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Conserved domains on  [gi|507049171|ref|WP_016120189|]
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GNAT family N-acetyltransferase [Bacillus cereus]

Protein Classification

GNAT family protein( domain architecture ID 106742)

GNAT (Gcn5-related N-acetyltransferase) family protein similar to N-acetyltransferases that catalyze the transfer of an acetyl group from acetyl-CoA to a substrate

PubMed:  15581578

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NAT_SF super family cl17182
N-Acyltransferase superfamily: Various enyzmes that characteristicly catalyze the transfer of ...
 18-130 1.37e-14

N-Acyltransferase superfamily: Various enyzmes that characteristicly catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase which catalyze the transfer of an acetyl group to a substrate. The mechanism is an ordered Bi-Bi ternary complex kinetic mechanism for most GNATs: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and then CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/ph enylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


The actual alignment was detected with superfamily member pfam13527:

Pssm-ID: 473072 [Multi-domain]  Cd Length: 124  Bit Score: 68.75  E-value: 1.37e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 507049171   18 EQLFPLFEEVFGITSQTLQDFsekgYWDYTYKA---LSFLQDDNAIANVAAFSLPLLINGEKINATGIQSVMTHPNYRRR 94
Cdd:pfam13527  10 DEVLRLLEYAFQDEDSPELRE----YFRPLLEEgrvLGAFDDGELVSTLALYPFELNVPGKTLPAAGITGVATYPEYRGR 85

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 507049171   95 GLMKQLFSKMIEEIDEQCEC-AVLFTENPELYKPFGF 130
Cdd:pfam13527  86 GVMSRLLRRSLEEMRERGVPlSFLYPSSYPIYRRFGY 122
 
Name Accession Description Interval E-value
Acetyltransf_9 pfam13527
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 18-130 1.37e-14

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 404421 [Multi-domain]  Cd Length: 124  Bit Score: 68.75  E-value: 1.37e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 507049171   18 EQLFPLFEEVFGITSQTLQDFsekgYWDYTYKA---LSFLQDDNAIANVAAFSLPLLINGEKINATGIQSVMTHPNYRRR 94
Cdd:pfam13527  10 DEVLRLLEYAFQDEDSPELRE----YFRPLLEEgrvLGAFDDGELVSTLALYPFELNVPGKTLPAAGITGVATYPEYRGR 85

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 507049171   95 GLMKQLFSKMIEEIDEQCEC-AVLFTENPELYKPFGF 130
Cdd:pfam13527  86 GVMSRLLRRSLEEMRERGVPlSFLYPSSYPIYRRFGY 122
Eis COG4552
Predicted acetyltransferase [General function prediction only];
12-130 8.72e-11

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 61.84  E-value: 8.72e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 507049171  12 LEEPRREQLFPLFEEVFG--ITSQTLQDFSEKGYWDYTYKALsflQDDNAIANVAAFSLPLLINGEKINATGIQSVMTHP 89
Cdd:COG4552    6 LTEDDLDAFARLLAYAFGpePDDEELEAYRPLLEPGRVLGVF---DDGELVGTLALYPFTLNVGGARVPMAGITGVAVAP 82

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 507049171  90 NYRRRGLMKQLFSKMIEEI-DEQCECAVLFTENPELYKPFGF 130
Cdd:COG4552   83 EHRRRGVARALLREALAELrERGQPLSALYPFEPGFYRRFGY 124
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 82-111 5.39e-03

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 34.94  E-value: 5.39e-03
                         10        20        30
                 ....*....|....*....|....*....|
gi 507049171  82 IQSVMTHPNYRRRGLMKQLFSKMIEEIDEQ 111
Cdd:cd04301   28 IGDLAVLPEYRGKGIGSALLEAAEEEARER 57
 
Name Accession Description Interval E-value
Acetyltransf_9 pfam13527
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 18-130 1.37e-14

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 404421 [Multi-domain]  Cd Length: 124  Bit Score: 68.75  E-value: 1.37e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 507049171   18 EQLFPLFEEVFGITSQTLQDFsekgYWDYTYKA---LSFLQDDNAIANVAAFSLPLLINGEKINATGIQSVMTHPNYRRR 94
Cdd:pfam13527  10 DEVLRLLEYAFQDEDSPELRE----YFRPLLEEgrvLGAFDDGELVSTLALYPFELNVPGKTLPAAGITGVATYPEYRGR 85

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 507049171   95 GLMKQLFSKMIEEIDEQCEC-AVLFTENPELYKPFGF 130
Cdd:pfam13527  86 GVMSRLLRRSLEEMRERGVPlSFLYPSSYPIYRRFGY 122
Eis COG4552
Predicted acetyltransferase [General function prediction only];
12-130 8.72e-11

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 61.84  E-value: 8.72e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 507049171  12 LEEPRREQLFPLFEEVFG--ITSQTLQDFSEKGYWDYTYKALsflQDDNAIANVAAFSLPLLINGEKINATGIQSVMTHP 89
Cdd:COG4552    6 LTEDDLDAFARLLAYAFGpePDDEELEAYRPLLEPGRVLGVF---DDGELVGTLALYPFTLNVGGARVPMAGITGVAVAP 82

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 507049171  90 NYRRRGLMKQLFSKMIEEI-DEQCECAVLFTENPELYKPFGF 130
Cdd:COG4552   83 EHRRRGVARALLREALAELrERGQPLSALYPFEPGFYRRFGY 124
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
78-140 3.17e-09

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 52.99  E-value: 3.17e-09
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 507049171  78 NATGIQSVMTHPNYRRRGLMKQLFSKMIEEIDEQ-CECAVLFT--ENPE---LYKPFGFQIVQEYLMTV 140
Cdd:COG3393   14 GVAEISGVYTHPEYRGRGLASALVAALAREALARgARTPFLYVdaDNPAarrLYERLGFRPVGEYATVL 82
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
18-136 2.78e-06

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 46.23  E-value: 2.78e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 507049171  18 EQLFPLFEEVFGIT--SQTLQDFSEKGYWDYTYKALsflqDDNAIANVAAFSlPLLINGEkINATGIQSVMTHPNYRRRG 95
Cdd:COG3153   10 EAIAALLRAAFGPGreAELVDRLREDPAAGLSLVAE----DDGEIVGHVALS-PVDIDGE-GPALLLGPLAVDPEYRGQG 83

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 507049171  96 LMKQLFSKMIEEIDEQ-CECAVLFT--ENPELYKPFGFQIVQEY 136
Cdd:COG3153   84 IGRALMRAALEAARERgARAVVLLGdpSLLPFYERFGFRPAGEL 127
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 18-130 6.91e-06

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 44.43  E-value: 6.91e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 507049171   18 EQLFPLFEEVFGITSQTLQDFSEKGYWDYTYKALSFLQDDNAIANVAAFSlpllINGEKINATGIQSVMTHPNYRRRGLM 97
Cdd:pfam00583   2 EALYELLSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLS----IIDDEPPVGEIEGLAVAPEYRGKGIG 77

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 507049171   98 KQLFSKMIEE-IDEQCECAVLFTENP-----ELYKPFGF 130
Cdd:pfam00583  78 TALLQALLEWaRERGCERIFLEVAADnlaaiALYEKLGF 116
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 82-135 1.35e-04

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 41.13  E-value: 1.35e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 507049171  82 IQSVMTHPNYRRRGLMKQLFSKMIEEIDEQ-CECAVLFT--ENPELYKPFGFQIVQE 135
Cdd:COG1246   55 LRSLAVHPDYRGRGIGRRLLEALLAEARELgLKRLFLLTtsAAIHFYEKLGFEEIDK 111
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 82-135 3.72e-04

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 38.87  E-value: 3.72e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 507049171  82 IQSVMTHPNYRRRGLMKQLFSKMIEEIDEQ-CECAVLFT--ENP---ELYKPFGFQIVQE 135
Cdd:COG0456   16 IEDLAVDPEYRGRGIGRALLEAALERARERgARRLRLEVreDNEaaiALYEKLGFEEVGE 75
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 79-132 7.10e-04

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 37.82  E-value: 7.10e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 507049171   79 ATGIQSVMTHPNYRRRGLMKQLFSKMIEEIDEQ-CECAVLFTENP--ELYKPFGFQI 132
Cdd:pfam13508  28 ALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGgIKLLELETTNRaaAFYEKLGFEE 84
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
80-133 4.08e-03

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 37.28  E-value: 4.08e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 507049171  80 TGIQSVMTHPNYRRRGLMKQLFSKMIEEIDEQ-CECAVL--FTENP---ELYKPFGFQIV 133
Cdd:COG1247   81 TAEESIYVDPDARGRGIGRALLEALIERARARgYRRLVAvvLADNEasiALYEKLGFEEV 140
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 82-111 5.39e-03

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 34.94  E-value: 5.39e-03
                         10        20        30
                 ....*....|....*....|....*....|
gi 507049171  82 IQSVMTHPNYRRRGLMKQLFSKMIEEIDEQ 111
Cdd:cd04301   28 IGDLAVLPEYRGKGIGSALLEAAEEEARER 57
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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