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Conserved domains on  [gi|503864120|ref|WP_014098114|]
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MULTISPECIES: GNAT family N-acetyltransferase [Heyndrickxia]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10456837)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  15581578|10940244|9175471|27367672|26818562
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 16-112 1.82e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


:

Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 51.36  E-value: 1.82e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503864120   16 LLSFMPDEKDLKKLQQTKQLYETEDKWQLFLWKEDDDIVGLIGVVLDGDK---VEIQHISVNPSYRDQGIGKSMVKALKQ 92
Cdd:pfam00583   7 LLSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEppvGEIEGLAVAPEYRGKGIGTALLQALLE 86

                          90       100
                  ....*....|....*....|....*...
gi 503864120   93 MYEGKEV--------VPNKYIVDFFHKC 112
Cdd:pfam00583  87 WARERGCeriflevaADNLAAIALYEKL 114
 
Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 16-112 1.82e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 51.36  E-value: 1.82e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503864120   16 LLSFMPDEKDLKKLQQTKQLYETEDKWQLFLWKEDDDIVGLIGVVLDGDK---VEIQHISVNPSYRDQGIGKSMVKALKQ 92
Cdd:pfam00583   7 LLSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEppvGEIEGLAVAPEYRGKGIGTALLQALLE 86

                          90       100
                  ....*....|....*....|....*...
gi 503864120   93 MYEGKEV--------VPNKYIVDFFHKC 112
Cdd:pfam00583  87 WARERGCeriflevaADNLAAIALYEKL 114
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 22-92 2.46e-08

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 48.45  E-value: 2.46e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 503864120  22 DEKDLKKLQQTKQLYETEDkwQLFLWKEDDDIVGLIGV-VLDGDKVEIQHISVNPSYRDQGIGKSMVKALKQ 92
Cdd:COG1246   10 DVPAILELIRPYALEEEIG--EFWVAEEDGEIVGCAALhPLDEDLAELRSLAVHPDYRGRGIGRRLLEALLA 79
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 44-92 1.07e-07

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 45.34  E-value: 1.07e-07
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 503864120  44 LFLWKEDDDIVGLIGVVLDG---DKVEIQHISVNPSYRDQGIGKSMVKALKQ 92
Cdd:cd04301    1 FLVAEDDGEIVGFASLSPDGsggDTAYIGDLAVLPEYRGKGIGSALLEAAEE 52
PRK07757 PRK07757
N-acetyltransferase;
45-118 1.19e-04

N-acetyltransferase;


Pssm-ID: 236088 [Multi-domain]  Cd Length: 152  Bit Score: 39.02  E-value: 1.19e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503864120  45 FLWKEDDDIVGLIGV-VLDGDKVEIQHISVNPSYRDQGIGKSMVKALkqMYEGKEVVPNK-----YIVDFFHKC----AE 114
Cdd:PRK07757  44 YVAEEEGEIVGCCALhILWEDLAEIRSLAVSEDYRGQGIGRMLVEAC--LEEARELGVKRvfaltYQPEFFEKLgfreVD 121


                 ....
gi 503864120 115 KEDL 118
Cdd:PRK07757 122 KEAL 125
 
Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 16-112 1.82e-09

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 51.36  E-value: 1.82e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503864120   16 LLSFMPDEKDLKKLQQTKQLYETEDKWQLFLWKEDDDIVGLIGVVLDGDK---VEIQHISVNPSYRDQGIGKSMVKALKQ 92
Cdd:pfam00583   7 LLSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIIDDEppvGEIEGLAVAPEYRGKGIGTALLQALLE 86

                          90       100
                  ....*....|....*....|....*...
gi 503864120   93 MYEGKEV--------VPNKYIVDFFHKC 112
Cdd:pfam00583  87 WARERGCeriflevaADNLAAIALYEKL 114
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 22-92 2.46e-08

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 48.45  E-value: 2.46e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 503864120  22 DEKDLKKLQQTKQLYETEDkwQLFLWKEDDDIVGLIGV-VLDGDKVEIQHISVNPSYRDQGIGKSMVKALKQ 92
Cdd:COG1246   10 DVPAILELIRPYALEEEIG--EFWVAEEDGEIVGCAALhPLDEDLAELRSLAVHPDYRGRGIGRRLLEALLA 79
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 44-92 1.07e-07

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 45.34  E-value: 1.07e-07
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 503864120  44 LFLWKEDDDIVGLIGVVLDG---DKVEIQHISVNPSYRDQGIGKSMVKALKQ 92
Cdd:cd04301    1 FLVAEDDGEIVGFASLSPDGsggDTAYIGDLAVLPEYRGKGIGSALLEAAEE 52
Acetyltransf_CG pfam14542
GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both ...
 45-118 1.95e-07

GCN5-related N-acetyl-transferase; This family of GCN5-related N-acetyl-transferases bind both CoA and acetyl-CoA. They are characterized by highly conserved glycine, a cysteine residue in the acetyl-CoA binding site near the acetyl group, their small size compared with other GNATs and a lack of of an obvious substrate-binding site. It is proposed that they transfer an acetyl group from acetyl-CoA to one or more unidentified aliphatic amines via an acetyl (cysteine) enzyme intermediate. The substrate might be another macromolecule.


Pssm-ID: 434030 [Multi-domain]  Cd Length: 79  Bit Score: 45.20  E-value: 1.95e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 503864120   45 FLWKEDDDIVGLIGVVLDGDKVEIQHISVNPSYRDQGIGKSMVKAL----KQmyEGKEVVPN-KYIVDFFHKCAEKEDL 118
Cdd:pfam14542   3 EIRVDGGAEVAFLTYRRGDGVLIITHTEVPPALRGQGIASKLVKAAlddaRE--EGLKIVPLcSYVAAYLEKHPEYADL 79
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 40-99 3.65e-07

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 44.37  E-value: 3.65e-07
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 503864120   40 DKWQLFLWKEDDDIVGLIGVVLDGDKVEIQHIS--VNPSYRDQGIGKSMVKALKQMYEGKEV 99
Cdd:pfam13508   1 PGGRFFVAEDDGKIVGFAALLPLDDEGALAELRlaVHPEYRGQGIGRALLEAAEAAAKEGGI 62
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 21-92 2.59e-06

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 43.50  E-value: 2.59e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 503864120  21 PDEKDLKKLQQT-----KQLYETEDKWQLFLWKEDDDIVGLIGV-VLDGDKVEIQHISVNPSYRDQGIGKSMVKALKQ 92
Cdd:COG0454    8 PEDINFILLIEAldaelKAMEGSLAGAEFIAVDDKGEPIGFAGLrRLDDKVLELKRLYVLPEYRGKGIGKALLEALLE 85
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
34-92 1.68e-05

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 41.22  E-value: 1.68e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 503864120  34 QLYETEDKWQLFLWKEDDDIVGLIG-----VVLDGDKVEIQHISVNPSYRDQGIGKSMVKALKQ 92
Cdd:COG3153   31 RLREDPAAGLSLVAEDDGEIVGHVAlspvdIDGEGPALLLGPLAVDPEYRGQGIGRALMRAALE 94
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 33-111 2.93e-05

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 40.33  E-value: 2.93e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503864120   33 KQLYETEDKWQLFLW--KEDDDIVGLIGVvldGDKVEIQHISVNPSYRDQGIGKSMVKALKQMYEGKEVVPNK------- 103
Cdd:pfam13673  20 EALRERIDQGEYFFFvaFEGGQIVGVIAL---RDRGHISLLFVDPDYQGQGIGKALLEAVEDYAEKDGIKLSEltvnasp 96


                  ....*...
gi 503864120  104 YIVDFFHK 111
Cdd:pfam13673  97 YAVPFYEK 104
COG3981 COG3981
Predicted acetyltransferase [General function prediction only];
13-93 3.05e-05

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443180  Cd Length: 170  Bit Score: 41.05  E-value: 3.05e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503864120  13 AMGLLSFMPDEKDLKKLQQTKQLYETEDKW----QLFLWKEDDDIVGLIGV--VL-DGDKVEIQHI--SVNPSYRDQGIG 83
Cdd:COG3981   30 SGYLVSFEDFEAWLERLLDEEKGEELPEGWvpatTYWLVDEDGRIVGAINLrhELnEFLLRVGGHIgyGVRPSERGKGYA 109

                         90
                 ....*....|
gi 503864120  84 KsmvKALKQM 93
Cdd:COG3981  110 T---EMLRLA 116
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 56-92 1.10e-04

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 38.10  E-value: 1.10e-04
                         10        20        30
                 ....*....|....*....|....*....|....*..
gi 503864120  56 LIGVVLDGDKVEIQHISVNPSYRDQGIGKSMVKALKQ 92
Cdd:COG0456    4 LLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALE 40
PRK07757 PRK07757
N-acetyltransferase;
45-118 1.19e-04

N-acetyltransferase;


Pssm-ID: 236088 [Multi-domain]  Cd Length: 152  Bit Score: 39.02  E-value: 1.19e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 503864120  45 FLWKEDDDIVGLIGV-VLDGDKVEIQHISVNPSYRDQGIGKSMVKALkqMYEGKEVVPNK-----YIVDFFHKC----AE 114
Cdd:PRK07757  44 YVAEEEGEIVGCCALhILWEDLAEIRSLAVSEDYRGQGIGRMLVEAC--LEEARELGVKRvfaltYQPEFFEKLgfreVD 121


                 ....
gi 503864120 115 KEDL 118
Cdd:PRK07757 122 KEAL 125
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 29-90 4.11e-04

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 37.67  E-value: 4.11e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 503864120  29 LQQTKQLYETEDKWQLFLW-KEDDDIVGLIG---VVLDGDKVEIqHISVNPSYRDQGIGKSMVKAL 90
Cdd:COG1670   48 LERLLADWADGGALPFAIEdKEDGELIGVVGlydIDRANRSAEI-GYWLAPAYWGKGYATEALRAL 112
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
45-90 4.90e-03

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 34.39  E-value: 4.90e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 503864120  45 FLWKEDDDIVGLIGVVLDGDK-VEIQHISVNPSYRDQGIGKSMVKAL 90
Cdd:COG2153   37 LLAYDDGELVATARLLPPGDGeAKIGRVAVLPEYRGQGLGRALMEAA 83
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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