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Conserved domains on  [gi|499222169|ref|WP_010919709|]
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DsbA family oxidoreductase [Caulobacter vibrioides]

Protein Classification

DsbA family oxidoreductase( domain architecture ID 10122494)

DsbA family oxidoreductase belonging to the thioredoxin superfamily, similar to Deinococcus radiodurans FrnE, a cadmium-inducible disulfide isomerase chaperone that functions in oxidative stress tolerance

CATH:  3.40.30.10
EC:  1.8.-.-
Gene Ontology:  GO:0015036
SCOP:  3000031

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
DsbA_FrnE cd03024
DsbA family, FrnE subfamily; FrnE is a DsbA-like protein containing a CXXC motif. It is ...
9-209 7.00e-82

DsbA family, FrnE subfamily; FrnE is a DsbA-like protein containing a CXXC motif. It is presumed to be a thiol oxidoreductase involved in polyketide biosynthesis, specifically in the production of the aromatic antibiotics frenolicin and nanaomycins.


:

Pssm-ID: 239322 [Multi-domain]  Cd Length: 201  Bit Score: 242.10  E-value: 7.00e-82
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169   9 IDVVADVVCPWCYLGWRRLKSAIALRPD-LKAQLIWRPYQLDPTLPEDGVDRKAYMAQKFKDPEKLKAVHTALVEGGAEE 87
Cdd:cd03024    1 IDIWSDVVCPWCYIGKRRLEKALAELGDeVDVEIEWRPFELNPDMPPEGEDRREYLARKYGSTAEQAAAMRRVEAAAAAE 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169  88 GIVFNFEAIaLSPNTNAAHRLIRWALTAGVQDHVVEALFKAYFEQGLDIGDPIVLGDIAEAAGMERLVVLQLLSEGADKE 167
Cdd:cd03024   81 GLEFDFDRV-RPPNTFDAHRLIHLAKEQGKQDALVEALFRAYFTEGKDIGDRDVLVDLAEEAGLDAAEARAVLASDEYAD 159
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
gi 499222169 168 AVAREHAMAVQGGVTGVPFAIFAGKVAVVGAESPERIAQAID 209
Cdd:cd03024  160 EVRADEARARQLGISGVPFFVFNGKYAVSGAQPPEVFLQALR 201
 
Name Accession Description Interval E-value
DsbA_FrnE cd03024
DsbA family, FrnE subfamily; FrnE is a DsbA-like protein containing a CXXC motif. It is ...
9-209 7.00e-82

DsbA family, FrnE subfamily; FrnE is a DsbA-like protein containing a CXXC motif. It is presumed to be a thiol oxidoreductase involved in polyketide biosynthesis, specifically in the production of the aromatic antibiotics frenolicin and nanaomycins.


Pssm-ID: 239322 [Multi-domain]  Cd Length: 201  Bit Score: 242.10  E-value: 7.00e-82
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169   9 IDVVADVVCPWCYLGWRRLKSAIALRPD-LKAQLIWRPYQLDPTLPEDGVDRKAYMAQKFKDPEKLKAVHTALVEGGAEE 87
Cdd:cd03024    1 IDIWSDVVCPWCYIGKRRLEKALAELGDeVDVEIEWRPFELNPDMPPEGEDRREYLARKYGSTAEQAAAMRRVEAAAAAE 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169  88 GIVFNFEAIaLSPNTNAAHRLIRWALTAGVQDHVVEALFKAYFEQGLDIGDPIVLGDIAEAAGMERLVVLQLLSEGADKE 167
Cdd:cd03024   81 GLEFDFDRV-RPPNTFDAHRLIHLAKEQGKQDALVEALFRAYFTEGKDIGDRDVLVDLAEEAGLDAAEARAVLASDEYAD 159
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
gi 499222169 168 AVAREHAMAVQGGVTGVPFAIFAGKVAVVGAESPERIAQAID 209
Cdd:cd03024  160 EVRADEARARQLGISGVPFFVFNGKYAVSGAQPPEVFLQALR 201
FrnE COG2761
Predicted dithiol-disulfide isomerase, DsbA/YjbH family (virulence, stress resistance) ...
7-213 3.95e-74

Predicted dithiol-disulfide isomerase, DsbA/YjbH family (virulence, stress resistance) [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 442047 [Multi-domain]  Cd Length: 205  Bit Score: 222.84  E-value: 3.95e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169   7 MIIDVVADVVCPWCYLGWRRLKSAIALRPDlKAQLIWRPYQLDPTLPEDGVDRKAYMAQKfKDPEKLKAVHTALVEGGAE 86
Cdd:COG2761    2 LKIDIFSDVVCPWCYIGKRRLEKALAEFGD-DVEIRWRPFELNPDMPPEGEDRREYLLAK-GSPEQAEQMRAHVEEAAAE 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169  87 EGIVFNFEAIAlSPNTNAAHRLIRWALTAGVQDHVVEALFKAYFEQGLDIGDPIVLGDIAEAAGMERLVVLQLLSEGADK 166
Cdd:COG2761   80 EGLPFDFDRIK-PPNTFDAHRLLKAAELQGKQDALLEALFEAYFTEGRDIGDREVLLDLAAEVGLDAEEFRADLESDEAA 158
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*..
gi 499222169 167 EAVAREHAMAVQGGVTGVPFAIFAGKVAVVGAESPERIAQAIDQALA 213
Cdd:COG2761  159 AAVRADEAEARELGVTGVPTFVFDGKYAVSGAQPYEVFEQALRQALA 205
DSBA pfam01323
DSBA-like thioredoxin domain; This family contains a diverse set of proteins with a ...
8-209 1.56e-54

DSBA-like thioredoxin domain; This family contains a diverse set of proteins with a thioredoxin-like structure pfam00085. This family also includes 2-hydroxychromene-2-carboxylate (HCCA) isomerase enzymes catalyze one step in prokaryotic polyaromatic hydrocarbon (PAH) catabolic pathways. This family also contains members with functions other than HCCA isomerization, such as Kappa family GSTs, whose similarity to HCCA isomerases was not previously recognized. The sequence Swiss:O07298 has been annotated as a dioxygenase but is almost certainly an HCCA isomerase enzyme. Similarly, the sequence Swiss:Q9ZI67 has been annotated as a dehydrogenase, but is most probably also an HCCA isomerase enzyme. In addition, the Rhizobium leguminosarum Swiss:Q52782 protein has been annotated as a putative glycerol-3-phosphate transfer protein, but is also most likely to be an HCCA isomerase enzyme.


Pssm-ID: 426200 [Multi-domain]  Cd Length: 191  Bit Score: 172.61  E-value: 1.56e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169    8 IIDVVADVVCPWCYLGWRRLKSAIALRPDLKaqLIWRPYQLDPTLPEDGVDRKAymaqkfkDPEKLKAVHTALVEGGAEE 87
Cdd:pfam01323   1 TVDEFFDFLCPFCYLAKERLEKLAARYGDVK--VVYRPFPLAGAKKIGNVGPSN-------LPVKLKYMMADLERWAALY 71
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169   88 GIVFNFEAIALSpNTNAAHRLIRWALTAGVQDHVVEALFKAYFEQGLDIGDPIVLGDIAEAAGMERLVVLQLLSEGADKE 167
Cdd:pfam01323  72 GIPLRFPANFLG-NSTRANRLALAAGAEGLAEKVVRELFNALWGEGAAITDDSVLREVAEKAGLDAEEFDEFLDSPAVKE 150
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 499222169  168 AVAREHAMAVQGGVTGVPFAIFAGKvAVVGAESPERIAQAID 209
Cdd:pfam01323 151 AVRENTAAAISLGVFGVPTFVVGGK-MVFGADRLDTLADALA 191
 
Name Accession Description Interval E-value
DsbA_FrnE cd03024
DsbA family, FrnE subfamily; FrnE is a DsbA-like protein containing a CXXC motif. It is ...
9-209 7.00e-82

DsbA family, FrnE subfamily; FrnE is a DsbA-like protein containing a CXXC motif. It is presumed to be a thiol oxidoreductase involved in polyketide biosynthesis, specifically in the production of the aromatic antibiotics frenolicin and nanaomycins.


Pssm-ID: 239322 [Multi-domain]  Cd Length: 201  Bit Score: 242.10  E-value: 7.00e-82
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169   9 IDVVADVVCPWCYLGWRRLKSAIALRPD-LKAQLIWRPYQLDPTLPEDGVDRKAYMAQKFKDPEKLKAVHTALVEGGAEE 87
Cdd:cd03024    1 IDIWSDVVCPWCYIGKRRLEKALAELGDeVDVEIEWRPFELNPDMPPEGEDRREYLARKYGSTAEQAAAMRRVEAAAAAE 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169  88 GIVFNFEAIaLSPNTNAAHRLIRWALTAGVQDHVVEALFKAYFEQGLDIGDPIVLGDIAEAAGMERLVVLQLLSEGADKE 167
Cdd:cd03024   81 GLEFDFDRV-RPPNTFDAHRLIHLAKEQGKQDALVEALFRAYFTEGKDIGDRDVLVDLAEEAGLDAAEARAVLASDEYAD 159
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
gi 499222169 168 AVAREHAMAVQGGVTGVPFAIFAGKVAVVGAESPERIAQAID 209
Cdd:cd03024  160 EVRADEARARQLGISGVPFFVFNGKYAVSGAQPPEVFLQALR 201
FrnE COG2761
Predicted dithiol-disulfide isomerase, DsbA/YjbH family (virulence, stress resistance) ...
7-213 3.95e-74

Predicted dithiol-disulfide isomerase, DsbA/YjbH family (virulence, stress resistance) [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 442047 [Multi-domain]  Cd Length: 205  Bit Score: 222.84  E-value: 3.95e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169   7 MIIDVVADVVCPWCYLGWRRLKSAIALRPDlKAQLIWRPYQLDPTLPEDGVDRKAYMAQKfKDPEKLKAVHTALVEGGAE 86
Cdd:COG2761    2 LKIDIFSDVVCPWCYIGKRRLEKALAEFGD-DVEIRWRPFELNPDMPPEGEDRREYLLAK-GSPEQAEQMRAHVEEAAAE 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169  87 EGIVFNFEAIAlSPNTNAAHRLIRWALTAGVQDHVVEALFKAYFEQGLDIGDPIVLGDIAEAAGMERLVVLQLLSEGADK 166
Cdd:COG2761   80 EGLPFDFDRIK-PPNTFDAHRLLKAAELQGKQDALLEALFEAYFTEGRDIGDREVLLDLAAEVGLDAEEFRADLESDEAA 158
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*..
gi 499222169 167 EAVAREHAMAVQGGVTGVPFAIFAGKVAVVGAESPERIAQAIDQALA 213
Cdd:COG2761  159 AAVRADEAEARELGVTGVPTFVFDGKYAVSGAQPYEVFEQALRQALA 205
DSBA pfam01323
DSBA-like thioredoxin domain; This family contains a diverse set of proteins with a ...
8-209 1.56e-54

DSBA-like thioredoxin domain; This family contains a diverse set of proteins with a thioredoxin-like structure pfam00085. This family also includes 2-hydroxychromene-2-carboxylate (HCCA) isomerase enzymes catalyze one step in prokaryotic polyaromatic hydrocarbon (PAH) catabolic pathways. This family also contains members with functions other than HCCA isomerization, such as Kappa family GSTs, whose similarity to HCCA isomerases was not previously recognized. The sequence Swiss:O07298 has been annotated as a dioxygenase but is almost certainly an HCCA isomerase enzyme. Similarly, the sequence Swiss:Q9ZI67 has been annotated as a dehydrogenase, but is most probably also an HCCA isomerase enzyme. In addition, the Rhizobium leguminosarum Swiss:Q52782 protein has been annotated as a putative glycerol-3-phosphate transfer protein, but is also most likely to be an HCCA isomerase enzyme.


Pssm-ID: 426200 [Multi-domain]  Cd Length: 191  Bit Score: 172.61  E-value: 1.56e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169    8 IIDVVADVVCPWCYLGWRRLKSAIALRPDLKaqLIWRPYQLDPTLPEDGVDRKAymaqkfkDPEKLKAVHTALVEGGAEE 87
Cdd:pfam01323   1 TVDEFFDFLCPFCYLAKERLEKLAARYGDVK--VVYRPFPLAGAKKIGNVGPSN-------LPVKLKYMMADLERWAALY 71
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169   88 GIVFNFEAIALSpNTNAAHRLIRWALTAGVQDHVVEALFKAYFEQGLDIGDPIVLGDIAEAAGMERLVVLQLLSEGADKE 167
Cdd:pfam01323  72 GIPLRFPANFLG-NSTRANRLALAAGAEGLAEKVVRELFNALWGEGAAITDDSVLREVAEKAGLDAEEFDEFLDSPAVKE 150
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 499222169  168 AVAREHAMAVQGGVTGVPFAIFAGKvAVVGAESPERIAQAID 209
Cdd:pfam01323 151 AVRENTAAAISLGVFGVPTFVVGGK-MVFGADRLDTLADALA 191
DsbA_HCCA_Iso cd03022
DsbA family, 2-hydroxychromene-2-carboxylate (HCCA) isomerase subfamily; HCCA isomerase is a ...
14-191 2.15e-14

DsbA family, 2-hydroxychromene-2-carboxylate (HCCA) isomerase subfamily; HCCA isomerase is a glutathione (GSH) dependent enzyme involved in the naphthalene catabolic pathway. It converts HCCA, a hemiketal formed spontaneously after ring cleavage of 1,2-dihydroxynapthalene by a dioxygenase, into cis-o-hydroxybenzylidenepyruvate (cHBPA). This is the fourth reaction in a six-step pathway that converts napthalene into salicylate. HCCA isomerase is unique to bacteria that degrade polycyclic aromatic compounds. It is closely related to the eukaryotic protein, GSH transferase kappa (GSTK).


Pssm-ID: 239320 [Multi-domain]  Cd Length: 192  Bit Score: 68.42  E-value: 2.15e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169  14 DVVCPWCYLGWRRLKsaiALRPDLKAQLIWRPYQLDPTLPEDG----VDRkaymaqkfkDPEKLKAVHTALVEGGAEEGI 89
Cdd:cd03022    6 DFSSPYSYLAHERLP---ALAARHGATVRYRPILLGGVFKATGnvppANR---------PPAKGRYRLRDLERWARRYGI 73
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169  90 VFNFEAiALSPNTNAAHRLIRWALTAGVQ-DHVVEALFKAYFEQGLDIGDPIVLGDIAEAAGMERLVVLQLLSEGADKEA 168
Cdd:cd03022   74 PLRFPP-RFPPNTLRAMRAALAAQAEGDAaEAFARAVFRALWGEGLDIADPAVLAAVAAAAGLDADELLAAADDPAVKAA 152
                        170       180
                 ....*....|....*....|...
gi 499222169 169 VAREHAMAVQGGVTGVPFAIFAG 191
Cdd:cd03022  153 LRANTEEAIARGVFGVPTFVVDG 175
DsbA_family cd02972
DsbA family; consists of DsbA and DsbA-like proteins, including DsbC, DsbG, glutathione (GSH) ...
9-80 1.11e-09

DsbA family; consists of DsbA and DsbA-like proteins, including DsbC, DsbG, glutathione (GSH) S-transferase kappa (GSTK), 2-hydroxychromene-2-carboxylate (HCCA) isomerase, an oxidoreductase (FrnE) presumed to be involved in frenolicin biosynthesis, a 27-kDa outer membrane protein, and similar proteins. Members of this family contain a redox active CXXC motif (except GSTK and HCCA isomerase) imbedded in a TRX fold, and an alpha helical insert of about 75 residues (shorter in DsbC and DsbG) relative to TRX. DsbA is involved in the oxidative protein folding pathway in prokaryotes, catalyzing disulfide bond formation of proteins secreted into the bacterial periplasm. DsbC and DsbG function as protein disulfide isomerases and chaperones to correct non-native disulfide bonds formed by DsbA and prevent aggregation of incorrectly folded proteins.


