NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|517529729|ref|WP_018699937|]
View 

RNA methyltransferase [Amorphus coralli]

Protein Classification

TrmH family RNA methyltransferase( domain architecture ID 11426248)

TrmH family RNA methyltransferase catalyzes the methylation of rRNA or tRNA at specific sites in a S-adenosyl-L-methionine (SAM)-dependent manner

CATH:  3.40.1280.10
EC:  2.1.1.-
Gene Ontology:  GO:0008173|GO:0032259|GO:0003723|GO:1904047|GO:0006396
PubMed:  11763972|18029778|17338813|30457841|8265370

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 14-275 5.61e-80

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


:

Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 242.02  E-value: 5.61e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729  14 VTSAANPVVKEIRALTQRKHRAETGLFLAEGLKLVADALEADWPVRTVVLNADFETQpmAAQVAATARARGALIVKANQS 93
Cdd:COG0566    1 MTSPSNPRVKDLRKLRDKKYREEEGLFLAEGRHAVEEALKAGREIEELLVTEGALDR--LAELLALAKAAGIPVYEVSRE 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729  94 VMARLTRRENPQAVIGVFRQ-RFAGLDAVAANPG--PVWVGLEDIRDPGNLGTIVRTVDSVAGAGIVLIGDTTDPFGLEA 170
Cdd:COG0566   79 VLDKLSGTETHQGVLAVVRPpEYADLEDLLAEAGepPLLLVLDGVQDPGNLGAILRSAAAFGVDGVILPKRRADPYNPKV 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 171 VRATMGSIFHVPVVRVTR-AAFLDWRPAFPGQVIGAHLAGAVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPM 249
Cdd:COG0566  159 VRASAGALFHVPVARVTNlADALEELKEAGFWVVGTDLDGEASLYEVDLPGPVALVLGNEGKGLSRLVLEACDELVRIPM 238

                        250       260
                 ....*....|....*....|....*.
gi 517529729 250 AGRADSLNLAVSTGVMLYELGRERLS 275
Cdd:COG0566  239 AGGVESLNVAVAAAILLYEAARQRRA 264
 
Name Accession Description Interval E-value
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 14-275 5.61e-80

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 242.02  E-value: 5.61e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729  14 VTSAANPVVKEIRALTQRKHRAETGLFLAEGLKLVADALEADWPVRTVVLNADFETQpmAAQVAATARARGALIVKANQS 93
Cdd:COG0566    1 MTSPSNPRVKDLRKLRDKKYREEEGLFLAEGRHAVEEALKAGREIEELLVTEGALDR--LAELLALAKAAGIPVYEVSRE 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729  94 VMARLTRRENPQAVIGVFRQ-RFAGLDAVAANPG--PVWVGLEDIRDPGNLGTIVRTVDSVAGAGIVLIGDTTDPFGLEA 170
Cdd:COG0566   79 VLDKLSGTETHQGVLAVVRPpEYADLEDLLAEAGepPLLLVLDGVQDPGNLGAILRSAAAFGVDGVILPKRRADPYNPKV 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 171 VRATMGSIFHVPVVRVTR-AAFLDWRPAFPGQVIGAHLAGAVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPM 249
Cdd:COG0566  159 VRASAGALFHVPVARVTNlADALEELKEAGFWVVGTDLDGEASLYEVDLPGPVALVLGNEGKGLSRLVLEACDELVRIPM 238

                        250       260
                 ....*....|....*....|....*.
gi 517529729 250 AGRADSLNLAVSTGVMLYELGRERLS 275
Cdd:COG0566  239 AGGVESLNVAVAAAILLYEAARQRRA 264
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 129-269 5.55e-49

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 158.74  E-value: 5.55e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 129 WVGLEDIRDPGNLGTIVRTVDSVAGAGIVLIGDTTDPFGLEAVRATMGSIFHVPVVRVTR-AAFLDWRPAFPGQVIGAHL 207
Cdd:cd18095    2 VLVLDGVQDPGNLGTIIRTAAAFGVDGVILSKGTVDPYNPKVVRASMGALFRLPIIRVDDlEELLEELKEAGYKVVAADL 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 517529729 208 AGAVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLYEL 269
Cdd:cd18095   82 DGGSSLYDLDLPKPVALVLGNEGNGLSDELLELCDKRVTIPMSGGVESLNVAVAAAILLYEL 143
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 130-267 2.46e-37

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 128.80  E-value: 2.46e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729  130 VGLEDIRDPGNLGTIVRTVDSVAGAGIVLIG-DTTDPFGLEAVRATMGSIFHVPVVRV-TRAAFLDWRPAFPGQVIGAHL 207
Cdd:pfam00588   4 VVLDNIQDPGNLGAIIRTAAAFGADGLILVEpRSADPYNPKAIRASAGALFHLPLVVVdNLEEALKDLKEAGYAVVATSL 83

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729  208 aGAVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLY 267
Cdd:pfam00588  84 -GGKSLYEIDFPKKVALVFGNEGTGLSNEVLELCDRLVRIPMSGDVESLNVAVAAAIALY 142
rRNA_methyl_3 TIGR00186
rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases ...
 83-273 6.23e-29

rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 129290 [Multi-domain]  Cd Length: 237  Bit Score: 110.13  E-value: 6.23e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729   83 RGALIVKANQSVMARLTRRENPQAVIGVFR-QRFAGLD----AVAANPGPVWVGLEDIRDPGNLGTIVRTVDSVAGAGIV 157
Cdd:TIGR00186  41 QGINIQLVDRQKLDQLTKGGNHQGIAAKVKpILYKDLNdlykTAKSKKQPFLLILDEITDPHNLGAILRTAEAFGVDGVI 120

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729  158 LIGDTTDPFGLEAVRATMGSIFHVPVVRVTRAAFLD--------WrpafpgQVIGAHLAGAVDYRTVKTDqPILLLMGTE 229
Cdd:TIGR00186 121 LPKRRSAPLNSTVVKTSSGAVEYVPLARVTNLSRTItklkesgfW------TVGTDLDAQDTLYQVKLTK-PLALVVGNE 193

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....
gi 517529729  230 QRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLYELGRER 273
Cdd:TIGR00186 194 GEGVSRLIKENCDFLIKIPMAGKVDSLNVSVAAGILLFEIKRQR 237
PRK11181 PRK11181
23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB;
132-275 7.77e-16

23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB;


Pssm-ID: 183021 [Multi-domain]  Cd Length: 244  Bit Score: 75.00  E-value: 7.77e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 132 LEDIRDPGNLGTIVRTVDSVAGAGIVLIGDTTDPFGLEAVRATMGSIFHVPVVRVTRAA----FLD----WrpafpgqVI 203
Cdd:PRK11181 100 LDGVTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAETVPLIRVTNLArtmrMLQekniW-------IV 172

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 517529729 204 GAhlAGAVD---YRTvKTDQPILLLMGTEQRG---LTDElsaACDQLAKIPMAGRADSLNLAVSTGVMLYELGRERLS 275
Cdd:PRK11181 173 GT--AGEADhtlYQS-KLTGPLALVMGAEGEGmrrLTRE---HCDELISIPMAGSVSSLNVSVATGICLFEAVRQRSA 244
SpoU_sub_bind smart00967
RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose ...
 42-113 5.95e-06

RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose methyltransferase substrate binding domain.


Pssm-ID: 214943 [Multi-domain]  Cd Length: 70  Bit Score: 43.29  E-value: 5.95e-06
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 517529729    42 AEGLKLVADALEADWPVRTVVLNAdfETQPMAAQVAATARARGALIVKANQSVMARLTRRENPQAVIGVFRQ 113
Cdd:smart00967   1 IEGRHAVEEALKSGRRIKELYLTE--GRAERLKELLKLAKERGIKVIEVSKKELDKLSGTANHQGVVAVVKP 70
 
Name Accession Description Interval E-value
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 14-275 5.61e-80

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 242.02  E-value: 5.61e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729  14 VTSAANPVVKEIRALTQRKHRAETGLFLAEGLKLVADALEADWPVRTVVLNADFETQpmAAQVAATARARGALIVKANQS 93
Cdd:COG0566    1 MTSPSNPRVKDLRKLRDKKYREEEGLFLAEGRHAVEEALKAGREIEELLVTEGALDR--LAELLALAKAAGIPVYEVSRE 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729  94 VMARLTRRENPQAVIGVFRQ-RFAGLDAVAANPG--PVWVGLEDIRDPGNLGTIVRTVDSVAGAGIVLIGDTTDPFGLEA 170
Cdd:COG0566   79 VLDKLSGTETHQGVLAVVRPpEYADLEDLLAEAGepPLLLVLDGVQDPGNLGAILRSAAAFGVDGVILPKRRADPYNPKV 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 171 VRATMGSIFHVPVVRVTR-AAFLDWRPAFPGQVIGAHLAGAVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPM 249
Cdd:COG0566  159 VRASAGALFHVPVARVTNlADALEELKEAGFWVVGTDLDGEASLYEVDLPGPVALVLGNEGKGLSRLVLEACDELVRIPM 238

                        250       260
                 ....*....|....*....|....*.
gi 517529729 250 AGRADSLNLAVSTGVMLYELGRERLS 275
Cdd:COG0566  239 AGGVESLNVAVAAAILLYEAARQRRA 264
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 129-269 5.55e-49

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 158.74  E-value: 5.55e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 129 WVGLEDIRDPGNLGTIVRTVDSVAGAGIVLIGDTTDPFGLEAVRATMGSIFHVPVVRVTR-AAFLDWRPAFPGQVIGAHL 207
Cdd:cd18095    2 VLVLDGVQDPGNLGTIIRTAAAFGVDGVILSKGTVDPYNPKVVRASMGALFRLPIIRVDDlEELLEELKEAGYKVVAADL 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 517529729 208 AGAVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLYEL 269
Cdd:cd18095   82 DGGSSLYDLDLPKPVALVLGNEGNGLSDELLELCDKRVTIPMSGGVESLNVAVAAAILLYEL 143
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 130-267 2.46e-37

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 128.80  E-value: 2.46e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729  130 VGLEDIRDPGNLGTIVRTVDSVAGAGIVLIG-DTTDPFGLEAVRATMGSIFHVPVVRV-TRAAFLDWRPAFPGQVIGAHL 207
Cdd:pfam00588   4 VVLDNIQDPGNLGAIIRTAAAFGADGLILVEpRSADPYNPKAIRASAGALFHLPLVVVdNLEEALKDLKEAGYAVVATSL 83

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729  208 aGAVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLY 267
Cdd:pfam00588  84 -GGKSLYEIDFPKKVALVFGNEGTGLSNEVLELCDRLVRIPMSGDVESLNVAVAAAIALY 142
SpoU-like_RNA-MTase cd18109
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 130-269 5.55e-37

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349982  Cd Length: 141  Bit Score: 128.04  E-value: 5.55e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 130 VGLEDIRDPGNLGTIVRTVDSVAGAGIVLIGDTTDPFGLEAVRATMGSIFHVPVVRVTRAAFL-DWRPAFPgqVIGAHLA 208
Cdd:cd18109    3 LALDGIQDPGNLGTIIRTADWFGIRQIICSPDTVDPYNPKVVQASMGSIFRVQIHYTDLPALLaELKKAFP--VYGADLD 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 517529729 209 GAvDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGR-ADSLNLAVSTGVMLYEL 269
Cdd:cd18109   81 GE-NLYEFTLPEKGILVMGNEGHGISPEVLKLLTHRLTIPRYGKgAESLNVAVATAILLSEF 141
SpoU-like_RNA-MTase cd18104
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 132-273 8.32e-36

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349977  Cd Length: 146  Bit Score: 125.00  E-value: 8.32e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 132 LEDIRDPGNLGTIVRTVDSVAGAGIVLIGDTTDPFGLEAVRATMGSIFHVPVVRVTRAAFLDWRPAFPGQVIGAHLAGAV 211
Cdd:cd18104    5 LEGLEKPGNLGAILRSADAAGADAVILCDPKTDLFNPNVIRASLGAVFTLPVAEASSEETLAWLKENKIRILATTPKAEK 84

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 517529729 212 DYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLYELGRER 273
Cdd:cd18104   85 LYYEADLRGPIAIVVGSESEGLSEFWLKAADERVRIPMAGQADSLNVSVAAAILLYEALRQR 146
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 130-269 7.52e-34

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 120.02  E-value: 7.52e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 130 VGLEDIRDPGNLGTIVRTVDSVAGAGIVLIGDTTDPFGLEAVRATMGSIFHVPVVRV-TRAAFLDWRPAFPGQVIGAHL- 207
Cdd:cd18082    2 LVLDNIRSPGNIGSIFRTADAFGVEKIYLVGPTADIFDPKAKRTSRGTHFSVPYERVdNFEEYLKSLKEEGYPLIGLELt 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 517529729 208 AGAVDYR--TVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLYEL 269
Cdd:cd18082   82 DGSIPLSefKFKKPKKIALIFGNERSGLSPELLALCDYTVHIPMSGKNSSLNVSVATGIALYEF 145
SpoU-like_AviRb cd18107
SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes ...
 137-268 4.10e-33

SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA. AviRb is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349980  Cd Length: 148  Bit Score: 118.26  E-value: 4.10e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 137 DPGNLGTIVRTVDSVAGAGIVLIGDTTDPFGLEAVRATMGSIFHVPVVRV-TRAAFLDW----RPAFPG-QVIGAHLAGA 210
Cdd:cd18107   10 SPGNLGTIIRSADALGAHGLVVTGHAADVYDPKTIRASTGSLFSLPIVRVpSNRELEDWvearKARGTPlQLVGTDEHGD 89

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 517529729 211 VDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLYE 268
Cdd:cd18107   90 HQIADHDFTRPTLLLIGNETAGLSAAWRELCDETVSIPMAGSASSLNAACAASIFLYE 147
SpoU-like_RlmB cd18103
SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the ...
 132-269 2.88e-29

SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349976  Cd Length: 143  Bit Score: 107.86  E-value: 2.88e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 132 LEDIRDPGNLGTIVRTVDSVAGAGIVL-------IGDTtdpfgleAVRATMGSIFHVPVVRVTR-AAFLDWRPAFPGQVI 203
Cdd:cd18103    5 LDGITDPHNLGAIIRSAEAFGVDGVIIpkrrsapLTPT-------VAKASAGALEHLPIARVTNlARALEELKEAGFWIY 77

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 517529729 204 GAHLAGAVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLYEL 269
Cdd:cd18103   78 GADGEAEKSLYDVDLTGPLALVLGSEGKGLRRLVLKNCDFLVKIPMAGKVDSLNVSVAAGIILYEI 143
rRNA_methyl_3 TIGR00186
rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases ...
 83-273 6.23e-29

rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 129290 [Multi-domain]  Cd Length: 237  Bit Score: 110.13  E-value: 6.23e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729   83 RGALIVKANQSVMARLTRRENPQAVIGVFR-QRFAGLD----AVAANPGPVWVGLEDIRDPGNLGTIVRTVDSVAGAGIV 157
Cdd:TIGR00186  41 QGINIQLVDRQKLDQLTKGGNHQGIAAKVKpILYKDLNdlykTAKSKKQPFLLILDEITDPHNLGAILRTAEAFGVDGVI 120

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729  158 LIGDTTDPFGLEAVRATMGSIFHVPVVRVTRAAFLD--------WrpafpgQVIGAHLAGAVDYRTVKTDqPILLLMGTE 229
Cdd:TIGR00186 121 LPKRRSAPLNSTVVKTSSGAVEYVPLARVTNLSRTItklkesgfW------TVGTDLDAQDTLYQVKLTK-PLALVVGNE 193

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....
gi 517529729  230 QRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLYELGRER 273
Cdd:TIGR00186 194 GEGVSRLIKENCDFLIKIPMAGKVDSLNVSVAAGILLFEIKRQR 237
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 113-273 2.38e-25

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 98.31  E-value: 2.38e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 113 QRFAGLDAVAAN--PGPVWVgLEDIRDPGNLGTIVRTVDSVaGAGIVLIGDTTDPFgLEAVRATMGSIFHVPVVRV--TR 188
Cdd:cd18092    1 ERLERIEEVLARrtRDLTVV-LENVHDPHNASAVLRTCDAF-GVQDVHVIEPKNKF-KKNRGVAMGADKWLDVHRHdsTE 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 189 AAFLDWRPAfpG-QVIGAHL-AGAVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVML 266
Cdd:cd18092   78 ECLAALKEQ--GyRIVATSLsPDAVPLDELDYTRPTALVFGNEKDGVSEEALAAADERVYIPMYGFVESLNVSVAAAIIL 155


                 ....*..
gi 517529729 267 YELGRER 273
Cdd:cd18092  156 YELQRQR 162
SpoU-like cd18097
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 132-269 1.32e-19

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349970  Cd Length: 144  Bit Score: 82.61  E-value: 1.32e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 132 LEDIRDPGNLGTIVRTVDSVAGAGIVLIGDTTDPFGLEAVRATMGSIFHVP---------VVRVTRAAflDWRpafpgqV 202
Cdd:cd18097    5 LDNLRSAHNVGSIFRTADAAGVEKIYLCGYTPTPPHPKVAKTALGAEESVPwehfedtleAVRALKAR--GYT------I 76

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 517529729 203 IGAHLA-GAVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLYEL 269
Cdd:cd18097   77 IALETTeNSVPLHEARLPRPLALVLGNEVRGVSPEVLALCDAVVEIPMFGRKNSLNVAVAAAVALYEL 144
SpoU-like_MRM1 cd18105
SAM-dependent rRNA methylase related to MRM1; MRM1 catalyzes the methylation of 2'-O-ribose ...
 128-269 2.89e-19

SAM-dependent rRNA methylase related to MRM1; MRM1 catalyzes the methylation of 2'-O-ribose residues G1145 to GmG residue of the mitochondrial 16S rRNA. MRM1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349978  Cd Length: 158  Bit Score: 82.18  E-value: 2.89e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 128 VWVGLEDIRDPGNLGTIVRT-----VDsvagaGIVLIGDTTDPFGLEAVRATMGSIFHVPVVRVTR-AAFLDWRPAFPGQ 201
Cdd:cd18105    1 LWLLLDEITDPQNLGAILRSayflgVD-----GVVVSSKNSAPLSPVVSKASAGALELMPIYSVDNlASFLKSSKENGWK 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 202 VIGAHLAG----------AVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRA-----DSLNLAVSTGVML 266
Cdd:cd18105   76 VVGTSAPPsassdkktipANELSELLLEKPTILVLGNEGRGLRTNIKNLCDYLVSIPGGRSAenpgvDSLNVSVAAGILL 155


                 ...
gi 517529729 267 YEL 269
Cdd:cd18105  156 HSL 158
SpoU-like_RNMTL1 cd18106
SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) ...
 135-269 2.02e-18

SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) catalyzes the methylation of 2'-O-ribose residues G1370 to GmG residue of the mitochondrial 16S rRNA. RNMTL1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349979  Cd Length: 148  Bit Score: 79.60  E-value: 2.02e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 135 IRDPGNLGTIVRTVDSVAGAGIVLIGDTTDPFGLEAVRATMGSIFHVPVVrvtraAFLDWRPA---FPGQVIgAHLAGA- 210
Cdd:cd18106    8 IRDPGNLGSILRSAAAAGCSKVLLTKGCVDPWEPKVLRAGAGAHFRLPIY-----GNLSWDDIpnyLPDDSK-VFLADNn 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 517529729 211 ---VDYRTVKTDQ-PILLLMGTEQRGLTDE-LSAACD---QLAKIPMAGRADSLNLAVSTGVMLYEL 269
Cdd:cd18106   82 lpvQPYDSIDYCSgPIVLVIGGETHGVSPEaYKLAHStggSRVHIPLANGVDSLNSAVAASILLFEM 148
SpoU-like_NHR cd18108
Nosiheptide-resistance methyltransferase (NHR); Nosiheptide-resistance methyltransferase (NHR) ...
 130-268 9.93e-17

