NCBI Conserved Domain Search

VapC-like PIN domain of Mycobacterium tuberculosis VapC4 and VapC5, and Neisseria gonorrhoeae FitB and related proteins; This family includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This family belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350229 Cd Length: 127 Bit Score: 144.93 E-value: 8.28e-46

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCICSFIMREQPEAVLKRLEQavLRRHRIVVSAITYAEMRFGCTGKKASPRHAQLVDAFCSRLDaVLAWDRAAVDAT 85
Cdd:cd09881    1 LLDTNICIDLLRGRPPSVLERLEE--LLPEDLAISSITLAELLYGAEKSQRPEKNLAALEAFLSRLE-VLPFDEEAAEIY 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 516010608  86 TEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYED 135
Cdd:cd09881   78 GEIRAELEKKGKPIGDNDLLIAATALAHGLTLVTNNTKHFERIPGLKVEN 127

Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];

Pssm-ID: 441096 Cd Length: 128 Bit Score: 134.99 E-value: 7.99e-42

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   5 YMLDTCICSFIMREQPEAVLKRLEQavLRRHRIVVSAITYAEMRFGCtGKKASPRHAQLVDAFCSRLDaVLAWDRAAVDA 84
Cdd:COG1487    1 YLLDTNVLIDLLRGRPPAVLAWLAE--LEAGELAISAITLAELLYGA-RRSPDAKRRAALEAFLEAFP-VLPFDAEAARR 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 516010608  85 TTEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYEDW 136
Cdd:COG1487   77 AGELRAALRRAGRPIGLADLLIAATALAHGLTLVTRNTKDFERIPGLKVEPW 128

VapC-like PIN domain of Haemophilus influenzae VapC1 and related proteins; Haemophilus influenzae VapC1 has endonucleolytic activity with RNA, it cleaves initiator tRNA between the anticodon stem and loop, but does not cleave mRNA, rRNA or tmRNA, and has no activity on ssDNA or dsDNA. This subfamily belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. FitB is a toxin of the FitAB TA system. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350302 Cd Length: 128 Bit Score: 133.49 E-value: 2.75e-41

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCICSFIMREQPEAVLKRLEQavLRRHRIVVSAITYAEMRFGCTGKKASPRHAQLVDAFCSRLdAVLAWDRAAVDAT 85
Cdd:cd18735    1 LLDTNICIYIRKQRPPSVLERFAQ--LQPGEVAMSVITYGELLYGAEKSQRRAKALEALERLAELI-PVLPLPEEVAEHY 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 516010608  86 TEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYEDW 136
Cdd:cd18735   78 GEIRADLEKKGTPIGNNDLWIAAHALALGLTLVTNNTREFQRVPGLKVENW 128

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350312 Cd Length: 130 Bit Score: 132.24 E-value: 1.03e-40

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   5 YMLDTCICSFIMREQPEAVLKRLEQAvlRRHRIVVSAITYAEMRFGCTgKKASPRH-AQLVDAFCSRLDaVLAWDRAAVD 83
Cdd:cd18745    1 YLLDTNTVIALFRGRGPVLRARLRAA--APSDLALSSIVLYELEYGAA-KSARPERnRARLEELLSLIE-VLPFDEEDAR 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 516010608  84 ATTEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYEDW 136
Cdd:cd18745   77 AAAEIRADLERAGTPIGPYDTLIAGQALARGATLVTHNVREFSRVPGLKVEDW 129

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350315 Cd Length: 128 Bit Score: 130.35 E-value: 5.45e-40

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCICSFIMREQPEAVLKRLEQAVLrrHRIVVSAITYAEMRFGCTgKKASPRHAQLVDAFCSRLDaVLAWDRAAVDAT 85
Cdd:cd18748    1 LLDTNIISDLIRNPRGKVARRIREVGE--DAVCTSIIVASELRFGAA-KKGSERLARRVEAVLSRIP-VLPLEPPADEHY 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 516010608  86 TEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYEDW 136
Cdd:cd18748   77 ARIRADLERQGTPIGPNDLLIAAHALALDLTLVTDNTREFSRVPGLKVENW 127

tRNA(fMet)-specific endonuclease VapC;

