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Conserved domains on  [gi|515953441|ref|WP_017384024|]
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MULTISPECIES: G/U mismatch-specific DNA glycosylase [Enterobacter]

Protein Classification

mismatch-specific DNA-glycosylase( domain architecture ID 10793356)

mismatch-specific DNA-glycosylase repairs a variety of DNA lesions, such as caused by G:T and G:U mismatches, in the base-excision repair pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK10201 PRK10201
G/U mismatch-specific DNA glycosylase;
1-168 1.47e-135

G/U mismatch-specific DNA glycosylase;


:

Pssm-ID: 182301  Cd Length: 168  Bit Score: 374.85  E-value: 1.47e-135
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441   1 MINDILAPGLRVVFCGINPGKSSAHTGFHFAHPGNRFWKVIHQAGFTDKQLKPEEEQQLLDTRCGITMLVERPTVQASEV 80
Cdd:PRK10201   1 MVEDILAPGLRVVFCGINPGLSSAHTGFPFAHPANRFWKVIHQAGFTDRQLKPEEAQHLLDTRCGVTKLVDRPTVQANEV 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441  81 NLHELRAGGRELIAKIEDYQPAALAILGKQAYEQAFSQRGASWGKQNITIGVTQVWVLPNPSGLNRATLDKLVEAYRELD 160
Cdd:PRK10201  81 SKQELRSGGRKLIEKIEDYQPQALAVLGKQAYEQGFSQRGAQWGKQTLTIGSTQVWVLPNPSGLNRASLEKLVEAYRELD 160


                 ....*...
gi 515953441 161 EALVVRGL 168
Cdd:PRK10201 161 QALVVRGR 168
 
Name Accession Description Interval E-value
PRK10201 PRK10201
G/U mismatch-specific DNA glycosylase;
1-168 1.47e-135

G/U mismatch-specific DNA glycosylase;


Pssm-ID: 182301  Cd Length: 168  Bit Score: 374.85  E-value: 1.47e-135
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441   1 MINDILAPGLRVVFCGINPGKSSAHTGFHFAHPGNRFWKVIHQAGFTDKQLKPEEEQQLLDTRCGITMLVERPTVQASEV 80
Cdd:PRK10201   1 MVEDILAPGLRVVFCGINPGLSSAHTGFPFAHPANRFWKVIHQAGFTDRQLKPEEAQHLLDTRCGVTKLVDRPTVQANEV 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441  81 NLHELRAGGRELIAKIEDYQPAALAILGKQAYEQAFSQRGASWGKQNITIGVTQVWVLPNPSGLNRATLDKLVEAYRELD 160
Cdd:PRK10201  81 SKQELRSGGRKLIEKIEDYQPQALAVLGKQAYEQGFSQRGAQWGKQTLTIGSTQVWVLPNPSGLNRASLEKLVEAYRELD 160


                 ....*...
gi 515953441 161 EALVVRGL 168
Cdd:PRK10201 161 QALVVRGR 168
UDG-F2_TDG_MUG cd10028
Uracil DNA glycosylase family 2, includes thymine DNA glycosylase, mismatch-specific uracil ...
 2-159 2.48e-78

Uracil DNA glycosylase family 2, includes thymine DNA glycosylase, mismatch-specific uracil DNA glycosylase and similar proteins; Uracil DNA glycosylase family 2 consists of thymine DNA glycosylase (TDG), which removes uracil and thymine from G:U and G:T mismatches in double-stranded DNA. It includes mismatch-specific uracil DNA glycosylase (MUG), the prokaryotic homolog of TDG. Escherichia coli MUG is highly specific to G:U mismatches but also repairs G:T mismatches at high enzyme concentration. Uracil-DNA glycosylases (UDGs) initiate repair of uracils in DNA. Uracil in DNA can arise as a result of misincorporation of dUMP residues by DNA polymerase or via deamination of cytosine. Uracil in DNA mispaired with guanine is one of the major pro-mutagenic events, causing G:C->A:T mutations. Thus, UDG is an essential enzyme for maintaining the integrity of genetic information. UDGs have been classified into various families on the basis of their substrate specificity, conserved motifs, and structural similarities. Although these families demonstrate different substrate specificities, often the function of one enzyme can be complemented by the other..


Pssm-ID: 381679  Cd Length: 163  Bit Score: 230.06  E-value: 2.48e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441   2 INDILAPGLRVVFCGINPGKSSAHTGFHFAHPGNRFWKVIHQAGFTDKQLKPEEEQQLLDTRCGITMLVERPTVQASEVN 81
Cdd:cd10028    1 LPDLLAPGLDVLFCGINPGLRSAAVGHHYAHPGNRFWPLLHESGLTPRLLTPEEDRRLPEYGIGLTNLVKRPTASAAELS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441  82 LHELRAGGRELIAKIEDYQPAALAILGKQAYEQAFSQ--RGASWGKQNIT-IGVTQVWVLPNPSGLNRA-TLDKLVEAYR 157
Cdd:cd10028   81 KAELRAGVPRLLAKIARYRPRVVAFVGKGAYRAFFGRlkKKAAYGLQPETgIGGTRVFVLPSTSGLNAHySLEDKLEPWR 160


                 ..
gi 515953441 158 EL 159
Cdd:cd10028  161 EL 162
Mug COG3663
G:T/U-mismatch repair DNA glycosylase [Replication, recombination and repair];
4-159 1.32e-62

G:T/U-mismatch repair DNA glycosylase [Replication, recombination and repair];


Pssm-ID: 442880  Cd Length: 157  Bit Score: 189.99  E-value: 1.32e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441   4 DILAPGLRVVFCGINPGKSSAHTGFHFAHPGNRFWKVIHQAGFTDKQL-KPEEEQQLLDTRCGITMLVERPTVQASEVNL 82
Cdd:COG3663    3 PVLAPGLRVLILGSNPGLASLAAGFYYAHPRNRFWPILGAAGGTDPRLdYPERKAFLLEHGIGLWDVVARCTRRAGSLDS 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441  83 HELRAGGRELIAkIEDYQPA--ALAILGKQAYEQAFSQRgaswGKQNITIGVTQVWVLPNPSGLNRA-TLDKLVEAYREL 159
Cdd:COG3663   83 AIRNAGPNDLAA-LLRYRPRikTVAFNGKTAYRLFFKLV----APQPETIGGIELWVLPSPSPANARfSLEEKLAAWREL 157
UDG pfam03167
Uracil DNA glycosylase superfamily;
4-159 2.65e-28

Uracil DNA glycosylase superfamily;


Pssm-ID: 397331  Cd Length: 154  Bit Score: 102.42  E-value: 2.65e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441    4 DILAPGLRVVFCGINPGKSSAHTGFHF-AHPGNRFWKVIHQAGFTDKQLKPEeeqqlldtRCGITMLV-ERPTvQASEVN 81
Cdd:pfam03167   2 GFGPPNAKVLIVGEAPGADEDATGLPFvGRAGNLLWKLLNAAGLTRDLFSPQ--------GVYITNVVkCRPG-NRRKPT 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441   82 LHELRAGGRELIAKIEDYQPAALAILGKQAYeQAFSQRGASWG--KQNITIGVTQVWVLPNPSGLNRATLD--KLVEAYR 157
Cdd:pfam03167  73 SHEIDACWPYLEAEIELLRPRVIVLLGKTAA-KALLGLKKITKlrGKLIDLKGIPVLPTPHPSPLLRNKLNpfLKANAWE 151


                  ..
gi 515953441  158 EL 159
Cdd:pfam03167 152 DL 153
mug TIGR00584
mismatch-specific thymine-DNA glycosylate (mug); All proteins in this family for whcih ...
 4-147 2.38e-22

mismatch-specific thymine-DNA glycosylate (mug); All proteins in this family for whcih functions are known are G-T or G-U mismatch glycosylases that function in base excision repair. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). Used 2pf model. [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273154  Cd Length: 328  Bit Score: 90.88  E-value: 2.38e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441    4 DILAPGLRVVFCGINPGKSSAHTGFHFAHPGNRFWKVIHQAGFTDKQLKPEEEQQLLDTR-CGITMLVERPTVQASEVNL 82
Cdd:TIGR00584 125 DILTFNLDIVIIGINPGLMAAYKGHHYPGPGNHFWKCLFMSGLSEVQLNHMDDHTLPGKYgIGFTNMVERTTPGSKDLSS 204

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 515953441   83 HELRAGGRELIAKIEDYQPAALAILGKQAYEQAFSQ------RGASWGKQNITIGVTQVWVLPNPSGLNRA 147
Cdd:TIGR00584 205 KEFREGGRILVQKLQKYQPRIAVFNGKCIYEIFSKEvfgvkvKNLEFGLQPHKIPDTETLCYVMPSSSARC 275
UDG smart00986
Uracil DNA glycosylase superfamily;
4-147 9.93e-14

Uracil DNA glycosylase superfamily;


Pssm-ID: 214956  Cd Length: 156  Bit Score: 64.72  E-value: 9.93e-14
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441     4 DILAPGLRVVFCGINPGKSSAH-TGFHFAHPGNRFWKVIHQAGFTDKQLkpeeeqqLLDTRCGITMLVERPTVQAS-EVN 81
Cdd:smart00986   2 GTGDPNAKVLIVGQAPGASEEDrGGPFVGAAGLLLSVMLGVAGLPRLPP-------YLTNIVKCRPPDAGNRRPTSwELQ 74

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 515953441    82 LHELRAGGRElIAKIedyQPAALAILGKQAYEQAFSQRGAS-----WGKQ-NITIGVTQVWVLPNPSGLNRA 147
Cdd:smart00986  75 GCLLPWLTVE-LALA---RPHLILLLGKFAAQALLGLLRRPlvfglRGRVaQLKGKGHRVLPLPHPSPLNRN 142
 
Name Accession Description Interval E-value
PRK10201 PRK10201
G/U mismatch-specific DNA glycosylase;
1-168 1.47e-135

G/U mismatch-specific DNA glycosylase;


Pssm-ID: 182301  Cd Length: 168  Bit Score: 374.85  E-value: 1.47e-135
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441   1 MINDILAPGLRVVFCGINPGKSSAHTGFHFAHPGNRFWKVIHQAGFTDKQLKPEEEQQLLDTRCGITMLVERPTVQASEV 80
Cdd:PRK10201   1 MVEDILAPGLRVVFCGINPGLSSAHTGFPFAHPANRFWKVIHQAGFTDRQLKPEEAQHLLDTRCGVTKLVDRPTVQANEV 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441  81 NLHELRAGGRELIAKIEDYQPAALAILGKQAYEQAFSQRGASWGKQNITIGVTQVWVLPNPSGLNRATLDKLVEAYRELD 160
Cdd:PRK10201  81 SKQELRSGGRKLIEKIEDYQPQALAVLGKQAYEQGFSQRGAQWGKQTLTIGSTQVWVLPNPSGLNRASLEKLVEAYRELD 160


                 ....*...
gi 515953441 161 EALVVRGL 168
Cdd:PRK10201 161 QALVVRGR 168
UDG-F2_TDG_MUG cd10028
Uracil DNA glycosylase family 2, includes thymine DNA glycosylase, mismatch-specific uracil ...
 2-159 2.48e-78

Uracil DNA glycosylase family 2, includes thymine DNA glycosylase, mismatch-specific uracil DNA glycosylase and similar proteins; Uracil DNA glycosylase family 2 consists of thymine DNA glycosylase (TDG), which removes uracil and thymine from G:U and G:T mismatches in double-stranded DNA. It includes mismatch-specific uracil DNA glycosylase (MUG), the prokaryotic homolog of TDG. Escherichia coli MUG is highly specific to G:U mismatches but also repairs G:T mismatches at high enzyme concentration. Uracil-DNA glycosylases (UDGs) initiate repair of uracils in DNA. Uracil in DNA can arise as a result of misincorporation of dUMP residues by DNA polymerase or via deamination of cytosine. Uracil in DNA mispaired with guanine is one of the major pro-mutagenic events, causing G:C->A:T mutations. Thus, UDG is an essential enzyme for maintaining the integrity of genetic information. UDGs have been classified into various families on the basis of their substrate specificity, conserved motifs, and structural similarities. Although these families demonstrate different substrate specificities, often the function of one enzyme can be complemented by the other..


Pssm-ID: 381679  Cd Length: 163  Bit Score: 230.06  E-value: 2.48e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441   2 INDILAPGLRVVFCGINPGKSSAHTGFHFAHPGNRFWKVIHQAGFTDKQLKPEEEQQLLDTRCGITMLVERPTVQASEVN 81
Cdd:cd10028    1 LPDLLAPGLDVLFCGINPGLRSAAVGHHYAHPGNRFWPLLHESGLTPRLLTPEEDRRLPEYGIGLTNLVKRPTASAAELS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441  82 LHELRAGGRELIAKIEDYQPAALAILGKQAYEQAFSQ--RGASWGKQNIT-IGVTQVWVLPNPSGLNRA-TLDKLVEAYR 157
Cdd:cd10028   81 KAELRAGVPRLLAKIARYRPRVVAFVGKGAYRAFFGRlkKKAAYGLQPETgIGGTRVFVLPSTSGLNAHySLEDKLEPWR 160


                 ..
gi 515953441 158 EL 159
Cdd:cd10028  161 EL 162
Mug COG3663
G:T/U-mismatch repair DNA glycosylase [Replication, recombination and repair];
4-159 1.32e-62

G:T/U-mismatch repair DNA glycosylase [Replication, recombination and repair];


Pssm-ID: 442880  Cd Length: 157  Bit Score: 189.99  E-value: 1.32e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441   4 DILAPGLRVVFCGINPGKSSAHTGFHFAHPGNRFWKVIHQAGFTDKQL-KPEEEQQLLDTRCGITMLVERPTVQASEVNL 82
Cdd:COG3663    3 PVLAPGLRVLILGSNPGLASLAAGFYYAHPRNRFWPILGAAGGTDPRLdYPERKAFLLEHGIGLWDVVARCTRRAGSLDS 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441  83 HELRAGGRELIAkIEDYQPA--ALAILGKQAYEQAFSQRgaswGKQNITIGVTQVWVLPNPSGLNRA-TLDKLVEAYREL 159
Cdd:COG3663   83 AIRNAGPNDLAA-LLRYRPRikTVAFNGKTAYRLFFKLV----APQPETIGGIELWVLPSPSPANARfSLEEKLAAWREL 157
UDG-like cd09593
uracil-DNA glycosylases (UDG) and related enzymes; Uracil-DNA glycosylases (UDGs) initiate ...
 12-146 4.00e-32

uracil-DNA glycosylases (UDG) and related enzymes; Uracil-DNA glycosylases (UDGs) initiate repair of uracils in DNA. Uracil may arise from misincorporation of dUMP residues by DNA polymerase or via deamination of cytosine. Uracil in DNA mispaired with guanine is one of the major pro-mutagenic events, causing G:C->A:T mutations; thus, UDG is an essential enzyme for maintaining the integrity of genetic information. UDGs have been classified into various families on the basis of their substrate specificity, conserved motifs, and structural similarities. Although these families demonstrate different substrate specificities, often the function of one enzyme can be complemented by the other. UDG family 1 is the most efficient uracil-DNA glycosylase (UDG, also known as UNG) and shows a specificity for uracil in DNA. UDG family 2 includes thymine DNA glycosylase which removes uracil and thymine from G:U and G:T mismatches, and mismatch-specific uracil DNA glycosylase (MUG) which in Escherichia coli is highly specific to G:U mismatches, but also repairs G:T mismatches at high enzyme concentration. UDG family 3 includes Human SMUG1 which can remove uracil and its oxidized pyrimidine derivatives from, single-stranded DNA and double-stranded DNA with a preference for single-stranded DNA. Pedobacter heparinus SMUG2, which is UDG family 3 SMUG1-like, displays catalytic activities towards DNA containing uracil or hypoxanthine/xanthine. UDG family 4 includes Thermotoga maritima TTUDGA, a robust UDG which like family 1, acts on double-stranded and single-stranded uracil-containing DNA. UDG family 5 (UDGb) includes Thermus thermophilus HB8 TTUDGB which acts on double-stranded uracil-containing DNA; it is a hypoxanthine DNA glycosylase acting on double-stranded hypoxanthine-containing DNA except for the C/I base pair, as well as a xanthine DNA glycosylase which acts on both double-stranded and single-stranded xanthine-containing DNA. UDG family 6 hypoxanthine-DNA glycosylase lacks any detectable UDG activity; it excises hypoxanthine. Other UDG families include one represented by Bradyrhizobium diazoefficiens Blr0248 which prefers single-stranded DNA and removes uracil, 5-hydroxymethyl-uracil or xanthine from it.


Pssm-ID: 381677  Cd Length: 125  Bit Score: 111.33  E-value: 4.00e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441  12 VVFCGINPG-KSSAHTGFHFAHPGNRFWKVIHQAGFTDKqlkpeeeqqLLDTRCGITMLVERPTVQASEVNLH-ELRAGG 89
Cdd:cd09593    1 VLIVGQNPGpHGARAGGVPPGPSGNRLWRLLAAAGGTPR---------LFRYGVGLTNTVPRGPPGAAAGSEKkELRFCG 71

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 515953441  90 RELIAKIEDYQPAALAILGKQAYEQAFSQRGASWGkqnITIGVTQVWVLPNPSGLNR 146
Cdd:cd09593   72 RWLRKLLELLNPRVVVLLGKKAQEAYLAVLTSSKG---APGKGTEVLVLPHPSPRNR 125
UDG pfam03167
Uracil DNA glycosylase superfamily;
4-159 2.65e-28

Uracil DNA glycosylase superfamily;


Pssm-ID: 397331  Cd Length: 154  Bit Score: 102.42  E-value: 2.65e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441    4 DILAPGLRVVFCGINPGKSSAHTGFHF-AHPGNRFWKVIHQAGFTDKQLKPEeeqqlldtRCGITMLV-ERPTvQASEVN 81
Cdd:pfam03167   2 GFGPPNAKVLIVGEAPGADEDATGLPFvGRAGNLLWKLLNAAGLTRDLFSPQ--------GVYITNVVkCRPG-NRRKPT 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441   82 LHELRAGGRELIAKIEDYQPAALAILGKQAYeQAFSQRGASWG--KQNITIGVTQVWVLPNPSGLNRATLD--KLVEAYR 157
Cdd:pfam03167  73 SHEIDACWPYLEAEIELLRPRVIVLLGKTAA-KALLGLKKITKlrGKLIDLKGIPVLPTPHPSPLLRNKLNpfLKANAWE 151


                  ..
gi 515953441  158 EL 159
Cdd:pfam03167 152 DL 153
mug TIGR00584
mismatch-specific thymine-DNA glycosylate (mug); All proteins in this family for whcih ...
 4-147 2.38e-22

mismatch-specific thymine-DNA glycosylate (mug); All proteins in this family for whcih functions are known are G-T or G-U mismatch glycosylases that function in base excision repair. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). Used 2pf model. [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273154  Cd Length: 328  Bit Score: 90.88  E-value: 2.38e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441    4 DILAPGLRVVFCGINPGKSSAHTGFHFAHPGNRFWKVIHQAGFTDKQLKPEEEQQLLDTR-CGITMLVERPTVQASEVNL 82
Cdd:TIGR00584 125 DILTFNLDIVIIGINPGLMAAYKGHHYPGPGNHFWKCLFMSGLSEVQLNHMDDHTLPGKYgIGFTNMVERTTPGSKDLSS 204

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 515953441   83 HELRAGGRELIAKIEDYQPAALAILGKQAYEQAFSQ------RGASWGKQNITIGVTQVWVLPNPSGLNRA 147
Cdd:TIGR00584 205 KEFREGGRILVQKLQKYQPRIAVFNGKCIYEIFSKEvfgvkvKNLEFGLQPHKIPDTETLCYVMPSSSARC 275
UDG smart00986
Uracil DNA glycosylase superfamily;
4-147 9.93e-14

Uracil DNA glycosylase superfamily;


Pssm-ID: 214956  Cd Length: 156  Bit Score: 64.72  E-value: 9.93e-14
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515953441     4 DILAPGLRVVFCGINPGKSSAH-TGFHFAHPGNRFWKVIHQAGFTDKQLkpeeeqqLLDTRCGITMLVERPTVQAS-EVN 81
Cdd:smart00986   2 GTGDPNAKVLIVGQAPGASEEDrGGPFVGAAGLLLSVMLGVAGLPRLPP-------YLTNIVKCRPPDAGNRRPTSwELQ 74

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 515953441    82 LHELRAGGRElIAKIedyQPAALAILGKQAYEQAFSQRGAS-----WGKQ-NITIGVTQVWVLPNPSGLNRA 147
Cdd:smart00986  75 GCLLPWLTVE-LALA---RPHLILLLGKFAAQALLGLLRRPlvfglRGRVaQLKGKGHRVLPLPHPSPLNRN 142
UDG-F6_HDG cd10032
Uracil DNA glycosylase family 6, includes hypoxanthine-DNA glycosylase and similar proteins; ...
 11-66 2.15e-08

Uracil DNA glycosylase family 6, includes hypoxanthine-DNA glycosylase and similar proteins; Uracil DNA glycosylase family 6 hypoxanthine-DNA glycosylase (HDG) lacks any detectable UDG activity; it excises hypoxanthine, a deamination product of adenine, from double-stranded DNA. Uracil-DNA glycosylase (UDGs) initiates repair of uracils in DNA. Uracil in DNA can arise as a result of misincorporation of dUMP residues by DNA polymerase or via deamination of cytosine. Uracil in DNA mispaired with guanine is one of the major pro-mutagenic events, causing G:C->A:T mutations. Thus, UDG is an essential enzyme for maintaining the integrity of genetic information. UDGs have been classified into various families on the basis of their substrate specificity, conserved motifs, and structural similarities. Although these families demonstrate different substrate specificities, often the function of one enzyme can be complemented by the other.


Pssm-ID: 381682  Cd Length: 141  Bit Score: 50.27  E-value: 2.15e-08
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 515953441  11 RVVFCGINPGKSSAHTGFHFAHPGNRFWKVIHQAGFTDKQLKPEEE--QQLLDTRCGI 66
Cdd:cd10032   12 RVLILGSFPSVKSLEAQFYYGHPRNRFWRILGALFGEDLKALDYEErkAFLLEHGIAL 69
hypoxanDNAglyco TIGR04274
hypoxanthine-DNA glycosylase; Members of this protein family represent family 6 of the ...
 5-66 1.37e-03

hypoxanthine-DNA glycosylase; Members of this protein family represent family 6 of the uracil-DNA glycosylase superfamily, where the five previously described families all act as uracil-DNA glycosylase (EC 3.2.2.27) per se. This family, instead, acts as a hypoxanthine-DNA glycosylase, where hypoxanthine results from deamination of adenine. Activity was shown directly for members from Methanosarcina barkeri and Methanosarcina acetivorans.


Pssm-ID: 211997  Cd Length: 150  Bit Score: 37.36  E-value: 1.37e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 515953441    5 ILAPGLRVVFCGINPGKSSAHTGFHFAHPGNRFWKVIHQAGFTDKQLKPEEEQQLLdTRCGI 66
Cdd:TIGR04274   5 VLPSDPRILILGSLPGNLSLRKQQYYAHPGNQFWRIIESLIGEPHSLNYPTRIELL-KRHGI 65
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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