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Conserved domains on  [gi|515778391|ref|WP_017210991|]
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MULTISPECIES: Crp/Fnr family transcriptional regulator [Clostridium]

Protein Classification

Crp/Fnr family transcriptional regulator( domain architecture ID 11429533)

Crp/Fnr family transcriptional regulator containing a DNA-binding Crp-like helix-turn-helix (HTH) domain, may bind cyclic nucleotides

CATH:  2.60.120.10|1.10.10.10
Gene Ontology:  GO:0003677|GO:0030552
PubMed:  11407111|14638413

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Crp COG0664
cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal ...
 25-221 3.72e-36

cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms];


:

Pssm-ID: 440428 [Multi-domain]  Cd Length: 207  Bit Score: 126.64  E-value: 3.72e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391  25 DTILKHFQLHFYEKEEYIMKSEEKLEYYYLIVDGKIKVFYPFENGKSMLLKFYRDFNTVGDLELLKNIPILCNINAVEDT 104
Cdd:COG0664   10 EALLAHLELRTLKKGEVLFREGDPADHLYFVLSGLVKLYRISEDGREQILGFLGPGDFFGELSLLGGEPSPATAEALEDS 89

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391 105 YLIGIPSDILRENYFDNTKFLHHLVDSLSEKLYVTINNSSYNFVYPLINRLSSYLMEHIID-ESYIILNSSYLEIAEFLG 183
Cdd:COG0664   90 ELLRIPREDLEELLERNPELARALLRLLARRLRQLQERLVSLAFLSAEERLARFLLELADRlDGRIDLPLTQEEIASYLG 169

                        170       180       190
                 ....*....|....*....|....*....|....*...
gi 515778391 184 TTYRHLNRTFKELESQGIIKCENKKINILDMKKLNELS 221
Cdd:COG0664  170 LTRETVSRILKKLEKEGLIELERGRITILDREALERLA 207
 
Name Accession Description Interval E-value
Crp COG0664
cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal ...
 25-221 3.72e-36

cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms];


Pssm-ID: 440428 [Multi-domain]  Cd Length: 207  Bit Score: 126.64  E-value: 3.72e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391  25 DTILKHFQLHFYEKEEYIMKSEEKLEYYYLIVDGKIKVFYPFENGKSMLLKFYRDFNTVGDLELLKNIPILCNINAVEDT 104
Cdd:COG0664   10 EALLAHLELRTLKKGEVLFREGDPADHLYFVLSGLVKLYRISEDGREQILGFLGPGDFFGELSLLGGEPSPATAEALEDS 89

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391 105 YLIGIPSDILRENYFDNTKFLHHLVDSLSEKLYVTINNSSYNFVYPLINRLSSYLMEHIID-ESYIILNSSYLEIAEFLG 183
Cdd:COG0664   90 ELLRIPREDLEELLERNPELARALLRLLARRLRQLQERLVSLAFLSAEERLARFLLELADRlDGRIDLPLTQEEIASYLG 169

                        170       180       190
                 ....*....|....*....|....*....|....*...
gi 515778391 184 TTYRHLNRTFKELESQGIIKCENKKINILDMKKLNELS 221
Cdd:COG0664  170 LTRETVSRILKKLEKEGLIELERGRITILDREALERLA 207
PRK10402 PRK10402
DNA-binding transcriptional activator YeiL; Provisional
 1-227 1.10e-29

DNA-binding transcriptional activator YeiL; Provisional


Pssm-ID: 236682 [Multi-domain]  Cd Length: 226  Bit Score: 110.20  E-value: 1.10e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391   1 MKKILDNKLLNYYIKKHNIQSIFDDTILKHFQLHFYEKEEYIMKSEEKLEYYYLIVDGKIKVFYPFENGKSMLLKFYRDF 80
Cdd:PRK10402   1 MKESKNSEEISHYMSESAFKDCFSFDVSADTELFHFLAREYIVQEGQQPSYLFYLTRGRAKLYATLANGKVSLIDFFAAP 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391  81 NTVGDLELLKNIPILCNINAVEDTYLIGIPSDILRENYFDNTKFLHHLVDSLSEKLYVTINNSSYNFVYPLINRLSSYLM 160
Cdd:PRK10402  81 CFIGEIELIDKDHETKAVQAIEECWCLALPMKDCRPLLLNDALFLRKLCKFLSHKNYRNIVSLTQNQSFPLENRLAAFIL 160

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 515778391 161 EHIIDESYIilnSSYLEIAEFLGTTYRHLNRTFKELESQGIIKCENKKINILDMKKLNELSKNIYVK 227
Cdd:PRK10402 161 LTQEGDLYH---EKHTQAAEYLGVSYRHLLYVLAQFIQDGYLKKSKRGYLIKNRKQLSGLALELKPE 224
cNMP_binding pfam00027
Cyclic nucleotide-binding domain; This domain sensor domain can bind cAMP, cGMP, c-di-GMP, ...
 33-116 2.49e-12

Cyclic nucleotide-binding domain; This domain sensor domain can bind cAMP, cGMP, c-di-GMP, oxygen and 2-oxoglutarate (Matilla et. al., FEMS Microbiology Reviews, fuab043, 45, 2021, 1. https://doi.org/10.1093/femsre/fuab043).


Pssm-ID: 459637 [Multi-domain]  Cd Length: 89  Bit Score: 60.70  E-value: 2.49e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391   33 LHFYEKEEYIMKSEEKLEYYYLIVDGKIKVFYPFENGKSMLLKFYRDFNTVGDLELLKNIPILCNINAVEDTYLIGIPSD 112
Cdd:pfam00027   1 LRSYKAGEVIFREGDPADSLYIVLSGKVKVYRTLEDGREQILAVLGPGDFFGELALLGGEPRSATVVALTDSELLVIPRE 80


                  ....
gi 515778391  113 ILRE 116
Cdd:pfam00027  81 DFLE 84
CAP_ED cd00038
effector domain of the CAP family of transcription factors; members include CAP (or cAMP ...
 25-129 1.32e-11

effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO sensor. In all cases binding of the effector leads to conformational changes and the ability to activate transcription. Cyclic nucleotide-binding domain similar to CAP are also present in cAMP- and cGMP-dependent protein kinases (cAPK and cGPK) and vertebrate cyclic nucleotide-gated ion-channels. Cyclic nucleotide-monophosphate binding domain; proteins that bind cyclic nucleotides (cAMP or cGMP) share a structural domain of about 120 residues; the best studied is the prokaryotic catabolite gene activator, CAP, where such a domain is known to be composed of three alpha-helices and a distinctive eight-stranded, antiparallel beta-barrel structure; three conserved glycine residues are thought to be essential for maintenance of the structural integrity of the beta-barrel; CooA is a homodimeric transcription factor that belongs to CAP family; cAMP- and cGMP-dependent protein kinases (cAPK and cGPK) contain two tandem copies of the cyclic nucleotide-binding domain; cAPK's are composed of two different subunits, a catalytic chain and a regulatory chain, which contains both copies of the domain; cGPK's are single chain enzymes that include the two copies of the domain in their N-terminal section; also found in vertebrate cyclic nucleotide-gated ion-channels


Pssm-ID: 237999 [Multi-domain]  Cd Length: 115  Bit Score: 59.65  E-value: 1.32e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391  25 DTILKHFQLHFYEKEEYIMKSEEKLEYYYLIVDGKIKVFYPFENGKSMLLKFYRDFNTVGDLELLKNIPILCNINAVEDT 104
Cdd:cd00038   11 EELADALEERRFPAGEVIIRQGDPADSLYIVLSGSVEVYKLDEDGREQIVGFLGPGDLFGELALLGNGPRSATVRALTDS 90

                         90       100
                 ....*....|....*....|....*
gi 515778391 105 YLIGIPSDILRENYFDNTKFLHHLV 129
Cdd:cd00038   91 ELLVLPRSDFRRLLQEYPELARRLL 115
cNMP smart00100
Cyclic nucleotide-monophosphate binding domain; Catabolite gene activator protein (CAP) is a ...
 27-94 1.70e-06

Cyclic nucleotide-monophosphate binding domain; Catabolite gene activator protein (CAP) is a prokaryotic homologue of eukaryotic cNMP-binding domains, present in ion channels, and cNMP-dependent kinases.


Pssm-ID: 197516 [Multi-domain]  Cd Length: 120  Bit Score: 45.47  E-value: 1.70e-06
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 515778391    27 ILKHFQLHFYEKEEYIMKSEEKLEYYYLIVDGKIKVFYPFENGKSMLLKFYRDFNTVGDLELLKNIPI 94
Cdd:smart00100  13 LADALEPVRYPAGEVIIRQGDVGDSFYIIVSGEVEVYKVLEDGEEQIVGTLGPGDFFGELALLTNSRR 80
 
Name Accession Description Interval E-value
Crp COG0664
cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal ...
 25-221 3.72e-36

cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms];


Pssm-ID: 440428 [Multi-domain]  Cd Length: 207  Bit Score: 126.64  E-value: 3.72e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391  25 DTILKHFQLHFYEKEEYIMKSEEKLEYYYLIVDGKIKVFYPFENGKSMLLKFYRDFNTVGDLELLKNIPILCNINAVEDT 104
Cdd:COG0664   10 EALLAHLELRTLKKGEVLFREGDPADHLYFVLSGLVKLYRISEDGREQILGFLGPGDFFGELSLLGGEPSPATAEALEDS 89

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391 105 YLIGIPSDILRENYFDNTKFLHHLVDSLSEKLYVTINNSSYNFVYPLINRLSSYLMEHIID-ESYIILNSSYLEIAEFLG 183
Cdd:COG0664   90 ELLRIPREDLEELLERNPELARALLRLLARRLRQLQERLVSLAFLSAEERLARFLLELADRlDGRIDLPLTQEEIASYLG 169

                        170       180       190
                 ....*....|....*....|....*....|....*...
gi 515778391 184 TTYRHLNRTFKELESQGIIKCENKKINILDMKKLNELS 221
Cdd:COG0664  170 LTRETVSRILKKLEKEGLIELERGRITILDREALERLA 207
PRK10402 PRK10402
DNA-binding transcriptional activator YeiL; Provisional
 1-227 1.10e-29

DNA-binding transcriptional activator YeiL; Provisional


Pssm-ID: 236682 [Multi-domain]  Cd Length: 226  Bit Score: 110.20  E-value: 1.10e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391   1 MKKILDNKLLNYYIKKHNIQSIFDDTILKHFQLHFYEKEEYIMKSEEKLEYYYLIVDGKIKVFYPFENGKSMLLKFYRDF 80
Cdd:PRK10402   1 MKESKNSEEISHYMSESAFKDCFSFDVSADTELFHFLAREYIVQEGQQPSYLFYLTRGRAKLYATLANGKVSLIDFFAAP 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391  81 NTVGDLELLKNIPILCNINAVEDTYLIGIPSDILRENYFDNTKFLHHLVDSLSEKLYVTINNSSYNFVYPLINRLSSYLM 160
Cdd:PRK10402  81 CFIGEIELIDKDHETKAVQAIEECWCLALPMKDCRPLLLNDALFLRKLCKFLSHKNYRNIVSLTQNQSFPLENRLAAFIL 160

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 515778391 161 EHIIDESYIilnSSYLEIAEFLGTTYRHLNRTFKELESQGIIKCENKKINILDMKKLNELSKNIYVK 227
Cdd:PRK10402 161 LTQEGDLYH---EKHTQAAEYLGVSYRHLLYVLAQFIQDGYLKKSKRGYLIKNRKQLSGLALELKPE 224
cNMP_binding pfam00027
Cyclic nucleotide-binding domain; This domain sensor domain can bind cAMP, cGMP, c-di-GMP, ...
 33-116 2.49e-12

Cyclic nucleotide-binding domain; This domain sensor domain can bind cAMP, cGMP, c-di-GMP, oxygen and 2-oxoglutarate (Matilla et. al., FEMS Microbiology Reviews, fuab043, 45, 2021, 1. https://doi.org/10.1093/femsre/fuab043).


Pssm-ID: 459637 [Multi-domain]  Cd Length: 89  Bit Score: 60.70  E-value: 2.49e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391   33 LHFYEKEEYIMKSEEKLEYYYLIVDGKIKVFYPFENGKSMLLKFYRDFNTVGDLELLKNIPILCNINAVEDTYLIGIPSD 112
Cdd:pfam00027   1 LRSYKAGEVIFREGDPADSLYIVLSGKVKVYRTLEDGREQILAVLGPGDFFGELALLGGEPRSATVVALTDSELLVIPRE 80


                  ....
gi 515778391  113 ILRE 116
Cdd:pfam00027  81 DFLE 84
HTH_Crp_2 pfam13545
Crp-like helix-turn-helix domain; This family represents a crp-like helix-turn-helix domain ...
 154-220 3.83e-12

Crp-like helix-turn-helix domain; This family represents a crp-like helix-turn-helix domain that is likely to bind DNA.


Pssm-ID: 463917 [Multi-domain]  Cd Length: 68  Bit Score: 59.78  E-value: 3.83e-12
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 515778391  154 RLSSYLMEHIIDES--YIILNSSYLEIAEFLGTTYRHLNRTFKELESQGIIkcENKKINILDMKKLNEL 220
Cdd:pfam13545   2 RLARFLLELAARDGggRIDLPLTQEDLADLLGTTRETVSRVLSELRREGLI--ERGRITILDPEALEAL 68
CAP_ED cd00038
effector domain of the CAP family of transcription factors; members include CAP (or cAMP ...
 25-129 1.32e-11

effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO sensor. In all cases binding of the effector leads to conformational changes and the ability to activate transcription. Cyclic nucleotide-binding domain similar to CAP are also present in cAMP- and cGMP-dependent protein kinases (cAPK and cGPK) and vertebrate cyclic nucleotide-gated ion-channels. Cyclic nucleotide-monophosphate binding domain; proteins that bind cyclic nucleotides (cAMP or cGMP) share a structural domain of about 120 residues; the best studied is the prokaryotic catabolite gene activator, CAP, where such a domain is known to be composed of three alpha-helices and a distinctive eight-stranded, antiparallel beta-barrel structure; three conserved glycine residues are thought to be essential for maintenance of the structural integrity of the beta-barrel; CooA is a homodimeric transcription factor that belongs to CAP family; cAMP- and cGMP-dependent protein kinases (cAPK and cGPK) contain two tandem copies of the cyclic nucleotide-binding domain; cAPK's are composed of two different subunits, a catalytic chain and a regulatory chain, which contains both copies of the domain; cGPK's are single chain enzymes that include the two copies of the domain in their N-terminal section; also found in vertebrate cyclic nucleotide-gated ion-channels


Pssm-ID: 237999 [Multi-domain]  Cd Length: 115  Bit Score: 59.65  E-value: 1.32e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391  25 DTILKHFQLHFYEKEEYIMKSEEKLEYYYLIVDGKIKVFYPFENGKSMLLKFYRDFNTVGDLELLKNIPILCNINAVEDT 104
Cdd:cd00038   11 EELADALEERRFPAGEVIIRQGDPADSLYIVLSGSVEVYKLDEDGREQIVGFLGPGDLFGELALLGNGPRSATVRALTDS 90

                         90       100
                 ....*....|....*....|....*
gi 515778391 105 YLIGIPSDILRENYFDNTKFLHHLV 129
Cdd:cd00038   91 ELLVLPRSDFRRLLQEYPELARRLL 115
cNMP smart00100
Cyclic nucleotide-monophosphate binding domain; Catabolite gene activator protein (CAP) is a ...
 27-94 1.70e-06

Cyclic nucleotide-monophosphate binding domain; Catabolite gene activator protein (CAP) is a prokaryotic homologue of eukaryotic cNMP-binding domains, present in ion channels, and cNMP-dependent kinases.


Pssm-ID: 197516 [Multi-domain]  Cd Length: 120  Bit Score: 45.47  E-value: 1.70e-06
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 515778391    27 ILKHFQLHFYEKEEYIMKSEEKLEYYYLIVDGKIKVFYPFENGKSMLLKFYRDFNTVGDLELLKNIPI 94
Cdd:smart00100  13 LADALEPVRYPAGEVIIRQGDVGDSFYIIVSGEVEVYKVLEDGEEQIVGTLGPGDFFGELALLTNSRR 80
HTH_CRP smart00419
helix_turn_helix, cAMP Regulatory protein;
177-212 1.73e-05

helix_turn_helix, cAMP Regulatory protein;


Pssm-ID: 128696 [Multi-domain]  Cd Length: 48  Bit Score: 40.89  E-value: 1.73e-05
                           10        20        30
                   ....*....|....*....|....*....|....*.
gi 515778391   177 EIAEFLGTTYRHLNRTFKELESQGIIKCENKKINIL 212
Cdd:smart00419  13 EIAELLGLTRETVSRTLKRLEKEGLISREGGRIVIL 48
HTH_CRP cd00092
helix_turn_helix, cAMP Regulatory protein C-terminus; DNA binding domain of prokaryotic ...
 152-208 1.85e-05

helix_turn_helix, cAMP Regulatory protein C-terminus; DNA binding domain of prokaryotic regulatory proteins belonging to the catabolite activator protein family.


Pssm-ID: 238044 [Multi-domain]  Cd Length: 67  Bit Score: 41.50  E-value: 1.85e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 515778391 152 INRLSSYLMEHIIDESYIILNSSYL---EIAEFLGTTYRHLNRTFKELESQGIIKCENKK 208
Cdd:cd00092    2 KERLASFLLNLSLRYGAGDLVQLPLtrqEIADYLGLTRETVSRTLKELEEEGLISRRGRG 61
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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