Pssm-ID: 239270 [Multi-domain]  Cd Length: 98  Bit Score: 53.56  E-value: 1.11e-09
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 499222169   9 IDVVADVVCPWCYLGWRRLKSAIALRPDlKAQLIWRPYQLDPTLPEDGVdRKAYMAQKFKDPEKLKAVHTAL 80
Cdd:cd02972    1 IVEFFDPLCPYCYLFEPELEKLLYADDG-GVRVVYRPFPLLGGMPPNSL-AAARAALAAAAQGKFEALHEAL 70
COG3531 COG3531
Predicted protein-disulfide isomerase, contains CxxC motif [Posttranslational modification, ...
12-211 3.82e-08

Predicted protein-disulfide isomerase, contains CxxC motif [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 442753 [Multi-domain]  Cd Length: 206  Bit Score: 51.40  E-value: 3.82e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169  12 VADVVCPWCYlG----WRRLKSAiaLRPDLKAQLI---WRPYQLDPTLPEDgvdRKAYMAQKFKDPEKLKAV--HTALVE 82
Cdd:COG3531    7 IYDPLCGWCY-GfapvIEALAEA--LGDRLDVELLsggLFPGSNRRPMDPE---MRAYIQPHWQRIAQLTGQpfGEAYND 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169  83 GGAEEGIVFNFE--AIALSpntnAAHRLirwalTAGVQDHVVEALFKAYFEQGLDIGDPIVLGDIAEAAGMERLVVLQLL 160
Cdd:COG3531   81 LLRDGTFVLDSEpaCRAVL----AAREL-----APERELAMLHAIQRAFYVEGRDISDPEVLAELAAELGLDAEAFAAAL 151
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 499222169 161 SEGADKEAVAREHAMAVQGGVTGVP--FAIFAGKVAVVGAE--SPERIAQAIDQA 211
Cdd:COG3531  152 ASEETRQHIQQEFALARQLGVQGFPtlVLEQGGQLYLLPRGygDPEALLAALEQL 206
DsbG COG1651
Protein thiol-disulfide isomerase DsbC [Posttranslational modification, protein turnover, ...
104-212 1.46e-07

Protein thiol-disulfide isomerase DsbC [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441257 [Multi-domain]  Cd Length: 152  Bit Score: 49.23  E-value: 1.46e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499222169 104 AAHRLIRWALTAGVQDHVvEALFKAYFEQGLDIGDPIVLgDIAEAAGMERLVVLQLLSEGADKEAVAREHAMAVQGGVTG 183
Cdd:COG1651   47 DSLRAARAALCAADQGKF-WAFHDALFANQPALTDDDLR-EIAKEAGLDAAKFDACLNSGAVAAKVEADTALAQALGVTG 124
                         90       100
                 ....*....|....*....|....*....
gi 499222169 184 VPFAIFAGKVaVVGAESPERIAQAIDQAL 212
Cdd:COG1651  125 TPTFVVNGKL-VSGAVPYEELEAALDAAL 152
DsbA_FrnE_like cd03025
DsbA family, FrnE-like subfamily; composed of uncharacterized proteins containing a CXXC motif ...
123-189 5.15e-04

DsbA family, FrnE-like subfamily; composed of uncharacterized proteins containing a CXXC motif with similarity to DsbA and FrnE. FrnE is presumed to be a thiol oxidoreductase involved in polyketide biosynthesis, specifically in the production of the aromatic antibiotics frenolicin and nanaomycins.


Pssm-ID: 239323 [Multi-domain]  Cd Length: 193  Bit Score: 39.61  E-value: 5.15e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 499222169 123 EALFKAYFEQGLDIGDPIVLGDIAEAAGMERLVVLQLLSEGADKEAVAREHAMAVQGGVTGVPFAIF 189
Cdd:cd03025  109 KAIQRAHYVEGRDLADTEVLRELAIELGLDVEEFLEDFQSDEAKQAIQEDQKLARELGINGFPTLVL 175
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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