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Conserved domains on  [gi|516970009|ref|WP_018190447|]
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family 43 glycosylhydrolase [Leifsonia sp. 109]

Protein Classification

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
GH43_ABN-like cd18616
Glycosyl hydrolase family 43 such as arabinan endo-1 5-alpha-L-arabinosidase; This glycosyl ...
49-360 3.11e-129

Glycosyl hydrolase family 43 such as arabinan endo-1 5-alpha-L-arabinosidase; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes with endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activity. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


:

Pssm-ID: 350128 [Multi-domain]  Cd Length: 291  Bit Score: 383.85  E-value: 3.11e-129
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  49 GGKLAEQCADPDVIRSEDPsdpVWYLYCTRDVIDSSLRnpdgslvFSSIPTYRSADLVHWSFVGEALPTKPAWIG--DGD 126
Cdd:cd18616    1 NPVFEPTFADPTVIRGDDG---YFYAYATEDPWGDGGG-------FRLVPILRSKDLVNWEYVGDAFTSKPRWKWdpGGG 70
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 127 MWAPDVAYVGGRYLLYYTATNTVApGGGSAIGVATSASPAGPWVDSGGPVvepmpspgSADPADRRWVFDPELVTAGGEH 206
Cdd:cd18616   71 LWAPDIRYIDGKYVLYYSLSDWGA-DPNPGIGVATADSPAGPFTDQGKLF--------DSNEIGVRNSIDPFVFEDDGKK 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 207 YLYFGSYYGgLSVRRLSADGLHSDPASQVQVAiGNRYEGTHVIEHDGWFYLLGSATNCCSGPLTGYGVFAGRSTSPTGPF 286
Cdd:cd18616  142 YLFWGSFYG-IYAVELTADGLALKPGEKVQIA-GDRYEGPYIVKRDGYYYLFGSAGSCCEGPNSTYRVVVGRSESLLGPY 219
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 516970009 287 EDRTGALLLDPRVGGTPVLHQNGNRWIGTGHVTSAVDATGQRWMLYHAVDRTDPYVAGGgtYTKRPVLMDALDW 360
Cdd:cd18616  220 VDRDGRSLLDSGGGGTPVVLQNGNRFVGPGHNAVITDDAGQDWMLYHAYDRNDPYLPGG--YNRRPLLLDRLDW 291
GH43_C2 super family cl38978
Beta xylosidase C-terminal Concanavalin A-like domain; This domain is found to the C-terminus ...
409-597 9.04e-03

Beta xylosidase C-terminal Concanavalin A-like domain; This domain is found to the C-terminus of the pfam04616 domain. This domain adopts a concanavalin A-like fold.


The actual alignment was detected with superfamily member pfam17851:

Pssm-ID: 436093  Cd Length: 203  Bit Score: 38.02  E-value: 9.04e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  409 SDEFDGAEMDPAWTWERDPGAATR-SLS--GGALHWDTQAAdlGPDPGAAGLPLRPLPSGDYVVETRVSSTVPSEGCCqn 485
Cdd:pfam17851   1 RDDFDSPKLGLQWQWLRNPRDESWySLTerPGYLRLYGRES--LSSLFAPSLLARRQQHFSFTATTKLEFEPQKEGEE-- 76
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  486 yaqGGLVIHQDDANFVKLSVTSIWETRQTEFGKRETAEGPGYPTYGNAVG-GPVAEATVLRLsrthTATENVYTASTSVd 564
Cdd:pfam17851  77 ---AGLVVYYNEYNHYYLGVTKDEDGGRVLRLVRCDNGELTEELAEEEVPlGGEVKTVYLRV----EVDGDTYQFSYSY- 148
                         170       180       190
                  ....*....|....*....|....*....|...
gi 516970009  565 DGATWDEAGAWttpLQATPprLGLVSMAAGGFT 597
Cdd:pfam17851 149 DGKDWKTIGPE---LDASI--LSDEYAAGGGFT 176
 
Name Accession Description Interval E-value
GH43_ABN-like cd18616
Glycosyl hydrolase family 43 such as arabinan endo-1 5-alpha-L-arabinosidase; This glycosyl ...
49-360 3.11e-129

Glycosyl hydrolase family 43 such as arabinan endo-1 5-alpha-L-arabinosidase; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes with endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activity. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350128 [Multi-domain]  Cd Length: 291  Bit Score: 383.85  E-value: 3.11e-129
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  49 GGKLAEQCADPDVIRSEDPsdpVWYLYCTRDVIDSSLRnpdgslvFSSIPTYRSADLVHWSFVGEALPTKPAWIG--DGD 126
Cdd:cd18616    1 NPVFEPTFADPTVIRGDDG---YFYAYATEDPWGDGGG-------FRLVPILRSKDLVNWEYVGDAFTSKPRWKWdpGGG 70
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 127 MWAPDVAYVGGRYLLYYTATNTVApGGGSAIGVATSASPAGPWVDSGGPVvepmpspgSADPADRRWVFDPELVTAGGEH 206
Cdd:cd18616   71 LWAPDIRYIDGKYVLYYSLSDWGA-DPNPGIGVATADSPAGPFTDQGKLF--------DSNEIGVRNSIDPFVFEDDGKK 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 207 YLYFGSYYGgLSVRRLSADGLHSDPASQVQVAiGNRYEGTHVIEHDGWFYLLGSATNCCSGPLTGYGVFAGRSTSPTGPF 286
Cdd:cd18616  142 YLFWGSFYG-IYAVELTADGLALKPGEKVQIA-GDRYEGPYIVKRDGYYYLFGSAGSCCEGPNSTYRVVVGRSESLLGPY 219
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 516970009 287 EDRTGALLLDPRVGGTPVLHQNGNRWIGTGHVTSAVDATGQRWMLYHAVDRTDPYVAGGgtYTKRPVLMDALDW 360
Cdd:cd18616  220 VDRDGRSLLDSGGGGTPVVLQNGNRFVGPGHNAVITDDAGQDWMLYHAYDRNDPYLPGG--YNRRPLLLDRLDW 291
XynB2 COG3507
Beta-xylosidase [Carbohydrate transport and metabolism];
37-382 6.21e-68

Beta-xylosidase [Carbohydrate transport and metabolism];


Pssm-ID: 442730 [Multi-domain]  Cd Length: 351  Bit Score: 226.37  E-value: 6.21e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  37 VTYSNPLpldlgggkLAEQCADPDVIRSEDpsdpVWYLYCTRDVidsslrnpdgslVFSSIPTYRSADLVHWSFVGEALP 116
Cdd:COG3507   20 NTYTNPV--------LPGDYPDPSIIRVGD----TYYLYGTSFE------------YFPGLPIFHSKDLVNWELVGHALD 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 117 TKPAWI--GDGDMWAPDVAYVGGRYLLYYTATNTVAPGGGsaIGVATSASPAGPWVDSgGPVVEPMPspgsadpadrrWV 194
Cdd:COG3507   76 RLPQWAdpYSGGIWAPDIRYHNGKYYLYYTAVDGGKNRSG--IGVATADDPEGPWSDP-GPLVCPGG-----------NG 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 195 FDPEL-VTAGGEHYLYFGSYYGGLSVRRLSADGLHSDPASQV--QVAIGNRYEGTHVIEHDGWFYLLGSATNCCSgplTG 271
Cdd:COG3507  142 IDPSVfVDDDGKAYLVYGSGGGGIYVAELDPDTGKLLGEPKTlaPGGEGGWIEGPHIYKRNGYYYLFYSEGGTCN---SG 218
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 272 YGVFAGRSTSPTGPFEDRtgallldprvGGTPVLHQN-GNRWIGTGHVTSAVDATGQRWMLYHAVDRTDPYvagggtytK 350
Cdd:COG3507  219 YAVRVARSKSPTGPYEDA----------PGNPILTQRsDGGIQGPGHGSLVETPDGEWYLVYHAYRPPGGL--------G 280
                        330       340       350
                 ....*....|....*....|....*....|...
gi 516970009 351 RPVLMDALDW-QDGWPVVRGGAGPSDSPQPAPV 382
Cdd:COG3507  281 RETFLDPVTWnEDGWPVVGPGTGEPPQPLPAPE 313
Glyco_hydro_43 pfam04616
Glycosyl hydrolases family 43; The glycosyl hydrolase family 43 contains members that are ...
57-365 4.63e-41

Glycosyl hydrolases family 43; The glycosyl hydrolase family 43 contains members that are arabinanases. Arabinanases hydrolyse the alpha-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. The structure of arabinanase Arb43A from Cellvibrio japonicus reveals a five-bladed beta-propeller fold. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 398349 [Multi-domain]  Cd Length: 281  Bit Score: 151.32  E-value: 4.63e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009   57 ADPDVIRSEDPsdpvWYLYCTrdvidsslrnpdGSLVFSSIPTYRSADLVHWSFVGEALPTKPAWIGDGDM--WAPDVAY 134
Cdd:pfam04616  11 PDPSILRVGDD----YYLTTS------------SFEWFPGIPIFHSKDLVNWKLVGPVLVRRSQLSGRGSNasWAPDISY 74
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  135 VGGRYLLYYTATNtvapgggSAIGVATSASPAGPWVDSGgpvvePMPSPGSAdpadrrwvFDPELVTAG-GEHYLYFGSY 213
Cdd:pfam04616  75 HDGKYYLYYTAVA-------HGIFVATADSPDGPWSDPG-----KLKSGGGG--------IDPSLFHDDdGKKYLVWGGW 134
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  214 -----YGGLSVRRLSADGLHSDPASQVQVAIGNRY------EGTHVIEHDGWFYLLGSATNCCSGPLTGYgvfaGRSTSP 282
Cdd:pfam04616 135 dprhgHGGIYLQELDNDGLKLVGPVTKLIYPGTRWvggkvtEGPHLYKRNGYYYLTYAAGGTGGPYAVGV----ARSRSP 210
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  283 TGPFEDRTgallldprvgGTPVLHQNG--NRWIGTGHVTSAVDATGQRWMLYHAVDrtdpyVAGGGTYTKRPVLMDALDW 360
Cdd:pfam04616 211 LGPYEWHP----------GNPILTSRSpeNPIYGPGHASLVETPDGEWWIVYHAGR-----PGDGGYGLGRETRIQPVEW 275

                  ....*.
gi 516970009  361 -QDGWP 365
Cdd:pfam04616 276 rADGWP 281
GH43_C2 pfam17851
Beta xylosidase C-terminal Concanavalin A-like domain; This domain is found to the C-terminus ...
409-597 9.04e-03

Beta xylosidase C-terminal Concanavalin A-like domain; This domain is found to the C-terminus of the pfam04616 domain. This domain adopts a concanavalin A-like fold.


Pssm-ID: 436093  Cd Length: 203  Bit Score: 38.02  E-value: 9.04e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  409 SDEFDGAEMDPAWTWERDPGAATR-SLS--GGALHWDTQAAdlGPDPGAAGLPLRPLPSGDYVVETRVSSTVPSEGCCqn 485
Cdd:pfam17851   1 RDDFDSPKLGLQWQWLRNPRDESWySLTerPGYLRLYGRES--LSSLFAPSLLARRQQHFSFTATTKLEFEPQKEGEE-- 76
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  486 yaqGGLVIHQDDANFVKLSVTSIWETRQTEFGKRETAEGPGYPTYGNAVG-GPVAEATVLRLsrthTATENVYTASTSVd 564
Cdd:pfam17851  77 ---AGLVVYYNEYNHYYLGVTKDEDGGRVLRLVRCDNGELTEELAEEEVPlGGEVKTVYLRV----EVDGDTYQFSYSY- 148
                         170       180       190
                  ....*....|....*....|....*....|...
gi 516970009  565 DGATWDEAGAWttpLQATPprLGLVSMAAGGFT 597
Cdd:pfam17851 149 DGKDWKTIGPE---LDASI--LSDEYAAGGGFT 176
 
Name Accession Description Interval E-value
GH43_ABN-like cd18616
Glycosyl hydrolase family 43 such as arabinan endo-1 5-alpha-L-arabinosidase; This glycosyl ...
49-360 3.11e-129

Glycosyl hydrolase family 43 such as arabinan endo-1 5-alpha-L-arabinosidase; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes with endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activity. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350128 [Multi-domain]  Cd Length: 291  Bit Score: 383.85  E-value: 3.11e-129
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  49 GGKLAEQCADPDVIRSEDPsdpVWYLYCTRDVIDSSLRnpdgslvFSSIPTYRSADLVHWSFVGEALPTKPAWIG--DGD 126
Cdd:cd18616    1 NPVFEPTFADPTVIRGDDG---YFYAYATEDPWGDGGG-------FRLVPILRSKDLVNWEYVGDAFTSKPRWKWdpGGG 70
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 127 MWAPDVAYVGGRYLLYYTATNTVApGGGSAIGVATSASPAGPWVDSGGPVvepmpspgSADPADRRWVFDPELVTAGGEH 206
Cdd:cd18616   71 LWAPDIRYIDGKYVLYYSLSDWGA-DPNPGIGVATADSPAGPFTDQGKLF--------DSNEIGVRNSIDPFVFEDDGKK 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 207 YLYFGSYYGgLSVRRLSADGLHSDPASQVQVAiGNRYEGTHVIEHDGWFYLLGSATNCCSGPLTGYGVFAGRSTSPTGPF 286
Cdd:cd18616  142 YLFWGSFYG-IYAVELTADGLALKPGEKVQIA-GDRYEGPYIVKRDGYYYLFGSAGSCCEGPNSTYRVVVGRSESLLGPY 219
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 516970009 287 EDRTGALLLDPRVGGTPVLHQNGNRWIGTGHVTSAVDATGQRWMLYHAVDRTDPYVAGGgtYTKRPVLMDALDW 360
Cdd:cd18616  220 VDRDGRSLLDSGGGGTPVVLQNGNRFVGPGHNAVITDDAGQDWMLYHAYDRNDPYLPGG--YNRRPLLLDRLDW 291
XynB2 COG3507
Beta-xylosidase [Carbohydrate transport and metabolism];
37-382 6.21e-68

Beta-xylosidase [Carbohydrate transport and metabolism];


Pssm-ID: 442730 [Multi-domain]  Cd Length: 351  Bit Score: 226.37  E-value: 6.21e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  37 VTYSNPLpldlgggkLAEQCADPDVIRSEDpsdpVWYLYCTRDVidsslrnpdgslVFSSIPTYRSADLVHWSFVGEALP 116
Cdd:COG3507   20 NTYTNPV--------LPGDYPDPSIIRVGD----TYYLYGTSFE------------YFPGLPIFHSKDLVNWELVGHALD 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 117 TKPAWI--GDGDMWAPDVAYVGGRYLLYYTATNTVAPGGGsaIGVATSASPAGPWVDSgGPVVEPMPspgsadpadrrWV 194
Cdd:COG3507   76 RLPQWAdpYSGGIWAPDIRYHNGKYYLYYTAVDGGKNRSG--IGVATADDPEGPWSDP-GPLVCPGG-----------NG 141
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 195 FDPEL-VTAGGEHYLYFGSYYGGLSVRRLSADGLHSDPASQV--QVAIGNRYEGTHVIEHDGWFYLLGSATNCCSgplTG 271
Cdd:COG3507  142 IDPSVfVDDDGKAYLVYGSGGGGIYVAELDPDTGKLLGEPKTlaPGGEGGWIEGPHIYKRNGYYYLFYSEGGTCN---SG 218
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 272 YGVFAGRSTSPTGPFEDRtgallldprvGGTPVLHQN-GNRWIGTGHVTSAVDATGQRWMLYHAVDRTDPYvagggtytK 350
Cdd:COG3507  219 YAVRVARSKSPTGPYEDA----------PGNPILTQRsDGGIQGPGHGSLVETPDGEWYLVYHAYRPPGGL--------G 280
                        330       340       350
                 ....*....|....*....|....*....|...
gi 516970009 351 RPVLMDALDW-QDGWPVVRGGAGPSDSPQPAPV 382
Cdd:COG3507  281 RETFLDPVTWnEDGWPVVGPGTGEPPQPLPAPE 313
GH43_Arb43a-like cd08998
Glycosyl hydrolase family 43 protein such as Bacillus subtilis subsp. subtilis str. 168 ...
58-343 1.12e-56

Glycosyl hydrolase family 43 protein such as Bacillus subtilis subsp. subtilis str. 168 endo-alpha-1,5-L-arabinanase Arb43A; This glycosyl hydrolase family 43 (GH43) subgroup belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. Many of these enzymes such as the Bacillus subtilis arabinanase Abn2, that hydrolyzes sugar beet arabinan (branched), linear alpha-1,5-L-arabinan and pectin, are different from other arabinases; they are organized into two different domains with a divalent metal cluster close to the catalytic residues to guarantee the correct protonation state of the catalytic residues and consequently the enzyme activity. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350112 [Multi-domain]  Cd Length: 278  Bit Score: 193.92  E-value: 1.12e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  58 DPDVIRseDPSDpVWYLYCTRDvidsslrnpdgslvfsSIPTYRSADLVHWSFVGEALPTKPAWI------GDGDMWAPD 131
Cdd:cd08998    3 DPSIIK--DDGG-TYYVFSTGA----------------GIQIRTSKDLVNWEFVGTVFPEGPAWAaaevpgGAGGLWAPD 63
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 132 VAYVGGRYLLYYTA----TNTvapgggSAIGVATSASPA-GPWVDsGGPVVEpmpSPGSA-----DPAdrrwVFdpelVT 201
Cdd:cd08998   64 VVYVNGRYYLYYSAstfgSNR------SAIGLATSTTLDdGPWTD-QGLVVS---SSPGDdynaiDPN----VF----VD 125
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 202 AGGEHYLYFGSYYGGLSVRRLSADGLHSDPASQVQ-VAIGNRY----EGTHVIEHDGWFYLLGSATNCCSGPLTGYGVFA 276
Cdd:cd08998  126 ADGRLWLAYGSFWGGIKLVELDPATGKLRPGSTGTsIASRPGGpgaiEAPYIIYRGGYYYLFVSYGSCCRGANSTYNIRV 205
                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 516970009 277 GRSTSPTGPFEDRTGALLLDPrvGGTPVLhQNGNRWIGTGHVtSAVDATGQRWMLYHAVDRTDPYVA 343
Cdd:cd08998  206 GRSTSITGPYVDRNGVDMLEG--GGTLLL-GGHGRWIGPGHN-SVFRDGDGDYLVYHYYDGDDGGAP 268
GH43_ABN-like cd08999
Glycosyl hydrolase family 43 protein such as endo-alpha-L-arabinanase; This glycosyl hydrolase ...
52-366 3.62e-53

Glycosyl hydrolase family 43 protein such as endo-alpha-L-arabinanase; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes with alpha-L-arabinofuranosidase (ABF; EC 3.2.1.55) and endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350113 [Multi-domain]  Cd Length: 284  Bit Score: 184.65  E-value: 3.62e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  52 LAEQCADPDVIRSEDpsdpVWYLYCTRdvidSSLRNpdgslvfssIPTYRSADLVHWSFVG-EALPTKPAWIG-DGDMWA 129
Cdd:cd08999    4 IDGDFPDPSVIRVGG----TYYAFATN----SGGKN---------VQVATSTDLVTWTLLGgDALPDLPAWAAaGGNTWA 66
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 130 PDVAYV-GGRYLLYYTATNtvAPGGGSAIGVATSASPAGPWVDSGGPVVEPMPSPGSADPAdrrwVFdpelVTAGGEHYL 208
Cdd:cd08999   67 PDVVRRpDGKYVMYYSARL--KSSGKHCIGVATSDSPLGPFTPVGEPPLCPLDQGGAIDPS----GF----VDPDGKRYL 136
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 209 YFGSYygGLS--------VRRLSADGLH-SDPASQVQVAIGNRY----EGTHVIEHDGWFYLLGSAtNCCSGPltGYGVF 275
Cdd:cd08999  137 VYKVD--GNSigvptpimLQELSADGLTlVGEPVELLLNDGPWDgplvEAPSLVKRDGTYYLFYSS-NCYCSP--SYAVG 211
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 276 AGRSTSPTGPFEDRtgallldprvgGTPVLHQNGNRWIGTGHVTSAVDAtGQRWMLYHAvdrtdpYVAGGGTYTKRPVLM 355
Cdd:cd08999  212 YATSKSITGPYTKA-----------GEPLLLTGDGGLTGPGGADVVEDD-GGDWMVFHA------WDGGDDVGGGRAMYT 273
                        330
                 ....*....|.
gi 516970009 356 DALDWQDGWPV 366
Cdd:cd08999  274 AELTWEGGWPV 284
GH43_ABN cd08988
Glycosyl hydrolase family 43; This glycosyl hydrolase family 43 (GH43) subgroup includes ...
57-360 1.18e-44

Glycosyl hydrolase family 43; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes with alpha-L-arabinofuranosidase (ABF; EC 3.2.1.55) and endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350102 [Multi-domain]  Cd Length: 277  Bit Score: 161.14  E-value: 1.18e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  57 ADPDVIRseDPSdpVWYLYCTrdvidsslrNPDGSLvfssIPTYRSADLVHWSFVGEALPTKPAWIG------DGDMWAP 130
Cdd:cd08988    1 HDPSIIK--EGG--TYYAFGT---------GTDGFG----IPIAKSKDLGNWTIVGEAFATLPSWKGgsppsaDGNLWAP 63
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 131 DVAYVGGRYLLYYTATNTVAPGggSAIGVATSASPAGPWVDSGGPV--VEPMPSPGSADPAdrrWVFDPElvtagGEHYL 208
Cdd:cd08988   64 DISQHKGKYYLYYSVSDNGSNT--SAIGLATANNPQGPFKDEGPAKpvVTSDNAGNAIDPD---LFQDED-----GQNWL 133
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 209 YFGSYYGGLSVRRLSADGLHSDPAS---QVQVAIGNRYEGTHVIEHDGWFYLLGSATNCCSGPLTGYGVFAGRSTSPTGP 285
Cdd:cd08988  134 LYGSFWGGIWLQKLDKNGLVVNPPGngkSIAVLYYVSIEAPYITYAGGYYYLFVSAGSCCDGGNSTYHTRVGRSKKVTGP 213
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 516970009 286 FEDRTGallLDPRVGGTPVLHQNGNRWIGTGHVTSAVDATGQRWMLYHAVDRtdpyvaggGTYTKRPVLMDALDW 360
Cdd:cd08988  214 YLDKGG---LDMLEGGGTLLTKGKNQWVGPGHNSIVTGDNGVDYLVLHAYDA--------NDNSSRKLYILSLFW 277
Glyco_hydro_43 pfam04616
Glycosyl hydrolases family 43; The glycosyl hydrolase family 43 contains members that are ...
57-365 4.63e-41

Glycosyl hydrolases family 43; The glycosyl hydrolase family 43 contains members that are arabinanases. Arabinanases hydrolyse the alpha-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. The structure of arabinanase Arb43A from Cellvibrio japonicus reveals a five-bladed beta-propeller fold. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 398349 [Multi-domain]  Cd Length: 281  Bit Score: 151.32  E-value: 4.63e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009   57 ADPDVIRSEDPsdpvWYLYCTrdvidsslrnpdGSLVFSSIPTYRSADLVHWSFVGEALPTKPAWIGDGDM--WAPDVAY 134
Cdd:pfam04616  11 PDPSILRVGDD----YYLTTS------------SFEWFPGIPIFHSKDLVNWKLVGPVLVRRSQLSGRGSNasWAPDISY 74
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  135 VGGRYLLYYTATNtvapgggSAIGVATSASPAGPWVDSGgpvvePMPSPGSAdpadrrwvFDPELVTAG-GEHYLYFGSY 213
Cdd:pfam04616  75 HDGKYYLYYTAVA-------HGIFVATADSPDGPWSDPG-----KLKSGGGG--------IDPSLFHDDdGKKYLVWGGW 134
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  214 -----YGGLSVRRLSADGLHSDPASQVQVAIGNRY------EGTHVIEHDGWFYLLGSATNCCSGPLTGYgvfaGRSTSP 282
Cdd:pfam04616 135 dprhgHGGIYLQELDNDGLKLVGPVTKLIYPGTRWvggkvtEGPHLYKRNGYYYLTYAAGGTGGPYAVGV----ARSRSP 210
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  283 TGPFEDRTgallldprvgGTPVLHQNG--NRWIGTGHVTSAVDATGQRWMLYHAVDrtdpyVAGGGTYTKRPVLMDALDW 360
Cdd:pfam04616 211 LGPYEWHP----------GNPILTSRSpeNPIYGPGHASLVETPDGEWWIVYHAGR-----PGDGGYGLGRETRIQPVEW 275

                  ....*.
gi 516970009  361 -QDGWP 365
Cdd:pfam04616 276 rADGWP 281
GH43_HoAraf43-like cd08991
Glycosyl hydrolase family 43 protein such as Halothermothrix orenii H 168 ...
57-369 7.36e-37

Glycosyl hydrolase family 43 protein such as Halothermothrix orenii H 168 alpha-L-arabinofuranosidase (HoAraf43;Hore_20580); This glycosyl hydrolase family 43 (GH43) subgroup includes Halothermothrix orenii H 168 alpha-L-arabinofuranosidase (EC 3.2.1.55) (HoAraf43;Hore_20580). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. This GH43_ HoAraf43-like subgroup includes enzymes that have been annotated as having xylan-digesting beta-xylosidase (EC 3.2.1.37) and xylanase (endo-alpha-L-arabinanase, EC 3.2.1.8) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350105 [Multi-domain]  Cd Length: 283  Bit Score: 139.62  E-value: 7.36e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  57 ADPDVIRSEDpsdpVWYLYCTrdvidsSLRNPDGslvfssIPTYRSADLVHWSFVGEALpTKPAWIGDGDMWAPDVAYVG 136
Cdd:cd08991    1 ADPFVLKHNG----TYYLYGT------GGDDGRG------FKVYVSDDLVNWEYPGGAL-EEPGLWGTKGFWAPEVFYYN 63
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 137 GRYLLYYTATNtvaPGGGSAIGVATSASPAGPWVDSGGPvvePMPSPGSA-DPAdrrwVF-DPElvtagGEHYLYF---- 210
Cdd:cd08991   64 GKFYMYYSANG---GDHGEHIAVAVSDSPLGPFRDKGKL---LIPAGGFSiDAH----VFiDDD-----GKWYLYYvrdd 128
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 211 --GSYYGGLSVRRLSADgLHSDPASQVQVAI----GNRY---------EGTHVIEHDGWFYLLGSAtNCCSGPltGYGVF 275
Cdd:cd08991  129 lgGEPGNRIYVAELEDD-LSLIGEPTLVLCPtadeRWEYgegrdwhttEGPTVLKHNGTYYLTYSA-NHFRSP--DYAVG 204
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 276 AGRSTSPTGPFEdrtgallldpRVGGTPVLHQNGNRWIGTGHVTSAVDATGQRWMLYHAVDRTDpyvagggTYTKRPVLM 355
Cdd:cd08991  205 YATADSPLGPWT----------KYEGNPILSRNDGGVNGPGHNSVFKDPDGDLYIVYHTHDSDE-------TVEPRKMRI 267
                        330
                 ....*....|....
gi 516970009 356 DALDWQDGWPVVRG 369
Cdd:cd08991  268 DRLRFDGDKLSVLG 281
GH43_CjArb43A-like cd18830
Glycosyl hydrolase family 43 protein such as Cellvibrio japonicus Ueda107 endo-alpha-1, ...
58-341 4.71e-36

Glycosyl hydrolase family 43 protein such as Cellvibrio japonicus Ueda107 endo-alpha-1,5-L-arabinanase / exo-alpha-1,5-L-arabinanase 43A (ArbA;CJA_0805) (Arb43A); This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes annotated with alpha-L-arabinofuranosidase (ABF; EC 3.2.1.55) and endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities, and includes the bifunctional Cellvibrio japonicus Ueda107 endo-alpha-1,5-L-arabinanase / exo-alpha-1,5-L-arabinanase 43A (ArbA;CJA_0805) (Arb43A). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. Many of these enzymes such as the Bacillus subtilis arabinanase Abn2, that hydrolyzes sugar beet arabinan (branched), linear alpha-1,5-L-arabinan and pectin, are different from other arabinases; they are organized into two different domains with a divalent metal cluster close to the catalytic residues to guarantee the correct protonation state of the catalytic residues and consequently the enzyme activity. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350151 [Multi-domain]  Cd Length: 291  Bit Score: 137.41  E-value: 4.71e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  58 DPDVIRSEDpsdpVWYLYCTRdvidsslrnpdgslvfSSIPTYRSADLVHWSFVGEALPTKPAWIGD------GDMWAPD 131
Cdd:cd18830    3 DPVMAREGG----TYYLFSTG----------------PGISVMSSKDLKNWTQERPVFDEPPQWAKEavpgfnGHIWAPD 62
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 132 VAYVGGRYLLYY----TATNTvapgggSAIGVAT-----SASPAGPWVDSGgPVVEPMPSPGSADPADRRWVFDPElvta 202
Cdd:cd18830   63 ISFHNGRYYLYYscsaFGKNT------SAIGVATnktldPDSPDYKWEDHG-MVVQSVPGRDLWNAIDPNVIVDEK---- 131
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 203 gGEHYLYFGSYYGGLSVRRLSADgLHSDPASQVQVAIGNRY---------------EGTHVIEHDGWFYLLGSATNCCSG 267
Cdd:cd18830  132 -GTPWLSFGSFWGGIKLVKLDPD-LKSLAEPQEWHTIARRErtfkltdseagpgaiEAPFIFKKGGYYYLFVSWDYCCRG 209
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 516970009 268 PLTGYGVFAGRSTSPTGPFEDRTGALLLDprvGGTPVLHQNGNRWIGTGHvTSAVDATGQRWMLYHAVDRTDPY 341
Cdd:cd18830  210 VNSTYKVVVGRSKNVTGPYLDKDGKSMLQ---GGGTLVVGGNKRWAGVGH-NSVYTFDGKDYLVFHAYDAADNG 279
GH43_bXyl-like cd09004
Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 ...
57-333 1.14e-32

Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (BT3675;BT_3675) and (BT3662;BT_3662); includes mostly xylanases; This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes that have been annotated as xylan-digesting beta-xylosidase (EC 3.2.1.37) and xylanase (endo-alpha-L-arabinanase, EC 3.2.1.8) activities, as well the Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (EC 3.2.1.55) (BT3675;BT_3675) and (BT3662;BT_3662). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350118 [Multi-domain]  Cd Length: 266  Bit Score: 126.96  E-value: 1.14e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  57 ADPDVIRSEDPsdpvWYLYCTRDVIDSSlrnpdGSLVFSsipTYRSADLVHWSFVGEALP-TKPAWIGDGDMWAPDVAYV 135
Cdd:cd09004    1 ADPDIVVFGGR----YYIYPTTDGPPGW-----SSTSFH---VFSSTDLVNWTDHGIILDlANDVWWANKGAWAPAVAER 68
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 136 GGRYLLYYTAtntvapggGSAIGVATSASPAGPWVDSGGPVVEPMPSPGSA-DPAdrrwVFdpelVTAGGEHYLYFGSyy 214
Cdd:cd09004   69 NGKYYFYFSA--------GSQIGVAVSDSPTGPFTDLGRPLVTGGDYGGQAiDPM----VF----VDDDGQAYLYWGN-- 130
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 215 GGLSVRRLSADGLHSDPASQVQVAIGNRYEGTHVIEHDGWFYLLGSATNCCSGpltGYGVFAGRSTSPTGPFEDRTGALL 294
Cdd:cd09004  131 GTAYVARLNDDMVSFDGEVVVSITPPNFREGPFVHKRNGIYYLSWSENDTRDP---DYRVRYATSDSPLGPWTYRGVGLL 207
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|.
gi 516970009 295 LDPRVGGTpvlhqngnrwiGTGH--VTsAVDATGQRWMLYH 333
Cdd:cd09004  208 LDSAGGIK-----------GTGHhsIV-QVPGTDEWYIAYH 236
GH43_AnAbnA-like cd18831
Glycosyl hydrolase family 43 protein such as Aspergillus niger endo-alpha-L-arabinanase (AbnA); ...
82-347 1.62e-32

Glycosyl hydrolase family 43 protein such as Aspergillus niger endo-alpha-L-arabinanase (AbnA); This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities such as Aspergillus niger AbnA, Aspergillus niveus AbnA, and Chrysosporium lucknowense Abn1. It belongs to the GH43_Arb43a subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43_Arb43a subgroup includes mostly enzymes with alpha-L-arabinofuranosidase (ABF; EC 3.2.1.55) and endo-alpha-L-arabinanase activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. The GH43_Arb43a subgroup includes many enzymes such as Bacillus subtilis arabinanase Abn2, that hydrolyzes sugar beet arabinan (branched), linear alpha-1,5-L-arabinan and pectin, and are different from other arabinases; they are organized into two different domains with a divalent metal cluster close to the catalytic residues to guarantee the correct protonation state of the catalytic residues and consequently the enzyme activity. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350152 [Multi-domain]  Cd Length: 286  Bit Score: 127.33  E-value: 1.62e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  82 DSSL-RNPDGSL-VFS---SIPTYRSADLV-HWSFVGEALPTkPAWI---GDGDMWAPDVAYVGGRYLLYYtATNTVapg 152
Cdd:cd18831    3 DPSIiRREDGTYfRFStggGIRIATAPSLTgPWTYVGSVLPG-GSSIdlaGNDDLWAPDVHYVNGTYYCYY-SVSTF--- 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 153 gG---SAIGVATSASP-AGPWVDSGGpvvepMPSPGSADPADRrwvFDPELVTAGGEH-YLYFGSYYGGLSVRRLSADGL 227
Cdd:cd18831   78 -GsqdSAIGVATSPTMePGSWTDHGA-----VIRSSSGDPYNA---IDPNLIVDDDGTpYLTFGSYWQGIFQVPLTDPLL 148
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 228 hSDPASQVQVAIGNRYEGTHVIE------HDGWFYLLGSATNCC----SGPLTG--YGVFAGRSTSPTGPFEDRTGALLL 295
Cdd:cd18831  149 -SPAAGPPPTHLAYNPSGNHPEEgsfmykHGGYYYLFFSSGICCgydpSLPAPGeeYKIRVCRSTSPTGPFVDKDGRDCL 227
                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|..
gi 516970009 296 DPrvGGTPVLHQNGNRWIGTGHVTSAVDATGQRWMLYHAVDRTDPYVAGGGT 347
Cdd:cd18831  228 EG--GGTTVLLASHGNVYAPGGQGVYDDPVGGDVLYYHYVNTTIGYADGDAQ 277
GH43_BsArb43A-like cd18829
Glycosyl hydrolase family 43 protein such as Bacillus subtilis subsp. subtilis str. 168 ...
56-339 1.29e-31

Glycosyl hydrolase family 43 protein such as Bacillus subtilis subsp. subtilis str. 168 endo-alpha-1,5-L-arabinanase Arb43A; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes annotated as having endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities, and includes Bacillus subtilis subsp. subtilis str. 168 endo-alpha-1,5-L-arabinanase (AbnA;BSU28810) (Arb43A). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the arabinofuranosidase (ABF; EC 3.2.1.55) enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. Many of these enzymes such as the Bacillus subtilis arabinanase Abn2, that hydrolyzes sugar beet arabinan (branched), linear alpha-1,5-L-arabinan and pectin, are different from other arabinases; they are organized into two different domains with a divalent metal cluster close to the catalytic residues to guarantee the correct protonation state of the catalytic residues and consequently the enzyme activity. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350150 [Multi-domain]  Cd Length: 273  Bit Score: 124.40  E-value: 1.29e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  56 CADPDVIRSEDPsdpvWYLYCTRDvidsslrnpdgslvfsSIPTYRSADLVHWSFVGEALPTKPAWIG-------DGDMW 128
Cdd:cd18829    1 THDPSIIKEGST----WWTFSTGD----------------GIPVKYSSDGLNWTQGPPIFGSPLSWWKtyvpantTNDVW 60
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 129 APDVAYVGGRYLLYYT----ATNTvapgggSAIGVATSASPA-GPWVDSGGPVvepmpspgSADPADRRWVFDPELVT-A 202
Cdd:cd18829   61 APDVHYYNGKYWLYYAistfGSNT------SAIGLASASSIAaGNWTDEGLVL--------RSTSADNYNAIDPNLVIdA 126
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 203 GGEHYLYFGSYYGGLSVRRLSADGLHSDpASQVQVA--IGNRYEGTHVIEHDGWFYLLGSATNCCSGPLTGYGVFAGRST 280
Cdd:cd18829  127 SGNPWLVFGSFWSGIKITRLDKATMKPT-GSIYSIAsrPSGGIEGPFIVYRDGYYYLFVSIDKCCRGVNSTYKIAYGRST 205
                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 516970009 281 SPTGPFEDRTGALLLDprvGGTPVLHQNGNRWIGTGHvtSAVDATGQRWMLYHAVDRTD 339
Cdd:cd18829  206 SITGPYLDKNGKDMLN---GGGTVLDSGNSRWKGPGG--QDIYATGKNVIARHAYDANN 259
GH43_AXH_like cd08990
Glycosyl hydrolase family 43 protein, includes arabinoxylan arabinofuranohydrolase, ...
66-363 1.11e-30

Glycosyl hydrolase family 43 protein, includes arabinoxylan arabinofuranohydrolase, beta-xylosidase, endo-1,4-beta-xylanase, and alpha-L-arabinofuranosidase; This subgroup includes Bacillus subtilis arabinoxylan arabinofuranohydrolase (XynD;BsAXH-m23;BSU18160), Butyrivibrio proteoclasticus alpha-L-arabinofuranosidase (Xsa43E;bpr_I2319), Clostridium stercorarium alpha-L-arabinofuranosidase XylA, and metagenomic beta-xylosidase (EC 3.2.1.37) / alpha-L-arabinofuranosidase (EC 3.2.1.55) CoXyl43. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. The GH43_AXH-like subgroup includes enzymes that have been characterized with beta-xylosidase, alpha-L-arabinofuranosidase, endo-alpha-L-arabinanase as well as arabinoxylan arabinofuranohydrolase (AXH) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. AXHs specifically hydrolyze the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan. Metagenomic beta-xylosidase/alpha-L-arabinofuranosidase CoXyl43 shows synergy with Trichoderma reesei cellulases and promotes plant biomass saccharification by degrading xylo-oligosaccharides, such as xylobiose and xylotriose, into the monosaccharide xylose. Studies show that the hydrolytic activity of CoXyl43 is stimulated in the presence of calcium. Several of these enzymes also contain carbohydrate binding modules (CBMs) that bind cellulose or xylan. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350104 [Multi-domain]  Cd Length: 269  Bit Score: 121.55  E-value: 1.11e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  66 DPSDPVW----YLYCTRDVidsslrnpDGSLVFSSIPTYR---SADLVHWSFVGEALPTK--PAWiGDGDMWAPDVAYVG 136
Cdd:cd08990    2 DPAAHVFngkvYVYASHDE--------APANGYFIMDDWHvfsSTDLVNWTDHGEILPPDdvFWW-ASGNAWAPDAVYKN 72
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 137 GRYLLYYTATNTvapGGGSAIGVATSASPAGPWVDSGGPvvePMPSPGSADPadrrWVFDPE-LVTAGGEHYLYFGS--- 212
Cdd:cd08990   73 GKYYFYFPVGQA---SDGFGIGVAVSDSPAGPFKDALGK---PLIPEGLNGI----EGIDPAvFVDDDGRAYLYFGGggg 142
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 213 YYGGlsvrRLSADGLHSDPASQVQVAIG--NRYEGTHVIEHDGWFYLLgSATNCCSGPLTGYGVfagrSTSPTGPFEDRT 290
Cdd:cd08990  143 YYVA----KLKDDMISLAGEPQKIKNGGlkGFFEAPWVFKRNGTYYLS-YAGGWAYPAEIAYST----ADSPLGPYTYRG 213
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 516970009 291 gallldprvggtpVLHQNGNrwIGTGHvTSAVDATGQRWMLYHAVDrtdpyvAGGGTYTKRPVLMDALDW-QDG 363
Cdd:cd08990  214 -------------VILDPVG--SGTNH-GSIVEFKGQWYLFYHTAD------LSGGGDFRRSVCIDYLHYnADG 265
GH43_XYL-like cd09002
Glycosyl hydrolase family 43, beta-D-xylosidase (uncharacterized); This glycosyl hydrolase ...
92-367 1.50e-30

Glycosyl hydrolase family 43, beta-D-xylosidase (uncharacterized); This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes that have been annotated as having beta-1,4-xylosidase (beta-D-xylosidase;xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity. They are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350116 [Multi-domain]  Cd Length: 271  Bit Score: 121.18  E-value: 1.50e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  92 LVFSSIPTYR------SADLVHWSFVGEALPTKpawigDGDMWAPDVAYVGGRYLLYYtatntvaPGGGSAIGVATSASP 165
Cdd:cd09002   24 MTHSSFDYYPglliwhSRDLVNWEPIGAALTEY-----IGTVWAPDLIKHDGRYYIYF-------PAKGGTNYVITADDI 91
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 166 AGPWVDsggPVvePMPSPGSADPadrrwvfdPELVTAGGEHYLYFGsyyGGLSVRrLSADGLHSDPASQvQVAIGNRY-- 243
Cdd:cd09002   92 AGPWSE---PI--DLKVGSGIDP--------GHVVDEDGKRYLFLS---GGRRVR-LTDDGLSVAGPPE-KVYDGWRYpd 153
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 244 ---------EGTHVIEHDGWFYLLgSATNCCSGPLTGYGVFAGRSTSPTGPFEDrtgalllDPRvggTPVLH--QNGNRW 312
Cdd:cd09002  154 ewdvecfclEGPKLFRRGGYYYLT-TAQGGTAGPPTSHMVVSARSKSPHGPWEN-------SPY---NPLVRtqSREEKW 222
                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 516970009 313 IGTGHVTSAVDATGQRWMLYHAVDrtdpyvagGGTYT-KRPVLMDALDW-QDGWPVV 367
Cdd:cd09002  223 WSKGHGTLVEGPDGKWWMVYHGYE--------NGYRTlGRQTLLEPVEWtADGWFRI 271
GH_F cd08978
Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F; This glycosyl hydrolase clan F ...
57-334 4.22e-25

Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F; This glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) includes family 43 (GH43) and 62 (GH62). GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH62 includes enzymes characterized as arabinofuranosidases (alpha-L-arabinofuranosidases; EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose side chains from xylans. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. GH62 are also predicted to be inverting enzymes. A common structural feature of both, GH43 and GH62 enzymes, is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350092 [Multi-domain]  Cd Length: 251  Bit Score: 104.83  E-value: 4.22e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  57 ADPDVIRSEDpsdpVWYLYCTRDVIDSSLRnpdgslvfssIPTYRSADLVHWSFVGEALP--TKPAWiGDGDMWAPDVAY 134
Cdd:cd08978    1 ADPSILKDNG----RYYIYATTDDTGTGTG----------IVVWKSKDLVNWKEEGTVLSrgKSKSW-GTGNLWAPEVYY 65
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 135 VG-GRYLLYYTatnTVAPGGGSAIGVATSASPAGPWVDsggpvvepmpsPGSADPADRRWV-FDPELVTAGGEH-YLYFG 211
Cdd:cd08978   66 FNsGKWYLYYS---AVPNGGGGRIYVATSDSPEGPFTP-----------IVSGKLGDRGSGsIDPTVFVDDDGKlYLYYG 131
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 212 S--YYGGLSVRRLSADGLHSDPASQVQVAI-------GNRYEGTHVIEHDGWFYLLGSAtNCCSGPltgYGVFAGRSTSP 282
Cdd:cd08978  132 DedDSGDIYVAELDPDLLTIKGDVTLLIGEvvgsgfrGNYFEGPAVFKRNGYYYLIYSA-GGTDGG---YAIGYATSDSP 207
                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|...
gi 516970009 283 TGPFEDRtgallldprvGGTPVLHQNGNRWI-GTGHVTSAVDATGQRWMLYHA 334
Cdd:cd08978  208 LGPWEKA----------SHNPGLQTSGATGIyGPGHGSIFQDEGDRWYIVYHA 250
GH43_FsAxh1-like cd09001
Glycosyl hydrolase family 43 such as Fibrobacter succinogenes subsp. succinogenes S85 ...
38-367 2.82e-24

Glycosyl hydrolase family 43 such as Fibrobacter succinogenes subsp. succinogenes S85 arabinoxylan alpha-L-arabinofuranosidase; This glycosyl hydrolase family 43 (GH43) includes mostly enzymes that have been annotated as having beta-1,4-xylosidase (beta-D-xylosidase; xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity. They are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. This subfamily includes the characterized Clostridium stercorarium F-9 beta-xylosidase Xyl43B. It also includes Humicola insolens AXHd3 (HiAXHd3), a GH43 arabinofuranosidase (EC 3.2.1.55) that hydrolyzes O3-linked arabinose of doubly substituted xylans, a feature of the polysaccharide that is recalcitrant to degradation. It possesses an additional C-terminal beta-sandwich domain such that the interface between the domains comprises a xylan binding cleft that houses the active site pocket. The HiAXHd3 active site is tuned to hydrolyze arabinofuranosyl or xylosyl linkages, and the topology of the distal regions of the substrate binding surface confers specificity. It also includes Fibrobacter succinogenes subsp. succinogenes S85 arabinoxylan alpha-L-arabinofuranosidase (Axh1;Fisuc_1769;FSU_2269), Paenibacillus sp. E18 alpha-L-arabinofuranosidase (Abf43A), Bifidobacterium adolescentis ATCC 15703 double substituted xylan alpha-1,3-L-specific arabinofuranosidase d3 (AXHd3;AXH-d3;BaAXH-d3;BAD_0301;E-AFAM2), and Chrysosporium lucknowense C1 arabinoxylan hydrolase / double substituted xylan alpha-1,3-L-arabinofuranosidase (Abn7;AXHd). A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350115 [Multi-domain]  Cd Length: 270  Bit Score: 102.98  E-value: 2.82e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  38 TYSNPLpldlgggkLAEQCADPDVIRSEDpsdpVWYLYCTrdvidsslrnpdgSLVFS-SIPTYRSADLVHWSFVGEALP 116
Cdd:cd09001    1 TYTNPV--------LWADYPDPDVIRVGD----TYYMVSS-------------TMHFSpGAPILHSKDLVNWEIVGYVVD 55
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 117 TkpawIGDGD--------------MWAPDVAYVGGRYLLYYTatntvAPGGGsaIGVATSASPAGPWVDSggpvvePMPS 182
Cdd:cd09001   56 R----LDDGDayyledgknaygkgIWAPSLRYHNGKFYVYFC-----TNTGG--TYVYTADDPAGPWSRP------ALIG 118
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 183 PGSADPAdrrWVFDPElvtagGEHYLYFGsyYGGLSVRRLSADGLHSDPASQVQV---AIGNRYEGTHVIEHDGWFYLLg 259
Cdd:cd09001  119 KGYHDPS---LLFDDD-----GKAYLVYG--NGEIRLTELSPDGTGVGGEGRVIIdgtEEGLGAEGSHLYKINGYYYIF- 187
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 260 satnCCSGPLTGYGVFAGRSTSPTGPFEDRTgallldprvggtpVLHQNGNRWIGTGHVTSAVDATGQRWMLYHAVDRtd 339
Cdd:cd09001  188 ----NIEWGGGGRTQVVLRSKSLYGPYEGRV-------------VLDDGSGTGDNGPHQGGLVDTPDGEWWFMLFQDR-- 248
                        330       340
                 ....*....|....*....|....*...
gi 516970009 340 pyvaggGTYTKRPVLMdALDWQDGWPVV 367
Cdd:cd09001  249 ------GAVGRIPVLV-PVTWKDGWPVI 269
GH43_BT3675-like cd18828
Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 ...
57-364 2.61e-23

Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (BT3675;BT_3675); This glycosyl hydrolase family 43 (GH43) subgroup includes the Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (EC 3.2.1.55) (BT3675;BT_3675) and (BT3662;BT_3662). It belongs to the GH43_bXyl subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. The GH43_bXyl subgroup also includes enzymes annotated as having xylan-digesting beta-xylosidase (EC 3.2.1.37) and xylanase (endo-alpha-L-arabinanase, EC 3.2.1.8) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350149 [Multi-domain]  Cd Length: 283  Bit Score: 100.43  E-value: 2.61e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  57 ADPDVIRSEDpsdpVWYLYCTRDvidsSLRNPDGSL--VFSSiptyrsADLVHWSFVGEALPTK---PAWIGDGDMWAPD 131
Cdd:cd18828    1 ADPDIAYFDG----KYYIYPTTD----GFPGWSGTQfhVFSS------DDLVTWKDEGVILDLKndqVVPWATGNAWAPT 66
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 132 VAYVGGRYLLYYTATNtvaPGGGSAIGVATSASPAGPWVDSGGPVVEPMPSPGSA----DPAdrrwVF-DPElvtaGGEH 206
Cdd:cd18828   67 IEERDGKYYFYFCGKN---PDGRSQIGVAVADSPTGPFTAQGSPLITHEMARVTMgqaiDPS----VFtDPV----DGKY 135
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 207 YLYFGSYYGglSVRRLSADGLHSDPASQVQVAIGNRY-EGTHVIEHDGWFYLLGSATNccsgplTG---YGVFAGRSTSP 282
Cdd:cd18828  136 YLYWGNGYA--AIAELNDDMISIKPGTLVNLDGLTDFrEAVTVLYRDGLYHFTWSCDD------TGsenYHVNYGTSDSP 207
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 283 TGPFEDRtgallldprvggTPVLHQNGNRWI-GTGH-VTSAVDATGQRWMLYHAVdRTDPYVAGGGTYTKRPVLMDALDW 360
Cdd:cd18828  208 YGPITYR------------GVILQKDPSKGIlGTGHhSILQVPGTDEWYIAYHRF-ATPLGIYGSGLGYHRETCIDRLTF 274

                 ....*
gi 516970009 361 -QDGW 364
Cdd:cd18828  275 dADGL 279
GH43_XynD-like cd09003
Glycosyl hydrolase family 43 protein such as Bacillus subtilis arabinoxylan ...
73-334 1.36e-22

Glycosyl hydrolase family 43 protein such as Bacillus subtilis arabinoxylan arabinofuranohydrolase (XynD;BsAXH-m23;BSU18160); This glycosyl hydrolase family 43 (GH43) subgroup includes characterized Bacillus subtilis arabinoxylan arabinofuranohydrolase (AXH), Caldicellulosiruptor sp. Tok7B.1 beta-1,4-xylanase (EC 3.2.1.8) / alpha-L-arabinosidase (EC 3.2.1.55) XynA, Caldicellulosiruptor sp. Rt69B.1 xylanase C (EC 3.2.1.8) XynC, and Caldicellulosiruptor saccharolyticus beta-xylosidase (EC 3.2.1.37)/ alpha-L-arabinofuranosidase (EC 3.2.1.55) XynF. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. It belongs to the GH43_AXH-like subgroup which includes enzymes that have been annotated as having beta-xylosidase, alpha-L-arabinofuranosidase and arabinoxylan alpha-L-1,3-arabinofuranohydrolase, xylanase (endo-alpha-L-arabinanase) as well as AXH activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. AXHs specifically hydrolyze the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan. Bacillus subtilis AXH (BsAXH-m2,3) has been shown to cleave arabinose units from O-2- or O-3-mono-substituted xylose residues and superposition of its structure with known structures of the GH43 exo-acting enzymes, beta-xylosidase and alpha-L-arabinanase, each in complex with their substrate, reveals a different orientation of the sugar backbone. Several of these enzymes also contain carbohydrate binding modules (CBMs) that bind cellulose or xylan. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350117 [Multi-domain]  Cd Length: 315  Bit Score: 99.26  E-value: 1.36e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  73 YLYCTRDVIDSSLRNPDGSLVFSSIPTY---RSADLVHWSFVGE---ALPTKPA-WIGDGdmWAPDVAY----VGGRYLL 141
Cdd:cd09003   22 YVYGTNDDQQYNANGKKKDNSYYNINSLtviSSDDMVNWTDHGEipvAGPNGIAkWAGNS--WAPSVAYkninGKDKFYL 99
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 142 YYtatntvaPGGGSAIGVATSASPAGPWVDsggPVVEPMPSPGSADPADRRWVFDPE-LVTAGGEHYLYFG--------S 212
Cdd:cd09003  100 YF-------ANGGGGIGVLTADSPTGPWTD---PLGKPLITRSTPGCAGVVWLFDPAvFIDDDGQGYLYFGggvpggseA 169
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 213 YYGGLSVRRLSADGLHSDPASQVQVAIgNRYEGTHVIEHDGWFYLLGSaTNCCSGPLTGYgVFAGR-----STSPTGPFE 287
Cdd:cd09003  170 NPKTARVIKLGDDMISVDGSAVTIDAP-YFFEASGINKINGKYYYSYC-TNFSGRDDPAY-PGAGSiaymtSDNPMGPFT 246
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*..
gi 516970009 288 DRtGALLLDPrvgGTpvlhqNGNRWiGTGHvTSAVDATGQRWMLYHA 334
Cdd:cd09003  247 YK-GVILKNP---GT-----FFGNG-GNNH-HSIFEFKGKWYIFYHA 282
GH43_XYL-like cd08989
Glycosyl hydrolase family 43, beta-D-xylosidases and arabinofuranosidases; This glycosyl ...
89-361 1.16e-21

Glycosyl hydrolase family 43, beta-D-xylosidases and arabinofuranosidases; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes that have been annotated as having beta-1,4-xylosidase (beta-D-xylosidase;xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity, including Selenomonas ruminantium beta-D-xylosidase SXA. These are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. It also includes various GH43 family GH43 arabinofuranosidases (EC 3.2.1.55) including Humicola insolens alpha-L-arabinofuranosidase AXHd3, Bacteroides ovatus alpha-L-arabinofuranosidase (BoGH43, XynB), and the bifunctional Phanerochaete chrysosporium xylosidase/arabinofuranosidase (Xyl;PcXyl). GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350103 [Multi-domain]  Cd Length: 272  Bit Score: 95.50  E-value: 1.16e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  89 DGSLVFSS------IPTYRSADLVHWSFVGEALPT------KPAWIGDGdMWAPDVAYVGGRYLLYYTATNTVAPGGGSA 156
Cdd:cd08989   19 DYYMVNSTfqyfpgIPISHSKDLVHWTPIGHALTRpeqldlTGGPDGGG-IWAPDISYHDGKFYIYYTVVLNVGSWKGRR 97
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 157 IGVATSASPAGPW-----VDSGGpvvepmpspgsadpadrrwvFDPEL-VTAGGEHYLYFGsyYGGLSVRRLSADGLH-S 229
Cdd:cd08989   98 NYLVTSEDPEGPWsepvwLDEGG--------------------IDPSLfVDDDGKHYMLLN--PGGIRLAELNPDCTKqI 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 230 DPASQVQVAIGNRY-EGTHVIEHDGWFYLLGSAtnccSGPLTGYGVFAGRSTSPTGPFEDrtgalllDPRvggTPVLHQN 308
Cdd:cd08989  156 GEPKRIWEGTGGRApEGPHLYKKDGYYYLLTAE----GGTGYGHAITIARSKTIYGPYEP-------CPY---NPILRQQ 221
                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 516970009 309 G--NRWIGTGHVTSAVDATGQRWMLYHavdrTDPYVAGGGTYTKRPVLMDALDWQ 361
Cdd:cd08989  222 DpqAPLQRCGHGKLVETPDGEWWMVYL----CGRPLPGGYCPLGRETALAPVEWT 272
GH43_F5-8_typeC-like cd18608
Glycosyl hydrolase family 43 protein most having a F5/8 type C domain C-terminal to the GH43 ...
57-358 3.25e-21

Glycosyl hydrolase family 43 protein most having a F5/8 type C domain C-terminal to the GH43 domain; This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes that have been annotated as having beta-xylosidase (EC 3.2.1.37), xylanase (EC 3.2.1.8), and beta-galactosidase (EC 3.2.1.145) activities, and some as F5/8 type C domain (also known as the discoidin (DS) domain)-containing proteins. Most contain a F5/8 type C domain C-terminal to the GH43 domain. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. Characterized enzymes belonging to this subgroup include Lactobacillus brevis (LbAraf43) and Weissella sp (WAraf43) which show activity with similar catalytic efficiency on 1,5-alpha-L-arabinooligosaccharides with a degree of polymerization (DP) of 2-3; size is limited by an extended loop at the entrance to the active site. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350120 [Multi-domain]  Cd Length: 276  Bit Score: 94.27  E-value: 3.25e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  57 ADPDVIRSEDpsdpVWYLYCTRDvidsslrnPDGSLVFSSIPTYRSADLVHWSFVGEALPTKpAWIGDGDMWAPDVAY-V 135
Cdd:cd18608    2 ADPSIVKFGG----TYYLYATTD--------GWGGFNSGEPVVWKSKDFVNWKFEGLNWPTK-AASGDSKVWAPSVVKgK 68
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 136 GGRYLLYYTAtntvapggGSAIGVATSASPAGPWVDSGG---PVVEPMPSPGSADpadrrwvFDPE-LVTAGGEHYLYFG 211
Cdd:cd18608   69 DGKYYMYVSV--------GSEIYVGVADSPLGPWKNANGdgpPIIPGDGKPNYHM-------IDAEpFIDDDGKAYLYWG 133
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 212 S---YYGGLSVRRLSADGLHSDPASQVQVAIGNRYEGTHVIEHDGWFYLLGSATNCcsgPLTGYGVFAGRSTSPTGPFED 288
Cdd:cd18608  134 SglhVNGHCFAAKLNPDMVTFDGSEPTIVTPRDYFEAPFMFKRNGIYYLMYSGGGC---WDETYNVRYAVSDNPLGPFEE 210
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 516970009 289 rtgallldprVGGTPVLHQN-GNRWIGTGHvTSAVDATGQRWMLYHAVDRTDPyvaggGTYTKRPVLMDAL 358
Cdd:cd18608  211 ----------GENSPILQTDeAKGIFGPGH-HSVFEEGGQYYILYHRQGYPFS-----PGGTLRQVCVDEL 265
GH43_XylA-like cd18620
Glycosyl hydrolase family 43-like protein such as Clostridium stercorarium ...
104-287 2.91e-20

Glycosyl hydrolase family 43-like protein such as Clostridium stercorarium alpha-L-arabinofuranosidase XylA; This glycosyl hydrolase family 43 (GH43) subgroup belongs to the GH43_AXH-like subgroup which includes enzymes that have been characterized with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55), alpha-1,2-L-arabinofuranosidase 43A (arabinan-specific; EC 3.2.1.-), endo-alpha-L-arabinanase as well as arabinoxylan arabinofuranohydrolase (AXH) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. The GH43_XylA-like subgroup includes Clostridium stercorarium alpha-L-arabinofuranosidase XylA, and enzymes that have been annotated as having beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55), endo-alpha-L-arabinanase (EC 3.2.1.-) as well as arabinoxylan arabinofuranohydrolase (AXH) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. AXHs specifically hydrolyze the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan.


Pssm-ID: 350132 [Multi-domain]  Cd Length: 274  Bit Score: 91.12  E-value: 2.91e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 104 DLVHWSFVGEALPTK--PAWIGDGD--MWAPDVAYVGGRYLLYYtatntvAPGGGSAIGVATSASPAGPWVDsGGPVVEP 179
Cdd:cd18620   40 DLSNWRYHGVIFRSDqdPDEVPPGKglLYAPDVVKGPGRYYLYY------CLSKGSVEGVAVSDSPAGPFEY-LGPVKYP 112
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 180 MPSPGSAdpadrrwvFDPE-LVTAGGEHYLYFGsyYGGLSVRRLSADGLHSDPASQVQVAIGNRYEGTHVIE------HD 252
Cdd:cd18620  113 RKGDIFQ--------IDPAvLVDDDGRVYLYWG--QGGSKGAELDPDMLTIKPETIVDVPAGITFEGHGFFEgssirkIN 182
                        170       180       190
                 ....*....|....*....|....*....|....*
gi 516970009 253 GWFYLLGSATNCCSGPLTGYGVfagrSTSPTGPFE 287
Cdd:cd18620  183 GIYYLVYSSISRGRPTELCYAT----SKSPLGPFT 213
GH43_XynB-like cd18617
Glycosyl hydrolase family 43, such as Bacteroides ovatus alpha-L-arabinofuranosidase (BoGH43, ...
87-367 3.60e-19

Glycosyl hydrolase family 43, such as Bacteroides ovatus alpha-L-arabinofuranosidase (BoGH43, XynB); This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes that have been characterized to have alpha-L-arabinofuranosidase (EC 3.2.1.55) and beta-1,4-xylosidase (beta-D-xylosidase;xylan 1,4-beta-xylosidase; EC 3.2.1.37) activities. Beta-1,4-xylosidases are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Also included in this subfamily are Bacteroides ovatus alpha-L-arabinofuranosidases, BoGH43A and BoGH43B, both having a two-domain architecture, consisting of an N-terminal 5-bladed beta-propeller domain harboring the catalytic active site, and a C-terminal beta-sandwich domain. However, despite significant functional overlap between these two enzymes, BoGH43A and BoGH43B share just 41% sequence identity. The latter appears to be significantly less active on the same substrates, suggesting that these paralogs may play subtly different roles during the degradation of xyloglucans from different sources, or may function most optimally at different stages in the catabolism of xyloglucan oligosaccharides (XyGOs), for example before or after hydrolysis of certain side-chain moieties. It also includes Phanerochaete chrysosporium BKM-F-1767 Xyl, a bifunctional xylosidase/arabinofuranosidase. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350129 [Multi-domain]  Cd Length: 285  Bit Score: 88.33  E-value: 3.60e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  87 NPDGS---------LVFSS------IPTYRSADLVHWSFVGEALpTKPAWI------GDGDMWAPDVAYVGGRYllYYTA 145
Cdd:cd18617    8 YPDPSicrvgddyyLVTSSfeyfpgLPIYHSKDLVNWELIGHAL-DRPSQLdlrgvpSSGGIFAPTIRYHDGRF--YIIT 84
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 146 TNTVAPGGGSAIgvATSASPAGPWVDsggPVVepMPSPGsadpadrrwvFDPELvtaggehylYF---GSYYggLSVRRL 222
Cdd:cd18617   85 TNVSTDGRGNFI--VTADDPAGPWSD---PVW--LDGPG----------IDPSL---------FFdddGKVY--LTGTGP 136
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 223 SADGLH------------------SDPASQVQVAIGNRY-EGTHVIEHDGWFYLL----GsatnccsgplTGYG--VFAG 277
Cdd:cd18617  137 PPDPYEghggiwqqeidletgkllGEPKVLWNGGTGGRWpEGPHLYKIDGWYYLLiaegG----------TEEGhsETIA 206
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 278 RSTSPTGPFEDrtgalllDPrvgGTPVLHQ--NGNRWI-GTGHVTSAVDATGQRWMLYHAVdRtdPYvAGGGTYTKRPVL 354
Cdd:cd18617  207 RSRSPWGPYEP-------CP---NNPILTHrhLGSNPVqNTGHADLVEDPDGSWWAVFLGV-R--PY-GGGFHNLGRETF 272
                        330
                 ....*....|...
gi 516970009 355 MDALDWQDGWPVV 367
Cdd:cd18617  273 LAPVEWEDGWPVV 285
GH43_XlnD-like cd18827
Glycosyl hydrolase family 43 protein such as Aspergillus niger DMS1957 xylanase D (XlnD); ...
100-341 5.34e-19

Glycosyl hydrolase family 43 protein such as Aspergillus niger DMS1957 xylanase D (XlnD); includes mostly xylanases; This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes that have mostly been annotated as xylanases (endo-alpha-L-arabinanase, EC 3.2.1.8). It belongs to the GH43_bXyl-like subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. The GH43_bXyl-like subgroup includes enzymes that have been annotated as xylan-digesting beta-xylosidases (EC 3.2.1.37) and xylanases, as well the Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (EC 3.2.1.55) (BT3675;BT_3675) and (BT3662;BT_3662). GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350148 [Multi-domain]  Cd Length: 277  Bit Score: 87.72  E-value: 5.34e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 100 YRSADLVHWSFVGEALPTKP-AWIGDGdMWAPDVAYVGGRYLLYYTATNTVAPGGGSAIGVATSASPAGPWVDS-GGPVV 177
Cdd:cd18827   31 FSSPDLVHWTKHERILDMADvPWANRA-VWAPSVIEKNGKYYLYFAANDIQSDDEGGGIGVAVADRPEGPFKDAlGKPLI 109
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 178 EPMpsPGSADPADRRWVFDPElvtagGEHYLYFGSyYGGLSVRRLSAD--GLHSDPASQVQVAI--GNRYEGTHVIEHDG 253
Cdd:cd18827  110 GEF--HNGAQPIDQHVFKDDD-----GQAYLYYGG-WGHCNVAKLNDDmtSLVPFDDGETFKEItpEGYVEGPFMFKRNG 181
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 254 WFYLLGSAtnccsGPLTG--YGVFAGRSTSPTGPFEdRTGAllldprvggtpVLHQNGNRWIGTGHVTSA-VDATGQRWM 330
Cdd:cd18827  182 KYYFMWSE-----GGWTGpdYSVAYAVADSPLGPFK-RIGK-----------ILQQDPAIATGAGHHSVVnVPGTDDWYI 244
                        250
                 ....*....|...
gi 516970009 331 LYH--AVDRTDPY 341
Cdd:cd18827  245 VYHrrPLGETDGN 257
GH43_GsAbnA-like cd18832
Glycosyl hydrolase family 43 protein such as Geobacillus stearothermophilus endo-alpha-1, ...
101-333 4.65e-18

Glycosyl hydrolase family 43 protein such as Geobacillus stearothermophilus endo-alpha-1,5-L-arabinanase AbnA; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes with alpha-L-arabinofuranosidase (ABF; EC 3.2.1.55) and endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities. It includes Geobacillus stearothermophilus T-6 NCIMB 40222 AbnA, Bacillus subtilis subsp. subtilis str. 168 (Abn2;YxiA;J3A;BSU39330) (Arb43B), and Thermotoga petrophila RKU-1 (AbnA;TpABN;Tpet_0637). These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. Many of these enzymes are different from other arabinases; they are organized into two different domains with a divalent metal cluster close to the catalytic residues to guarantee the correct protonation state of the catalytic residues and consequently the enzyme activity. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350153 [Multi-domain]  Cd Length: 332  Bit Score: 85.76  E-value: 4.65e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 101 RSADLVHWS------------------FVGEALPTKPAWIGDGDMWAPDVAY--VGGRYLLYYTATNTVAPgggSAIGVA 160
Cdd:cd18832   24 KSTDLMNWTqftngvttdnpllfnlfdSTAWELAEDFNWAGGGNLWAPDVIYnkAMGKYCMYYSVSGDDSP---SAIGLA 100
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 161 TSASPAGPWVDSgGPVVE-----PMPSPGSADPADRRW-------VFDPELVT-AGGEHYLYFGSYYGGLSVRRL----- 222
Cdd:cd18832  101 TADNIEGPYTYK-GTVLKsgftgSTSADADVYLTGGKYnnnyhpnAIDPCVFYdKDGKLWMVYGSWSGGIFLLELdpktg 179
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 223 ------SADGLHSDPASQVQVAIGN--RYEGTHVIEHD--GWFYLLGSatnccSGPLT---GYGVFAGRSTSPTGPFEDR 289
Cdd:cd18832  180 lrdysvETDGNLPDQYYGKKIAGGYhaSGEGPYILYDKdtGYYYLFVS-----YGGLDangGYNIRVFRSKNPDGPYVDA 254
                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 516970009 290 TGALLLDPR--------VGGTPVLHQNGN-RWIGT-----GHVTSAVDATGQRWMLYH 333
Cdd:cd18832  255 AGNDAIYTSgndnlglkDGGIIELMGNYKlPGLGTgyvspGHNSAYYDEDGKYYLVYH 312
GH43_Xsa43E-like cd18618
Glycosyl hydrolase family 43, including Butyrivibrio proteoclasticus arabinofuranosidase ...
66-360 1.81e-17

Glycosyl hydrolase family 43, including Butyrivibrio proteoclasticus arabinofuranosidase Xsa43E; This glycosyl hydrolase family 43 (GH43) subgroup belongs to the GH43_AXH-like subgroup which includes enzymes that have been characterized with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55), alpha-1,2-L-arabinofuranosidase 43A (arabinan-specific; EC 3.2.1.-), endo-alpha-L-arabinanase as well as arabinoxylan arabinofuranohydrolase (AXH) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. AXHs specifically hydrolyze the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan. This subgroup includes Cellvibrio japonicus arabinan-specific alpha-1,2-arabinofuranosidase, CjAbf43A, which confers its specificity by a surface cleft that is complementary to the helical backbone of the polysaccharide, and Butyrivibrio proteoclasticus GH43 enzyme Xsa43E, also an arabinofuranosidase, which has been shown to cleave arabinose side chains from short segments of xylan. Several of these enzymes also contain carbohydrate binding modules (CBMs) that bind cellulose or xylan. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350130 [Multi-domain]  Cd Length: 275  Bit Score: 83.04  E-value: 1.81e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  66 DPSDPVW----YLYCTRDV--IDSSLRNPDGSLVFSSiptyrsADLVHWSFVGEALPTKPAWIGDGDMWAPDVAYVGGRY 139
Cdd:cd18618    4 DPAALVHgdtvYLYTGHDEapPGGTFFVMNDWRVFST------TDMVNWTDHGAVLSLKDFSWAKGDAWAGQVIERNGKF 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 140 LLYYTATNTVapGGGSAIGVATSASPAGPWVDSGGPvvePMPSPGSADPADRRWV-FDPE-LVTAGGEHYLYFGSyyGGL 217
Cdd:cd18618   78 YWYVPVHHKT--NGGFAIGVAVSDSPTGPFKDALGK---PLITNDMTGTTNHSWDdIDPTvFIDDDGQAYLYWGN--PEL 150
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 218 SVRRLSAD--GLHSDPASQVQVAIGNRYEGTHVIEHDGWFYLLGSAtnccSGP-LTGYGVfagrSTSPTGPFEDRtGALL 294
Cdd:cd18618  151 YYVKLKEDmiSLDGEIGTIDISGLPDFTEAPWVHKRNGLYYLSYAA----GFPeKIAYAT----SDSPTGPWTYK-GVIM 221
                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 516970009 295 ldPRVGGTPVLHQngnrwigtghvtSAVDATGQRWMLYHAVDrtdpyVAGGGTYtKRPVLMDALDW 360
Cdd:cd18618  222 --DPAGNSFTNHP------------AIIEFKGQSYFFYHNGA-----LPGGGGF-RRSVCVDELYY 267
GH43_RcAra43A-like cd18823
Glycosyl hydrolase family 43 such as Ruminococcus champanellensis arabinanase Ara43A; This ...
94-285 2.21e-15

Glycosyl hydrolase family 43 such as Ruminococcus champanellensis arabinanase Ara43A; This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis arabinanase Ara43A and Fibrobacter succinogenes subsp. succinogenes S85 Fisuc_1994 / FSU_2517. It belongs to the GH43_CtGH43 subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43_CtGH43 includes proteins such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43) (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) which is comprised of the GH43 domain, a CBM13 domain, and a dockerin domain, exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch, and is missing an essential acidic residue suggesting a mechanism by which it bypasses beta-1,6 linked branches in the substrate. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350144 [Multi-domain]  Cd Length: 289  Bit Score: 77.00  E-value: 2.21e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  94 FSSIPTYRSADLVHWSFVGEALPTKPAWIGDGDMWA------PDVAYVG--GRYLLYYTATNTvaPGGGSAIGVATSASP 165
Cdd:cd18823   40 FKSVTLYSSTDLVNWTFEGNVLTASGAVDTAGDFAGagwvgrPGVAYNSatGKYVLLIQWGST--GNGRNGVLFATSDSP 117
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 166 AGPWVDSGgpVVEPMPSPGSADPADRRWVFDPelvtaGGEHYLYFGSYYG--GLSVRRLSADGLHSDPASQVQVAIGNRY 243
Cdd:cd18823  118 TGPFTYQR--VQPMIDNVGTNNTGDQTSFFDD-----DGKAYLVYSNDRGrgSLYIAKLRSDYLGIEPAVRIDNYVGPGR 190
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
gi 516970009 244 EGTHVIEHDGWFYLLGSATNCCSGPLTGYGVfagrSTSPTGP 285
Cdd:cd18823  191 EGNALFKYGGTYYLCASDLHGWNASQTYYMV----ATSLTGP 228
GH43_CoXyl43_like cd18619
Glycosyl hydrolase family 43 protein such as metagenomic beta-xylosidase ...
66-333 4.26e-15

Glycosyl hydrolase family 43 protein such as metagenomic beta-xylosidase/alpha-L-arabinofuranosidase CoXyl43; This glycosyl hydrolase family 43 (GH43) subgroup belongs to the GH43_AXH-like subgroup which includes enzymes that have been characterized with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55), alpha-1,2-L-arabinofuranosidase 43A (arabinan-specific; EC 3.2.1.-), endo-alpha-L-arabinanase as well as arabinoxylan arabinofuranohydrolase (AXH) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. Included in this subfamily is the metagenomic beta-xylosidase/alpha-L-arabinofuranosidase CoXyl43, which shows synergy with Trichoderma reesei cellulases and promotes plant biomass saccharification by degrading xylo-oligosaccharides, such as xylobiose and xylotriose, into the monosaccharide xylose. Studies show that the hydrolytic activity of CoXyl43 is stimulated in the presence of calcium. Several of these enzymes also contain carbohydrate binding modules (CBMs) that bind cellulose or xylan. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350131 [Multi-domain]  Cd Length: 313  Bit Score: 76.57  E-value: 4.26e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  66 DPSDPVW----YLYCTRDVIDSSLRNPDGSL-------VFSSIPTYrsADLVHwsfVGEALPTKPA-WIGDgDMWAPDVA 133
Cdd:cd18619   10 DPSAHVFdgklYIYPSHDIEADIPFNDNGDQydmkdyhVFSMDSLG--GEVVD---HGVALHVKDVpWASR-QMWAPDAA 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 134 YVGGRYLLYYTATNtvaPGGGSAIGVATSASPAGPWVdsggPVVEPMPSPGSADPAdrrwVFdpelVTAGGEHYLYFGSY 213
Cdd:cd18619   84 EKDGKYYLYFPAKD---KDGIFRIGVAVSDKPEGPFK----PEPEPIKGSYSIDPA----VF----VDDDGSYYLYFGGI 148
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 214 YGG------------------------LSVR--RLSADGLH-SDPASQVQVAIGN-----------RY-EGTHVIEHDGW 254
Cdd:cd18619  149 WGGqlqrwqtgsyvsgdgdepqddepaLGPRiaKLSPDMLSfAEPPREIVILDEDgkpllagdhdrRFfEGPWMHKYNGK 228
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 516970009 255 FYLLGSATNccsgplTGYGVFAgRSTSPTGPFedRTGALLLDPRVGGTpvlhqngnrwigTGHvtSAVDATGQRWMLYH 333
Cdd:cd18619  229 YYLSYSTGD------THLLVYA-TSDNPYGPF--TYQGVILEPVLGWT------------THH--SIVEFKGKWYLFYH 284
GH43_Pc3Gal43A-like cd18821
Glycosyl hydrolase family 43 protein such as Phanerochaete chrysosporium exo-beta-1, ...
89-290 1.24e-13

Glycosyl hydrolase family 43 protein such as Phanerochaete chrysosporium exo-beta-1,3-galactanase (Pc1, 3Gal43A, 1,3Gal43A); This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), Fusarium oxysporum 12S Fo/1 (3Gal), and Streptomyces sp. 19(2012) SGalase1 and SGalase2. It belongs to the GH43_CtGH43 subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43_CtGH43 includes proteins such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43) which is comprised of the GH43 domain, a CBM13 domain, and a dockerin domain, exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch, and is missing an essential acidic residue suggesting a mechanism by which it bypasses beta-1,6 linked branches in the substrate. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350142 [Multi-domain]  Cd Length: 262  Bit Score: 71.50  E-value: 1.24e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  89 DGSLVFSSIPTYRSADLVHWSFVGEALP------TKPAWIGdgdmWAPDVAYVG--GRYLLYYTATNtvaPGGGSA-IGV 159
Cdd:cd18821   24 DGSNLFQGVSCYSSTDLVNWTFEGLALPpqesgdLGPNRVV----ERPKVIYNPstGKYVMWMHIDS---SNYGDArVGV 96
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 160 ATSASPAGPWVDSGGPVVEPMPSpgsadpadrrW---VFdpelVTAGGEHYLYFGSYYGGLSVRRLSADGLhsDPASQVQ 236
Cdd:cd18821   97 ATSDTVTGPYTYVGSFRPLGYES----------RdigVF----QDDDGTAYLLFEDRDNGLRIYRLSDDYL--SVVELVY 160
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 516970009 237 VAIGNRYEGTHVIEHDGWFYLLGSAtnccsgpLTGYG---VFAGRSTSPTGPFEDRT 290
Cdd:cd18821  161 TFIAAGLEAPAMFKVDGTYYLLGSH-------LTGWRpndNVYFTATSLSGPWSEPG 210
GH43-like cd08986
Glycosyl hydrolase family 43 protein; uncharacterized; This glycosyl hydrolase family 43 (GH43) ...
58-289 1.46e-13

Glycosyl hydrolase family 43 protein; uncharacterized; This glycosyl hydrolase family 43 (GH43)-like subfamily includes uncharacterized enzymes similar to those with beta-1,4-xylosidase (xylan 1,4-beta-xylosidase; EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanase and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350100 [Multi-domain]  Cd Length: 257  Bit Score: 71.11  E-value: 1.46e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  58 DPDVIRSEDPsdpvWYlYCTrdvidSSLRNPDGSLVFSSIPTYRSADLVHWSFVG---------EALPTKPAWIGDGDM- 127
Cdd:cd08986    4 DPYITLGPDG----YY-YLT-----GTTGGPDWWGVNDGIRLWRSKDLKDWEYLGlvwdlekdgWWQWEPQWWTPDSKNk 73
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 128 ---WAPDVAYVGGRYLLYYTatntvAPGGGSAIGVATSASPAGPWVDSGGPVVepmpspgsadpadrRWVFDPELVTAG- 203
Cdd:cd08986   74 ralWAPEIHYINGTWYITHS-----MNGGGTGLLKSTTGKPEGPYVDPMGGPL--------------GKGIDPSLFEDDd 134
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 204 GEHYLyfgsYYGGLSVRRLSAD--GLHSDPasQVQVAIGNR---YEGTHVIEHDGWFYLLGSATNCCSGPLTGYGVFAGR 278
Cdd:cd08986  135 GTVYL----VWGNGQIARLKKDmsGFAEEP--RKIDPSGNReigHEGAFIFKIGGKYVLFGAAWSTDKMRKGTYDLYYAT 208
                        250
                 ....*....|.
gi 516970009 279 STSPTGPFEDR 289
Cdd:cd08986  209 SDSIYGPYSER 219
GH43_CtGH43-like cd18824
Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1, ...
88-285 1.89e-12

Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A; This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350145 [Multi-domain]  Cd Length: 282  Bit Score: 68.21  E-value: 1.89e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  88 PDGSLVFSSIPTYRSADLVHWSFVGEALPTKPAWIGDGDMWAPDVAY--VGGRYLLYYTATntvapGGGSAIGVATSASP 165
Cdd:cd18824   28 SCGFTLFCGFVVYSSVDLVNWTYRGVLFDPNTCAGSPGVCFRPHVVYnaRTGRYVLWYNAY-----DGSSGYAVATSSTP 102
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 166 AGPWVDsggpVVEPMPSPGSADPADRRwVFdpelVTAGGEHYLYFGS--YYGGLSVRRLSADGLHSD-PASQVQVAIGnr 242
Cdd:cd18824  103 TGPFVT----VPDPVLAPAGLQAGDFS-LF----VDDDGTGYLAYTTidFPQSIVVEQLTDDYLNTTgEYVRDLIDQE-- 171
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 516970009 243 YEGTHVIEHDGWFYLLGSATNCCSGPLTGYGVFagRSTSPTGP 285
Cdd:cd18824  172 AEAPSIFKRNGIYYILASNTCCGCCQGTGARVY--RATSPLGP 212
GH43_CtGH43-like cd08985
Glycosyl hydrolase family 43 protein such as Clostridium thermocellum exo-beta-1,3-galactanase ...
68-302 3.77e-11

Glycosyl hydrolase family 43 protein such as Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A; This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350099 [Multi-domain]  Cd Length: 273  Bit Score: 64.27  E-value: 3.77e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  68 SDPVWYLYCTrdvidsSLRNPDGSLVFSSIPTYRSADLVHWSFVGEALPTKPAWIGDGDMWA-----PDVAY--VGGRYL 140
Cdd:cd08985   11 EGDTYYWYGE------SRKGLDNDNLSHGINCYSSTDLYNWRFEGLVLPASGVEVVRDISPGyvierPKVLYnaRTRKYV 84
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 141 LY---YTATNTVApgggsAIGVATSASPAGPWVDSGGPVVEPMPSpgsadpADRRWVFDPelvtaGGEHYLYFGS-YYGG 216
Cdd:cd08985   85 MWfhlDNPNYGFA-----AVGVATSDTPTGPFTFVRSFRPDGYPS------RDMTLFQDP-----DGTAYLVRSTdHNTD 148
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 217 LSVRRLSADGLhsDPASQVQVAIGNRYEGTHVIEHDGWFYLLGS-ATNCCSGPLTGYgvfagRSTSPTGPFEDRtGALLL 295
Cdd:cd08985  149 IGISRLSDDYL--DTTGASSTFKGPKREAPALFKRGGTYYLITSgLTGWNPNPSRLA-----RADSPLGPWSTW-GNLPV 220

                 ....*..
gi 516970009 296 DPRVGGT 302
Cdd:cd08985  221 GGPGADT 227
GH43_SXA-like cd09000
Glycosyl hydrolase family 43, such as Selenomonas ruminantium beta-D-xylosidase SXA; This ...
87-367 9.42e-11

Glycosyl hydrolase family 43, such as Selenomonas ruminantium beta-D-xylosidase SXA; This glycosyl hydrolase family 43 (GH43) includes enzymes that have been characterized to mainly have beta-1,4-xylosidase (beta-D-xylosidase;xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity, including Selenomonas ruminantium (Xsa;Sxa;SXA), Bifidobacterium adolescentis ATCC 15703 (XylC;XynB;BAD_0428) and Bacillus sp. KK-1 XylB. They are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. These enzymes possess an additional C-terminal beta-sandwich domain that restricts access for substrates to a portion of the active site to form a pocket. The active-site pockets comprise of two subsites, with binding capacity for two monosaccharide moieties and a single route of access for small molecules such as substrate. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350114 [Multi-domain]  Cd Length: 292  Bit Score: 63.34  E-value: 9.42e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  87 NPDGSLV---------------FSSIPTYRSADLVHWSFVGEALPTKPA--WIGD---GDMWAPDVAYVGGRYLLYYTAT 146
Cdd:cd09000    8 NPDPSICrvgddyyiatstfewFPGVQIHHSKDLVNWELVARPLTRVSQldMRGNpdsGGIWAPCLSYADGKFWLVYTDV 87
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 147 NTV-APGGGSAIGVATSASPAGPWVDsggPVvePMPSPGsadpadrrwvFDPELV-TAGGEHYL------YFGSY--YGG 216
Cdd:cd09000   88 KSVdGPFKDVHNYLVTAESIEGPWSE---PI--YLNSSG----------FDPSLFhDDDGRKYLvnmlwdHRPGHnrFAG 152
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 217 LSVRRLSADGLHSDPASQV---QVAIGnRYEGTHVIEHDGWFYLLgsatnCCSGPlTGY--GVFAGRSTSPTGPFEdrtg 291
Cdd:cd09000  153 IVLQEFDPETKKLVGERKVifkGTELG-LTEGPHLYKRDGYYYLL-----TAEGG-TGYehAVTVARSRNIFGPYE---- 221
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 292 allLDPRvggTPVL---HQNGNRWIGTGHvTSAVDATGQRWMLYHAVDRTDPyvagggtYTKRPVL-----MDALDWQ-D 362
Cdd:cd09000  222 ---VDPD---NPLLtswDDPENPLQKAGH-GSLVETPDGEWYLAHLCGRPLP-------GRGRCPLgretaIQKVEWTdD 287

                 ....*
gi 516970009 363 GWPVV 367
Cdd:cd09000  288 GWPRL 292
GH43_62_32_68_117_130-like cd08994
Glycosyl hydrolase families: GH43, GH62, GH32, GH68, GH117, CH130; Members of the glycosyl ...
108-264 1.16e-09

Glycosyl hydrolase families: GH43, GH62, GH32, GH68, GH117, CH130; Members of the glycosyl hydrolase families 32, 43, 62, 68, 117 and 130 (GH32, GH43, GH62, GH68, GH117, GH130) all possess 5-bladed beta-propeller domains and comprise clans F and J, as classified by the carbohydrate-active enzymes database (CAZY). Clan F consists of families GH43 and GH62. GH43 includes beta-xylosidases (EC 3.2.1.37), beta-xylanases (EC 3.2.1.8), alpha-L-arabinases (EC 3.2.1.99), and alpha-L-arabinofuranosidases (EC 3.2.1.55), using aryl-glycosides as substrates, while family GH62 contains alpha-L-arabinofuranosidases (EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose sidechains from xylans. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Clan J consists of families GH32 and GH68. GH32 comprises sucrose-6-phosphate hydrolases, invertases (EC 3.2.1.26), inulinases (EC 3.2.1.7), levanases (EC 3.2.1.65), eukaryotic fructosyltransferases, and bacterial fructanotransferases while GH68 consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10); beta-fructofuranosidase (EC 3.2.1.26); inulosucrase (EC 2.4.1.9), while GH68 consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10); beta-fructofuranosidase (EC 3.2.1.26); inulosucrase (EC 2.4.1.9), all of which use sucrose as their preferential donor substrate. Members of this clan are retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) that catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. Structures of all families in the two clans manifest a funnel-shaped active site that comprises two subsites with a single route for access by ligands. Also included in this superfamily are GH117 enzymes that have exo-alpha-1,3-(3,6-anhydro)-l-galactosidase activity, removing terminal non-reducing alpha-1,3-linked 3,6-anhydro-l-galactose residues from their neoagarose substrate, and GH130 that are phosphorylases and hydrolases for beta-mannosides, involved in the bacterial utilization of mannans or N-linked glycans.


Pssm-ID: 350108 [Multi-domain]  Cd Length: 294  Bit Score: 59.97  E-value: 1.16e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 108 WSFVGEALPTKPAWIGDGDMW-APDVAYVGGRYLLYYTATNTVAPGG--------GSAIGVATSASPAGPWVDSGGPVVE 178
Cdd:cd08994   60 YKFVEVVLPGRGGGFWDGDTThNPTIKKFDGKYYLYYIGNTGPGPDPplwwghrnNQRIGVAVADSPNGPWKRFDKPILD 139
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 179 PmpspgSADPADRRWVFDPE-LVTAGGEHYLYFgsyygglsvrrlSADGLHSDPASQVQVAIGNRYEGTHVIEHDGWFYL 257
Cdd:cd08994  140 P-----RPRSWDDLITSNPAvLKRPDGSYLLYY------------KGGKKNPGGNRKHGVAVSDSPEGPYTKLSDPPVYE 202

                 ....*..
gi 516970009 258 LGSATNC 264
Cdd:cd08994  203 PGVNGQT 209
COG3940 COG3940
Beta-xylosidase, GH43 family [Carbohydrate transport and metabolism];
52-381 1.37e-09

Beta-xylosidase, GH43 family [Carbohydrate transport and metabolism];


Pssm-ID: 443140 [Multi-domain]  Cd Length: 309  Bit Score: 59.83  E-value: 1.37e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  52 LAEQCADPDVIRSEDpsdpvWYLYCtrdvidsslrnpdGSLVFSSIPTYRSADLVHWsfvGEALP----TKPA--WIGdG 125
Cdd:COG3940    3 LIEQGADPWVYKHDG-----YYYFT-------------ATVEYDRIVLRRSKTLAGL---ATAEPvvvwTPPAsgPMS-K 60
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 126 DMWAPDVAYVGGRYLLYYTATNTVAPGGGSAIGV--ATSASP-AGPWVDsggpvvepmpsPGSADPADRRWVFDPELVTA 202
Cdd:COG3940   61 NIWAPELHFIDGKWYIYFAAGDGEDNNFNHRMYVleNASADPlTGPWTE-----------KGQIKTPWDSWAIDATVFEH 129
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 203 GGEHYLYFGSYYGG------LSVRRLsadglhSDP--ASQVQVAI----------GNRY-EGTHVIEHDGWFYLLGSATN 263
Cdd:COG3940  130 NGKLYLVWSGWEGDingnqnLYIAEM------SNPwtLSGPRVLLskpeydwekiGFKVnEGPAVLKHNGKVFITYSASA 203
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 264 C-----CSGPLTGygvfagrstsptgpfedRTGALLLDPRV---GGTPVLHQN-GNRWIGTGHVTSAVDATGQR-WMLYH 333
Cdd:COG3940  204 TwdpnyCLGLLTA-----------------DAGADLLDPASwtkSPEPVFQTSpENGVYGPGHNSFTKSPDGKEdWIVYH 266
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....*....
gi 516970009 334 AVDRTDPyvaggGTYTKRPVLMDALDWQ-DGWPVVrGGAGPSDSPQPAP 381
Cdd:COG3940  267 ARSYPEI-----GCDPNRHTRAQKFTWNaDGTPDF-GTPVPDGTPLPKP 309
GH117 cd08992
Glycosyl hydrolase family 117 (GH117); This glycoside hydrolase 117 (GH117) family includes ...
102-220 1.67e-09

Glycosyl hydrolase family 117 (GH117); This glycoside hydrolase 117 (GH117) family includes alpha-1,3-L-neoagarooligosaccharide hydrolase (EC 3.2.1.-); alpha-1,3-L-neoagarobiase/neoagarobiose hydrolase (NABH, EC 3.2.1.-). In the agarolytic pathway, in order to metabolize agar, NABH is an essential enzyme because it converts alpha-neoagarobiose (O-3,6-anhydro-alpha-l-galactopyranosyl-(1,3)-d-galactose) into fermentable monosaccharides (d-galactose and 3,6-anhydro-l-galactose). Thus, these enzymes have exo-alpha-1,3-(3,6-anhydro)-l-galactosidase activity, removing terminal non-reducing alpha-1,3-linked 3,6-anhydro-l-galactose residues from their neoagarose substrate. This family includes Zobellia galactanivorans enzymes, Zg4663 and Zg3615 (also known as ZgAhgA and ZgAhgB, respectively) that have been shown to have similar activity on unsubstituted agarose oligosaccharides while Zg3597 has been shown to be inactive, possibly due to differences in dimerization conformation, active-site structure and function. GH117 shares distant sequence similarity with families GH43 and GH32. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350106  Cd Length: 314  Bit Score: 59.57  E-value: 1.67e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 102 SADLVHWSFVGEALP--TKPAWiGDGDMWAPDVAYVGGRYLLYYTATNTVAPGGGS--AIGVATSASPAGPWVDSGGPVV 177
Cdd:cd08992   72 SKDGWTWKEQGVAVGrgPKGAY-DDRSVFTPEILVHKGKYYLYYQAVKSPYGGIRDknPIGMAVADSPDGPWTKLDEPIL 150
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 516970009 178 EPMPsPGSADPADRRW----VFDPELVTAGGEHYLYF-------GSYYGGLSVR 220
Cdd:cd08992  151 EPGD-EGEWEKAKGDFdshkVHDPCLIVYNGKFYLYYkgepmgeGITGGGREIK 203
GH32-like cd18609
Glycosyl hydrolase family 32 family protein; The GH32 family contains glycosyl hydrolase ...
102-323 3.28e-08

Glycosyl hydrolase family 32 family protein; The GH32 family contains glycosyl hydrolase family GH32 proteins that cleave sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350121  Cd Length: 303  Bit Score: 55.72  E-value: 3.28e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 102 SADLVHWSFVGEALptkpaWIGDGDMWapD---------VAYVGGRYLLYYTATNTVAPGGGSAIGVATSASPAGPWVDS 172
Cdd:cd18609   49 STDLVHWERLGDAL-----GPGDPGAW--DdlatwtgsvIRDPDGLWRMFYTGTSRAEDGLVQRIGLATSDDLITWTKHP 121
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 173 GGPVVEPMPSPGSADPADRR--------WVF-DPElvtaGGEHYLYF-------GSYYGGLSVRRLSADGLH-------S 229
Cdd:cd18609  122 GNPLLAADPRWYETLGDSGWhdeawrdpWVFrDPD----GGGWHMLItaranegPPDGRGVIGHATSPDLEHwevlpplS 197
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 230 DPASQVQVaignryEGTHVIEHDGWFYLLGSATN------CCSGPLTGyGVFAGRSTSPTGPFEDRTGALLLDPRVGGTP 303
Cdd:cd18609  198 APGVFGHL------EVPQVFEIDGRWYLLFSCGAdhlsreRRAAGGGG-GTWYVPADSPLGPYDVVRARLLLPDGLYAGR 270
                        250       260
                 ....*....|....*....|
gi 516970009 304 VLHQNGNRWIGTGHVTSAVD 323
Cdd:cd18609  271 LVRDPDGRWVLLGFRNTGED 290
GH43_Bt1873-like cd08981
Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron BT_1873; This ...
66-176 1.30e-07

Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron BT_1873; This glycosyl hydrolase family 43 (GH43) subfamily includes Bacteroides thetaiotaomicron VPI-5482 endo-arabinase (Bt1873;BT_1873), as well as uncharacterized enzymes similar to those with beta-1,4-xylosidase (xylan 1,4-beta-xylosidase; EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanase and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the GH43 enzymes in this family may display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350095 [Multi-domain]  Cd Length: 289  Bit Score: 53.68  E-value: 1.30e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  66 DPSDPVWYLYCTRDViDSSLRNPDGSLVfssiptYRSADLVHWSFVGEALPTKPAWIGDGDMWAPDVAYVGGRYLLYYTA 145
Cdd:cd08981   14 DKKTGTYYLYGTTDK-DCWWGKGTGFDV------YVSKDLENWEGPYEVFRPPEDFWADRNFWAPEVHEYNGKYYLFATF 86
                         90       100       110
                 ....*....|....*....|....*....|..
gi 516970009 146 TNTVAPGGGSAIGVATsaSPAGPWVD-SGGPV 176
Cdd:cd08981   87 KAEGNGRRGTQILVSD--SPLGPFVPlSDGPV 116
GH43_62_32_68_117_130 cd08772
Glycosyl hydrolase families: GH43, GH62, GH32, GH68, GH117, CH130; Members of the glycosyl ...
101-334 2.45e-07

Glycosyl hydrolase families: GH43, GH62, GH32, GH68, GH117, CH130; Members of the glycosyl hydrolase families 32, 43, 62, 68, 117 and 130 (GH32, GH43, GH62, GH68, GH117, GH130) all possess 5-bladed beta-propeller domains and comprise clans F and J, as classified by the carbohydrate-active enzymes database (CAZY). Clan F consists of families GH43 and GH62. GH43 includes beta-xylosidases (EC 3.2.1.37), beta-xylanases (EC 3.2.1.8), alpha-L-arabinases (EC 3.2.1.99), and alpha-L-arabinofuranosidases (EC 3.2.1.55), using aryl-glycosides as substrates, while family GH62 contains alpha-L-arabinofuranosidases (EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose sidechains from xylans. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Clan J consists of families GH32 and GH68. GH32 comprises sucrose-6-phosphate hydrolases, invertases (EC 3.2.1.26), inulinases (EC 3.2.1.7), levanases (EC 3.2.1.65), eukaryotic fructosyltransferases, and bacterial fructanotransferases while GH68 consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10); beta-fructofuranosidase (EC 3.2.1.26); inulosucrase (EC 2.4.1.9), while GH68 consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10); beta-fructofuranosidase (EC 3.2.1.26); inulosucrase (EC 2.4.1.9), all of which use sucrose as their preferential donor substrate. Members of this clan are retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) that catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. Structures of all families in the two clans manifest a funnel-shaped active site that comprises two subsites with a single route for access by ligands. Also included in this superfamily are GH117 enzymes that have exo-alpha-1,3-(3,6-anhydro)-l-galactosidase activity, removing terminal non-reducing alpha-1,3-linked 3,6-anhydro-l-galactose residues from their neoagarose substrate, and GH130 that are phosphorylases and hydrolases for beta-mannosides, involved in the bacterial utilization of mannans or N-linked glycans.


Pssm-ID: 350091 [Multi-domain]  Cd Length: 257  Bit Score: 52.60  E-value: 2.45e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 101 RSADLVHWSFVGEALPTKPAWIGDGDM-WAPDVAYVGGRYLLYYTATNTVAPGG-GSAIGVATSASPAGPWVDSGGPVVE 178
Cdd:cd08772   31 RSKDLIHWEEEPPAIVARGGGSYDTSYaFDPEVVYIEGTYYLTYCSDDLGDILRhGQHIGVAYSKDPKGPWTRKDAPLIE 110
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 179 PmpspgsaDPADRRWVFDPELV--TAGGEHYLYFGSYYGGLSVRRL----SADGLHSDPASQVQ--VAIGNRYEGTHVIE 250
Cdd:cd08772  111 P-------PNAYSPKNRDPVLFprKIGKYYLLNVPSDNGHTRFGKIaiaeSPD*LHWINHSFVYnyNEQGKVGEGPSLWK 183
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 251 HDGWFYLLGSATnccsgPLTGYGvfaGRSTSPTGPFEDRTGALLLDprvggTPVLHQNGNRWIGT-GHVTSAV-DATGQR 328
Cdd:cd08772  184 TKGGWYLIYHAN-----TLTGYG---YGFGYALGDLDDPSKVLYRS-----RPEEEYETVGFKPNvVAPAAFLcDSTGIV 250

                 ....*.
gi 516970009 329 WMLYHA 334
Cdd:cd08772  251 AIIGHA 256
GH43_Bt3655-like cd08983
Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 ...
58-176 3.84e-07

Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 arabinofuranosidase Bt3655; This glycosyl hydrolase family 43 (GH43)-like family includes the characterized arabinofuranosidases (EC 3.2.1.55): Bacteroides thetaiotaomicron VPI-5482 (Bt3655;BT_3655) and Penicillium chrysogenum 31B Abf43B, as well as Bifidobacterium adolescentis ATCC 15703 beta-xylosidase (EC 3.2.1.37) BAD_1527. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350097  Cd Length: 262  Bit Score: 51.85  E-value: 3.84e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  58 DPDVIRSEDpsDPVWYLYCTrdviDSSLRNPDGSLVFSSIPTYRSADLVHWSFvgEALPTKPAWIGDGDMWAPDVAY--V 135
Cdd:cd08983   20 DPFIIRGPE--DGKFYLVAT----DLWIAGGAQWNGSRGIGVWESTDLVNWSE--QRLVKMVSPPNAGNAWAPEAIYdpE 91
                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 516970009 136 GGRYLLYYTATNTVAPGGGSA-IGVATSA-----SPAGPWVDSGGPV 176
Cdd:cd08983   92 TGQYVVYWSSSLYGDGGGGNHrIYYATTKdfktfSEPKVLFDPGFNV 138
GH32_FFase cd08996
Glycosyl hydrolase family 32, beta-fructosidases; Glycosyl hydrolase family GH32 cleaves ...
101-216 2.44e-06

Glycosyl hydrolase family 32, beta-fructosidases; Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350110  Cd Length: 281  Bit Score: 49.56  E-value: 2.44e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 101 RSADLVHWSFVGEAL-PTKPAwiGDGDMWAPDVAYVGGRYLLYYTATNTVAPGGGSaIGVATSASPAGPWVDSGGPVVEP 179
Cdd:cd08996   38 VSDDLVHWEHLPIALaPPGGY--DEDGCFSGSAVVDDGKPTLFYTGVRDLGDGRQT-QCLATSDDDLITWEKYPGNPVIP 114
                         90       100       110
                 ....*....|....*....|....*....|....*..
gi 516970009 180 MPSPGSAdpadRRWvFDPELVTAGGEHYLYFGSYYGG 216
Cdd:cd08996  115 PPPGGGV----TDF-RDPFVWKEGGTWYMVVGGGLED 146
SacC COG1621
Sucrose-6-phosphate hydrolase SacC, GH32 family [Carbohydrate transport and metabolism];
58-155 4.92e-06

Sucrose-6-phosphate hydrolase SacC, GH32 family [Carbohydrate transport and metabolism];


Pssm-ID: 441228 [Multi-domain]  Cd Length: 459  Bit Score: 49.53  E-value: 4.92e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  58 DPDVIRSEDpsdpVWYLyctrdVIDSSLRNPDGSLVFssiptYRSADLVHWSFVGEALPTKPAWigdGDMW-APDVAYVG 136
Cdd:COG1621  142 DPKVWWDDG----KWYM-----VLGAQTGDGKGTVLL-----YTSPDLKNWTYLGEFGEGDGAF---GYMWeCPDLFPLD 204
                         90
                 ....*....|....*....
gi 516970009 137 GRYLLYYTAtNTVAPGGGS 155
Cdd:COG1621  205 GKWVLIFSP-QGGGPEGGS 222
GH32_FFase cd08996
Glycosyl hydrolase family 32, beta-fructosidases; Glycosyl hydrolase family GH32 cleaves ...
58-158 5.79e-06

Glycosyl hydrolase family 32, beta-fructosidases; Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350110  Cd Length: 281  Bit Score: 48.40  E-value: 5.79e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  58 DPDVIRSEDpsdpVWYLyctrdVIDSSLRNPDGSLVFssiptYRSADLVHWSFVGEALPTKPaWIGDGDMWA-PDVAYVG 136
Cdd:cd08996  126 DPFVWKEGG----TWYM-----VVGGGLEDGGGAVLL-----YRSDDLRDWEYLGVLLDAAS-DGDTGEMWEcPDFFPLG 190
                         90       100
                 ....*....|....*....|...
gi 516970009 137 GRYLLYYTATNTVAP-GGGSAIG 158
Cdd:cd08996  191 GKWVLLFSPQGGGNLlGVVYLIG 213
GH43_LbAraf43-like cd18820
Glycosyl hydrolase family 43 proteins similar to Lactobacillus brevis ...
57-335 1.14e-05

Glycosyl hydrolase family 43 proteins similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans GbtXyl43B; This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes enzymes with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55) and possibly bifunctional xylosidase/arabinofuranosidase activities, similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans IT-08 beta-xylosidase / exo-xylanase (GbtXyl43B). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350141 [Multi-domain]  Cd Length: 258  Bit Score: 47.52  E-value: 1.14e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  57 ADPDVIRSEDpsdpvWYLYCTRDvidsslrnpdgslvFSSIPTYRSADLvhwSFVGEALP----TKPAWIGDGDMWAPDV 132
Cdd:cd18820    1 ADPWVVYHDG-----YYYLTFTT--------------GDRITIWKSKTL---TGLGTAEPkvvwTPPDPSRSCNIWAPEL 58
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 133 AYVGGRYLLYYTATNtvapGGGSA--IGVATSAS---PAGPWVDsggpvvepmpsPGSADPADRRWVFDPELVTAGGEHY 207
Cdd:cd18820   59 HFIDGRWYIYYAADD----GDNANhrMYVLESASddpPLGPYTF-----------KGRLADPTDKWAIDGTVLEHNGKLY 123
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 208 LYFGSYYGGLSVR------RLsadglhSDPASqvqvAIGNRY------------------EGTHVIEHDGWFYLLGSATN 263
Cdd:cd18820  124 FVWSGWEGDTNGPqnlyiaPM------SNPWT----LSGERVlistptydwerqgppgvnEGPQVLYHGGRTFLVYSASG 193
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 264 C-----CSGPLTGygvfagrstsptgpfedRTGALLLDP---RVGGTPVLHQNGNRWI-GTGH---VTSAvDATgQRWML 331
Cdd:cd18820  194 SwtpdyCLGLLTL-----------------TGGDDPLDPaswTKSPGPVFQSNDANGVyGPGHnsfFKSP-DGT-EDWIV 254

                 ....
gi 516970009 332 YHAV 335
Cdd:cd18820  255 YHAK 258
GH43-like cd08982
Glycosyl hydrolase family 43 protein; uncharacterized; This glycosyl hydrolase family 43 (GH43) ...
55-212 2.53e-05

Glycosyl hydrolase family 43 protein; uncharacterized; This glycosyl hydrolase family 43 (GH43)-like subfamily includes uncharacterized enzymes similar to those with beta-1,4-xylosidase (xylan 1,4-beta-xylosidase; EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanase and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350096 [Multi-domain]  Cd Length: 308  Bit Score: 46.79  E-value: 2.53e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  55 QCADPDVIRSEDpsdpVWYLYCTRdvidsslrnpdgslvfsSIPTYRSADLVHWSFVgealPTKPAWIGDgdmWAPDVAY 134
Cdd:cd08982    4 EAADPTVVLFKG----KYYLFASK-----------------SGGYWHSDDLVNWKFI----PTNGLPIED---YAPTVVE 55
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 135 VGGRylLYYTATNTVAPgggsaigVATSASPAGPWVDsggpVVEPMPSPGSADPA-----DRRWvfdpelvtaggehYLY 209
Cdd:cd08982   56 INGT--LYFTASGGPGP-------IYRTDDPLGGKWE----LVAESGPFGFWDPAlfvddDGRL-------------YLY 109

                 ...
gi 516970009 210 FGS 212
Cdd:cd08982  110 WGC 112
GH43_CtGH43-like cd18822
Glycosyl hydrolase family 43 protein such as Clostridium thermocellum exo-beta-1,3-galactanase ...
86-267 3.63e-05

Glycosyl hydrolase family 43 protein such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43); This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43), Streptomyces avermitilis MA-4680 = NBRC 14893 (Sa1,3Gal43A;SAV2109) (1,3Gal43A), and Ruminiclostridium thermocellum ATCC 27405 (Ct1,3Gal43A;CtGH43;Cthe_0661) (1,3Gal43A). It belongs to the GH43_CtGH43 subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43_CtGH43 includes proteins such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43) which is comprised of the GH43 domain, a CBM13 domain, and a dockerin domain, exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch, and is missing an essential acidic residue suggesting a mechanism by which it bypasses beta-1,6 linked branches in the substrate. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350143  Cd Length: 266  Bit Score: 46.07  E-value: 3.63e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  86 RNPDGSlvFSSIPTYRSADLVHWSFVGEALPTK---PAWIGDGDMWAPDVAY--VGGRYLLYYTATNTVAPGGGsAIGVA 160
Cdd:cd18822   22 RDNNNG--FNGVSLYSSTDLVNWEFRNTVLTRDtcsASELASCKIERPKVIYnpKTGKFVMWAHWENGKDYGLA-RAAVA 98
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 161 TSASPAGPWVDSGGpvVEPMpspG--SADPAdrrwVF-DPE----LVTAGGEHYlyfgsyygGLSVRRLSADglHSDPAS 233
Cdd:cd18822   99 TSDTPDGDYTFHGS--FRPL---GydSRDMT----LFvDDDgtayLISAANDNA--------DLNIYRLTPD--YLSVDS 159
                        170       180       190
                 ....*....|....*....|....*....|....*
gi 516970009 234 QV-QVAIGNRYEGTHVIEHDGWFYLLGSAtncCSG 267
Cdd:cd18822  160 LVaTLFKGQHREAPALVKRNGYYYLFTSG---ASG 191
GH32_EcAec43-like cd08995
Glycosyl hydrolase family 32, such as the putative glycoside hydrolase Escherichia coli Aec43 ...
69-162 3.98e-05

Glycosyl hydrolase family 32, such as the putative glycoside hydrolase Escherichia coli Aec43 (FosGH2); This glycosyl hydrolase family 32 (GH32) subgroup includes Escherichia coli strain BEN2908 putative glycoside hydrolase Aec43 (FosGH2). GH32 enzymes cleave sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). GH32 family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize.


Pssm-ID: 350109 [Multi-domain]  Cd Length: 281  Bit Score: 46.03  E-value: 3.98e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  69 DPVWYLYCTRDvidssLRNPDGSLVFSSIPTYRSADLVHWSFVGEALPTKPAWIGDGDMWAPDVAYVGGRYLLYYTATNT 148
Cdd:cd08995    9 DGKFHLFYLHD-----PRDPAPHRGGHPWALVTTKDLVHWTEHGEAIPYGGDDDQDLAIGTGSVIKDDGTYHAFYTGHNP 83
                         90
                 ....*....|....
gi 516970009 149 VAPGGGSAIGVATS 162
Cdd:cd08995   84 DFGKPKQVIMHATS 97
GH43_CtGH43-like cd18825
Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1, ...
100-285 1.03e-04

Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A; This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350146 [Multi-domain]  Cd Length: 285  Bit Score: 44.90  E-value: 1.03e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 100 YRSADLVHWSFVGEALPTKPAWIGDGDMWA-----PDVAY--VGGRYLLYYTATNTVAPGGGSAIGVATSASPAGP--WV 170
Cdd:cd18825   38 YSSKDLYNWKDEGIVLDAVDDAPASDLYPNnvverPKVIYnkKTKKYVMWFHLDGPGADYSRARAGVAVSDSPTGPfkYL 117
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 171 DSGgpvvEPMPSPGSADPADRRWVFDPEL-VTAGGEHYLYFGSYYGG-LSVRRLSADglHSDPASQ-VQVAIGNRYEGTH 247
Cdd:cd18825  118 GSF----RPNAGEKNRDFSNGQMSRDMTLfVDDDGKAYLIYSSEENKtLYIAKLTDD--YTGVTGDyARILIGQSREAPA 191
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 516970009 248 VIEHDGWFYLLGSATnccsgplTGYG---VFAGRSTSPTGP 285
Cdd:cd18825  192 VFKHDGKYYMITSGC-------TGWApnaARYAVADSIFGP 225
GH43_LbAraf43-like cd08980
Glycosyl hydrolase family 43 proteins such as Lactobacillus brevis alpha-L-arabinofuranosidase ...
57-334 2.33e-04

Glycosyl hydrolase family 43 proteins such as Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans GbtXyl43B; This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55) and possibly bifunctional xylosidase/arabinofuranosidase activities. In addition to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans IT-08 beta-xylosidase / exo-xylanase (GbtXyl43B). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) familiesGH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350094 [Multi-domain]  Cd Length: 276  Bit Score: 43.53  E-value: 2.33e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  57 ADPDVIRSEDpsdpvwYLYCTrdvidsslrnpdGSLVFSSIPTYRSADLVHWSFVGEALPTKPAWIGD--GDMWAPDVAY 134
Cdd:cd08980    1 ADPYIFKHED------YYYFT------------ATVGANRIYLRKADTLLGLSTAEEVTIWDKPPSGDysGLIWAPEIHY 62
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 135 VGGRYLLYYTATNTVAPGGG---SAIGVATSASP-AGPWVDSGGPVVEPMPSPGSADPADRR--WVFDPELVTAGGEHYL 208
Cdd:cd08980   63 IDGKWYIYFAASPENDFGAGhhrMYVLKNLSGDPfEGDWGEPGEIANNDADRQTVKDGLTNTesFSLDCTVFKVNGKLYF 142
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 209 YFGSYYGGLS--------VRRLSADGLHSDPASQVQVAIG------NRYEGTHVIEHDGWFYLLGSAT-----NCCSGPL 269
Cdd:cd08980  143 TWAQRDGDPEkfnqciyiAKMENPWTLKGDPVMLTKPEYDwekkgdKVNEGPAVVEHNGKVYLAYSASafwddNYAIGML 222
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 270 TGYGvfagrstsptgpfedrtGALLLDP---RVGGTPVLHQN-GNRWIGTGHVTSAVDATGQR-WMLYHA 334
Cdd:cd08980  223 TAEA-----------------GADLLNPaswTKSPTPVFKSSdENGQYGPGHNSFVKSEDGTEdWIVYHA 275
GH43_PcXyl-like cd18833
Glycosyl hydrolase family 43 protein such as the bifunctional Phanerochaete chrysosporium ...
92-329 3.00e-04

Glycosyl hydrolase family 43 protein such as the bifunctional Phanerochaete chrysosporium xylosidase/arabinofuranosidase (Xyl;PcXyl); This glycosyl hydrolase family 43 (GH43) subgroup includes Phanerochaete chrysosporium BKM-F-1767 Xyl, a characterized bifunctional enzyme with beta-1,4-xylosidase (beta-D-xylosidase;xylan 1,4-beta-xylosidase; EC 3.2.1.37)/ alpha-L-arabinofuranosidase (EC 3.2.1.55) activities. This subgroup belongs to the GH43_XybB subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. The GH43_XybB subgroup includes enzymes having beta-1,4-xylosidase and alpha-L-arabinofuranosidase activities. Beta-1,4-xylosidases are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43_XybB subgroup includes Bacteroides ovatus alpha-L-arabinofuranosidases, BoGH43A and BoGH43B, both having a two-domain architecture, consisting of an N-terminal 5-bladed beta-propeller domain harboring the catalytic active site, and a C-terminal beta-sandwich domain. However, despite significant functional overlap between these two enzymes, BoGH43A and BoGH43B share just 41% sequence identity. The latter appears to be significantly less active on the same substrates, suggesting that these paralogs may play subtly different roles during the degradation of xyloglucans from different sources, or may function most optimally at different stages in the catabolism of xyloglucan oligosaccharides (XyGOs), for example before or after hydrolysis of certain side-chain moieties. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350154  Cd Length: 292  Bit Score: 43.39  E-value: 3.00e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  92 LVFSSIPTYRSADLVHWSFVG------EALPTKP--AWIGDGDMWAPDVAYVGGRylLYYTATNtVAPGGGSA----IGV 159
Cdd:cd18833   31 LAFPGIPIYASKDLINWKLISnvlsrpSQLPELAttGTGQQGGIWAPTLRYHDGT--FYVITTL-VFPDKTDAsrwdNLL 107
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 160 ATSASPAGP--WVDsggPVvePMPSPGsadpadrrwvFDPELV-TAGGEHYLYFGSYYGGLSVRRLSADGLHSDPASQVQ 236
Cdd:cd18833  108 FTTTDPYSDsaWSD---PI--RFDFPG----------YDPDLFwDDDGTAYVQGAHYWRVRPEIQQQEIDLKTGESLSPS 172
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 237 VAI---GNRY-EGTHVIEHDGWFYLL----GSATNccsgpltgYGVFAGRSTSPTGPFEDRTGALLLDPRvgGTPVLHQN 308
Cdd:cd18833  173 PIWngtGGSApEGPHMYKKDGWYYLLiaegGTGLG--------HSVTIARSRSIWGPYESYPSNPVLTNA--NTSEYFQT 242
                        250       260
                 ....*....|....*....|.
gi 516970009 309 gnrwigTGHVTSAVDATGQRW 329
Cdd:cd18833  243 ------VGHADLFQDANGNWW 257
GH43_CtGH43-like cd18826
Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1, ...
100-288 9.92e-04

Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A; This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350147 [Multi-domain]  Cd Length: 269  Bit Score: 41.46  E-value: 9.92e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 100 YRSADLVHWSFVGEALPTKPAWIG-----DGDMWAPDVAY--VGGRYLLYYTatntVAPGGGSA-IGVATSASPAGPW-- 169
Cdd:cd18826   39 YSSTDLYNWEDEGLIIPPDPDDPSsplhpTRIMDRPHIIYneKTGKYVCWLK----LYPGGDVQyFGVLTADSPTGPYty 114
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 170 VDSGGPvvepmPSPGSAdpADRRWVFDPElvtagGEHYLYFGSYYGGLSVRRLSADGLhsDPASQVQV-----AIGNRYE 244
Cdd:cd18826  115 VHKFLG-----PLGMGA--GDFDLVVDPD-----GKAYLYFERVHKEVVCADLTDDYT--DVTGEYSThfpglGPPFARE 180
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*..
gi 516970009 245 GTHVIEHDGWFYLLGSATnccsgplTGY---GVFAGRSTSPTGPFED 288
Cdd:cd18826  181 APAVFKRGGKHYLLTSGT-------TGYfpnPSEVAVADSYHGPWTV 220
GH43-like cd08984
Glycosyl hydrolase family 43; This glycosyl hydrolase family 43 (GH43)-like subfamily includes ...
57-144 2.12e-03

Glycosyl hydrolase family 43; This glycosyl hydrolase family 43 (GH43)-like subfamily includes uncharacterized enzymes similar to those with beta-1,4-xylosidase (xylan 1,4-beta-xylosidase; EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanase and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350098  Cd Length: 291  Bit Score: 40.69  E-value: 2.12e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  57 ADPDVIRseDPSDPVWYLYCT--RDVIDsslrNPDGSLVF-SSIPTYRSADLVHWSFVGEALPTKPAWiGDGDMWAPDVA 133
Cdd:cd08984   11 ADPVVIW--NPATKEWWMFYTqrRATVD----GPGVAWVHgTPIGIASSKDGATWTYRGTADGLGPEP-GENTFWAPEVI 83
                         90
                 ....*....|.
gi 516970009 134 YVGGRYLLYYT 144
Cdd:cd08984   84 VDGGTYHMFVT 94
SacC COG1621
Sucrose-6-phosphate hydrolase SacC, GH32 family [Carbohydrate transport and metabolism];
100-187 2.63e-03

Sucrose-6-phosphate hydrolase SacC, GH32 family [Carbohydrate transport and metabolism];


Pssm-ID: 441228 [Multi-domain]  Cd Length: 459  Bit Score: 40.67  E-value: 2.63e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009 100 YRSADLVHWSFVGEALPtkP-AWIGDGDMWAPDVAYVGGRYLLYYTATNTVAPGGG-SAIGVATSASpAGPWV-DSGGPV 176
Cdd:COG1621   52 ATSTDLVHWEHLPIALA--PdEEYDSGGCFSGSAVVDDGNLVLFYTGNVRDGDGGRrQYQCLAYSTD-GRTFTkYEGNPV 128
                         90
                 ....*....|.
gi 516970009 177 VEPMPSPGSAD 187
Cdd:COG1621  129 IPNPPGGYTKD 139
GH_J cd08979
Glycosyl hydrolase families 32 and 68, which form the clan GH-J; This glycosyl hydrolase ...
41-169 8.91e-03

Glycosyl hydrolase families 32 and 68, which form the clan GH-J; This glycosyl hydrolase family clan J (according to carbohydrate-active enzymes database (CAZY)) includes family 32 (GH32) and 68 (GH68). GH32 enzymes include invertase (EC 3.2.1.26) and other other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). The GH68 family consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10, also known as beta-D-fructofuranosyl transferase), beta-fructofuranosidase (EC 3.2.1.26) and inulosucrase (EC 2.4.1.9). GH32 and GH68 family enzymes are retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) and catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350093 [Multi-domain]  Cd Length: 292  Bit Score: 38.71  E-value: 8.91e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  41 NPLPLDLGGGKlaEQC-ADPDVIrsEDPSDPVWYLYCTRDvidsslRNPDGSLVFssiptYRSADLVHWSFVGEALPTKP 119
Cdd:cd08979  114 FPDELPPSSGD--NQTfRDPHVV--WDKEKGHWYMVFTAR------EGANGVLGM-----YESTDLKHWKKVMKPIASNT 178
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 516970009 120 AwigDGDMWAPDVAYVGGRYLLyYTATNTVAPGGGSAIGVATSASPAGPW 169
Cdd:cd08979  179 V---TGEWECPNLVKMNGRWYL-FFGSRGSKGITSNGIHYLYAVGPSGPW 224
GH43_C2 pfam17851
Beta xylosidase C-terminal Concanavalin A-like domain; This domain is found to the C-terminus ...
409-597 9.04e-03

Beta xylosidase C-terminal Concanavalin A-like domain; This domain is found to the C-terminus of the pfam04616 domain. This domain adopts a concanavalin A-like fold.


Pssm-ID: 436093  Cd Length: 203  Bit Score: 38.02  E-value: 9.04e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  409 SDEFDGAEMDPAWTWERDPGAATR-SLS--GGALHWDTQAAdlGPDPGAAGLPLRPLPSGDYVVETRVSSTVPSEGCCqn 485
Cdd:pfam17851   1 RDDFDSPKLGLQWQWLRNPRDESWySLTerPGYLRLYGRES--LSSLFAPSLLARRQQHFSFTATTKLEFEPQKEGEE-- 76
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 516970009  486 yaqGGLVIHQDDANFVKLSVTSIWETRQTEFGKRETAEGPGYPTYGNAVG-GPVAEATVLRLsrthTATENVYTASTSVd 564
Cdd:pfam17851  77 ---AGLVVYYNEYNHYYLGVTKDEDGGRVLRLVRCDNGELTEELAEEEVPlGGEVKTVYLRV----EVDGDTYQFSYSY- 148
                         170       180       190
                  ....*....|....*....|....*....|...
gi 516970009  565 DGATWDEAGAWttpLQATPprLGLVSMAAGGFT 597
Cdd:pfam17851 149 DGKDWKTIGPE---LDASI--LSDEYAAGGGFT 176
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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