|
Name |
Accession |
Description |
Interval |
E-value |
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
7-592 |
0e+00 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 830.51 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 7 HFVNRFRLQAKKWLNRTALRFREQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAV 86
Cdd:COG1022 12 TLPDLLRRRAARFPDRVALREKEDGIWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAV 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 87 AVPIYATNTAKQVEYIVNDADIKILFVGDQEQFDQVYQIANNCPQLMKIVAMKAnMHLRDLPNACYWEDFLNV---VPNE 163
Cdd:COG1022 92 TVPIYPTSSAEEVAYILNDSGAKVLFVEDQEQLDKLLEVRDELPSLRHIVVLDP-RGLRDDPRLLSLDELLALgreVADP 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 164 AEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGA 243
Cdd:COG1022 171 AELEARRAAVKPDDLATIIYTSGTTGRPKGVMLTHRNLLSNARALLERLPLGPGDRTLSFLPLAHVFERTVSYYALAAGA 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 244 TNCYLEDTKHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVGEQH----YQIEQPSQWLRLQY 319
Cdd:COG1022 251 TVAFAESPDTLAEDLREVKPTFMLAVPRVWEKVYAGIQAKAEEAGGLKRKLFRWALAVGRRYararLAGKSPSLLLRLKH 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 320 ALADKLVLTKLRALLGGRIKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVK 399
Cdd:COG1022 331 ALADKLVFSKLREALGGRLRFAVSGGAALGPELARFFRALGIPVLEGYGLTETSPVITVNRPGDNRIGTVGPPLPGVEVK 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 400 IGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDK 479
Cdd:COG1022 411 IAEDGEILVRGPNVMKGYYKNPEATAEAFDADGWLHTGDIGELDEDGFLRITGRKKDLIVTSGGKNVAPQPIENALKASP 490
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 480 FIEQIAVIADAKKYVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFERRIHELQKELPSFEQVKRFTLLPQAF 559
Cdd:COG1022 491 LIEQAVVVGDGRPFLAALIVPDFEALGEWAEENGLPYTSYAELAQDPEVRALIQEEVDRANAGLSRAEQIKRFRLLPKEF 570
|
570 580 590
....*....|....*....|....*....|...
gi 544685990 560 STAMEEITPTLKLRRKVIMQRYREQIEEMYNER 592
Cdd:COG1022 571 TIENGELTPTLKLKRKVILEKYADLIEALYAGA 603
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
32-577 |
0e+00 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 583.02 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 32 QWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKIL 111
Cdd:cd05907 2 VWQPITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKAL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 112 FVGDqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqLSDLFTLIYTSGTTGEP 191
Cdd:cd05907 82 FVED-----------------------------------------------------------PDDLATIIYTSGTTGRP 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 192 KGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFE-RAWIAYVFHRGATNCYLEDTKHVRDALTTLKPTVMCAVP 270
Cdd:cd05907 103 KGVMLSHRNILSNALALAERLPATEGDRHLSFLPLAHVFErRAGLYVPLLAGARIYFASSAETLLDDLSEVRPTVFLAVP 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 RFYEKIYTAVwdKVEKAPLHRRALFNWAIrvgeqhyqieqpsqwlrlqyaladklvltklrallGGRIKMMPCGGAKLEA 350
Cdd:cd05907 183 RVWEKVYAAI--KVKAVPGLKRKLFDLAV-----------------------------------GGRLRFAASGGAPLPA 225
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 351 SIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTE 430
Cdd:cd05907 226 ELLHFFRALGIPVYEGYGLTETSAVVTLNPPGDNRIGTVGKPLPGVEVRIADDGEILVRGPNVMLGYYKNPEATAEALDA 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 431 DGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEEYAK 510
Cdd:cd05907 306 DGWLHTGDLGEIDEDGFLHITGRKKDLIITSGGKNISPEPIENALKASPLISQAVVIGDGRPFLVALIVPDPEALEAWAE 385
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990 511 QLNIKYQDRIELIKHSDIIQMFERRIHELQKELPSFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVI 577
Cdd:cd05907 386 EHGIAYTDVAELAANPAVRAEIEAAVEAANARLSRYEQIKKFLLLPEPFTIENGELTPTLKLKRPVI 452
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
34-589 |
3.97e-134 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 402.36 E-value: 3.97e-134
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 34 QEMSWQTFQQ---EIDRFSYALIAQHIDI--QDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADI 108
Cdd:cd05927 1 GPYEWISYKEvaeRADNIGSALRSLGGKPapASFVGIYSINRPEWIISELACYAYSLVTVPLYDTLGPEAIEYILNHAEI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 109 KILFVGDQeqfdqvyqianncpqlMKIVAMKANMHLrdlpnacYWEDFLNVVPNEAEfekrlnskqlsDLFTLIYTSGTT 188
Cdd:cd05927 81 SIVFCDAG----------------VKVYSLEEFEKL-------GKKNKVPPPPPKPE-----------DLATICYTSGTT 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 189 GEPKGVMLDYANLAHQLNAHDLALNV----NEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLE-DTKHVRDALTTLKP 263
Cdd:cd05927 127 GNPKGVMLTHGNIVSNVAGVFKILEIlnkiNPTDVYISYLPLAHIFERVVEALFLYHGAKIGFYSgDIRLLLDDIKALKP 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 264 TVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVGEQHYQ--IEQPSQWLrlqyalaDKLVLTKLRALLGGRIKMM 341
Cdd:cd05927 207 TVFPGVPRVLNRIYDKIFNKVQAKGPLKRKLFNFALNYKLAELRsgVVRASPFW-------DKLVFNKIKQALGGNVRLM 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 342 PCGGAKLEASIGSFFHSI-GINIKLGYGMTETTA--TVSCWQDKgfNPNSIGTLMPNAEVKIGE-------------ENE 405
Cdd:cd05927 280 LTGSAPLSPEVLEFLRVAlGCPVLEGYGQTECTAgaTLTLPGDT--SVGHVGGPLPCAEVKLVDvpemnydakdpnpRGE 357
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 406 ILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIA 485
Cdd:cd05927 358 VCIRGPNVFSGYYKDPEKTAEALDEDGWLHTGDIGEWLPNGTLKIIDRKKNIFKLSQGEYVAPEKIENIYARSPFVAQIF 437
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 486 VIADAKK-YVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFERRIHELQKE--LPSFEQVKRFTLLPQAFSTA 562
Cdd:cd05927 438 VYGDSLKsFLVAIVVPDPDVLKEWAASKGGGTGSFEELCKNPEVKKAILEDLVRLGKEngLKGFEQVKAIHLEPEPFSVE 517
|
570 580
....*....|....*....|....*..
gi 544685990 563 MEEITPTLKLRRKVIMQRYREQIEEMY 589
Cdd:cd05927 518 NGLLTPTFKLKRPQLKKYYKKQIDEMY 544
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
25-581 |
1.01e-116 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 358.27 E-value: 1.01e-116
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 25 LRFREQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVN 104
Cdd:cd17641 1 LREKDFGIWQEFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLN 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 105 DADIKILFVGDQEQFDQVYQIANNCPQLMKIVAM-KANMHLRDLPNACYWEDFLNVVPNEA-----EFEKRLNSKQLSDL 178
Cdd:cd17641 81 YTGARVVIAEDEEQVDKLLEIADRIPSVRYVIYCdPRGMRKYDDPRLISFEDVVALGRALDrrdpgLYEREVAAGKGEDV 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 179 FTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWI--AYVFHRGATNCYLEDTKHVRD 256
Cdd:cd17641 161 AVLCTTSGTTGKPKLAMLSHGNFLGHCAAYLAADPLGPGDEYVSVLPLPWIGEQMYSvgQALVCGFIVNFPEEPETMMED 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 257 aLTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVG----EQHYQIEQPSQWLRLQYALADKLVLTKLRA 332
Cdd:cd17641 241 -LREIGPTFVLLPPRVWEGIAADVRARMMDATPFKRFMFELGMKLGlralDRGKRGRPVSLWLRLASWLADALLFRPLRD 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 333 LLG-GRIKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKIGEENEILVRGG 411
Cdd:cd17641 320 RLGfSRLRSAATGGAALGPDTFRFFHAIGVPLKQLYGQTELAGAYTVHRDGDVDPDTVGVPFPGTEVRIDEVGEILVRSP 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 412 MVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAK 491
Cdd:cd17641 400 GVFVGYYKNPEATAEDFDEDGWLHTGDAGYFKENGHLVVIDRAKDVGTTSDGTRFSPQFIENKLKFSPYIAEAVVLGAGR 479
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 492 KYVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFERRIHELQKELPSFEQVKRFTLLPQAFSTAMEEITPTLK 571
Cdd:cd17641 480 PYLTAFICIDYAIVGKWAEQRGIAFTTYTDLASRPEVYELIRKEVEKVNASLPEAQRIRRFLLLYKELDADDGELTRTRK 559
|
570
....*....|
gi 544685990 572 LRRKVIMQRY 581
Cdd:cd17641 560 VRRGVIAEKY 569
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
37-577 |
2.78e-108 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 333.17 E-value: 2.78e-108
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 37 SWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQ 116
Cdd:cd17640 7 TYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVALVVEND 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 117 EqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqlSDLFTLIYTSGTTGEPKGVML 196
Cdd:cd17640 87 S----------------------------------------------------------DDLATIIYTSGTTGNPKGVML 108
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 197 DYANLAHQLnaHDLALNVNED--DVSLSFLPLSHIFERAWIAYVFHRGATNCYlEDTKHVRDALTTLKPTVMCAVPRFYE 274
Cdd:cd17640 109 THANLLHQI--RSLSDIVPPQpgDRFLSILPIWHSYERSAEYFIFACGCSQAY-TSIRTLKDDLKRVKPHYIVSVPRLWE 185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 275 KIYTAVWDKVEKAPLHRRALFnwairvgeqhyqieqpsqwlrlqyaladklvltkLRALLGGRIKMMPCGGAKLEASIGS 354
Cdd:cd17640 186 SLYSGIQKQVSKSSPIKQFLF----------------------------------LFFLSGGIFKFGISGGGALPPHVDT 231
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 355 FFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEE 423
Cdd:cd17640 232 FFEAIGIEVLNGYGLTETSPVVSARRLKCNVRGSVGRPLPGTEIKIvdpegnvvlppGEKGIVWVRGPQVMKGYYKNPEA 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 424 TAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFD 503
Cdd:cd17640 312 TSKVLDSDGWFNTGDLGWLTCGGELVLTGRAKDTIVLSNGENVEPQPIEEALMRSPFIEQIMVVGQDQKRLGALIVPNFE 391
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 504 SLEEYAKQLNIKY-QDRIELIKHSDIIQMFERRIHELQKELP---SFEQVKRFTLLPQAFsTAMEEITPTLKLRRKVI 577
Cdd:cd17640 392 ELEKWAKESGVKLaNDRSQLLASKKVLKLYKNEIKDEISNRPgfkSFEQIAPFALLEEPF-IENGEMTQTMKIKRNVV 468
|
|
| ACSBG_like |
cd05933 |
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ... |
28-589 |
1.14e-100 |
|
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341256 [Multi-domain] Cd Length: 596 Bit Score: 317.76 E-value: 1.14e-100
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 28 REQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDAD 107
Cdd:cd05933 1 KRGDKWHTLTYKEYYEACRQAAKAFLKLGLERFHGVGILGFNSPEWFIAAVGAIFAGGIAVGIYTTNSPEACQYVAETSE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 108 IKILFVGDQEQFDQVYQIANNCPQLMKIVAMKANMHLRdLPNACYWEDF--LNVVPNEAEFEKRLNSKQLSDLFTLIYTS 185
Cdd:cd05933 81 ANILVVENQKQLQKILQIQDKLPHLKAIIQYKEPLKEK-EPNLYSWDEFmeLGRSIPDEQLDAIISSQKPNQCCTLIYTS 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 186 GTTGEPKGVMLDYANL---AHQLNAH-DLALNVNEDDVSLSFLPLSHIFER---AWIAyVFHRGATncYLEDtkhvRDAL 258
Cdd:cd05933 160 GTTGMPKGVMLSHDNItwtAKAASQHmDLRPATVGQESVVSYLPLSHIAAQildIWLP-IKVGGQV--YFAQ----PDAL 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 259 -----TTLK---PTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVGEQHYQIEQPSQW-LRLQYALADKLVLTK 329
Cdd:cd05933 233 kgtlvKTLRevrPTAFMGVPRVWEKIQEKMKAVGAKSGTLKRKIASWAKGVGLETNLKLMGGESpSPLFYRLAKKLVFKK 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 330 LRALLG-GRIKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTA--TVScwQDKGFNPNSIGTLMPNAEVKIGEEN-- 404
Cdd:cd05933 313 VRKALGlDRCQKFFTGAAPISRETLEFFLSLNIPIMELYGMSETSGphTIS--NPQAYRLLSCGKALPGCKTKIHNPDad 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 ---EILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKD-KF 480
Cdd:cd05933 391 gigEICFWGRHVFMGYLNMEDKTEEAIDEDGWLHSGDLGKLDEDGFLYITGRIKELIITAGGENVPPVPIEDAVKKElPI 470
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 481 IEQIAVIADAKKYVSALI-VPC---------FDSLE----EYAKQLNIKYQDRIELIKHSD--IIQMFERRIHELQKELP 544
Cdd:cd05933 471 ISNAMLIGDKRKFLSMLLtLKCevnpetgepLDELTeeaiEFCRKLGSQATRVSEIAGGKDpkVYEAIEEGIKRVNKKAI 550
|
570 580 590 600
....*....|....*....|....*....|....*....|....*.
gi 544685990 545 SFEQ-VKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMY 589
Cdd:cd05933 551 SNAQkIQKWVILEKDFSVPGGELGPTMKLKRPVVAKKYKDEIDKLY 596
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
12-461 |
1.01e-97 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 304.24 E-value: 1.01e-97
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 12 FRLQAKKWLNRTALRFREqaqWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:pfam00501 1 LERQAARTPDKTALEVGE---GRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLN 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 92 ATNTAKQVEYIVNDADIKILFVGDQEQFDQVYQIANNCPQLMKIVAMKANMHLRDlpnacywEDFLNVVPNEAEFEKRLN 171
Cdd:pfam00501 78 PRLPAEELAYILEDSGAKVLITDDALKLEELLEALGKLEVVKLVLVLDRDPVLKE-------EPLPEEAKPADVPPPPPP 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 172 SKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAH----DLALNVNEDDVSLSFLPLSHIFERAWIAY-VFHRGATNC 246
Cdd:pfam00501 151 PPDPDDLAYIIYTSGTTGKPKGVMLTHRNLVANVLSIkrvrPRGFGLGPDDRVLSTLPLFHDFGLSLGLLgPLLAGATVV 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 247 YLE-----DTKHVRDALTTLKPTVMCAVPRFYEKIytavwdkVEKAPLHRRALfnwairvgeqhyqieqpsqwlrlqyal 321
Cdd:pfam00501 231 LPPgfpalDPAALLELIERYKVTVLYGVPTLLNML-------LEAGAPKRALL--------------------------- 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 322 adklvltklrallgGRIKMMPCGGAKLEASIGSFFHSIGIN-IKLGYGMTETTATVSC---WQDKGFNPNSIGTLMPNAE 397
Cdd:pfam00501 277 --------------SSLRLVLSGGAPLPPELARRFRELFGGaLVNGYGLTETTGVVTTplpLDEDLRSLGSVGRPLPGTE 342
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 398 VKI-----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTS 461
Cdd:pfam00501 343 VKIvddetgepvppGEPGELCVRGPGVMKGYLNDPELTAEAFDEDGWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
32-581 |
9.04e-96 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 302.08 E-value: 9.04e-96
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 32 QWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKIL 111
Cdd:cd05932 3 QVVEFTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKAL 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 112 FVGdqeQFDQVYQIANNCPQLMKIVAMkanmHLRDLPNACY-WEDFLNVVPNEAEFEKRLNSKqlsdLFTLIYTSGTTGE 190
Cdd:cd05932 83 FVG---KLDDWKAMAPGVPEGLISISL----PPPSAANCQYqWDDLIAQHPPLEERPTRFPEQ----LATLIYTSGTTGQ 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 191 PKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLEDT--KHVRDaLTTLKPTVMCA 268
Cdd:cd05932 152 PKGVMLTFGSFAWAAQAGIEHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLVAFAESldTFVED-VQRARPTLFFS 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 269 VPRFYEKIYTAVWDKVEKAPLHRRalfnwairvgeqhyqieqpsqwlrLQYALADKLVLTKLRALLG-GRIKMMPCGGAK 347
Cdd:cd05932 231 VPRLWTKFQQGVQDKIPQQKLNLL------------------------LKIPVVNSLVKRKVLKGLGlDQCRLAGCGSAP 286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 348 LEASIGSFFHSIGINIKLGYGMTETTAtVSCWQDKGFNP-NSIGTLMPNAEVKIGEENEILVRGGMVMRGYYKKPEETAK 426
Cdd:cd05932 287 VPPALLEWYRSLGLNILEAYGMTENFA-YSHLNYPGRDKiGTVGNAGPGVEVRISEDGEILVRSPALMMGYYKDPEATAE 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 427 AFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPcfdSLE 506
Cdd:cd05932 366 AFTADGFLRTGDKGELDADGNLTITGRVKDIFKTSKGKYVAPAPIENKLAEHDRVEMVCVIGSGLPAPLALVVL---SEE 442
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 507 EYAKQLNikyQDRIELIKHsdiiqmFERRIHELQKELPSFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRY 581
Cdd:cd05932 443 ARLRADA---FARAELEAS------LRAHLARVNSTLDSHEQLAGIVVVKDPWSIDNGILTPTLKIKRNVLEKAY 508
|
|
| PLN02736 |
PLN02736 |
long-chain acyl-CoA synthetase |
26-591 |
6.92e-93 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 178337 [Multi-domain] Cd Length: 651 Bit Score: 298.94 E-value: 6.92e-93
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 26 RFREQAQWQEMSWQTFQQEIDR---FSYALIAQHIDIQDKIGIFANNMPRWTIADFgAMQARA-VAVPIYATNTAKQVEY 101
Cdd:PLN02736 66 RIRVDGTVGEYKWMTYGEAGTArtaIGSGLVQHGIPKGACVGLYFINRPEWLIVDH-ACSAYSyVSVPLYDTLGPDAVKF 144
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 102 IVNDADIKILFVGDQeQFDQVYQIANNCPQLMKIVAMK-ANMHLRDLPNACYWEdflnVVP-NEAEFEKRLNSKQL---- 175
Cdd:PLN02736 145 IVNHAEVAAIFCVPQ-TLNTLLSCLSEIPSVRLIVVVGgADEPLPSLPSGTGVE----IVTySKLLAQGRSSPQPFrppk 219
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 176 -SDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERA-WIAYVFHRGATNCYLEDTKH 253
Cdd:PLN02736 220 pEDVATICYTSGTTGTPKGVVLTHGNLIANVAGSSLSTKFYPSDVHISYLPLAHIYERVnQIVMLHYGVAVGFYQGDNLK 299
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 254 VRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVGEQhyQIEQ---PSqwlrlqyALADKLVLTKL 330
Cdd:PLN02736 300 LMDDLAALRPTIFCSVPRLYNRIYDGITNAVKESGGLKERLFNAAYNAKKQ--ALENgknPS-------PMWDRLVFNKI 370
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 331 RALLGGRIKMMPCGGAKLEASIGSFFH-SIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVK---IGEEN-- 404
Cdd:PLN02736 371 KAKLGGRVRFMSSGASPLSPDVMEFLRiCFGGRVLEGYGMTETSCVISGMDEGDNLSGHVGSPNPACEVKlvdVPEMNyt 450
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 ---------EILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKI 475
Cdd:PLN02736 451 sedqpyprgEICVRGPIIFKGYYKDEVQTREVIDEDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGEYIAPEKIENVY 530
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 476 GKDKFIEQIAVIADA-KKYVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFERRIHELQKE--LPSFEQVKRF 552
Cdd:PLN02736 531 AKCKFVAQCFVYGDSlNSSLVAVVVVDPEVLKAWAASEGIKYEDLKQLCNDPRVRAAVLADMDAVGREaqLRGFEFAKAV 610
|
570 580 590
....*....|....*....|....*....|....*....
gi 544685990 553 TLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMYNE 591
Cdd:PLN02736 611 TLVPEPFTVENGLLTPTFKVKRPQAKAYFAKAISDMYAE 649
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
162-577 |
2.94e-89 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 285.26 E-value: 2.94e-89
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 162 NEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNA--HDLALNVNEDDVSLSFLPLSHIFERAWIAYVF 239
Cdd:cd17639 74 NETECSAIFTDGKPDDLACIMYTSGSTGNPKGVMLTHGNLVAGIAGlgDRVPELLGPDDRYLAYLPLAHIFELAAENVCL 153
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 240 HRGATNCY-----LED--TKHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAirvgeqhYQIEqpS 312
Cdd:cd17639 154 YRGGTIGYgsprtLTDksKRGCKGDLTEFKPTLMVGVPAIWDTIRKGVLAKLNPMGGLKRTLFWTA-------YQSK--L 224
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 313 QWLRLQY--ALADKLVLTKLRALLGGRIKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTA--TVSCWQDkgFNPNS 388
Cdd:cd17639 225 KALKEGPgtPLLDELVFKKVRAALGGRLRYMLSGGAPLSADTQEFLNIVLCPVIQGYGLTETCAggTVQDPGD--LETGR 302
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 389 IGTLMPNAEVKIGE-------------ENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLK 455
Cdd:cd17639 303 VGPPLPCCEIKLVDweeggystdkpppRGEILIRGPNVFKGYYKNPEKTKEAFDGDGWFHTGDIGEFHPDGTLKIIDRKK 382
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 456 ELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIAD-AKKYVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFER 534
Cdd:cd17639 383 DLVKLQNGEYIALEKLESIYRSNPLVNNICVYADpDKSYPVAIVVPNEKHLTKLAEKHGVINSEWEELCEDKKLQKAVLK 462
|
410 420 430 440
....*....|....*....|....*....|....*....|....*
gi 544685990 535 RIHELQKE--LPSFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVI 577
Cdd:cd17639 463 SLAETARAagLEKFEIPQGVVLLDEEWTPENGLVTAAQKLKRKEI 507
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
35-574 |
1.35e-87 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 279.33 E-value: 1.35e-87
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 35 EMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVG 114
Cdd:cd05914 7 PLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKAIFVS 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 115 DQEqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqlsDLFTLIYTSGTTGEPKGV 194
Cdd:cd05914 87 DED-----------------------------------------------------------DVALINYTSGTTGNSKGV 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 195 MLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAW-IAYVFHRGATNCYLEDTKHVR-DALTTLKPTVMCAVPRF 272
Cdd:cd05914 108 MLTYRNIVSNVDGVKEVVLLGKGDKILSILPLHHIYPLTFtLLLPLLNGAHVVFLDKIPSAKiIALAFAQVTPTLGVPVP 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 273 Y--EKIYtavwdKVEKAPLHRRALFNWAIRVGEQHYQIEqpsqwlrlqyaladKLVLTKLRALLGGRIKMMPCGGAKLEA 350
Cdd:cd05914 188 LviEKIF-----KMDIIPKLTLKKFKFKLAKKINNRKIR--------------KLAFKKVHEAFGGNIKEFVIGGAKINP 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 351 SIGSFFHSIGINIKLGYGMTETTATVScwqdkgFNP------NSIGTLMPNAEVKI------GEENEILVRGGMVMRGYY 418
Cdd:cd05914 249 DVEEFLRTIGFPYTIGYGMTETAPIIS------YSPpnrirlGSAGKVIDGVEVRIdspdpaTGEGEIIVRGPNVMKGYY 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 419 KKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVsALI 498
Cdd:cd05914 323 KNPEATAEAFDKDGWFHTGDLGKIDAEGYLYIRGRKKEMIVLSSGKNIYPEEIEAKINNMPFVLESLVVVQEKKLV-ALA 401
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 499 VPCFDSLEEYAKQLnikyQDRIELIKhsdiiqmfERRIHELQKELPSFEQVKRFTLLPQAFstameEITPTLKLRR 574
Cdd:cd05914 402 YIDPDFLDVKALKQ----RNIIDAIK--------WEVRDKVNQKVPNYKKISKVKIVKEEF-----EKTPKGKIKR 460
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
12-589 |
9.37e-83 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 272.46 E-value: 9.37e-83
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 12 FRLQAKKWLNRTALRFRE--QAQWQEMSWQTFQQ---EIDRFSYALIAQHIDIQDKIGIFANNMPRWTIAdfgaMQARA- 85
Cdd:PLN02430 48 FSKSVEKYPDNKMLGWRRivDGKVGPYMWKTYKEvyeEVLQIGSALRASGAEPGSRVGIYGSNCPQWIVA----MEACAa 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 86 ---VAVPIYATNTAKQVEYIVNDADIKILFVGDQeqfdQVYQIAN-NCPQLMKIVAMKANMHLRDLP---------NACY 152
Cdd:PLN02430 124 hslICVPLYDTLGPGAVDYIVDHAEIDFVFVQDK----KIKELLEpDCKSAKRLKAIVSFTSVTEEEsdkasqigvKTYS 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 153 WEDFLNVvpnEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNED-----DVSLSFLPLS 227
Cdd:PLN02430 200 WIDFLHM---GKENPSETNPPKPLDICTIMYTSGTSGDPKGVVLTHEAVATFVRGVDLFMEQFEDkmthdDVYLSFLPLA 276
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 228 HIFERAWIAYVFHRGATNCYLE-DTKHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRvgeqhY 306
Cdd:PLN02430 277 HILDRMIEEYFFRKGASVGYYHgDLNALRDDLMELKPTLLAGVPRVFERIHEGIQKALQELNPRRRLIFNALYK-----Y 351
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 307 QIeqpsQWLRLQYA------LADKLVLTKLRALLGGRIKMMPCGGAKLEASIGSFFH--SIGINIKlGYGMTETTATVS- 377
Cdd:PLN02430 352 KL----AWMNRGYShkkaspMADFLAFRKVKAKLGGRLRLLISGGAPLSTEIEEFLRvtSCAFVVQ-GYGLTETLGPTTl 426
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 378 CWQDKGFNPNSIGTLMPNAEVKIGE-------------ENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDS 444
Cdd:PLN02430 427 GFPDEMCMLGTVGAPAVYNELRLEEvpemgydplgeppRGEICVRGKCLFSGYYKNPELTEEVM-KDGWFHTGDIGEILP 505
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 445 CGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADA-KKYVSALIVPCFDSLEEYAKQLNIKyqDRIELI 523
Cdd:PLN02430 506 NGVLKIIDRKKNLIKLSQGEYVALEYLENVYGQNPIVEDIWVYGDSfKSMLVAVVVPNEENTNKWAKDNGFT--GSFEEL 583
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 544685990 524 KHSDiiQMFERRIHELQ-----KELPSFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMY 589
Cdd:PLN02430 584 CSLP--ELKEHILSELKstaekNKLRGFEYIKGVILETKPFDVERDLVTATLKKRRNNLLKYYQVEIDEMY 652
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
12-587 |
4.14e-78 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 254.35 E-value: 4.14e-78
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 12 FRLQAKKWLNRTALRFreqaQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:COG0318 5 LRRAAARHPDRPALVF----GGRRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLN 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 92 ATNTAKQVEYIVNDADIKILFVgdqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrln 171
Cdd:COG0318 81 PRLTAEELAYILEDSGARALVT---------------------------------------------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 172 skqlsdlFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFerAWIAYVF---HRGATNCYL 248
Cdd:COG0318 103 -------ALILYTSGTTGRPKGVMLTHRNLLANAAAIAAALGLTPGDVVLVALPLFHVF--GLTVGLLaplLAGATLVLL 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 249 E--DTKHVRDALTTLKPTVMCAVPRFYEKIytavwdkvekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADKLV 326
Cdd:COG0318 174 PrfDPERVLELIERERVTVLFGVPTMLARL----------------------------------------LRHPEFARYD 213
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 327 LTKLRALLggrikmmpCGGAKL-EASIGSFFHSIGINIKLGYGMTETTATVSC-WQDKGFN-PNSIGTLMPNAEVKI--- 400
Cdd:COG0318 214 LSSLRLVV--------SGGAPLpPELLERFEERFGVRIVEGYGLTETSPVVTVnPEDPGERrPGSVGRPLPGVEVRIvde 285
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNGKYIAPQYIEG 473
Cdd:COG0318 286 dgrelppGEVGEIVVRGPNVMKGYWNDPEATAEAF-RDGWLRTGDLGRLDEDGYLYIVGRKKDMII-SGGENVYPAEVEE 363
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 474 KIGKDKFIEQIAVIA--DAKKY--VSALIVPCFDSLEeyakqlnikyqDRIELIKHsdiiqmferriheLQKELPSFEQV 549
Cdd:COG0318 364 VLAAHPGVAEAAVVGvpDEKWGerVVAFVVLRPGAEL-----------DAEELRAF-------------LRERLARYKVP 419
|
570 580 590 600
....*....|....*....|....*....|....*....|
gi 544685990 550 KRFTLLpqafstamEEI--TPTLKLRRKVIMQRYREQIEE 587
Cdd:COG0318 420 RRVEFV--------DELprTASGKIDRRALRERYAAGALE 451
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
38-591 |
2.50e-76 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 255.54 E-value: 2.50e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 38 WQTFQQEID---RFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVg 114
Cdd:PLN02861 77 WLTYKEVYDaaiRIGSAIRSRGVNPGDRCGIYGSNCPEWIIAMEACNSQGITYVPLYDTLGANAVEFIINHAEVSIAFV- 155
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 115 DQEQFDQVYQIANNCPQLMKI------VAMKANMHLRDLPNACY-WEDFLNVVPNEAEfekrLNSKQLSDLFTLIYTSGT 187
Cdd:PLN02861 156 QESKISSILSCLPKCSSNLKTivsfgdVSSEQKEEAEELGVSCFsWEEFSLMGSLDCE----LPPKQKTDICTIMYTSGT 231
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 188 TGEPKGVMLDYANLAHQLNAHDLALNVN-----EDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLE-DTKHVRDALTTL 261
Cdd:PLN02861 232 TGEPKGVILTNRAIIAEVLSTDHLLKVTdrvatEEDSYFSYLPLAHVYDQVIETYCISKGASIGFWQgDIRYLMEDVQAL 311
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 262 KPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIrvgeqHYQIEQPSQWLRLQYA--LADKLVLTKLRALLGGRIK 339
Cdd:PLN02861 312 KPTIFCGVPRVYDRIYTGIMQKISSGGMLRKKLFDFAY-----NYKLGNLRKGLKQEEAspRLDRLVFDKIKEGLGGRVR 386
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 340 MMPCGGAKLEASIGSFFHSIGI-NIKLGYGMTETTAtvSCWQDKGFNPNSIGTL---MPNAEVKIGE------------- 402
Cdd:PLN02861 387 LLLSGAAPLPRHVEEFLRVTSCsVLSQGYGLTESCG--GCFTSIANVFSMVGTVgvpMTTIEARLESvpemgydalsdvp 464
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 403 ENEILVRGGMVMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIE 482
Cdd:PLN02861 465 RGEICLRGNTLFSGYHKRQDLTEEVLI-DGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSQGEYVAVENLENTYSRCPLIA 543
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 483 QIAVIADA-KKYVSALIVPCFDSLEEYAKQLNiKYQDRIELIKHSDIIQMFERRIHELQKE--LPSFEQVKRFTLLPQAF 559
Cdd:PLN02861 544 SIWVYGNSfESFLVAVVVPDRQALEDWAANNN-KTGDFKSLCKNLKARKYILDELNSTGKKlqLRGFEMLKAIHLEPNPF 622
|
570 580 590
....*....|....*....|....*....|..
gi 544685990 560 STAMEEITPTLKLRRKVIMQRYREQIEEMYNE 591
Cdd:PLN02861 623 DIERDLITPTFKLKRPQLLKYYKDCIDQLYSE 654
|
|
| PLN02614 |
PLN02614 |
long-chain acyl-CoA synthetase |
12-589 |
6.52e-73 |
|
long-chain acyl-CoA synthetase
Pssm-ID: 166255 [Multi-domain] Cd Length: 666 Bit Score: 246.47 E-value: 6.52e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 12 FRLQAKKWLNRTALRFRE--QAQWQEMSWQTFQQEID---RFSYALIAQHIDIQDKIGIFANNMPRWTIAdFGAMQARAV 86
Cdd:PLN02614 51 FRMSVEKYPNNPMLGRREivDGKPGKYVWQTYQEVYDiviKLGNSLRSVGVKDEAKCGIYGANSPEWIIS-MEACNAHGL 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 87 -AVPIYATNTAKQVEYIVNDADIKILFVgDQEQFDQVYQIANNCPQLMKIVAMKANMHLRDLPNA-------CYWEDFLN 158
Cdd:PLN02614 130 yCVPLYDTLGAGAVEFIISHSEVSIVFV-EEKKISELFKTCPNSTEYMKTVVSFGGVSREQKEEAetfglviYAWDEFLK 208
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 159 VVPNEaefEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLD-------YANLAHQLNAHDLALNvnEDDVSLSFLPLSHIFE 231
Cdd:PLN02614 209 LGEGK---QYDLPIKKKSDICTIMYTSGTTGDPKGVMISnesivtlIAGVIRLLKSANAALT--VKDVYLSYLPLAHIFD 283
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 232 RAWI-AYVFHRGATNCYLEDTKHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWA-------IRVGE 303
Cdd:PLN02614 284 RVIEeCFIQHGAAIGFWRGDVKLLIEDLGELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFVFDSAfsykfgnMKKGQ 363
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 304 QHYQIEqpsqwlrlqyALADKLVLTKLRALLGGRIKMMPCGGAKLEASIGSFFHSIGI-NIKLGYGMTETTA-TVSCWQD 381
Cdd:PLN02614 364 SHVEAS----------PLCDKLVFNKVKQGLGGNVRIILSGAAPLASHVESFLRVVACcHVLQGYGLTESCAgTFVSLPD 433
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 382 KGFNPNSIGTLMPNAEVKIGE-------------ENEILVRGGMVMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNL 448
Cdd:PLN02614 434 ELDMLGTVGPPVPNVDIRLESvpemeydalastpRGEICIRGKTLFSGYYKREDLTKEVLI-DGWLHTGDVGEWQPNGSM 512
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 449 FITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADA-KKYVSALIVPCFDSLEEYAKQ--LNIKYQDRIELIKH 525
Cdd:PLN02614 513 KIIDRKKNIFKLSQGEYVAVENIENIYGEVQAVDSVWVYGNSfESFLVAIANPNQQILERWAAEngVSGDYNALCQNEKA 592
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 544685990 526 SDIIqMFERRIHELQKELPSFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMY 589
Cdd:PLN02614 593 KEFI-LGELVKMAKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMY 655
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
12-511 |
1.80e-66 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 225.45 E-value: 1.80e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 12 FRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIy 91
Cdd:PRK06187 12 LRHGARKHPDKEAVYFDGR----RTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAVLHPI- 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 92 atNT---AKQVEYIVNDADIKILFVgDQEQFDQVYQIANNCPQLMKIVAMKANMHLRDLPNACYWEDFLNVVPNEAEF-E 167
Cdd:PRK06187 87 --NIrlkPEEIAYILNDAEDRVVLV-DSEFVPLLAAILPQLPTVRTVIVEGDGPAAPLAPEVGEYEELLAAASDTFDFpD 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 168 KRLNskqlsDLFTLIYTSGTTGEPKGVMLDYANL-AHQLNAHDlALNVNEDDVSLSFLPLSHIFerAW-IAYV-FHRGAT 244
Cdd:PRK06187 164 IDEN-----DAAAMLYTSGTTGHPKGVVLSHRNLfLHSLAVCA-WLKLSRDDVYLVIVPMFHVH--AWgLPYLaLMAGAK 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 245 NCYLE--DTKHVRDALTTLKPTVMCAVPrfyekiytAVWDKVEKAPLHRRALFNwairvgeqhyqieqpsqwlrlqyala 322
Cdd:PRK06187 236 QVIPRrfDPENLLDLIETERVTFFFAVP--------TIWQMLLKAPRAYFVDFS-------------------------- 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 323 dklvltklrallggRIKMMPCGGAKL-EASIGSFFHSIGINIKLGYGMTETTATVSC----WQDKGFNP--NSIGTLMPN 395
Cdd:PRK06187 282 --------------SLRLVIYGGAALpPALLREFKEKFGIDLVQGYGMTETSPVVSVlppeDQLPGQWTkrRSAGRPLPG 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 396 AEVKI------------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNG 463
Cdd:PRK06187 348 VEARIvdddgdelppdgGEVGEIIVRGPWLMQGYWNRPEATAETI-DGGWLHTGDVGYIDEDGYLYITDRIKDVII-SGG 425
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 464 KYIAPQYIEGKIGKDKFIEQIAVIA--DAK--KYVSALIVPC------FDSLEEYAKQ 511
Cdd:PRK06187 426 ENIYPRELEDALYGHPAVAEVAVIGvpDEKwgERPVAVVVLKpgatldAKELRAFLRG 483
|
|
| PLN02387 |
PLN02387 |
long-chain-fatty-acid-CoA ligase family protein |
35-589 |
9.58e-64 |
|
long-chain-fatty-acid-CoA ligase family protein
Pssm-ID: 215217 [Multi-domain] Cd Length: 696 Bit Score: 222.30 E-value: 9.58e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 35 EMSWQTFQQEIDR---FSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKIL 111
Cdd:PLN02387 103 EYEWITYGQVFERvcnFASGLVALGHNKEERVAIFADTRAEWLIALQGCFRQNITVVTIYASLGEEALCHSLNETEVTTV 182
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 112 fVGDQEQFDQVYQIANNCPQLMKIVAMKANMHLRD--LPNACYW--EDFLNV--VPNEAEFEKRLNSKqlSDLFTLIYTS 185
Cdd:PLN02387 183 -ICDSKQLKKLIDISSQLETVKRVIYMDDEGVDSDssLSGSSNWtvSSFSEVekLGKENPVDPDLPSP--NDIAVIMYTS 259
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 186 GTTGEPKGVMLDYANLAHQLNAHDLAL-NVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCY-----LEDT-----KHV 254
Cdd:PLN02387 260 GSTGLPKGVMMTHGNIVATVAGVMTVVpKLGKNDVYLAYLPLAHILELAAESVMAAVGAAIGYgspltLTDTsnkikKGT 339
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 255 RDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAI--RVGeqhyQIEqpSQWL---RLQYALADKLVLTK 329
Cdd:PLN02387 340 KGDASALKPTLMTAVPAILDRVRDGVRKKVDAKGGLAKKLFDIAYkrRLA----AIE--GSWFgawGLEKLLWDALVFKK 413
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 330 LRALLGGRIKMMPCGGAKLEASIGSFFH-SIGINIKLGYGMTETTA--TVSCWQDKgfNPNSIGTLMPNAEVKI--GEE- 403
Cdd:PLN02387 414 IRAVLGGRIRFMLSGGAPLSGDTQRFINiCLGAPIGQGYGLTETCAgaTFSEWDDT--SVGRVGPPLPCCYVKLvsWEEg 491
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 404 -----------NEILVRGGMVMRGYYKKPEETAKAFTEDG----FLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAP 468
Cdd:PLN02387 492 gylisdkpmprGEIVIGGPSVTLGYFKNQEKTDEVYKVDErgmrWFYTGDIGQFHPDGCLEIIDRKKDIVKLQHGEYVSL 571
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 469 QYIEGKIGKDKFIEQIAVIADA-KKYVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFERRIHELQKE--LPS 545
Cdd:PLN02387 572 GKVEAALSVSPYVDNIMVHADPfHSYCVALVVPSQQALEKWAKKAGIDYSNFAELCEKEEAVKEVQQSLSKAAKAarLEK 651
|
570 580 590 600
....*....|....*....|....*....|....*....|....
