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Conserved domains on  [gi|515628111|ref|WP_017060711|]
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MULTISPECIES: arsenate reductase (glutaredoxin) [Vibrio]

Protein Classification

arsenate reductase family protein( domain architecture ID 10122547)

arsenate reductase (ArsC) family protein similar to Synechocystis sp. arsenate reductases ArsI1 and ArsI2, which are glutaredoxin-dependent enzymes that catalyze the reduction of arsenate [As(V)] to arsenite [As(III)]

PubMed:  15558583

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ArsC_ArsC cd03034
Arsenate Reductase (ArsC) family, ArsC subfamily; arsenic reductases similar to that encoded ...
 3-115 2.09e-62

Arsenate Reductase (ArsC) family, ArsC subfamily; arsenic reductases similar to that encoded by arsC on the R733 plasmid of Escherichia coli. E. coli ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], the first step in the detoxification of arsenic, using reducing equivalents derived from glutathione (GSH) via glutaredoxin (GRX). ArsC contains a single catalytic cysteine, within a thioredoxin fold, that forms a covalent thiolate-As(V) intermediate, which is reduced by GRX through a mixed GSH-arsenate intermediate. This family of predominantly bacterial enzymes is unrelated to two other families of arsenate reductases which show similarity to low-molecular-weight acid phosphatases and phosphotyrosyl phosphatases.


:

Pssm-ID: 239332  Cd Length: 112  Bit Score: 185.48  E-value: 2.09e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111   3 VVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLGfDSVRDMMRTKEADYKEANLGDASVTDEDLF 82
Cdd:cd03034    1 ITIYHNPRCSKSRNALALLEEAGIEPEIVEYLKTPPTAAELRELLAKLG-ISPRDLLRTKEAPYKELGLADPELSDEELI 79

                         90       100       110
                 ....*....|....*....|....*....|...
gi 515628111  83 AAMATNPKLFERPVVVANNKAKIGRPPEQVLEI 115
Cdd:cd03034   80 DAMAAHPILIERPIVVTGDGAVLGRPPEAVLEL 112
 
Name Accession Description Interval E-value
ArsC_ArsC cd03034
Arsenate Reductase (ArsC) family, ArsC subfamily; arsenic reductases similar to that encoded ...
 3-115 2.09e-62

Arsenate Reductase (ArsC) family, ArsC subfamily; arsenic reductases similar to that encoded by arsC on the R733 plasmid of Escherichia coli. E. coli ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], the first step in the detoxification of arsenic, using reducing equivalents derived from glutathione (GSH) via glutaredoxin (GRX). ArsC contains a single catalytic cysteine, within a thioredoxin fold, that forms a covalent thiolate-As(V) intermediate, which is reduced by GRX through a mixed GSH-arsenate intermediate. This family of predominantly bacterial enzymes is unrelated to two other families of arsenate reductases which show similarity to low-molecular-weight acid phosphatases and phosphotyrosyl phosphatases.


Pssm-ID: 239332  Cd Length: 112  Bit Score: 185.48  E-value: 2.09e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111   3 VVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLGfDSVRDMMRTKEADYKEANLGDASVTDEDLF 82
Cdd:cd03034    1 ITIYHNPRCSKSRNALALLEEAGIEPEIVEYLKTPPTAAELRELLAKLG-ISPRDLLRTKEAPYKELGLADPELSDEELI 79

                         90       100       110
                 ....*....|....*....|....*....|...
gi 515628111  83 AAMATNPKLFERPVVVANNKAKIGRPPEQVLEI 115
Cdd:cd03034   80 DAMAAHPILIERPIVVTGDGAVLGRPPEAVLEL 112
arsC TIGR00014
arsenate reductase (glutaredoxin); This model describes a distinct clade, including ArsC ...
 3-116 1.23e-51

arsenate reductase (glutaredoxin); This model describes a distinct clade, including ArsC itself, of the broader ArsC family described by Pfam pfam03960. This clade is almost completely restricted to the Proteobacteria. An anion-translocating ATPase has been identified as the product of the arsenical resistance operon of resistance plasmid R773. When expressed in Escherichia coli this ATP-driven oxyanion pump catalyses extrusion of the oxyanions arsenite, antimonite and arsenate. The pump is composed of two polypeptides, the products of the arsA and arsB genes. The pump alone produces resistance to arsenite and antimonite. This protein, ArsC, catalyzes the reduction of arsenate to arsenite, and thus extends resistance to include arsenate. [Cellular processes, Detoxification]


Pssm-ID: 272855  Cd Length: 114  Bit Score: 158.38  E-value: 1.23e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111    3 VVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLGFDSVRDMMRTKEADYKEANLGDASVTDEDLF 82
Cdd:TIGR00014   1 VTIYHNPRCSKSRNTLALLEDKGIEPEVVKYLKNPPTKSELEAIFAKLGLTVAREMIRTKEALYKELGLSDPNLSDQELL 80

                          90       100       110
                  ....*....|....*....|....*....|....
gi 515628111   83 AAMATNPKLFERPVVVANNKAKIGRPPEQVLEIL 116
Cdd:TIGR00014  81 DAMVAHPILLERPIVVAGDGARIGRPPENVLDIL 114
ArsC COG1393
Arsenate reductase or related protein, glutaredoxin family [Inorganic ion transport and ...
 2-116 1.20e-48

Arsenate reductase or related protein, glutaredoxin family [Inorganic ion transport and metabolism];


Pssm-ID: 441003  Cd Length: 115  Bit Score: 150.63  E-value: 1.20e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111   2 SVVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLGFdSVRDMMRTKEADYKEANLGDASVTDEDL 81
Cdd:COG1393    1 MITIYGNPNCSTSRKALAWLEEAGIEYEFIDYLKTPPTAEELKELLAKLGL-GVEELLNTRGTTYRELGLKDKALSEEEA 79

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 515628111  82 FAAMATNPKLFERPVVVANNKAKIGRPPEQVLEIL 116
Cdd:COG1393   80 LALMLEHPSLIKRPIVVTGDKALVGFPPEEVLALL 114
ArsC pfam03960
ArsC family; This family is related to glutaredoxins pfam00462.
 6-115 1.03e-37

ArsC family; This family is related to glutaredoxins pfam00462.


Pssm-ID: 427617  Cd Length: 109  Bit Score: 123.09  E-value: 1.03e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111    6 YHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLGfDSVRDMMRTKEADYKEANLGDASVTDEDLFAAM 85
Cdd:pfam03960   1 YGSPNCSTCRKALAWLEEHGIEYQEIDYLETPPSKEELKDILAKLG-DGVEALLNTRGTTYRELNLDKEDLSEDELLELI 79

                          90       100       110
                  ....*....|....*....|....*....|
gi 515628111   86 ATNPKLFERPVVVANNKAKIGRPPEQVLEI 115
Cdd:pfam03960  80 LEHPSLIRRPIVVDGGKLLVGFNEEEIRAF 109
PRK10026 PRK10026
arsenate reductase (glutaredoxin);
2-116 6.60e-25

arsenate reductase (glutaredoxin);


Pssm-ID: 182200  Cd Length: 141  Bit Score: 91.42  E-value: 6.60e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111   2 SVVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLGFdSVRDMMRTKEADYKEANLGDASVTDEDL 81
Cdd:PRK10026   3 NITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDELVKLIADMGI-SVRALLRKNVEPYEELGLAEDKFTDDQL 81

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 515628111  82 FAAMATNPKLFERPVVVANNKAKIGRPPEQVLEIL 116
Cdd:PRK10026  82 IDFMLQHPILINRPIVVTPLGTRLCRPSEVVLEIL 116
 
Name Accession Description Interval E-value
ArsC_ArsC cd03034
Arsenate Reductase (ArsC) family, ArsC subfamily; arsenic reductases similar to that encoded ...
 3-115 2.09e-62

