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Conserved domains on  [gi|513828456|ref|WP_016513327|]
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MULTISPECIES: glycoside hydrolase family 99-like domain-containing protein [Bacillus]

Protein Classification

glycoside hydrolase family 99-like domain-containing protein( domain architecture ID 10184107)

glycoside hydrolase family 99-like domain-containing protein is typically found within O-antigen biosynthesis clusters and bears resemblance to the GH71/GH99 family of alpha-1,2-mannosidases

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Glyco_tran_WbsX cd11579
Glycosyl hydrolase family 99-like domain of WbsX-like glycosyltransferases; Members of this ...
1-346 0e+00

Glycosyl hydrolase family 99-like domain of WbsX-like glycosyltransferases; Members of this domain family are found in proteins within O-antigen biosynthesis clusters in Gram negative bacteria, where they may function as glycosyl hydrolases and typically co-occur with glycosyltransferase domains. They bear resemblance to GH71 and the GH99 family of alpha-1,2-mannosidases and may share a similar cataltyic site and mechanism. The O-antigens are essential lipopolysaccharides in gram-negative bacteria's outer membrane and have been linked to pathogenicity.


:

Pssm-ID: 211420 [Multi-domain]  Cd Length: 347  Bit Score: 532.50  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456   1 MKIIAFYLPQFHQIKENDRWWGKGFTEWTNTKSARPLFSGHYQPREPYQNFYYDLTSPSVRKWQAEIAKAYGIYGFCYYH 80
Cdd:cd11579    1 MKVIAFYLPQFHPIPENDEWWGKGFTEWTNVKKAKPLFEGHYQPRVPLDLGYYDLRDPEVREKQAELAKEYGIDGFCYYH 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456  81 YWFKGKRLLETPFNDVLKMKEPDFPFCLSWANEPWTKSWDGLDKHILMPQDYGDISDWKEHFDYLLQAFQDERYIYIDDK 160
Cdd:cd11579   81 YWFGGKRLLEKPLEELLLSGEPDFPFCLCWANENWTRRWDGRDKEELLEQQYYGEEDWKEHFRYLLPYFKDPRYIKIDGK 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456 161 PLFIIYRPGHIPDCEKMLNYWNALARENGLKGIYFAETLNSFPL-PNINGFDASIQFEPFYTIAHDSSSDINKVIYDSDM 239
Cdd:cd11579  161 PVFLIYRPELIPDPKETLELWRELAREAGLPGLYLVAVASFGIEdPRDLGFDAAVEFPPHYVVASELSKSRKLLKLKKGF 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456 240 QLNVWDYDKVWMYILQRSLPKKKTFPGAFVDWDNTARRKNaNSSIFVGSTPEKFTIYLSKQIHRTYSF-YNSEFLFINAW 318
Cdd:cd11579  241 GGNVYDYDDLVKYILNRPDPDYPYYPGVFPGWDNTPRRGK-RGTIFVNSTPELFKKWLRKAIERAKARpEDERLVFINAW 319
                        330       340
                 ....*....|....*....|....*...
gi 513828456 319 NEWAEGTYLEPDKKYGFSYLEGVKNAID 346
Cdd:cd11579  320 NEWAEGAYLEPDLKYGYGYLEALKEALE 347
 
Name Accession Description Interval E-value
Glyco_tran_WbsX cd11579
Glycosyl hydrolase family 99-like domain of WbsX-like glycosyltransferases; Members of this ...
1-346 0e+00

Glycosyl hydrolase family 99-like domain of WbsX-like glycosyltransferases; Members of this domain family are found in proteins within O-antigen biosynthesis clusters in Gram negative bacteria, where they may function as glycosyl hydrolases and typically co-occur with glycosyltransferase domains. They bear resemblance to GH71 and the GH99 family of alpha-1,2-mannosidases and may share a similar cataltyic site and mechanism. The O-antigens are essential lipopolysaccharides in gram-negative bacteria's outer membrane and have been linked to pathogenicity.


Pssm-ID: 211420 [Multi-domain]  Cd Length: 347  Bit Score: 532.50  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456   1 MKIIAFYLPQFHQIKENDRWWGKGFTEWTNTKSARPLFSGHYQPREPYQNFYYDLTSPSVRKWQAEIAKAYGIYGFCYYH 80
Cdd:cd11579    1 MKVIAFYLPQFHPIPENDEWWGKGFTEWTNVKKAKPLFEGHYQPRVPLDLGYYDLRDPEVREKQAELAKEYGIDGFCYYH 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456  81 YWFKGKRLLETPFNDVLKMKEPDFPFCLSWANEPWTKSWDGLDKHILMPQDYGDISDWKEHFDYLLQAFQDERYIYIDDK 160
Cdd:cd11579   81 YWFGGKRLLEKPLEELLLSGEPDFPFCLCWANENWTRRWDGRDKEELLEQQYYGEEDWKEHFRYLLPYFKDPRYIKIDGK 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456 161 PLFIIYRPGHIPDCEKMLNYWNALARENGLKGIYFAETLNSFPL-PNINGFDASIQFEPFYTIAHDSSSDINKVIYDSDM 239
Cdd:cd11579  161 PVFLIYRPELIPDPKETLELWRELAREAGLPGLYLVAVASFGIEdPRDLGFDAAVEFPPHYVVASELSKSRKLLKLKKGF 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456 240 QLNVWDYDKVWMYILQRSLPKKKTFPGAFVDWDNTARRKNaNSSIFVGSTPEKFTIYLSKQIHRTYSF-YNSEFLFINAW 318
Cdd:cd11579  241 GGNVYDYDDLVKYILNRPDPDYPYYPGVFPGWDNTPRRGK-RGTIFVNSTPELFKKWLRKAIERAKARpEDERLVFINAW 319
                        330       340
                 ....*....|....*....|....*...
gi 513828456 319 NEWAEGTYLEPDKKYGFSYLEGVKNAID 346
Cdd:cd11579  320 NEWAEGAYLEPDLKYGYGYLEALKEALE 347
Glyco_tran_WbsX pfam14307
Glycosyltransferase WbsX; Members of this family are found in within O-antigen biosynthesis ...
3-339 0e+00

Glycosyltransferase WbsX; Members of this family are found in within O-antigen biosynthesis clusters in Gram negative bacteria, where they are predicted to function as glycosyltransferases.


Pssm-ID: 464132 [Multi-domain]  Cd Length: 311  Bit Score: 522.45  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456    3 IIAFYLPQFHQIKENDRWWGKGFTEWTNTKSARPLFSGHYQPREPYQNFYYDLTSPSVRKWQAEIAKAYGIYGFCYYHYW 82
Cdd:pfam14307   1 VIAFYLPQFHPIPENDEWWGKGFTEWTNVKKAKPLFKGHYQPRLPADLGYYDLRLPEVRRAQAELAKEYGIYGFCYYHYW 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456   83 FKGKRLLETPFNDVLKMKEPDFPFCLSWANEPWTKSWDGLDKHILMPQDYGDiSDWKEHFDYLLQAFQDERYIYIDDKPL 162
Cdd:pfam14307  81 FGGKRLLEKPLEELLESKEPDFPFCLCWANESWTRRWDGSDKEILIEQDYGE-EDDKAHFDYLLPYFKDPRYIKVDGKPL 159
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456  163 FIIYRPGHIPDCEKMLNYWNALARENGLKGIYFAETLNSFPL-PNINGFDASIQFepfytiahdsssdinkviydsdmql 241
Cdd:pfam14307 160 FLIYRPDLIPDPKETIERWRELAKENGLGGIYLVGVQSFGDEdPRDLGFDAAVEF------------------------- 214
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456  242 nvwDYDKVWMYILQRSLPKKKTFPGAFVDWDNTARRKNaNSSIFVGSTPEKFTIYLSKQIHRTYSFYNSE-FLFINAWNE 320
Cdd:pfam14307 215 ---DYDKLVRRILNRPPPKYKLFPGVFPGWDNTPRRGK-RGTIFHGATPEKFEKWLEKAVERAKQKPDEErIVFINAWNE 290
                         330
                  ....*....|....*....
gi 513828456  321 WAEGTYLEPDKKYGFSYLE 339
Cdd:pfam14307 291 WAEGAYLEPDLKFGYAYLE 309
RgpF COG3754
Lipopolysaccharide biosynthesis protein [Cell wall/membrane/envelope biogenesis];
1-106 5.10e-17

Lipopolysaccharide biosynthesis protein [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442968 [Multi-domain]  Cd Length: 152  Bit Score: 77.02  E-value: 5.10e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456   1 MKIIAFYLPQFHQI----------------------KENDRWWGKgfTEWTNtksaRPLFSGHYQ-----------PREP 47
Cdd:COG3754    4 VALFAFYLPQADELlltndsifgpiaplsplfdrmePENDDWWGL--GEWTN----KPLFPGHLQsyflvfrkralASPA 77
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 513828456  48 YQNFYYDLTSPS-----VRKWQAEIAKAYGIYGFCyYHYWFKGKRL----LETPFNDVLkmKEPDFPF 106
Cdd:COG3754   78 FRDFWYDLRLIEdkddvIRRYEAGLARALGIAGFC-YHYWFDGKRLrtrlLERPWDELL--ENGGFPF 142
 
Name Accession Description Interval E-value
Glyco_tran_WbsX cd11579
Glycosyl hydrolase family 99-like domain of WbsX-like glycosyltransferases; Members of this ...
1-346 0e+00

Glycosyl hydrolase family 99-like domain of WbsX-like glycosyltransferases; Members of this domain family are found in proteins within O-antigen biosynthesis clusters in Gram negative bacteria, where they may function as glycosyl hydrolases and typically co-occur with glycosyltransferase domains. They bear resemblance to GH71 and the GH99 family of alpha-1,2-mannosidases and may share a similar cataltyic site and mechanism. The O-antigens are essential lipopolysaccharides in gram-negative bacteria's outer membrane and have been linked to pathogenicity.


Pssm-ID: 211420 [Multi-domain]  Cd Length: 347  Bit Score: 532.50  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456   1 MKIIAFYLPQFHQIKENDRWWGKGFTEWTNTKSARPLFSGHYQPREPYQNFYYDLTSPSVRKWQAEIAKAYGIYGFCYYH 80
Cdd:cd11579    1 MKVIAFYLPQFHPIPENDEWWGKGFTEWTNVKKAKPLFEGHYQPRVPLDLGYYDLRDPEVREKQAELAKEYGIDGFCYYH 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456  81 YWFKGKRLLETPFNDVLKMKEPDFPFCLSWANEPWTKSWDGLDKHILMPQDYGDISDWKEHFDYLLQAFQDERYIYIDDK 160
Cdd:cd11579   81 YWFGGKRLLEKPLEELLLSGEPDFPFCLCWANENWTRRWDGRDKEELLEQQYYGEEDWKEHFRYLLPYFKDPRYIKIDGK 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456 161 PLFIIYRPGHIPDCEKMLNYWNALARENGLKGIYFAETLNSFPL-PNINGFDASIQFEPFYTIAHDSSSDINKVIYDSDM 239
Cdd:cd11579  161 PVFLIYRPELIPDPKETLELWRELAREAGLPGLYLVAVASFGIEdPRDLGFDAAVEFPPHYVVASELSKSRKLLKLKKGF 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456 240 QLNVWDYDKVWMYILQRSLPKKKTFPGAFVDWDNTARRKNaNSSIFVGSTPEKFTIYLSKQIHRTYSF-YNSEFLFINAW 318
Cdd:cd11579  241 GGNVYDYDDLVKYILNRPDPDYPYYPGVFPGWDNTPRRGK-RGTIFVNSTPELFKKWLRKAIERAKARpEDERLVFINAW 319
                        330       340
                 ....*....|....*....|....*...
gi 513828456 319 NEWAEGTYLEPDKKYGFSYLEGVKNAID 346
Cdd:cd11579  320 NEWAEGAYLEPDLKYGYGYLEALKEALE 347
Glyco_tran_WbsX pfam14307
Glycosyltransferase WbsX; Members of this family are found in within O-antigen biosynthesis ...
3-339 0e+00

Glycosyltransferase WbsX; Members of this family are found in within O-antigen biosynthesis clusters in Gram negative bacteria, where they are predicted to function as glycosyltransferases.


Pssm-ID: 464132 [Multi-domain]  Cd Length: 311  Bit Score: 522.45  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456    3 IIAFYLPQFHQIKENDRWWGKGFTEWTNTKSARPLFSGHYQPREPYQNFYYDLTSPSVRKWQAEIAKAYGIYGFCYYHYW 82
Cdd:pfam14307   1 VIAFYLPQFHPIPENDEWWGKGFTEWTNVKKAKPLFKGHYQPRLPADLGYYDLRLPEVRRAQAELAKEYGIYGFCYYHYW 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456   83 FKGKRLLETPFNDVLKMKEPDFPFCLSWANEPWTKSWDGLDKHILMPQDYGDiSDWKEHFDYLLQAFQDERYIYIDDKPL 162
Cdd:pfam14307  81 FGGKRLLEKPLEELLESKEPDFPFCLCWANESWTRRWDGSDKEILIEQDYGE-EDDKAHFDYLLPYFKDPRYIKVDGKPL 159
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456  163 FIIYRPGHIPDCEKMLNYWNALARENGLKGIYFAETLNSFPL-PNINGFDASIQFepfytiahdsssdinkviydsdmql 241
Cdd:pfam14307 160 FLIYRPDLIPDPKETIERWRELAKENGLGGIYLVGVQSFGDEdPRDLGFDAAVEF------------------------- 214
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456  242 nvwDYDKVWMYILQRSLPKKKTFPGAFVDWDNTARRKNaNSSIFVGSTPEKFTIYLSKQIHRTYSFYNSE-FLFINAWNE 320
Cdd:pfam14307 215 ---DYDKLVRRILNRPPPKYKLFPGVFPGWDNTPRRGK-RGTIFHGATPEKFEKWLEKAVERAKQKPDEErIVFINAWNE 290
                         330
                  ....*....|....*....
gi 513828456  321 WAEGTYLEPDKKYGFSYLE 339
Cdd:pfam14307 291 WAEGAYLEPDLKFGYAYLE 309
RgpF COG3754
Lipopolysaccharide biosynthesis protein [Cell wall/membrane/envelope biogenesis];
1-106 5.10e-17

Lipopolysaccharide biosynthesis protein [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442968 [Multi-domain]  Cd Length: 152  Bit Score: 77.02  E-value: 5.10e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456   1 MKIIAFYLPQFHQI----------------------KENDRWWGKgfTEWTNtksaRPLFSGHYQ-----------PREP 47
Cdd:COG3754    4 VALFAFYLPQADELlltndsifgpiaplsplfdrmePENDDWWGL--GEWTN----KPLFPGHLQsyflvfrkralASPA 77
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 513828456  48 YQNFYYDLTSPS-----VRKWQAEIAKAYGIYGFCyYHYWFKGKRL----LETPFNDVLkmKEPDFPF 106
Cdd:COG3754   78 FRDFWYDLRLIEdkddvIRRYEAGLARALGIAGFC-YHYWFDGKRLrtrlLERPWDELL--ENGGFPF 142
GH99_GH71_like cd11573
Glycoside hydrolase families 71, 99, and related domains; This superfamily of glycoside ...
51-336 1.13e-14

Glycoside hydrolase families 71, 99, and related domains; This superfamily of glycoside hydrolases contains families GH71 and GH99 (following the CAZY nomenclature), as well as other members with undefined function and specificity.


Pssm-ID: 211414  Cd Length: 284  Bit Score: 73.30  E-value: 1.13e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456  51 FYYDLTSPSVRKWQAEIAKAYGIYGFCYYHYWFKGKRLLETPfNDVLKMK-----EPDFP--FCLSWANEPWTKSWDgld 123
Cdd:cd11573    3 AGYQPWTPEVMRKHIRWAQEAGIDGFAVDWYPEADTSPLAET-TAILNKAldaaeEENFTifFMLDPASLREAGELD--- 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456 124 khilmpqDYGDISDWKEHFdyllqAFQDERYIYIDDKPLFIIYRPGHI---PDCEKMLNywnalarENGLKGIYFA--ET 198
Cdd:cd11573   79 -------VVLERITRLINE-----YRNPSSYYKVGGKPLVFIWGPGLAytaSEWEALKA-------QLRAGCPYMIglWT 139
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456 199 LNSFPLPNI--NGFDASIQFEPFYTIAHDSssdinkviyDSDMQLNVWDYDKVWMYILqrslpKKKTFPGAFVDWDNTAR 276
Cdd:cd11573  140 PWRVPNRDMitDMFDGASPWTPWRGTNPEE---------AYGHGVKNWRPDQEWMGAN-----GKGYIPTVSPGFSDINR 205
                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 513828456 277 RKNANSSIFVGSTPEKFTIYLSKQIHRTysfynSEFLFINAWNEWAEGTYLEPDKKYGFS 336
Cdd:cd11573  206 RPGDPGDIILRRDGQRLHSMLEAALKAG-----PAMIQIASWNDWGEGTYIEPCEEYGPR 260
GH99_GH71_like_3 cd11575
Uncharacterized glycoside hydrolase family 99-like domain; This family of putative glycoside ...
309-339 3.69e-03

Uncharacterized glycoside hydrolase family 99-like domain; This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism.


Pssm-ID: 211416  Cd Length: 376  Bit Score: 38.86  E-value: 3.69e-03
                         10        20        30
                 ....*....|....*....|....*....|.
gi 513828456 309 NSEFLFINAWNEWAEGTYLEPDKKYGFSYLE 339
Cdd:cd11575  289 GLDIIQIATWNDYGEGTMIEPTVEFGYRDLE 319
GH99_GH71_like_1 cd11578
Uncharacterized glycoside hydrolase family 99-like domain; This family of putative glycoside ...
312-343 6.42e-03

Uncharacterized glycoside hydrolase family 99-like domain; This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism.


Pssm-ID: 211419  Cd Length: 313  Bit Score: 38.16  E-value: 6.42e-03
                         10        20        30
                 ....*....|....*....|....*....|...
gi 513828456 312 FLFINAWNEWAEGTYLEP-DKKYGFSYLEGVKN 343
Cdd:cd11578  275 IVLITSFNEWNEGTNIEPsEEAYGFGYLETLLD 307
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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