gi 544685990 546 FEQVKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMY 589
Cdd:PLN02387 652 FEIPAKIKLLPEPWTPESGLVTAALKLKREQIRKKFKDDLKKLY 695
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
34-487 |
3.20e-62 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 213.23 E-value: 3.20e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 34 QEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFV 113
Cdd:cd05911 9 KELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVIFT 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 114 gDQEQFDQVYQIANNCPQLMKIVAMKAnmHLRDLPNACYWEDFLNVVPNEAEFEKRLNSKQlsDLFTLIYTSGTTGEPKG 193
Cdd:cd05911 89 -DPDGLEKVKEAAKELGPKDKIIVLDD--KPDGVLSIEDLLSPTLGEEDEDLPPPLKDGKD--DTAAILYSSGTTGLPKG 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 194 VMLDYANL---AHQLNAHdLALNVNEDDVSLSFLPLSHIFerawiayvfhrGATNCYLedtkhvrdALTTLKPTVMCavP 270
Cdd:cd05911 164 VCLSHRNLianLSQVQTF-LYGNDGSNDVILGFLPLYHIY-----------GLFTTLA--------SLLNGATVIIM--P 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 RFYekiyTAVW-DKVEKaplhrralfnwairvgeqhYQIEQ----PSQWLRL-QYALADKLVLTKLRALLggrikmmpCG 344
Cdd:cd05911 222 KFD----SELFlDLIEK-------------------YKITFlylvPPIAAALaKSPLLDKYDLSSLRVIL--------SG 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 345 GAKLEASIGSFFHSIGIN--IKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI-----------GEENEILVRGG 411
Cdd:cd05911 271 GAPLSKELQELLAKRFPNatIKQGYGMTETGGILTVNPDGDDKPGSVGRLLPNVEAKIvdddgkdslgpNEPGEICVRGP 350
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 412 MVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:cd05911 351 QVMKGYYNNPEATKETFDEDGWLHTGDIGYFDEDGYLYIVDRKKELIKY-KGFQVAPAELEAVLLEHPGVADAAVI 425
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
20-487 |
1.19e-60 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 209.76 E-value: 1.19e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 20 LNRTALRFREQAQWQEM----SWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNT 95
Cdd:PRK07656 11 LARAARRFGDKEAYVFGdqrlTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLNTRYT 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 96 AKQVEYIVNDADIKILFVgdQEQF-DQVYQIANNCPQLMKIVAMKANMHLRDLPNACYWEDFLNvVPNEAEFEKRLNSKQ 174
Cdd:PRK07656 91 ADEAAYILARGDAKALFV--LGLFlGVDYSATTRLPALEHVVICETEEDDPHTEKMKTFTDFLA-AGDPAERAPEVDPDD 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 175 LSDlftLIYTSGTTGEPKGVMLDYANLAhqLNAHDLA--LNVNEDDVSLSFLPLSHIF--ERAWIAyVFHRGATnCYLED 250
Cdd:PRK07656 168 VAD---ILFTSGTTGRPKGAMLTHRQLL--SNAADWAeyLGLTEGDRYLAANPFFHVFgyKAGVNA-PLMRGAT-ILPLP 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 251 TKHVRDALTTL---KPTVMCAVPRFYekiytavwdkvekaplhrralfnwairvgeqhyqieqpsQWLrLQYALADKLVL 327
Cdd:PRK07656 241 VFDPDEVFRLIeteRITVLPGPPTMY---------------------------------------NSL-LQHPDRSAEDL 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 328 TKLRALLGGRIKMMPcggakleASIGSFFHSIGINIKL-GYGMTETTATVS-CWQDKGFN--PNSIGTLMPNAEVKI--- 400
Cdd:PRK07656 281 SSLRLAVTGAASMPV-------ALLERFESELGVDIVLtGYGLSEASGVTTfNRLDDDRKtvAGTIGTAIAGVENKIvne 353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEG 473
Cdd:PRK07656 354 lgeevpvGEVGELLVRGPNVMKGYYDDPEATAAAIDADGWLHTGDLGRLDEEGYLYIVDRKKD-MFIVGGFNVYPAEVEE 432
|
490
....*....|....
gi 544685990 474 KIGKDKFIEQIAVI 487
Cdd:PRK07656 433 VLYEHPAVAEAAVI 446
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
15-487 |
9.07e-58 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 200.87 E-value: 9.07e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 15 QAKKWLNRTALRFREQaqwqemsWQTFQQ---EIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:cd05936 8 AARRFPDKTALIFMGR-------KLTYREldaLAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLN 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 92 ATNTAKQVEYIVNDADIKILFVGDqeqfdqvyqianncpqlmkivamkanmhlrdlpnacYWEDFLnvVPNEAEFEKRLN 171
Cdd:cd05936 81 PLYTPRELEHILNDSGAKALIVAV------------------------------------SFTDLL--AAGAPLGERVAL 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 172 SKQlsDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVN--EDDVSLSFLPLSHIFerAWIA---YVFHRGATNC 246
Cdd:cd05936 123 TPE--DVAVLQYTSGTTGVPKGAMLTHRNLVANALQIKAWLEDLleGDDVVLAALPLFHVF--GLTVallLPLALGATIV 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 247 YLE--DTKHVRDALTTLKPTVMCAVPRFYEKIytavwdkvekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADK 324
Cdd:cd05936 199 LIPrfRPIGVLKEIRKHRVTIFPGVPTMYIAL----------------------------------------LNAPEFKK 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 325 LVLTKLRALLggrikmmpCGGAKLEASIGSFFHSI-GINIKLGYGMTETTATVSC----WQDKgfnPNSIGTLMPNAEVK 399
Cdd:cd05936 239 RDFSSLRLCI--------SGGAPLPVEVAERFEELtGVPIVEGYGLTETSPVVAVnpldGPRK---PGSIGIPLPGTEVK 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 400 I----------GEENEILVRGGMVMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQ 469
Cdd:cd05936 308 IvdddgeelppGEVGELWVRGPQVMKGYWNRPEETAEAFV-DGWLRTGDIGYMDEDGYFFIVDRKKD-MIIVGGFNVYPR 385
|
490
....*....|....*...
gi 544685990 470 YIEGKIGKDKFIEQIAVI 487
Cdd:cd05936 386 EVEEVLYEHPAVAEAAVV 403
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
177-591 |
9.24e-58 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 205.98 E-value: 9.24e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLI-YTSGTTGEPKGVMLDYANLAHQLNAHDLALN-----VNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCY--- 247
Cdd:PTZ00216 264 DDLALImYTSGTTGDPKGVMHTHGSLTAGILALEDRLNdligpPEEDETYCSYLPLAHIMEFGVTNIFLARGALIGFgsp 343
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 248 ---LEDTKHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAirvgeqhYQIeqpsqwlRLQyALAD- 323
Cdd:PTZ00216 344 rtlTDTFARPHGDLTEFRPVFLIGVPRIFDTIKKAVEAKLPPVGSLKRRVFDHA-------YQS-------RLR-ALKEg 408
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 324 -------KLVLTKLRALLGGRIKMMPCGGAKLEASIGSFFHSI-GINIKlGYGMTETTAtVSCWQDKG-FNPNSIGTLMP 394
Cdd:PTZ00216 409 kdtpywnEKVFSAPRAVLGGRVRAMLSGGGPLSAATQEFVNVVfGMVIQ-GWGLTETVC-CGGIQRTGdLEPNAVGQLLK 486
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 395 NAEVKI--GEE----------NEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSN 462
Cdd:PTZ00216 487 GVEMKLldTEEykhtdtpeprGEILLRGPFLFKGYYKQEELTREVLDEDGWFHTGDVGSIAANGTLRIIGRVKALAKNCL 566
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 463 GKYIAPQYIEGKIGKDKFI--EQIAVIAD-AKKYVSALIVPCFDSLEEYAKQLNIK------YQDRIELIKHSDIIQMFE 533
Cdd:PTZ00216 567 GEYIALEALEALYGQNELVvpNGVCVLVHpARSYICALVLTDEAKAMAFAKEHGIEgeypaiLKDPEFQKKATESLQETA 646
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 534 RRIHElqkelPSFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMYNE 591
Cdd:PTZ00216 647 RAAGR-----KSFEIVRHVRVLSDEWTPENGVLTAAMKLKRRVIDERYADLIKELFAD 699
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
177-501 |
2.23e-56 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 193.27 E-value: 2.23e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLE--DTKHV 254
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHIGGLFGLLGALLAGGTVVLLPkfDPEAA 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 255 RDALTTLKPTVMCAVPRFYEKIytavwdkvekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADKLVLTKLRALL 334
Cdd:cd04433 81 LELIEREKVTILLGVPTLLARL----------------------------------------LKAPESAGYDLSSLRALV 120
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 335 ggrikmmpCGGAKLEASIGSFFHSI-GINIKLGYGMTETTATVSCWQ--DKGFNPNSIGTLMPNAEVKI----------G 401
Cdd:cd04433 121 --------SGGAPLPPELLERFEEApGIKLVNGYGLTETGGTVATGPpdDDARKPGSVGRPVPGVEVRIvdpdggelppG 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 402 EENEILVRGGMVMRGYYKKPEETAkAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNGKYIAPQYIEGKIGKDKFI 481
Cdd:cd04433 193 EIGELVVRGPSVMKGYWNNPEATA-AVDEDGWYRTGDLGRLDEDGYLYIVGRLKDMIK-SGGENVYPAEVEAVLLGHPGV 270
|
330 340
....*....|....*....|....
gi 544685990 482 EQIAVIA--DAKK--YVSALIVPC 501
Cdd:cd04433 271 AEAAVVGvpDPEWgeRVVAVVVLR 294
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
21-500 |
2.66e-53 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 187.82 E-value: 2.66e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:cd17631 10 DRTALVFGGR----SLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTPPEVA 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFvgdqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqlSDLFT 180
Cdd:cd17631 86 YILADSGAKVLF---------------------------------------------------------------DDLAL 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFE-RAWIAYVFHRGATNCYLE--DTKHVRDA 257
Cdd:cd17631 103 LMYTSGTTGRPKGAMLTHRNLLWNAVNALAALDLGPDDVLLVVAPLFHIGGlGVFTLPTLLRGGTVVILRkfDPETVLDL 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 258 LTTLKPTVMCAVPrfyekiytAVWDKVekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADKLVLTKLRALLggr 337
Cdd:cd17631 183 IERHRVTSFFLVP--------TMIQAL--------------------------------LQHPRFATTDLSSLRAVI--- 219
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 338 ikmmpCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVsCW------QDKgfnPNSIGTLMPNAEVKI----------G 401
Cdd:cd17631 220 -----YGGAPMPERLLRALQARGVKFVQGYGMTETSPGV-TFlspedhRRK---LGSAGRPVFFVEVRIvdpdgrevppG 290
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 402 EENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDKFI 481
Cdd:cd17631 291 EVGEIVVRGPHVMAGYWNRPEATAAAF-RDGWFHTGDLGRLDEDGYLYIVDRKKD-MIISGGENVYPAEVEDVLYEHPAV 368
|
490 500
....*....|....*....|...
gi 544685990 482 EQIAVIA--DAK--KYVSALIVP 500
Cdd:cd17631 369 AEVAVIGvpDEKwgEAVVAVVVP 391
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
16-500 |
1.66e-45 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 168.99 E-value: 1.66e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 16 AKKWLNRTALRFREQAqwqeMSWQTFQQEIDRFSyALIAQHIDIQ--DKIGIFANNMPRWTIADFGAMQARAVAVPIYAT 93
Cdd:PRK08314 20 ARRYPDKTAIVFYGRA----ISYRELLEEAERLA-GYLQQECGVRkgDRVLLYMQNSPQFVIAYYAILRANAVVVPVNPM 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 94 NTAKQVEYIVNDADIKILFVGdQEQFDQVYQIANNCPQLMKIVA-----------------MKANMHLRDLPN--ACYWE 154
Cdd:PRK08314 95 NREEELAHYVTDSGARVAIVG-SELAPKVAPAVGNLRLRHVIVAqysdylpaepeiavpawLRAEPPLQALAPggVVAWK 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 155 DFL--NVVPNEAEfekrlnsKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHI--F 230
Cdd:PRK08314 174 EALaaGLAPPPHT-------AGPDDLAVLPYTSGTTGVPKGCMHTHRTVMANAVGSVLWSNSTPESVVLAVLPLFHVtgM 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 231 ERAWIAYVFhRGATncyledtkhvrdalttlkpTVMcaVPRfyekiytavWDKvEKAplhRRALfnwairvgeQHYQIeq 310
Cdd:PRK08314 247 VHSMNAPIY-AGAT-------------------VVL--MPR---------WDR-EAA---ARLI---------ERYRV-- 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 311 pSQWLRLQYALADKLV--------LTKLRaLLGGrikmmpcGGAKLEASIGSFFHSI-GINIKLGYGMTETTATVScwqd 381
Cdd:PRK08314 281 -THWTNIPTMVVDFLAspglaerdLSSLR-YIGG-------GGAAMPEAVAERLKELtGLDYVEGYGLTETMAQTH---- 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 382 kgFNP------NSIG--------------TLmpnAEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTE-DG--FLRTGD 438
Cdd:PRK08314 348 --SNPpdrpklQCLGiptfgvdarvidpeTL---EELPPGEVGEIVVHGPQVFKGYWNRPEATAEAFIEiDGkrFFRTGD 422
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 439 VGEMDSCGNLFITDRLKELMKTSNGKyIAPQYIEGKIGKDKFIEQIAVIA--DAKK--YVSALIVP 500
Cdd:PRK08314 423 LGRMDEEGYFFITDRLKRMINASGFK-VWPAEVENLLYKHPAIQEACVIAtpDPRRgeTVKAVVVL 487
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
47-468 |
7.26e-44 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 163.56 E-value: 7.26e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 47 RFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgDQEQFDQVYQIA 126
Cdd:cd05904 44 RLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLSTPAEIAKQVKDSGAKLAFT-TAELAEKLASLA 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 127 NncpqlmKIVAMkanmhlRDLPNACYWEDFLNVVPNEAEFeKRLNSKQlSDLFTLIYTSGTTGEPKGVMLDYANL---AH 203
Cdd:cd05904 123 L------PVVLL------DSAEFDSLSFSDLLFEADEAEP-PVVVIKQ-DDVAALLYSSGTTGRSKGVMLTHRNLiamVA 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 204 QLNAhDLALNVNEDDVSLSFLPLSHIFERAWIAY-VFHRGATNCYLE--DTKHVRDALTTLKPTVMCAVPrfyeKIYTAV 280
Cdd:cd05904 189 QFVA-GEGSNSDSEDVFLCVLPMFHIYGLSSFALgLLRLGATVVVMPrfDLEELLAAIERYKVTHLPVVP----PIVLAL 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 281 WDKvekaplhrralfnwairvgeqhyqieqpsqwlrlqyALADKLVLTKLRALLggrikmmpCGGAKLEASIGSFFHSI- 359
Cdd:cd05904 264 VKS------------------------------------PIVDKYDLSSLRQIM--------SGAAPLGKELIEAFRAKf 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 360 -GINIKLGYGMTETTA-TVSCWQDKGF--NPNSIGTLMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEET 424
Cdd:cd05904 300 pNVDLGQGYGMTESTGvVAMCFAPEKDraKYGSVGRLVPNVEAKIvdpetgeslppNQTGELWIRGPSIMKGYLNNPEAT 379
|
410 420 430 440
....*....|....*....|....*....|....*....|....
gi 544685990 425 AKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAP 468
Cdd:cd05904 380 AATIDKEGWLHTGDLCYIDEDGYLFIVDRLKELIKY-KGFQVAP 422
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
35-499 |
2.17e-42 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 158.03 E-value: 2.17e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 35 EMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVG 114
Cdd:cd05935 1 SLTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVG 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 115 dqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnsKQLSDLFTLIYTSGTTGEPKGV 194
Cdd:cd05935 81 ----------------------------------------------------------SELDDLALIPYTSGTTGLPKGC 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 195 MLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHI--FERAWIAYVFhRGATNCYLE--DTKHVRDALTTLKPTVMCAVP 270
Cdd:cd05935 103 MHTHFSAAANALQSAVWTGLTPSDVILACLPLFHVtgFVGSLNTAVY-VGGTYVLMArwDRETALELIEKYKVTFWTNIP 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 rfyekiyTAVWDKVEKAPLHRRALfnwairvgeqhyqieqpsqwlrlqyaladklvlTKLRALLGGRIKMMPCGGAKLEA 350
Cdd:cd05935 182 -------TMLVDLLATPEFKTRDL---------------------------------SSLKVLTGGGAPMPPAVAEKLLK 221
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 351 SIGSFFHSiginiklGYGMTETTATV----------SCWQDKGFNPNS-IGTLMPNAEVKIGEENEILVRGGMVMRGYYK 419
Cdd:cd05935 222 LTGLRFVE-------GYGLTETMSQThtnpplrpklQCLGIP*FGVDArVIDIETGRELPPNEVGEIVVRGPQIFKGYWN 294
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 420 KPEETAKAFTEDG---FLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVIADAKKY--- 493
Cdd:cd05935 295 RPEETEESFIEIKgrrFFRTGDLGYMDEEGYFFFVDRVKRMINVS-GFKVWPAEVEAKLYKHPAI*EVCVISVPDERvge 373
|
....*..
gi 544685990 494 -VSALIV 499
Cdd:cd05935 374 eVKAFIV 380
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
34-488 |
2.49e-38 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 146.28 E-value: 2.49e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 34 QEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFV 113
Cdd:cd05934 2 RRWTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVVV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 114 gdqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqlsDLFTLIYTSGTTGEPKG 193
Cdd:cd05934 82 ---------------------------------------------------------------DPASILYTSGTTGPPKG 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 194 VMLDYANLAH--QLNAHDLALNvnEDDVSLSFLPLSHIFERAWIAYV-FHRGATnCYLEDTKHVRdalttlkptvmcavp 270
Cdd:cd05934 99 VVITHANLTFagYYSARRFGLG--EDDVYLTVLPLFHINAQAVSVLAaLSVGAT-LVLLPRFSAS--------------- 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 RFyekiytavWDKVEKaplHRRALFNWAIRVGEqhYQIEQPSQWLRLQYaladklvltKLRALlggrikmmpCGGAKLEA 350
Cdd:cd05934 161 RF--------WSDVRR---YGATVTNYLGAMLS--YLLAQPPSPDDRAH---------RLRAA---------YGAPNPPE 209
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 351 SIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI----------GEENEILVRG----GMvMRG 416
Cdd:cd05934 210 LHEEFEERFGVRLLEGYGMTETIVGVIGPRDEPRRPGSIGRPAPGYEVRIvdddgqelpaGEPGELVIRGlrgwGF-FKG 288
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 544685990 417 YYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:cd05934 289 YYNMPEATAEAM-RNGWFHTGDLGYRDADGFFYFVDRKKDMIRRR-GENISSAEVERAILRHPAVREAAVVA 358
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
21-472 |
9.17e-38 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 147.18 E-value: 9.17e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRFR-EQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQV 99
Cdd:COG0365 24 DKVALIWEgEDGEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAMLACARIGAVHSPVFPGFGAEAL 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 100 EYIVNDADIKILFVGD--------QEQFDQVYQIANNCPQLMKIVAMKANMHLRDLPNACYWEDFLNVVPNEAEFEkRLN 171
Cdd:COG0365 104 ADRIEDAEAKVLITADgglrggkvIDLKEKVDEALEELPSLEHVIVVGRTGADVPMEGDLDWDELLAAASAEFEPE-PTD 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 172 SkqlSDLFTLIYTSGTTGEPKGVMLDYAN-LAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAY-VFHRGATNCYLE 249
Cdd:COG0365 183 A---DDPLFILYTSGTTGKPKGVVHTHGGyLVHAATTAKYVLDLKPGDVFWCTADIGWATGHSYIVYgPLLNGATVVLYE 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 DTKHVRDALTTL------KPTVMCAVPRFYekiytavwdkvekaplhrRALFNWAIRVGEQHyqieqpsqwlrlqyalaD 323
Cdd:COG0365 260 GRPDFPDPGRLWeliekyGVTVFFTAPTAI------------------RALMKAGDEPLKKY-----------------D 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 324 klvLTKLRALLggrikmmpCGGAKLEASIGSFFHS-IGINIKLGYGMTETTAT-VSCWQDKGFNPNSIGTLMPNAEVKI- 400
Cdd:COG0365 305 ---LSSLRLLG--------SAGEPLNPEVWEWWYEaVGVPIVDGWGQTETGGIfISNLPGLPVKPGSMGKPVPGYDVAVv 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 ---------GEENEILVRG---GMvMRGYYKKPEETAKAF--TEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYI 466
Cdd:COG0365 374 dedgnpvppGEEGELVIKGpwpGM-FRGYWNDPERYRETYfgRFPGWYRTGDGARRDEDGYFWILGRSDDVINVS-GHRI 451
|
....*.
gi 544685990 467 APQYIE 472
Cdd:COG0365 452 GTAEIE 457
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
37-488 |
1.15e-37 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 146.24 E-value: 1.15e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 37 SWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgDQ 116
Cdd:cd12119 27 TYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHTINPRLFPEQIAYIINHAEDRVVFV-DR 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 117 EQFDQVYQIANNCPQLMKIVAM--KANMHLRDLPNACYWEDFLNVVPNEAEFeKRLNSKQLSdlfTLIYTSGTTGEPKGV 194
Cdd:cd12119 106 DFLPLLEAIAPRLPTVEHVVVMtdDAAMPEPAGVGVLAYEELLAAESPEYDW-PDFDENTAA---AICYTSGTTGNPKGV 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 195 MLDYANLAHQ---LNAHDlALNVNEDDVSLSFLPLSHIfeRAW-IAYV-FHRGA----TNCYLeDTKHVRDALTTLKPTV 265
Cdd:cd12119 182 VYSHRSLVLHamaALLTD-GLGLSESDVVLPVVPMFHV--NAWgLPYAaAMVGAklvlPGPYL-DPASLAELIEREGVTF 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 266 MCAVPrfyekiytAVWDKVekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADKLVLTKLRALLggrikmmpCGG 345
Cdd:cd12119 258 AAGVP--------TVWQGL--------------------------------LDHLEANGRDLSSLRRVV--------IGG 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 346 AKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPN-----------SIGTLMPNAEVKIGEEN---------- 404
Cdd:cd12119 290 SAVPRSLIEAFEERGVRVIHAWGMTETSPLGTVARPPSEHSNlsedeqlalraKQGRPVPGVELRIVDDDgrelpwdgka 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 --EILVRGGMVMRGYYKKPEETAkAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNGKYIAPQYIEGKIGKDKFIE 482
Cdd:cd12119 370 vgELQVRGPWVTKSYYKNDEESE-ALTEDGWLRTGDVATIDEDGYLTITDRSKDVIK-SGGEWISSVELENAIMAHPAVA 447
|
....*.
gi 544685990 483 QIAVIA 488
Cdd:cd12119 448 EAAVIG 453
|
|
| PTZ00342 |
PTZ00342 |
acyl-CoA synthetase; Provisional |
180-466 |
2.66e-37 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240370 [Multi-domain] Cd Length: 746 Bit Score: 147.56 E-value: 2.66e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 180 TLIYTSGTTGEPKGVMLDYANLAHQ---LNAHDLALNVNEDDvSLSFLPLSHIFERAWIAYVFHRGAT-NCYLEDTKHVR 255
Cdd:PTZ00342 308 SIVYTSGTSGKPKGVMLSNKNLYNTvvpLCKHSIFKKYNPKT-HLSYLPISHIYERVIAYLSFMLGGTiNIWSKDINYFS 386
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 256 DALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVGEQHYQIEQpSQWLrlqyalaDKL--VLTKLRAL 333
Cdd:PTZ00342 387 KDIYNSKGNILAGVPKVFNRIYTNIMTEINNLPPLKRFLVKKILSLRKSNNNGGF-SKFL-------EGIthISSKIKDK 458
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 334 LGGRIKMMPCGGAKLEASIGSFFhSIGINIKL--GYGMTETTATVSCWQDKGFNPNSIG-TLMPNAEVKIGE-------- 402
Cdd:PTZ00342 459 VNPNLEVILNGGGKLSPKIAEEL-SVLLNVNYyqGYGLTETTGPIFVQHADDNNTESIGgPISPNTKYKVRTwetykatd 537
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990 403 ---ENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYI 466
Cdd:PTZ00342 538 tlpKGELLIKSDSIFSGYFLEKEQTKNAFTEDGYFKTGDIVQINKNGSLTFLDRSKGLVKLSQGEYI 604
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
21-516 |
1.11e-36 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 142.04 E-value: 1.11e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQ-DKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQV 99
Cdd:cd05941 1 DRIAIVDDGD----SITYADLVARAARLANRLLALGKDLRgDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAEL 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 100 EYIVNDADIKILFvgdqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqlsDLF 179
Cdd:cd05941 77 EYVITDSEPSLVL----------------------------------------------------------------DPA 92
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 180 TLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHI-----------FERAWIayVFHRGATNCYL 248
Cdd:cd05941 93 LILYTSGTTGRPKGVVLTHANLAANVRALVDAWRWTEDDVLLHVLPLHHVhglvnallcplFAGASV--EFLPKFDPKEV 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 249 EDTKHVRDAlttlkpTVMCAVPRFYEKIytavwdkvekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADKLVLT 328
Cdd:cd05941 171 AISRLMPSI------TVFMGVPTIYTRL----------------------------------------LQYYEAHFTDPQ 204
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 KLRALLGGRIKMMPCGGAKLEASIGSFFHSIGINIKLG-YGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKIGEEN--- 404
Cdd:cd05941 205 FARAAAAERLRLMVSGSAALPVPTLEEWEAITGHTLLErYGMTEIGMALSNPLDGERRPGTVGMPLPGVQARIVDEEtge 284
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 --------EILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIG 476
Cdd:cd05941 285 plprgevgEIQVRGPSVFKEYWNKPEATKEEFTDDGWFKTGDLGVVDEDGYYWILGRSSVDIIKSGGYKVSALEIERVLL 364
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 544685990 477 KDKFIEQIAVI----ADAKKYVSALIVP-------CFDSLEEYAKQLNIKY 516
Cdd:cd05941 365 AHPGVSECAVIgvpdPDWGERVVAVVVLragaaalSLEELKEWAKQRLAPY 415
|
|
| ligase_PEP_1 |
TIGR03098 |
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ... |
23-472 |
6.58e-35 |
|
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.
Pssm-ID: 211788 [Multi-domain] Cd Length: 517 Bit Score: 138.38 E-value: 6.58e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 23 TALRFREQAqwqeMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYI 102
Cdd:TIGR03098 17 TALVHHDRT----LTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGVFVPINPLLKAEQVAHI 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 103 VNDADIKILfVGDQEQFDQVYQIANNCPQLMKIVAMKANMHLRDLP---NACYWEDFLNVVPneaefEKRLNSKQLSDLF 179
Cdd:TIGR03098 93 LADCNVRLL-VTSSERLDLLHPALPGCHDLRTLIIVGDPAHASEGHpgeEPASWPKLLALGD-----ADPPHPVIDSDMA 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 180 TLIYTSGTTGEPKGVMLDYANLAhqLNAHDLA--LNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATnCYLEDTKHVRDA 257
Cdd:TIGR03098 167 AILYTSGSTGRPKGVVLSHRNLV--AGAQSVAtyLENRPDDRLLAVLPLSFDYGFNQLTTAFYVGAT-VVLHDYLLPRDV 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 258 LTTLKP---TVMCAVPrfyekiytavwdkvekaPLhrralfnWAirvgeqhyqieqpsQWLRLQYALADKLVLTKLrALL 334
Cdd:TIGR03098 244 LKALEKhgiTGLAAVP-----------------PL-------WA--------------QLAQLDWPESAAPSLRYL-TNS 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 335 GGRikmMPcggAKLEASIGSFFHSigINIKLGYGMTEttATVSCWQDKGF---NPNSIGTLMPNAEVKI----------G 401
Cdd:TIGR03098 285 GGA---MP---RATLSRLRSFLPN--ARLFLMYGLTE--AFRSTYLPPEEvdrRPDSIGKAIPNAEVLVlredgsecapG 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 402 EENEILVRGGMVMRGYYKKPEETAKAFT-----EDGFLRT------GDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQY 470
Cdd:TIGR03098 355 EEGELVHRGALVAMGYWNDPEKTAERFRplppfPGELHLPelavwsGDTVRRDEEGFLYFVGRRDEMIKTS-GYRVSPTE 433
|
..
gi 544685990 471 IE 472
Cdd:TIGR03098 434 VE 435
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
37-579 |
3.47e-34 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 135.90 E-value: 3.47e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 37 SWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQ 116
Cdd:cd05926 16 TYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSKLVLTPKG 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 117 EQFDqvyqianncpqlmkivAMKANMHLRDLPNACYWEDFLNVVPNEAE------FEKRLNSKQL----SDLFTLIYTSG 186
Cdd:cd05926 96 ELGP----------------ASRAASKLGLAILELALDVGVLIRAPSAEslsnllADKKNAKSEGvplpDDLALILHTSG 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 187 TTGEPKGVMLDYANLA---------HQLNAHDLALNVneddvslsfLPLSHIferawiayvfhrgatncyledtkH--VR 255
Cdd:cd05926 160 TTGRPKGVPLTHRNLAasatnitntYKLTPDDRTLVV---------MPLFHV-----------------------HglVA 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 256 DALTTL--KPTVMCAvPRFYEkiyTAVWDKVEKAplhrRAlfNWAIRVGEQHyQIeqpsqwlRLQYALADKL-VLTKLRA 332
Cdd:cd05926 208 SLLSTLaaGGSVVLP-PRFSA---STFWPDVRDY----NA--TWYTAVPTIH-QI-------LLNRPEPNPEsPPPKLRF 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 333 LLGGRIKMMPCGGAKLEAsigsFFhsiGINIKLGYGMTETTATVSC-----WQDKgfnPNSIGtlMPN-AEVKI------ 400
Cdd:cd05926 270 IRSCSASLPPAVLEALEA----TF---GAPVLEAYGMTEAAHQMTSnplppGPRK---PGSVG--KPVgVEVRIldedge 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 ----GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIG 476
Cdd:cd05926 338 ilppGVVGEICLRGPNVTRGYLNNPEANAEAAFKDGWFRTGDLGYLDADGYLFLTGRIKELINRG-GEKISPLEVDGVLL 416
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 477 KDKFIEQIAVIA--DAkKY---VSALIVPCFDSleeyakqlnikYQDRIELIKHsdiiqmferriheLQKELPSFEQVKR 551
Cdd:cd05926 417 SHPAVLEAVAFGvpDE-KYgeeVAAAVVLREGA-----------SVTEEELRAF-------------CRKHLAAFKVPKK 471
|
570 580 590
....*....|....*....|....*....|.
gi 544685990 552 FTL---LPQafstameeiTPTLKLRRKVIMQ 579
Cdd:cd05926 472 VYFvdeLPK---------TATGKIQRRKVAE 493
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
2-499 |
5.97e-34 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 135.40 E-value: 5.97e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 2 ASLDFHfvnrfrlqAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAM 81
Cdd:PRK06145 6 ASIAFH--------ARRTPDRAALVYRDQ----EISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAAS 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 82 QARAVAVPIYATNTAKQVEYIVNDADIKILFVgdQEQFDQVyqIANNCPQLMKIVAMKANMHLRDLPNAcywedflNVVP 161
Cdd:PRK06145 74 YLGAVFLPINYRLAADEVAYILGDAGAKLLLV--DEEFDAI--VALETPKIVIDAAAQADSRRLAQGGL-------EIPP 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 162 NEAEFEkrlnskqlSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHI--FERAWIAYVF 239
Cdd:PRK06145 143 QAAVAP--------TDLVRLMYTSGTTDRPKGVMHSYGNLHWKSIDHVIALGLTASERLLVVGPLYHVgaFDLPGIAVLW 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 240 HrGATNCYLEDTKhvrdalttlKPTVMCAVPRfyEKIyTAVWdkveKAPLHRRALFNWAIRvgeqhyqieqpsqwlrlqy 319
Cdd:PRK06145 215 V-GGTLRIHREFD---------PEAVLAAIER--HRL-TCAW----MAPVMLSRVLTVPDR------------------- 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 320 alaDKLVLTKLRALLGGrikmmpcgGAKLEASIGSFFHSIGINIKL--GYGMTETTATvSCWQDKGFNPNSIGTL---MP 394
Cdd:PRK06145 259 ---DRFDLDSLAWCIGG--------GEKTPESRIRDFTRVFTRARYidAYGLTETCSG-DTLMEAGREIEKIGSTgraLA 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 395 NAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGK 464
Cdd:PRK06145 327 HVEIRIadgagrwlppNMKGEICMRGPKVTKGYWKDPEKTAEAFY-GDWFRSGDVGYLDEEGFLYLTDRKKD-MIISGGE 404
|
490 500 510
....*....|....*....|....*....|....*....
gi 544685990 465 YIAPQYIEGKIGKDKFIEQIAVIA--DAK--KYVSALIV 499
Cdd:PRK06145 405 NIASSEVERVIYELPEVAEAAVIGvhDDRwgERITAVVV 443
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
75-501 |
6.74e-34 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 135.15 E-value: 6.74e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 75 IADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQ--EQFDQvyqianncPQLMKIVAMKANMHLRDLPNACY 152
Cdd:cd05909 46 LANFALALSGKVPVMLNYTAGLRELRACIKLAGIKTVLTSKQfiEKLKL--------HHLFDVEYDARIVYLEDLRAKIS 117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 153 WED----FL--NVVPNEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPL 226
Cdd:cd05909 118 KADkckaFLagKFPPKWLLRIFGVAPVQPDDPAVILFTSGSEGLPKGVVLSHKNLLANVEQITAIFDPNPEDVVFGALPF 197
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 227 SHIFERA---W------IAYVFHRGATncyleDTKHVRDALTTLKPTVMCAVPRFYeKIYTavwdkvekaplhRRAlfnw 297
Cdd:cd05909 198 FHSFGLTgclWlpllsgIKVVFHPNPL-----DYKKIPELIYDKKATILLGTPTFL-RGYA------------RAA---- 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 298 airvgeqhyqieQPSQWLRLQYALadklvltklrallggrikmmpCGGAKLEASIGSFFHS-IGINIKLGYGMTETTATV 376
Cdd:cd05909 256 ------------HPEDFSSLRLVV---------------------AGAEKLKDTLRQEFQEkFGIRILEGYGTTECSPVI 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 377 SCWQDK-GFNPNSIGTLMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDS 444
Cdd:cd05909 303 SVNTPQsPNKEGTVGRPLPGMEVKIvsvetheevpiGEGGLLLVRGPNVMLGYLNEPELTSFAF-GDGWYDTGDIGKIDG 381
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 445 CGNLFITDRLKELMKtsngkyiapqyIEGKIGKDKFIEQIA-VIADAKKYVSALIVPC 501
Cdd:cd05909 382 EGFLTITGRLSRFAK-----------IAGEMVSLEAIEDILsEILPEDNEVAVVSVPD 428
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
35-487 |
1.11e-33 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 134.96 E-value: 1.11e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 35 EMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVg 114
Cdd:cd17642 44 NYSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNERELDHSLNISKPTIVFC- 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 115 DQEQFDQVYQIANNCPQLMKIVAMKANMHLRDLPNAcywEDFL--NVVPNEAEFEKRLNSKQLSDLFTLI-YTSGTTGEP 191
Cdd:cd17642 123 SKKGLQKVLNVQKKLKIIKTIIILDSKEDYKGYQCL---YTFItqNLPPGFNEYDFKPPSFDRDEQVALImNSSGSTGLP 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 192 KGVMLDYANLAHQLN-AHD--LALNVNEDDVSLSFLPLSHIFerawiayvfhrgatncyledtkhvrdALTTLKPTVMCA 268
Cdd:cd17642 200 KGVQLTHKNIVARFShARDpiFGNQIIPDTAILTVIPFHHGF--------------------------GMFTTLGYLICG 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 269 -----VPRFYEKIYTavwdkvekaplhrRALfnwairvgeQHYQIEQ----PSQWLRL-QYALADKLVLTKLRALLggri 338
Cdd:cd17642 254 frvvlMYKFEEELFL-------------RSL---------QDYKVQSallvPTLFAFFaKSTLVDKYDLSNLHEIA---- 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 339 kmmpCGGAKLEASIGSFF-HSIGIN-IKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI-----------GEENE 405
Cdd:cd17642 308 ----SGGAPLSKEVGEAVaKRFKLPgIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFYAKVvdldtgktlgpNERGE 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 406 ILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGKIGKDKFIEQIA 485
Cdd:cd17642 384 LCVKGPMIMKGYVNNPEATKALIDKDGWLHSGDIAYYDEDGHFFIVDRLKSLIKY-KGYQVPPAELESILLQHPKIFDAG 462
|
..
gi 544685990 486 VI 487
Cdd:cd17642 463 VA 464
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
21-512 |
4.61e-33 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 133.36 E-value: 4.61e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRFREQAQwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:PRK12583 33 DREALVVRHQAL--RYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVNINPAYRASELE 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFVGD----------------QEQFDQVYQIAN-NCPQLMKIVAMKANmhlrDLPNACYWEDFL---NVV 160
Cdd:PRK12583 111 YALGQSGVRWVICADafktsdyhamlqellpGLAEGQPGALACeRLPELRGVVSLAPA----PPPGFLAWHELQargETV 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 161 PNEAEFEkRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFER--AWIAYV 238
Cdd:PRK12583 187 SREALAE-RQASLDRDDPINIQYTSGTTGFPKGATLSHHNILNNGYFVAESLGLTEHDRLCVPVPLYHCFGMvlANLGCM 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 239 FHrGATNCYLEDTkhvRDALTTLKP------TVMCAVPrfyeKIYTAvwdkvekaplhrralfnwairvgeqhyQIEQPs 312
Cdd:PRK12583 266 TV-GACLVYPNEA---FDPLATLQAveeercTALYGVP----TMFIA---------------------------ELDHP- 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 313 qwlrlQYALADklvLTKLR-ALLGGrikmMPCGGAKLEASIGSFFHSigiNIKLGYGMTETT--ATVSCWQDK-GFNPNS 388
Cdd:PRK12583 310 -----QRGNFD---LSSLRtGIMAG----APCPIEVMRRVMDEMHMA---EVQIAYGMTETSpvSLQTTAADDlERRVET 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 389 IGTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKElM 458
Cdd:PRK12583 375 VGRTQPHLEVKVvdpdgatvprGEIGELCTRGYSVMKGYWNNPEATAESIDEDGWMHTGDLATMDEQGYVRIVGRSKD-M 453
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 544685990 459 KTSNGKYIAPQYIEgkigkDKFIEQIAVIAdakkyVSALIVPCfdslEEYAKQL 512
Cdd:PRK12583 454 IIRGGENIYPREIE-----EFLFTHPAVAD-----VQVFGVPD----EKYGEEI 493
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
84-489 |
3.51e-32 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 129.48 E-value: 3.51e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 84 RAVAVPIYATNTAKQVEYIVNDADIKILfvgdqeqfdqvyqianncpqlmkIVAMKANMHLRDLPNACY----WEDFLNV 159
Cdd:cd05922 46 GLVFVPLNPTLKESVLRYLVADAGGRIV-----------------------LADAGAADRLRDALPASPdpgtVLDADGI 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 160 VPNEAEFEKRLNSKQlsDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVF 239
Cdd:cd05922 103 RAARASAPAHEVSHE--DLALLLYTSGSTGSPKLVRLSHQNLLANARSIAEYLGITADDRALTVLPLSYDYGLSVLNTHL 180
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 240 HRGAT----NCYLEDTKHVrDALTTLKPTVMCAVPRFYEKIYTAVWDKvEKAPlHRRALFNWAIRVgeqhyqieqPSQWL 315
Cdd:cd05922 181 LRGATlvltNDGVLDDAFW-EDLREHGATGLAGVPSTYAMLTRLGFDP-AKLP-SLRYLTQAGGRL---------PQETI 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 316 RlqyALADKLVltklrallGGRIKMMpcggakleasigsffhsiginiklgYGMTETTATVSCW--QDKGFNPNSIGTLM 393
Cdd:cd05922 249 A---RLRELLP--------GAQVYVM-------------------------YGQTEATRRMTYLppERILEKPGSIGLAI 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 394 PNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnG 463
Cdd:cd05922 293 PGGEFEIldddgtptppGEPGEIVHRGPNVMKGYWNDPPYRRKEGRGGGVLHTGDLARRDEDGFLFIVGRRDRMIKLF-G 371
|
410 420
....*....|....*....|....*.
gi 544685990 464 KYIAPQYIEGKIGKDKFIEQIAVIAD 489
Cdd:cd05922 372 NRISPTEIEAAARSIGLIIEAAAVGL 397
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
54-508 |
4.18e-31 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 127.96 E-value: 4.18e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 54 AQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgDQEQFDQVYQIANNCPQLM 133
Cdd:cd17632 87 EQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAPILAETEPRLLAV-SAEHLDLAVEAVLEGGTPP 165
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 134 KIVAM--------------KANMHLRDLPNACYWEDFLNVVPNEAEFEKRLNSKQLSD-LFTLIYTSGTTGEPKGVMLDY 198
Cdd:cd17632 166 RLVVFdhrpevdahraaleSARERLAAVGIPVTTLTLIAVRGRDLPPAPLFRPEPDDDpLALLIYTSGSTGTPKGAMYTE 245
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 199 ANLAHQLnahdLALNVNEDD-----VSLSFLPLSHIFERAWIAYVFHRGATNCYL--EDTKHVRDALTTLKPTVMCAVPR 271
Cdd:cd17632 246 RLVATFW----LKVSSIQDIrppasITLNFMPMSHIAGRISLYGTLARGGTAYFAaaSDMSTLFDDLALVRPTELFLVPR 321
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 272 fyekiytaVWDKVekaplhrralfnwairvgEQHYQIEQpsQWLRLQYALADKL---VLTKLRA-LLGGRIKMMPCGGAK 347
Cdd:cd17632 322 --------VCDML------------------FQRYQAEL--DRRSVAGADAETLaerVKAELRErVLGGRLLAAVCGSAP 373
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 348 LEASIGSFFHS-IGINIKLGYGMTETtatvscwqdkgfnpnsiGTLMPNAEVK--------------IG--------EEN 404
Cdd:cd17632 374 LSAEMKAFMESlLDLDLHDGYGSTEA-----------------GAVILDGVIVrppvldyklvdvpeLGyfrtdrphPRG 436
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 EILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVgeMDSCG--NLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIE 482
Cdd:cd17632 437 ELLVKTDTLFPGYYKRPEVTAEVFDEDGFYRTGDV--MAELGpdRLVYVDRRNNVLKLSQGEFVTVARLEAVFAASPLVR 514
|
490 500
....*....|....*....|....*..
gi 544685990 483 QIAVIAD-AKKYVSALIVPCFDSLEEY 508
Cdd:cd17632 515 QIFVYGNsERAYLLAVVVPTQDALAGE 541
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
21-575 |
9.47e-31 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 124.95 E-value: 9.47e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:cd05930 2 DAVAVVDGDQ----SLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERLA 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFVgdqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskQLSDLFT 180
Cdd:cd05930 78 YILEDSGAKLVLT------------------------------------------------------------DPDDLAY 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS---HIFE--RAWIAyvfhrGATnCYLEDTKHVR 255
Cdd:cd05930 98 VIYTSGSTGKPKGVMVEHRGLVNLLLWMQEAYPLTPGDRVLQFTSFSfdvSVWEifGALLA-----GAT-LVVLPEEVRK 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 256 D------ALTTLKPTVMCAVPRFYekiytavwdkvekaplhrRALFNWAirvgeqhyqieqpsqwlrlqyalaDKLVLTK 329
Cdd:cd05930 172 DpealadLLAEEGITVLHLTPSLL------------------RLLLQEL------------------------ELAALPS 209
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 330 LRALLggrikmmpCGGAKLEASIGSFFHSIGINIKL--GYGMTETTATVSCW----QDKGFNPNSIGTLMPNAE------ 397
Cdd:cd05930 210 LRLVL--------VGGEALPPDLVRRWRELLPGARLvnLYGPTEATVDATYYrvppDDEEDGRVPIGRPIPNTRvyvlde 281
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 398 ----VKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFL------RTGDVGEMDSCGNLFITDRLKELMKTsNGKYIA 467
Cdd:cd05930 282 nlrpVPPGVPGELYIGGAGLARGYLNRPELTAERFVPNPFGpgermyRTGDLVRWLPDGNLEFLGRIDDQVKI-RGYRIE 360
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 468 PQYIEGKIGKDKFIEQIAVIA----DAKKYVSALIVPcfdsleeyakqlnikyqDRIELIKHSDIIQmferrihELQKEL 543
Cdd:cd05930 361 LGEIEAALLAHPGVREAAVVAredgDGEKRLVAYVVP-----------------DEGGELDEEELRA-------HLAERL 416
|
570 580 590
....*....|....*....|....*....|....
gi 544685990 544 PSFeqvkrftLLPQAFsTAMEEI--TPTLKLRRK 575
Cdd:cd05930 417 PDY-------MVPSAF-VVLDALplTPNGKVDRK 442
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
18-544 |
1.77e-30 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 125.92 E-value: 1.77e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 18 KWLNRTALRFREQAQW----QEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYAT 93
Cdd:PRK06710 28 KYVEQMASRYPEKKALhflgKDITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNPL 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 94 NTAKQVEYIVNDADIKILFVGD-------------QEQFDQVYQIANNCP---QLMKIVAMKANMHLRDLPNACYWEDFL 157
Cdd:PRK06710 108 YTERELEYQLHDSGAKVILCLDlvfprvtnvqsatKIEHVIVTRIADFLPfpkNLLYPFVQKKQSNLVVKVSESETIHLW 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 158 NVVPNEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQ--LNAHDLALNVNEDDVSLSFLPLSHIFerawi 235
Cdd:PRK06710 188 NSVEKEVNTGVEVPCDPENDLALLQYTGGTTGFPKGVMLTHKNLVSNtlMGVQWLYNCKEGEEVVLGVLPFFHVY----- 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 236 ayvfhrGATNCYledtkhvrdALTTLKPTVMCAVPRFYEKIytaVWDKVEKaplHRRALFNWAirvgeqhyqieqPSQWL 315
Cdd:PRK06710 263 ------GMTAVM---------NLSIMQGYKMVLIPKFDMKM---VFEAIKK---HKVTLFPGA------------PTIYI 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 316 RL-QYALADKLVLTKLRALLGGrikmmpcgGAKLEASIGSFFHSI-GINIKLGYGMTETTATVSC---WQDKgfNPNSIG 390
Cdd:PRK06710 310 ALlNSPLLKEYDISSIRACISG--------SAPLPVEVQEKFETVtGGKLVEGYGLTESSPVTHSnflWEKR--VPGSIG 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 391 TLMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEETAkAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMK 459
Cdd:PRK06710 380 VPWPDTEAMImsletgealppGEIGEIVVKGPQIMKGYWNKPEETA-AVLQDGWLHTGDVGYMDEDGFFYVKDRKKDMIV 458
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 460 TSnGKYIAPQYIEGKIGKDKFIEQIAVIADAKKY----VSALIVPCFDSL--EEYAKQLNIKYQDRIELIKhsdiiqmfe 533
Cdd:PRK06710 459 AS-GFNVYPREVEEVLYEHEKVQEVVTIGVPDPYrgetVKAFVVLKEGTEcsEEELNQFARKYLAAYKVPK--------- 528
|
570
....*....|.
gi 544685990 534 rrIHELQKELP 544
Cdd:PRK06710 529 --VYEFRDELP 537
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
46-453 |
4.56e-30 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 122.37 E-value: 4.56e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 46 DRFSYALIAQH-IDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQEQFDQVYQ 124
Cdd:TIGR01733 10 NRLARHLRAAGgVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDSALASRLAGL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 125 ianncpqlmkivamkanmhlrDLPNACYWEDFLNVVPNEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQ 204
Cdd:TIGR01733 90 ---------------------VLPVILLDPLELAALDDAPAPPPPDAPSGPDDLAYVIYTSGSTGRPKGVVVTHRSLVNL 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 205 LNAHDLALNVNEDDVSLSFLPLSH------IFeRAWIAyvfhrGATNCYLEDT------KHVRDALTTLKPTVMCAVPrf 272
Cdd:TIGR01733 149 LAWLARRYGLDPDDRVLQFASLSFdasveeIF-GALLA-----GATLVVPPEDeerddaALLAALIAEHPVTVLNLTP-- 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 273 yekiytAVWDKVEKAPLHRRALFNWAIRVGEqhyqieqpsqWLRLQyaladklVLTKLRAllggrikmmPCGGAKLeasi 352
Cdd:TIGR01733 221 ------SLLALLAAALPPALASLRLVILGGE----------ALTPA-------LVDRWRA---------RGPGARL---- 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 353 gsffhsigINiklGYGMTETTATVSCWQ-----DKGFNPNSIGTLMPNAE----------VKIGEENEILVRGGMVMRGY 417
Cdd:TIGR01733 265 --------IN---LYGPTETTVWSTATLvdpddAPRESPVPIGRPLANTRlyvldddlrpVPVGVVGELYIGGPGVARGY 333
|
410 420 430 440
....*....|....*....|....*....|....*....|....
gi 544685990 418 YKKPEETAKAFTEDGFL--------RTGDVGEMDSCGNLFITDR 453
Cdd:TIGR01733 334 LNRPELTAERFVPDPFAggdgarlyRTGDLVRYLPDGNLEFLGR 377
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
21-499 |
5.69e-30 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 124.34 E-value: 5.69e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAV---PIYatnTAK 97
Cdd:PRK05605 47 DRPALDFFGA----TTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAVVVehnPLY---TAH 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 98 QVEYIVNDADIKILFVGDQEQfDQVYQIANNCPqLMKIV------AMKANMHL------------RD-----LPNACYWE 154
Cdd:PRK05605 120 ELEHPFEDHGARVAIVWDKVA-PTVERLRRTTP-LETIVsvnmiaAMPLLQRLalrlpipalrkaRAaltgpAPGTVPWE 197
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 155 DFLNVVPNEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANL---AHQLNAHDLALnVNEDDVSLSFLPLSHIFE 231
Cdd:PRK05605 198 TLVDAAIGGDGSDVSHPRPTPDDVALILYTSGTTGKPKGAQLTHRNLfanAAQGKAWVPGL-GDGPERVLAALPMFHAYG 276
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 232 RAW-IAYVFHRGATNCYLE--DTKHVRDALTTLKPTVMCAVPRFYEKIYTAvwdkvekaplhrralfnwairvgeqhyqi 308
Cdd:PRK05605 277 LTLcLTLAVSIGGELVLLPapDIDLILDAMKKHPPTWLPGVPPLYEKIAEA----------------------------- 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 309 eqpsqwlrlqyALADKLVLTKLRALLGGRIKMMPCGGAKLEASIGSFfhsiginIKLGYGMTETTATVSCwqdkgfNPNS 388
Cdd:PRK05605 328 -----------AEERGVDLSGVRNAFSGAMALPVSTVELWEKLTGGL-------LVEGYGLTETSPIIVG------NPMS 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 389 -------IGTLMPNAEVKI------------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLF 449
Cdd:PRK05605 384 ddrrpgyVGVPFPDTEVRIvdpedpdetmpdGEEGELLVRGPQVFKGYWNRPEETAKSF-LDGWFRTGDVVVMEEDGFIR 462
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 544685990 450 ITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVI----ADAKKYVSALIV 499
Cdd:PRK05605 463 IVDRIKELIITG-GFNVYPAEVEEVLREHPGVEDAAVVglprEDGSEEVVAAVV 515
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
31-492 |
8.64e-30 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 124.00 E-value: 8.64e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 31 AQWQEMSwqtfqqeiDRFSyALIAQH-IDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIK 109
Cdd:PRK06178 62 AELDELS--------DRFA-ALLRQRgVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREHELSYELNDAGAE 132
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 110 ILFVGDQeQFDQVYQIANNCPQLMKIVAMKANM-----------HLRDLPNACY-WEDFLNVVPNEAEfEKRLNSKQLSD 177
Cdd:PRK06178 133 VLLALDQ-LAPVVEQVRAETSLRHVIVTSLADVlpaeptlplpdSLRAPRLAAAgAIDLLPALRACTA-PVPLPPPALDA 210
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 178 LFTLIYTSGTTGEPKGVMLDYANLAHQLNA-HDLALNVNEDDVSLSFLPlshIFeraWIA-------YVFHRGATNCYLe 249
Cdd:PRK06178 211 LAALNYTGGTTGMPKGCEHTQRDMVYTAAAaYAVAVVGGEDSVFLSFLP---EF---WIAgenfgllFPLFSGATLVLL- 283
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 dtkhVR-DALTtlkptVMCAVPRFYEKIYTAVWDK-VEKAPLHRRALFNwairvgeqhyqieqpsqwLR-LQYALADKLV 326
Cdd:PRK06178 284 ----ARwDAVA-----FMAAVERYRVTRTVMLVDNaVELMDHPRFAEYD------------------LSsLRQVRVVSFV 336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 327 LtKLRALLGGRIKMMpCGGAKLEASigsffhsiginiklgYGMTET-TA-TVSC-WQDKGFN----PNSIGTLMPNAEVK 399
Cdd:PRK06178 337 K-KLNPDYRQRWRAL-TGSVLAEAA---------------WGMTEThTCdTFTAgFQDDDFDllsqPVFVGLPVPGTEFK 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 400 I-----------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAP 468
Cdd:PRK06178 400 IcdfetgellplGAEGEIVVRTPSLLKGYWNKPEATAEAL-RDGWLHTGDIGKIDEQGFLHYLGRRKEMLKV-NGMSVFP 477
|
490 500
....*....|....*....|....*.
gi 544685990 469 QYIEGKIGKDKFIEQIAVI--ADAKK 492
Cdd:PRK06178 478 SEVEALLGQHPAVLGSAVVgrPDPDK 503
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
34-487 |
8.99e-30 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 123.05 E-value: 8.99e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 34 QEMSWQTFQQEIDRFSYALIAQ-HIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILF 112
Cdd:PRK06839 26 EEMTYKQLHEYVSKVAAYLIYElNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLTENELIFQLKDSGTTVLF 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 113 VgdQEQFdqvyqiANNCPQLMKIVAMKANMHLRDlpnacywedflnvvPNEAEFEKRLN--SKQLSDLFTLIYTSGTTGE 190
Cdd:PRK06839 106 V--EKTF------QNMALSMQKVSYVQRVISITS--------------LKEIEDRKIDNfvEKNESASFIICYTSGTTGK 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 191 PKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLshiferawiayvFHRGATNCYledtkhvrdALTTLKPTVMCAVP 270
Cdd:PRK06839 164 PKGAVLTQENMFWNALNNTFAIDLTMHDRSIVLLPL------------FHIGGIGLF---------AFPTLFAGGVIIVP 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 RFYEKiytavwDKvekaplhrralfnwAIRVGEQH-----YQIEQPSQWLRLqyalADKLVLTKLRAllggrIKMMPCGG 345
Cdd:PRK06839 223 RKFEP------TK--------------ALSMIEKHkvtvvMGVPTIHQALIN----CSKFETTNLQS-----VRWFYNGG 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 346 AKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGF--NPNSIGTLMPNAEVKI----------GEENEILVRGGMV 413
Cdd:PRK06839 274 APCPEELMREFIDRGFLFGQGFGMTETSPTVFMLSEEDArrKVGSIGKPVLFCDYELidenknkvevGEVGELLIRGPNV 353
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 544685990 414 MRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:PRK06839 354 MKEYWNRPDATEETI-QDGWLCTGDLARVDEDGFVYIVGRKKE-MIISGGENIYPLEVEQVINKLSDVYEVAVV 425
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
44-472 |
9.83e-30 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 123.72 E-value: 9.83e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 44 EIDRFSYALIA---QHIDIQ--DKIGIFANNMPRWTIADFGAMQARAVAV---PIYatnTAKQVEYIVNDADIKILF--- 112
Cdd:PRK05677 54 ELYKLSGAFAAwlqQHTDLKpgDRIAVQLPNVLQYPVAVFGAMRAGLIVVntnPLY---TAREMEHQFNDSGAKALVcla 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 113 --------VGDQEQFDQVY--QIANNCPQLMKIVAMKANMHLR------DLPNACyweDFLNVVPNEAEFEKRLNSKQLS 176
Cdd:PRK05677 131 nmahlaekVLPKTGVKHVIvtEVADMLPPLKRLLINAVVKHVKkmvpayHLPQAV---KFNDALAKGAGQPVTEANPQAD 207
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANL-AHQLNAHDL-ALNVNED-DVSLSFLPLSHIFerawiAYVFHRGATncyLEDTKH 253
Cdd:PRK05677 208 DVAVLQYTGGTTGVAKGAMLTHRNLvANMLQCRALmGSNLNEGcEILIAPLPLYHIY-----AFTFHCMAM---MLIGNH 279
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 254 vrdalttlkpTVMCAVPRfyekiytavwdkveKAPLHRRALFNWAIR--VGEQhyqieqpsqwlRLQYALADKlvlTKLR 331
Cdd:PRK05677 280 ----------NILISNPR--------------DLPAMVKELGKWKFSgfVGLN-----------TLFVALCNN---EAFR 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 332 ALLGGRIKMMPCGGAKLEASIGSFFHSI-GINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI---------- 400
Cdd:PRK05677 322 KLDFSALKLTLSGGMALQLATAERWKEVtGCAICEGYGMTETSPVVSVNPSQAIQVGTIGIPVPSTLCKVidddgnelpl 401
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 544685990 401 GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIE 472
Cdd:PRK05677 402 GEVGELCVKGPQVMKGYWQRPEATDEILDSDGWLKTGDIALIQEDGYMRIVDRKKDMILVS-GFNVYPNELE 472
|
|
| FCS |
cd05921 |
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ... |
22-591 |
1.62e-29 |
|
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.
Pssm-ID: 341245 [Multi-domain] Cd Length: 561 Bit Score: 122.93 E-value: 1.62e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 22 RTALRFREQAQ-WQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI---YATNTAK 97
Cdd:cd05921 11 RTWLAEREGNGgWRRVTYAEALRQVRAIAQGLLDLGLSAERPLLILSGNSIEHALMALAAMYAGVPAAPVspaYSLMSQD 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 98 --QVEYIVNDADIKILFVGDQEQFDQVyqIANNCPQLMKIVamkanmHLRDLPNACYWEDFLNVV--PNEAEFEKRLNSK 173
Cdd:cd05921 91 laKLKHLFELLKPGLVFAQDAAPFARA--LAAIFPLGTPLV------VSRNAVAGRGAISFAELAatPPTAAVDAAFAAV 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 174 QLSDLFTLIYTSGTTGEPKGVMLDYANLA--HQLNAHDLALNVNEDDVSLSFLPLSHIF-ERAWIAYVFHRGATnCYLED 250
Cdd:cd05921 163 GPDTVAKFLFTSGSTGLPKAVINTQRMLCanQAMLEQTYPFFGEEPPVLVDWLPWNHTFgGNHNFNLVLYNGGT-LYIDD 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 251 TKHVRDAL-TTLK------PTVMCAVPRFYEKIYTAVwdkvEKAPLHRRALFNwaiRVGEQHYQIEQPSQ--WLRLQyAL 321
Cdd:cd05921 242 GKPMPGGFeETLRnlreisPTVYFNVPAGWEMLVAAL----EKDEALRRRFFK---RLKLMFYAGAGLSQdvWDRLQ-AL 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 322 ADKLVltklrallGGRIKMMPcggakleasigsffhsiginiklGYGMTET--TATVSCW-QDKgfnPNSIGTLMPNAEV 398
Cdd:cd05921 314 AVATV--------GERIPMMA-----------------------GLGATETapTATFTHWpTER---SGLIGLPAPGTEL 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 399 KI---GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEM----DSCGNLFITDRLKELMKTSNGKYIA--PQ 469
Cdd:cd05921 360 KLvpsGGKYEVRVKGPNVTPGYWRQPELTAQAFDEEGFYCLGDAAKLadpdDPAKGLVFDGRVAEDFKLASGTWVSvgPL 439
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 470 YIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEEYAKqlnIKYQDRIELIKHSDIIQMFERRIHELQKEL-PSFEQ 548
Cdd:cd05921 440 RARAVAACAPLVHDAVVAGEDRAEVGALVFPDLLACRRLVG---LQEASDAEVLRHAKVRAAFRDRLAALNGEAtGSSSR 516
|
570 580 590 600
....*....|....*....|....*....|....*....|...
gi 544685990 549 VKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMYNE 591
Cdd:cd05921 517 IARALLLDEPPSIDKGEITDKGYINQRAVLERRAALVERLYAD 559
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
12-500 |
2.17e-29 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 122.61 E-value: 2.17e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 12 FRLQAKKWLNRTALRFREQ-AQWqemSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI 90
Cdd:PRK08315 22 LDRTAARYPDREALVYRDQgLRW---TYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFATAKIGAILVTI 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 91 yatNTA---KQVEYIVNDADIKILFVGDQEQ----FDQVYQIANNC-------------PQLMKIVAMKANMHlRDLPNa 150
Cdd:PRK08315 99 ---NPAyrlSELEYALNQSGCKALIAADGFKdsdyVAMLYELAPELatcepgqlqsarlPELRRVIFLGDEKH-PGMLN- 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 151 cyWEDFLN--VVPNEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHqlNAHDLALNVN---EDDVSLSfLP 225
Cdd:PRK08315 174 --FDELLAlgRAVDDAELAARQATLDPDDPINIQYTSGTTGFPKGATLTHRNILN--NGYFIGEAMKlteEDRLCIP-VP 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 226 LSHIFER--AWIAYVFHrGATNCYLEDtkhVRDALTTL------KPTVMCAVPrfyeKIYTAVWDkvekapLHRRALFNw 297
Cdd:PRK08315 249 LYHCFGMvlGNLACVTH-GATMVYPGE---GFDPLATLaaveeeRCTALYGVP----TMFIAELD------HPDFARFD- 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 298 airvgeqhyqieqpsqwlrlqyaladklvLTKLRallGGrikMM---PCGGAKLEASIGSFfHSIGINIklGYGMTETtA 374
Cdd:PRK08315 314 -----------------------------LSSLR---TG---IMagsPCPIEVMKRVIDKM-HMSEVTI--AYGMTET-S 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 375 TVSCwQDKGFNP-----NSIGTLMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGD 438
Cdd:PRK08315 355 PVST-QTRTDDPlekrvTTVGRALPHLEVKIvdpetgetvprGEQGELCTRGYSVMKGYWNDPEKTAEAIDADGWMHTGD 433
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 544685990 439 VGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEgkigkdKFIEQIAVIADA-------KKY---VSALIVP 500
Cdd:PRK08315 434 LAVMDEEGYVNIVGRIKD-MIIRGGENIYPREIE------EFLYTHPKIQDVqvvgvpdEKYgeeVCAWIIL 498
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
156-491 |
2.73e-29 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 120.14 E-value: 2.73e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 156 FLN--VVPNEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANlaHQLNAHDLALN--VNEDDVSLSFLPLSHI-- 229
Cdd:cd05912 55 LLNtrLTPNELAFQLKDSDVKLDDIATIMYTSGTTGKPKGVQQTFGN--HWWSAIGSALNlgLTEDDNWLCALPLFHIsg 132
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 230 ---FERAWIAyvfhrgATNCYLE---DTKHVRDALTTLKPTVMCAVPrfyekiytavwdkvekaplhrrALFNWAIRVGE 303
Cdd:cd05912 133 lsiLMRSVIY------GMTVYLVdkfDAEQVLHLINSGKVTIISVVP----------------------TMLQRLLEILG 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 304 QHYqieqPSQwLRLqyaladklvltklrALLGGRikmmPCGGAKLEASigsffHSIGINIKLGYGMTETtatvsCWQDKG 383
Cdd:cd05912 185 EGY----PNN-LRC--------------ILLGGG----PAPKPLLEQC-----KEKGIPVYQSYGMTET-----CSQIVT 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 384 FNP-------NSIGTLMPNAEVKI-------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLF 449
Cdd:cd05912 232 LSPedalnkiGSAGKPLFPVELKIeddgqppYEVGEILLKGPNVTKGYLNRPDATEESF-ENGWFKTGDIGYLDEEGFLY 310
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 544685990 450 ITDRLKELMkTSNGKYIAPQYIEGKIGKDKFIEQIAVIA--DAK 491
Cdd:cd05912 311 VLDRRSDLI-ISGGENIYPAEIEEVLLSHPAIKEAGVVGipDDK 353
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
176-493 |
3.43e-29 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 118.52 E-value: 3.43e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 176 SDLFTLIYTSGTTGEPKGVMLDYANL-AHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAY-VFHRGA--TNCYLEDT 251
Cdd:cd17635 1 EDPLAVIFTSGTTGEPKAVLLANKTFfAVPDILQKEGLNWVVGDVTYLPLPATHIGGLWWILTcLIHGGLcvTGGENTTY 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 252 KHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPlhrralfnwairvgeqhyqieqpsqwlrlqyaladklvltKLR 331
Cdd:cd17635 81 KSLFKILTTNAVTTTCLVPTLLSKLVSELKSANATVP----------------------------------------SLR 120
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 332 ALLGGrikmmpcGGAKLEASIGSFFHSIGINIKLGYGMTETTaTVSCWQ--DKGFNPNSIGTLMPNAEVKI--------- 400
Cdd:cd17635 121 LIGYG-------GSRAIAADVRFIEATGLTNTAQVYGLSETG-TALCLPtdDDSIEINAVGRPYPGVDVYLaatdgiagp 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -GEENEILVRGGMVMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEgkigkdK 479
Cdd:cd17635 193 sASFGTIWIKSPANMLGYWNNPERTAEVLI-DGWVNTGDLGERREDGFLFITGRSSESI-NCGGVKIAPDEVE------R 264
|
330
....*....|....
gi 544685990 480 FIEQIAVIADAKKY 493
Cdd:cd17635 265 IAEGVSGVQECACY 278
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
177-581 |
3.83e-29 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 118.20 E-value: 3.83e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIferAWIAYVfhrgatncyledtkhVRD 256
Cdd:cd17630 1 RLATVILTSGSTGTPKAVVHTAANLLASAAGLHSRLGFGGGDSWLLSLPLYHV---GGLAIL---------------VRS 62
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 257 ALTTLKPTVmcavprfyekiytavwdkvekaPLHRRALFNWAIRVGEQHYQIeQPSQwlrLQYALADKLV---LTKLRAL 333
Cdd:cd17630 63 LLAGAELVL----------------------LERNQALAEDLAPPGVTHVSL-VPTQ---LQRLLDSGQGpaaLKSLRAV 116
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 334 LggrikmmpCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKIGEENEILVRGGMV 413
Cdd:cd17630 117 L--------LGGAPIPPELLERAADRGIPLYTTYGMTETASQVATKRPDGFGRGGVGVLLPGRELRIVEDGEIWVGGASL 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 414 MRGYYKKPEEtaKAFTEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEgkigkdkfieqiAVIADAKKY 493
Cdd:cd17630 189 AMGYLRGQLV--PEFNEDGWFTTKDLGELHADGRLTVLGRADN-MIISGGENIQPEEIE------------AALAAHPAV 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 494 VSALIVPCFDslEEYAKQLNIKYQDRIELIkHSDIIQMferriheLQKELPSFEQVKRFTLLPQAfstameEITPTLKLR 573
Cdd:cd17630 254 RDAFVVGVPD--EELGQRPVAVIVGRGPAD-PAELRAW-------LKDKLARFKLPKRIYPVPEL------PRTGGGKVD 317
|
....*...
gi 544685990 574 RKVIMQRY 581
Cdd:cd17630 318 RRALRAWL 325
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
37-488 |
4.44e-29 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 120.87 E-value: 4.44e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 37 SWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQ 116
Cdd:cd12118 31 TWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNALNTRLDAEEIAFILRHSEAKVLFVDRE 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 117 EQFDQVYQIANncpqlmkivamkanmhlrdlpnacywEDFLNVVPNEaEFekrlnskqlsDLFTLIYTSGTTGEPKGVMl 196
Cdd:cd12118 111 FEYEDLLAEGD--------------------------PDFEWIPPAD-EW----------DPIALNYTSGTTGRPKGVV- 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 197 dYANLAHQLNAHDLAL--NVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLE--DTKHVRDALTTLKPTVMCAVPrf 272
Cdd:cd12118 153 -YHHRGAYLNALANILewEMKQHPVYLWTLPMFHCNGWCFPWTVAAVGGTNVCLRkvDAKAIYDLIEKHKVTHFCGAP-- 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 273 yekiytAVWDKVEKAPLHRRALFNWAIRVgeqhyqieqpsqwlrlqyaladklvLTklrallggrikmmpcGGAKLEASI 352
Cdd:cd12118 230 ------TVLNMLANAPPSDARPLPHRVHV-------------------------MT---------------AGAPPPAAV 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 353 GSFFHSIGINIKLGYGMTETT--ATVSCWQDK---------------------------GFNPNSIGTLmPNAEVKIGEe 403
Cdd:cd12118 264 LAKMEELGFDVTHVYGLTETYgpATVCAWKPEwdelpteerarlkarqgvryvgleevdVLDPETMKPV-PRDGKTIGE- 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 404 neILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEGKIGKDKFIEQ 483
Cdd:cd12118 342 --IVFRGNIVMKGYLKNPEATAEAF-RGGWFHSGDLAVIHPDGYIEIKDRSKDII-ISGGENISSVEVEGVLYKHPAVLE 417
|
....*
gi 544685990 484 IAVIA 488
Cdd:cd12118 418 AAVVA 422
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
12-500 |
1.60e-28 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 119.65 E-value: 1.60e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 12 FRLQAKKWLNRTALRFREQAqWqemSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:PRK08316 17 LRRSARRYPDKTALVFGDRS-W---TYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVN 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 92 ATNTAKQVEYIVNDADIKILFVGDQeqfdqVYQIANNCPQLMKIVAMKANMHLRDLPNACYWEDFLNVVPNE--AEFEKR 169
Cdd:PRK08316 93 FMLTGEELAYILDHSGARAFLVDPA-----LAPTAEAALALLPVDTLILSLVLGGREAPGGWLDFADWAEAGsvAEPDVE 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 170 LNSkqlSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSH-----IFERAWIaYVfhrGAT 244
Cdd:PRK08316 168 LAD---DDLAQILYTSGTESLPKGAMLTHRALIAEYVSCIVAGDMSADDIPLHALPLYHcaqldVFLGPYL-YV---GAT 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 245 NCYLE--DTKHVRDALTTLKPTVMCAVPrfyekiytAVWdkvekAPLHRRALFnwairvgeqhyqieqpsqwlrlqyala 322
Cdd:PRK08316 241 NVILDapDPELILRTIEAERITSFFAPP--------TVW-----ISLLRHPDF--------------------------- 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 323 DKLVLTKLR-ALLGGRIkmMPcgGAKLEaSIGSFFHSIGI-NIklgYGMTET--TATVSCWQDKGFNPNSIGTLMPNAEV 398
Cdd:PRK08316 281 DTRDLSSLRkGYYGASI--MP--VEVLK-ELRERLPGLRFyNC---YGQTEIapLATVLGPEEHLRRPGSAGRPVLNVET 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 399 KI----------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAP 468
Cdd:PRK08316 353 RVvdddgndvapGEVGEIVHRSPQLMLGYWDDPEKTAEAF-RGGWFHSGDLGVMDEEGYITVVDRKKDMIKTG-GENVAS 430
|
490 500 510
....*....|....*....|....*....|....*.
gi 544685990 469 QYIEGKIGKDKFIEQIAVIA--DAK--KYVSALIVP 500
Cdd:PRK08316 431 REVEEALYTHPAVAEVAVIGlpDPKwiEAVTAVVVP 466
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
183-487 |
3.07e-28 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 115.84 E-value: 3.07e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 183 YTSGTTGEPKGVMLDYANLAHqlNAHDLA--LNVNEDDVSLSFLPLSHIFER--AWIAYVFHrGATNCYLEDTKHVRDAL 258
Cdd:cd05917 9 FTSGTTGSPKGATLTHHNIVN--NGYFIGerLGLTEQDRLCIPVPLFHCFGSvlGVLACLTH-GATMVFPSPSFDPLAVL 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 259 TTL---KPTVMCAVPRFYEKIytavwdkvekaplhrralfnwairvgeqhyqIEQPSQwlrlqyalaDKLVLTKLRallG 335
Cdd:cd05917 86 EAIekeKCTALHGVPTMFIAE-------------------------------LEHPDF---------DKFDLSSLR---T 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 336 GRIKMMPCGGAKLEASIgSFFHSIGINIklGYGMTETTATvsCWQDKGFNP-----NSIGTLMPNAEVKI---------- 400
Cdd:cd05917 123 GIMAGAPCPPELMKRVI-EVMNMKDVTI--AYGMTETSPV--STQTRTDDSiekrvNTVGRIMPHTEAKIvdpeggivpp 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDK 479
Cdd:cd05917 198 vGVPGELCIRGYSVMKGYWNDPEKTAEAIDGDGWLHTGDLAVMDEDGYCRIVGRIKD-MIIRGGENIYPREIEEFLHTHP 276
|
....*...
gi 544685990 480 FIEQIAVI 487
Cdd:cd05917 277 KVSDVQVV 284
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
172-454 |
6.27e-28 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 119.64 E-value: 6.27e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 172 SKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFE---RAW------IAYVFHRG 242
Cdd:PRK08633 778 TFKPDDTATIIFSSGSEGEPKGVMLSHHNILSNIEQISDVFNLRNDDVILSSLPFFHSFGltvTLWlpllegIKVVYHPD 857
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 243 ATncyleDTKHVRDALTTLKPTVMCAVPRFYeKIYTavwdKVEKAplhrralfnwairvgeqhyqieqpsqwlrlqyala 322
Cdd:PRK08633 858 PT-----DALGIAKLVAKHRATILLGTPTFL-RLYL----RNKKL----------------------------------- 892
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 323 DKLVLTKLRallggrikMMPCGGAKLEASIG-SFFHSIGINIKLGYGMTETTATVSC----------WQDKGFNPNSIGT 391
Cdd:PRK08633 893 HPLMFASLR--------LVVAGAEKLKPEVAdAFEEKFGIRILEGYGATETSPVASVnlpdvlaadfKRQTGSKEGSVGM 964
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990 392 LMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLR---TGDVGEMDSCGNLFITDRL 454
Cdd:PRK08633 965 PLPGVAVRIvdpetfeelppGEDGLILIGGPQVMKGYLGDPEKTAEVIKDIDGIGwyvTGDKGHLDEDGFLTITDRY 1041
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
16-487 |
9.31e-28 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 117.09 E-value: 9.31e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 16 AKKWLNRTALRFREQA-QWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATN 94
Cdd:PRK08008 17 ADVYGHKTALIFESSGgVVRRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARL 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 95 TAKQVEYIVNDADIKILFVgdQEQFDQVY-QIANNCPQLMKIVAMkANMHLRDLPNACYWEDFLNVVPNEAEFEKRLNSk 173
Cdd:PRK08008 97 LREESAWILQNSQASLLVT--SAQFYPMYrQIQQEDATPLRHICL-TRVALPADDGVSSFTQLKAQQPATLCYAPPLST- 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 174 qlSDLFTLIYTSGTTGEPKGVMLDYANL--AHQLNAHDLALnvNEDDVSLSFLPLSHI-FERAWIAYVFHRGATNCYLED 250
Cdd:PRK08008 173 --DDTAEILFTSGTTSRPKGVVITHYNLrfAGYYSAWQCAL--RDDDVYLTVMPAFHIdCQCTAAMAAFSAGATFVLLEK 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 251 tkhvrdalttlkptvmcavprfyekiYTA--VWDKVEKaplhRRALFNWAIRVGEQHYQIEQPSQWLRlQYALADKLVLt 328
Cdd:PRK08008 249 --------------------------YSAraFWGQVCK----YRATITECIPMMIRTLMVQPPSANDR-QHCLREVMFY- 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 klrallggrikmMPCGGAKLEASIGSFfhsiGINIKLGYGMTETtaTVSCWQDKGFNPN---SIGTLMPNAEVKIGEEN- 404
Cdd:PRK08008 297 ------------LNLSDQEKDAFEERF----GVRLLTSYGMTET--IVGIIGDRPGDKRrwpSIGRPGFCYEAEIRDDHn 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 ---------EILVR---GGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIE 472
Cdd:PRK08008 359 rplpageigEICIKgvpGKTIFKEYYLDPKATAKVLEADGWLHTGDTGYVDEEGFFYFVDRRCNMIKRG-GENVSCVELE 437
|
490
....*....|....*
gi 544685990 473 GKIGKDKFIEQIAVI 487
Cdd:PRK08008 438 NIIATHPKIQDIVVV 452
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
177-487 |
1.01e-27 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 114.14 E-value: 1.01e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIF--ERAWIAYVFhRGATNCYLE--DTK 252
Cdd:cd17638 1 DVSDIMFTSGTTGRSKGVMCAHRQTLRAAAAWADCADLTEDDRYLIINPFFHTFgyKAGIVACLL-TGATVVPVAvfDVD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 253 HVRDALTTLKPTVMCAVPRFYEKIytavwdkvekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADKLVLTKLRA 332
Cdd:cd17638 80 AILEAIERERITVLPGPPTLFQSL----------------------------------------LDHPGRKKFDLSSLRA 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 333 LLggrikmmpCGGAKLEASIGSFFHS-IGI-NIKLGYGMTETTATVSCWQDKGFN--PNSIGTLMPNAEVKIGEENEILV 408
Cdd:cd17638 120 AV--------TGAATVPVELVRRMRSeLGFeTVLTAYGLTEAGVATMCRPGDDAEtvATTCGRACPGFEVRIADDGEVLV 191
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 544685990 409 RGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:cd17638 192 RGYNVMQGYLDDPEATAEAIDADGWLHTGDVGELDERGYLRITDRLKD-MYIVGGFNVYPAEVEGALAEHPGVAQVAVI 269
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
21-491 |
1.45e-27 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 116.22 E-value: 1.45e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:PRK03640 17 DRTAIEFEEK----KVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREELL 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFVGD--QEQFDQVYQIanncpqlmkivamkanmhlrdlpnacYWEDFLNVVPNEAEFEkrlNSKQLSDL 178
Cdd:PRK03640 93 WQLDDAEVKCLITDDdfEAKLIPGISV--------------------------KFAELMNGPKEEAEIQ---EEFDLDEV 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 179 FTLIYTSGTTGEPKGVMLDYANlaHQLNAHDLALN--VNEDDVSLSFLPLSHI-----FERAWIaYvfhrGATnCYLE-- 249
Cdd:PRK03640 144 ATIMYTSGTTGKPKGVIQTYGN--HWWSAVGSALNlgLTEDDCWLAAVPIFHIsglsiLMRSVI-Y----GMR-VVLVek 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 -DTKHVRDALTTLKPTVMCAVPRFYEKIytavwdkvekaplhrralfnwAIRVGEQHYqieqPSqwlrlqyaladklvlt 328
Cdd:PRK03640 216 fDAEKINKLLQTGGVTIISVVSTMLQRL---------------------LERLGEGTY----PS---------------- 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 KLRA-LLGGRikmmPCGGAKLEASigsffHSIGINIKLGYGMTETTATVsCWQDKGFNPNSIGT----LMPnAEVKI--- 400
Cdd:PRK03640 255 SFRCmLLGGG----PAPKPLLEQC-----KEKGIPVYQSYGMTETASQI-VTLSPEDALTKLGSagkpLFP-CELKIekd 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 ------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEGK 474
Cdd:PRK03640 324 gvvvppFEEGEIVVKGPNVTKGYLNREDATRETF-QDGWFKTGDIGYLDEEGFLYVLDRRSDLI-ISGGENIYPAEIEEV 401
|
490
....*....|....*....
gi 544685990 475 IGKDKFIEQIAVI--ADAK 491
Cdd:PRK03640 402 LLSHPGVAEAGVVgvPDDK 420
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
9-544 |
1.12e-26 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 114.20 E-value: 1.12e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 9 VNRFRLQAKKWLNRTAlRFREQAQW----QEMSWQTFQQEIDRF-SYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQA 83
Cdd:PRK08751 21 LEQFRTVAEVFATSVA-KFADRPAYhsfgKTITYREADQLVEQFaAYLLGELQLKKGDRVALMMPNCLQYPIATFGVLRA 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 84 RAVAVPIYATNTAKQVEYIVNDADIKILFVGDQeqF-DQVYQIANNCPqlMKIVAMKANMHLRDLPNACYWEDFLNVV-- 160
Cdd:PRK08751 100 GLTVVNVNPLYTPRELKHQLIDSGASVLVVIDN--FgTTVQQVIADTP--VKQVITTGLGDMLGFPKAALVNFVVKYVkk 175
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 161 -------PNEAEFEK--------RLNSKQLS--DLFTLIYTSGTTGEPKGVMLDYANL-AHQLNAHDLALNVNE----DD 218
Cdd:PRK08751 176 lvpeyriNGAIRFREalalgrkhSMPTLQIEpdDIAFLQYTGGTTGVAKGAMLTHRNLvANMQQAHQWLAGTGKleegCE 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 219 VSLSFLPLSHIFERAWIAYVFHR-GATNCYLEDTKHVRDALTTLKPTvmcavpRFyeKIYTAVwdkvekaplhrRALFNW 297
Cdd:PRK08751 256 VVITALPLYHIFALTANGLVFMKiGGCNHLISNPRDMPGFVKELKKT------RF--TAFTGV-----------NTLFNG 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 298 AirvgeqhyqieqpsqwlrLQYALADKLVLTKLRALLGGrikmmpcgGAKLEASIGSFFHSI-GINIKLGYGMTETT--A 374
Cdd:PRK08751 317 L------------------LNTPGFDQIDFSSLKMTLGG--------GMAVQRSVAERWKQVtGLTLVEAYGLTETSpaA 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 375 TVSCWQDKGFNpNSIGTLMPNAEV----------KIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDS 444
Cdd:PRK08751 371 CINPLTLKEYN-GSIGLPIPSTDAcikddagtvlAIGEIGELCIKGPQVMKGYWKRPEETAKVMDADGWLHTGDIARMDE 449
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 445 CGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVIA--DAK--KYVSALIV---PCFDSLEeyakqlnIKYQ 517
Cdd:PRK08751 450 QGFVYIVDRKKDMILVS-GFNVYPNEIEDVIAMMPGVLEVAAVGvpDEKsgEIVKVVIVkkdPALTAED-------VKAH 521
|
570 580
....*....|....*....|....*..
gi 544685990 518 DRIELIKHSdiiqmfERRIHELQKELP 544
Cdd:PRK08751 522 ARANLTGYK------QPRIIEFRKELP 542
|
|
| PRK12582 |
PRK12582 |
acyl-CoA synthetase; Provisional |
17-591 |
1.70e-26 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237144 [Multi-domain] Cd Length: 624 Bit Score: 114.37 E-value: 1.70e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 17 KKWLNRtalRFREQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI---YA- 92
Cdd:PRK12582 65 RPWLAQ---REPGHGQWRKVTYGEAKRAVDALAQALLDLGLDPGRPVMILSGNSIEHALMTLAAMQAGVPAAPVspaYSl 141
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 93 --TNTAKqVEYIVNDADIKILFVGDQEQFDQVYQIAN-NCPQLMKIVAM---KANMHLRDLPnacywedflnVVPNEAEF 166
Cdd:PRK12582 142 msHDHAK-LKHLFDLVKPRVVFAQSGAPFARALAALDlLDVTVVHVTGPgegIASIAFADLA----------ATPPTAAV 210
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 167 EKRLNSKQLSDLFTLIYTSGTTGEPKGV-----MLdYANLAHQLNAHDLALNvNEDDVSLSFLPLSHIFE-RAWIAYVFH 240
Cdd:PRK12582 211 AAAIAAITPDTVAKYLFTSGSTGMPKAVintqrMM-CANIAMQEQLRPREPD-PPPPVSLDWMPWNHTMGgNANFNGLLW 288
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 241 RGATnCYLEDTKHVRD-------ALTTLKPTVMCAVPrfyeKIYTAVWDKVEKAPLHRRALFNwaiRVGEQHYQIEQPSQ 313
Cdd:PRK12582 289 GGGT-LYIDDGKPLPGmfeetirNLREISPTVYGNVP----AGYAMLAEAMEKDDALRRSFFK---NLRLMAYGGATLSD 360
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 314 --WLRLQyALADKLVltklrallGGRIKMMPcggakleasigsffhsiginiklGYGMTET--TATVSCWQDKgfNPNSI 389
Cdd:PRK12582 361 dlYERMQ-ALAVRTT--------GHRIPFYT-----------------------GYGATETapTTTGTHWDTE--RVGLI 406
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 390 GTLMPNAEVK---IGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVG----EMDSCGNLFITDRLKELMKTSN 462
Cdd:PRK12582 407 GLPLPGVELKlapVGDKYEVRVKGPNVTPGYHKDPELTAAAFDEEGFYRLGDAArfvdPDDPEKGLIFDGRVAEDFKLST 486
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 463 GKYIAPqyieGKIGKDKFIEQIAVIADA------KKYVSALIVPCFDSLEEYAKQLNIKYQDrieLIKHSDIIQMFERRI 536
Cdd:PRK12582 487 GTWVSV----GTLRPDAVAACSPVIHDAvvagqdRAFIGLLAWPNPAACRQLAGDPDAAPED---VVKHPAVLAILREGL 559
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 537 HELQKELP-SFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMYNE 591
Cdd:PRK12582 560 SAHNAEAGgSSSRIARALLMTEPPSIDAGEITDKGYINQRAVLERRAALVERLYAE 615
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
177-472 |
1.84e-26 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 113.54 E-value: 1.84e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANL----AHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHR-GATNCYLE-- 249
Cdd:PLN02246 180 DVVALPYSSGTTGLPKGVMLTHKGLvtsvAQQVDGENPNLYFHSDDVILCVLPMFHIYSLNSVLLCGLRvGAAILIMPkf 259
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 DTKHVRDALTTLKPTVMCAVPrfyeKIYTAVwdkvEKAPLhrralfnwairvgeqhyqieqpsqwlrlqyalADKLVLTK 329
Cdd:PLN02246 260 EIGALLELIQRHKVTIAPFVP----PIVLAI----AKSPV--------------------------------VEKYDLSS 299
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 330 LRALLGGRIKMmpcgGAKLEASIGSFFHsigiNIKL--GYGMTETTATVS-CWqdkGF-------NPNSIGTLMPNAEVK 399
Cdd:PLN02246 300 IRMVLSGAAPL----GKELEDAFRAKLP----NAVLgqGYGMTEAGPVLAmCL---AFakepfpvKSGSCGTVVRNAELK 368
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 400 I-----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAP 468
Cdd:PLN02246 369 IvdpetgaslprNQPGEICIRGPQIMKGYLNDPEATANTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKY-KGFQVAP 447
|
....