Arsenate Reductase (ArsC) family, ArsC subfamily; arsenic reductases similar to that encoded by arsC on the R733 plasmid of Escherichia coli. E. coli ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], the first step in the detoxification of arsenic, using reducing equivalents derived from glutathione (GSH) via glutaredoxin (GRX). ArsC contains a single catalytic cysteine, within a thioredoxin fold, that forms a covalent thiolate-As(V) intermediate, which is reduced by GRX through a mixed GSH-arsenate intermediate. This family of predominantly bacterial enzymes is unrelated to two other families of arsenate reductases which show similarity to low-molecular-weight acid phosphatases and phosphotyrosyl phosphatases.


Pssm-ID: 239332  Cd Length: 112  Bit Score: 185.48  E-value: 2.09e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111   3 VVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLGfDSVRDMMRTKEADYKEANLGDASVTDEDLF 82
Cdd:cd03034    1 ITIYHNPRCSKSRNALALLEEAGIEPEIVEYLKTPPTAAELRELLAKLG-ISPRDLLRTKEAPYKELGLADPELSDEELI 79

                         90       100       110
                 ....*....|....*....|....*....|...
gi 515628111  83 AAMATNPKLFERPVVVANNKAKIGRPPEQVLEI 115
Cdd:cd03034   80 DAMAAHPILIERPIVVTGDGAVLGRPPEAVLEL 112
arsC TIGR00014
arsenate reductase (glutaredoxin); This model describes a distinct clade, including ArsC ...
 3-116 1.23e-51

arsenate reductase (glutaredoxin); This model describes a distinct clade, including ArsC itself, of the broader ArsC family described by Pfam pfam03960. This clade is almost completely restricted to the Proteobacteria. An anion-translocating ATPase has been identified as the product of the arsenical resistance operon of resistance plasmid R773. When expressed in Escherichia coli this ATP-driven oxyanion pump catalyses extrusion of the oxyanions arsenite, antimonite and arsenate. The pump is composed of two polypeptides, the products of the arsA and arsB genes. The pump alone produces resistance to arsenite and antimonite. This protein, ArsC, catalyzes the reduction of arsenate to arsenite, and thus extends resistance to include arsenate. [Cellular processes, Detoxification]


Pssm-ID: 272855  Cd Length: 114  Bit Score: 158.38  E-value: 1.23e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111    3 VVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLGFDSVRDMMRTKEADYKEANLGDASVTDEDLF 82
Cdd:TIGR00014   1 VTIYHNPRCSKSRNTLALLEDKGIEPEVVKYLKNPPTKSELEAIFAKLGLTVAREMIRTKEALYKELGLSDPNLSDQELL 80

                          90       100       110
                  ....*....|....*....|....*....|....
gi 515628111   83 AAMATNPKLFERPVVVANNKAKIGRPPEQVLEIL 116
Cdd:TIGR00014  81 DAMVAHPILLERPIVVAGDGARIGRPPENVLDIL 114
ArsC COG1393
Arsenate reductase or related protein, glutaredoxin family [Inorganic ion transport and ...
 2-116 1.20e-48

Arsenate reductase or related protein, glutaredoxin family [Inorganic ion transport and metabolism];


Pssm-ID: 441003  Cd Length: 115  Bit Score: 150.63  E-value: 1.20e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111   2 SVVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLGFdSVRDMMRTKEADYKEANLGDASVTDEDL 81
Cdd:COG1393    1 MITIYGNPNCSTSRKALAWLEEAGIEYEFIDYLKTPPTAEELKELLAKLGL-GVEELLNTRGTTYRELGLKDKALSEEEA 79

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 515628111  82 FAAMATNPKLFERPVVVANNKAKIGRPPEQVLEIL 116
Cdd:COG1393   80 LALMLEHPSLIKRPIVVTGDKALVGFPPEEVLALL 114
ArsC_family cd02977
Arsenate Reductase (ArsC) family; composed of TRX-fold arsenic reductases and similar proteins ...
 3-107 8.57e-41

Arsenate Reductase (ArsC) family; composed of TRX-fold arsenic reductases and similar proteins including the transcriptional regulator, Spx. ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], using reducing equivalents derived from glutathione (GSH) via glutaredoxin (GRX), through a single catalytic cysteine. This family of predominantly bacterial enzymes is unrelated to two other families of arsenate reductases which show similarity to low-molecular-weight acid phosphatases and phosphotyrosyl phosphatases. Spx is a general regulator that exerts negative and positive control over transcription initiation by binding to the C-terminal domain of the alpha subunit of RNA polymerase.


Pssm-ID: 239275  Cd Length: 105  Bit Score: 130.69  E-value: 8.57e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111   3 VVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLGfDSVRDMMRTKEADYKEANLGD-ASVTDEDL 81
Cdd:cd02977    1 ITIYGNPNCSTSRKALAWLEEHGIEYEFIDYLKEPPTKEELKELLAKLG-LGVEDLFNTRGTPYRKLGLADkDELSDEEA 79

                         90       100
                 ....*....|....*....|....*.
gi 515628111  82 FAAMATNPKLFERPVVVANNKAKIGR 107
Cdd:cd02977   80 LELMAEHPKLIKRPIVVDGDRLLVGF 105
ArsC pfam03960
ArsC family; This family is related to glutaredoxins pfam00462.
 6-115 1.03e-37

ArsC family; This family is related to glutaredoxins pfam00462.


Pssm-ID: 427617  Cd Length: 109  Bit Score: 123.09  E-value: 1.03e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111    6 YHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLGfDSVRDMMRTKEADYKEANLGDASVTDEDLFAAM 85
Cdd:pfam03960   1 YGSPNCSTCRKALAWLEEHGIEYQEIDYLETPPSKEELKDILAKLG-DGVEALLNTRGTTYRELNLDKEDLSEDELLELI 79

                          90       100       110
                  ....*....|....*....|....*....|
gi 515628111   86 ATNPKLFERPVVVANNKAKIGRPPEQVLEI 115
Cdd:pfam03960  80 LEHPSLIRRPIVVDGGKLLVGFNEEEIRAF 109
PRK10026 PRK10026
arsenate reductase (glutaredoxin);
2-116 6.60e-25

arsenate reductase (glutaredoxin);


Pssm-ID: 182200  Cd Length: 141  Bit Score: 91.42  E-value: 6.60e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111   2 SVVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLGFdSVRDMMRTKEADYKEANLGDASVTDEDL 81
Cdd:PRK10026   3 NITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDELVKLIADMGI-SVRALLRKNVEPYEELGLAEDKFTDDQL 81

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 515628111  82 FAAMATNPKLFERPVVVANNKAKIGRPPEQVLEIL 116
Cdd:PRK10026  82 IDFMLQHPILINRPIVVTPLGTRLCRPSEVVLEIL 116
ArsC_like cd03036
Arsenate Reductase (ArsC) family, unknown subfamily; uncharacterized proteins containing a ...
 3-106 6.55e-15

Arsenate Reductase (ArsC) family, unknown subfamily; uncharacterized proteins containing a CXXC motif with similarity to thioredoxin (TRX)-fold arsenic reductases, ArsC. Proteins containing a redox active CXXC motif like TRX and glutaredoxin (GRX) function as protein disulfide oxidoreductases, altering the redox state of target proteins via the reversible oxidation of the active site dithiol. ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], using reducing equivalents derived from glutathione via GRX, through a single catalytic cysteine.


Pssm-ID: 239334  Cd Length: 111  Bit Score: 64.96  E-value: 6.55e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111   3 VVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLGFDsVRDMMRTKEADYKEANLGD--ASVTDED 80
Cdd:cd03036    1 LKFYEYPKCSTCRKAKKWLDEHGVDYTAIDIVEEPPSKEELKKWLEKSGLP-LKKFFNTSGKSYRELGLKDklPSLSEEE 79

                         90       100
                 ....*....|....*....|....*.
gi 515628111  81 LFAAMATNPKLFERPVVVANNKAKIG 106
Cdd:cd03036   80 ALELLSSDGMLIKRPFVVDDDKVLVG 105
arsC_related TIGR01617
transcriptional regulator, Spx/MgsR family; This model represents a portion of the proteins ...
 5-114 5.24e-12

transcriptional regulator, Spx/MgsR family; This model represents a portion of the proteins within the larger set covered by pfam03960. That larger family includes a glutaredoxin-dependent arsenate reductase (TIGR00014). Characterized members of this family include Spx and MgsR from Bacillus subtili. Spx is a global regulator for response to thiol-specific oxidative stress. It interacts with RNA polymerase. MgsR (modulator of the general stress response, also called YqgZ) provides a second level of regulation for more than a third of the proteins in the B. subtilis general stress regulon controlled by Sigma-B. [Regulatory functions, DNA interactions]


Pssm-ID: 273720  Cd Length: 117  Bit Score: 57.83  E-value: 5.24e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111    5 IYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLGfDSVRDMMRTKEADYKEANL--GDASVTDEDLF 82
Cdd:TIGR01617   3 VYGSPNCTTCKKARRWLEANGIEYQFIDIGEDGPTREELLDILSLLE-DGIDPLLNTRGQSYRALNTsnTFLDLSDKEAL 81

                          90       100       110
                  ....*....|....*....|....*....|...
gi 515628111   83 AAMATNPKLFERPVVVANNKA-KIGRPPEQVLE 114
Cdd:TIGR01617  82 ELLAEDPALLRRPLIVDTKNRlLIGFKSESIEE 114
ArsC_Spx cd03032
Arsenate Reductase (ArsC) family, Spx subfamily; Spx is a unique RNA polymerase (RNAP)-binding ...
 3-106 8.55e-10

Arsenate Reductase (ArsC) family, Spx subfamily; Spx is a unique RNA polymerase (RNAP)-binding protein present in bacilli and some mollicutes. It inhibits transcription by binding to the C-terminal domain of the alpha subunit of RNAP, disrupting complex formation between RNAP and certain transcriptional activator proteins like ResD and ComA. In response to oxidative stress, Spx can also activate transcription, making it a general regulator that exerts both positive and negative control over transcription initiation. Spx has been shown to exert redox-sensitive transcriptional control over genes like trxA (TRX) and trxB (TRX reductase), genes that function in thiol homeostasis. This redox-sensitive activity is dependent on the presence of a CXXC motif, present in some members of the Spx subfamily, that acts as a thiol/disulfide switch. Spx has also been shown to repress genes in a sulfate-dependent manner independent of the presence of the CXXC motif.


Pssm-ID: 239330  Cd Length: 115  Bit Score: 51.86  E-value: 8.55e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111   3 VVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVL--FTQLGFDsvrDMMRTKEADYKEANLGDASVTDED 80
Cdd:cd03032    2 IKLYTSPSCSSCRKAKQWLEEHQIPFEERNLFKQPLTKEELKEIlsLTENGVE---DIISTRSKAFKNLNIDIDELSLSE 78

                         90       100
                 ....*....|....*....|....*.
gi 515628111  81 LFAAMATNPKLFERPVVVANNKAKIG 106
Cdd:cd03032   79 LIRLISEHPSLLRRPIIIDEKRLQIG 104
PRK12559 PRK12559
transcriptional regulator Spx; Provisional
 3-116 1.80e-05

transcriptional regulator Spx; Provisional


Pssm-ID: 79035  Cd Length: 131  Bit Score: 40.85  E-value: 1.80e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111   3 VVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFtQLGFDSVRDMMRTKEADYKEANLGDASVTDEDLF 82
Cdd:PRK12559   2 VVLYTTASCASCRKAKAWLEENQIDYTEKNIVSNSMTVDELKSIL-RLTEEGATEIISTRSKTFQDLNINIEELSLNEFY 80

