NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|516234447|ref|WP_017638410|]
View 

MULTISPECIES: GNAT family N-acetyltransferase [Staphylococcus]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10456837)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  15581578|10940244|9175471|27367672|26818562
SCOP:  3000403

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
PhnO super family cl42766
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 39-147 4.07e-10

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


The actual alignment was detected with superfamily member COG0454:

Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 54.29  E-value: 4.07e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516234447  39 LRIEMINARLRHCQDMI-----LIIEDNQELQALIWGHFEKQtKVVTIELLFVYPKYRRQGFARQLKLALEDWAMKEGAQ 113
Cdd:COG0454   15 LLIEALDAELKAMEGSLagaefIAVDDKGEPIGFAGLRRLDD-KVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCT 93

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|.
gi 516234447 114 TIQSTINTKNQAMLNLNHQIGYEDTH-------IKMKKDLK 147
Cdd:COG0454   94 ALELDTLDGNPAAIRFYERLGFKEIEryvayvgGEFEKELS 134
 
Name Accession Description Interval E-value
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 39-147 4.07e-10

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 54.29  E-value: 4.07e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516234447  39 LRIEMINARLRHCQDMI-----LIIEDNQELQALIWGHFEKQtKVVTIELLFVYPKYRRQGFARQLKLALEDWAMKEGAQ 113
Cdd:COG0454   15 LLIEALDAELKAMEGSLagaefIAVDDKGEPIGFAGLRRLDD-KVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCT 93

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|.
gi 516234447 114 TIQSTINTKNQAMLNLNHQIGYEDTH-------IKMKKDLK 147
Cdd:COG0454   94 ALELDTLDGNPAAIRFYERLGFKEIEryvayvgGEFEKELS 134
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 42-135 5.68e-10

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 53.29  E-value: 5.68e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516234447   42 EMINARLRHCQDMILIIEDNQELQALIWGHFE-KQTKVVTIELLFVYPKYRRQGFARQLKLALEDWAMKEGAQTIQSTIN 120
Cdd:pfam00583  22 DLLEDWDEDASEGFFVAEEDGELVGFASLSIIdDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVA 101

                          90
                  ....*....|....*
gi 516234447  121 TKNQAMLNLNHQIGY 135
Cdd:pfam00583 102 ADNLAAIALYEKLGF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 55-115 1.12e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 48.81  E-value: 1.12e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 516234447  55 ILIIEDNQELQALIWGHFEKQT-KVVTIELLFVYPKYRRQGFARQLKLALEDWAMKEGAQTI 115
Cdd:cd04301    1 FLVAEDDGEIVGFASLSPDGSGgDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRL 62
PRK03624 PRK03624
putative acetyltransferase; Provisional
 84-137 3.24e-05

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 41.07  E-value: 3.24e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 516234447  84 LFVYPKYRRQGFARQLKLALEDWAMKEGAQTIQSTINTKNQAMLNLNHQIGYED 137
Cdd:PRK03624  74 LAVHPDFRGRGIGRALVARLEKKLIARGCPKINLQVREDNDAVLGFYEALGYEE 127
 
Name Accession Description Interval E-value
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 39-147 4.07e-10

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 54.29  E-value: 4.07e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516234447  39 LRIEMINARLRHCQDMI-----LIIEDNQELQALIWGHFEKQtKVVTIELLFVYPKYRRQGFARQLKLALEDWAMKEGAQ 113
Cdd:COG0454   15 LLIEALDAELKAMEGSLagaefIAVDDKGEPIGFAGLRRLDD-KVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCT 93

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|.
gi 516234447 114 TIQSTINTKNQAMLNLNHQIGYEDTH-------IKMKKDLK 147
Cdd:COG0454   94 ALELDTLDGNPAAIRFYERLGFKEIEryvayvgGEFEKELS 134
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
14-139 5.03e-10

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 54.62  E-value: 5.03e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516234447  14 EVATIHEKLLESQEANYKATSTSIALRIEMINARLRHCQdMILIIEDNQELQALIWGH----FEKQTKVVtIELLFVYPK 89
Cdd:COG1247   14 AIAAIYNEAIAEGTATFETEPPSEEEREAWFAAILAPGR-PVLVAEEDGEVVGFASLGpfrpRPAYRGTA-EESIYVDPD 91

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 516234447  90 YRRQGFARQLKLALEDWAMKEGAQTIQSTINTKNQAMLNLNHQIGYEDTH 139
Cdd:COG1247   92 ARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYEKLGFEEVG 141
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 68-147 5.28e-10

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 52.74  E-value: 5.28e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516234447  68 IWGHFEKQTKVVTIELLFVYPKYRRQGFARQLKLALEDWAMKEGAQTIQSTINTKNQAMLNLNHQIGYE----------D 137
Cdd:COG0456    3 ALLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEevgerpnyygD 82

                         90
                 ....*....|
gi 516234447 138 THIKMKKDLK 147
Cdd:COG0456   83 DALVMEKELA 92
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 42-135 5.68e-10

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 53.29  E-value: 5.68e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516234447   42 EMINARLRHCQDMILIIEDNQELQALIWGHFE-KQTKVVTIELLFVYPKYRRQGFARQLKLALEDWAMKEGAQTIQSTIN 120
Cdd:pfam00583  22 DLLEDWDEDASEGFFVAEEDGELVGFASLSIIdDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARERGCERIFLEVA 101

                          90
                  ....*....|....*
gi 516234447  121 TKNQAMLNLNHQIGY 135
Cdd:pfam00583 102 ADNLAAIALYEKLGF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 55-115 1.12e-08

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 48.81  E-value: 1.12e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 516234447  55 ILIIEDNQELQALIWGHFEKQT-KVVTIELLFVYPKYRRQGFARQLKLALEDWAMKEGAQTI 115
Cdd:cd04301    1 FLVAEDDGEIVGFASLSPDGSGgDTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRL 62
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
78-138 2.59e-07

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 45.67  E-value: 2.59e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 516234447  78 VVTIELLFVYPKYRRQGFARQLKLALEDWAMKEGAQTIQSTINTKNQAMLNLNHQIGYEDT 138
Cdd:COG3393   15 VAEISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYVDADNPAARRLYERLGFRPV 75
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 81-146 9.49e-07

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 44.95  E-value: 9.49e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 516234447   81 IELLFVYPKYRRQGFARQLKLALEDWAMKEGAQTIQSTINTKNQAmLNLNHQIGYEDT----------HIKMKKDL 146
Cdd:pfam13673  54 ISLLFVDPDYQGQGIGKALLEAVEDYAEKDGIKLSELTVNASPYA-VPFYEKLGFRATgpeqefngirFVPMEKEL 128
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
46-115 1.48e-06

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 44.69  E-value: 1.48e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 516234447  46 ARLRH--CQDMILIIEDNQELQ---ALIWGHFEKQTKVVTIELLFVYPKYRRQGFARQLKLALEDWAMKEGAQTI 115
Cdd:COG3153   30 DRLREdpAAGLSLVAEDDGEIVghvALSPVDIDGEGPALLLGPLAVDPEYRGQGIGRALMRAALEAARERGARAV 104
PRK03624 PRK03624
putative acetyltransferase; Provisional
 84-137 3.24e-05

putative acetyltransferase; Provisional


Pssm-ID: 235142 [Multi-domain]  Cd Length: 140  Bit Score: 41.07  E-value: 3.24e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 516234447  84 LFVYPKYRRQGFARQLKLALEDWAMKEGAQTIQSTINTKNQAMLNLNHQIGYED 137
Cdd:PRK03624  74 LAVHPDFRGRGIGRALVARLEKKLIARGCPKINLQVREDNDAVLGFYEALGYEE 127
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 85-136 1.42e-04

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 39.98  E-value: 1.42e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 516234447  85 FVYPKYRRQGFARQLKLALEDWAMKE-GAQTIQSTINTKNQAMLNLNHQIGYE 136
Cdd:COG1670   94 WLAPAYWGKGYATEALRALLDYAFEElGLHRVEAEVDPDNTASIRVLEKLGFR 146
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 55-125 1.70e-04

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 38.20  E-value: 1.70e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 516234447   55 ILIIEDNQELQALIWGHFEKQTKVVTIELLFVYPKYRRQGFARQLkLALEDWAMKEGAQTIQSTINTKNQA 125
Cdd:pfam13508   5 FFVAEDDGKIVGFAALLPLDDEGALAELRLAVHPEYRGQGIGRAL-LEAAEAAAKEGGIKLLELETTNRAA 74
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 77-115 2.20e-04

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 38.82  E-value: 2.20e-04
                         10        20        30
                 ....*....|....*....|....*....|....*....
gi 516234447  77 KVVTIELLFVYPKYRRQGFARQLKLALEDWAMKEGAQTI 115
Cdd:COG1246   51 DLAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRL 89
PRK10975 PRK10975
dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;
80-134 6.67e-04

dTDP-4-amino-4,6-dideoxy-D-galactose acyltransferase;


Pssm-ID: 182877  Cd Length: 194  Bit Score: 37.99  E-value: 6.67e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 516234447  80 TIELLFVYPKYRRQGFARQLKLALEDWAMKEGAQTIQSTINTKNQAMLNLNHQIG 134
Cdd:PRK10975 128 RIGLLAVFPGAQGRGIGARLMQAALNWCQARGLTRLRVATQMGNLAALRLYIRSG 182
COG3375 COG3375
Predicted acetyltransferase, GNAT superfamily [General function prediction only];
86-130 2.73e-03

Predicted acetyltransferase, GNAT superfamily [General function prediction only];


Pssm-ID: 442602 [Multi-domain]  Cd Length: 271  Bit Score: 36.46  E-value: 2.73e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 516234447  86 VYPKYRRQGFARQLKLALEDWAMKEGAQTIQSTI---NTKNqAMLNLN 130
Cdd:COG3375   83 VLPEYRGRGVGYALKLHQREWALERGVTLITWTFdplQARN-AYFNLA 129
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
77-146 2.78e-03

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 35.55  E-value: 2.78e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516234447  77 KVVTIELLFVYPKYRRQGFARQLKLALEDWAMKEGAQTIQstintknqamlnLNHQI---------GYEDT--------- 138
Cdd:COG2153   57 GEAKIGRVAVLPEYRGQGLGRALMEAAIEEARERGARRIV------------LSAQAhavgfyeklGFVPVgeefleagi 124


                 ....*....
gi 516234447 139 -HIKMKKDL 146
Cdd:COG2153  125 pHIDMRKPL 133
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH