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Conserved domains on  [gi|527036182|ref|WP_020882924|]
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MFS transporter [Enterobacter ludwigii]

Protein Classification

MFS transporter( domain architecture ID 13023745)

major facilitator superfamily (MFS) transporter facilitates the transport across cytoplasmic or internal membranes of one or more from a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides

CATH:  1.20.1250.20
Gene Ontology:  GO:0022857|GO:0055085|GO:0016020
PubMed:  23403214|26758938|26234418|26098515
SCOP:  3000310
TCDB:  2.A.1

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MFS_MelB_like cd17332
Salmonella enterica Na+/melibiose symporter MelB and similar transporters of the Major ...
 15-455 4.33e-96

Salmonella enterica Na+/melibiose symporter MelB and similar transporters of the Major Facilitator Superfamily; This family is composed of Salmonella enterica Na+/melibiose symporter MelB, Major Facilitator Superfamily domain-containing proteins, MFSD2 and MFSD12, and other sugar transporters. MelB catalyzes the electrogenic symport of galactosides with Na+, Li+ or H+. The MFSD2 subfamily is composed of two vertebrate members, MFSD2A and MFSD2B. MFSD2A is more commonly called sodium-dependent lysophosphatidylcholine symporter 1 (NLS1). It is an LPC symporter that plays an essential role for blood-brain barrier formation and function. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. MFSD2B is a potential risk or protect factor in the prognosis of lung adenocarcinoma. MelB-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


:

Pssm-ID: 340890 [Multi-domain]  Cd Length: 424  Bit Score: 297.59  E-value: 4.33e-96
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  15 LGYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNFAGTWlgrkFGRRRFFLLLS 94
Cdd:cd17332    2 IGYGLGDFGNNLIFQIVSTYLLFFYTDVLGLSAAAAGTIFLVARIIDAINDPLMGYLIDRTRSRW----GRFRPWLLWGA 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  95 SPLMFLYALLWLTDMG-----YWYYLGTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVRMICSQLGGFLAVSVPG 169
Cdd:cd17332   78 IPLALLFVLLFTTPDGsgtgkLIYALITYILLDLLYTLVNIPYTALIPELTDDPEERTSLTSWRMFFATIGGLLVTVLPP 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 170 VIMQFTGKDNPFT-YTLTGLIFSCVFCLAVFITWYTTWEAKDVHQESDFKidnqrssgvanhlKYLVLDLFSSFRIRAFR 248
Cdd:cd17332  158 PLVAYFGGGNASRgYFLTALIIGIIGIILLLICFFGTRERVVPPEEEKSK-------------LPLLKSLKALLKNRPFL 224

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 249 LHIIIYIFSFTAMDVFGSVFTYYVVYCLSQDAAAV---SGWLSIAAFASVPgtygFMLLLNRlnITPSAALRLSYGCIFA 325
Cdd:cd17332  225 ILLLAYLLYFLAFNIVNTVLVYYFKYVLGGRAELVlllLLILSGALLALLP----WPPLKKR--FGKKKAFFIGLLLAIL 298

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 326 VLVFLFTVYltktQVPTLLFSGVFILLGAARSGLYYIPWNIYSFIPDIDEIVTQQRREGIFAGVMVLTRKSTVAIAIMII 405
Cdd:cd17332  299 GLLLLFFLP----PGNLVLILVLAVLAGIGYGGANLLPWAMLADVIDYGELKTGKRREGIFYSVMTFFRKLGLALAGALV 374

                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|
gi 527036182 406 GLVLQESGFVKGSGAQPESALGAIIGLLIFATASLLAVSFFTTYKFKLTR 455
Cdd:cd17332  375 GLILSLAGYVANAAAQSASALNGIRLLIAVLPAVLLLLALILMSFYPLDK 424
 
Name Accession Description Interval E-value
MFS_MelB_like cd17332
Salmonella enterica Na+/melibiose symporter MelB and similar transporters of the Major ...
 15-455 4.33e-96

Salmonella enterica Na+/melibiose symporter MelB and similar transporters of the Major Facilitator Superfamily; This family is composed of Salmonella enterica Na+/melibiose symporter MelB, Major Facilitator Superfamily domain-containing proteins, MFSD2 and MFSD12, and other sugar transporters. MelB catalyzes the electrogenic symport of galactosides with Na+, Li+ or H+. The MFSD2 subfamily is composed of two vertebrate members, MFSD2A and MFSD2B. MFSD2A is more commonly called sodium-dependent lysophosphatidylcholine symporter 1 (NLS1). It is an LPC symporter that plays an essential role for blood-brain barrier formation and function. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. MFSD2B is a potential risk or protect factor in the prognosis of lung adenocarcinoma. MelB-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340890 [Multi-domain]  Cd Length: 424  Bit Score: 297.59  E-value: 4.33e-96
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  15 LGYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNFAGTWlgrkFGRRRFFLLLS 94
Cdd:cd17332    2 IGYGLGDFGNNLIFQIVSTYLLFFYTDVLGLSAAAAGTIFLVARIIDAINDPLMGYLIDRTRSRW----GRFRPWLLWGA 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  95 SPLMFLYALLWLTDMG-----YWYYLGTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVRMICSQLGGFLAVSVPG 169
Cdd:cd17332   78 IPLALLFVLLFTTPDGsgtgkLIYALITYILLDLLYTLVNIPYTALIPELTDDPEERTSLTSWRMFFATIGGLLVTVLPP 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 170 VIMQFTGKDNPFT-YTLTGLIFSCVFCLAVFITWYTTWEAKDVHQESDFKidnqrssgvanhlKYLVLDLFSSFRIRAFR 248
Cdd:cd17332  158 PLVAYFGGGNASRgYFLTALIIGIIGIILLLICFFGTRERVVPPEEEKSK-------------LPLLKSLKALLKNRPFL 224

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 249 LHIIIYIFSFTAMDVFGSVFTYYVVYCLSQDAAAV---SGWLSIAAFASVPgtygFMLLLNRlnITPSAALRLSYGCIFA 325
Cdd:cd17332  225 ILLLAYLLYFLAFNIVNTVLVYYFKYVLGGRAELVlllLLILSGALLALLP----WPPLKKR--FGKKKAFFIGLLLAIL 298

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 326 VLVFLFTVYltktQVPTLLFSGVFILLGAARSGLYYIPWNIYSFIPDIDEIVTQQRREGIFAGVMVLTRKSTVAIAIMII 405
Cdd:cd17332  299 GLLLLFFLP----PGNLVLILVLAVLAGIGYGGANLLPWAMLADVIDYGELKTGKRREGIFYSVMTFFRKLGLALAGALV 374

                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|
gi 527036182 406 GLVLQESGFVKGSGAQPESALGAIIGLLIFATASLLAVSFFTTYKFKLTR 455
Cdd:cd17332  375 GLILSLAGYVANAAAQSASALNGIRLLIAVLPAVLLLLALILMSFYPLDK 424
MelB COG2211
Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];
4-469 4.91e-90

Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];


Pssm-ID: 441813 [Multi-domain]  Cd Length: 447  Bit Score: 282.95  E-value: 4.91e-90
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   4 PTERRVGYGVALGYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNFagtwlGRK 83
Cdd:COG2211    2 AAKKKLSLKEKLAYGLGDLGLNLAFGLLSAYLLYFYTDVLGLSAALVGLILLVARLWDAITDPLIGALSDRT-----RTR 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  84 FGRRRFFLLLSS-PLMFLYALLWLT-----DMGYWYYLGTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVRMICS 157
Cdd:COG2211   77 WGRRRPWILIGAiPLALSFVLLFTApdlspTGKLIYALVTYLLLGLAYTLVNIPYSALGAELTPDYEERTRLSSWRFAFA 156

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 158 QLGGFLAVSVPGVIMQFTGKDNPFTYTLTGLIFSCVFCLAVFITWYTTWEAKDVHQESdfkidnqrssgvanhlKYLVLD 237
Cdd:COG2211  157 GLGGLLASVLPPPLVAAFGGDAALGYRLTALIFAVLGLLAFLLTFFGTKERPVPEEEK----------------VSLKES 220

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 238 LFSSFRIRAFRLHIIIYIFSFTAMDVFGSVFTYYVVYCLSQDAAAVSGWLSIAAFASVPGTYGFMLLLNRlnITPSAALR 317
Cdd:COG2211  221 LKALLKNRPFLLLLLAYLLFFLALALVAALLLYYFKYVLGLSAALVGLLLALYFLAALLGAPLWPRLAKR--FGKKKAFI 298

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 318 LsyGCIFAVLVFLFTVYLTKTQVPTLLFsgVFILLGAARSGLYYIPWNIYSFIPDIDEIVTQQRREGIFAGVMVLTRKST 397
Cdd:COG2211  299 I--GLLLAALGLLLLFFLGPGNLWLLLV--LAALAGIGLGAILVLPWAMLADVVDYDEWKTGRRREGLYFGIFTFAIKLG 374

                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 527036182 398 VAIAIMIIGLVLQESGFVKGSgAQPESALGAIIGLLIFATASLLAVSFFTTYKFKLTRETHKLLLKEIARRK 469
Cdd:COG2211  375 QALAGALAGLLLALFGYVAGA-AQSPSALTGIRLLFFLLPAVLLLLAALLLLFYPLTRERHAEIRAELAARR 445
MFS_2 pfam13347
MFS/sugar transport protein; This family is part of the major facilitator superfamily of ...
 16-455 1.79e-44

MFS/sugar transport protein; This family is part of the major facilitator superfamily of membrane transport proteins.


Pssm-ID: 433134 [Multi-domain]  Cd Length: 427  Bit Score: 162.09  E-value: 1.79e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   16 GYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNFAGTWlgrkfGRRRFFLLLSS 95
Cdd:pfam13347   1 GYGSGALAAGIKYAGLATYLLYFYTDVLGLSAAAVGLVLLVARLVDAFTDPIVGHIIDRTRTRW-----GRRRPWLLLSA 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   96 P-LMFLYALLWLT-DMGYW----YYLGTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVRMICSQLGGFLAVSVPG 169
Cdd:pfam13347  76 IiLAVSFILLFTPpELGRAplfiWLLATYILLRIAYTFFEIPYWSLGPELTRDYDERTSLTSYRSFFSVGGGLLAAALAF 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  170 VIMQFTG--KDNPFTYTLTGLIFSCVFCLAVFITWYTTWEakDVHQESDFKIDNQRSSGVANHLKylvldlfSSFRIRAF 247
Cdd:pfam13347 156 PLVLILGgtGLERKGYRIFALIGAVLMLLGVIITAAGTKE--RVSMRSKEDTGQKEAGSLLDMLK-------EVFRNRAF 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  248 RLHIIIYIFSFTAMDVFGSVFTYYVVYCLSQDAAAvsGWLSIAAFASVPGTYGFMLLLNRlNITPSAALRLSYgcifAVL 327
Cdd:pfam13347 227 LILLASFLLAALAMGVLNGLLLYYFRYVLGNGFAA--SAFPLVFTIGALLGIPLWPPLAK-RIGKKNTYILGA----LIT 299

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  328 VFLFTVYLTKTQVPTLLFSGVFILLGAARSGLYYIPWNIYSFIPDIDEIVTQQRREGIFAGVMVLTRKSTVAIAIMIIGL 407
Cdd:pfam13347 300 IAGFALALLLGPNNTLLFLVLYIIIGFGYGSSFFLPWSMLADVVDYGELRTGKRREGTFFAMWSFISKLATGVGLGVSGL 379

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*...
gi 527036182  408 VLQESGFVKGSGAQPESALGAIIGLLIFATASLLAVSFFTTYKFKLTR 455
Cdd:pfam13347 380 LLSAFGYNAGDSVQSPQAVTAIRLLYAVLPAVLFLVALLLLYFYPLDR 427
gph TIGR00792
sugar (Glycoside-Pentoside-Hexuronide) transporter; The Glycoside-Pentoside-Hexuronide (GPH): ...
 15-468 5.16e-32

sugar (Glycoside-Pentoside-Hexuronide) transporter; The Glycoside-Pentoside-Hexuronide (GPH):Cation Symporter Family (TC 2.A.2) GPH:cation symporters catalyze uptake of sugars in symport with a monovalent cation (H+ or Na+). Members of this family includes transporters for melibiose, lactose, raffinose, glucuronides, pentosides and isoprimeverose. Mutants of two groups of these symporters (the melibiose permeases of enteric bacteria, and the lactose permease of Streptococcus thermophilus) have been isolated in which altered cation specificity is observed or in which sugar transport is uncoupled from cation symport (i.e., uniport is catalyzed). The various members of the family can use Na+, H+ or Li, Na+ or Li+, H+ or Li+, or only H+ as the symported cation. All of these proteins possess twelve putative transmembrane a-helical spanners. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids]


Pssm-ID: 273270  Cd Length: 437  Bit Score: 127.75  E-value: 5.16e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   15 LGYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNFAGTWlgrkfGRRRFFLLLS 94
Cdd:TIGR00792   2 LSYGFGDFGNDFIFAIVSTYLLFFYTDVLGLSAAFVGTLFLVARILDAITDPIMGNIVDRTRTRW-----GKFRPWLLIG 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   95 S-PLM-FLYALLWLTDMGY-----WYYLgTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVRMICSQLGGFL-AVS 166
Cdd:TIGR00792  77 AiPFSiVLVLLFTTPDFSGtgklvYAYI-TYILLGLFYSFVNIPYWSLVPAITLDPRERESLSTFRRFGATLGGLLvAVI 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  167 VPGVIMQFTGKDNPFTYTLTGLIFSCVFCLAVFITWYTTWEakdvhQESDFKIDNQRSSGVANHLKYLvldlfssFRIRA 246
Cdd:TIGR00792 156 VLPLVSYFGGGDDKFGWFMFALVLALIGVVSLIICFFGTKE-----RYSEIPKNIEKKLSLKQIFKAL-------FKNDQ 223

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  247 FRLHIIIYIFSFTAMDVFGSVFTYYVVYCLSqDAAAVSGWLSIAAFASVPGTYGFMLLLNRLNITpsaalRLSYGCIFAV 326
Cdd:TIGR00792 224 LLILCLAYLFYNLAFNIKNGVQVYYFTYVLG-DPELFSYMGSIAIVAGLIGVLLFPRLVKKFGRK-----ILFAGGILLM 297

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  327 LVFLFTVYLTKTQVPTLLfsgVFILLGAARSGLYY-IPWNIYSFIPDIDEIVTQQRREGIFAGVMVLTRKSTVAIAIMII 405
Cdd:TIGR00792 298 VLGYLIFFFAGSNLPLIL---VLIILAGFGQNIVTgLVWALVADTVDYGEWKTGIRAEGLVYSVRTFVRKLGQALAGFFV 374

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 527036182  406 GLVLQESGFVKGSGAQPESALGAIIGLLIFATASLLAVSFFTTYKFKLTRETHKLLLKEIARR 468
Cdd:TIGR00792 375 GLILGIIGYVANAAQSPITLNGIKILMFAVPALFLLLAAIIIGRFYKLTEKKHAEIVEELEQR 437
PRK09848 PRK09848
glucuronide transporter; Provisional
 1-470 2.08e-22

glucuronide transporter; Provisional


Pssm-ID: 182109 [Multi-domain]  Cd Length: 448  Bit Score: 99.86  E-value: 2.08e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   1 MVKPTERRVgygvALGYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNFAGTWl 80
Cdd:PRK09848   1 MNQQLSWRT----IVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAAAAGTMLLLVRVFDAFADVFAGRVVDSVNTRW- 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  81 grkfGRRRFFLLLSS-PLMFLYALL------WLTDMGYWYYLGTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVR 153
Cdd:PRK09848  76 ----GKFRPFLLFGTaPLMIFSVLVfwvptdWSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAAR 151

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 154 MICSQL-GGFLAVSVPGVIMQFTGKDNPFTYTLTGLIFSCVFCLAVFITWYTTWE--AKDVHQESdFKIdnqrSSGVANH 230
Cdd:PRK09848 152 GIAASLtFVCLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTREnvVRIVAQPS-LKI----SLQTLKR 226

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 231 LKYLVLDLFSSfrirafrLHIIIYIFSFTAMDVfgsvftYYVVYCLSQdaaaVSGWLSIAAFASVPGTYGFMLLLNRLNI 310
Cdd:PRK09848 227 NRPLFMLCIGA-------LCVLISTFAVSASSL------FYVRYVLND----TGLFTVLVLVQNLVGTVASAPLVPGMVA 289

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 311 TPSAALRLSYGCIFAVLVFLFTVYLTKTQVPTLLFSGVFILLGAARSGLyyIPWNIYSFIPDIDEIVTQQRREGIFAGVM 390
Cdd:PRK09848 290 RIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQGVTMT--VMWALEADTVEYGEYLTGVRIEGLTYSLF 367

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 391 VLTRKSTVAIAIMIIGLVLQESGFVKGSGAQPESALGAIIGL-LIFATASLLAvsFFTTYKFKLTRETHKLLLKEIARRK 469
Cdd:PRK09848 368 SFTRKCGQAIGGSIPAFILGLSGYIANQVQTPEVIMGIRTSIaLVPCGFMLLA--FVIIWFYPLTDKKFKEIVVEIDNRK 445


                 .
gi 527036182 470 L 470
Cdd:PRK09848 446 K 446
 
Name Accession Description Interval E-value
MFS_MelB_like cd17332
Salmonella enterica Na+/melibiose symporter MelB and similar transporters of the Major ...
 15-455 4.33e-96

Salmonella enterica Na+/melibiose symporter MelB and similar transporters of the Major Facilitator Superfamily; This family is composed of Salmonella enterica Na+/melibiose symporter MelB, Major Facilitator Superfamily domain-containing proteins, MFSD2 and MFSD12, and other sugar transporters. MelB catalyzes the electrogenic symport of galactosides with Na+, Li+ or H+. The MFSD2 subfamily is composed of two vertebrate members, MFSD2A and MFSD2B. MFSD2A is more commonly called sodium-dependent lysophosphatidylcholine symporter 1 (NLS1). It is an LPC symporter that plays an essential role for blood-brain barrier formation and function. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. MFSD2B is a potential risk or protect factor in the prognosis of lung adenocarcinoma. MelB-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340890 [Multi-domain]  Cd Length: 424  Bit Score: 297.59  E-value: 4.33e-96
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  15 LGYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNFAGTWlgrkFGRRRFFLLLS 94
Cdd:cd17332    2 IGYGLGDFGNNLIFQIVSTYLLFFYTDVLGLSAAAAGTIFLVARIIDAINDPLMGYLIDRTRSRW----GRFRPWLLWGA 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  95 SPLMFLYALLWLTDMG-----YWYYLGTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVRMICSQLGGFLAVSVPG 169
Cdd:cd17332   78 IPLALLFVLLFTTPDGsgtgkLIYALITYILLDLLYTLVNIPYTALIPELTDDPEERTSLTSWRMFFATIGGLLVTVLPP 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 170 VIMQFTGKDNPFT-YTLTGLIFSCVFCLAVFITWYTTWEAKDVHQESDFKidnqrssgvanhlKYLVLDLFSSFRIRAFR 248
Cdd:cd17332  158 PLVAYFGGGNASRgYFLTALIIGIIGIILLLICFFGTRERVVPPEEEKSK-------------LPLLKSLKALLKNRPFL 224

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 249 LHIIIYIFSFTAMDVFGSVFTYYVVYCLSQDAAAV---SGWLSIAAFASVPgtygFMLLLNRlnITPSAALRLSYGCIFA 325
Cdd:cd17332  225 ILLLAYLLYFLAFNIVNTVLVYYFKYVLGGRAELVlllLLILSGALLALLP----WPPLKKR--FGKKKAFFIGLLLAIL 298

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 326 VLVFLFTVYltktQVPTLLFSGVFILLGAARSGLYYIPWNIYSFIPDIDEIVTQQRREGIFAGVMVLTRKSTVAIAIMII 405
Cdd:cd17332  299 GLLLLFFLP----PGNLVLILVLAVLAGIGYGGANLLPWAMLADVIDYGELKTGKRREGIFYSVMTFFRKLGLALAGALV 374

                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|
gi 527036182 406 GLVLQESGFVKGSGAQPESALGAIIGLLIFATASLLAVSFFTTYKFKLTR 455
Cdd:cd17332  375 GLILSLAGYVANAAAQSASALNGIRLLIAVLPAVLLLLALILMSFYPLDK 424
MelB COG2211
Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];
4-469 4.91e-90

Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];


Pssm-ID: 441813 [Multi-domain]  Cd Length: 447  Bit Score: 282.95  E-value: 4.91e-90
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   4 PTERRVGYGVALGYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNFagtwlGRK 83
Cdd:COG2211    2 AAKKKLSLKEKLAYGLGDLGLNLAFGLLSAYLLYFYTDVLGLSAALVGLILLVARLWDAITDPLIGALSDRT-----RTR 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  84 FGRRRFFLLLSS-PLMFLYALLWLT-----DMGYWYYLGTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVRMICS 157
Cdd:COG2211   77 WGRRRPWILIGAiPLALSFVLLFTApdlspTGKLIYALVTYLLLGLAYTLVNIPYSALGAELTPDYEERTRLSSWRFAFA 156

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 158 QLGGFLAVSVPGVIMQFTGKDNPFTYTLTGLIFSCVFCLAVFITWYTTWEAKDVHQESdfkidnqrssgvanhlKYLVLD 237
Cdd:COG2211  157 GLGGLLASVLPPPLVAAFGGDAALGYRLTALIFAVLGLLAFLLTFFGTKERPVPEEEK----------------VSLKES 220

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 238 LFSSFRIRAFRLHIIIYIFSFTAMDVFGSVFTYYVVYCLSQDAAAVSGWLSIAAFASVPGTYGFMLLLNRlnITPSAALR 317
Cdd:COG2211  221 LKALLKNRPFLLLLLAYLLFFLALALVAALLLYYFKYVLGLSAALVGLLLALYFLAALLGAPLWPRLAKR--FGKKKAFI 298

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 318 LsyGCIFAVLVFLFTVYLTKTQVPTLLFsgVFILLGAARSGLYYIPWNIYSFIPDIDEIVTQQRREGIFAGVMVLTRKST 397
Cdd:COG2211  299 I--GLLLAALGLLLLFFLGPGNLWLLLV--LAALAGIGLGAILVLPWAMLADVVDYDEWKTGRRREGLYFGIFTFAIKLG 374

                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 527036182 398 VAIAIMIIGLVLQESGFVKGSgAQPESALGAIIGLLIFATASLLAVSFFTTYKFKLTRETHKLLLKEIARRK 469
Cdd:COG2211  375 QALAGALAGLLLALFGYVAGA-AQSPSALTGIRLLFFLLPAVLLLLAALLLLFYPLTRERHAEIRAELAARR 445
MFS_2 pfam13347
MFS/sugar transport protein; This family is part of the major facilitator superfamily of ...
 16-455 1.79e-44

MFS/sugar transport protein; This family is part of the major facilitator superfamily of membrane transport proteins.


Pssm-ID: 433134 [Multi-domain]  Cd Length: 427  Bit Score: 162.09  E-value: 1.79e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   16 GYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNFAGTWlgrkfGRRRFFLLLSS 95
Cdd:pfam13347   1 GYGSGALAAGIKYAGLATYLLYFYTDVLGLSAAAVGLVLLVARLVDAFTDPIVGHIIDRTRTRW-----GRRRPWLLLSA 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   96 P-LMFLYALLWLT-DMGYW----YYLGTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVRMICSQLGGFLAVSVPG 169
Cdd:pfam13347  76 IiLAVSFILLFTPpELGRAplfiWLLATYILLRIAYTFFEIPYWSLGPELTRDYDERTSLTSYRSFFSVGGGLLAAALAF 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  170 VIMQFTG--KDNPFTYTLTGLIFSCVFCLAVFITWYTTWEakDVHQESDFKIDNQRSSGVANHLKylvldlfSSFRIRAF 247
Cdd:pfam13347 156 PLVLILGgtGLERKGYRIFALIGAVLMLLGVIITAAGTKE--RVSMRSKEDTGQKEAGSLLDMLK-------EVFRNRAF 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  248 RLHIIIYIFSFTAMDVFGSVFTYYVVYCLSQDAAAvsGWLSIAAFASVPGTYGFMLLLNRlNITPSAALRLSYgcifAVL 327
Cdd:pfam13347 227 LILLASFLLAALAMGVLNGLLLYYFRYVLGNGFAA--SAFPLVFTIGALLGIPLWPPLAK-RIGKKNTYILGA----LIT 299

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  328 VFLFTVYLTKTQVPTLLFSGVFILLGAARSGLYYIPWNIYSFIPDIDEIVTQQRREGIFAGVMVLTRKSTVAIAIMIIGL 407
Cdd:pfam13347 300 IAGFALALLLGPNNTLLFLVLYIIIGFGYGSSFFLPWSMLADVVDYGELRTGKRREGTFFAMWSFISKLATGVGLGVSGL 379

                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....*...
gi 527036182  408 VLQESGFVKGSGAQPESALGAIIGLLIFATASLLAVSFFTTYKFKLTR 455
Cdd:pfam13347 380 LLSAFGYNAGDSVQSPQAVTAIRLLYAVLPAVLFLVALLLLYFYPLDR 427
gph TIGR00792
sugar (Glycoside-Pentoside-Hexuronide) transporter; The Glycoside-Pentoside-Hexuronide (GPH): ...
 15-468 5.16e-32

sugar (Glycoside-Pentoside-Hexuronide) transporter; The Glycoside-Pentoside-Hexuronide (GPH):Cation Symporter Family (TC 2.A.2) GPH:cation symporters catalyze uptake of sugars in symport with a monovalent cation (H+ or Na+). Members of this family includes transporters for melibiose, lactose, raffinose, glucuronides, pentosides and isoprimeverose. Mutants of two groups of these symporters (the melibiose permeases of enteric bacteria, and the lactose permease of Streptococcus thermophilus) have been isolated in which altered cation specificity is observed or in which sugar transport is uncoupled from cation symport (i.e., uniport is catalyzed). The various members of the family can use Na+, H+ or Li, Na+ or Li+, H+ or Li+, or only H+ as the symported cation. All of these proteins possess twelve putative transmembrane a-helical spanners. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids]


Pssm-ID: 273270  Cd Length: 437  Bit Score: 127.75  E-value: 5.16e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   15 LGYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNFAGTWlgrkfGRRRFFLLLS 94
Cdd:TIGR00792   2 LSYGFGDFGNDFIFAIVSTYLLFFYTDVLGLSAAFVGTLFLVARILDAITDPIMGNIVDRTRTRW-----GKFRPWLLIG 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   95 S-PLM-FLYALLWLTDMGY-----WYYLgTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVRMICSQLGGFL-AVS 166
Cdd:TIGR00792  77 AiPFSiVLVLLFTTPDFSGtgklvYAYI-TYILLGLFYSFVNIPYWSLVPAITLDPRERESLSTFRRFGATLGGLLvAVI 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  167 VPGVIMQFTGKDNPFTYTLTGLIFSCVFCLAVFITWYTTWEakdvhQESDFKIDNQRSSGVANHLKYLvldlfssFRIRA 246
Cdd:TIGR00792 156 VLPLVSYFGGGDDKFGWFMFALVLALIGVVSLIICFFGTKE-----RYSEIPKNIEKKLSLKQIFKAL-------FKNDQ 223

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  247 FRLHIIIYIFSFTAMDVFGSVFTYYVVYCLSqDAAAVSGWLSIAAFASVPGTYGFMLLLNRLNITpsaalRLSYGCIFAV 326
Cdd:TIGR00792 224 LLILCLAYLFYNLAFNIKNGVQVYYFTYVLG-DPELFSYMGSIAIVAGLIGVLLFPRLVKKFGRK-----ILFAGGILLM 297

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  327 LVFLFTVYLTKTQVPTLLfsgVFILLGAARSGLYY-IPWNIYSFIPDIDEIVTQQRREGIFAGVMVLTRKSTVAIAIMII 405
Cdd:TIGR00792 298 VLGYLIFFFAGSNLPLIL---VLIILAGFGQNIVTgLVWALVADTVDYGEWKTGIRAEGLVYSVRTFVRKLGQALAGFFV 374

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 527036182  406 GLVLQESGFVKGSGAQPESALGAIIGLLIFATASLLAVSFFTTYKFKLTRETHKLLLKEIARR 468
Cdd:TIGR00792 375 GLILGIIGYVANAAQSPITLNGIKILMFAVPALFLLLAAIIIGRFYKLTEKKHAEIVEELEQR 437
PRK09848 PRK09848
glucuronide transporter; Provisional
 1-470 2.08e-22

glucuronide transporter; Provisional


Pssm-ID: 182109 [Multi-domain]  Cd Length: 448  Bit Score: 99.86  E-value: 2.08e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   1 MVKPTERRVgygvALGYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNFAGTWl 80
Cdd:PRK09848   1 MNQQLSWRT----IVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAAAAGTMLLLVRVFDAFADVFAGRVVDSVNTRW- 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  81 grkfGRRRFFLLLSS-PLMFLYALL------WLTDMGYWYYLGTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVR 153
Cdd:PRK09848  76 ----GKFRPFLLFGTaPLMIFSVLVfwvptdWSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAAR 151

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 154 MICSQL-GGFLAVSVPGVIMQFTGKDNPFTYTLTGLIFSCVFCLAVFITWYTTWE--AKDVHQESdFKIdnqrSSGVANH 230
Cdd:PRK09848 152 GIAASLtFVCLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTREnvVRIVAQPS-LKI----SLQTLKR 226

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 231 LKYLVLDLFSSfrirafrLHIIIYIFSFTAMDVfgsvftYYVVYCLSQdaaaVSGWLSIAAFASVPGTYGFMLLLNRLNI 310
Cdd:PRK09848 227 NRPLFMLCIGA-------LCVLISTFAVSASSL------FYVRYVLND----TGLFTVLVLVQNLVGTVASAPLVPGMVA 289

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 311 TPSAALRLSYGCIFAVLVFLFTVYLTKTQVPTLLFSGVFILLGAARSGLyyIPWNIYSFIPDIDEIVTQQRREGIFAGVM 390
Cdd:PRK09848 290 RIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQGVTMT--VMWALEADTVEYGEYLTGVRIEGLTYSLF 367

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 391 VLTRKSTVAIAIMIIGLVLQESGFVKGSGAQPESALGAIIGL-LIFATASLLAvsFFTTYKFKLTRETHKLLLKEIARRK 469
Cdd:PRK09848 368 SFTRKCGQAIGGSIPAFILGLSGYIANQVQTPEVIMGIRTSIaLVPCGFMLLA--FVIIWFYPLTDKKFKEIVVEIDNRK 445


                 .
gi 527036182 470 L 470
Cdd:PRK09848 446 K 446
PRK10429 PRK10429
melibiose:sodium transporter MelB;
15-459 1.20e-13

melibiose:sodium transporter MelB;


Pssm-ID: 182453  Cd Length: 473  Bit Score: 73.21  E-value: 1.20e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  15 LGYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNFAGtwlgrKFGRRRFFLLLS 94
Cdd:PRK10429   9 LSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNNTRS-----RWGKFKPWILIG 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  95 SPLMFLYALLWLTDMG------YWYYLGTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVRMICSQLGGFLAVSVP 168
Cdd:PRK10429  84 TLANSVVLFLLFSAHLfegtaqYVFVCVTYILWGMTYTIMDIPFWSLVPTLTLDKREREQLVPYPRFFASLAGFVTAGFT 163

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 169 GVIMQFTGKDNP-FTYTLTGLIFSCVFCLAVFITwyttweAKDVHQE-SDFKIDNQRSSGVAnhLKYLVLDLFSSFRIRA 246
Cdd:PRK10429 164 LPFVNYVGGGDRgFGFQMFTLVLIAFFIVSTIIT------LRNVHEVySSDNQVSAEGSHLT--LKDIVALIYKNDQLSC 235

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 247 frlhIIIYIFSF-TAMDVFGSVFTYYVVYCLSqDAAAVSGWLSIAAFASVPGTYGFMLLLNRLNITPSAALrLSYGCIFA 325
Cdd:PRK10429 236 ----LLGMALAYnIASNIINGFAIYYFTYVIG-DADLFPYYLSYAGAANLVTLILFPRLVKSLSRRILWAG-ASIFPVLS 309

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 326 VLVFLFTVYLTKTQVPTLLFSGVFILLGaarSGLYYIPWNI-YSFIPDIDEIVTQQRREGIFAGVMVLTRKSTVAIAIMI 404
Cdd:PRK10429 310 CGVLLLMGLAAPHNALLIVIAGILLNIG---TALFWVLQVImVADTVDYGEYKLGIRCESIAYSVQTMVVKGGSAFAAFF 386

                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 527036182 405 IGLVLQESGFVKGSgAQPESALGAIIGLLIFATASLLAVSFFTTYK-FKLTRETHK 459
Cdd:PRK10429 387 IGVVLGLIGYVPNV-EQSAQTLLGMQFIMIGLPTLFFMITLVLYFRyYRLNGDFLR 441
PRK09669 PRK09669
putative symporter YagG; Provisional
 15-467 1.37e-13

putative symporter YagG; Provisional


Pssm-ID: 236610  Cd Length: 444  Bit Score: 72.84  E-value: 1.37e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  15 LGYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNfagtwLGRKFGRRRFFLL-L 93
Cdd:PRK09669  12 IGYGLGDTACNLVWQTVMLFLAYFYTDVFGLSAAIMGTMFLVVRVLDAVTDPLMGALVDR-----TRTRHGQFRPYLLwF 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  94 SSPLMFLYALLWLT-DMGYW----YYLGTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVRMICSQLGGfLAVSVP 168
Cdd:PRK09669  87 AIPFGVVCLLTFYTpDFGATgkiiYACVTYILLSLVYTAINVPYCAMPGAITNDPRERHSLQSWRFALSFIGG-LIVSVI 165

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 169 GV-IMQFTGKDNP-----FTYTLTGLIFSCVFclavFITWYTTWEAkdvhqesdFKIDNQRSSGVANHLKYLVLDlfssf 242
Cdd:PRK09669 166 ALpLVDILGKGDEqkgyfYAMMVMGLLGVVLF----FCCFFMTKER--------YTPEVDNSSSVWKDLKLLLGN----- 228

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 243 riRAFRLHIIIYIFSFTAMDVFGSVFTYYVVYCLSQdAAAVSGWLSIAAFASVPGTYGFMLLLNRLNitpsaALRLSYGC 322
Cdd:PRK09669 229 --SQWRIMFIFNVVLLTAVVTRGGATLYYVNYVLLR-PDLATLFLVTGMIAGLFGALLSERLLGKFD-----RVRAFKWT 300

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 323 IFAVLVFLFTVYLTKTQVPTLLFsGVFILLGAARSGLYYIPWNIYSFIPDIDEIVTQQRREGIFAGVMVLTRKSTVAIAI 402
Cdd:PRK09669 301 IVAFVILSALIFFIPPSNVWLIF-ALNILFNFIQNLTTPLQWSMFSDVVDYEEKRSGRRLDGLVFSTNLFAIKLGLAIGG 379

                        410       420       430       440       450       460
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 527036182 403 MIIGLVLQESGFVKGSGAQPESALGAIIGLLIFATASLLAVSFFTTYKFKLTRETHKLLLKEIAR 467
Cdd:PRK09669 380 AVVGWILAWVDYVGGAAVQSASVLTTINLLFTVIPGVLFAGMAIILNFYKLNDARVEAIAQELVK 444
PRK11462 PRK11462
putative transporter; Provisional
 15-438 3.82e-11

putative transporter; Provisional


Pssm-ID: 183145  Cd Length: 460  Bit Score: 64.96  E-value: 3.82e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  15 LGYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNFAGTWlgRKFgrRRFFLLLS 94
Cdd:PRK11462  12 IGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPAGFVGTMFLVARALDAISDPCMGLLADRTRSRW--GKF--RPWVLFGA 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  95 SPL----MFLYALLWLTDMGYWYYLG-TYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVRMICSQLGGFLAVSVPG 169
Cdd:PRK11462  88 LPFgivcVLAYSTPDLSMNGKMIYAAiTYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGMLSTVLMM 167

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 170 VIMQFTGKDN-PFTYTLTGLIFSCVFCLAVFITWYTTWEAKDVHQESDFKIDnqrssgvanhlkylvlDLFSSFRIRAFR 248
Cdd:PRK11462 168 PLVNLIGGDNkPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMRE----------------DLRDIWQNDQWR 231

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 249 LHIIIYIFSFTAMDVFGSVFTYYVVYCLSqdaaavsgwlSIAAFASVPGTYGFMLLLNRLNITPSAALRLSYGCIF---- 324
Cdd:PRK11462 232 IVGLLTIFNILAVCVRGGAMMYYVTWILG----------TPEVFVAFLTTYCVGNLIGSALAKPLTDWKCKVTIFWwtna 301

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 325 AVLVFLFTVYLTKTQVPTLLFSGVFIlLGAARSGLYYIPWNIYSFIPDIDEIVTQQRREGI-FAGVMVLTrKSTVAIAIM 403
Cdd:PRK11462 302 LLAVISLAMFFVPMQASITMFVFIFV-IGVLHQLVTPIQWVMMSDTVDYGEWCNGKRLTGIsFAGTLFVL-KLGLAFGGA 379

                        410       420       430
                 ....*....|....*....|....*....|....*
gi 527036182 404 IIGLVLQESGFVKGSGAQPESALGAIIGLLIFATA 438
Cdd:PRK11462 380 LIGWMLAYGGYDAAEKAQNSATISIIIALFTIVPA 414
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 13-409 3.83e-09

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 58.59  E-value: 3.83e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  13 VALGYGITDLFGGGAFAIIGTWLLFFyttycGLSVIEAGSIFAIARVIDALLSPIMGYITDnfagtwlgrKFGRRRFfLL 92
Cdd:cd06174    1 LLLGFFLTGLARGLISPLLPALLQSF-----GLSASQLGLLFALFSLGYALLQPLAGLLAD---------RFGRRPV-LL 65

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  93 LSSPLMFLYALLWLTDMGYWYYLGTYLSIELLSAMVLVPWETLAAEMTNRyEERSRLSGVRMICSQLGGFLAVSVPGVIM 172
Cdd:cd06174   66 LGLLLFALGALLFAFAPSFWLLLLGRFLLGLGSGLIDPAVLALIADLFPE-RERGRALGLLQAFGSVGGILGPLLGGILA 144

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 173 QFTGKDNPFTYTLTGLIFSCVFCLAVFITWYTTWEAKDVHQESDFkidnqrssgvanhlKYLVLDLFSSFRIRAFRLHII 252
Cdd:cd06174  145 SSLGFGWRAVFLIAAALALLAAILLLLVVPDPPESARAKNEEASS--------------KSVLKLLKRVLKNPGLWLLLL 210

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 253 IYIFSFTAMDVFGSVFTYYVVYCLSQDAAAVSGWLSIAAFASVPGTYGFMLLLNRLNITPSAALrlsYGCIFAVLVFLFT 332
Cdd:cd06174  211 AIFLVNLAYYSFSTLLPLFLLDLGGLSVAVAGLLLSLFGLAGALGSLLLGLLSDRLIGRKPLLL---IGLLLMALGLALL 287

                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 527036182 333 VYLTKTQVPTLLFSGVFILLGaarsGLYYIPWNIYSFIpdideivTQQRREGIFAGVMVLTRKSTVAIAIMIIGLVL 409
Cdd:cd06174  288 LLAPSLLLLLLLLLLLGFGLG----GLLPLSFALIAEL-------FPPEIRGTAFGLLNTFGFLGGAIGPLLAGFLL 353
MFS_1 pfam07690
Major Facilitator Superfamily;
22-367 2.27e-07

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 52.81  E-value: 2.27e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   22 LFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDnfagtwlgrKFGRRRfFLLLSSPLMFLY 101
Cdd:pfam07690   6 FLAALGRSLLGPALPLLLAEDLGISPTEIGLLLTLFSLGYALAQPLAGRLSD---------RFGRRR-VLLIGLLLFALG 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  102 ALLWLTDMGYWYYLGTYLSIELLSAMVLVPWETLAAEMTNRyEERSRLSGVRMICSQLGGFLAVSVPGVIMQFTGKDNPF 181
Cdd:pfam07690  76 LLLLLFASSLWLLLVLRVLQGLGAGALFPAALALIADWFPP-EERGRALGLVSAGFGLGAALGPLLGGLLASLFGWRAAF 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  182 tytltglIFSCVFCLAVFITWYTTWEAKDVHQESDFKIDNQRSSGVANHLkylvldlfssFRIRAFRLHIIIYIFSFTAM 261
Cdd:pfam07690 155 -------LILAILSLLAAVLLLLPRPPPESKRPKPAEEARLSLIVAWKAL----------LRDPVLWLLLALLLFGFAFF 217

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  262 DVFGSVFTYYVVYCLSqdAAAVSGWLSIAAFASVPGTYGFMLLLNRLNITPSAALRLSYGCIFAVLVFLFTVYLTktqvp 341
Cdd:pfam07690 218 GLLTYLPLYQEVLGLS--ALLAGLLLGLGGLLGAIGRLLLGRLSDRLGRRRRLLLALLLLILAALGLLLLSLTLS----- 290

                         330       340
                  ....*....|....*....|....*.
gi 527036182  342 TLLFSGVFILLGAArSGLYYIPWNIY 367
Cdd:pfam07690 291 SLWLLLALLLLGFG-FGLVFPALNAL 315
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
22-355 1.78e-06

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 49.97  E-value: 1.78e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  22 LFGGGAFAIIGTW----LLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGyitdnfagtWLGRKFGRRRFFLLLSSPL 97
Cdd:COG2814   15 LALGAFLSGLGIGivlpALPLIAADLGASPAQAGLVVTAYLLGAALGAPLAG---------RLADRFGRRRVLLLGLLLF 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  98 MFLYALLWLTDmGYWYYLGTYLSIELLSAMVLVPWETLAAEMTNRyEERSRLSGVRMICSQLGGFLAVSVPGVIMQFTGK 177
Cdd:COG2814   86 ALGSLLCALAP-SLWLLLAARFLQGLGAGALFPAALALIADLVPP-ERRGRALGLLGAGLGLGPALGPLLGGLLADLFGW 163

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 178 DNPFtytltgLIFSCVFCLAVFITWYTTWEAKDVHQESDFKidnqrssgvanhlkylvlDLFSSFRIRAFRLHIIIYIFS 257
Cdd:COG2814  164 RWVF------LVNAVLALLALLLLLRLLPESRPAARARLRG------------------SLRELLRRPRLLLLLLLAFLL 219

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 258 FTAMDVFGSVFTYYVVYCLSQDAAAVSGWLSIAAFASVPGTYGFMLLLNRLNITPSAALrlsygcIFAVLVFLFTVYLTK 337
Cdd:COG2814  220 GFGFFALFTYLPLYLQEVLGLSASAAGLLLALFGLGGVLGALLAGRLADRFGRRRLLLI------GLLLLALGLLLLALA 293

                        330
                 ....*....|....*...
gi 527036182 338 TQVPTLLFSGVFILLGAA 355
Cdd:COG2814  294 GSLWLLLLALFLLGFGFG 311
NarK COG2223
Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];
22-214 7.29e-06

Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];


Pssm-ID: 441825 [Multi-domain]  Cd Length: 392  Bit Score: 48.34  E-value: 7.29e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  22 LFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDnfagtwlgrKFGRRRFFLLLSSPLMFLY 101
Cdd:COG2223  215 FGTFGSFIGFSSWLPPYLVDQFGLSAATAGLLAALFALLGALGRPLGGWLSD---------RIGGRRVLLIVFALMALGL 285

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 102 ALLWLTDMGYWYYLGTYLSIELLSAMVLVPWETLAAEMtNRYEERSRLSGVRMICSQLGGFLAVSVPGVIMQFTGkdnpf 181
Cdd:COG2223  286 LLLALALGSLWLFLVLFLLLGLALGGGNGAVFALVPDI-FPTKNAGAVYGLVGAAGGLGGFLGPLLFGALLDATG----- 359

                        170       180       190
                 ....*....|....*....|....*....|...
gi 527036182 182 TYTLTGLIFSCVFCLAVFITWYTTWEAKDVHQE 214
Cdd:COG2223  360 SYTAAFLVFAVLALVALVLTLLLYRRPRRAAAA 392
MelB COG2211
Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];
19-225 2.74e-05

Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];


Pssm-ID: 441813 [Multi-domain]  Cd Length: 447  Bit Score: 46.43  E-value: 2.74e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  19 ITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGyitdnfagtWLGRKFGRRRFFLLLSSPLM 98
Cdd:COG2211  235 LAYLLFFLALALVAALLLYYFKYVLGLSAALVGLLLALYFLAALLGAPLWP---------RLAKRFGKKKAFIIGLLLAA 305

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  99 FLYALLWLTDMG--YWYYLGTYLSIELLSAMVLVPWETLA-----AEMTNRYEERSRLSGVRMICSQLGGFLAVSVPGVI 171
Cdd:COG2211  306 LGLLLLFFLGPGnlWLLLVLAALAGIGLGAILVLPWAMLAdvvdyDEWKTGRRREGLYFGIFTFAIKLGQALAGALAGLL 385

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 527036182 172 MQFTGKDN--------PFTYTLTGLIFSCVFCLAVFITWYTTWEAKDVHQESDFKIDNQRSS 225
Cdd:COG2211  386 LALFGYVAgaaqspsaLTGIRLLFFLLPAVLLLLAALLLLFYPLTRERHAEIRAELAARRAA 447
MFS_MdtG_SLC18_like cd17325
bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator ...
 15-203 4.24e-05

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340883 [Multi-domain]  Cd Length: 375  Bit Score: 45.64  E-value: 4.24e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  15 LGYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDnfagtwlgrKFGRRRFFLLLS 94
Cdd:cd17325  202 LALFLAIFVLAFAFGALEPFLPLYAAELGGLSPAQIGLLFGAQGLASALSQPPAGKLSD---------RIGRKPLILIGL 272

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  95 SPLMFLYALLWLTDmGYWYYLGTYLSIELLSAMVLVPWETLAAEMTNRyEERSRLSGVRMICSQLGGFLAVSVPGVIMQF 174
Cdd:cd17325  273 LLSAVALLLLPLAT-SFWLLLLLLALLGLGLGLVFPATLALLADIVPP-EGRGTAMGLFNTAFSLGMVLGPLLGGFLYDA 350

                        170       180
                 ....*....|....*....|....*....
gi 527036182 175 TGkdnpFTYTLTGLIFSCVFCLAVFITWY 203
Cdd:cd17325  351 FG----FATPFLAAAALLLLAAVLFLLLR 375
UhpC COG2271
Sugar phosphate permease [Carbohydrate transport and metabolism];
22-198 4.96e-05

Sugar phosphate permease [Carbohydrate transport and metabolism];


Pssm-ID: 441872 [Multi-domain]  Cd Length: 363  Bit Score: 45.63  E-value: 4.96e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  22 LFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDnfagtwlgRKFGRRRFFLLLSSPLMFLY 101
Cdd:COG2271  190 FLVYFALYGFLTWLPTYLVEVRGLSLAQAGLLLSLPFLAGIVGSLLGGWLSD--------RLGRRRKLVLAIGLLLAALA 261

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 102 ALLWLTDMGYWYYLGTYLSIELLSAMVLVPWETLAAEMTNRyEERSRLSGVRMICSQLGGFLAVSVPGVIMQFTGKDNPF 181
Cdd:COG2271  262 LLLLALLPSPALAIALLFLAGFGLGGAFGLLWALAAELFPK-KARGTASGLVNTFGFLGGALGPLLVGYLLDATGYQAAF 340

                        170
                 ....*....|....*..
gi 527036182 182 TYTLTGLIFSCVFCLAV 198
Cdd:COG2271  341 LLLAALALLAALLALLL 357
MFS_MdtH_MDR_like cd17329
Multidrug resistance protein MdtH and similar multidrug resistance (MDR) transporters of the ...
 22-353 1.30e-04

Multidrug resistance protein MdtH and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily; This family is composed of Escherichia coli MdtH and similar multidrug resistance (MDR) transporters from bacteria and archaea, many of which remain uncharacterized. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. MdtH confers resistance to norfloxacin and enoxacin. MdtH-like MDR transporters belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340887 [Multi-domain]  Cd Length: 376  Bit Score: 44.14  E-value: 1.30e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  22 LFGGGAFAIIGTWLLFFYTT-----YCGLSVIEAGSIFAIARVIDALLSPIMGYITDnfagtwlgrKFGRRRFFLLLSSP 96
Cdd:cd17329    4 LLIGSFLNRLGFFMVWPFMAiylhqQLGLSASIVGLVLALSAVAGIVASLIGGRLAD---------RFGRKPVMLAGLLL 74

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  97 LMFLYALLWLTDMGYWYYLGTYLSIeLLSAMVLVPWETLAAEMTNRyEERSRLSGVRMICSQLGGFLAVSVPGVIMQftg 176
Cdd:cd17329   75 RALGFALLGFAHSPWLFAIALVLTG-FGGGLFEPASEAMIADVTTP-ENRTRAFSLLYWAINLGVAIGPLLGGLLLL--- 149

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 177 kdnpFTYTLTGLIFSCVFCLAVFITWyttweakdvhqesdFKIDNQRSSGVANHLKYLVLDLFSSFRIRAFRLHIIIYIF 256
Cdd:cd17329  150 ----YSFRLLFLAAAVLFLLAALVLL--------------FLLPETRPKRRAAEKTEILRGYKIVLRDRAFLLFLLASFL 211

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 257 SFTAMDVFGSVFTYYVVYCLSQDAAAVSGWLSIAAFASVpgtyGFMLLLNRLNITPSAALRLSYGCIFAVLVFLFTVYLt 336
Cdd:cd17329  212 ISLIYFQLESTLPVYVVEILGLGESLLGLLLALNALLIV----LLQFPVARLVERFRLRKILMIGILLFAIGFAVLAFA- 286

                        330
                 ....*....|....*..
gi 527036182 337 kTQVPTLLFSGVFILLG 353
Cdd:cd17329  287 -NTLAVLILAMIVLTLG 302
CynX COG2807
Cyanate permease [Inorganic ion transport and metabolism];
7-198 2.94e-04

Cyanate permease [Inorganic ion transport and metabolism];


Pssm-ID: 442057 [Multi-domain]  Cd Length: 399  Bit Score: 43.32  E-value: 2.94e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   7 RRVGYGVALGYGITdlfgGGAFAIIGTWLLFFYTTYcGLSVIEAGSIFAIARVIDALLSPIMGyitdnfagtWLGRKFGR 86
Cdd:COG2807  214 SPLAWLLTLFFGLQ----SLLYYAVVAWLPPILRDA-GLSAATAGLLLSLFQLAGIPGSLLVP---------LLADRLGD 279

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  87 RRFFLLLSSPLMFL-YALLWLTDMGYWYYLGTYLSIELLSAMVLVPweTLAAEMTNRYEERSRLSGVRMICSQLGGFLAV 165
Cdd:COG2807  280 RRPLLLLLGLLGLAgLLGLLLAPGSLPWLWAVLLGLGQGGLFPLAL--TLIGLRARTPAEAAALSGMAQSVGYLLAALGP 357

                        170       180       190
                 ....*....|....*....|....*....|...
gi 527036182 166 SVPGVIMQFTGkdnPFTYTLTGLIFSCVFCLAV 198
Cdd:COG2807  358 LLVGALHDATG---SWTAALLLLAALAVLMLVA 387
MFS_SLC45_SUC cd17313
Solute carrier family 45 and similar sugar transporters of the Major Facilitator Superfamily ...
 63-437 4.06e-04

Solute carrier family 45 and similar sugar transporters of the Major Facilitator Superfamily of transporters; This group includes the solute carrier 45 (SLC45) family as well as plant sucrose transporters (SUCs or SUTs) and similar proteins such as Schizosaccharomyces pombe general alpha-glucoside permease. the SLC45 family is composed of four (A1-A4) vertebrate proteins as well as related insect proteins such as Drosophila sucrose transporter SCRT or Slc45-1. Members of this group transport sucrose and other sugars like maltose into the cell, with the concomitant uptake of protons (symport system). Plant sucrose transporters are crucial to carbon partitioning, playing a key role in phloem loading/unloading. They play a key role in loading and unloading of sucrose into the phloem and as a result, they control sucrose distribution throughout the whole plant and drive the osmotic flow system in the phloem. They also play a role in the exchange of sucrose between beneficial symbionts (mycorrhiza and Rhizobium) as well as pathogens such as nematodes and parasitic fungi. There are nine sucrose transporter genes in Arabidopsis and five in rice. Vertebrate SLC45 family proteins have been implicated in the regulation of glucose homoeostasis in the brain (SLC45A1), with skin and hair pigmentation (SLC45A2), and with prostate cancer and myelination (SLC45A3). Mutations in SLC45A2, also called MATP (membrane-associated transporter protein) or melanoma antigen AIM1, cause oculocutaneous albinism type 4 (OCA4), an autosomal recessive disorder of melanin biosynthesis that results in congenital hypopigmentation of ocular and cutaneous tissues. The SLC45 family and related sugar transporters belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340871 [Multi-domain]  Cd Length: 421  Bit Score: 42.61  E-value: 4.06e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  63 LLSPIMGYITDNFAGTWLGR---KFGRRRFFLLLSSPLM--FLYALLWLTDMGYWYYLGTY--------LSIELL---SA 126
Cdd:cd17313   41 TLGPILGLFVQPLVGSLSDRcnsRIGRRRPFILVGAPLAalGLILIPNAADIGLALGDGPRtwalvlfvLGIVLLdfaMN 120

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 127 MVLVPWETLAAEMTNRyEERSRLSGVRMICSQLGGFLAVSVPGV------IMQFTGKDNPFTYTLTGLIFScvfcLAVFI 200
Cdd:cd17313  121 VCQGPVRALLPDLVPP-EQRSKANGIINFMGGLGGVLGYLLGAIlwdhnwFGFALGGNLKVPFYIGAIILL----VCVLV 195

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 201 TWYTTWEAKDVHQESDFKIDNQRSSgvanhlkyLVLDLFSSFRI--RAFRLHIIIYIFSFTAmdvFGSVFTYYVVYC--- 275
Cdd:cd17313  196 TLFFVKEPPLSPEAGEHSENPSLGE--------VLKSLLKLLKImpRSLLRLLLVIFFWWIA---FFPFELFFTDYMgee 264

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 276 -LSQDAAAVSGW--------LSIAAFASVPGTYGFMLLLNRLNITPSAALRLSYGCIFAVLVFLFtvyLTKTQVPTLLFs 346
Cdd:cd17313  265 vYHGTSAASSHYgagvrmgaWGLLIFSLAFLIFSLPIGKLGKKIGRKKVYLIGLVLFAVGMALMA---LVHNVTVALVL- 340

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 347 gvFILLGAARSGLYYIPWNIYSfipdiDEIVTQQRREGIFAGVMvltrKSTVAIAIMIIGLVLQESGFVKGSG-AQPESA 425
Cdd:cd17313  341 --FALGGIGWATININPYPLVS-----DYHSESGRGQGTDTGLL----NLAISLAQILVSLLAGPIIDVKGYGtVFPVAA 409

                        410
                 ....*....|..
gi 527036182 426 LGAIIGLLIFAT 437
Cdd:cd17313  410 VASFLSLVGALF 421
NarK COG2223
Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];
44-373 1.16e-03

Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];


Pssm-ID: 441825 [Multi-domain]  Cd Length: 392  Bit Score: 41.40  E-value: 1.16e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  44 GLSVIEAGSIFAIARVIDALLSPIMGYITDnfagtwlgrKFGRRRFFLLLSSPLMFLYALLWLTDmGYWYYLGTYLSIEL 123
Cdd:COG2223   36 GLSAAQLGLLFAIPVLVGALLRIPLGFLVD---------RFGPRRVLLIGLLLLGIGLLLLALAV-SYWLLLLLGLLLGI 105

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 124 LSAMVLVPWETLAAEMTNRyEERSRLSGVRMICSQLGGFLAVSVPGVIMQFTGkdnpftYTLTGLIFSCVFCLAVFITWY 203
Cdd:COG2223  106 GGGGSFAVGIALVSKWFPP-DRRGLALGLAAGGGNLGAAVAAFLAPLLIAAFG------WRNAFLILGILLLVVAVLAWL 178

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 204 TTWEAKDvhqesdfkidnqrssGVANHLKYLVLDLFSSFRIRAFRLHIIIYIFSFTAMDVFGSVFTYYVVYCLSQDAAAV 283
Cdd:COG2223  179 FLRDPPA---------------GAAAAAKASLRDQLEALRDPRFWLLSLLYFGTFGSFIGFSSWLPPYLVDQFGLSAATA 243

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 284 SGWLSIAAFASVPGTYGFMLLLNRLNITPSAAlrlsygcIFAVLVFLFTVYLTKTQVPTLLFSGVFILLGAArsglyYIP 363
Cdd:COG2223  244 GLLAALFALLGALGRPLGGWLSDRIGGRRVLL-------IVFALMALGLLLLALALGSLWLFLVLFLLLGLA-----LGG 311

                        330
                 ....*....|..
gi 527036182 364 WN--IYSFIPDI 373
Cdd:COG2223  312 GNgaVFALVPDI 323
MFS_MFSD12 cd17491
Major facilitator superfamily domain-containing protein 12; Major facilitator superfamily ...
 8-100 1.40e-03

Major facilitator superfamily domain-containing protein 12; Major facilitator superfamily domain-containing protein 12 (MFSD12) protein subfamily includes a group of uncharacterized proteins similar to human MFSD2. MFSD2 is composed of two vertebrate members, MFSD2A and MFSD2B. MFSD2A is an LPC symporter that plays an essential role for blood-brain barrier formation and function. MFSD2B is a potential risk or protect factor in the prognosis of lung adenocarcinoma. The MFSD12 subfamily belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341044  Cd Length: 438  Bit Score: 41.09  E-value: 1.40e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   8 RVGYGValGYGITDLFGGGAFaiigTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDNFAGTWlgrKFGRR 87
Cdd:cd17491    1 RIAYGV--GHVLNDLCASMWF----TYLLVYFHLVLGFSSALAGILLLIGQVADAISTPLVGYESDRTNGCG---KYGRR 71

                         90
                 ....*....|...
gi 527036182  88 RFFLLLSSPLMFL 100
Cdd:cd17491   72 KSWHLIGTICVLL 84
MFS_NLS1_MFSD2A cd17451
Sodium-dependent lysophosphatidylcholine symporter 1 of the Major Facilitator Superfamily of ...
 24-172 1.44e-03

Sodium-dependent lysophosphatidylcholine symporter 1 of the Major Facilitator Superfamily of transporters; Sodium-dependent lysophosphatidylcholine (LPC) symporter 1 (NLS1) is also called major facilitator superfamily domain-containing protein 2A (MFSD2A). NLS1/MFSD2A is an LPC symporter that plays an essential role for blood-brain barrier formation and function. It also transports the essential omega-3 fatty acid docosahexaenoic acid (DHA), which is essential for normal brain growth and cognitive function, in the form of LPC into the brain across the blood-brain barrier. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. NLS1/MFSD2A belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341009  Cd Length: 419  Bit Score: 40.95  E-value: 1.44e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  24 GGGAFAIIGTWLLFFYTTY----CGLSVIEAGSIFAIARVIDALLSPIMGYITDNFAGTwlgrKFGRRRFFLLLSSPLMF 99
Cdd:cd17451    7 GGAPYQITGCALGFFLQIYlldvAQLDPFYASIILFVGRAWDAITDPTVGFFVSKSPWT----RFGRLMPWIIFSTPFAV 82

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 527036182 100 L-YALLWLT-----DMGYWYYLgTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVRMICSQLGGFLAVSVPGVIM 172
Cdd:cd17451   83 LsYFLIWFVpdfsqGKVMWYLL-FYCLFQTLQTCFHVPYSALTMFISTEQKERDSATAYRMTVEVLGTVLGTAIQGQIV 160
MFS_MFSD2B cd17452
Major facilitator superfamily domain-containing protein 2B; Major facilitator superfamily ...
 24-443 1.86e-03

Major facilitator superfamily domain-containing protein 2B; Major facilitator superfamily domain-containing protein 2B (MFSD2B) is closely related to MFSD2A, and their conserved genomic structure suggests that they are derived from the duplication of an ancestral gene. Variations of chromosome 2 gene expressions among patients with lung cancer or non-cancer identified MFSD2B as a potential risk or protect factor in the prognosis of lung adenocarcinoma. MFSD2B belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341010  Cd Length: 416  Bit Score: 40.57  E-value: 1.86e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  24 GGGAFAIIGTWLLFFYTTY----CGLSVIEAGSIFAIARVIDALLSPIMGYITDNfaGTWlgRKFGRRRFFLLLSSPLMF 99
Cdd:cd17452    7 GGAPNQVAGSATAFFLQIYlldiAQITPFQASLVLFVGKAWGAATDPVVGFFISK--SKW--TKIGRLMPWMLGCTPFIV 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 100 L-YALLW----LTDMGYWYYLGTYLSIELLSAMVLVPWETLAAEMTNRYEERSRLSGVRMICSQLGGFLAVSVPGVIMQf 174
Cdd:cd17452   83 VsYFFLWfvppFTTGRFLWYLTFYCLFQALATCFHVPYSALTMFLSTDQRERDSATAYRMTVEVLGTLVGAAVQGQIVA- 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 175 tgkdnpFTYTLTGLIFSCVFCLAVFITWYTTWEAKDVHQ-ESDFKIDNQRSSG-VANHLKYLVLdlfssfrirafrlhII 252
Cdd:cd17452  162 ------SAYMIAAGVIGGLYLLCISVLFLGVKERDDPYApKSGKAIPFFKGLSlTMRHGPYLKL--------------TA 221

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 253 IYIFSFTAMDVFGSVFTYYVVYC--LSQDAAAVSGWLSIAAFASVPGTYGFMLLLNRlnitPSAALRLSYGCIFAVLVfl 330
Cdd:cd17452  222 SFLFISAAVQLEQSNFVLFCTHAvdLHDHFQNLVLTILVSAVVSIPFWQWFLQRFGK----KAAACGISWMIPFAIML-- 295

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 331 ftvyltkTQVPTLLFSGVF-ILLGAARSGLYYIPWniySFIPDI-DEIVTQQRR----EGIFAGVMVLTRKSTVAIAIMI 404
Cdd:cd17452  296 -------VTIPNLIVAYVVaFVSGLSIAASLLLPW---SMLPDVvDDFRLQNPHgkglETIFYSSYVFFTKLSAGIALGI 365

                        410       420       430       440
                 ....*....|....*....|....*....|....*....|....*.
gi 527036182 405 IGLVLQESGFVKGSGAQPESA-------LGAIIGLLIFATASLLAV 443
Cdd:cd17452  366 STLSLEFAGYETGACKQPESVvltlkllIGAAPTCMILIGLCILMF 411
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
15-164 2.09e-03

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 40.34  E-value: 2.09e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  15 LGYGITDLFGGGAFAIIGTWLLFFYTTYCGLSVIEAGSIFAIARVIDALLSPIMGYITDnfagtwlgrKFGRRRfFLLLS 94
Cdd:COG2814  210 LLLLLLAFLLGFGFFALFTYLPLYLQEVLGLSASAAGLLLALFGLGGVLGALLAGRLAD---------RFGRRR-LLLIG 279

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  95 SPLMFLYALLWLTDMGYWYYLGTYLSIELLSAMVLVPWETLAAEMTNRyEERSRLSGVRMICSQLGGFLA 164
Cdd:COG2814  280 LLLLALGLLLLALAGSLWLLLLALFLLGFGFGLLFPLLQALVAELAPP-EARGRASGLYNSAFFLGGPLA 348
MFS_MdtG_SLC18_like cd17325
bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator ...
 44-265 2.28e-03

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340883 [Multi-domain]  Cd Length: 375  Bit Score: 40.25  E-value: 2.28e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182  44 GLSVIEAGSIFAIARVIDALLSPIMGYITDnfagtwlgrKFGRRRF-----FLLLSSPLMFLYAllwltdMGYWYYLGTY 118
Cdd:cd17325   27 GASVAQIGLIVAAYALAQLLFSPPAGRLSD---------RFGRKRLlllglLLLAVSTLLFAFA------TSYWQLLLAR 91

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182 119 LSIELLSAMVLVPWETLAAEMTNRyEERSRLSGVRMICSQLGGFLAVSVPGVIMQFTGKDNPFtytltgLIFSCVFCLAV 198
Cdd:cd17325   92 FLQGLASAAVWPAAMALIADIAPP-EERGRAMGIFSSAIGLGFLIGPLLGGLLADALGYRAPF------LVCAALALLAL 164

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 527036182 199 FITWYTTWEAKDVHQESDFKIDNQRSSGVANHLKYLVLDLFSSFRIRAFRLHIIIYIFSFTAMDVFG 265
Cdd:cd17325  165 VLALLLLPEPRPPPNKRAVSAARLRSGLRLLLRDRRLLALFLAIFVLAFAFGALEPFLPLYAAELGG 231
MFS_1 pfam07690
Major Facilitator Superfamily;
22-164 2.68e-03

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 40.09  E-value: 2.68e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 527036182   22 LFGGGAFAIIGTWLLFFYTTYcGLSVIEAGSIFAIARVIDALLSPIMGYITDnfagtwlgrKFGRRRFFLLLSSPLMFLY 101
Cdd:pfam07690 210 LLFGFAFFGLLTYLPLYQEVL-GLSALLAGLLLGLGGLLGAIGRLLLGRLSD---------RLGRRRRLLLALLLLILAA 279

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 527036182  102 ALLWLTDM--GYWYYLGTYLSIELLSAMVLVPWETLAAEMTNRyEERSRLSGVRMICSQLGGFLA 164
Cdd:pfam07690 280 LGLLLLSLtlSSLWLLLALLLLGFGFGLVFPALNALVSDLAPK-EERGTASGLYNTAGSLGGALG 343
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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