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Conserved domains on  [gi|504709958|ref|WP_014897060|]
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PIN domain-containing protein [Burkholderia cepacia]

Protein Classification

PIN domain-containing protein( domain architecture ID 1000090)

PIN (PilT N terminus) domain-containing protein may function as a nuclease

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PIN_SF super family cl28905
PIN (PilT N terminus) domain: Superfamily; The PIN (PilT N terminus) domain belongs to a large ...
 6-136 3.50e-33

PIN (PilT N terminus) domain: Superfamily; The PIN (PilT N terminus) domain belongs to a large nuclease superfamily, and were originally named for their sequence similarity to the N-terminal domain of an annotated pili biogenesis protein, PilT, a domain fusion between a PIN-domain and a PilT ATPase domain. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons. The PIN domain superfamily includes: the FEN-like PIN domain family such as the PIN domains of Flap endonuclease-1 (FEN1), exonuclease-1 (EXO1), Mkt1, Gap Endonuclease 1 (GEN1), and Xeroderma pigmentosum complementation group G (XPG) nuclease, 5'-3' exonucleases of DNA polymerase I and bacteriophage T4- and T5-5' nucleases; the VapC-like PIN domain family which includes toxins of prokaryotic toxin/antitoxin operons FitAB and VapBC, as well as eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1; the LabA-like PIN domain family which includes the PIN domains of Synechococcus elongatus LabA (low-amplitude and bright); the PRORP-Zc3h12a-like PIN domain family which includes the PIN domains of RNase P (PRORP), ribonuclease Zc3h12a; and Bacillus subtilis YacP/Rae1-like PIN domains. It also includes the Mut7-C PIN domain family, which is not represented here as it is a shortened version of the PIN fold and lacks a core strand and helix (H3 and S3). The Mut7-C PIN domain family includes the C-terminus of Caenorhabditis elegans exonuclease Mut-7.


The actual alignment was detected with superfamily member cd18735:

Pssm-ID: 475124  Cd Length: 128  Bit Score: 113.08  E-value: 3.50e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   6 MLDTNICSFIMRERPPVVLSRLQSCvnAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLdGVLSWDTAAVDAT 85
Cdd:cd18735    1 LLDTNICIYIRKQRPPSVLERFAQL--QPGEVAMSVITYGELLYGAEKSQRRAKALEALERLAELI-PVLPLPEEVAEHY 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 504709958  86 AAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDW 136
Cdd:cd18735   78 GEIRADLEKKGTPIGNNDLWIAAHALALGLTLVTNNTREFQRVPGLKVENW 128
 
Name Accession Description Interval E-value
PIN_HiVapC1-like cd18735
VapC-like PIN domain of Haemophilus influenzae VapC1 and related proteins; Haemophilus ...
 6-136 3.50e-33

VapC-like PIN domain of Haemophilus influenzae VapC1 and related proteins; Haemophilus influenzae VapC1 has endonucleolytic activity with RNA, it cleaves initiator tRNA between the anticodon stem and loop, but does not cleave mRNA, rRNA or tmRNA, and has no activity on ssDNA or dsDNA. This subfamily belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. FitB is a toxin of the FitAB TA system. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350302  Cd Length: 128  Bit Score: 113.08  E-value: 3.50e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   6 MLDTNICSFIMRERPPVVLSRLQSCvnAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLdGVLSWDTAAVDAT 85
Cdd:cd18735    1 LLDTNICIYIRKQRPPSVLERFAQL--QPGEVAMSVITYGELLYGAEKSQRRAKALEALERLAELI-PVLPLPEEVAEHY 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 504709958  86 AAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDW 136
Cdd:cd18735   78 GEIRADLEKKGTPIGNNDLWIAAHALALGLTLVTNNTREFQRVPGLKVENW 128
VapC COG1487
Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];
5-136 3.19e-27

Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];


Pssm-ID: 441096  Cd Length: 128  Bit Score: 98.01  E-value: 3.19e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   5 YMLDTNICSFIMRERPPVVLSRLQSCvnAQHRIVVSAITYAEMRFGAvGRKASPKHAELVTAFVARLDgVLSWDTAAVDA 84
Cdd:COG1487    1 YLLDTNVLIDLLRGRPPAVLAWLAEL--EAGELAISAITLAELLYGA-RRSPDAKRRAALEAFLEAFP-VLPFDAEAARR 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 504709958  85 TAAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDW 136
Cdd:COG1487   77 AGELRAALRRAGRPIGLADLLIAATALAHGLTLVTRNTKDFERIPGLKVEPW 128
PRK13725 PRK13725
tRNA(fMet)-specific endonuclease VapC;
5-137 1.01e-26

tRNA(fMet)-specific endonuclease VapC;


Pssm-ID: 184277  Cd Length: 132  Bit Score: 97.02  E-value: 1.01e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   5 YMLDTNICSFIMRERPPVVLSRLQscVNaQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLDgVLSWDTAAVDA 84
Cdd:PRK13725   4 FMLDTNICIFTIKNKPEHVRERFN--LN-TGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLE-VLDYDTAAATH 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 504709958  85 TAAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDWA 137
Cdd:PRK13725  80 TGQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVPGIRIEDWS 132
PIN pfam01850
PIN domain;
5-131 2.27e-04

PIN domain;


Pssm-ID: 426475  Cd Length: 121  Bit Score: 38.54  E-value: 2.27e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958    5 YMLDTNICSFIMRERPPVVLSRLQscVNAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLDGVLSWDTAAVDA 84
Cdd:pfam01850   1 IVLDTSVLIALLRGEPLHEAAREL--LEAAGELVTSAIVLAELLYGLNRRLGLGKAAELVELLLLLSALEVLPIDAELAE 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 504709958   85 TAairADLAARGTPIGANDASIAGHAIAAGAVLVTNNNReFSRVAGL 131
Cdd:pfam01850  79 EA---AELRLKYGKLGPNDALIAATAKEHGAKLITFDED-FARVAKL 121
 
Name Accession Description Interval E-value
PIN_HiVapC1-like cd18735
VapC-like PIN domain of Haemophilus influenzae VapC1 and related proteins; Haemophilus ...
 6-136 3.50e-33

VapC-like PIN domain of Haemophilus influenzae VapC1 and related proteins; Haemophilus influenzae VapC1 has endonucleolytic activity with RNA, it cleaves initiator tRNA between the anticodon stem and loop, but does not cleave mRNA, rRNA or tmRNA, and has no activity on ssDNA or dsDNA. This subfamily belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. FitB is a toxin of the FitAB TA system. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350302  Cd Length: 128  Bit Score: 113.08  E-value: 3.50e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   6 MLDTNICSFIMRERPPVVLSRLQSCvnAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLdGVLSWDTAAVDAT 85
Cdd:cd18735    1 LLDTNICIYIRKQRPPSVLERFAQL--QPGEVAMSVITYGELLYGAEKSQRRAKALEALERLAELI-PVLPLPEEVAEHY 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 504709958  86 AAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDW 136
Cdd:cd18735   78 GEIRADLEKKGTPIGNNDLWIAAHALALGLTLVTNNTREFQRVPGLKVENW 128
PIN_VapC4-5_FitB-like cd09881
VapC-like PIN domain of Mycobacterium tuberculosis VapC4 and VapC5, and Neisseria gonorrhoeae ...
 6-135 5.95e-32

VapC-like PIN domain of Mycobacterium tuberculosis VapC4 and VapC5, and Neisseria gonorrhoeae FitB and related proteins; This family includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This family belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350229  Cd Length: 127  Bit Score: 109.88  E-value: 5.95e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   6 MLDTNICSFIMRERPPVVLSRLQSCvnAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLDgVLSWDTAAVDAT 85
Cdd:cd09881    1 LLDTNICIDLLRGRPPSVLERLEEL--LPEDLAISSITLAELLYGAEKSQRPEKNLAALEAFLSRLE-VLPFDEEAAEIY 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 504709958  86 AAIRADLAARGTPIGAND---ASIAGHAIAagaVLVTNNNREFSRVAGLSLED 135
Cdd:cd09881   78 GEIRAELEKKGKPIGDNDlliAATALAHGL---TLVTNNTKHFERIPGLKVEN 127
PIN_VapC4-5_FitB-like cd18748
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 6-136 4.65e-30

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350315  Cd Length: 128  Bit Score: 105.31  E-value: 4.65e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   6 MLDTNICSFIMRERPPVVLSRLQScvNAQHRIVVSAITYAEMRFGAVgRKASPKHAELVTAFVARLDgVLSWDTAAVDAT 85
Cdd:cd18748    1 LLDTNIISDLIRNPRGKVARRIRE--VGEDAVCTSIIVASELRFGAA-KKGSERLARRVEAVLSRIP-VLPLEPPADEHY 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 504709958  86 AAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDW 136
Cdd:cd18748   77 ARIRADLERQGTPIGPNDLLIAAHALALDLTLVTDNTREFSRVPGLKVENW 127
PIN_VapC4-5_FitB-like cd18745
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 5-137 2.59e-27

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350312  Cd Length: 130  Bit Score: 98.34  E-value: 2.59e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   5 YMLDTNICSFIMRERPPVVLSRLQSCvnAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLDgVLSWDTAAVDA 84
Cdd:cd18745    1 YLLDTNTVIALFRGRGPVLRARLRAA--APSDLALSSIVLYELEYGAAKSARPERNRARLEELLSLIE-VLPFDEEDARA 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 504709958  85 TAAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDWA 137
Cdd:cd18745   78 AAEIRADLERAGTPIGPYDTLIAGQALARGATLVTHNVREFSRVPGLKVEDWY 130
VapC COG1487
Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];
5-136 3.19e-27

Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];


Pssm-ID: 441096  Cd Length: 128  Bit Score: 98.01  E-value: 3.19e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   5 YMLDTNICSFIMRERPPVVLSRLQSCvnAQHRIVVSAITYAEMRFGAvGRKASPKHAELVTAFVARLDgVLSWDTAAVDA 84
Cdd:COG1487    1 YLLDTNVLIDLLRGRPPAVLAWLAEL--EAGELAISAITLAELLYGA-RRSPDAKRRAALEAFLEAFP-VLPFDAEAARR 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 504709958  85 TAAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDW 136
Cdd:COG1487   77 AGELRAALRRAGRPIGLADLLIAATALAHGLTLVTRNTKDFERIPGLKVEPW 128
PRK13725 PRK13725
tRNA(fMet)-specific endonuclease VapC;
5-137 1.01e-26

tRNA(fMet)-specific endonuclease VapC;


Pssm-ID: 184277  Cd Length: 132  Bit Score: 97.02  E-value: 1.01e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   5 YMLDTNICSFIMRERPPVVLSRLQscVNaQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLDgVLSWDTAAVDA 84
Cdd:PRK13725   4 FMLDTNICIFTIKNKPEHVRERFN--LN-TGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLE-VLDYDTAAATH 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 504709958  85 TAAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDWA 137
Cdd:PRK13725  80 TGQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVPGIRIEDWS 132
PIN_RfVapC2-like cd18734
VapC-like PIN domain of Rickettsia felis VapC2 and related proteins; This subfamily includes ...
 6-136 4.36e-25

VapC-like PIN domain of Rickettsia felis VapC2 and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Rickettsia felis VapC2, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. Rickettsia felis VapC2 cleaves single-stranded RNA. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350301  Cd Length: 129  Bit Score: 92.56  E-value: 4.36e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   6 MLDTNICSFIMRERPPVVLSRLQScVNAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLDgVLSWDTAAVDAT 85
Cdd:cd18734    1 MLDTNICVYLIKKKPDSYYARLEK-LQKNNDIAISSIVLAELQYGVSKSQYKEQNQAALNMFLMKLE-VLPFEEKATFYY 78

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 504709958  86 AAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDW 136
Cdd:cd18734   79 GEIRAELEKSGQVIGGNDLLIAAHALAENATLVTNNIKEFARVPNLILENW 129
PIN_VapC4-5_FitB-like cd18740
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 6-135 4.40e-22

