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Conserved domains on  [gi|499254881|ref|WP_010952421|]
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MULTISPECIES: molybdopterin synthase catalytic subunit MoaE [Pseudomonas]

Protein Classification

molybdenum cofactor biosynthesis protein MoaE( domain architecture ID 10001056)

molybdenum cofactor biosynthesis protein MoaE serves as the catalytic subunit of the molybdopterin synthase complex, which catalyzes the conversion of precursor Z into molybdopterin

CATH:  3.90.1170.40
EC:  2.8.1.12
Gene Ontology:  GO:0006777|GO:0030366
PubMed:  35744859
SCOP:  4001136

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MoaE COG0314
Molybdopterin synthase catalytic subunit MoaE [Coenzyme transport and metabolism]; ...
 1-142 6.32e-65

Molybdopterin synthase catalytic subunit MoaE [Coenzyme transport and metabolism]; Molybdopterin synthase catalytic subunit MoaE is part of the Pathway/BioSystem: Molybdopterin biosynthesis


:

Pssm-ID: 440083 [Multi-domain]  Cd Length: 143  Bit Score: 194.24  E-value: 6.32e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499254881   1 MAVRVQEGAFDPGAETNAMHAANVGVGAVVGFVGYVRDFNDGREVAGMFLEHYPGMTEKALAKIVVEAEQRWPLLKVEVL 80
Cdd:COG0314    2 MRVRVTEEPFDLAAELAALRASDPEAGAVVTFVGTVRDHNDGRRVTALEYEAYPPMAEKELAEIAEEAAERWGLLDVAVI 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 499254881  81 HRIGALEPGEPIVFVGVASAHRQAAFDACNFIMDYLKTRAPFWKKENTQEGPRWVEGRQSDQ 142
Cdd:COG0314   82 HRVGRLEPGEPIVLVAVSSAHRKEAFEACRFLIDRLKTRAPIWKKEIFEDGEEWVEARSSDD 143
 
Name Accession Description Interval E-value
MoaE COG0314
Molybdopterin synthase catalytic subunit MoaE [Coenzyme transport and metabolism]; ...
 1-142 6.32e-65

Molybdopterin synthase catalytic subunit MoaE [Coenzyme transport and metabolism]; Molybdopterin synthase catalytic subunit MoaE is part of the Pathway/BioSystem: Molybdopterin biosynthesis


Pssm-ID: 440083 [Multi-domain]  Cd Length: 143  Bit Score: 194.24  E-value: 6.32e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499254881   1 MAVRVQEGAFDPGAETNAMHAANVGVGAVVGFVGYVRDFNDGREVAGMFLEHYPGMTEKALAKIVVEAEQRWPLLKVEVL 80
Cdd:COG0314    2 MRVRVTEEPFDLAAELAALRASDPEAGAVVTFVGTVRDHNDGRRVTALEYEAYPPMAEKELAEIAEEAAERWGLLDVAVI 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 499254881  81 HRIGALEPGEPIVFVGVASAHRQAAFDACNFIMDYLKTRAPFWKKENTQEGPRWVEGRQSDQ 142
Cdd:COG0314   82 HRVGRLEPGEPIVLVAVSSAHRKEAFEACRFLIDRLKTRAPIWKKEIFEDGEEWVEARSSDD 143
moaE PRK10678
molybdopterin synthase catalytic subunit MoaE;
37-148 1.64e-62

molybdopterin synthase catalytic subunit MoaE;


Pssm-ID: 182642  Cd Length: 150  Bit Score: 188.41  E-value: 1.64e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499254881  37 RDFNDGREVAGMFLEHYPGMTEKALAKIVVEAEQRWPLLKVEVLHRIGALEPGEPIVFVGVASAHRQAAFDACNFIMDYL 116
Cdd:PRK10678  39 RNHNLGDSVKALTLEHYPGMTEKALAEIVDEARSRWPLGRVTVIHRVGELWPGDEIVFVGVTSAHRSSAFEAGQFIMDYL 118

                         90       100       110
                 ....*....|....*....|....*....|..
gi 499254881 117 KTRAPFWKKENTQEGPRWVEGRQSDQDAAGRW 148
Cdd:PRK10678 119 KTRAPFWKREATPEGDRWVEARDSDQQAAKRW 150
MoaE cd00756
MoaE family. Members of this family are involved in biosynthesis of the molybdenum cofactor ...
 37-135 1.78e-52

MoaE family. Members of this family are involved in biosynthesis of the molybdenum cofactor (Moco), an essential cofactor for a diverse group of redox enzymes. Moco biosynthesis is an evolutionarily conserved pathway present in eubacteria, archaea and eukaryotes. Moco contains a tricyclic pyranopterin, termed molybdopterin (MPT), which carries the cis-dithiolene group responsible for molybdenum ligation. This dithiolene group is generated by MPT synthase in the second major step in Moco biosynthesis. MPT synthase is a heterotetramer consisting of two large (MoaE) and two small (MoaD) subunits.


Pssm-ID: 238385 [Multi-domain]  Cd Length: 124  Bit Score: 162.30  E-value: 1.78e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499254881  37 RDFNDGREVAGMFLEHYPGMTEKALAKIVVEAEQRWPLLKVEVLHRIGALEPGEPIVFVGVASAHRQAAFDACNFIMDYL 116
Cdd:cd00756   26 RDHDEGKGVEALEYEAYPPMAEKELEEIAEEARERWGLLRVAIIHRVGRLPPGEAIVLVAVSSPHRKEAFEACEFLIDRL 105

                         90
                 ....*....|....*....
gi 499254881 117 KTRAPFWKKENTQEGPRWV 135
Cdd:cd00756  106 KHRAPIWKKEIFEGGEEWV 124
MoaE pfam02391
MoaE protein; This family contains the MoaE protein that is involved in biosynthesis of ...
 37-119 1.82e-43

MoaE protein; This family contains the MoaE protein that is involved in biosynthesis of molybdopterin. Molybdopterin, the universal component of the pterin molybdenum cofactors, contains a dithiolene group serving to bind Mo. Addition of the dithiolene sulfurs to a molybdopterin precursor requires the activity of the converting factor. Converting factor contains the MoaE and MoaD proteins.


Pssm-ID: 460546  Cd Length: 113  Bit Score: 139.12  E-value: 1.82e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499254881   37 RDFNDGREVAGMFLEHYPGMTEKALAKIVVEAEQRWPLLKVEVLHRIGALEPGEPIVFVGVASAHRQAAFDACNFIMDYL 116
Cdd:pfam02391  31 RDHFDGKKVTALEYEAYPPMAEKELAEIAEEARERWPLLDVAIVHRVGRLPVGEAIVLVAVSSPHRAEAFEACEYLIDEL 110


                  ...
gi 499254881  117 KTR 119
Cdd:pfam02391 111 KAR 113
 
Name Accession Description Interval E-value
MoaE COG0314
Molybdopterin synthase catalytic subunit MoaE [Coenzyme transport and metabolism]; ...
 1-142 6.32e-65

Molybdopterin synthase catalytic subunit MoaE [Coenzyme transport and metabolism]; Molybdopterin synthase catalytic subunit MoaE is part of the Pathway/BioSystem: Molybdopterin biosynthesis


Pssm-ID: 440083 [Multi-domain]  Cd Length: 143  Bit Score: 194.24  E-value: 6.32e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499254881   1 MAVRVQEGAFDPGAETNAMHAANVGVGAVVGFVGYVRDFNDGREVAGMFLEHYPGMTEKALAKIVVEAEQRWPLLKVEVL 80
Cdd:COG0314    2 MRVRVTEEPFDLAAELAALRASDPEAGAVVTFVGTVRDHNDGRRVTALEYEAYPPMAEKELAEIAEEAAERWGLLDVAVI 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 499254881  81 HRIGALEPGEPIVFVGVASAHRQAAFDACNFIMDYLKTRAPFWKKENTQEGPRWVEGRQSDQ 142
Cdd:COG0314   82 HRVGRLEPGEPIVLVAVSSAHRKEAFEACRFLIDRLKTRAPIWKKEIFEDGEEWVEARSSDD 143
moaE PRK10678
molybdopterin synthase catalytic subunit MoaE;
37-148 1.64e-62

molybdopterin synthase catalytic subunit MoaE;


Pssm-ID: 182642  Cd Length: 150  Bit Score: 188.41  E-value: 1.64e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499254881  37 RDFNDGREVAGMFLEHYPGMTEKALAKIVVEAEQRWPLLKVEVLHRIGALEPGEPIVFVGVASAHRQAAFDACNFIMDYL 116
Cdd:PRK10678  39 RNHNLGDSVKALTLEHYPGMTEKALAEIVDEARSRWPLGRVTVIHRVGELWPGDEIVFVGVTSAHRSSAFEAGQFIMDYL 118

                         90       100       110
                 ....*....|....*....|....*....|..
gi 499254881 117 KTRAPFWKKENTQEGPRWVEGRQSDQDAAGRW 148
Cdd:PRK10678 119 KTRAPFWKREATPEGDRWVEARDSDQQAAKRW 150
MoaE cd00756
MoaE family. Members of this family are involved in biosynthesis of the molybdenum cofactor ...
 37-135 1.78e-52

MoaE family. Members of this family are involved in biosynthesis of the molybdenum cofactor (Moco), an essential cofactor for a diverse group of redox enzymes. Moco biosynthesis is an evolutionarily conserved pathway present in eubacteria, archaea and eukaryotes. Moco contains a tricyclic pyranopterin, termed molybdopterin (MPT), which carries the cis-dithiolene group responsible for molybdenum ligation. This dithiolene group is generated by MPT synthase in the second major step in Moco biosynthesis. MPT synthase is a heterotetramer consisting of two large (MoaE) and two small (MoaD) subunits.


Pssm-ID: 238385 [Multi-domain]  Cd Length: 124  Bit Score: 162.30  E-value: 1.78e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499254881  37 RDFNDGREVAGMFLEHYPGMTEKALAKIVVEAEQRWPLLKVEVLHRIGALEPGEPIVFVGVASAHRQAAFDACNFIMDYL 116
Cdd:cd00756   26 RDHDEGKGVEALEYEAYPPMAEKELEEIAEEARERWGLLRVAIIHRVGRLPPGEAIVLVAVSSPHRKEAFEACEFLIDRL 105

                         90
                 ....*....|....*....
gi 499254881 117 KTRAPFWKKENTQEGPRWV 135
Cdd:cd00756  106 KHRAPIWKKEIFEGGEEWV 124
MoaE pfam02391
MoaE protein; This family contains the MoaE protein that is involved in biosynthesis of ...
 37-119 1.82e-43

MoaE protein; This family contains the MoaE protein that is involved in biosynthesis of molybdopterin. Molybdopterin, the universal component of the pterin molybdenum cofactors, contains a dithiolene group serving to bind Mo. Addition of the dithiolene sulfurs to a molybdopterin precursor requires the activity of the converting factor. Converting factor contains the MoaE and MoaD proteins.


Pssm-ID: 460546  Cd Length: 113  Bit Score: 139.12  E-value: 1.82e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499254881   37 RDFNDGREVAGMFLEHYPGMTEKALAKIVVEAEQRWPLLKVEVLHRIGALEPGEPIVFVGVASAHRQAAFDACNFIMDYL 116
Cdd:pfam02391  31 RDHFDGKKVTALEYEAYPPMAEKELAEIAEEARERWPLLDVAIVHRVGRLPVGEAIVLVAVSSPHRAEAFEACEYLIDEL 110


                  ...
gi 499254881  117 KTR 119
Cdd:pfam02391 111 KAR 113
PLN02390 PLN02390
molybdopterin synthase catalytic subunit
 37-126 2.02e-24

molybdopterin synthase catalytic subunit


Pssm-ID: 178014  Cd Length: 111  Bit Score: 90.52  E-value: 2.02e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499254881  37 RDFNDGREVAGMFLEHYPGMTEKALAKIVVEAEQRWPLLKVEVLHRIGALEPGEPIVFVGVASAHRQAAFDACNFIMDYL 116
Cdd:PLN02390  15 RDTFEGKTVLELRYEAYVPMALRELRKICDEARSRWSLHKIAVAHRLGPVPVGETSVFVAVSSVHRADALDACKFLIDEL 94

                         90
                 ....*....|
gi 499254881 117 KTRAPFWKKE 126
Cdd:PLN02390  95 KASVPIWKKE 104
PRK14493 PRK14493
putative bifunctional molybdopterin-guanine dinucleotide biosynthesis protein MobB/MoaE; ...
 51-138 3.67e-18

putative bifunctional molybdopterin-guanine dinucleotide biosynthesis protein MobB/MoaE; Provisional


Pssm-ID: 237730 [Multi-domain]  Cd Length: 274  Bit Score: 78.12  E-value: 3.67e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 499254881  51 EHYPGMTEKALAKIVVEAEQRWPLLKVEVLHRIGALEPGEPIVFVGVASAHRQAAFDACNFIMDYLKTRAPFWKKENTQE 130
Cdd:PRK14493 186 EKYDGVADERMAAIREELKQRDGVFEVLLHHRTGVIEAGEDIVFVVVLAGHRQEAFRAVSDGIDRLKDEVPIFKKEVTVD 265


                 ....*...
gi 499254881 131 GPRWVEGR 138
Cdd:PRK14493 266 EEFWVHDR 273
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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