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Conserved domains on  [gi|515972316|ref|WP_017402899|]
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MULTISPECIES: GNAT family N-acetyltransferase [Acinetobacter]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10456837)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  15581578|10940244|9175471|27367672|26818562
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 47-134 2.88e-17

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


:

Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 72.55  E-value: 2.88e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515972316   47 FDENEPMYCAVAKQGEQVLGIVHYVIHrstWAKNNYCYLEDLYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWHTQET 126
Cdd:pfam00583  27 WDEDASEGFFVAEEDGELVGFASLSII---DDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAAD 103


                  ....*...
gi 515972316  127 NVTAQKLY 134
Cdd:pfam00583 104 NLAAIALY 111
 
Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 47-134 2.88e-17

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 72.55  E-value: 2.88e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515972316   47 FDENEPMYCAVAKQGEQVLGIVHYVIHrstWAKNNYCYLEDLYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWHTQET 126
Cdd:pfam00583  27 WDEDASEGFFVAEEDGELVGFASLSII---DDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAAD 103


                  ....*...
gi 515972316  127 NVTAQKLY 134
Cdd:pfam00583 104 NLAAIALY 111
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
43-134 7.68e-17

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 72.72  E-value: 7.68e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515972316  43 WERFFDENEP--MYCAVAKQGEQVLGIVHYVIHRsTWAKNNYCYLEDLYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLY 120
Cdd:COG1247   40 REAWFAAILApgRPVLVAEEDGEVVGFASLGPFR-PRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLV 118

                         90
                 ....*....|....
gi 515972316 121 WHTQETNVTAQKLY 134
Cdd:COG1247  119 AVVLADNEASIALY 132
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 55-122 2.51e-13

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 60.75  E-value: 2.51e-13
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 515972316  55 CAVAKQGEQVLGIVHYVIHrstWAKNNYCYLEDLYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWH 122
Cdd:cd04301    1 FLVAEDDGEIVGFASLSPD---GSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
PRK10975 PRK10975
dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;
88-134 1.44e-04

dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;


Pssm-ID: 182877  Cd Length: 194  Bit Score: 39.91  E-value: 1.44e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 515972316  88 LYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWHTQETNVTAQKLY 134
Cdd:PRK10975 132 LAVFPGAQGRGIGARLMQAALNWCQARGLTRLRVATQMGNLAALRLY 178
 
Name Accession Description Interval E-value
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 47-134 2.88e-17

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 72.55  E-value: 2.88e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515972316   47 FDENEPMYCAVAKQGEQVLGIVHYVIHrstWAKNNYCYLEDLYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWHTQET 126
Cdd:pfam00583  27 WDEDASEGFFVAEEDGELVGFASLSII---DDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVAAD 103


                  ....*...
gi 515972316  127 NVTAQKLY 134
Cdd:pfam00583 104 NLAAIALY 111
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
43-134 7.68e-17

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 72.72  E-value: 7.68e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515972316  43 WERFFDENEP--MYCAVAKQGEQVLGIVHYVIHRsTWAKNNYCYLEDLYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLY 120
Cdd:COG1247   40 REAWFAAILApgRPVLVAEEDGEVVGFASLGPFR-PRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARGYRRLV 118

                         90
                 ....*....|....
gi 515972316 121 WHTQETNVTAQKLY 134
Cdd:COG1247  119 AVVLADNEASIALY 132
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 66-134 3.94e-16

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 68.91  E-value: 3.94e-16
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 515972316  66 GIVHYVIHRSTwaknNYCYLEDLYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWHTQETNVTAQKLY 134
Cdd:COG0456    1 GFALLGLVDGG----DEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALY 65
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 43-134 3.89e-14

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 64.63  E-value: 3.89e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515972316  43 WERFFDENEPMYCAVAKQGEQVLGIVHYVIHrstwaKNNYCYLEDLYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWh 122
Cdd:COG1246   18 IRPYALEEEIGEFWVAEEDGEIVGCAALHPL-----DEDLAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFL- 91

                         90
                 ....*....|..
gi 515972316 123 tqETNVTAQKLY 134
Cdd:COG1246   92 --LTTSAAIHFY 101
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 55-122 2.51e-13

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 60.75  E-value: 2.51e-13
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 515972316  55 CAVAKQGEQVLGIVHYVIHrstWAKNNYCYLEDLYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWH 122
Cdd:cd04301    1 FLVAEDDGEIVGFASLSPD---GSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 83-134 2.13e-11

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 57.76  E-value: 2.13e-11
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 515972316  83 CYLEDLYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWHTQETNVTAQKLY 134
Cdd:COG0454   59 LELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIRFY 110
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
44-128 1.71e-10

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 55.48  E-value: 1.71e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515972316  44 ERFFDENEPMYCAVAKQGEQVLGIVHYVIHRSTWAKNnYCYLEDLYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWHT 123
Cdd:COG3153   30 DRLREDPAAGLSLVAEDDGEIVGHVALSPVDIDGEGP-ALLLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLG 108


                 ....*
gi 515972316 124 QETNV 128
Cdd:COG3153  109 DPSLL 113
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 57-134 6.55e-10

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 52.46  E-value: 6.55e-10
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 515972316   57 VAKQGEQVLGIVHYVIHRSTWaknnYCYLEDLYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWHTQEtnvTAQKLY 134
Cdd:pfam13508   7 VAEDDGKIVGFAALLPLDDEG----ALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETTN---RAAAFY 77
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
80-134 6.74e-09

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 49.91  E-value: 6.74e-09
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 515972316  80 NNYCYLEDLYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWHTQETNVTAQKLY 134
Cdd:COG3393   13 PGVAEISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYVDADNPAARRLY 67
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 15-131 3.29e-06

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 43.80  E-value: 3.29e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515972316   15 DYSQwekywLAYQNFYQVnlplhVTKVTWERFFDENEpMYCAVAKQGEQVLGIVhyvihrstwAKNNYCYLEDLYVSPEV 94
Cdd:pfam13673   4 DYSE-----EGIETFYEF-----ISPEALRERIDQGE-YFFFVAFEGGQIVGVI---------ALRDRGHISLLFVDPDY 63

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 515972316   95 RGQHIGKQLIEYVQKQAIKHNCdrlywHTQETNVTAQ 131
Cdd:pfam13673  64 QGQGIGKALLEAVEDYAEKDGI-----KLSELTVNAS 95
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
44-134 3.51e-06

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 43.63  E-value: 3.51e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515972316  44 ERFFDENEPM-YCAVAKQGEQVLGIVhyvihRSTWAKNNYCYLEDLYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWH 122
Cdd:COG2153   24 YLELDGKDEDaRHLLAYDDGELVATA-----RLLPPGDGEAKIGRVAVLPEYRGQGLGRALMEAAIEEARERGARRIVLS 98

                         90
                 ....*....|..
gi 515972316 123 TQEtnvTAQKLY 134
Cdd:COG2153   99 AQA---HAVGFY 107
PRK10975 PRK10975
dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;
88-134 1.44e-04

dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;


Pssm-ID: 182877  Cd Length: 194  Bit Score: 39.91  E-value: 1.44e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 515972316  88 LYVSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWHTQETNVTAQKLY 134
Cdd:PRK10975 132 LAVFPGAQGRGIGARLMQAALNWCQARGLTRLRVATQMGNLAALRLY 178
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 1-134 1.83e-03

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 36.90  E-value: 1.83e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 515972316   1 MQNQGLQIVPVQQQDYSQWEKYWLAYQNFYQVNLPlHVTKVTWERFFDENEP--------MYCAVAKQGEQVLGIVHYvi 72
Cdd:COG1670    3 LETERLRLRPLRPEDAEALAELLNDPEVARYLPGP-PYSLEEARAWLERLLAdwadggalPFAIEDKEDGELIGVVGL-- 79

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 515972316  73 HRSTWAkNNYCYLeDLYVSPEVRGQHIGKQLIEYVQKQAIKH-NCDRLYWHTQETNVTAQKLY 134
Cdd:COG1670   80 YDIDRA-NRSAEI-GYWLAPAYWGKGYATEALRALLDYAFEElGLHRVEAEVDPDNTASIRVL 140
PLN02825 PLN02825
amino-acid N-acetyltransferase
 90-140 6.26e-03

amino-acid N-acetyltransferase


Pssm-ID: 215441 [Multi-domain]  Cd Length: 515  Bit Score: 35.90  E-value: 6.26e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 515972316  90 VSPEVRGQHIGKQLIEYVQKQAIKHNCDRLYWHTQETNvtaqklyDWIAQK 140
Cdd:PLN02825 440 VSPECRGQGQGDKLLDYIEKKAASLGLEKLFLLTTRTA-------DWFVRR 483
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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