Pssm-ID: 184277 Cd Length: 132 Bit Score: 124.37 E-value: 1.28e-37

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   5 YMLDTCICSFIMREQPEAVLKRLEqavLRRHRIVVSAITYAEMRFGCTGKKASPRHAQLVDAFCSRLDaVLAWDRAAVDA 84
Cdd:PRK13725   4 FMLDTNICIFTIKNKPEHVRERFN---LNTGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLE-VLDYDTAAATH 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 516010608  85 TTEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYEDW 136
Cdd:PRK13725  80 TGQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVPGIRIEDW 131

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350307 Cd Length: 126 Bit Score: 114.58 E-value: 7.19e-34

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCICSFIMREQPEAVLKRLEQAVLrrHRIVVSAITYAEMRFGCTGKKASPRHAQLVDAFCSRLDaVLAWDRAAVDAT 85
Cdd:cd18740    1 MLDTNTVSHIIKGRPPAVRERLRAVPM--ASICISSITEAELLYGLAKRPEAKRLHAAVREFLLRVD-VLPWDSSVARTY 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 516010608  86 TEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNvREFERVPGLQYED 135
Cdd:cd18740   78 GDLRAALEAQGKTLGPLDMMIAAHALALGATLVTND-RAFRRVPGLAVED 126

VapC-like PIN domain of Rickettsia felis VapC2 and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Rickettsia felis VapC2, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. Rickettsia felis VapC2 cleaves single-stranded RNA. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350301 Cd Length: 129 Bit Score: 109.51 E-value: 8.19e-32

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCICSFIMREQPEAVLKRLEQaVLRRHRIVVSAITYAEMRFGCTGKKASPRHAQLVDAFCSRLDaVLAWDRAAVDAT 85
Cdd:cd18734    1 MLDTNICVYLIKKKPDSYYARLEK-LQKNNDIAISSIVLAELQYGVSKSQYKEQNQAALNMFLMKLE-VLPFEEKATFYY 78

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 516010608  86 TEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYEDW 136
Cdd:cd18734   79 GEIRAELEKSGQVIGGNDLLIAAHALAENATLVTNNIKEFARVPNLILENW 129

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350317 Cd Length: 127 Bit Score: 106.17 E-value: 1.82e-30

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCICSFIMREQPEaVLKRLeQAVLRRHrIVVSAITYAEMRFGCTGK-KASPRHAQLVDAFCSRLdAVLAWDRAAVDA 84
Cdd:cd18750    1 LLDTCTISDFVRGNPN-TLERL-KSTSPSL-IAISSITVMEIEYGLRLNpQRAKKLRPILEAFLSSI-TILPFDEEDAIA 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 516010608  85 TTEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYED 135
Cdd:cd18750   77 AAAIRAALKKQGTPIGAYDVLIAGTALAHGLILVTSNTKEFQRVDGLVIEN 127

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350311 Cd Length: 128 Bit Score: 104.88 E-value: 6.39e-30

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCICSFIMREQPeAVLKRLEQAVLRRHRIVVSAITYAEMRFGCTGKKASPRHAQLvDAFCSRLDaVLAWDRAAVDAT 85
Cdd:cd18744    1 LLDTDILSALLKGNP-KVVEKFREYLKEYGKITISIITYYEILRGLLAKNATKQLEVF-EEFCSNNE-VLPLDDESADIA 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 516010608  86 TEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYEDW 136
Cdd:cd18744   78 AEIYADLRKKGTLIDDADILIAATAIANNLVLVTNNTKHFQRIEGLKLENW 128

VapC-like PIN domain of Caulobacter Crescentus VapC1-like and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Caulobacter Crescentus VapC1, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. This subfamily belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. FitB is a toxin of the FitAB TA system. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350303 Cd Length: 123 Bit Score: 104.19 E-value: 8.41e-30

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCICSFIMREQPEAVLKRLEQavLRRHRIVVSAITYAEMRFGCtgKKASPRHAQLVDAFCSRLDaVLAWDRAAVDAT 85
Cdd:cd18736    1 LLDTNICIYLLKGQPPALARRVAA--CEPGEVVISAITLAELAYGV--RRSAPPRRAALDALLEVIP-VLPFDEAAAAAY 75

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 516010608  86 TEIRAvlaAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYEDW 136
Cdd:cd18736   76 ARIRA---AFPFRRASFDRLIAAHALALGLTLVTNNEADFADIPGLRVENW 123

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350309 Cd Length: 127 Bit Score: 94.91 E-value: 4.25e-26

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   7 LDTCICSFIMREQPEaVLKRLEQAVLRRhrIVVSAITYAEMRFGctGKKASPRHAQL--VDAFCSRLdAVLAWDRAAVDA 84
Cdd:cd18742    2 LDTDICIYWLKGNES-VKEKFLEVGNEP--LSISVITYAELYYG--AYNSQRVEKNLarVERFKELL-PVLPLSEAIAET 75

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 516010608  85 TTEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYEDW 136
Cdd:cd18742   76 FGKIKADLRKKGNPIDDFDLLIASTALANNLILVTNNTKHFKRIPGLKIENW 127

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350314 Cd Length: 132 Bit Score: 87.59 E-value: 3.52e-23

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   7 LDTCICSFIMREQP-EAVLKRLEQavlRRHRIVVSAITYAEMRFGCT----GKKASpRHAQLVDAFCSRLDaVLAWDRAA 81
Cdd:cd18747    2 LDTNILSEPLKPEPnANVLARLER---HEGEIAIAAVVWHELLYGCYrlpeGKRKR-ILEYYLEDVVSGLP-ILPYDEAA 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 516010608  82 VDATTEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYEDW 136
Cdd:cd18747   77 AEWHAEERARLTAIGKTPPFADGQIAAIAVANNLILVTRNTKDFQGFEGLRLENW 131

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350310 Cd Length: 127 Bit Score: 87.19 E-value: 5.37e-23

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   7 LDTCICSFIMREQpEAVLKRLEQAVLrrHRIVVSAITYAEMRFG--CTGKKAspRHAQLVDAFCSRLDaVL----AWDRA 80
Cdd:cd18743    2 LDTNICIFLLRGK-YGVAEKIEEVGL--ENCFISEITIAELLYGaeNSARPE--KNRKLVDDFLSLFT-VLpisdALEEY 75

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 516010608  81 AvdattEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYEDWV 137
Cdd:cd18743   76 A-----KEKARLRRQGKLIDDFDLLIGATAIANNLTLVTENVKHFARIPGIKLENWI 127

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350319 Cd Length: 130 Bit Score: 79.98 E-value: 3.54e-20

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCICSFIMREQPEAVL-KRLEQavLRRHRIVVSAITYAEMRFGCTgkkASPRHAQLVDAFCSRLDA---VLAWDRAA 81
Cdd:cd18752    1 LFDTDAISELLKKRPSPPLlERLNS--IPREDQFTSAITVGELYYGAY---RSDRRLRLLEELLEPVISrvtILPFDRES 75

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 516010608  82 VDATTEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYEDW 136
Cdd:cd18752   76 ARVYGRLRAELEKKGTPLGEADLQIAAIALAHNLTLVTGNIRHFERIPGLRVENW 130

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350316 Cd Length: 126 Bit Score: 72.27 E-value: 3.40e-17

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   7 LDTCICSFIMREQPEAvlkRLEQAVLRRHRIVVSAITYAEMRFGCTGKKASPRHAQLVDAFCSRLdAVLAWDRAAVDATT 86
Cdd:cd18749    2 LDTDVASYLFKGRLLA---ALYAPRLVGKILVISFVTVAELYFWAEKRGWGEKRLQQLEAWLSRY-VVLPYDEEIARTWG 77

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*.
gi 516010608  87 EIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQ 132
Cdd:cd18749   78 RLRAEAQRRGRPRPTNDAWIAATALTHGLPLATHNVKDFEDFPGLQ 123

VapC-like PIN domain of Neisseria gonorrhoeae FitB and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Neisseria gonorrhoeae FitB toxin of the FitAB toxin/antitoxin (TA) system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. In N. gonorrhoeae, FitA and FitB form a heterodimer: FitA is the DNA binding subunit and FitB contains a ribonuclease activity that is blocked by the presence of FitA. A tetramer of FitAB heterodimers binds DNA from the fitAB upstream promoter region with high affinity. This results in both sequestration of FitAB and repression of fitAB transcription. It is thought that FitAB release from the DNA and subsequent dissociation both slows N. gonorrhoeae replication and transcytosis by an as yet undefined mechanism. The toxin M. tuberculosis VapC is a structural homolog of N. gonorrhoeae FitB, but their antitoxin partners, VapB and FitA, respectively, differ structurally. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350298 Cd Length: 136 Bit Score: 70.91 E-value: 1.12e-16

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   7 LDTCICSFIMREQPE-AVLKRLEQavLRRHRIVVSAITYAEMRFGC----TGKKASpRHAQLVDAFCSRL--DAVLAWDR 79
Cdd:cd18731    3 LDTNVVSELMRPAPDpAVLAWLDA--QPAEDLYLTAITVAELLYGIallpDGRRRD-ALEAAAEALLAEDfaGRILPFDA 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 516010608  80 AAVDATTEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFE 126
Cdd:cd18731   80 AAAEAYAEIVAARRRAGRPISVADAQIAAIARAHGATLATRNVKDFE 126

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350318 Cd Length: 133 Bit Score: 68.15 E-value: 1.50e-15

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCICSFIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGCtgkkASPRHAQLVDAFCSRLDA--VLAWDRAAVD 83
Cdd:cd18751    1 LLDTDILSEILKGKNPQVVARARAYLAAFGRYTISVITVMEVVRGL----QKVQRAERLQQFLEMIESaeVLPLDRTAAE 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 516010608  84 ATTEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQY----EDW 136
Cdd:cd18751   77 LAGRIYADLERTGQPIGRADPMIAAIALRHGLVLVTGNTAHYQRIQELGYplvlENW 133

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350320 Cd Length: 123 Bit Score: 65.57 E-value: 1.16e-14

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCICSFIMREQPeAVLKRLEQAvlrrHRIVVSAITYAEMRFG--CTGKKAspRHAQLVDAFCSRLD-AVLAwdraaV 82
Cdd:cd18753    1 LLDTNAYSALLRGDP-AVLEALARA----DEIYLPVIVLGELLFGfrKGSREE--ENRERLEEFLSSPRvEVLP-----I 68

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 516010608  83 DATT-----EIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNvREFERVPGL 131
Cdd:cd18753   69 DEETaeiyaEIKSALKKKGTPIPENDIWIAALALEHGLPLVTRD-AHFDKIPGL 121

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350305 Cd Length: 118 Bit Score: 61.39 E-value: 4.14e-13

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   7 LDTCICSFIMREQPEAVLKRLEQAVlrrHRIVVSAITYAEMrFGCtgKKASPRHAQLVDAFCSRLDaVLAWDRAAVDATT 86
Cdd:cd18738    2 LDTNIIIYYLKGELPESLEELLEEL---NEPAISVITKIEL-LGW--PKLTPEEEEEIEDFLSLAE-VIPLDDEIARKTI 74

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 516010608  87 EIRAvlaavGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYED 135
Cdd:cd18738   75 ELRK-----QYKIKLPDAIIAATALVHGLTLVTRNVKDFKKIPGLKLLN 118

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350308 Cd Length: 120 Bit Score: 60.59 E-value: 9.56e-13

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   7 LDTCICSFIMREQPEAVlKRLEQavLRRHRIVVSAITYAEMRFGCTGKKASPRHAQLVDAFcsrldAVLAWDRAAVDATT 86
Cdd:cd18741    2 LDTDILIDFLRGNPSAA-EKLLE--LLGGNLAISVITVAELYAGARNKKELEKLEKLLSLF-----EVIPVDEEIAELAG 73

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 516010608  87 EIRAVLAAvGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYE 134
Cdd:cd18741   74 ELRRKYRK-SHGLGLPDALIAATALEHGLTLVTLNVKHFPMIEGLKLP 120

VapC-like PIN domain of Rickettsia felis VapC1 and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Rickettsia felis VapC1, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350300 Cd Length: 131 Bit Score: 56.84 E-value: 3.25e-11

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCIcsFIMREQPEAVLKRLEQAVlRRHRIVVSAITYAEMRFGC----TGKKASPRHAqLVDAFCSRLdAVLAWDRAA 81
Cdd:cd18733    3 IIDTSV--FIAAERGRLDLEALQQER-PDEDVYISAITASELLHGVhrakTPERRARREA-FVEAILEAF-PVLDFDLEV 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 516010608  82 VDATTEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYED 135
Cdd:cd18733   78 ARTHARLWAELAKRGITIGAHDLLIAATALAHGYSVLTANVRDFKRIPGLEVEE 131

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350321 Cd Length: 128 Bit Score: 54.92 E-value: 1.40e-10

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   7 LDTcicSFI---MREQPEAVLK--RLEQAvlrRHRIVVSAITYAEMRFGCTGKKASPRHAQLVDAFCSRLdAVLAWDRAA 81
Cdd:cd18754    2 LDT---SFLidlMRGDEAAIEKarELESE---SVPQRVSSPTVFELYVGVGLSAAPEEERERIESVLESK-PILPLDGES 74

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 516010608  82 VDATTEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVREFERVPGLQYED 135
Cdd:cd18754   75 ARKAGRIDGQLRREGEPIDPGDAMIAATALVHDEPVLTRNVKHFERIDGLEVES 128

PIN domain;

Pssm-ID: 426475 Cd Length: 121 Bit Score: 54.34 E-value: 2.56e-10

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608    5 YMLDTCICSFIMREQPEAvlKRLEQAVLRRHRIVVSAITYAEMRFGCTGKKASPRHAQLVDAFCSRLD-AVLAWDRAAVD 83
Cdd:pfam01850   1 IVLDTSVLIALLRGEPLH--EAARELLEAAGELVTSAIVLAELLYGLNRRLGLGKAAELVELLLLLSAlEVLPIDAELAE 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 516010608   84 ATTEIRAVLAAVGTpigsNDAAIAGHAIASGAILVTNNvREFERVPGL 131
Cdd:pfam01850  79 EAAELRLKYGKLGP----NDALIAATAKEHGAKLITFD-EDFARVAKL 121

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350313 [Multi-domain] Cd Length: 133 Bit Score: 53.98 E-value: 3.31e-10

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   7 LDTCICSFIMREQPEAVLKRLEQAVlRRHRIVVSAITYAEMRFG--CTGKKASPRHAQL-------VDAFCSRldaVLAW 77
Cdd:cd18746    2 LDTNVISELRKPRPDPGVVAWLAAV-DEDDLFLSVITIGEIRKGieRLRRRDPARAARLeawldglLLRFAGR---ILPV 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 516010608  78 DRAAVDATTEIRAVLAAvgtPIGSNDAAIAGHAIASGAILVTNNVREFER 127
Cdd:cd18746   78 DAEVARRWGRLHAPAPA---PLPVADALIAATALVHGLTLVTRNVRDFEG 124

VapC-like PIN domain of Mycobacterium tuberculosis VapC4, VapC5, and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 toxin of the VapBC toxin/antitoxin (TA) system. This family belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. M. tuberculosis VapC4 interacts with, and cleaves tRNA44Cys-GCA. M. tuberculosis VapC5 has endonucleolytic activity with RNA, this activity is low with dsRNA, and no activity has been demonstrated on dsDNA. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350335 Cd Length: 123 Bit Score: 43.94 E-value: 2.15e-06

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCIcsFIMREQpeavlKRLEQAVLRRHRIVVSAITYAEMRFGC----TGKKASPRHAQLvdAFCSRLDaVLAWDRAA 81
Cdd:cd18768    1 LADTSV--FIAQES-----GRALDGDLLPDEIAVSVVTLAELRAGVlaarDLATRARRLATL--QLAADLE-PLPIDDAV 70

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 516010608  82 VDATTEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNVrEFERVPGLQ 132
Cdd:cd18768   71 AAAWARLRLELRESGRRMRVNDSWIAATALAHDLPVVTQDA-DFDALPDLE 120

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350306 Cd Length: 124 Bit Score: 42.32 E-value: 8.07e-06

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608  36 RIVVSAITYAEMRFGcTGKKASPRHAQLVDAFCSRLDaVLAWDRAAVDATTEIRAVLAAVGTPIGSNDAAIAGHAIASGA 115
Cdd:cd18739   28 PLVTPTIALYEVYVG-AVRSAGETDLDGVREDLDWVE-PLDFDEEVAREAAQIQAELLEAGEPINPPDLMIAATARSLGA 105

                         90
                 ....*....|....*....
gi 516010608 116 ILVTNNvREFERVPGLQYE 134
Cdd:cd18739  106 TLVTRD-GDFERIDGLEVE 123

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350304 Cd Length: 115 Bit Score: 40.60 E-value: 3.16e-05

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608  23 VLKRLEQA--VLRRH-RIVVSAITYAEMRFGCTgkkasPRHAQLVDAFCSRLDaVLAWDRAAVDATTEIRAVlaavgTPI 99
Cdd:cd18737   10 YLNGVPQAraELERYeQPAISVITWMEVLVGAP-----PEEEEATRAFLSSFE-IIPLDQAIAERAVALRRQ-----SRL 78

                         90       100
                 ....*....|....*....|....*.
gi 516010608 100 GSNDAAIAGHAIASGAILVTNNVREF 125
Cdd:cd18737   79 KLPDAIILATAQVHGLILVTRNTKDF 104

VapC-like PIN domain of Mycobacterium tuberculosis VapC4, VapC5, and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 toxin of the VapBC toxin/antitoxin (TA) system. This family belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. M. tuberculosis VapC4 interacts with, and cleaves tRNA44Cys-GCA. M. tuberculosis VapC5 has endonucleolytic activity with RNA, this activity is low with dsRNA, and no activity has been demonstrated on dsDNA. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350299 Cd Length: 124 Bit Score: 39.95 E-value: 5.83e-05

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   7 LDTCIcsFIMREqpeavlkRLEQAVLRRhRIVVSAITYAEMRFGCTGKKASPRHAQLVDAFCSRLDA-VLAWDRAAVDAT 85
Cdd:cd18732    2 LDTSV--VIDLE-------RGDPADLPD-EVAISAITLAELAFGVLAADTRAERAQRLQRLLQAEDFdPLPFDAEAARAY 71

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 516010608  86 TEIRAVLAAVGTPIGS--NDAAIAGHAIASGAILVTNNVREFERVPGL 131
Cdd:cd18732   72 GRLAAAVRAAGRKPRPraNDLLIAATAIAHGLPLLTRNPDDFAGLDGL 119

VapC-like PIN domains of VapC and Smg6 ribonucleases, ribosome assembly factor NOB1, rRNA-processing protein Fcf1, Archaeoglobus fulgidus AF0591 protein, and homologs; PIN (PilT N terminus) domains of such ribonucleases as the toxins of prokaryotic toxin/antitoxin operons FitAB and VapBC, as well as, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1, are included in VapC-like this family. Also included are the PIN domains of the Pyrobaculum aerophilum Pea0151 and Archaeoglobus fulgidus AF0591 proteins and other similar archaeal homologs. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Pssm-ID: 350205 Cd Length: 129 Bit Score: 36.48 E-value: 1.15e-03

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCIcsFI---MREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGCTGKKASPRHAQLVDAFcsRLDAVLAWDRAAV 82
Cdd:cd09854    1 VLDTNV--LIallSSEPESEAAKELLALLLGDSELVIPPLVLAELLRLLARERGARRALEILELL--RALEVVEEEPALA 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 516010608  83 DATTEIRAVLAAvgTPIGSNDAAIAGHAIA-SGAILVTNNvREFERV 128
Cdd:cd09854   77 EIALEVLALGLE--RGLDFGDALILALAKElGGAVLVTND-RDFRRL 120

VapC-like PIN domain of ribonucleases (toxins), VapC and FitB, of prokaryotic toxin/antitoxin operons, Pyrococcus horikoshii protein PH0500, and other similar bacterial and archaeal homologs; PIN (PilT N terminus) domain-containing proteins of prokaryotic toxin/antitoxin (TA) operons, such as, Mycobacterium tuberculosis VapC of the VapBC (virulence associated proteins) TA operon, and Neisseria gonorrhoeae FitB of the FitAB (fast intracellular trafficking) TA operon, as well as, the archaeal Pyrococcus horikoshii protein PH0500 are included in this family. Toxins of TA operons are believed to be involved in growth inhibition by regulating translation and are nearly always co-expressed with an antitoxin, a cognate protein inhibitor, forming an inert protein complex. Disassociation of the complex activates the ribonuclease activity of the toxin. In N. gonorrhoeae, FitA and FitB form a heterodimer: FitA is the DNA binding subunit and FitB contains a ribonuclease activity that is blocked by the presence of FitA. A tetramer of FitAB heterodimers binds DNA from the fitAB upstream promoter region with high affinity. This results in both sequestration of FitAB and repression of fitAB transcription. It is thought that FitAB release from the DNA and subsequent dissociation both slows N. gonorrhoeae replication and transcytosis by an as yet undefined mechanism. The toxin M. tuberculosis VapC is a structural homolog of N. gonorrhoeae FitB, but their antitoxin partners, VapB and FitA, respectively, differ structurally. The M. tuberculosis VapC-5 is proposed to be both an endoribonuclease and an exoribonuclease that can act on free RNA in a similar manner to the endo and exonuclease Flap endonuclease-1 (FEN1). VapC-like toxins are structural homologs of FEN1-like PIN domains, but lack the extensive arch/clamp region and the H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region, seen in FEN1-like PIN domains. PIN domains within this group typically contain three or four conserved acidic residues that cluster at the C-terminal end of the beta-sheet and form a negatively charged pocket near the center of the molecule. These putative active site residues are thought to bind Mg2+ and/or Mn2+ ions and be essential for single-stranded ribonuclease activity. VapC-like PIN domains are single domain proteins that form dimers and dimerization configures the active sites in a groove along the long-axis of the structure.

Pssm-ID: 350223 Cd Length: 130 Bit Score: 35.62 E-value: 2.26e-03

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516010608   6 MLDTCICSFIMREQPEAVLKRLEQAvLRRHRIVVSAITYAEMRFGCTGKKASP------RHAQLVDAFCSRLDavLAWDR 79
Cdd:cd09875    1 LLDTSVLIWLLDNDPKSHLRSLADR-IERGKVYVSIVSRWELAIGVNINKLRLnyafadLENLLADLAIPVLP--LTPRI 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 516010608  80 AAVDAttEIRAVLAAVGTPIGSNDAAIAGHAIASGAILVTNNvREFERVPGLQYE 134
Cdd:cd09875   78 ADEYL--EACRNLPLRGRHRSPFDRMIAAQALQHGFTVAHRD-EDFKAINAVNLE 129