDRVGDTFRWKGENVATTEVA 425
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 462 DALYKHPSVASCGV----VARHDdkwGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAV-IFAEIPKTSTGKIQK 536
Cdd:cd05938 426 DVLGLLDFLQEVNVygvtVPGHE---GRIGMAAVKLKPGHEFDGKKLYQHVREYLPAYARPRFLrIQDSLEITGTFKQQK 502
|
....*
gi 499217198 537 FRLRE 541
Cdd:cd05938 503 VRLVE 507
|
|
| PLN02614 |
PLN02614 |
long-chain acyl-CoA synthetase |
28-462 |
1.40e-14 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 166255 [Multi-domain] Cd Length: 666 Bit Score: 76.60 E-value: 1.40e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 28 AAKTYPDHIAIihGRQRIS-----------YRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLeahfgVPMTKAVL 96
Cdd:PLN02614 54 SVEKYPNNPML--GRREIVdgkpgkyvwqtYQEVYDIVIKLGNSLRSVGVKDEAKCGIYGANSPEWI-----ISMEACNA 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 97 HSLNT-----RLDAAVIAFQLDHAETKVLIVD-REFSGVVRQALDLAKVTPLVIDYDDPDYAADApypKGERIGALDY-- 168
Cdd:PLN02614 127 HGLYCvplydTLGAGAVEFIISHSEVSIVFVEeKKISELFKTCPNSTEYMKTVVSFGGVSREQKE---EAETFGLVIYaw 203
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 169 EDFVAGGdEDFAWSMP-DDEWDAISLNYTSGTTGNPKGVVYHHRG-----AALMAYTNTIHAGMAKHAVYLWTLPMFHCN 242
Cdd:PLN02614 204 DEFLKLG-EGKQYDLPiKKKSDICTIMYTSGTTGDPKGVMISNESivtliAGVIRLLKSANAALTVKDVYLSYLPLAHIF 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 243 GW----CFpwtlaVQAGTHVCLRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDED--------KRTFAQTVTF--- 307
Cdd:PLN02614 283 DRvieeCF-----IQHGAAIGFWRGDVKLLIEDLGELKPTIFCAVPRVLDRVYSGLQKKlsdggflkKFVFDSAFSYkfg 357
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 308 -------NTAAAPPPEA----------------VLSGMAD------------AGFAVTHLYGLTETYGPAVVNeWHGEWD 352
Cdd:PLN02614 358 nmkkgqsHVEASPLCDKlvfnkvkqglggnvriILSGAAPlashvesflrvvACCHVLQGYGLTESCAGTFVS-LPDELD 436
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 353 DLakGERSAKKARQGVRyaaLEGLTVMDPETMQATPAdgetiGEVMFRGNIVMKGYLKNRKASDEAFAGGWFHSGDLGVM 432
Cdd:PLN02614 437 ML--GTVGPPVPNVDIR---LESVPEMEYDALASTPR-----GEICIRGKTLFSGYYKREDLTKEVLIDGWLHTGDVGEW 506
|
490 500 510
....*....|....*....|....*....|.
gi 499217198 433 HPDGYIQLKDRSKDII-ISGGENISSIEVED 462
Cdd:PLN02614 507 QPNGSMKIIDRKKNIFkLSQGEYVAVENIEN 537
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
2-534 |
2.18e-14 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 76.62 E-value: 2.18e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 2 ANSYEQDLDRNAANHQ-PLTPLTYL--ERAAKTyPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSN 78
Cdd:PRK10252 439 PGEYAQLAQVNATAVEiPETTLSALvaQQAAKT-PDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSVAVALPR 517
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 79 TPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDREFSGvvRQAlDLAKVTPLVIDYDDPdyaadapyp 158
Cdd:PRK10252 518 SVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTADQLP--RFA-DVPDLTSLCYNAPLA--------- 585
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 159 kgerigaldyedfvAGGDEDFAWSMPDDEWDAIslnYTSGTTGNPKGVVYHHRgaalmAYTNTI-----HAGMAKHAVYL 233
Cdd:PRK10252 586 --------------PQGAAPLQLSQPHHTAYII---FTSGSTGRPKGVMVGQT-----AIVNRLlwmqnHYPLTADDVVL 643
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 234 WTLPM-FHCNGWCFPWTLAVQAgthvCLRWVRPK----PIY--DAIADHGVTHLCGAPVVMSVLINARD-EDKRTFAQTV 305
Cdd:PRK10252 644 QKTPCsFDVSVWEFFWPFIAGA----KLVMAEPEahrdPLAmqQFFAEYGVTTTHFVPSMLAAFVASLTpEGARQSCASL 719
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 306 T--FNTAAAPPPEAVLSGMADAGFAVTHLYGLTETygpAV-VNEWHGEWDDLAKgersakkarqgVRYAAL--------E 374
Cdd:PRK10252 720 RqvFCSGEALPADLCREWQQLTGAPLHNLYGPTEA---AVdVSWYPAFGEELAA-----------VRGSSVpigypvwnT 785
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 375 GLTVMDpETMQATPADgeTIGEVMFRGNIVMKGYLKNRKASDEAFAGGWF-------HSGDLGVMHPDGYIQLKDRSKDI 447
Cdd:PRK10252 786 GLRILD-ARMRPVPPG--VAGDLYLTGIQLAQGYLGRPDLTASRFIADPFapgermyRTGDVARWLDDGAVEYLGRSDDQ 862
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 448 IISGGENISSIEVEDALYKHPSVASCGVVAR----HDDKWGEVP--VAYVELKPGKTASEAEIIEHCRALLARFKVPKAV 521
Cdd:PRK10252 863 LKIRGQRIELGEIDRAMQALPDVEQAVTHACvinqAAATGGDARqlVGYLVSQSGLPLDTSALQAQLRERLPPHMVPVVL 942
|
570
....*....|....
gi 499217198 522 I-FAEIPKTSTGKI 534
Cdd:PRK10252 943 LqLDQLPLSANGKL 956
|
|
| PRK05851 |
PRK05851 |
long-chain-fatty acid--ACP ligase MbtM; |
184-542 |
4.14e-14 |
|
long-chain-fatty acid--ACP ligase MbtM;
Pssm-ID: 180289 [Multi-domain] Cd Length: 525 Bit Score: 74.80 E-value: 4.14e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 184 PDDEWDAIsLNYTSGTTGNPKGVVYHhRGAALMAYTNTI-HAGM-AKHAVYLWTLPMFHCNGWCFPWTLAVqAGTHVclr 261
Cdd:PRK05851 149 PDSGGPAV-LQGTAGSTGTPRTAILS-PGAVLSNLRGLNaRVGLdAATDVGCSWLPLYHDMGLAFLLTAAL-AGAPL--- 222
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 262 WVRPKPIYDA--------IADHGVThLCGAPVVMSVLIN---ARDEDKRTFAQTVTFNtaAAPPP-----EAVLSGMADA 325
Cdd:PRK05851 223 WLAPTTAFSAspfrwlswLSDSRAT-LTAAPNFAYNLIGkyaRRVSDVDLGALRVALN--GGEPVdcdgfERFATAMAPF 299
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 326 GF---AVTHLYGLTETyGPAVVNEWHGE---WDDLAKGERSAKKaRQGVRYAALEGLTVMDPETMQATPADGETIGEVMF 399
Cdd:PRK05851 300 GFdagAAAPSYGLAES-TCAVTVPVPGIglrVDEVTTDDGSGAR-RHAVLGNPIPGMEVRISPGDGAAGVAGREIGEIEI 377
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 400 RGNIVMKGYLknrkASDEAFAGGWFHSGDLGVMHPDGYIqLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARH 479
Cdd:PRK05851 378 RGASMMSGYL----GQAPIDPDDWFPTGDLGYLVDGGLV-VCGRAKELITVAGRNIFPTEIERVAAQVRGVREGAVVAVG 452
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 499217198 480 DDKWGEVP--VAYVELK-PGKTASEAEIIEHCRALLArfKVPKAVIFA---EIPKTSTGKIQKFRLREM 542
Cdd:PRK05851 453 TGEGSARPglVIAAEFRgPDEAGARSEVVQRVASECG--VVPSDVVFVapgSLPRTSSGKLRRLAVKRS 519
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
23-543 |
7.82e-14 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 73.73 E-value: 7.82e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 23 TYLERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPP----MLeahfgvpmtkAVLhs 98
Cdd:cd05918 3 DLIEERARSQPDAPAVCAWDGSLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWavvaML----------AVL-- 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 99 lntRLDAAVIAfqLDHAetkvLIVDRefsgvVRQALDLAKvTPLVIDyddpdyaadapypkgerigaldyedfvaggded 178
Cdd:cd05918 71 ---KAGGAFVP--LDPS----HPLQR-----LQEILQDTG-AKVVLT--------------------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 179 fawSMPDDewDAISLnYTSGTTGNPKGVVYHHRGAALMAYTNTIHAGMAKH------AVYLWTLPMFHCngwcFpWTLAv 252
Cdd:cd05918 103 ---SSPSD--AAYVI-FTSGSTGKPKGVVIEHRALSTSALAHGRALGLTSEsrvlqfASYTFDVSILEI----F-TTLA- 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 253 qAGTHVCL--RWVRPKPIYDAIADHGVTHLCGAPVVMSVLinardedkrtfaqtvtfntaaapPPEAV--LSGMADAGFA 328
Cdd:cd05918 171 -AGGCLCIpsEEDRLNDLAGFINRLRVTWAFLTPSVARLL-----------------------DPEDVpsLRTLVLGGEA 226
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 329 VT-----------HLYGLtetYGPAvvnewhgEWDDLAKGERSAKKARQGVRYAALEGLT-VMDPETMQATPADGEtIGE 396
Cdd:cd05918 227 LTqsdvdtwadrvRLINA---YGPA-------ECTIAATVSPVVPSTDPRNIGRPLGATCwVVDPDNHDRLVPIGA-VGE 295
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 397 VMFRGNIVMKGYLKNRKASDEAF---------AGGWFHS-----GDLGVMHPDGYIQLKDRsKD--IIISG-----Geni 455
Cdd:cd05918 296 LLIEGPILARGYLNDPEKTAAAFiedpawlkqEGSGRGRrlyrtGDLVRYNPDGSLEYVGR-KDtqVKIRGqrvelG--- 371
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 456 ssiEVEDALYKHPSVAS---CGVVARHDDKWGEVPVAYVELKPGKTASE-----------------AEIIEHCRALLARF 515
Cdd:cd05918 372 ---EIEHHLRQSLPGAKevvVEVVKPKDGSSSPQLVAFVVLDGSSSGSGdgdslflepsdefralvAELRSKLRQRLPSY 448
|
570 580
....*....|....*....|....*....
gi 499217198 516 KVPKAVI-FAEIPKTSTGKIQKFRLREMA 543
Cdd:cd05918 449 MVPSVFLpLSHLPLTASGKIDRRALRELA 477
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
44-543 |
8.72e-14 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 74.16 E-value: 8.72e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 44 RISYRDFWRRSLKVASALQKRGIGKGDTVTVMLsntPPMLE---AHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVL 120
Cdd:PLN02654 120 SLTYSELLDRVCQLANYLKDVGVKKGDAVVIYL---PMLMElpiAMLACARIGAVHSVVFAGFSAESLAQRIVDCKPKVV 196
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 121 IVdrefSGVVRQALDLAKVTPLVidyddpDYAADAPYPKGERIGA-LDYEDFVAGGDEDFAWSMPDDEW----------- 188
Cdd:PLN02654 197 IT----CNAVKRGPKTINLKDIV------DAALDESAKNGVSVGIcLTYENQLAMKREDTKWQEGRDVWwqdvvpnyptk 266
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 ---------DAISLNYTSGTTGNPKGVVyhHRGAALMAYTNTIHagmaKHAVYLWTLPMFHCNGWCfPW----------- 248
Cdd:PLN02654 267 cevewvdaeDPLFLLYTSGSTGKPKGVL--HTTGGYMVYTATTF----KYAFDYKPTDVYWCTADC-GWitghsyvtygp 339
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 249 -----TLAVQAGTHvclRWVRPKPIYDAIADHGVTHLCGAPVVMSVLINARDE--------------------------- 296
Cdd:PLN02654 340 mlngaTVLVFEGAP---NYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDEyvtrhsrkslrvlgsvgepinpsawrw 416
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 297 ------DKR-----TFAQTVTFNTAAAPPPEAVLSGMADAGFAvthLYGLTetygPAVVNEWHGEWDDLAKGERSAKKAR 365
Cdd:PLN02654 417 ffnvvgDSRcpisdTWWQTETGGFMITPLPGAWPQKPGSATFP---FFGVQ----PVIVDEKGKEIEGECSGYLCVKKSW 489
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 366 QGVrYAALEGltvmDPETMQATpadgetigevmfrgnivmkgYLKnrkasdeAFAGGWFhSGDLGVMHPDGYIQLKDRSK 445
Cdd:PLN02654 490 PGA-FRTLYG----DHERYETT--------------------YFK-------PFAGYYF-SGDGCSRDKDGYYWLTGRVD 536
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 446 DIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASE---AEIIEHCRALLARFKVPKAVI 522
Cdd:PLN02654 537 DVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVEGVPYSEelrKSLILTVRNQIGAFAAPDKIH 616
|
570 580
....*....|....*....|..
gi 499217198 523 FAE-IPKTSTGKIQKFRLREMA 543
Cdd:PLN02654 617 WAPgLPKTRSGKIMRRILRKIA 638
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
425-543 |
2.18e-13 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 71.99 E-value: 2.18e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 425 HSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGE-VPVAYVElkpGKTASEAE 503
Cdd:PRK08308 294 FTKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGErVKAKVIS---HEEIDPVQ 370
|
90 100 110 120
....*....|....*....|....*....|....*....|.
gi 499217198 504 IIEHCRALLARFKVP-KAVIFAEIPKTSTGKIQKfRLREMA 543
Cdd:PRK08308 371 LREWCIQHLAPYQVPhEIESVTEIPKNANGKVSR-KLLELG 410
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
46-541 |
4.15e-13 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 71.31 E-value: 4.15e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 46 SYRDFWRRSLKVASALQK-RGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDr 124
Cdd:cd05937 7 TYSETYDLVLRYAHWLHDdLGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAAPAFINYNLSGDPLIHCLKLSGSRFVIVD- 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 125 efsgvvrqaldlakvtplvidyddpdyaadapypkgerigaldyedfvaggDEDFAwsmpddewdaiSLNYTSGTTGNPK 204
Cdd:cd05937 86 ---------------------------------------------------PDDPA-----------ILIYTSGTTGLPK 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 205 GVVYHHR----GAALMAYTntihAGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCL-RWVRPKPIYDAIADHGVTH 279
Cdd:cd05937 104 AAAISWRrtlvTSNLLSHD----LNLKNGDRTYTCMPLYHGTAAFLGACNCLMSGGTLALsRKFSASQFWKDVRDSGATI 179
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 280 LCGAPVVMSVLINARDE--DKRTFAQTVTFNTAAappPEavLSGMADAGFAVT---HLYGLTETYGpAVVNEWHGEWDDL 354
Cdd:cd05937 180 IQYVGELCRYLLSTPPSpyDRDHKVRVAWGNGLR---PD--IWERFRERFNVPeigEFYAATEGVF-ALTNHNVGDFGAG 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 355 AKGER---SAKKARQG---VRYAALEGLTVMDPETMQATPADGETIGEVMFRGNIVMK----GYLKNRKASD-----EAF 419
Cdd:cd05937 254 AIGHHgliRRWKFENQvvlVKMDPETDDPIRDPKTGFCVRAPVGEPGEMLGRVPFKNReafqGYLHNEDATEsklvrDVF 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 420 AGG--WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGV----VARHDdkwGEVPVAYVEL 493
Cdd:cd05937 334 RKGdiYFRTGDLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVLGAHPDIAEANVygvkVPGHD---GRAGCAAITL 410
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 499217198 494 KPGKTASEAEII----EHCRALLARFKVPKAVIFA-EIPKTSTGKIQKFRLRE 541
Cdd:cd05937 411 EESSAVPTEFTKsllaSLARKNLPSYAVPLFLRLTeEVATTDNHKQQKGVLRD 463
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
20-534 |
5.87e-13 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 71.52 E-value: 5.87e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 20 TPLTY--LERAAKTYPDHIAIIH-----GRQR-ISYRDFWRRSLKVASALQKRGIGKGDTVTVMLsntPPMLEAHFgvpm 91
Cdd:PRK10524 52 TNLCHnaVDRHLAKRPEQLALIAvstetDEERtYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYM---PMIAEAAF---- 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 92 tkAVL------------------HSLNTRLDAAviafqldhaeTKVLIVDRE----------FSGVVRQALDLAKVTP-- 141
Cdd:PRK10524 125 --AMLacarigaihsvvfggfasHSLAARIDDA----------KPVLIVSADagsrggkvvpYKPLLDEAIALAQHKPrh 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 142 -LVIdyddpdyaadapypkgERigALDYEDFVAGGDEDFAwsmpddEWDAISLN-----------------YTSGTTGNP 203
Cdd:PRK10524 193 vLLV----------------DR--GLAPMARVAGRDVDYA------TLRAQHLGarvpvewlesnepsyilYTSGTTGKP 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 204 KGVVYHHRG--AALMAYTNTIHAGMAKHAvylwtlpMFHCN--GWCFPWTLAVQA------------GTHVclrwvRPKP 267
Cdd:PRK10524 249 KGVQRDTGGyaVALATSMDTIFGGKAGET-------FFCASdiGWVVGHSYIVYApllagmatimyeGLPT-----RPDA 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 268 -IYDAIAD-HGVTHLCGAPVVMSVLiNARDED---KRTFAQTVTFNTAAAPPPEAVLSGMADA-GFAVTHLYGLTETYGP 341
Cdd:PRK10524 317 gIWWRIVEkYKVNRMFSAPTAIRVL-KKQDPAllrKHDLSSLRALFLAGEPLDEPTASWISEAlGVPVIDNYWQTETGWP 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 342 AVVnewhgewddLAKGeRSAKKARQGVRYAALEG--LTVMDPETMQATPADgeTIGEVMFRGNIVmKGYLKNRKASDEAF 419
Cdd:PRK10524 396 ILA---------IARG-VEDRPTRLGSPGVPMYGynVKLLNEVTGEPCGPN--EKGVLVIEGPLP-PGCMQTVWGDDDRF 462
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 420 AGGWFHS--------GDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYV 491
Cdd:PRK10524 463 VKTYWSLfgrqvystFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFV 542
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|..
gi 499217198 492 ELKPGKT--------ASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKI 534
Cdd:PRK10524 543 VPKDSDSladrearlALEKEIMALVDSQLGAVARPARVWFVSaLPKTRSGKL 594
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
33-539 |
6.20e-13 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 70.97 E-value: 6.20e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 33 PDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQL 112
Cdd:cd17656 2 PDAVAVVFENQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIM 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 113 DHAETKVLIVDREFSgvvrQALDLAKVTPLvidyddpdyaadapypkgerigaLDYEDFVAGGDEDFAWSMPDDewDAIS 192
Cdd:cd17656 82 LDSGVRVVLTQRHLK----SKLSFNKSTIL-----------------------LEDPSISQEDTSNIDYINNSD--DLLY 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 193 LNYTSGTTGNPKGVVYHHRGAA-LMAYTNTIHAGMAKHAVYLWTLPMFHC------NGWCFPWTL-AVQAGTHVCLRwvr 264
Cdd:cd17656 133 IIYTSGTTGKPKGVQLEHKNMVnLLHFEREKTNINFSDKVLQFATCSFDVcyqeifSTLLSGGTLyIIREETKRDVE--- 209
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 265 pkPIYDAIADHGVTHLCgAPVVMSVLINARDEDKRTFAQTVTFNTAAAPppEAVLSG-----MADAGFAVTHLYGLTETY 339
Cdd:cd17656 210 --QLFDLVKRHNIEVVF-LPVAFLKFIFSEREFINRFPTCVKHIITAGE--QLVITNefkemLHEHNVHLHNHYGPSETH 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 340 gpaVVNEW---HG-EWDDLAKGERSAKKARqgvryaalegLTVMDpETMQATPADgeTIGEVMFRGNIVMKGYLKNRKAS 415
Cdd:cd17656 285 ---VVTTYtinPEaEIPELPPIGKPISNTW----------IYILD-QEQQLQPQG--IVGELYISGASVARGYLNRQELT 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 416 DEAFAGGWFHS-------GDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPV 488
Cdd:cd17656 349 AEKFFPDPFDPnermyrtGDLARYLPDGNIEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKGEKYLC 428
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 499217198 489 AYVelKPGKTASEAEIIEHCRALLARFKVPKAVIFAE-IPKTSTGKIQKFRL 539
Cdd:cd17656 429 AYF--VMEQELNISQLREYLAKQLPEYMIPSFFVPLDqLPLTPNGKVDRKAL 478
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
195-470 |
6.46e-13 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 71.30 E-value: 6.46e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 195 YTSGTTGNPKGVVYHHRG-AALMAYTNTIHAGMAKHAVYLWTLPMFH---------------CNGWCFPWTLA-----VQ 253
Cdd:PLN02387 257 YTSGSTGLPKGVMMTHGNiVATVAGVMTVVPKLGKNDVYLAYLPLAHilelaaesvmaavgaAIGYGSPLTLTdtsnkIK 336
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 254 AGTHvclrwvrpkpiydaiADhgVTHLcgAPVVMS----VLINARD------EDKRTFAQTVtFNTA------------- 310
Cdd:PLN02387 337 KGTK---------------GD--ASAL--KPTLMTavpaILDRVRDgvrkkvDAKGGLAKKL-FDIAykrrlaaiegswf 396
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 311 AAPPPEAV---------------------LSGMA----DA--------GFAVTHLYGLTETYGPAVVNEWhgewDDLAKG 357
Cdd:PLN02387 397 GAWGLEKLlwdalvfkkiravlggrirfmLSGGAplsgDTqrfiniclGAPIGQGYGLTETCAGATFSEW----DDTSVG 472
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 358 ersakkaRQG----VRYAAL----EG--LTVMDPetmqaTPAdgetiGEVMFRGNIVMKGYLKNRKASDEAF-----AGG 422
Cdd:PLN02387 473 -------RVGpplpCCYVKLvsweEGgyLISDKP-----MPR-----GEIVIGGPSVTLGYFKNQEKTDEVYkvderGMR 535
|
330 340 350 360
....*....|....*....|....*....|....*....|....*....
gi 499217198 423 WFHSGDLGVMHPDGYIQLKDRSKDII-ISGGENISSIEVEDALYKHPSV 470
Cdd:PLN02387 536 WFYTGDIGQFHPDGCLEIIDRKKDIVkLQHGEYVSLGKVEAALSVSPYV 584
|
|
| PRK07769 |
PRK07769 |
long-chain-fatty-acid--CoA ligase; Validated |
1-534 |
6.93e-13 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 181109 [Multi-domain] Cd Length: 631 Bit Score: 71.30 E-value: 6.93e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 1 MANSYEQDLDRNAANHQP--LTPLTYLERAAKTYPDHIA---IIHGRQR------ISYRDFWRRSLKVASALQKrgIGK- 68
Cdd:PRK07769 1 MNMAFHNPFDVNGKIRFPpnTNLVRHVERWAKVRGDKLAyrfLDFSTERdgvardLTWSQFGARNRAVGARLQQ--VTKp 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 69 GDTVTVMLSNTPPMLEAHFG--------VPMTKAVLHSLNTRLDAAviafqLDHAETKVLIVDREFSGVVRQALDL--AK 138
Cdd:PRK07769 79 GDRVAILAPQNLDYLIAFFGalyagriaVPLFDPAEPGHVGRLHAV-----LDDCTPSAILTTTDSAEGVRKFFRArpAK 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 139 VTPLVIdyddpdyaadapypkgerigALD-YEDFVAGgdedfAWSMPDDEWDAIS-LNYTSGTTGNPKGVVYHHRGAA-- 214
Cdd:PRK07769 154 ERPRVI--------------------AVDaVPDEVGA-----TWVPPEANEDTIAyLQYTSGSTRIPAGVQITHLNLPtn 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 215 LMAYTNTIHAGMAKHAVYlWtLPMFHCNGwCFPWTLAVQAGTHVCL-----------RWVR----------------PKP 267
Cdd:PRK07769 209 VLQVIDALEGQEGDRGVS-W-LPFFHDMG-LITVLLPALLGHYITFmspaafvrrpgRWIRelarkpggtggtfsaaPNF 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 268 IYDAIADHGVTHLCGAPVVMS---VLINArdEDKRTFAQTVTFNTAAAP---PPEAVLS--GMADAGFAVTHLYGLTEty 339
Cdd:PRK07769 286 AFEHAAARGLPKDGEPPLDLSnvkGLLNG--SEPVSPASMRKFNEAFAPyglPPTAIKPsyGMAEATLFVSTTPMDEE-- 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 340 gPAVVnewHGEWDDLAKG-----ERSAKKARQGV---RYAALEGLTVMDPETMQATPaDGEtIGEVMFRGNIVMKGYLKN 411
Cdd:PRK07769 362 -PTVI---YVDRDELNAGrfvevPADAPNAVAQVsagKVGVSEWAVIVDPETASELP-DGQ-IGEIWLHGNNIGTGYWGK 435
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 412 RKASDEAF------------AGG------WFHSGDLGVMHpDGYIQLKDRSKDIIISGGEN-------ISSIEVEDALyK 466
Cdd:PRK07769 436 PEETAATFqnilksrlseshAEGapddalWVRTGDYGVYF-DGELYITGRVKDLVIIDGRNhypqdleYTAQEATKAL-R 513
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 467 HPSVASCGVVA----------------RHDDKWGEVPVAYVELKPGKTASE-AEIIEHCRALLA-RFKVPKAVIF----A 524
Cdd:PRK07769 514 TGYVAAFSVPAnqlpqvvfddshaglkFDPEDTSEQLVIVAERAPGAHKLDpQPIADDIRAAIAvRHGVTVRDVLlvpaG 593
|
650
....*....|
gi 499217198 525 EIPKTSTGKI 534
Cdd:PRK07769 594 SIPRTSSGKI 603
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
22-535 |
3.69e-12 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 68.84 E-value: 3.69e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 22 LTYLERA--AKTYPDHIAIIHG----RQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEA---------- 85
Cdd:cd05943 70 LNYAENLlrHADADDPAAIYAAedgeRTEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAmlatasigai 149
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 86 ------HFGVPmtkAVLHSLnTRLDAAVIaFQLDHAETKVLIVDR--EFSGVVRQALDLAKVtpLVIDYDDPDYAADApy 157
Cdd:cd05943 150 wsscspDFGVP---GVLDRF-GQIEPKVL-FAVDAYTYNGKRHDVreKVAELVKGLPSLLAV--VVVPYTVAAGQPDL-- 220
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 158 pkGERIGALDYEDFVAGGDE---DFAWsMPDDewDAISLNYTSGTTGNPKGVVYHHRGAALMAYTNTI-HAGMAKHAVYL 233
Cdd:cd05943 221 --SKIAKALTLEDFLATGAAgelEFEP-LPFD--HPLYILYSSGTTGLPKCIVHGAGGTLLQHLKEHIlHCDLRPGDRLF 295
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 234 WtlpmFHCNGWCFpWT-----LAVqaGTHVCL---RWVRPKP--IYDAIADHGVTHLCGAPVVMSVLINARDEDKRTF-- 301
Cdd:cd05943 296 Y----YTTCGWMM-WNwlvsgLAV--GATIVLydgSPFYPDTnaLWDLADEEGITVFGTSAKYLDALEKAGLKPAETHdl 368
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 302 -AQTVTFNTAAAPPPE---------------AVLSGMAD--AGFAVThlygltetyGPAVvnewhgewdDLAKGERSAKK 363
Cdd:cd05943 369 sSLRTILSTGSPLKPEsfdyvydhikpdvllASISGGTDiiSCFVGG---------NPLL---------PVYRGEIQCRG 430
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 364 ARQGVRyaalegltVMDPEtmqATPADGEtIGE-----------VMFRGNivmKGYLKNRKASDEAFAGGWFHsGDLGVM 432
Cdd:cd05943 431 LGMAVE--------AFDEE---GKPVWGE-KGElvctkpfpsmpVGFWND---PDGSRYRAAYFAKYPGVWAH-GDWIEI 494
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 433 HPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASEaEIIEHCR--- 509
Cdd:cd05943 495 TPRGGVVILGRSDGTLNPGGVRIGTAEIYRVVEKIPEVEDSLVVGQEWKDGDERVILFVKLREGVELDD-ELRKRIRsti 573
|
570 580
....*....|....*....|....*....
gi 499217198 510 --ALLARFkVPKAVI-FAEIPKTSTGKIQ 535
Cdd:cd05943 574 rsALSPRH-VPAKIIaVPDIPRTLSGKKV 601
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
393-541 |
4.03e-12 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 68.67 E-value: 4.03e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 393 TIGEVMFRGNIVMKGYLKNRKASDEAF-AGGWFHSGDLGVMHpDGYIQLKDRSKDIIISGGENI-------SSIEVEDAL 464
Cdd:cd05908 339 YIGHIQIRGKNVTPGYYNNPEATAKVFtDDGWLKTGDLGFIR-NGRLVITGREKDIIFVNGQNVyphdierIAEELEGVE 417
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 465 YKHpsVASCGVvarHDDKWGEVPVAYVELKPGKTASEAEIIEHCRALLARFK---VPKAVIFAEIPKTSTGKIQKFRLRE 541
Cdd:cd05908 418 LGR--VVACGV---NNSNTRNEEIFCFIEHRKSEDDFYPLGKKIKKHLNKRGgwqINEVLPIRRIPKTTSGKVKRYELAQ 492
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
26-536 |
7.97e-12 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 67.19 E-value: 7.97e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 26 ERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDA 105
Cdd:cd17645 5 EEQVERTPDHVAVVDRGQSLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDYPG 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 106 AVIAFQLDHAETKVLIVDREfsgvvrqalDLAKVTplvidyddpdyaadapypkgerigaldyedfvaggdedfawsmpd 185
Cdd:cd17645 85 ERIAYMLADSSAKILLTNPD---------DLAYVI--------------------------------------------- 110
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 186 dewdaislnYTSGTTGNPKGV-VYHHRGAALMAYTNTIHAGMAKHAVYLWTLPMFHCNGW-CFPwTLAVQAGTHVCLRWV 263
Cdd:cd17645 111 ---------YTSGSTGLPKGVmIEHHNLVNLCEWHRPYFGVTPADKSLVYASFSFDASAWeIFP-HLTAGAALHVVPSER 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 264 RpkpiYDAIA------DHGVThlcgapvvMSVLINARDE-----DKRTFAQTVTFNTaaapppeaVLSGMADAGFAVTHL 332
Cdd:cd17645 181 R----LDLDAlndyfnQEGIT--------ISFLPTGAAEqfmqlDNQSLRVLLTGGD--------KLKKIERKGYKLVNN 240
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 333 YGLTETYGPAVVNEWHGEWDDLAKGersakKARQGVRYAALEGLTVMDPETMQatpadgetiGEVMFRGNIVMKGYLKNR 412
Cdd:cd17645 241 YGPTENTVVATSFEIDKPYANIPIG-----KPIDNTRVYILDEALQLQPIGVA---------GELCIAGEGLARGYLNRP 306
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 413 KASDEAFAGGWF-------HSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGE 485
Cdd:cd17645 307 ELTAEKFIVHPFvpgermyRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDADGRK 386
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 499217198 486 VPVAYVelKPGKTASEAEIIEHCRALLARFKVPKA-VIFAEIPKTSTGKIQK 536
Cdd:cd17645 387 YLVAYV--TAPEEIPHEELREWLKNDLPDYMIPTYfVHLKALPLTANGKVDR 436
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
18-536 |
9.17e-12 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 68.27 E-value: 9.17e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 18 PLTPLTYLERAAKTYPDHIAI-----IHGRQRI-SYRDFWRRSLKVASALQKRGiGKGDTVTVMLSNTPPMLEAHFGVpm 91
Cdd:PRK05691 8 PLTLVQALQRRAAQTPDRLALrfladDPGEGVVlSYRDLDLRARTIAAALQARA-SFGDRAVLLFPSGPDYVAAFFGC-- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 92 tkavlhslntrLDAAVIAFQ------------------LDHAETKVLIVDREFSGVVRQ--ALDLAKVTPLVidyddpdy 151
Cdd:PRK05691 85 -----------LYAGVIAVPayppesarrhhqerllsiIADAEPRLLLTVADLRDSLLQmeELAAANAPELL-------- 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 152 aadapypkgerigALDYEDFVAGGDedfaWSMPDDEWDAIS-LNYTSGTTGNPKGV-VYHHRgaaLMAYTNTIHAGMAKH 229
Cdd:PRK05691 146 -------------CVDTLDPALAEA----WQEPALQPDDIAfLQYTSGSTALPKGVqVSHGN---LVANEQLIRHGFGID 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 230 A----VYLWTLPMFHCNGWCFPWTLAVQAGTHVCLR-----WVRPKPIYDAIADHGVT---------HLCGAPVVMSVLI 291
Cdd:PRK05691 206 LnpddVIVSWLPLYHDMGLIGGLLQPIFSGVPCVLMspayfLERPLRWLEAISEYGGTisggpdfayRLCSERVSESALE 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 292 N---------------ARDEDKRTFAQTVTfntAAAPPPEAVLS--GMADAGFAVThlyGLTETYGPAVVnewhgEWDDL 354
Cdd:PRK05691 286 RldlsrwrvaysgsepIRQDSLERFAEKFA---ACGFDPDSFFAsyGLAEATLFVS---GGRRGQGIPAL-----ELDAE 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 355 AKGERSAKKARQGV-----RYAALEGLTVMDPETMQATPADgeTIGEVMFRGNIVMKGYLKNRKASDEAF---AG-GWFH 425
Cdd:PRK05691 355 ALARNRAEPGTGSVlmscgRSQPGHAVLIVDPQSLEVLGDN--RVGEIWASGPSIAHGYWRNPEASAKTFvehDGrTWLR 432
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 426 SGDLGVMHpDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVA----RHDDKWGEVPVAYVELKPGKTASE 501
Cdd:PRK05691 433 TGDLGFLR-DGELFVTGRLKDMLIVRGHNLYPQDIEKTVEREVEVVRKGRVAafavNHQGEEGIGIAAEISRSVQKILPP 511
|
570 580 590 600
....*....|....*....|....*....|....*....|
gi 499217198 502 AEIIEHCRALLARF--KVPKAVIFAE---IPKTSTGKIQK 536
Cdd:PRK05691 512 QALIKSIRQAVAEAcqEAPSVVLLLNpgaLPKTSSGKLQR 551
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
395-544 |
1.86e-11 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 66.66 E-value: 1.86e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 395 GEVMFRGNIVMKGYLKNRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDRSKDII-ISGGENISSIEVEDALYKHPSVAS 472
Cdd:PLN02736 459 GEICVRGPIIFKGYYKDEVQTREVIdEDGWLHTGDIGLWLPGGRLKIIDRKKNIFkLAQGEYIAPEKIENVYAKCKFVAQ 538
|
90 100 110 120 130 140 150
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 499217198 473 CGVvarHDDKWGEVPVAYV----ELKPGKTASEAEIIEHCRALLARFKVPKAViFAEIpkTSTGKIQKFRLREMAK 544
Cdd:PLN02736 539 CFV---YGDSLNSSLVAVVvvdpEVLKAWAASEGIKYEDLKQLCNDPRVRAAV-LADM--DAVGREAQLRGFEFAK 608
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
54-540 |
3.40e-11 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 65.82 E-value: 3.40e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 54 SLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVpMTKAVLHSL-NTRLDAAVIAFQLDHAETKVLIV-----DREFS 127
Cdd:PRK06060 40 AARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLAC-LARGVMAFLaNPELHRDDHALAARNTEPALVVTsdalrDRFQP 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 128 GVVRQALDLakvtplvidyddpdyaadapYPKGERIGALDYEdfVAGGDEdFAWSmpddewdaislNYTSGTTGNPKGVV 207
Cdd:PRK06060 119 SRVAEAAEL--------------------MSEAARVAPGGYE--PMGGDA-LAYA-----------TYTSGTTGPPKAAI 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 208 yhHRGAALMAYTNtihaGMAKHAVYLWTLPMFHCNGWCF--------PWTLAVQAGTHVclrwVRPKPIYDAIADHGVTH 279
Cdd:PRK06060 165 --HRHADPLTFVD----AMCRKALRLTPEDTGLCSARMYfayglgnsVWFPLATGGSAV----INSAPVTPEAAAILSAR 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 280 -----LCGAPVVMSVLINARDEDkrTFAQTVTFNTAAapppEAVLSGMAD------AGFAVTHLYGLTE---TYGPAVVN 345
Cdd:PRK06060 235 fgpsvLYGVPNFFARVIDSCSPD--SFRSLRCVVSAG----EALELGLAErlmeffGGIPILDGIGSTEvgqTFVSNRVD 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 346 EWhgewddlakgersakkarqgvRYAALEglTVMDPETMQATPADGETIG-----EVMFRGNIVMKGYLkNRKASDEAfA 420
Cdd:PRK06060 309 EW---------------------RLGTLG--RVLPPYEIRVVAPDGTTAGpgvegDLWVRGPAIAKGYW-NRPDSPVA-N 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 421 GGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTAS 500
Cdd:PRK06060 364 EGWLDTRDRVCIDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVAVRESTGASTLQAFLVATSGATID 443
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 499217198 501 EAEIIEHCRALLAR---FKVP-KAVIFAEIPKTSTGKIQKFRLR 540
Cdd:PRK06060 444 GSVMRDLHRGLLNRlsaFKVPhRFAVVDRLPRTPNGKLVRGALR 487
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
42-546 |
5.90e-11 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 65.37 E-value: 5.90e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 42 RQRISYRDFWRRSLkVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLI 121
Cdd:PRK06814 656 NGPLTYRKLLTGAF-VLGRKLKKNTPPGENVGVMLPNANGAAVTFFALQSAGRVPAMINFSAGIANILSACKAAQVKTVL 734
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 122 VDREFsgvvrqaLDLAKVTPLVidyddpdyaadAPYPKGERIGALdyEDF-------------VAGGDEDFAWSMPDDEW 188
Cdd:PRK06814 735 TSRAF-------IEKARLGPLI-----------EALEFGIRIIYL--EDVraqigladkikglLAGRFPLVYFCNRDPDD 794
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 189 DAISLnYTSGTTGNPKGVVYHHRgaALMAYTNTIHAGMAKHA--VYLWTLPMFHCNGWCFPWTLAVQAGTHVCL------ 260
Cdd:PRK06814 795 PAVIL-FTSGSEGTPKGVVLSHR--NLLANRAQVAARIDFSPedKVFNALPVFHSFGLTGGLVLPLLSGVKVFLypsplh 871
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 261 -RWVrPKPIYDAiadhgvthlcGAPVVMSvlinardedkrtfaqTVTFNTAAAPPPEA--------VLSG---------- 321
Cdd:PRK06814 872 yRII-PELIYDT----------NATILFG---------------TDTFLNGYARYAHPydfrslryVFAGaekvkeetrq 925
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 322 --MADAGFAVTHLYGLTETyGPAV-VNEwhgewdDLAKGERSAKKARQGVRYAaLEgltvmdpetmqatPADG-ETIGEV 397
Cdd:PRK06814 926 twMEKFGIRILEGYGVTET-APVIaLNT------PMHNKAGTVGRLLPGIEYR-LE-------------PVPGiDEGGRL 984
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 398 MFRGNIVMKGYLK-NRKASDEAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVED---ALYkhPSVASc 473
Cdd:PRK06814 985 FVRGPNVMLGYLRaENPGVLEPPADGWYDTGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEElaaELW--PDALH- 1061
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 499217198 474 GVVARHDDKWGEvpvAYVELKPGKTASEAEIIEHCRAL-LARFKVPKAVI-FAEIPKTSTGKIQKFRLREMAKLA 546
Cdd:PRK06814 1062 AAVSIPDARKGE---RIILLTTASDATRAAFLAHAKAAgASELMVPAEIItIDEIPLLGTGKIDYVAVTKLAEEA 1133
|
|
| PRK12476 |
PRK12476 |
putative fatty-acid--CoA ligase; Provisional |
9-534 |
1.19e-10 |
|
putative fatty-acid--CoA ligase; Provisional
Pssm-ID: 171527 [Multi-domain] Cd Length: 612 Bit Score: 63.99 E-value: 1.19e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 9 LDRNAANhqpltpltYLERAAKTYPDHIAIIHGRQ-RISYRDFWRRSLKVASALQkRGIGKGDTVTVMlsnTPPMLEAHF 87
Cdd:PRK12476 40 IERNIAN--------VGDTVAYRYLDHSHSAAGCAvELTWTQLGVRLRAVGARLQ-QVAGPGDRVAIL---APQGIDYVA 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 88 G-----------VPMTKAVLHSLNTRLDAAviafqLDHAETKVLIVDREFSGVVRQALDlakvtplvidyddpdyaaDAP 156
Cdd:PRK12476 108 GffaaikagtiaVPLFAPELPGHAERLDTA-----LRDAEPTVVLTTTAAAEAVEGFLR------------------NLP 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 157 YPKGERIGALDYEDFVAGgdEDFAWSMPDDewDAIS-LNYTSGTTGNPKGVVYHHRGAAlmayTNTIHA-------GMAK 228
Cdd:PRK12476 165 RLRRPRVIAIDAIPDSAG--ESFVPVELDT--DDVShLQYTSGSTRPPVGVEITHRAVG----TNLVQMilsidllDRNT 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 229 HAVYlWtLPMFHCNGWC---FPwtlAVqAGTHVCL-----------RWVR---------------PKPIYDAIADHGVTH 279
Cdd:PRK12476 237 HGVS-W-LPLYHDMGLSmigFP---AV-YGGHSTLmsptafvrrpqRWIKalsegsrtgrvvtaaPNFAYEWAAQRGLPA 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 280 lCGAPVVMS--VLINArdEDKRTFAQTVTFNTAAAP---PPEAVLS--GMADAGFAVTHLYGLTEtygPAVVnewHGEWD 352
Cdd:PRK12476 311 -EGDDIDLSnvVLIIG--SEPVSIDAVTTFNKAFAPyglPRTAFKPsyGIAEATLFVATIAPDAE---PSVV---YLDRE 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 353 DLAKGE--RSAKKARQGV------RYAALEGLTVMDPETMQATPaDGEtIGEVMFRGNIVMKGYLKNRKASDEAF----- 419
Cdd:PRK12476 382 QLGAGRavRVAADAPNAVahvscgQVARSQWAVIVDPDTGAELP-DGE-VGEIWLHGDNIGRGYWGRPEETERTFgaklq 459
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 420 --------AGG------WFHSGDLGVmHPDGYIQLKDRSKDIIISGGEN--ISSIE--VEDAlykHPSVASCGVVA---R 478
Cdd:PRK12476 460 srlaegshADGaaddgtWLRTGDLGV-YLDGELYITGRIADLIVIDGRNhyPQDIEatVAEA---SPMVRRGYVTAftvP 535
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 499217198 479 HDDKWGEVPVAyvELKPGKTASE-AEIIEHCRALLA-RFKVPKA----VIFAEIPKTSTGKI 534
Cdd:PRK12476 536 AEDNERLVIVA--ERAAGTSRADpAPAIDAIRAAVSrRHGLAVAdvrlVPAGAIPRTTSGKL 595
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
422-536 |
5.20e-10 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 62.07 E-value: 5.20e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 422 GWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDDKWGEVPVAYVELKPGKTASE 501
Cdd:PTZ00237 492 GYYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKHPLVLECCSIGIYDPDCYNVPIGLLVLKQDQSNQS 571
|
90 100 110 120
....*....|....*....|....*....|....*....|....*
gi 499217198 502 A----------EIIEHCRALLARFKvpKAVIFAEIPKTSTGKIQK 536
Cdd:PTZ00237 572 IdlnklkneinNIITQDIESLAVLR--KIIIVNQLPKTKTGKIPR 614
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
41-482 |
9.79e-10 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 61.15 E-value: 9.79e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 41 GRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVL 120
Cdd:PTZ00216 118 ETRYITYAELWERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSMVAATVYANLGEDALAYALRETECKAI 197
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 121 IVDREFSGVVRQALDLAKVTPLVIDYDDPDYAADAPypKGERIGAldYEDFVAGGDE---DFAWSMPDDEWDAISLNYTS 197
Cdd:PTZ00216 198 VCNGKNVPNLLRLMKSGGMPNTTIIYLDSLPASVDT--EGCRLVA--WTDVVAKGHSagsHHPLNIPENNDDLALIMYTS 273
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 198 GTTGNPKGVVYHHRgaALMAYTNTIH-------AGMAKHAVYLWTLPMFHC------N---------GWCFPWTLA-VQA 254
Cdd:PTZ00216 274 GTTGDPKGVMHTHG--SLTAGILALEdrlndliGPPEEDETYCSYLPLAHImefgvtNiflargaliGFGSPRTLTdTFA 351
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 255 GTHVCLRWVRPK-----P-IYDAIA------------------DHGVTHLCGApvvmsvLINARDedkrtfaqTVTFNTA 310
Cdd:PTZ00216 352 RPHGDLTEFRPVfligvPrIFDTIKkaveaklppvgslkrrvfDHAYQSRLRA------LKEGKD--------TPYWNEK 417
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 311 AAPPPEAVLSG----MADAG--------------FA-VTHLYGLTETygpaVVNewhgewddlakgersAKKARQGVRYA 371
Cdd:PTZ00216 418 VFSAPRAVLGGrvraMLSGGgplsaatqefvnvvFGmVIQGWGLTET----VCC---------------GGIQRTGDLEP 478
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 372 ALEGLTVMDPEtMQATPADG-------ETIGEVMFRGNIVMKGYLKNRKASDEAF-AGGWFHSGDLGVMHPDGYIQLKDR 443
Cdd:PTZ00216 479 NAVGQLLKGVE-MKLLDTEEykhtdtpEPRGEILLRGPFLFKGYYKQEELTREVLdEDGWFHTGDVGSIAANGTLRIIGR 557
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 499217198 444 SKDIII-SGGENIsSIEVEDALY-KHPSVASCGV-VARHDDK 482
Cdd:PTZ00216 558 VKALAKnCLGEYI-ALEALEALYgQNELVVPNGVcVLVHPAR 598
|
|
| PRK05850 |
PRK05850 |
acyl-CoA synthetase; Validated |
193-545 |
1.57e-08 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235624 [Multi-domain] Cd Length: 578 Bit Score: 57.26 E-value: 1.57e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 193 LNYTSGTTGNPKGVVYHHRG---------AALMAYTNTIHAgmAKHAVYLWtLPMFH----CNGWCFPwtlaVQAGTHVC 259
Cdd:PRK05850 165 LQYTSGSTRTPAGVMVSHRNvianfeqlmSDYFGDTGGVPP--PDTTVVSW-LPFYHdmglVLGVCAP----ILGGCPAV 237
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 260 L-----------RWVRpkpiydAIADHGVTHLCgAPVVMSVLINAR--DED----------------KRTFAQTVT-FNT 309
Cdd:PRK05850 238 LtspvaflqrpaRWMQ------LLASNPHAFSA-APNFAFELAVRKtsDDDmagldlggvlgiisgsERVHPATLKrFAD 310
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 310 AAAP---PPEAVLS--GMADAGfavthLYGLTETYG--PAVVnewHGEWDDLAKGERSAKKARQG---VRYAALEGLTV- 378
Cdd:PRK05850 311 RFAPfnlRETAIRPsyGLAEAT-----VYVATREPGqpPESV---RFDYEKLSAGHAKRCETGGGtplVSYGSPRSPTVr 382
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 379 -MDPETMQATPADgeTIGEVMFRGNIVMKGYLKNRKASDEAF------------AGGWFHSGDLGVMHpDGYIQLKDRSK 445
Cdd:PRK05850 383 iVDPDTCIECPAG--TVGEIWVHGDNVAAGYWQKPEETERTFgatlvdpspgtpEGPWLRTGDLGFIS-EGELFIVGRIK 459
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 446 DIIISGGENISSIEVEdALYKHPSVASCGVVARHDDKwGEVPVAYVELKPgKTASEAEIIEHCRAL----------LARF 515
Cdd:PRK05850 460 DLLIVDGRNHYPDDIE-ATIQEITGGRVAAISVPDDG-TEKLVAIIELKK-RGDSDEEAMDRLRTVkrevtsaiskSHGL 536
|
410 420 430
....*....|....*....|....*....|....*...
gi 499217198 516 KVPKAVIFA--EIPKTSTGKI------QKFRLREMAKL 545
Cdd:PRK05850 537 SVADLVLVApgSIPITTSGKIrraacvEQYRQDEFTRL 574
|
|
| PRK12582 |
PRK12582 |
acyl-CoA synthetase; Provisional |
25-451 |
2.05e-08 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237144 [Multi-domain] Cd Length: 624 Bit Score: 56.98 E-value: 2.05e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAII-----HGRQR-ISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTppmLEAHFgvpMTKAVLHS 98
Cdd:PRK12582 55 LAKWAAEAPDRPWLAqrepgHGQWRkVTYGEAKRAVDALAQALLDLGLDPGRPVMILSGNS---IEHAL---MTLAAMQA 128
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 99 lntRLDAAVI--AFQL---DHAETKVLiVDREFSGVV-----------RQALDLAKVTPLVIDYDdpdyaadapypkGER 162
Cdd:PRK12582 129 ---GVPAAPVspAYSLmshDHAKLKHL-FDLVKPRVVfaqsgapfaraLAALDLLDVTVVHVTGP------------GEG 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 163 IGALDYEDFVAGG-DEDFAWSMPDDEWDAIS-LNYTSGTTGNPKGVVYHHR---GAALMAYTNTIHAGMAKHAVYLWTLP 237
Cdd:PRK12582 193 IASIAFADLAATPpTAAVAAAIAAITPDTVAkYLFTSGSTGMPKAVINTQRmmcANIAMQEQLRPREPDPPPPVSLDWMP 272
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 238 MFHCNGWCFPWTLAVQAGTHVCLRWVRPKP--IYDAIAD-HGV--THLCGAPVVMSVLINARDED----KRTFAQTVTFN 308
Cdd:PRK12582 273 WNHTMGGNANFNGLLWGGGTLYIDDGKPLPgmFEETIRNlREIspTVYGNVPAGYAMLAEAMEKDdalrRSFFKNLRLMA 352
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 309 TAAAPPPEAVLSGMADAGFAVT-------HLYGLTETYGPAVVNEWHGEwddlakgersakkaRQGVRYAALEGLTV-MD 380
Cdd:PRK12582 353 YGGATLSDDLYERMQALAVRTTghripfyTGYGATETAPTTTGTHWDTE--------------RVGLIGLPLPGVELkLA 418
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 499217198 381 PetmqatpaDGETIgEVMFRGNIVMKGYLKNRKASDEAF-AGGWFHSGDLGVmhpdgYIQLKDRSKDIIISG 451
Cdd:PRK12582 419 P--------VGDKY-EVRVKGPNVTPGYHKDPELTAAAFdEEGFYRLGDAAR-----FVDPDDPEKGLIFDG 476
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
186-467 |
6.84e-08 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 55.21 E-value: 6.84e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 186 DEWDAISLNYTSGTTGNPKGVVYHHrgAALMAYTNTIHAGMA--KHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLRW- 262
Cdd:PRK06334 181 DPEDVAVILFTSGTEKLPKGVPLTH--ANLLANQRACLKFFSpkEDDVMMSFLPPFHAYGFNSCTLFPLLSGVPVVFAYn 258
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 263 -VRPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAqTVTFNTAAAPPPEAVLSGMADAGFAVTHL---YGLTET 338
Cdd:PRK06334 259 pLYPKKIVEMIDEAKVTFLGSTPVFFDYILKTAKKQESCLP-SLRFVVIGGDAFKDSLYQEALKTFPHIQLrqgYGTTEC 337
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 339 YGPAVVNEwhgewddlakgERSAKKAR-QGVryaALEGLTVMDPETMQATPADGETIGEVMFRGNIVMKGYLKNRKASDE 417
Cdd:PRK06334 338 SPVITINT-----------VNSPKHEScVGM---PIRGMDVLIVSEETKVPVSSGETGLVLTRGTSLFSGYLGEDFGQGF 403
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|..
gi 499217198 418 AFAGG--WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKH 467
Cdd:PRK06334 404 VELGGetWYVTGDLGYVDRHGELFLKGRLSRFVKIGAEMVSLEALESILMEG 455
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
43-540 |
9.20e-08 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 54.74 E-value: 9.20e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 43 QRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGvpMTK----AVLHSLNTRLDAAVIAFQLDHAetK 118
Cdd:cd05939 2 RHWTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLG--LAKigveTALINSNLRLESLLHCITVSKA--K 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 119 VLIVDrefsgvVRQALDLAKVTPLVidyddpdyaadapypkgerigaldyedfvaggdedfawSMPDDEWDAISLN-YTS 197
Cdd:cd05939 78 ALIFN------LLDPLLTQSSTEPP--------------------------------------SQDDVNFRDKLFYiYTS 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 198 GTTGNPKGVVYHH----RGAALMAYTntihAGMAKHAVYLWTLPMFHcngwcfpwtlavQAGTHVClrwvrpkpIYDAIA 273
Cdd:cd05939 114 GTTGLPKAAVIVHsryyRIAAGAYYA----FGMRPEDVVYDCLPLYH------------SAGGIMG--------VGQALL 169
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 274 dHGvthlcgapvvMSVLINARDEDKRTFAQTVTFNTA-------------AAPPPEAvlsgmaDAGFAVTHLYGltetyg 340
Cdd:cd05939 170 -HG----------STVVIRKKFSASNFWDDCVKYNCTivqyigeicryllAQPPSEE------EQKHNVRLAVG------ 226
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 341 pavvNEWHGE-WDDLAKGERSAKKarqGVRYAALEG----------------------------LTVMDPETMQA----- 386
Cdd:cd05939 227 ----NGLRPQiWEQFVRRFGIPQI---GEFYGATEGnsslvnidnhvgacgfnsrilpsvypirLIKVDEDTGELirdsd 299
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 387 ------TPAD-GETIGEVMfRGNIVMK--GYLK----NRKASDEAFAGG--WFHSGDLGVMHPDGYIQLKDRSKDIIISG 451
Cdd:cd05939 300 glcipcQPGEpGLLVGKII-QNDPLRRfdGYVNegatNKKIARDVFKKGdsAFLSGDVLVMDELGYLYFKDRTGDTFRWK 378
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 452 GENISSIEVEDALYKHPSVASCGVvarhddkWGeVPVAYVELKPGKTA----SEAEIIEHCRALLARFKVPKAV-----I 522
Cdd:cd05939 379 GENVSTTEVEGILSNVLGLEDVVV-------YG-VEVPGVEGRAGMAAivdpERKVDLDRFSAVLAKSLPPYARpqfirL 450
|
570
....*....|....*...
gi 499217198 523 FAEIPKTSTGKIQKFRLR 540
Cdd:cd05939 451 LPEVDKTGTFKLQKTDLQ 468
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
33-212 |
2.70e-07 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 53.26 E-value: 2.70e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 33 PDHIAIIH-----GRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPP----MLEA------------HFGVPm 91
Cdd:PRK03584 98 DDRPAIIFrgedgPRRELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPEtvvaMLATaslgaiwsscspDFGVQ- 176
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 92 tkavlhslntrldaAVIA-F-QLdhaETKVLI-VD------REF--SGVVRQALD-LAKVTPLVIDYDDPDYAADAPYPk 159
Cdd:PRK03584 177 --------------GVLDrFgQI---EPKVLIaVDgyryggKAFdrRAKVAELRAaLPSLEHVVVVPYLGPAAAAAALP- 238
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....*...
gi 499217198 160 geriGALDYEDFVAGGDE---DFAWsMPDDE--WdaisLNYTSGTTGNPKGVVYHHRG 212
Cdd:PRK03584 239 ----GALLWEDFLAPAEAaelEFEP-VPFDHplW----ILYSSGTTGLPKCIVHGHGG 287
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
25-541 |
3.43e-07 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 53.63 E-value: 3.43e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 25 LERAAKTYPDHIAIIHGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLD 104
Cdd:PRK05691 1137 LNEQARQTPERIALVWDGGSLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLDPDYP 1216
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 105 AAVIAFQLDHAETKVLIVDREFSGVVRQALDLAKVtplvidyddpdyaadapypkgerigALDYEDFvaggdEDFAWSMP 184
Cdd:PRK05691 1217 AERLAYMLADSGVELLLTQSHLLERLPQAEGVSAI-------------------------ALDSLHL-----DSWPSQAP 1266
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 185 DDEWDAISLNY---TSGTTGNPKGVVYHHrgAALMAYTNTIHAGMAKHA--VYLWTLPM-FHCNGW-CFpWTLAvqAGTH 257
Cdd:PRK05691 1267 GLHLHGDNLAYviyTSGSTGQPKGVGNTH--AALAERLQWMQATYALDDsdVLMQKAPIsFDVSVWeCF-WPLI--TGCR 1341
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 258 VCLRWV----RPKPIYDAIADHGVTHLCGAPVVMSVLINARDEDKRTFAQTVtFNTAAAPPPE---AVLSGMadAGFAVT 330
Cdd:PRK05691 1342 LVLAGPgehrDPQRIAELVQQYGVTTLHFVPPLLQLFIDEPLAAACTSLRRL-FSGGEALPAElrnRVLQRL--PQVQLH 1418
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 331 HLYGLTETygpAV-VNEWHGEWDDlakGERSAKKarqgvRYAALEGLTVMDPETMQATPAdgeTIGEVMFRGNIVMKGYL 409
Cdd:PRK05691 1419 NRYGPTET---AInVTHWQCQAED---GERSPIG-----RPLGNVLCRVLDAELNLLPPG---VAGELCIGGAGLARGYL 1484
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 410 KNRKASDEAF-------AGG-WFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVAScGVVARHDD 481
Cdd:PRK05691 1485 GRPALTAERFvpdplgeDGArLYRTGDRARWNADGALEYLGRLDQQVKLRGFRVEPEEIQARLLAQPGVAQ-AAVLVREG 1563
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 499217198 482 KWGEVPVAYVELKPGKTASEAEIIEHCRALLARFKVPKAVI-FAEIPKTSTGKIQKFRLRE 541
Cdd:PRK05691 1564 AAGAQLVGYYTGEAGQEAEAERLKAALAAELPEYMVPAQLIrLDQMPLGPSGKLDRRALPE 1624
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
42-243 |
3.71e-06 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 49.76 E-value: 3.71e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 42 RQRISYRDFWRRSLKVASALQ-KRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVL 120
Cdd:cd17632 65 FETITYAELWERVGAVAAAHDpEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAPILAETEPRLL 144
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 121 IVDREFSGVVRQA-LDLAKVTPLVIDYDDPDYAADAPYPKGERIGALDY------EDFVAGGDEDF---AWSMPDDEWDA 190
Cdd:cd17632 145 AVSAEHLDLAVEAvLEGGTPPRLVVFDHRPEVDAHRAALESARERLAAVgipvttLTLIAVRGRDLppaPLFRPEPDDDP 224
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....*
gi 499217198 191 IS-LNYTSGTTGNPKGVVYHHRGAALMAYTNT-IHAGMAKHAVYLWTLPMFHCNG 243
Cdd:cd17632 225 LAlLIYTSGSTGTPKGAMYTERLVATFWLKVSsIQDIRPPASITLNFMPMSHIAG 279
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
46-543 |
5.35e-06 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 49.32 E-value: 5.35e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 46 SYRDFWRRSLKVASALQkRGIGKGDTVTVMLSNTPPMLEAHFGVPMTKAVLHSLNTRLDAAVIAFQLDHAETKVLIVDRE 125
Cdd:PRK08043 233 SYRKLLKKTLFVGRILE-KYSVEGERIGLMLPNATISAAVIFGASLRRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQ 311
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 126 FsgvvrqaLDLAKVTPLvidyddpdyaadAPYPKGERIGAL-DYEDFVAGGDEdfAW--------------SMPDDewDA 190
Cdd:PRK08043 312 F-------LDKGKLWHL------------PEQLTQVRWVYLeDLKDDVTTADK--LWifahllmprlaqvkQQPED--AA 368
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 191 ISLnYTSGTTGNPKGVVYHHRgaALMAYTNTIH--AGMAKHAVYLWTLPMFHCNGWCFPWTLAVQAGTHVCLrwvRPKPI 268
Cdd:PRK08043 369 LIL-FTSGSEGHPKGVVHSHK--SLLANVEQIKtiADFTPNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFL---YPSPL 442
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 269 Y-----DAIADHGVTHLCGAPvvmSVLIN-ARDEDKRTFAQTVTFNTAAAPPPEAVLSGMADA-GFAVTHLYGLTETyGP 341
Cdd:PRK08043 443 HyrivpELVYDRNCTVLFGTS---TFLGNyARFANPYDFARLRYVVAGAEKLQESTKQLWQDKfGLRILEGYGVTEC-AP 518
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 342 AV-VNewhgewddlakgersakkarqgVRYAALEG-----LTVMDPETMqatPADG-ETIGEVMFRGNIVMKGYLK---- 410
Cdd:PRK08043 519 VVsIN----------------------VPMAAKPGtvgriLPGMDARLL---SVPGiEQGGRLQLKGPNIMNGYLRvekp 573
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 411 ---------NRKASDEAfagGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPSVASCGVVARHDD 481
Cdd:PRK08043 574 gvlevptaeNARGEMER---GWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQLALGVSPDKQHATAIKSDA 650
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 499217198 482 KWGEVPVAY---VELKPGKTASEAEiiEHCRALLArfkVPKAVIF-AEIPKTSTGKIQKFRLREMA 543
Cdd:PRK08043 651 SKGEALVLFttdSELTREKLQQYAR--EHGVPELA---VPRDIRYlKQLPLLGSGKPDFVTLKSMV 711
|
|
| PTZ00342 |
PTZ00342 |
acyl-CoA synthetase; Provisional |
333-455 |
3.46e-05 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240370 [Multi-domain] Cd Length: 746 Bit Score: 46.64 E-value: 3.46e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 333 YGLTETYGPAVVNEWHGEWDDLAKGERSAKkarqgVRYaaleglTVMDPETMQATpaDGETIGEVMFRGNIVMKGYLKNR 412
Cdd:PTZ00342 493 YGLTETTGPIFVQHADDNNTESIGGPISPN-----TKY------KVRTWETYKAT--DTLPKGELLIKSDSIFSGYFLEK 559
|
90 100 110 120
....*....|....*....|....*....|....*....|....*
gi 499217198 413 KASDEAFA-GGWFHSGDLGVMHPDGYIQLKDRSKDII-ISGGENI 455
Cdd:PTZ00342 560 EQTKNAFTeDGYFKTGDIVQINKNGSLTFLDRSKGLVkLSQGEYI 604
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
17-211 |
7.98e-03 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 39.09 E-value: 7.98e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 17 QPLTPLT-----YLERAAKTYPDHIAII-----HGRQRISYRDFWRRSLKVASALQKRGIGKGDTVTVMLSNTPP----M 82
Cdd:PRK08180 32 EPLGDYPrrltdRLVHWAQEAPDRVFLAergadGGWRRLTYAEALERVRAIAQALLDRGLSAERPLMILSGNSIEhallA 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499217198 83 LEA-HFGVPMTK-AVLHSLNTRlDAAVIAFQLDHAETKVLIVDREfsGVVRQALDLAKV--TPLVIdyddpdyaadapyP 158
Cdd:PRK08180 112 LAAmYAGVPYAPvSPAYSLVSQ-DFGKLRHVLELLTPGLVFADDG--AAFARALAAVVPadVEVVA-------------V 175
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 499217198 159 KGERIG--ALDYEDFVA-----GGDEDFAWSMPDDewdaIS-LNYTSGTTGNPKGVVYHHR 211
Cdd:PRK08180 176 RGAVPGraATPFAALLAtpptaAVDAAHAAVGPDT----IAkFLFTSGSTGLPKAVINTHR 232
|
|
| |