Nosiheptide-resistance methyltransferase (NHR); Nosiheptide-resistance methyltransferase (NHR) confers resistance to the thiazole antibiotic nosiheptide via catalyzing 2'O-methylation of 23S rRNA at the nucleotide A1067. NHR is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349981  Cd Length: 144  Bit Score: 74.82  E-value: 9.93e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 130 VGLEDIRDPGNLGTIVRTVDSVAGAGIVLI-GDTTDPFGLEAVRATMGSIFHVPVVRVTRAAFLDWRPAFPGQVIGAHLA 208
Cdd:cd18108    3 VVLDGVRIVGNIGAIVRTSLALGAAGIVLVdSDLATVADRRLLRASRGYVFSLPVVLADREEAVAFLRDNDIPLMTLDAD 82

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 517529729 209 GAVDYRTVKTD-QPILLLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLYE 268
Cdd:cd18108   83 GDVPVKELGDRpDRLALVFGSEKGGPSGLFEEAAAGTVSIPMSSSTESLNVSVSAGIALHE 143
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 138-271 6.99e-16

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 72.49  E-value: 6.99e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 138 PGNLGTIVRTvdSVA-GAGIVLIGdttdPFGLE-----AVRATMGSIFHVPVVRVTR-AAFLDWRPafPGQVIGAHLAGA 210
Cdd:cd18094   10 PQNTGNIART--CAAtGARLHLIE----PLGFSlddkrLKRAGLDYWDHVDLTVHDSwEAFLEALP--PGRLFLFTTKGT 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 517529729 211 VDYRTVKTDQPILLLMGTEQRGLTDELSAAC-DQLAKIPMAGRADSLNLAVSTGVMLYELGR 271
Cdd:cd18094   82 KPYTDFDFKPGDYLVFGRESTGLPEEILDAFpDRRLRIPMRPKVRSLNLSNSVAIVLYEALR 143
PRK11181 PRK11181
23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB;
132-275 7.77e-16

23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB;


Pssm-ID: 183021 [Multi-domain]  Cd Length: 244  Bit Score: 75.00  E-value: 7.77e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 132 LEDIRDPGNLGTIVRTVDSVAGAGIVLIGDTTDPFGLEAVRATMGSIFHVPVVRVTRAA----FLD----WrpafpgqVI 203
Cdd:PRK11181 100 LDGVTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAETVPLIRVTNLArtmrMLQekniW-------IV 172

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 517529729 204 GAhlAGAVD---YRTvKTDQPILLLMGTEQRG---LTDElsaACDQLAKIPMAGRADSLNLAVSTGVMLYELGRERLS 275
Cdd:PRK11181 173 GT--AGEADhtlYQS-KLTGPLALVMGAEGEGmrrLTRE---HCDELISIPMAGSVSSLNVSVATGICLFEAVRQRSA 244
PRK11081 PRK11081
tRNA guanosine-2'-O-methyltransferase; Provisional
 113-273 6.24e-13

tRNA guanosine-2'-O-methyltransferase; Provisional


Pssm-ID: 236837 [Multi-domain]  Cd Length: 229  Bit Score: 66.55  E-value: 6.24e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 113 QRFAGLDAVAANPGP-VWVGLEDIRDPGNLGTIVRTVDSVagagivligdttdpfGLEAVRAT-------------MGSI 178
Cdd:PRK11081   4 ERYARICEMLARRQPdLTVCMEQVHKPHNVSAIIRTADAV---------------GVHEVHAVwpgsrmrtmgstaAGSN 68

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 179 FHVPVVrvTRAAFLDWRPAFPG---QVIGAHL-AGAVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRAD 254
Cdd:PRK11081  69 SWVQVK--THRTIGDAVAHLKGqgmQILATHLsDTAVDFREIDYTRPTCILMGQEKTGISQEALALADQDIIIPMIGMVQ 146

                        170
                 ....*....|....*....
gi 517529729 255 SLNLAVSTGVMLYELGRER 273
Cdd:PRK11081 147 SLNVSVASALILYEAQRQR 165
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 138-268 1.20e-11

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 61.20  E-value: 1.20e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 138 PGNLGTIVRTvdSVA-GAGIVLIGdttdPFGLE----AV-RATMGSIFHVPVVRVTR-AAFLDWRPafPGQVI-----GA 205
Cdd:COG0219   12 PQNTGNIIRL--CAAtGARLHLIE----PLGFSlddkKLkRAGLDYWELVDLTVHDSwEAFLEALP--GGRLFlfttkGT 83

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 517529729 206 HLAGAVDYRtvKTDqpiLLLMGTEQRGLTDE-LSAACDQLAKIPMAGRADSLNLAVSTGVMLYE 268
Cdd:COG0219   84 KPYTDVDYQ--PGD---VLLFGRETAGLPEEvLAAPADQRLRIPMRPGVRSLNLSNAVAIVLYE 142
SpoU-like_TRM3-like cd18091
SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O) ...
 135-268 4.85e-10

SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O)-methyltransferase TRM3 catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in various tRNAs. TRM3 is similar to C-terminal domain of TAR (HIV-1) RNA binding protein 1 (TARBP1), a protein binding to TAR, which functions as a RNA regulatory signal by forming a stable stem-loop structure to which transactivator protein Tat binds. The role of TARBP1 is believed to be to disengage RNA polymerase II from TAR during transcriptional elongation. TRM3 and the C-terminal methyltransferase domain of TARBP1 are members of the SPOUT methyltransferase superfamily.


Pssm-ID: 349964  Cd Length: 145  Bit Score: 56.77  E-value: 4.85e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 135 IRDPGNLGTIVRTVDsVAGAGIVLIGD---TTDPfglEAVRATMGSIFHVPVVRVTRAAFLDWRPAFPGQ---VIGAHL- 207
Cdd:cd18091    8 IDKPPNLGGLCRTCE-IFGVSKLVLPNlavVEDK---QFKALSVSAEKWLPIEEVKPEDLLPYLKEKKQEgytIVGLEQt 83

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 517529729 208 AGAVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLYE 268
Cdd:cd18091   84 ANSVSLHDFQFPKKTVLVLGNEKEGIPAELLSLLDVCVEIPQLGVIRSLNVHVSGAIAIYE 144
SpoU-like cd18098
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 130-267 2.33e-09

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349971  Cd Length: 138  Bit Score: 54.58  E-value: 2.33e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 130 VGLEDIRDPGNLGTIVRTVDSVAGAGIVLIGDTTDPfgLEAVRATMGSIFHVPVVRVTraaflDWRPAFPGQVIGAHLAG 209
Cdd:cd18098    3 IGIENPKTPKNVGTLWRAAGCLGASFVFTIGARYKR--RKQSTDTSKAWRHIPLFHYD-----DLEDLAEPLPFGAVLVA 75

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 517529729 210 ------AVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGradSLNLAVSTGVMLY 267
Cdd:cd18098   76 veltegATPLPDFKHPDRAVYLLGPEDGGLPQEVLARCHHVVYLPTRG---SLNVAVAGSIVLY 136
PRK10864 PRK10864
putative methyltransferase; Provisional
 132-272 1.62e-08

putative methyltransferase; Provisional


Pssm-ID: 236779 [Multi-domain]  Cd Length: 346  Bit Score: 54.78  E-value: 1.62e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 132 LEDIRDPGNLGTIVRTVDSVAGAGIVLigdtTDPFGLE---AVRATMGSIFHVPVVRVTR--AAFLDWRPAFPGQVIGAH 206
Cdd:PRK10864 203 LEDVGNPHNLGGIMRSCAHFGVKGVVV----QDAALLEsgaAIRTAEGGAEHVQPITGDSfvDVLDDFRQAGYTIVTTSS 278

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 517529729 207 LAGAVDYRTvKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLYELGRE 272
Cdd:PRK10864 279 HKGTPLFKA-SLPAKMVLVLGQEYDGLSDAARQQGDLSVSIDGTGNVESLNVSVATGVLLAEWWRQ 343
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 219-269 2.00e-07

