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Conserved domains on  [gi|505199266|ref|WP_015386368|]
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MULTISPECIES: GPW/gp25 family protein [Enterobacteriaceae]

Protein Classification

GPW/gp25 family protein( domain architecture ID 10010043)

GPW/gp25 family protein similar to type VI secretion system (T6SS) baseplate subunit TssE, which is a component of the T6SS baseplate that serves not only as the tail assembly platform, but also docks the tail to the membrane complex

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
W PHA02516
baseplate wedge subunit; Provisional
 7-108 2.78e-50

baseplate wedge subunit; Provisional


:

Pssm-ID: 134016  Cd Length: 103  Bit Score: 154.86  E-value: 2.78e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505199266   7 MSQGNGRTITDTDHLRQSVRDILLTPQGSRIARREYGSLLSELIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITIDS 86
Cdd:PHA02516   1 MNRETGRALSDLEHIRQSIGDILLTPLGSRVMRREYGSLLPDLIDQPQNPALRLQIYAACAMALMRWEPRITLTRVQIER 80

                         90       100
                 ....*....|....*....|...
gi 505199266  87 SFDGSMVVELTGQR-DNGAPVSL 108
Cdd:PHA02516  81 AADGRMTVDITGWHvDTGEPVSL 103
 
Name Accession Description Interval E-value
W PHA02516
baseplate wedge subunit; Provisional
 7-108 2.78e-50

baseplate wedge subunit; Provisional


Pssm-ID: 134016  Cd Length: 103  Bit Score: 154.86  E-value: 2.78e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505199266   7 MSQGNGRTITDTDHLRQSVRDILLTPQGSRIARREYGSLLSELIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITIDS 86
Cdd:PHA02516   1 MNRETGRALSDLEHIRQSIGDILLTPLGSRVMRREYGSLLPDLIDQPQNPALRLQIYAACAMALMRWEPRITLTRVQIER 80

                         90       100
                 ....*....|....*....|...
gi 505199266  87 SFDGSMVVELTGQR-DNGAPVSL 108
Cdd:PHA02516  81 AADGRMTVDITGWHvDTGEPVSL 103
COG3628 COG3628
Phage baseplate assembly protein W [Mobilome: prophages, transposons];
4-111 1.21e-37

Phage baseplate assembly protein W [Mobilome: prophages, transposons];


Pssm-ID: 442846  Cd Length: 119  Bit Score: 123.44  E-value: 1.21e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505199266   4 YIGMSQGNGRT--ITDTDHLRQSVRDILLTPQGSRIARREYGSLLSELIDQPQNPALRLQVMSAVYVALSRWEPRLTLDS 81
Cdd:COG3628    3 EPGFDRATGRVamVSGEEHIRQSIRIILTTPPGERVMRPDFGSGLHDLLFEPNNPATALLIRAAVAEALLRWEPRIEVES 82

                         90       100       110
                 ....*....|....*....|....*....|...
gi 505199266  82 ITIDSSFD--GSMVVELTGQ-RDNGAPVSLSVS 111
Cdd:COG3628   83 VDVTPDPEedGKLLITIDYTvRATNQRFNLVYP 115
GPW_gp25 pfam04965
Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like ...
 19-99 7.12e-17

Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like proteins, found in several systems, including contractile tail bacteriophages, the type VI secretion system (T6SS) and R-type pyocins, which constitute a multiprotein tubular apparatus to attach to and penetrate host cell membranes. Gp25 is a component of the conserved wedge in the inner part of the baseplate and serves as a nucleus for sheath polymerization, playing a critical role in sheath assembly and contraction. The EPR motif (Glu-Pro-Arg, residues 85-87 of gp25) is conserved across all members of the family including orthologs from the RpoS-mediated general stress response system (called IraD). This motif interacts with the 'core bundle' composed of orthologs of T4 gp6 and gp7 proteins in contractile injection systems.


Pssm-ID: 428225  Cd Length: 93  Bit Score: 69.53  E-value: 7.12e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505199266   19 DHLRQSVRDILLTPQGSRIARREYGSLLSELIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITI--DSSFDGSMVVEL 96
Cdd:pfam04965   5 ESVRRDLEILLNTRPGERVSRPNYGCPDLALLFAPNDDATRALIERAIREAILRFEPRIRVVSVEVdpDPDSPGTLRFTI 84


                  ...
gi 505199266   97 TGQ 99
Cdd:pfam04965  85 EYT 87
 
Name Accession Description Interval E-value
W PHA02516
baseplate wedge subunit; Provisional
 7-108 2.78e-50

baseplate wedge subunit; Provisional


Pssm-ID: 134016  Cd Length: 103  Bit Score: 154.86  E-value: 2.78e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505199266   7 MSQGNGRTITDTDHLRQSVRDILLTPQGSRIARREYGSLLSELIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITIDS 86
Cdd:PHA02516   1 MNRETGRALSDLEHIRQSIGDILLTPLGSRVMRREYGSLLPDLIDQPQNPALRLQIYAACAMALMRWEPRITLTRVQIER 80

                         90       100
                 ....*....|....*....|...
gi 505199266  87 SFDGSMVVELTGQR-DNGAPVSL 108
Cdd:PHA02516  81 AADGRMTVDITGWHvDTGEPVSL 103
COG3628 COG3628
Phage baseplate assembly protein W [Mobilome: prophages, transposons];
4-111 1.21e-37

Phage baseplate assembly protein W [Mobilome: prophages, transposons];


Pssm-ID: 442846  Cd Length: 119  Bit Score: 123.44  E-value: 1.21e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505199266   4 YIGMSQGNGRT--ITDTDHLRQSVRDILLTPQGSRIARREYGSLLSELIDQPQNPALRLQVMSAVYVALSRWEPRLTLDS 81
Cdd:COG3628    3 EPGFDRATGRVamVSGEEHIRQSIRIILTTPPGERVMRPDFGSGLHDLLFEPNNPATALLIRAAVAEALLRWEPRIEVES 82

                         90       100       110
                 ....*....|....*....|....*....|...
gi 505199266  82 ITIDSSFD--GSMVVELTGQ-RDNGAPVSLSVS 111
Cdd:COG3628   83 VDVTPDPEedGKLLITIDYTvRATNQRFNLVYP 115
GPW_gp25 pfam04965
Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like ...
 19-99 7.12e-17

Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like proteins, found in several systems, including contractile tail bacteriophages, the type VI secretion system (T6SS) and R-type pyocins, which constitute a multiprotein tubular apparatus to attach to and penetrate host cell membranes. Gp25 is a component of the conserved wedge in the inner part of the baseplate and serves as a nucleus for sheath polymerization, playing a critical role in sheath assembly and contraction. The EPR motif (Glu-Pro-Arg, residues 85-87 of gp25) is conserved across all members of the family including orthologs from the RpoS-mediated general stress response system (called IraD). This motif interacts with the 'core bundle' composed of orthologs of T4 gp6 and gp7 proteins in contractile injection systems.


Pssm-ID: 428225  Cd Length: 93  Bit Score: 69.53  E-value: 7.12e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505199266   19 DHLRQSVRDILLTPQGSRIARREYGSLLSELIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITI--DSSFDGSMVVEL 96
Cdd:pfam04965   5 ESVRRDLEILLNTRPGERVSRPNYGCPDLALLFAPNDDATRALIERAIREAILRFEPRIRVVSVEVdpDPDSPGTLRFTI 84


                  ...
gi 505199266   97 TGQ 99
Cdd:pfam04965  85 EYT 87
25 PHA00415
baseplate wedge subunit
 16-89 3.04e-05

baseplate wedge subunit


Pssm-ID: 222788  Cd Length: 131  Bit Score: 40.37  E-value: 3.04e-05
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 505199266  16 TDTDHLRQSVRDILLTPQGSRIARREYGSLLSELIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITIDSSFD 89
Cdd:PHA00415  28 KGARAIKNSLLGIVTTRKGERPFDPNFGCDISDQLFENMDPLTADTIERNIESAIRNYEPRVYNLNVEVIPVYD 101
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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