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350307  Cd Length: 126  Bit Score: 84.53  E-value: 4.40e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   6 MLDTNICSFIMRERPPVVLSRLqSCVNAqHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLDgVLSWDTAAVDAT 85
Cdd:cd18740    1 MLDTNTVSHIIKGRPPAVRERL-RAVPM-ASICISSITEAELLYGLAKRPEAKRLHAAVREFLLRVD-VLPWDSSVARTY 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 504709958  86 AAIRADLAARGTPIGANDASIAGHAIAAGAVLVTnNNREFSRVAGLSLED 135
Cdd:cd18740   78 GDLRAALEAQGKTLGPLDMMIAAHALALGATLVT-NDRAFRRVPGLAVED 126
PIN_VapC4-5_FitB-like cd18744
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 6-136 6.64e-20

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350311  Cd Length: 128  Bit Score: 79.07  E-value: 6.64e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   6 MLDTNICSFIMRERPPVVlSRLQSCVNAQHRIVVSAITYAEMRFGAVGRKASpKHAELVTAFVARLDgVLSWDTAAVDAT 85
Cdd:cd18744    1 LLDTDILSALLKGNPKVV-EKFREYLKEYGKITISIITYYEILRGLLAKNAT-KQLEVFEEFCSNNE-VLPLDDESADIA 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 504709958  86 AAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDW 136
Cdd:cd18744   78 AEIYADLRKKGTLIDDADILIAATAIANNLVLVTNNTKHFQRIEGLKLENW 128
PIN_CcVapC1-like cd18736
VapC-like PIN domain of Caulobacter Crescentus VapC1-like and related proteins; This subfamily ...
 6-136 2.98e-17

VapC-like PIN domain of Caulobacter Crescentus VapC1-like and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Caulobacter Crescentus VapC1, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. This subfamily belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. FitB is a toxin of the FitAB TA system. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350303  Cd Length: 123  Bit Score: 72.21  E-value: 2.98e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   6 MLDTNICSFIMRERPPVVLSRLQSCvnAQHRIVVSAITYAEMRFGAvgRKASPKHAELVTAFVARLDgVLSWDTAAVDAT 85
Cdd:cd18736    1 LLDTNICIYLLKGQPPALARRVAAC--EPGEVVISAITLAELAYGV--RRSAPPRRAALDALLEVIP-VLPFDEAAAAAY 75

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 504709958  86 AAIRADLAAR----GTPIGANDASIAghaiaagAVLVTNNNREFSRVAGLSLEDW 136
Cdd:cd18736   76 ARIRAAFPFRrasfDRLIAAHALALG-------LTLVTNNEADFADIPGLRVENW 123
PIN_VapC4-5_FitB-like cd18750
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 6-135 4.56e-17

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350317  Cd Length: 127  Bit Score: 71.89  E-value: 4.56e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   6 MLDTNICSFIMRERPPVvLSRLQScVNAQHrIVVSAITYAEMRFG-AVGRKASPKHAELVTAFVARLDgVLSWDTAAVDA 84
Cdd:cd18750    1 LLDTCTISDFVRGNPNT-LERLKS-TSPSL-IAISSITVMEIEYGlRLNPQRAKKLRPILEAFLSSIT-ILPFDEEDAIA 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 504709958  85 TAAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLED 135
Cdd:cd18750   77 AAAIRAALKKQGTPIGAYDVLIAGTALAHGLILVTSNTKEFQRVDGLVIEN 127
PIN_VapC4-5_FitB-like cd18742
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 7-136 1.46e-15

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350309  Cd Length: 127  Bit Score: 67.95  E-value: 1.46e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   7 LDTNICSFIMRERPPVVlSRLQScvNAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLDgVLSWDTAAVDATA 86
Cdd:cd18742    2 LDTDICIYWLKGNESVK-EKFLE--VGNEPLSISVITYAELYYGAYNSQRVEKNLARVERFKELLP-VLPLSEAIAETFG 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 504709958  87 AIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDW 136
Cdd:cd18742   78 KIKADLRKKGNPIDDFDLLIASTALANNLILVTNNTKHFKRIPGLKIENW 127
PIN_VapC4-5_FitB-like cd18752
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 6-136 7.66e-14

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350319  Cd Length: 130  Bit Score: 63.81  E-value: 7.66e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   6 MLDTNICSFIMRERP-PVVLSRLQSC-VNAQHrivVSAITYAEMRFGAVgRKASPKHA--ELVTAFVARLDgVLSWDTAA 81
Cdd:cd18752    1 LFDTDAISELLKKRPsPPLLERLNSIpREDQF---TSAITVGELYYGAY-RSDRRLRLleELLEPVISRVT-ILPFDRES 75

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 504709958  82 VDATAAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDW 136
Cdd:cd18752   76 ARVYGRLRAELEKKGTPLGEADLQIAAIALAHNLTLVTGNIRHFERIPGLRVENW 130
PIN_VapC4-5_FitB-like cd18743
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 7-136 3.83e-12

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350310  Cd Length: 127  Bit Score: 59.07  E-value: 3.83e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   7 LDTNICSFIMRErppvvlsrlQSCVNAQ------HRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLDgVLSwDTA 80
Cdd:cd18743    2 LDTNICIFLLRG---------KYGVAEKieevglENCFISEITIAELLYGAENSARPEKNRKLVDDFLSLFT-VLP-ISD 70

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 504709958  81 AVDATAAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDW 136
Cdd:cd18743   71 ALEEYAKEKARLRRQGKLIDDFDLLIGATAIANNLTLVTENVKHFARIPGIKLENW 126
PIN_VapC4-5_FitB-like cd18749
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 7-133 4.45e-11

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350316  Cd Length: 126  Bit Score: 56.48  E-value: 4.45e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   7 LDTNICSFIMRERPPV--VLSRLQScvnaqHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLdGVLSWDTAAVDA 84
Cdd:cd18749    2 LDTDVASYLFKGRLLAalYAPRLVG-----KILVISFVTVAELYFWAEKRGWGEKRLQQLEAWLSRY-VVLPYDEEIART 75

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 504709958  85 TAAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSL 133
Cdd:cd18749   76 WGRLRAEAQRRGRPRPTNDAWIAATALTHGLPLATHNVKDFEDFPGLQL 124
PIN_VapC4-5_FitB-like cd18751
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 6-136 8.61e-11

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350318  Cd Length: 133  Bit Score: 55.82  E-value: 8.61e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   6 MLDTNICSFIMRERPPVVLSRLQSCVNAQHRIVVSAITYAEMRFGAvgrkASPKHAELVTAFVARLDG--VLSWDTAAVD 83
Cdd:cd18751    1 LLDTDILSEILKGKNPQVVARARAYLAAFGRYTISVITVMEVVRGL----QKVQRAERLQQFLEMIESaeVLPLDRTAAE 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 504709958  84 ATAAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAG----LSLEDW 136
Cdd:cd18751   77 LAGRIYADLERTGQPIGRADPMIAAIALRHGLVLVTGNTAHYQRIQElgypLVLENW 133
PIN_VapC4-5_FitB-like cd18747
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 7-136 1.80e-10

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350314  Cd Length: 132  Bit Score: 54.85  E-value: 1.80e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   7 LDTNICSFIMRERP-PVVLSRLQSCvnaQHRIVVSAITYAEMRFG----AVGRKASpKHAELVTAFVARLDgVLSWDTAA 81
Cdd:cd18747    2 LDTNILSEPLKPEPnANVLARLERH---EGEIAIAAVVWHELLYGcyrlPEGKRKR-ILEYYLEDVVSGLP-ILPYDEAA 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 504709958  82 VDATAAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLEDW 136
Cdd:cd18747   77 AEWHAEERARLTAIGKTPPFADGQIAAIAVANNLILVTRNTKDFQGFEGLRLENW 131
PIN_RfVapC1-like cd18733
VapC-like PIN domain of Rickettsia felis VapC1 and related proteins; This subfamily includes ...
 6-135 1.81e-09

VapC-like PIN domain of Rickettsia felis VapC1 and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Rickettsia felis VapC1, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350300  Cd Length: 131  Bit Score: 52.22  E-value: 1.81e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   6 MLDTNIcsFIMRERPPVVLSRL-QSCVNAQhrIVVSAITYAEMRFGaVGRKASPKHAELVTAFVARLDG---VLSWDTAA 81
Cdd:cd18733    3 IIDTSV--FIAAERGRLDLEALqQERPDED--VYISAITASELLHG-VHRAKTPERRARREAFVEAILEafpVLDFDLEV 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 504709958  82 VDATAAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLED 135
Cdd:cd18733   78 ARTHARLWAELAKRGITIGAHDLLIAATALAHGYSVLTANVRDFKRIPGLEVEE 131
PIN_VapC4-5_FitB-like cd18753
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 6-103 1.71e-06

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350320  Cd Length: 123  Bit Score: 44.00  E-value: 1.71e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   6 MLDTNICSFIMRERPPVVlsrlqSCVNAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLD-GVLSWDTAAVDA 84
Cdd:cd18753    1 LLDTNAYSALLRGDPAVL-----EALARADEIYLPVIVLGELLFGFRKGSREEENRERLEEFLSSPRvEVLPIDEETAEI 75

                         90
                 ....*....|....*....
gi 504709958  85 TAAIRADLAARGTPIGAND 103
Cdd:cd18753   76 YAEIKSALKKKGTPIPEND 94
PIN_NgFitB-like cd18731
VapC-like PIN domain of Neisseria gonorrhoeae FitB and related proteins; This subfamily ...
 7-104 2.05e-06

VapC-like PIN domain of Neisseria gonorrhoeae FitB and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Neisseria gonorrhoeae FitB toxin of the FitAB toxin/antitoxin (TA) system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. In N. gonorrhoeae, FitA and FitB form a heterodimer: FitA is the DNA binding subunit and FitB contains a ribonuclease activity that is blocked by the presence of FitA. A tetramer of FitAB heterodimers binds DNA from the fitAB upstream promoter region with high affinity. This results in both sequestration of FitAB and repression of fitAB transcription. It is thought that FitAB release from the DNA and subsequent dissociation both slows N. gonorrhoeae replication and transcytosis by an as yet undefined mechanism. The toxin M. tuberculosis VapC is a structural homolog of N. gonorrhoeae FitB, but their antitoxin partners, VapB and FitA, respectively, differ structurally. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350298  Cd Length: 136  Bit Score: 43.95  E-value: 2.05e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   7 LDTNICSFIMRERP-PVVLSRLQScvNAQHRIVVSAITYAEMRFGA----VGRKASPKHAELVTAFVARLDG-VLSWDTA 80
Cdd:cd18731    3 LDTNVVSELMRPAPdPAVLAWLDA--QPAEDLYLTAITVAELLYGIallpDGRRRDALEAAAEALLAEDFAGrILPFDAA 80

                         90       100
                 ....*....|....*....|....
gi 504709958  81 AVDATAAIRADLAARGTPIGANDA 104
Cdd:cd18731   81 AAEAYAEIVAARRRAGRPISVADA 104
PIN pfam01850
PIN domain;
5-131 2.27e-04

PIN domain;


Pssm-ID: 426475  Cd Length: 121  Bit Score: 38.54  E-value: 2.27e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958    5 YMLDTNICSFIMRERPPVVLSRLQscVNAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVARLDGVLSWDTAAVDA 84
Cdd:pfam01850   1 IVLDTSVLIALLRGEPLHEAAREL--LEAAGELVTSAIVLAELLYGLNRRLGLGKAAELVELLLLLSALEVLPIDAELAE 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 504709958   85 TAairADLAARGTPIGANDASIAGHAIAAGAVLVTNNNReFSRVAGL 131
Cdd:pfam01850  79 EA---AELRLKYGKLGPNDALIAATAKEHGAKLITFDED-FARVAKL 121
PIN_VapC4-5_FitB-like cd18738
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 7-135 2.07e-03

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350305  Cd Length: 118  Bit Score: 35.58  E-value: 2.07e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504709958   7 LDTNICSFIMRERPPVVLSRLqscVNAQHRIVVSAITYAE-MRFgavgRKASPKHAELVTAFVARLDgVLSWDTAAVDAT 85
Cdd:cd18738    2 LDTNIIIYYLKGELPESLEEL---LEELNEPAISVITKIElLGW----PKLTPEEEEEIEDFLSLAE-VIPLDDEIARKT 73

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 504709958  86 AAIRadlaaRGTPIGANDASIAGHAIAAGAVLVTNNNREFSRVAGLSLED 135
Cdd:cd18738   74 IELR-----KQYKIKLPDAIIAATALVHGLTLVTRNVKDFKKIPGLKLLN 118
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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