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 49.42  E-value: 2.00e-07
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 517529729 219 DQPILLLMGTEQRGLT-DELsAACDQLAKIPMAGRADSLNLAVSTGVMLYEL 269
Cdd:cd18093  103 GGKVALVFGREDSGLTnEEL-ALCDLLVTIPTNPEYPSLNLAQAVAIVLYEL 153
SpoU-like cd18096
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 132-268 2.01e-07

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349969  Cd Length: 140  Bit Score: 49.14  E-value: 2.01e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 517529729 132 LEDIRDPGNLGTIVRTVDSVAGAGIVLIG-DTTDPFGleavraTMGSIFHVPVVRV-TRAAFLDWRPAFPGQVIGAHLA- 208
Cdd:cd18096    5 LHNVAKRHNIGTIVRSANAFGVKEVHLVGrRKFNPFG------AMGTDRHVKFRHFdTLAEAKEYLKERGIRLVGVEIVp 78

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 517529729 209 GAVDYRTVKTDQPILLLMGTEQRGLTDELSAACDQLAKIPMAGRA-DSLNLAVSTGVMLYE 268
Cdd:cd18096   79 GAEPIETHPFPGDTAFVLGNEGTGLSDEQLAICDGFVYIPQYGSGtASLNVSVAASIVLHH 139
SpoU_sub_bind smart00967
RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose ...
 42-113 5.95e-06

RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose methyltransferase substrate binding domain.


Pssm-ID: 214943 [Multi-domain]  Cd Length: 70  Bit Score: 43.29  E-value: 5.95e-06
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 517529729    42 AEGLKLVADALEADWPVRTVVLNAdfETQPMAAQVAATARARGALIVKANQSVMARLTRRENPQAVIGVFRQ 113
Cdd:smart00967   1 IEGRHAVEEALKSGRRIKELYLTE--GRAERLKELLKLAKERGIKVIEVSKKELDKLSGTANHQGVVAVVKP 70
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 219-269 7.98e-05

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 42.80  E-value: 7.98e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 517529729 219 DQPILLLMGTEQRGLT-DELsAACDQLAKIPMAGRADSLNLAVSTGVMLYEL 269
Cdd:COG0565  105 GGKVALVFGRERSGLTnEEL-ALCDALVTIPTNPEYPSLNLAQAVQVVAYEL 155
rRNA_methyl_1 TIGR00050
RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of ...
 221-274 1.49e-04

RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases, and is now characterized, in E. coli, as a tRNA:Cm32/Um32 methyltransferase. It may be named TrMet(Xm32), or TrmJ, according to the nomenclature style chosen [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272876 [Multi-domain]  Cd Length: 233  Bit Score: 42.00  E-value: 1.49e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 517529729  221 PILLLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAVSTGVMLYELGRERL 274
Cdd:TIGR00050 105 KVALVFGREDSGLTNEELQKCHVLVSIPTSEEYPSLNLSHAVAVILYELRMAWL 158
SpoU_sub_bind pfam08032
RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose ...
 41-113 1.58e-04

RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose methyltransferase substrate binding domain.


Pssm-ID: 429793 [Multi-domain]  Cd Length: 74  Bit Score: 39.15  E-value: 1.58e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 517529729   41 LAEGLKLVADALEADWPVRTVVLNADFETqPMAAQVAATARARGALIVKANQSVMARLTRRENPQAVIGVFRQ 113
Cdd:pfam08032   1 LIYGRHAVLEALKSGPEIKKLYLTEGRRD-PRLKELLKLAKERGIKVEEVDRKKLDKLSGTANHQGVVAEVKP 72
PRK10433 PRK10433
putative RNA methyltransferase; Provisional
 224-267 4.82e-04

putative RNA methyltransferase; Provisional


Pssm-ID: 236693  Cd Length: 228  Bit Score: 40.36  E-value: 4.82e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 517529729 224 LLMGTEQRGLTDELSAACDQLAKIPMAGRADSLNLAvsTGVMLY 267
Cdd:PRK10433 110 LVFGREDSGLTNEELALADVLTGVPMAADYPSLNLG--QAVMVY 151
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH