|
Name |
Accession |
Description |
Interval |
E-value |
| PRK03103 |
PRK03103 |
DNA polymerase IV; Reviewed |
1-395 |
0e+00 |
|
DNA polymerase IV; Reviewed
Pssm-ID: 235104 [Multi-domain] Cd Length: 409 Bit Score: 704.07 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 1 MERVVALVDMNSFYASCHQAFNPELDGKEVIVAGEPERRTGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPL 80
Cdd:PRK03103 1 MERVILLVDMQSFYASVEKAANPELKGRPVIVSGDPERRSGVVLAACPLAKAYGVKTAERLWEAQQKCPDLVVVKPRMQR 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 81 YIDFSSRILHIMRDFTDLVEPFSIDEAFIDFSGIINLWGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGL--- 157
Cdd:PRK03103 81 YIDVSLQITRILEDFTDLVEPFSIDEQFLDVTGSQKLFGSPLEIAQKIQQRIMRETGVYARVGIGPNKLLAKMACDNfak 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 158 QKPNGLTIIKTvNDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGKNGEMLWYCAQGLDHSPV 237
Cdd:PRK03103 161 KNPDGLFTLDK-EDVPADLWPLPVRKLFGVGSRMEKHLRRMGIRTIGQLANTPLERLKKRWGINGEVLWRTANGIDYSPV 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 238 TSGSPDTDKSIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRDTQLEF---ISRSMTIPDPTNLA 314
Cdd:PRK03103 240 TPHSLDRQKAIGHQMTLPRDYRGFEEIKVVLLELCEEVCRRARAKGYMGRTVSVSLRGADFDWptgFSRQMTLPEPTNLA 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 315 GEIYQAACKLLHKNWDqSWAVRLVGVTLSNLKTDDYIQYDLFGEKEKQVKLAKVYDKICNRFGERAIFRGISLTEVSLYA 394
Cdd:PRK03103 320 MEVYEAACKLFHRHWD-GKPVRRVGVTLSNLVSDDVWQLSLFGDRERKRSLGYVMDDIKNRFGPTAILRASSLTEAGQAF 398
|
.
gi 506238461 395 R 395
Cdd:PRK03103 399 E 399
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
3-343 |
1.35e-146 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 418.78 E-value: 1.35e-146
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 3 RVVALVDMNSFYASCHQAFNPELDGKEVIVAGEPERrtGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPLYI 82
Cdd:COG0389 1 RRILHVDMDAFYASVEQRDRPELRGKPVAVGGDNNR--GVVAAASYEARAFGVRSGMPLFQARRLCPDLVVLPPDFELYR 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 83 DFSSRILHIMRDFTDLVEPFSIDEAFIDFSGIINLWGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQKPNG 162
Cdd:COG0389 79 DVSRRVMAILERYTPLVEPLSIDEAFLDVTGSARLFGSAEAIARRIRRRIRRETGLTVSVGIAPNKFLAKIASDLAKPDG 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 163 LTIIkTVNDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGKNGEMLWYCAQGLDHSPVTSGSP 242
Cdd:COG0389 159 LTVI-PPGEVAAFLAPLPVEKLWGVGPKTAEKLARLGIRTIGDLAALPRAELRRRFGKVGERLYRLARGIDPRPVEPRRP 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 243 dtDKSIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRDTQLEFISRSMTIPDPTNLAGEIYQAAC 322
Cdd:COG0389 238 --RKSIGVERTFGEDLTDLEELEAALRRLAERLAERLRRQGLGARTVTVKLRTSDFRTTTRSRTLPEPTDDTAELLRAAR 315
|
330 340
....*....|....*....|.
gi 506238461 323 KLLHKNWDQSWAVRLVGVTLS 343
Cdd:COG0389 316 ELLERIYRPGRPVRLLGVRLS 336
|
|
| PolY_Pol_IV_kappa |
cd03586 |
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ... |
8-345 |
2.06e-135 |
|
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.
Pssm-ID: 176459 [Multi-domain] Cd Length: 334 Bit Score: 390.34 E-value: 2.06e-135
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 8 VDMNSFYASCHQAFNPELDGKEVIVAGEPERrtGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPLYIDFSSR 87
Cdd:cd03586 3 IDMDAFYASVEQRDNPELKGKPVAVGGSSDR--GVVSTASYEARKFGVRSAMPIFQAKKLCPNLIFVPPRFDKYREVSRQ 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 88 ILHIMRDFTDLVEPFSIDEAFIDFSGIINLWGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQKPNGLTIIk 167
Cdd:cd03586 81 IMEILREYTPLVEPLSIDEAYLDVTDYVRLFGSATEIAKEIRARIREETGLTASAGIAPNKFLAKIASDLNKPNGLTVI- 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 168 TVNDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGKNGEMLWYCAQGLDHSPVTsgsPDTD-K 246
Cdd:cd03586 160 PPEDVEEFLAPLPVRKIPGVGKVTAEKLKELGIKTIGDLAKLDVELLKKLFGKSGRRLYELARGIDNRPVE---PDRErK 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 247 SIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRDTQLEFISRSMTIPDPTNLAGEIYQAACKLLH 326
Cdd:cd03586 237 SIGVERTFSEDLTDPEELLEELLELAEELAERLRKRGLKGRTVTVKLKYADFSTRTRSRTLPEPTDDAEDIYELALELLE 316
|
330
....*....|....*....
gi 506238461 327 KNWDQSwAVRLVGVTLSNL 345
Cdd:cd03586 317 ELLDGR-PIRLLGVRLSGL 334
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
8-155 |
5.45e-62 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 196.26 E-value: 5.45e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 8 VDMNSFYASCHQAFNPELDGKEVIVAGEPERrtGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPLYIDFSSR 87
Cdd:pfam00817 1 IDMDAFFASVELLRDPELKGKPVAVGGGNGR--GIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVVPPDLELYRRASRK 78
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 506238461 88 ILHIMRDF-TDLVEPFSIDEAFIDFSGIINLWGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAA 155
Cdd:pfam00817 79 IFEILRRFsTPKVEQASIDEAFLDLTGLEKLFGAEEALAKRLRREIAEETGLTCSIGIAPNKLLAKLAS 147
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| PRK03103 |
PRK03103 |
DNA polymerase IV; Reviewed |
1-395 |
0e+00 |
|
DNA polymerase IV; Reviewed
Pssm-ID: 235104 [Multi-domain] Cd Length: 409 Bit Score: 704.07 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 1 MERVVALVDMNSFYASCHQAFNPELDGKEVIVAGEPERRTGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPL 80
Cdd:PRK03103 1 MERVILLVDMQSFYASVEKAANPELKGRPVIVSGDPERRSGVVLAACPLAKAYGVKTAERLWEAQQKCPDLVVVKPRMQR 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 81 YIDFSSRILHIMRDFTDLVEPFSIDEAFIDFSGIINLWGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGL--- 157
Cdd:PRK03103 81 YIDVSLQITRILEDFTDLVEPFSIDEQFLDVTGSQKLFGSPLEIAQKIQQRIMRETGVYARVGIGPNKLLAKMACDNfak 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 158 QKPNGLTIIKTvNDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGKNGEMLWYCAQGLDHSPV 237
Cdd:PRK03103 161 KNPDGLFTLDK-EDVPADLWPLPVRKLFGVGSRMEKHLRRMGIRTIGQLANTPLERLKKRWGINGEVLWRTANGIDYSPV 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 238 TSGSPDTDKSIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRDTQLEF---ISRSMTIPDPTNLA 314
Cdd:PRK03103 240 TPHSLDRQKAIGHQMTLPRDYRGFEEIKVVLLELCEEVCRRARAKGYMGRTVSVSLRGADFDWptgFSRQMTLPEPTNLA 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 315 GEIYQAACKLLHKNWDqSWAVRLVGVTLSNLKTDDYIQYDLFGEKEKQVKLAKVYDKICNRFGERAIFRGISLTEVSLYA 394
Cdd:PRK03103 320 MEVYEAACKLFHRHWD-GKPVRRVGVTLSNLVSDDVWQLSLFGDRERKRSLGYVMDDIKNRFGPTAILRASSLTEAGQAF 398
|
.
gi 506238461 395 R 395
Cdd:PRK03103 399 E 399
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
3-343 |
1.35e-146 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 418.78 E-value: 1.35e-146
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 3 RVVALVDMNSFYASCHQAFNPELDGKEVIVAGEPERrtGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPLYI 82
Cdd:COG0389 1 RRILHVDMDAFYASVEQRDRPELRGKPVAVGGDNNR--GVVAAASYEARAFGVRSGMPLFQARRLCPDLVVLPPDFELYR 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 83 DFSSRILHIMRDFTDLVEPFSIDEAFIDFSGIINLWGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQKPNG 162
Cdd:COG0389 79 DVSRRVMAILERYTPLVEPLSIDEAFLDVTGSARLFGSAEAIARRIRRRIRRETGLTVSVGIAPNKFLAKIASDLAKPDG 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 163 LTIIkTVNDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGKNGEMLWYCAQGLDHSPVTSGSP 242
Cdd:COG0389 159 LTVI-PPGEVAAFLAPLPVEKLWGVGPKTAEKLARLGIRTIGDLAALPRAELRRRFGKVGERLYRLARGIDPRPVEPRRP 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 243 dtDKSIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRDTQLEFISRSMTIPDPTNLAGEIYQAAC 322
Cdd:COG0389 238 --RKSIGVERTFGEDLTDLEELEAALRRLAERLAERLRRQGLGARTVTVKLRTSDFRTTTRSRTLPEPTDDTAELLRAAR 315
|
330 340
....*....|....*....|.
gi 506238461 323 KLLHKNWDQSWAVRLVGVTLS 343
Cdd:COG0389 316 ELLERIYRPGRPVRLLGVRLS 336
|
|
| PolY_Pol_IV_kappa |
cd03586 |
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ... |
8-345 |
2.06e-135 |
|
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.
Pssm-ID: 176459 [Multi-domain] Cd Length: 334 Bit Score: 390.34 E-value: 2.06e-135
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 8 VDMNSFYASCHQAFNPELDGKEVIVAGEPERrtGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPLYIDFSSR 87
Cdd:cd03586 3 IDMDAFYASVEQRDNPELKGKPVAVGGSSDR--GVVSTASYEARKFGVRSAMPIFQAKKLCPNLIFVPPRFDKYREVSRQ 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 88 ILHIMRDFTDLVEPFSIDEAFIDFSGIINLWGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQKPNGLTIIk 167
Cdd:cd03586 81 IMEILREYTPLVEPLSIDEAYLDVTDYVRLFGSATEIAKEIRARIREETGLTASAGIAPNKFLAKIASDLNKPNGLTVI- 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 168 TVNDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGKNGEMLWYCAQGLDHSPVTsgsPDTD-K 246
Cdd:cd03586 160 PPEDVEEFLAPLPVRKIPGVGKVTAEKLKELGIKTIGDLAKLDVELLKKLFGKSGRRLYELARGIDNRPVE---PDRErK 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 247 SIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRDTQLEFISRSMTIPDPTNLAGEIYQAACKLLH 326
Cdd:cd03586 237 SIGVERTFSEDLTDPEELLEELLELAEELAERLRKRGLKGRTVTVKLKYADFSTRTRSRTLPEPTDDAEDIYELALELLE 316
|
330
....*....|....*....
gi 506238461 327 KNWDQSwAVRLVGVTLSNL 345
Cdd:cd03586 317 ELLDGR-PIRLLGVRLSGL 334
|
|
| PRK01810 |
PRK01810 |
DNA polymerase IV; Validated |
3-389 |
6.25e-120 |
|
DNA polymerase IV; Validated
Pssm-ID: 179337 [Multi-domain] Cd Length: 407 Bit Score: 353.95 E-value: 6.25e-120
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 3 RVVALVDMNSFYASCHQAFNPELDGKEVIVAGEPERRTGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPLYI 82
Cdd:PRK01810 5 RVIFHVDMNSFFASVEIAYDPSLQGKPLAVAGNEKERKGIIVTCSYEARAYGIRTTMPLWEAKRLCPQLIVRRPNFDRYR 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 83 DFSSRILHIMRDFTDLVEPFSIDEAFIDFSGIINLwGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQKPNG 162
Cdd:PRK01810 85 EASRQMFQILSEFTPLVQPVSIDEGYLDITDCYAL-GSPLEIAKMIQQRLLTELQLPCSIGIAPNKFLAKMASDMKKPLG 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 163 LTIIKTvNDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGKNGEMLWYCAQGLDHSPVTSGSP 242
Cdd:PRK01810 164 ITVLRK-RDVPEMLWPLPVGEMHGIGEKTAEKLKDIGIQTIGDLAKADEHILRAKLGINGVRLQRRANGIDDRPVDPEAI 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 243 DTDKSIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRDTQLEFISRSMTIPDPTNLAGEIYQAAC 322
Cdd:PRK01810 243 YQFKSVGNSTTLSHDMDEEKELLDVLRRLSKSVSKRLQKKTVVSYNVQIMIRYHDRRTITRSKTLKNPIWEKRDIFQAAS 322
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 506238461 323 KLLHKNWDQSwAVRLVGVTLSNL--KTDDYIQYDLFGEKE--KQVKLAKVYDKICNRFGERAIFRGISLTE 389
Cdd:PRK01810 323 RLFKQHWNGD-PVRLLGVTATDLewKTEAVKQLDLFSFEEdaKEEPLLAVIDQINDKYGMPLLQRGSQLLR 392
|
|
| PolY_Pol_V_umuC |
cd01700 |
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion ... |
6-344 |
9.99e-103 |
|
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.
Pssm-ID: 176454 [Multi-domain] Cd Length: 344 Bit Score: 307.55 E-value: 9.99e-103
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 6 ALVDMNSFYASCHQAFNPELDGKEVIVAGEPERRtgiVLAASYQAKAKGVGTGMALWEAKKLCPD--GYFFKPNYPLYID 83
Cdd:cd01700 1 ALVDCNSFYASCERVFRPLLLGRPLVVLSNNDGC---VIARSPEAKALGIKMGSPYFKVPDLLERhgVAVFSSNYALYGD 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 84 FSSRILHIMRDFTDLVEPFSIDEAFIDFSGIINlWGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGL----QK 159
Cdd:cd01700 78 MSRRIMSILERFSPDVEVYSIDESFLDLTGSLR-FGDLEELARKIRRRILQETGIPVTVGIGPTKTLAKLANDLakkkNP 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 160 PNGLTIIKTVNDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGKNGEMLWYCAQGLDHSPVTS 239
Cdd:cd01700 157 YGGVVDLTDEEVRDKLLKILPVGDVWGIGRRTAKKLNAMGIHTAGDLAQADPDLLRKKFGVVGERLVRELNGIDCLPLEE 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 240 GSPDTdKSIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLR----DTQLEFISRSMTIPDPTNLAG 315
Cdd:cd01700 237 YPPPK-KSIGSSRSFGRDVTDLDELKQALAEYAERAAEKLRRQKSVARTISVFIGtsgfSRQPKYYSATNTLPYPTNDTR 315
|
330 340
....*....|....*....|....*....
gi 506238461 316 EIYQAACKLLHKNWDQSWAVRLVGVTLSN 344
Cdd:cd01700 316 EIVKAALRLLYAIYRPGYAYRKAGVMLSD 344
|
|
| PRK02794 |
PRK02794 |
DNA polymerase IV; Provisional |
5-388 |
4.88e-89 |
|
DNA polymerase IV; Provisional
Pssm-ID: 179473 [Multi-domain] Cd Length: 419 Bit Score: 275.27 E-value: 4.88e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 5 VALVDMNSFYASCHQAFNPELDGKEVIVAGEpeRRtGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPLYIDF 84
Cdd:PRK02794 38 IAHIDCDAFYASVEKRDNPELRDKPVIIGGG--KR-GVVSTACYIARIHGVRSAMPMFKALKLCPDAVVIKPDMEKYVRV 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 85 SSRILHIMRDFTDLVEPFSIDEAFIDFSGIINLWG-PPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQKPNGL 163
Cdd:PRK02794 115 GREVRAMMQALTPLVEPLSIDEAFLDLSGTERLHGaPPAVVLARFARRVEREIGITVSVGLSYNKFLAKIASDLDKPRGF 194
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 164 TIIKTVnDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGKNGEMLWYCAQGLDHSPVtsgSPD 243
Cdd:PRK02794 195 SVIGRA-EALAFLAPKPVGIIWGVGPATAARLARDGIRTIGDLQRADEADLMRRFGSMGLRLWRLARGIDDRKV---SPD 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 244 TD-KSIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRDTQLEFISRSMTIPDPTNLAGEIYQAAC 322
Cdd:PRK02794 271 REaKSVSAETTFETDLSDFEDLEPILWRLSEKVSRRLKAAGLAGRTVTLKLKTADFRLRTRRRTLEDPTQLADRIFRTAR 350
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 506238461 323 KLLHKNWDQSwAVRLVGVTLSNLK-TDDYIQYDLFGEKEKQVKLA-KVYDKICNRFGERAIFRGISLT 388
Cdd:PRK02794 351 ELLEKETDGT-AFRLIGIGVSDLSpADEADPPDLLDPQATRRAAAeRAIDALRAKFGAAAVETGRALR 417
|
|
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
10-356 |
1.81e-88 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 271.22 E-value: 1.81e-88
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 10 MNSFYASCHQAFNPELDGKEVIVAGEPERRtGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPLYIDFSSRIL 89
Cdd:PRK02406 1 MDCFYAAVEMRDNPELRGKPVAVGGSPGRR-GVISTCNYEARKFGVRSAMPTAQALKLCPDLIFVPGRFDVYKEVSRQIR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 90 HIMRDFTDLVEPFSIDEAFIDFSGIINLWGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQKPNGLTIIKtv 169
Cdd:PRK02406 80 EIFRRYTDLIEPLSLDEAYLDVTDNKLCIGSATLIAQEIRQDIFEELGLTASAGVAPNKFLAKIASDWNKPNGLFVIT-- 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 170 ndYRNV--FYSK-PVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGKNGEMLWYCAQGLDHSPVtsgSPDTD- 245
Cdd:PRK02406 158 --PEEVdaFLATlPVEKIPGVGKVTAEKLHALGIYTCADLQKYDLAELIRHFGKFGRRLYERARGIDERPV---KPDREr 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 246 KSIGQQRTLPRDIRGFKDIQIIILELCEMVARRAR--QGGYVGRTVFLTLRDTQLEFISRSMTiPDPTNLAGEIYQAACK 323
Cdd:PRK02406 233 KSVGVERTFAEDLYDLEACLAELPRLAEKLERRLEraKPDKRIKTVGVKLKFADFQQTTKEHT-ADPLDKADLIELLAQA 311
|
330 340 350
....*....|....*....|....*....|...
gi 506238461 324 LLHKNWDQswAVRLVGVTLSNLKTDDYIQYDLF 356
Cdd:PRK02406 312 LLRRLGGR--GVRLLGVGVTLLEPQLERQLLLD 342
|
|
| PRK03858 |
PRK03858 |
DNA polymerase IV; Validated |
9-387 |
1.95e-86 |
|
DNA polymerase IV; Validated
Pssm-ID: 179663 [Multi-domain] Cd Length: 396 Bit Score: 267.62 E-value: 1.95e-86
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 9 DMNSFYASCHQAFNPELDGKEVIVAGeperrtGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPLYIDFSSRI 88
Cdd:PRK03858 10 DLDSFYASVEQRDDPALRGRPVIVGG------GVVLAASYEAKAYGVRTAMGGRQARRLCPQAVVVPPRMSAYSRASKAV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 89 LHIMRDFTDLVEPFSIDEAFIDFSGIINLWGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQKPNGLTIIKT 168
Cdd:PRK03858 84 FEVFRDTTPLVEGLSIDEAFLDVGGLRRISGTPVQIAARLRRRVREEVGLPITVGVARTKFLAKVASQVAKPDGLLVVPP 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 169 vNDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGK-NGEMLWYCAQGLDHSPVTSGSpdTDKS 247
Cdd:PRK03858 164 -DRELAFLHPLPVRRLWGVGPVTAAKLRAHGITTVGDVAELPESALVSLLGPaAGRHLHALAHNRDPRRVETGR--RRRS 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 248 IGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRDTQLEFISRSMTIPDPTNLAGEIYQAACKLLhk 327
Cdd:PRK03858 241 VGAQRALGRGPNSPAEVDAVVVALVDRVARRMRAAGRTGRTVVLRLRFDDFTRATRSHTLPRPTASTATLLAAARDLV-- 318
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 506238461 328 nwDQSWA------VRLVGVTLSNLKTDDYIQYDL-FGEKEKQVKLAKVYDKICNRFGERAIFRGISL 387
Cdd:PRK03858 319 --AAAAPliaergLTLVGFAVSNLDDDGAQQLELpFGLRRPGSALDAALDAVRDRFGNAAVTRAVLL 383
|
|
| PolY |
cd00424 |
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases ... |
8-343 |
3.02e-79 |
|
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Most TLS polymerases are members of the Y-family, including Pol eta, Pol kappa/IV, Pol iota, Rev1, and Pol V, which is found exclusively in bacteria. In eukaryotes, the B-family polymerase Pol zeta also functions as a TLS polymerase. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176453 [Multi-domain] Cd Length: 343 Bit Score: 247.66 E-value: 3.02e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 8 VDMNSFYASCHQAFNPELDGKEVIVAGEPERRTGIVlAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPLYIDFSSR 87
Cdd:cd00424 3 IDFDNFFASVEQLARPELKGRPVVVVPFNSDSTCVI-ACSYEARKYGVKRGMPVREARKMCPNLILVPARLDLYRRLSER 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 88 ILHIMRDFTDLVEPFSIDEAFIDFSGIINLWGPPVEIAAKIRQRILSEIG-VQCSIGIGPNKLIAKMAAGLQKPNGLTII 166
Cdd:cd00424 82 LLSELEEVAPLVEVASIDELFLDLTGSARLLGLGSEVALRIKRHIAEQLGgITASIGIASNKLLAKLAAKYAKPDGLTIL 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 167 KtvNDYRNVFYSK-PVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWG-KNGEMLWYCAQGLDHSPVTsgSPDT 244
Cdd:cd00424 162 D--PEDLPGFLSKlPLTDLPGIGAVTAKRLEAVGINPIGDLLAASPDALLALWGgVSGERLWYALRGIDDEPLS--PPRP 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 245 DKSIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRD------TQLEFISRSMTIPDPTNlAGEIY 318
Cdd:cd00424 238 RKSFSHERVLPRDSRNAEDARPLLRLLLEKLARRLRRDGRGATRLRLWLRTvdgrwsGHADIPSRSAPRPISTE-DGELL 316
|
330 340
....*....|....*....|....*..
gi 506238461 319 QAACKLLHKNWDQS--WAVRLVGVTLS 343
Cdd:cd00424 317 HALDKLWRALLDDKgpRRLRRLGVRLS 343
|
|
| PRK03348 |
PRK03348 |
DNA polymerase IV; Provisional |
1-363 |
7.76e-76 |
|
DNA polymerase IV; Provisional
Pssm-ID: 235118 [Multi-domain] Cd Length: 454 Bit Score: 242.15 E-value: 7.76e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 1 MERVVALVDMNSFYASCHQAFNPELDGKEVIVAGEPERrtGIVLAASYQAKAKGVGTGMALWEAKKLCP-DGYFFKPNYP 79
Cdd:PRK03348 3 AQRWVLHLDMDAFFASVEQLTRPTLRGRPVLVGGLGGR--GVVAGASYEARVFGARSAMPMHQARRLVGnGAVVLPPRFV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 80 LYIDFSSRILHIMRDFTDLVEPFSIDEAFIDFSGIINLWGPPVE-IAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQ 158
Cdd:PRK03348 81 VYRAASRRVFDTLRELSPVVEQLSFDEAFVEPAELAGASAEEVEaFAERLRARVREETGLPASVGAGSGKQIAKIASGLA 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 159 KPNGLTIIKtVNDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGK-NGEMLWYCAQGLDHSPV 237
Cdd:PRK03348 161 KPDGIRVVP-PGEERELLAPLPVRRLWGIGPVTEEKLHRLGIETIGDLAALSEAEVANLLGAtVGPALHRLARGIDDRPV 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 238 TsgsPDTD-KSIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRDTQLEFISRSMTIPDPTNLAGE 316
Cdd:PRK03348 240 A---ERAEaKQISAESTFAVDLTTRAQLREAIERIAEHAHRRLLKDGRGARTVTVKLRKSDFSTLTRSATLPYATDDAAV 316
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 506238461 317 IYQAACKLLhKNWDQSWAVRLVGVTLSNLKtdDYIQYDLFGEKEKQV 363
Cdd:PRK03348 317 LAATARRLL-LDPDEIGPIRLVGVGFSGLS--DVRQESLFPELDLAR 360
|
|
| PRK14133 |
PRK14133 |
DNA polymerase IV; Provisional |
1-356 |
2.74e-74 |
|
DNA polymerase IV; Provisional
Pssm-ID: 184529 [Multi-domain] Cd Length: 347 Bit Score: 235.00 E-value: 2.74e-74
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 1 MERVVALVDMNSFYASCHQAFNPELDGKEVIVAGEPERrtGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPL 80
Cdd:PRK14133 1 MDRVIIHVDMDAFFASVEQMDNPKLKGKPVIVGGISER--GVVSTCSYEARKYGVHSAMPVFMAKKRCPHGIFLPVRHER 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 81 YIDFSSRILHIMRDFTDLVEPFSIDEAFIDFSGIINlwgPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQKP 160
Cdd:PRK14133 79 YKEVSKNIFKILYEVTPIVEPVSIDEAYLDITNIKE---EPIKIAKYIKKKVKKETGLTLSVGISYNKFLAKLASDWNKP 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 161 NGLTIIkTVNDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGKNGEMLWYCAQGLDHSPVTSG 240
Cdd:PRK14133 156 DGIKII-TEDMIPDILKPLPISKVHGIGKKSVEKLNNIGIYTIEDLLKLSREFLIEYFGKFGVEIYERIRGIDYREVEVS 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 241 SpdTDKSIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRDTQLEFISRSMTIPDPTNLAGEIYQA 320
Cdd:PRK14133 235 R--ERKSIGKETTLKKDTKDKEELKKYLKDFSNIISEELKKRNLYGKTVTVKIKTSDFQTHTKSKTLNDYIRDKEEIYNV 312
|
330 340 350
....*....|....*....|....*....|....*.
gi 506238461 321 ACKLLhKNWDQSWAVRLVGVTLSNLKTDDYIQYDLF 356
Cdd:PRK14133 313 ACEIL-EHINIKEPIRLIGLSVSNLSENKIEQLSFL 347
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
8-155 |
5.45e-62 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 196.26 E-value: 5.45e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 8 VDMNSFYASCHQAFNPELDGKEVIVAGEPERrtGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPLYIDFSSR 87
Cdd:pfam00817 1 IDMDAFFASVELLRDPELKGKPVAVGGGNGR--GIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVVPPDLELYRRASRK 78
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 506238461 88 ILHIMRDF-TDLVEPFSIDEAFIDFSGIINLWGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAA 155
Cdd:pfam00817 79 IFEILRRFsTPKVEQASIDEAFLDLTGLEKLFGAEEALAKRLRREIAEETGLTCSIGIAPNKLLAKLAS 147
|
|
| PRK03352 |
PRK03352 |
DNA polymerase IV; Validated |
1-342 |
1.07e-58 |
|
DNA polymerase IV; Validated
Pssm-ID: 179564 [Multi-domain] Cd Length: 346 Bit Score: 194.47 E-value: 1.07e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 1 MERVVALVDMNSFYASCHQAFNPELDGKEVIV--AGEPERRTGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNY 78
Cdd:PRK03352 3 MPRWVLHVDLDQFIAAVELLRRPELAGLPVIVggNGDPTEPRKVVTCASYEARAFGVRAGMPLRTAARRCPDAVFLPSDP 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 79 PLYIDFSSRILHIMRDFTDLVEPFSIDEAFIDFSGiinlwGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQ 158
Cdd:PRK03352 83 AAYDAASEEVMATLRDLGVPVEVWGWDEAFLGVDT-----DDPEALAEEIRAAVLERTGLSCSVGIGDNKLRAKIATGFA 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 159 KPNGLTIIKTVNdYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWG-KNGEMLWYCAQGLDHSPV 237
Cdd:PRK03352 158 KPAGVFRLTDAN-WMAVMGDRPTDALWGVGPKTAKRLAALGITTVADLAAADPAELAATFGpTTGPWLLLLARGGGDTEV 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 238 TSGsPDTDKSIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRDTQLEFISRSMTIPDPTNLAGEI 317
Cdd:PRK03352 237 SAE-PWVPRSRSREVTFPQDLTDRAEVESAVRELARRVLDEVVAEGRPVTRVAVKVRTATFYTRTKIRKLPEPTTDPDVI 315
|
330 340
....*....|....*....|....*
gi 506238461 318 YQAACKLLHKnWDQSWAVRLVGVTL 342
Cdd:PRK03352 316 EAAALDVLDR-FELDRPVRLLGVRL 339
|
|
| PolY_Rev1 |
cd01701 |
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. ... |
2-345 |
6.58e-49 |
|
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Rev1 has both structural and enzymatic roles. Structurally, it is believed to interact with other nonclassical polymerases and replication machinery to act as a scaffold. Enzymatically, it catalyzes the specific insertion of dCMP opposite abasic sites. Rev1 interacts with the Rev7 subunit of the B-family TLS polymerase Pol zeta (Rev3/Rev7). Rev1 is known to actively promote the introduction of mutations, potentially making it a significant target for cancer treatment.
Pssm-ID: 176455 [Multi-domain] Cd Length: 404 Bit Score: 170.57 E-value: 6.58e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 2 ERVVALVDMNSFYASCHQAFNPELDGKEVIVAGEPERRTGIVlAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPLY 81
Cdd:cd01701 46 QRIIMHVDFDCFFVSVSIRNRPDLKGKPVAVCHGKGPNSEIA-SCNYEARSYGIKNGMWVGQAKKLCPQLVTLPYDFEAY 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 82 IDFSSRILHIMRDFTDLVEPFSIDEAFIDFSGII-NLWGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQKP 160
Cdd:cd01701 125 EEVSLTFYEILASYTDNIEAVSCDEALIDITSLLeETYELPEELAEAIRNEIRETTGCSASVGIGPNILLARLATRKAKP 204
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 161 NGLTIIK--TVNDyrnVFYSKPVRKLFGIGSRYEKHLRKFNVFTIG--ELANYPVEILKKRWG-KNGEMLW-YCaQGLDH 234
Cdd:cd01701 205 DGQYHLSaeKVEE---FLSQLKVGDLPGVGSSLAEKLVKLFGDTCGglELRSKTKEKLQKVLGpKTGEKLYdYC-RGIDD 280
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 235 SPVTsgSPDTDKSIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTL--RDT-------------QLE 299
Cdd:cd01701 281 RPVT--GEKERKSVSAEINYGIRFTNVDDVEQFLQRLSEELSKRLEESNVTGRQITLKLmkRAPgapieppkymghgICD 358
|
330 340 350 360
....*....|....*....|....*....|....*....|....*.
gi 506238461 300 FISRSMTIPDPTNLAGEIYQAACKLLHKNWDQSWAVRLVGVTLSNL 345
Cdd:cd01701 359 SFSKSSTLGVATDDSGVIGTEAKKLFRDLSIPPEELRGVGIQVTKL 404
|
|
| PolY_Pol_eta |
cd01702 |
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ... |
6-344 |
7.61e-47 |
|
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.
Pssm-ID: 176456 [Multi-domain] Cd Length: 359 Bit Score: 163.64 E-value: 7.61e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 6 ALVDMNSFYASCHQAFNPELDGKEVIVAgeperRTGIVLAASYQAKAKGVGTGMALWEAKKLCPD--------------- 70
Cdd:cd01702 1 AHIDMDAFFAQVEQVRLGLLRNDPVAVV-----QWNSIIAVSYAARAFGVTRFMTIDEAKKKCPDlilahvatykkgede 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 71 -GYFFKP-------NYPLYIDFSSRILHIMRDFTDLVEPFSIDEAFIDFSGiinlwgppvEIAAKIRQRILSEIGVQCSI 142
Cdd:cd01702 76 aDYHENPsparhkvSLDPYRRASRKILNILKRFGDVVEKASIDEAYLDLGS---------RIVEEIRQQVYDELGYTCSA 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 143 GIGPNKLIAKMAAGLQKPNGLTIIKT--VNDYrnvFYSKPVRKLFGIGSRYEKHLR-KFNVFTIGELANY---PVEILKK 216
Cdd:cd01702 147 GIAHNKMLAKLASGMNKPNAQTILRNdaVASF---LSSLPITSIRGLGGKLGEEIIdLLGLPTEGDVAGFrssESDLQEH 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 217 RWGKNGEMLWYCAQGLDHSPVTSGSpdTDKSIGQQRTLPR-DIRGFKDIQIIILELCEMVARRAR----QGGYVGRTVFL 291
Cdd:cd01702 224 FGEKLGEWLYNLLRGIDHEPVKPRP--LPKSMGSSKNFPGkTALSTEDVQHWLLVLASELNSRLEddryENNRRPKTLVL 301
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 292 TLR-DTQLEFISRSMTIPDPTnlAGEIYQAACKLLHKNW------DQSWAVRLVGVTLSN 344
Cdd:cd01702 302 SLRqRGDGVRRSRSCALPRYD--AQKIVKDAFKLIKAINeeglglAWNYPLTLLSLSFTK 359
|
|
| PRK01216 |
PRK01216 |
DNA polymerase IV; Validated |
4-345 |
7.34e-45 |
|
DNA polymerase IV; Validated
Pssm-ID: 179251 [Multi-domain] Cd Length: 351 Bit Score: 158.41 E-value: 7.34e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 4 VVALVDMNSFYASCHQAFNPELDGKEVIV---AGEPERrTGIVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPL 80
Cdd:PRK01216 2 IILFVDFDYFFAQVEEVLNPSLKGKPVVVcvySGRFED-SGAVATANYEARKLGIKAGMPIVEAKKILPNAVYLPMRKEV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 81 YIDFSSRILHIMRDFTDLVEPFSIDEAFIDFSGIINLWGPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQKP 160
Cdd:PRK01216 81 YQQVSNRIMKLLREYSEKIEIASIDEAYLDISDKVKNYQDAYNLGLEIKNKILEKEKITVTVGISKNKVFAKIAADMAKP 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 161 NGLTIIKTvNDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGK-NGEMLWYCAQGLDHSPVts 239
Cdd:PRK01216 161 NGIKVIDD-EEVKRFINELDIADIPGIGDITAEKLKKLGVNKLVDTLRIEFDELKGIIGEaKAKYLFSLARNEYNEPV-- 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 240 gSPDTDKSIGQQRTLPRDIRgfkDIQIIILELcemvaRRARQGGY-------VGRTVFLTLRDtqLEFISRSMTIPDPTN 312
Cdd:PRK01216 238 -RARVRKSIGRYVTLPRNTR---DLEEIKPYL-----KRAIEEAYykldgipKAIHVVAIMED--LDIVSRGRTFTHGIS 306
|
330 340 350
....*....|....*....|....*....|....
gi 506238461 313 LAgEIYQAACKLLHKNWDQ-SWAVRLVGVTLSNL 345
Cdd:PRK01216 307 KE-TAYREAVRLLQKILEEdERKIRRIGVRFSKI 339
|
|
| IMS_C |
pfam11799 |
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss). |
246-349 |
2.71e-32 |
|
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss).
Pssm-ID: 463354 [Multi-domain] Cd Length: 104 Bit Score: 117.28 E-value: 2.71e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 246 KSIGQQRTLPRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRDTQLEFISRSMTIPDPTNLAGEIYQAACKLL 325
Cdd:pfam11799 2 KSIGAERTFGRDLTDLEELREALLELAEELAERLRRQGLVARTVTVKIRYSDFRTITRSVTLPSPTDDTDEIYRAALRLL 81
|
90 100
....*....|....*....|....
gi 506238461 326 HKNWDQsWAVRLVGVTLSNLKTDD 349
Cdd:pfam11799 82 RRLYRG-RPVRLLGVSLSNLVPEG 104
|
|
| PolY_Pol_iota |
cd01703 |
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ... |
7-344 |
4.74e-32 |
|
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.
Pssm-ID: 176457 [Multi-domain] Cd Length: 379 Bit Score: 124.50 E-value: 4.74e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 7 LVDMNSFYASCHQAFNPELDGKEVIVagepeRRTGIVLAASYQAKAKGVGTGMALWEAKKLCPD-----GYFFKPnyplY 81
Cdd:cd01703 2 HLDLDCFYAQVEEIRDPSLKSKPLGI-----QQKYIVVTCNYEARRLGVKKLMSIKDAKEICPDlvlvnGEDLTP----F 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 82 IDFSSRILHIMRDFT--DLVEPFSIDEAFIDFSGIINLWGPpvEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQK 159
Cdd:cd01703 73 RDMSKKVYRLLRSYSwnDRVERLGFDENFMDVTEMRLLVAS--HIAYEMRERIENELGLTCCAGIASNKLLAKLVGSVNK 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 160 PNGLT-IIKTVNDYRNVFY-SKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPV---------------EILKKRWGK-N 221
Cdd:cd01703 151 PNQQTtLLPPSCADLMDFMdLHDLRKIPGIGYKTAAKLEAHGISSVRDLQEFSNrnrqtvgaapsllelLLMVKEFGEgI 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 222 GEMLWYCAQGLDHSPVTSgSPDTDKSIGQQRTLPR-DIRGFKDIQIIILELCEMVARRARQG--------GYVGRTVFLT 292
Cdd:cd01703 231 GQRIWKLLFGRDTSPVKP-ASDFPQQISIEDSYKKcSLEEIREARNKIEELLASLLERMKQDlqevkagdGRRPHTLRLT 309
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 293 LR---DTQLEF--ISRSMTIP---------DPTNLAGEIYQAACKLLHK----NWDQSWAVRLVGVTLSN 344
Cdd:cd01703 310 LRrytSTKKHYnrESKQAPIPshvfqkltgGNEIAARPIEKILMRLFRElvppKNVKGFNLTLLNVCFTN 379
|
|
| umuC |
PRK03609 |
translesion error-prone DNA polymerase V subunit UmuC; |
6-386 |
5.17e-31 |
|
translesion error-prone DNA polymerase V subunit UmuC;
Pssm-ID: 179607 [Multi-domain] Cd Length: 422 Bit Score: 122.57 E-value: 5.17e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 6 ALVDMNSFYASCHQAFNPELDGKEVIVAGEPErrtGIVLAASYQAKAKGVGTGMALWEAKKL-CPDG-YFFKPNYPLYID 83
Cdd:PRK03609 3 ALCDVNSFYASCETVFRPDLRGKPVVVLSNND---GCVIARSAEAKALGIKMGDPWFKQKDLfRRCGvVCFSSNYELYAD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 84 FSSRILHIMRDFTDLVEPFSIDEAFIDFSGIINLWGpPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQKP--- 160
Cdd:PRK03609 80 MSNRVMSTLEELSPRVEIYSIDEAFCDLTGVRNCRD-LTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKwqr 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 161 --NGLTIIKTVNDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGK---------NGEMlwyCA 229
Cdd:PRK03609 159 qtGGVVDLSNLERQRKLLSLQPVEEVWGVGRRISKKLNAMGIKTALDLADTNIRFIRKHFNVvlertvrelRGEP---CL 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 230 qGLDHSPVTSGSPDTDKSIGQQRTLPRDIRgfkdiQIIilelCEMVARRA---RQGGYVGRTVFLTLRDTQLEFI----- 301
Cdd:PRK03609 236 -SLEEFAPTKQEIVCSRSFGERITDYESMR-----QAI----CSYAARAAeklRGEHQYCRFISTFVKTSPFALNepyyg 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 302 -SRSMTIPDPTNLAGEIYQAACKLLHKNWDQSWAVRLVGVTLSNLKTDDYIQYDLFGE---KEKQVKLAKVYDKICNRFG 377
Cdd:PRK03609 306 nSASVKLLTPTQDSRDIIAAATRALDAIWRDGHRYQKAGVMLGDFFSQGVAQLNLFDDnapRPGSEQLMKVLDTLNAKSG 385
|
410
....*....|..
gi 506238461 378 ERAIF---RGIS 386
Cdd:PRK03609 386 KGTLYfagQGIA 397
|
|
| PolY_like |
cd03468 |
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that ... |
30-259 |
4.05e-18 |
|
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176458 [Multi-domain] Cd Length: 335 Bit Score: 84.36 E-value: 4.05e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 30 VIVAGEPERRtgiVLAASYQAKAKGVGTGMALWEAKKLCPDGYFFkPNYPLY-IDFSSRILHIMRDFTDLVEPFSIDEAF 108
Cdd:cd03468 26 AVVERKKAGR---ILACNAAARAAGVRPGMPLAEALALCPNLQVV-EYDPEAdARALQELALWLLRFTPLVALDGPDGLL 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 109 IDFSGIINLWGPPVEIAAKIRQRiLSEIGVQCSIGIGPNKLIAKMAAglQKPNGLTIIKTVNDYRNVFYSK--PVRKLfG 186
Cdd:cd03468 102 LDVTGCLHLFGGEDALAASLRAA-LATLGLSARAGIADTPGAAWLLA--RAGGGRGVLRREALAAALVLLAplPVAAL-R 177
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 506238461 187 IGSRYEKHLRKFNVFTIGELANYPVEILKKRWGKNGEMLWYCAQGLDHSPVTSGSPDTDKSIGQQRTLPRDIR 259
Cdd:cd03468 178 LPPETVELLARLGLRTLGDLAALPRAELARRFGLALLLRLDQAYGRDPEPLLFSPPPPAFDFRLELQLEEPIA 250
|
|
| PTZ00205 |
PTZ00205 |
DNA polymerase kappa; Provisional |
8-345 |
2.52e-17 |
|
DNA polymerase kappa; Provisional
Pssm-ID: 140232 [Multi-domain] Cd Length: 571 Bit Score: 83.53 E-value: 2.52e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 8 VDMNSFYASCHQAFNPELDGKEVIVAGEPERRTgivlaASYQAKAKGVGTGMALWEAKKLCPDGYFFKPNYPLYIDFSSR 87
Cdd:PTZ00205 138 LDMDMFYAAVEIKKHPEYAAIPLAIGTMTMLQT-----ANYVARGRGIRQGMPGFLALKICPNLLILPPDFDAYNEESNT 212
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 88 ILHIMRDFTDLVEPFSIDEAFIDFSGIINLW---GPPVEIAAKIRQRILSEIGVQCSIGIGPNKLIAKMAAGLQKPNGLT 164
Cdd:PTZ00205 213 VRRIVAEYDPNYISFGLDELTLEVSAYIERFegtKTAEDVASELRVRVFGETKLTASAGIGPTAALAKIASNINKPNGQH 292
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 165 IIK--TVNDYRNVFYSKPVRKLFGIGSRYEKHLRKFNVFTIGELANYPVEILKKRWGKNGEMLWYCAQGLDHSPVTSGSP 242
Cdd:PTZ00205 293 DLNlhTRGDVMTYVRDLGLRSVPGVGKVTEALLKGLGITTLSDIYNRRVELCYILHNNLFRFLLGASIGIMQWPDAATAA 372
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506238461 243 DTD----------KSIGQQRTLpRDIRGFKDIQIIILELCEMVARRARQGGYVGRTVFLTLRDTQLEFISRSMTIPDPTN 312
Cdd:PTZ00205 373 NTEncegatggqrKAISSERSF-TTPRTKEGLQEMVDTVFNGAYEEMRKSELMCRQISLTIRWASYRYQQYTKSLIQYSD 451
|
330 340 350
....*....|....*....|....*....|...
gi 506238461 313 LAGEIYQAACKLLHKNWDQSWAVRLVGVTLSNL 345
Cdd:PTZ00205 452 DSATLRRAVDGLLLPHAAKYSEMCLLGVRFLDL 484
|
|
| |
