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Conserved domains on  [gi|501405573|ref|WP_012437139|]
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MULTISPECIES: nuclear transport factor 2 family protein [Xanthomonas]

Protein Classification

nuclear transport factor 2 family protein( domain architecture ID 10020202)

nuclear transport factor 2 (NTF2) family protein with a SnoaL-like polyketide cyclase domain

CATH:  3.10.450.50
SCOP:  3000472|4001306

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TIGR02096 TIGR02096
conserved hypothetical protein, steroid delta-isomerase-related; This family of proteins about ...
 10-138 4.00e-55

conserved hypothetical protein, steroid delta-isomerase-related; This family of proteins about 135 amino acids in length largely restricted to the Proteobacteria. This family and a delta5-3-ketosteroid isomerase from Pseudomonas testosteroni appear homologous, especially toward their respective N-termini. Members, therefore, probably are enzymes.


:

Pssm-ID: 273970  Cd Length: 129  Bit Score: 168.71  E-value: 4.00e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501405573   10 TGLVQAYYEAFNRGDWDAMLAFLAEDVAHDLNQGPREIG-RAAFASFLQRMNDSYREQLRDIVVTANDEGTRVGAEYVVH 88
Cdd:TIGR02096   1 TELAQHWIEAFNRGDMDAVLALLAEDVLYDDNQGGRVLGgKAQLARFLAPYRTAFPDLLVDVVVCRNDEGVRVAAEWTVH 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 501405573   89 GVYHTTDEGLPdANGQTYVLPGGAFFDVRDGQITRVTNYYNLQEWIAQVS 138
Cdd:TIGR02096  81 GTYRTAFLGLP-ASGKTYSIRGVTFFVFDDGKIKRETTYYNLATFLRQLG 129
 
Name Accession Description Interval E-value
TIGR02096 TIGR02096
conserved hypothetical protein, steroid delta-isomerase-related; This family of proteins about ...
 10-138 4.00e-55

conserved hypothetical protein, steroid delta-isomerase-related; This family of proteins about 135 amino acids in length largely restricted to the Proteobacteria. This family and a delta5-3-ketosteroid isomerase from Pseudomonas testosteroni appear homologous, especially toward their respective N-termini. Members, therefore, probably are enzymes.


Pssm-ID: 273970  Cd Length: 129  Bit Score: 168.71  E-value: 4.00e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501405573   10 TGLVQAYYEAFNRGDWDAMLAFLAEDVAHDLNQGPREIG-RAAFASFLQRMNDSYREQLRDIVVTANDEGTRVGAEYVVH 88
Cdd:TIGR02096   1 TELAQHWIEAFNRGDMDAVLALLAEDVLYDDNQGGRVLGgKAQLARFLAPYRTAFPDLLVDVVVCRNDEGVRVAAEWTVH 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 501405573   89 GVYHTTDEGLPdANGQTYVLPGGAFFDVRDGQITRVTNYYNLQEWIAQVS 138
Cdd:TIGR02096  81 GTYRTAFLGLP-ASGKTYSIRGVTFFVFDDGKIKRETTYYNLATFLRQLG 129
YesE COG3631
Ketosteroid isomerase-related protein [General function prediction only];
12-139 5.94e-29

Ketosteroid isomerase-related protein [General function prediction only];


Pssm-ID: 442849  Cd Length: 126  Bit Score: 102.31  E-value: 5.94e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501405573  12 LVQAYYEAFNRGDWDAMLAFLAEDVAHDL---NQGPREIGRAAFASFLQRMNDSYREQLRDIV-VTANdeGTRVGAEYVV 87
Cdd:COG3631    5 LVRRFYAAFNAGDVDALLALLAEDVVWEDpggPGAGTYRGKEAVRAFFARLAAAFEDLRFEVRrVVAD--GDRVVVEGTV 82

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 501405573  88 HGVYHttdeglpdANGQTYVLPGGAFFDVRDGQITRVTNYYNLQEWIAQVSR 139
Cdd:COG3631   83 RGTVL--------ATGKPYENRYADVFTVRDGKIVRVREYFDTAALAAALGA 126
SnoaL_2 pfam12680
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 13-122 5.62e-18

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 432716 [Multi-domain]  Cd Length: 100  Bit Score: 73.47  E-value: 5.62e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501405573   13 VQAYYEAFNRGDWDAMLAFLAEDVAHDLNQGPREiGRAAFASFLQRMndsyREQLRDIVVTAND---EGTRVGAEYVVHG 89
Cdd:pfam12680   1 VRRFYEALNAGDLDALAALFAPDAVFHDPGGPLR-GRDAIRAFFAAL----FAAFPDLRFEIHDviaDGDRVAVRWTVTG 75

                          90       100       110
                  ....*....|....*....|....*....|...
gi 501405573   90 VyhttdeglPDANGQTYVLPGGAFFDVRDGQIT 122
Cdd:pfam12680  76 T--------IPPTGRGVRVRGVDVFRFRDGKIV 100
NTF2_like cd00531
Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 ...
 9-129 5.45e-05

Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 family, Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases, and the beta subunit of Ring hydroxylating dioxygenases. This family is a classic example of divergent evolution wherein the proteins have many common structural details but diverge greatly in their function. For example, nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid, by intramolecular transfer of the C4-beta proton to the C6-beta position. While the function of the beta sub-unit of the Ring hydroxylating dioxygenases is not known, Scytalone Dehydratases catalyzes two reactions in the biosynthetic pathway that produces fungal melanin. Members of the NTF2-like superfamily are widely distributed among bacteria, archaea and eukaryotes.


Pssm-ID: 238296 [Multi-domain]  Cd Length: 124  Bit Score: 40.19  E-value: 5.45e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501405573   9 ATGLVQAYYEAFNRGDWDAMLAFLAEDVAHDLNQGPREI----GRAAFASFLQRMNDSYREQLR-DIVVTANDEGTRVGA 83
Cdd:cd00531    1 AEQFLYRYARLLDAGDREWLALLYADDAYFEPPGGDGLIypddGREAIEDRVRRLPFGPSRTRHlVSNVDVQPGDDGEGV 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 501405573  84 EYVVHGVYHTTDEglpdanGQTYVLPGGAFFDVR----DGQITRVTNYYN 129
Cdd:cd00531   81 VVSVFGVLRTRGD------GEQDVFAGGQTFVLRpqggGGKIANRRFRLD 124
PRK08241 PRK08241
RNA polymerase subunit sigma-70;
12-129 7.12e-03

RNA polymerase subunit sigma-70;


Pssm-ID: 236196 [Multi-domain]  Cd Length: 339  Bit Score: 35.30  E-value: 7.12e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501405573  12 LVQAYYEAFNRGDWDAMLAFLAEDVAHDLNQGPREI-GRAAFASFLQRMNDSYR-EQLRDIVVTANdeGTRVGAEYvvhg 89
Cdd:PRK08241 219 LLARYVAAFEAYDVDALVALLTEDATWSMPPFPLWYrGRDAIAAFLAGQCPGAGcGGSRLVPTRAN--GQPAFAQY---- 292

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 501405573  90 vyhttdegLPDANGQTYVLPGGAFFDVRDGQITRVTNYYN 129
Cdd:PRK08241 293 --------MRDPDGGGHRPWALHVLELRGGRIAHVTSFLD 324
 
Name Accession Description Interval E-value
TIGR02096 TIGR02096
conserved hypothetical protein, steroid delta-isomerase-related; This family of proteins about ...
 10-138 4.00e-55

conserved hypothetical protein, steroid delta-isomerase-related; This family of proteins about 135 amino acids in length largely restricted to the Proteobacteria. This family and a delta5-3-ketosteroid isomerase from Pseudomonas testosteroni appear homologous, especially toward their respective N-termini. Members, therefore, probably are enzymes.


Pssm-ID: 273970  Cd Length: 129  Bit Score: 168.71  E-value: 4.00e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501405573   10 TGLVQAYYEAFNRGDWDAMLAFLAEDVAHDLNQGPREIG-RAAFASFLQRMNDSYREQLRDIVVTANDEGTRVGAEYVVH 88
Cdd:TIGR02096   1 TELAQHWIEAFNRGDMDAVLALLAEDVLYDDNQGGRVLGgKAQLARFLAPYRTAFPDLLVDVVVCRNDEGVRVAAEWTVH 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 501405573   89 GVYHTTDEGLPdANGQTYVLPGGAFFDVRDGQITRVTNYYNLQEWIAQVS 138
Cdd:TIGR02096  81 GTYRTAFLGLP-ASGKTYSIRGVTFFVFDDGKIKRETTYYNLATFLRQLG 129
YesE COG3631
Ketosteroid isomerase-related protein [General function prediction only];
12-139 5.94e-29

Ketosteroid isomerase-related protein [General function prediction only];


Pssm-ID: 442849  Cd Length: 126  Bit Score: 102.31  E-value: 5.94e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501405573  12 LVQAYYEAFNRGDWDAMLAFLAEDVAHDL---NQGPREIGRAAFASFLQRMNDSYREQLRDIV-VTANdeGTRVGAEYVV 87
Cdd:COG3631    5 LVRRFYAAFNAGDVDALLALLAEDVVWEDpggPGAGTYRGKEAVRAFFARLAAAFEDLRFEVRrVVAD--GDRVVVEGTV 82

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 501405573  88 HGVYHttdeglpdANGQTYVLPGGAFFDVRDGQITRVTNYYNLQEWIAQVSR 139
Cdd:COG3631   83 RGTVL--------ATGKPYENRYADVFTVRDGKIVRVREYFDTAALAAALGA 126
SnoaL_2 pfam12680
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 13-122 5.62e-18

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 432716 [Multi-domain]  Cd Length: 100  Bit Score: 73.47  E-value: 5.62e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501405573   13 VQAYYEAFNRGDWDAMLAFLAEDVAHDLNQGPREiGRAAFASFLQRMndsyREQLRDIVVTAND---EGTRVGAEYVVHG 89
Cdd:pfam12680   1 VRRFYEALNAGDLDALAALFAPDAVFHDPGGPLR-GRDAIRAFFAAL----FAAFPDLRFEIHDviaDGDRVAVRWTVTG 75

                          90       100       110
                  ....*....|....*....|....*....|...
gi 501405573   90 VyhttdeglPDANGQTYVLPGGAFFDVRDGQIT 122
Cdd:pfam12680  76 T--------IPPTGRGVRVRGVDVFRFRDGKIV 100
COG5485 COG5485
Predicted ester cyclase [General function prediction only];
12-136 1.21e-13

Predicted ester cyclase [General function prediction only];


Pssm-ID: 444236 [Multi-domain]  Cd Length: 130  Bit Score: 62.95  E-value: 1.21e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501405573  12 LVQAYYE-AFNRGDWDAMLAFLAEDVAHDLNQGPREIGRAAFASFLQRMndsyREQLRDIVVTAND---EGTRVGAEYVV 87
Cdd:COG5485    1 LVRRFFEeAWNEGDLDALDELVAPDFVYHGPLPGEPRGPEGFRAFLRGL----RAAFPDLRFEIEDliaEGDRVAVRWTF 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 501405573  88 HGVyHTTDEGLPDANGQTYVLPGGAFFDVRDGQITRVTNYYNLQEWIAQ 136
Cdd:COG5485   77 TGT-HTGEFLGIPPTGKRVEFRGIDIYRFEDGKIVEEWVVEDLLGLLQQ 124
SnoaL pfam07366
SnoaL-like polyketide cyclase; This family includes SnoaL a polyketide cyclase involved in ...
 12-136 4.12e-11

SnoaL-like polyketide cyclase; This family includes SnoaL a polyketide cyclase involved in nogalamycin biosynthesis. This family was formerly known as DUF1486. The proteins in this family adopt a distorted alpha-beta barrel fold. Structural data together with site-directed mutagenesis experiments have shown that SnoaL has a different mechanism to that of the classical aldolase for catalysing intramolecular aldol condensation.


Pssm-ID: 399975  Cd Length: 126  Bit Score: 56.54  E-value: 4.12e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501405573   12 LVQAYYEAFNRGDWDAMLAFLAEDVA-HDLNQGPReIGRAAFASFLQRMndsyREQLRDIVVTAND---EGTRVGAEYVV 87
Cdd:pfam07366   3 VLRFYDEVWNTGDLDALDEFVAEDVVdHNPPPGVA-RGLDGFRAFAEML----FRAFPDLRFEVEDlvaEGDKVAARLTF 77

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 501405573   88 HGVYHTTDEGLPdANGQTYVLPGGAFFDVRDGQITRVTNYYNLQEWIAQ 136
Cdd:pfam07366  78 TGTHTGEFLGIP-PTGRKVRFRGIDLYRFEDGKIVEHWDVIDLLGLLRQ 125
YybH COG4319
Ketosteroid isomerase homolog YybH [General function prediction only];
12-77 7.17e-08

Ketosteroid isomerase homolog YybH [General function prediction only];


Pssm-ID: 443460 [Multi-domain]  Cd Length: 131  Bit Score: 47.83  E-value: 7.17e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 501405573  12 LVQAYYEAFNRGDWDAMLAFLAEDVAHDLNQGPREIGRAAFASFLQRM---NDSYREQLRDIVVTANDE 77
Cdd:COG4319   14 LLAAFAEAFNAGDADALAALYAEDAVFFDPGGPPVRGREAIRAAWAAAfaaGPRVTFEVEDVRVLVSGD 82
SnoaL COG4538
Uncharacterized conserved protein, contains SnoaL-like domain [Function unknown];
9-125 2.50e-05

Uncharacterized conserved protein, contains SnoaL-like domain [Function unknown];


Pssm-ID: 443604  Cd Length: 113  Bit Score: 40.65  E-value: 2.50e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501405573   9 ATGLVQAYYEAFNRGDWDAMLAFLAEDV-AHDLNQGPREIGRAAFASFLQRMNDSYRE---QLRDIVVTANdegtrvgae 84
Cdd:COG4538    5 PEDVVQRQLDAYNARDIDAFLATYADDAeVYRFPGTLLAKGREAIRERYAERFANEPDlhaELVSRIVLGN--------- 75

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|.
gi 501405573  85 YVVHgvyHTTDEGLPDaNGQTYVLpggAFFDVRDGQITRVT 125
Cdd:COG4538   76 KVID---HELVTGLFG-GGPVEVV---AIYEVEDGLIARVW 109
NTF2_like cd00531
Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 ...
 9-129 5.45e-05

Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 family, Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases, and the beta subunit of Ring hydroxylating dioxygenases. This family is a classic example of divergent evolution wherein the proteins have many common structural details but diverge greatly in their function. For example, nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid, by intramolecular transfer of the C4-beta proton to the C6-beta position. While the function of the beta sub-unit of the Ring hydroxylating dioxygenases is not known, Scytalone Dehydratases catalyzes two reactions in the biosynthetic pathway that produces fungal melanin. Members of the NTF2-like superfamily are widely distributed among bacteria, archaea and eukaryotes.


Pssm-ID: 238296 [Multi-domain]  Cd Length: 124  Bit Score: 40.19  E-value: 5.45e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501405573   9 ATGLVQAYYEAFNRGDWDAMLAFLAEDVAHDLNQGPREI----GRAAFASFLQRMNDSYREQLR-DIVVTANDEGTRVGA 83
Cdd:cd00531    1 AEQFLYRYARLLDAGDREWLALLYADDAYFEPPGGDGLIypddGREAIEDRVRRLPFGPSRTRHlVSNVDVQPGDDGEGV 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 501405573  84 EYVVHGVYHTTDEglpdanGQTYVLPGGAFFDVR----DGQITRVTNYYN 129
Cdd:cd00531   81 VVSVFGVLRTRGD------GEQDVFAGGQTFVLRpqggGGKIANRRFRLD 124
SnoaL_3 pfam13474
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 12-58 1.00e-04

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 433238  Cd Length: 121  Bit Score: 39.26  E-value: 1.00e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 501405573   12 LVQAYYEAFNRGDWDAMLAFLAEDVAHdLNQGPRE--IGRAAFASFLQR 58
Cdd:pfam13474   4 LNDAWYEAAAEGDFDALFALWSDDISC-IGTGAGElwRGREEFRRSWKQ 51
PRK08241 PRK08241
RNA polymerase subunit sigma-70;
12-129 7.12e-03

RNA polymerase subunit sigma-70;


Pssm-ID: 236196 [Multi-domain]  Cd Length: 339  Bit Score: 35.30  E-value: 7.12e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 501405573  12 LVQAYYEAFNRGDWDAMLAFLAEDVAHDLNQGPREI-GRAAFASFLQRMNDSYR-EQLRDIVVTANdeGTRVGAEYvvhg 89
Cdd:PRK08241 219 LLARYVAAFEAYDVDALVALLTEDATWSMPPFPLWYrGRDAIAAFLAGQCPGAGcGGSRLVPTRAN--GQPAFAQY---- 292

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 501405573  90 vyhttdegLPDANGQTYVLPGGAFFDVRDGQITRVTNYYN 129
Cdd:PRK08241 293 --------MRDPDGGGHRPWALHVLELRGGRIAHVTSFLD 324
ketosteroid_isomerase cd00781
ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond ...
 10-57 8.86e-03

ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond adjacent to carbonyl or a carboxyl group, leading to an enol or a enolate intermediate that is subsequently re-protonated at the same or an adjacent carbon. Ketosteroid isomerases are important members of this class of enzymes which are the most proficient of all enzymes known and have served as a paradigm for enzymatic enolizations since its discovery in 1954. This CD includes members of this class that calalyze the isomerization of various beta,gamma-unsaturated isomers at nearly a diffusion-controlled rate. These enzymes are widely distributed in bacteria.


Pssm-ID: 238404  Cd Length: 122  Bit Score: 33.94  E-value: 8.86e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 501405573  10 TGLVQAYYEAFNRGDWDAMLAFLAED--VAHDLNQGPREiGRAAFASFLQ 57
Cdd:cd00781    6 KAAVQRYVEAVNAGDPEGIVALFADDatVEDPVGSPPRS-GRAAIAAFYA 54
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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