                         90       100       110
                 ....*....|....*....|....*....|....
gi 515628111  83 AAMATNPKLFERPVVVANNKAKIGRPPEQVLEIL 116
Cdd:PRK12559  81 KLIIEHPLMLRRPIMLDEKRLQIGFNDEEIRKFL 114
spxA PRK01655
transcriptional regulator Spx; Reviewed
 3-106 2.43e-05

transcriptional regulator Spx; Reviewed


Pssm-ID: 179316  Cd Length: 131  Bit Score: 40.44  E-value: 2.43e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111   3 VVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLK--VLFTQLGFDsvrDMMRTKEADYKEANLGDASVTDED 80
Cdd:PRK01655   2 VTLFTSPSCTSCRKAKAWLEEHDIPFTERNIFSSPLTIDEIKqiLRMTEDGTD---EIISTRSKVFQKLNVDVESLSLQD 78

                         90       100
                 ....*....|....*....|....*.
gi 515628111  81 LFAAMATNPKLFERPVVVANNKAKIG 106
Cdd:PRK01655  79 LIKLISDNPGLLRRPIIIDEKRLQVG 104
ArsC_Yffb cd03035
Arsenate Reductase (ArsC) family, Yffb subfamily; Yffb is an uncharacterized bacterial protein ...
 3-106 2.91e-05

Arsenate Reductase (ArsC) family, Yffb subfamily; Yffb is an uncharacterized bacterial protein encoded by the yffb gene, related to the thioredoxin-fold arsenic reductases, ArsC. The structure of Yffb and the conservation of the catalytic cysteine suggest that it is likely to function as a glutathione (GSH)-dependent thiol reductase. ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], using reducing equivalents derived from GSH via glutaredoxin, through a single catalytic cysteine.


Pssm-ID: 239333  Cd Length: 105  Bit Score: 39.88  E-value: 2.91e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111   3 VVIYHNPRCS---KSRQTLEvlqENGVQPEVIKYLETPLTVEQLKVLFTQLGFDSV---RDMMRTKEADYKEANLGDASV 76
Cdd:cd03035    1 ITLYGIKNCDtvkKARKWLE---ARGVAYTFHDYRKDGLDAATLERWLAKVGWETLlnkRGTTWRKLDDAQKAALDAAKA 77

                         90       100       110
                 ....*....|....*....|....*....|
gi 515628111  77 TdedlfAAMATNPKLFERPVVVANNKAKIG 106
Cdd:cd03035   78 I-----ALMLEHPSLIKRPVLETGGKVLVG 102
ArsC_15kD cd03033
Arsenate Reductase (ArsC) family, 15kD protein subfamily; composed of proteins of unknown ...
 2-116 6.55e-03

Arsenate Reductase (ArsC) family, 15kD protein subfamily; composed of proteins of unknown function with similarity to thioredoxin-fold arsenic reductases, ArsC. It is encoded by an ORF present in a gene cluster associated with nitrogen fixation that also encodes dinitrogenase reductase ADP-ribosyltransferase (DRAT) and dinitrogenase reductase activating glycohydrolase (DRAG). ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], using reducing equivalents derived from glutathione via glutaredoxin, through a single catalytic cysteine.


Pssm-ID: 239331  Cd Length: 113  Bit Score: 33.79  E-value: 6.55e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515628111   2 SVVIYHNPRCSKSRQTLEVLQENGVQPEVIKYLETPLTVEQLKVLFTQLgfdSVRDMMRTKEADYKEANLGDASVTDEDL 81
Cdd:cd03033    1 DIIFYEKPGCANNARQKALLEAAGHEVEVRDLLTEPWTAETLRPFFGDL---PVAEWFNPAAPRVKSGEVVPEALDEEEA 77

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 515628111  82 FAAMATNPKLFERPVVVANNKAKIGRPPEQVLEIL 116
Cdd:cd03033   78 LALMIADPLLIRRPLMQVGDRRMVGFDTARVDAWI 112
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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