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Conserved domains on  [gi|501229700|ref|WP_012272718|]
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MULTISPECIES: glycine cleavage system protein GcvH [Pseudomonas]

Protein Classification

glycine cleavage system protein H( domain architecture ID 10793794)

glycine cleavage system protein H plays a role in the degradation of glycine by shuttling the methylamine group of glycine from P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase).

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK13380 PRK13380
glycine cleavage system protein H; Provisional
 1-125 1.82e-66

glycine cleavage system protein H; Provisional


:

Pssm-ID: 237370  Cd Length: 144  Bit Score: 197.53  E-value: 1.82e-66
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501229700   1 MSTLRFTPE-HEWLRLEATGELTVGITSYAQAALGDVVYVQLPEPGAYGE-GNEVAVLESVKAASNITMPLTGTVVAVNQ 78
Cdd:PRK13380  10 PSELRYTIKnHEWLRLEGDGTVTVGITDYAQTMAGDVVFVRLKELGKKVEkGKPVATLESGKWAGPVPAPLTGEVVEVNE 89

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 501229700  79 ALADDPELVNASPMQDGWFFRIQASNPGDLDSLMDQDGYDRFLAENA 125
Cdd:PRK13380  90 ALEDSPELVNEDPYGEGWFFRFKPANPEELKQLLDGDAADRLLKENI 136
 
Name Accession Description Interval E-value
PRK13380 PRK13380
glycine cleavage system protein H; Provisional
 1-125 1.82e-66

glycine cleavage system protein H; Provisional


Pssm-ID: 237370  Cd Length: 144  Bit Score: 197.53  E-value: 1.82e-66
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501229700   1 MSTLRFTPE-HEWLRLEATGELTVGITSYAQAALGDVVYVQLPEPGAYGE-GNEVAVLESVKAASNITMPLTGTVVAVNQ 78
Cdd:PRK13380  10 PSELRYTIKnHEWLRLEGDGTVTVGITDYAQTMAGDVVFVRLKELGKKVEkGKPVATLESGKWAGPVPAPLTGEVVEVNE 89

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 501229700  79 ALADDPELVNASPMQDGWFFRIQASNPGDLDSLMDQDGYDRFLAENA 125
Cdd:PRK13380  90 ALEDSPELVNEDPYGEGWFFRFKPANPEELKQLLDGDAADRLLKENI 136
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 2-123 1.69e-61

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 184.17  E-value: 1.69e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501229700   2 STLRFTPEHEWLRLEATGELTVGITSYAQAALGDVVYVQLPEPGA-YGEGNEVAVLESVKAASNITMPLTGTVVAVNQAL 80
Cdd:COG0509    5 DDLKYTEDHEWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTeVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNEAL 84

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 501229700  81 ADDPELVNASPMQDGWFFRIQASNPGDLDSLMDQDGYDRFLAE 123
Cdd:COG0509   85 EDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEE 127
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 4-123 9.08e-46

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 144.40  E-value: 9.08e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501229700    4 LRFTPEHEWLRLEATGELTVGITSYAQAALGDVVYVQLPEPGAYGE-GNEVAVLESVKAASNITMPLTGTVVAVNQALAD 82
Cdd:pfam01597   1 LYYTKEHEWVKVEGDGTATVGITDFAQAQLGDIVFVELPEVGTKVKkGESLAAIESVKAASPIYAPVSGEVVEVNEKLED 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 501229700   83 DPELVNASPMQDGWFFRIQASNPGDLDSLMDQDGYDRFLAE 123
Cdd:pfam01597  81 NPGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTLKE 121
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 6-100 8.39e-44

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 138.44  E-value: 8.39e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501229700   6 FTPEHEWLRLEATGELTVGITSYAQAALGDVVYVQLPEPGAY-GEGNEVAVLESVKAASNITMPLTGTVVAVNQALADDP 84
Cdd:cd06848    1 YTKDHEWVKVEGDGIATVGITDYAQDLLGDIVFVELPEVGTEvKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDNP 80

                         90
                 ....*....|....*.
gi 501229700  85 ELVNASPMQDGWFFRI 100
Cdd:cd06848   81 ELINSDPYGEGWLVKI 96
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
 4-123 4.15e-40

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 129.96  E-value: 4.15e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501229700    4 LRFTPEHEWLRLEATGELTVGITSYAQAALGDVVYVQLPEPGAYGE-GNEVAVLESVKAASNITMPLTGTVVAVNQALAD 82
Cdd:TIGR00527   6 LRYSSEHEWVRVEGDKTATVGITKFAQDELGDIVFVELPEVGAEVAaGESLGSVESVKAASDIYAPVDGTVVEVNTALED 85

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 501229700   83 DPELVNASPMQDGWFFRIQASNPGD-LDSLMDQDGYDRFLAE 123
Cdd:TIGR00527  86 SPELVNEDPYGDGWLIKVKLSDGESeVEGLMSAEQYEATLEH 127
 
Name Accession Description Interval E-value
PRK13380 PRK13380
glycine cleavage system protein H; Provisional
 1-125 1.82e-66

glycine cleavage system protein H; Provisional


Pssm-ID: 237370  Cd Length: 144  Bit Score: 197.53  E-value: 1.82e-66
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501229700   1 MSTLRFTPE-HEWLRLEATGELTVGITSYAQAALGDVVYVQLPEPGAYGE-GNEVAVLESVKAASNITMPLTGTVVAVNQ 78
Cdd:PRK13380  10 PSELRYTIKnHEWLRLEGDGTVTVGITDYAQTMAGDVVFVRLKELGKKVEkGKPVATLESGKWAGPVPAPLTGEVVEVNE 89

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 501229700  79 ALADDPELVNASPMQDGWFFRIQASNPGDLDSLMDQDGYDRFLAENA 125
Cdd:PRK13380  90 ALEDSPELVNEDPYGEGWFFRFKPANPEELKQLLDGDAADRLLKENI 136
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 2-123 1.69e-61

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 184.17  E-value: 1.69e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501229700   2 STLRFTPEHEWLRLEATGELTVGITSYAQAALGDVVYVQLPEPGA-YGEGNEVAVLESVKAASNITMPLTGTVVAVNQAL 80
Cdd:COG0509    5 DDLKYTEDHEWVRVEGDGTATVGITDFAQDLLGDIVFVELPEVGTeVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNEAL 84

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 501229700  81 ADDPELVNASPMQDGWFFRIQASNPGDLDSLMDQDGYDRFLAE 123
Cdd:COG0509   85 EDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEE 127
PRK01202 PRK01202
glycine cleavage system protein GcvH;
1-123 2.03e-56

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 171.11  E-value: 2.03e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501229700   1 MSTLRFTPEHEWLRLEAtGELTVGITSYAQAALGDVVYVQLPEPGAY-GEGNEVAVLESVKAASNITMPLTGTVVAVNQA 79
Cdd:PRK01202   5 PADLKYTKSHEWVRVEG-DTATVGITDHAQEQLGDIVFVELPEVGDEvKAGETFGVVESVKAASDIYAPVSGEVVEVNEA 83

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 501229700  80 LADDPELVNASPMQDGWFFRIQASNPGDLDSLMDQDGYDRFLAE 123
Cdd:PRK01202  84 LEDSPELVNEDPYGEGWLFKIKPSDESELDDLLDAEAYEALIEE 127
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 4-123 9.08e-46

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 144.40  E-value: 9.08e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501229700    4 LRFTPEHEWLRLEATGELTVGITSYAQAALGDVVYVQLPEPGAYGE-GNEVAVLESVKAASNITMPLTGTVVAVNQALAD 82
Cdd:pfam01597   1 LYYTKEHEWVKVEGDGTATVGITDFAQAQLGDIVFVELPEVGTKVKkGESLAAIESVKAASPIYAPVSGEVVEVNEKLED 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 501229700   83 DPELVNASPMQDGWFFRIQASNPGDLDSLMDQDGYDRFLAE 123
Cdd:pfam01597  81 NPGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTLKE 121
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 6-100 8.39e-44

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 138.44  E-value: 8.39e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501229700   6 FTPEHEWLRLEATGELTVGITSYAQAALGDVVYVQLPEPGAY-GEGNEVAVLESVKAASNITMPLTGTVVAVNQALADDP 84
Cdd:cd06848    1 YTKDHEWVKVEGDGIATVGITDYAQDLLGDIVFVELPEVGTEvKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDNP 80

                         90
                 ....*....|....*.
gi 501229700  85 ELVNASPMQDGWFFRI 100
Cdd:cd06848   81 ELINSDPYGEGWLVKI 96
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
 4-123 4.15e-40

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 129.96  E-value: 4.15e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501229700    4 LRFTPEHEWLRLEATGELTVGITSYAQAALGDVVYVQLPEPGAYGE-GNEVAVLESVKAASNITMPLTGTVVAVNQALAD 82
Cdd:TIGR00527   6 LRYSSEHEWVRVEGDKTATVGITKFAQDELGDIVFVELPEVGAEVAaGESLGSVESVKAASDIYAPVDGTVVEVNTALED 85

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 501229700   83 DPELVNASPMQDGWFFRIQASNPGD-LDSLMDQDGYDRFLAE 123
Cdd:TIGR00527  86 SPELVNEDPYGDGWLIKVKLSDGESeVEGLMSAEQYEATLEH 127
PRK00624 PRK00624
glycine cleavage system protein H; Provisional
 10-111 7.70e-17

glycine cleavage system protein H; Provisional


Pssm-ID: 167014  Cd Length: 114  Bit Score: 70.63  E-value: 7.70e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501229700  10 HEWLRLEATGELTVGITSYAQAALGDVVYVQLPEPGAY-GEGNEVAVLESVKAASNITMPLTGTVVAVNQALADDPELVN 88
Cdd:PRK00624   8 HVWIEPIHSRIVRLGLTSKMQENLGNILHIDLPSVGSFcKEGEVLVILESSKSAIEVLSPVSGEVIEVNTALEDDIQPIN 87

                         90       100
                 ....*....|....*....|...
gi 501229700  89 ASPMQDGWFFRIQASNPGDLDSL 111
Cdd:PRK00624  88 NAPESEGWFVVVQLDEDFDSENL 110
Biotinyl_lipoyl_domains cd06663
Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the ...
 22-100 4.10e-08

Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the dihydrolipoyl acyltransferase component (E2) of 2-oxo acid dehydrogenases, and the H-protein of the glycine cleavage system (GCS). These domains transport CO2, acyl, or methylamine, respectively, between components of the complex/protein via a biotinyl or lipoyl group, which is covalently attached to a highly conserved lysine residue.


Pssm-ID: 133456 [Multi-domain]  Cd Length: 73  Bit Score: 47.05  E-value: 4.10e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501229700  22 TVGITSYAQAaLGDVVYVQ-LPEPG-AYGEGNEVAVLESVKAASNITMPLTGTVVAVNQALADDPELvnaspmqDGWFFR 99
Cdd:cd06663    1 TILIPDLAQH-LGDGTVVKwLKKVGdKVKKGDVLAEIEAMKATSDVEAPKSGTVKKVLVKEGTKVEG-------DTPLVK 72


                 .
gi 501229700 100 I 100
Cdd:cd06663   73 I 73
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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