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Conserved domains on  [gi|506990777|ref|WP_016081371|]
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MULTISPECIES: GNAT family N-acetyltransferase [Bacillus]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10136841)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.1.-
Gene Ontology:  GO:0008080
PubMed:  10940244|9175471|15581578
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 65-140 7.05e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


:

Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 47.27  E-value: 7.05e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 506990777  65 FLLAIDtsNDKIIGTIETGPAstlitsctgGVLKNLYEIGTVFILPEYQRKGIGSLLLHTMFLTLLSRGITEYCLD 140
Cdd:cd04301    1 FLVAED--DGEIVGFASLSPD---------GSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
 
Name Accession Description Interval E-value
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 65-140 7.05e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 47.27  E-value: 7.05e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 506990777  65 FLLAIDtsNDKIIGTIETGPAstlitsctgGVLKNLYEIGTVFILPEYQRKGIGSLLLHTMFLTLLSRGITEYCLD 140
Cdd:cd04301    1 FLVAED--DGEIVGFASLSPD---------GSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 102-178 2.80e-07

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 46.57  E-value: 2.80e-07
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 506990777 102 EIGTVFILPEYQRKGIGSLLLHTMFLTLLSRGITEYCLDSGYKNAQSI-WMKKFG-KPSYVLKDYWGEssDHMIWKKSL 178
Cdd:COG0456   15 EIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIaLYEKLGfEEVGERPNYYGD--DALVMEKEL 91
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 37-155 1.42e-06

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 45.20  E-value: 1.42e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506990777   37 LLDDIENEINSKKQYLKSDFESNGESRYFLLAIDtsnDKIIGTIETGPastlitsctGGVLKNLYEIGTVFILPEYQRKG 116
Cdd:pfam00583   8 LSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEED---GELVGFASLSI---------IDDEPPVGEIEGLAVAPEYRGKG 75

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 506990777  117 IGSLLLHTMFLTLLSRGITEYCLDSGYKNAQSI-WMKKFG 155
Cdd:pfam00583  76 IGTALLQALLEWARERGCERIFLEVAADNLAAIaLYEKLG 115
 
Name Accession Description Interval E-value
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 65-140 7.05e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 47.27  E-value: 7.05e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 506990777  65 FLLAIDtsNDKIIGTIETGPAstlitsctgGVLKNLYEIGTVFILPEYQRKGIGSLLLHTMFLTLLSRGITEYCLD 140
Cdd:cd04301    1 FLVAED--DGEIVGFASLSPD---------GSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 102-178 2.80e-07

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 46.57  E-value: 2.80e-07
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 506990777 102 EIGTVFILPEYQRKGIGSLLLHTMFLTLLSRGITEYCLDSGYKNAQSI-WMKKFG-KPSYVLKDYWGEssDHMIWKKSL 178
Cdd:COG0456   15 EIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIaLYEKLGfEEVGERPNYYGD--DALVMEKEL 91
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 37-155 1.42e-06

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 45.20  E-value: 1.42e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506990777   37 LLDDIENEINSKKQYLKSDFESNGESRYFLLAIDtsnDKIIGTIETGPastlitsctGGVLKNLYEIGTVFILPEYQRKG 116
Cdd:pfam00583   8 LSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEED---GELVGFASLSI---------IDDEPPVGEIEGLAVAPEYRGKG 75

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 506990777  117 IGSLLLHTMFLTLLSRGITEYCLDSGYKNAQSI-WMKKFG 155
Cdd:pfam00583  76 IGTALLQALLEWARERGCERIFLEVAADNLAAIaLYEKLG 115
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 102-155 2.31e-06

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 45.04  E-value: 2.31e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 506990777 102 EIGTVFILPEYQRKGIGSLLLHTMFLTLLSRGITEYCLDSGYKNAQSI-WMKKFG 155
Cdd:COG0454   60 ELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIrFYERLG 114
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
56-178 2.72e-06

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 45.37  E-value: 2.72e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506990777  56 FESNGESRYFLLAIDtSNDKIIGTIETGPASTlitsctGGVLKNLYEIgTVFILPEYQRKGIGSLLLHTMFLTLLSRGIT 135
Cdd:COG1247   44 FAAILAPGRPVLVAE-EDGEVVGFASLGPFRP------RPAYRGTAEE-SIYVDPDARGRGIGRALLEALIERARARGYR 115

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 506990777 136 EYCLDSGYKNAQSIWM-KKFG-KPSYVLKD---YWGESSDHMIWKKSL 178
Cdd:COG1247  116 RLVAVVLADNEASIALyEKLGfEEVGTLPEvgfKFGRWLDLVLMQKRL 163
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 61-155 4.94e-06

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 44.21  E-value: 4.94e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506990777  61 ESRYFLLAIDtsNDKIIGTIetgpasTLITSCTGgvlknLYEIGTVFILPEYQRKGIGSLLLHTMFLTLLSRGITEYCLD 140
Cdd:COG1246   26 EIGEFWVAEE--DGEIVGCA------ALHPLDED-----LAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLL 92

                         90
                 ....*....|....*
gi 506990777 141 SgYKNAQSIWmKKFG 155
Cdd:COG1246   93 T-TSAAIHFY-EKLG 105
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 61-147 7.23e-06

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 42.44  E-value: 7.23e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506990777   61 ESRYFLLAIDtsNDKIIGTIETGPASTLITSCtggvlknlyeIGTVFILPEYQRKGIGSLLLHTMFLTLLSRGITEYCLD 140
Cdd:pfam13508   1 PGGRFFVAED--DGKIVGFAALLPLDDEGALA----------ELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELE 68


                  ....*..
gi 506990777  141 SGYKNAQ 147
Cdd:pfam13508  69 TTNRAAA 75
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
55-141 8.22e-06

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 43.54  E-value: 8.22e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506990777  55 DFESNGESRYFLLAIDtsNDKIIGTIetgpastLITSCTGGVLKNLYEIGTVFILPEYQRKGIGSLLLHTMFLTLLSRGI 134
Cdd:COG3153   31 RLREDPAAGLSLVAED--DGEIVGHV-------ALSPVDIDGEGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERGA 101


                 ....*..
gi 506990777 135 TEYCLDS 141
Cdd:COG3153  102 RAVVLLG 108
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
102-149 1.09e-04

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 39.51  E-value: 1.09e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 506990777 102 EIGTVFILPEYQRKGIGSLLLHTMFLTLLSRGITEYCLDSGYKNAQSI 149
Cdd:COG3393   17 EISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYVDADNPAAR 64
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 25-125 3.76e-04

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 38.79  E-value: 3.76e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 506990777   25 ITNTYKNEGLSQLLDDIENEinSKKQYLksdfeSNGESRYFLLaidTSNDKIIGTIEtgpastlitsctggvLKNLYEIG 104
Cdd:pfam13673   1 EAPDYSEEGIETFYEFISPE--ALRERI-----DQGEYFFFVA---FEGGQIVGVIA---------------LRDRGHIS 55

                          90       100
                  ....*....|....*....|.
gi 506990777  105 TVFILPEYQRKGIGSLLLHTM 125
Cdd:pfam13673  56 LLFVDPDYQGQGIGKALLEAV 76
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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