gi 544685990 469 QYIE 472
Cdd:PLN02246 448 AELE 451
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
21-472 |
3.35e-26 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 112.72 E-value: 3.35e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRF--REQAQWQEMSWQTFQQEIDRFSYALiaQHIDIQ-DKIGIFANNMPRWTIADFGAMQARAVAVPIYATN--- 94
Cdd:cd05931 8 DRPAYTFldDEGGREETLTYAELDRRARAIAARL--QAVGKPgDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLPPPTpgr 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 95 TAKQVEYIVNDADIKILFVgDQEQFDQVYQIANNCPQlmkivamkanmhlrDLPNACYWEDFLNVVPNEAEfekRLNSKQ 174
Cdd:cd05931 86 HAERLAAILADAGPRVVLT-TAAALAAVRAFAASRPA--------------AGTPRLLVVDLLPDTSAADW---PPPSPD 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 175 LSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSH----IFerAWIAYVFHrGATNCYLED 250
Cdd:cd05931 148 PDDIAYLQYTSGSTGTPKGVVVTHRNLLANVRQIRRAYGLDPGDVVVSWLPLYHdmglIG--GLLTPLYS-GGPSVLMSP 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 251 TKHVRD------ALTTLKPTVMcAVPRFyekiytavwdkvekaplhrrAlFNWAIRvgeqhyqieqpsqwlRLQYALADK 324
Cdd:cd05931 225 AAFLRRplrwlrLISRYRATIS-AAPNF--------------------A-YDLCVR---------------RVRDEDLEG 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 325 LVLTKLRALLGG--RI----------KMMPCGgAKLEAsigsffhsiginIKLGYGMTETTATVSC-WQDKG-----FNP 386
Cdd:cd05931 268 LDLSSWRVALNGaePVrpatlrrfaeAFAPFG-FRPEA------------FRPSYGLAEATLFVSGgPPGTGpvvlrVDR 334
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 387 N--------------------SIGTLMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEETAKAF------T 429
Cdd:cd05931 335 DalagravavaaddpaarelvSCGRPLPDQEVRIvdpetgrelpdGEVGEIWVRGPSVASGYWGRPEATAETFgalaatD 414
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 544685990 430 EDGFLRTGDVGEM-DscGNLFITDRLKELMkTSNGKYIAPQYIE 472
Cdd:cd05931 415 EGGWLRTGDLGFLhD--GELYITGRLKDLI-IVRGRNHYPQDIE 455
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
35-501 |
7.30e-26 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 111.62 E-value: 7.30e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 35 EMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVg 114
Cdd:PRK06188 37 RLTYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLMAIGAAQLAGLRRTALHPLGSLDDHAYVLEDAGISTLIV- 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 115 DQEQF-DQVYQIANNCPQLMKIVAMKANMHLRDLPNACywedflnvvpneAEFE-KRLNSKQLS-DLFTLIYTSGTTGEP 191
Cdd:PRK06188 116 DPAPFvERALALLARVPSLKHVLTLGPVPDGVDLLAAA------------AKFGpAPLVAAALPpDIAGLAYTGGTTGKP 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 192 KGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIfERAWIAYVFHRGATncyledtkhvrdalttlkpTVMCavPR 271
Cdd:PRK06188 184 KGVMGTHRSIATMAQIQLAEWEWPADPRFLMCTPLSHA-GGAFFLPTLLRGGT-------------------VIVL--AK 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 272 FYEKiytAVWDKVEKaplHRralFNWAIRVgeqhyqieqPSQWlrlqYALAD-----KLVLTKLRALLGGRIKMMPcggA 346
Cdd:PRK06188 242 FDPA---EVLRAIEE---QR---ITATFLV---------PTMI----YALLDhpdlrTRDLSSLETVYYGASPMSP---V 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 347 KL-EA--SIGSFFHSIginiklgYGMTETTATVSCWQDKGFNPNSIGTL----MPNA------------EVKIGEENEIL 407
Cdd:PRK06188 297 RLaEAieRFGPIFAQY-------YGQTEAPMVITYLRKRDHDPDDPKRLtscgRPTPglrvalldedgrEVAQGEVGEIC 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 408 VRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:PRK06188 370 VRGPLVMDGYWNRPEETAEAF-RDGWLHTGDVAREDEDGFYYIVDRKKD-MIVTGGFNVFPREVEDVLAEHPAVAQVAVI 447
|
490
....*....|....*...
gi 544685990 488 A--DAK--KYVSALIVPC 501
Cdd:PRK06188 448 GvpDEKwgEAVTAVVVLR 465
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
47-486 |
1.37e-25 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 111.00 E-value: 1.37e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 47 RFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgDQEQFDQVYQIA 126
Cdd:PRK06018 51 KVSQALDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVNPRLFPEQIAWIINHAEDRVVIT-DLTFVPILEKIA 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 127 NNCPQLMKIVAM--KANMHLRDLPNACYWEDFLNVVPNE---AEFEKRLNSkqlsdlfTLIYTSGTTGEPKGVMLDY-AN 200
Cdd:PRK06018 130 DKLPSVERYVVLtdAAHMPQTTLKNAVAYEEWIAEADGDfawKTFDENTAA-------GMCYTSGTTGDPKGVLYSHrSN 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 201 LAHQLNAHDL-ALNVNEDDVSLSFLPLSHifERAW-IAYVFHRGATNCYLE----DTKHVRDALTTLKPTVMCAVPrfye 274
Cdd:PRK06018 203 VLHALMANNGdALGTSAADTMLPVVPLFH--ANSWgIAFSAPSMGTKLVMPgaklDGASVYELLDTEKVTFTAGVP---- 276
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 275 kiytAVWdkvekaplhrralfnwairvgeqhyqieqpsqWLRLQYALADKLVLTKLrallggriKMMPCGGAKLEASIGS 354
Cdd:PRK06018 277 ----TVW--------------------------------LMLLQYMEKEGLKLPHL--------KMVVCGGSAMPRSMIK 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 355 FFHSIGINIKLGYGMTETT--ATVSCWQD----------------KGFNPNSIgtlmpnaEVKI----GEE--------N 404
Cdd:PRK06018 313 AFEDMGVEVRHAWGMTEMSplGTLAALKPpfsklpgdarldvlqkQGYPPFGV-------EMKItddaGKElpwdgktfG 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 EILVRGGMVMRGYYKkpeETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNGKYIAPqyIEgkigkdkfIEQI 484
Cdd:PRK06018 386 RLKVRGPAVAAAYYR---VDGEILDDDGFFDTGDVATIDAYGYMRITDRSKDVIK-SGGEWISS--ID--------LENL 451
|
..
gi 544685990 485 AV 486
Cdd:PRK06018 452 AV 453
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
43-487 |
2.14e-25 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 110.50 E-value: 2.14e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 43 QEIDRFSYALIA--QHIDIQ--DKIGIFANNMPRWTIADFGAMQARAVAV---PIYatnTAKQVEYIVNDADIKILFVgd 115
Cdd:PRK07059 52 GELDELSRALAAwlQSRGLAkgARVAIMMPNVLQYPVAIAAVLRAGYVVVnvnPLY---TPRELEHQLKDSGAEAIVV-- 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 116 QEQF-DQVYQIANNCPQLMKIVAMKANMH----------LR---------DLPNACYWEDFLnvvpnEAEFEKRLNSKQL 175
Cdd:PRK07059 127 LENFaTTVQQVLAKTAVKHVVVASMGDLLgfkghivnfvVRrvkkmvpawSLPGHVRFNDAL-----AEGARQTFKPVKL 201
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 176 --SDLFTLIYTSGTTGEPKGVMLDYAN-LAHQLNA---HDLALNVNEDDVSLSF---LPLSHIFerawiayvfhrGATNC 246
Cdd:PRK07059 202 gpDDVAFLQYTGGTTGVSKGATLLHRNiVANVLQMeawLQPAFEKKPRPDQLNFvcaLPLYHIF-----------ALTVC 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 247 YLEdtkhvrdALTTLKPTVMCAVPRfyeKIYTAVwDKVEKAPLHR----RALFNwAIrvgeqhyqieqpsqwlrLQYALA 322
Cdd:PRK07059 271 GLL-------GMRTGGRNILIPNPR---DIPGFI-KELKKYQVHIfpavNTLYN-AL-----------------LNNPDF 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 323 DKLVLTKLRALLGGrikmmpcGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCwqdkgfNP-------NSIGTLMPN 395
Cdd:PRK07059 322 DKLDFSKLIVANGG-------GMAVQRPVAERWLEMTGCPITEGYGLSETSPVATC------NPvdatefsGTIGLPLPS 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 396 AEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKY 465
Cdd:PRK07059 389 TEVSIrdddgndlplGEPGEICIRGPQVMAGYWNRPDETAKVMTADGFFRTGDVGVMDERGYTKIVDRKKDMILVS-GFN 467
|
490 500
....*....|....*....|..
gi 544685990 466 IAPQYIEGKIGKDKFIEQIAVI 487
Cdd:PRK07059 468 VYPNEIEEVVASHPGVLEVAAV 489
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
37-535 |
5.35e-25 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 107.85 E-value: 5.35e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 37 SWQTFQQEIDRFSYALIAQHIDIQDKIgifANNMPRW---TIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFV 113
Cdd:cd05903 3 TYSELDTRADRLAAGLAALGVGPGDVV---AFQLPNWwefAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVV 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 114 gdQEQFDQvyqianncpqlMKIVAMKAnmhlrdlpnacywedflnvvpneaefekrlnskqlsDLFTLIYTSGTTGEPKG 193
Cdd:cd05903 80 --PERFRQ-----------FDPAAMPD------------------------------------AVALLLFTSGTTGEPKG 110
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 194 VMLDYANL---AHQLNAHdlaLNVNEDDVSLSFLPLSHIferawIAYVFhrGATNCYLEDTkhvrdalttlkPTVMCAVp 270
Cdd:cd05903 111 VMHSHNTLsasIRQYAER---LGLGPGDVFLVASPMAHQ-----TGFVY--GFTLPLLLGA-----------PVVLQDI- 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 rfyekiytavWDKvekaplhrralfNWAIRVGEQHyqieqpsqwlRLQYALADKLVLTKL-RALLGG-----RIKMMPCG 344
Cdd:cd05903 169 ----------WDP------------DKALALMREH----------GVTFMMGATPFLTDLlNAVEEAgeplsRLRTFVCG 216
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 345 GAKLEASIGSFFHSIGINIKLG-YGMTET-TATVSCWQDK-GFNPNSIGTLMPNAEVKI----------GEENEILVRGG 411
Cdd:cd05903 217 GATVPRSLARRAAELLGAKVCSaYGSTECpGAVTSITPAPeDRRLYTDGRPLPGVEIKVvddtgatlapGVEGELLSRGP 296
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 412 MVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEGKIGKDKFIEQIAVIA--D 489
Cdd:cd05903 297 SVFLGYLDRPDLTADAA-PEGWFRTGDLARLDEDGYLRITGRSKDII-IRGGENIPVLEVEDLLLGHPGVIEAAVVAlpD 374
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 490 AK--KYVSALIV------PCFDSLEEY------AKQlniKYQDRIELIKH-----SDIIQMFERR 535
Cdd:cd05903 375 ERlgERACAVVVtksgalLTFDELVAYldrqgvAKQ---YWPERLVHVDDlprtpSGKVQKFRLR 436
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
61-486 |
9.52e-25 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 108.60 E-value: 9.52e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 61 DKIGIFANNMPRWTIADFGAMQARAVAV---PIYA-------------------TNTAKQVEYIVNDADIK--ILF-VGD 115
Cdd:PRK08974 75 DRVALMMPNLLQYPIALFGILRAGMIVVnvnPLYTprelehqlndsgakaivivSNFAHTLEKVVFKTPVKhvILTrMGD 154
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 116 QEQFDQvYQIANNCPQLMKIVAMKanmhlRDLPNACYWEDFLNVVPNEAEFEKRLNSkqlSDLFTLIYTSGTTGEPKGVM 195
Cdd:PRK08974 155 QLSTAK-GTLVNFVVKYIKRLVPK-----YHLPDAISFRSALHKGRRMQYVKPELVP---EDLAFLQYTGGTTGVAKGAM 225
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 196 LDY----ANLAHQLNAHDLALNVNEDDVSLSfLPLSHIFERAWIAYVF-HRGATNCYLEDTKHVRDALTTLKPTVMCAVp 270
Cdd:PRK08974 226 LTHrnmlANLEQAKAAYGPLLHPGKELVVTA-LPLYHIFALTVNCLLFiELGGQNLLITNPRDIPGFVKELKKYPFTAI- 303
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 rfyekiyTAVwdkvekaplhrRALFNWAIRVGEQHyqieqpsqwlrlqyaladKLVLTKLRALLGGrikmmpcgGAKLEA 350
Cdd:PRK08974 304 -------TGV-----------NTLFNALLNNEEFQ------------------ELDFSSLKLSVGG--------GMAVQQ 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 351 SIGSFFHSI-GINIKLGYGMTETTATVSC--WQDKGFNpNSIGTLMPNAEVKI----------GEENEILVRGGMVMRGY 417
Cdd:PRK08974 340 AVAERWVKLtGQYLLEGYGLTECSPLVSVnpYDLDYYS-GSIGLPVPSTEIKLvdddgnevppGEPGELWVKGPQVMLGY 418
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 418 YKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIG-KDKFIEQIAV 486
Cdd:PRK08974 419 WQRPEATDEVI-KDGWLATGDIAVMDEEGFLRIVDRKKDMILVS-GFNVYPNEIEDVVMlHPKVLEVAAV 486
|
|
| PRK08180 |
PRK08180 |
feruloyl-CoA synthase; Reviewed |
181-591 |
1.50e-24 |
|
feruloyl-CoA synthase; Reviewed
Pssm-ID: 236175 [Multi-domain] Cd Length: 614 Bit Score: 108.04 E-value: 1.50e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDD--VSLSFLPLSHIF-ERAWIAYVFHRGATnCYLEDTKHVRDA 257
Cdd:PRK08180 214 FLFTSGSTGLPKAVINTHRMLCANQQMLAQTFPFLAEEppVLVDWLPWNHTFgGNHNLGIVLYNGGT-LYIDDGKPTPGG 292
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 258 L-TTLK------PTVMCAVPRFYEKIYTAvwdkVEKAPLHRRALFNwaiRVGEQHYQIEQPSQ--WLRLQyALAdklvlt 328
Cdd:PRK08180 293 FdETLRnlreisPTVYFNVPKGWEMLVPA----LERDAALRRRFFS---RLKLLFYAGAALSQdvWDRLD-RVA------ 358
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 klRALLGGRIKMMPcggakleasigsffhsiginiklGYGMTET--TATVSCWQDKGfnPNSIGTLMPNAEVKI---GEE 403
Cdd:PRK08180 359 --EATCGERIRMMT-----------------------GLGMTETapSATFTTGPLSR--AGNIGLPAPGCEVKLvpvGGK 411
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 404 NEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGD----VGEMD-SCGNLFitD-RLKELMKTSNGKYIApqyiegkIG- 476
Cdd:PRK08180 412 LEVRVKGPNVTPGYWRAPELTAEAFDEEGYYRSGDavrfVDPADpERGLMF--DgRIAEDFKLSSGTWVS-------VGp 482
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 477 -KDKFIEQIA-VIADA------KKYVSALIVPCFDSLEEYAKQLniKYQDRIELIKHSDIIQMFERRIHELQKELP-SFE 547
Cdd:PRK08180 483 lRARAVSAGApLVQDVvitghdRDEIGLLVFPNLDACRRLAGLL--ADASLAEVLAHPAVRAAFRERLARLNAQATgSST 560
|
410 420 430 440
....*....|....*....|....*....|....*....|....
gi 544685990 548 QVKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMYNE 591
Cdd:PRK08180 561 RVARALLLDEPPSLDAGEITDKGYINQRAVLARRAALVEALYAD 604
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
12-487 |
7.92e-24 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 105.45 E-value: 7.92e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 12 FRLQ-AKKWLNRTAlrFREQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI 90
Cdd:PLN02330 33 FVLQdAELYADKVA--FVEAVTGKAVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGVFSGA 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 91 YATNTAKQVEYIVNDADIKiLFVGDQEQFDQVYQIanncpQLMKIVAMKANmhlrdLPNACYWEDFLNVVPNEAEfeKRL 170
Cdd:PLN02330 111 NPTALESEIKKQAEAAGAK-LIVTNDTNYGKVKGL-----GLPVIVLGEEK-----IEGAVNWKELLEAADRAGD--TSD 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 171 NSKQL-SDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNE--DDVSLSFLPLSHIFERAWIAYVFHRGatncy 247
Cdd:PLN02330 178 NEEILqTDLCALPFSSGTTGISKGVMLTHRNLVANLCSSLFSVGPEMigQVVTLGLIPFFHIYGITGICCATLRN----- 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 248 ledtkhvrdalttlKPTVMcAVPRFYEKIY--TAVWDKVEKAPLHRRALFNWairvgeqhyqIEQPsqwlrlqyaLADKL 325
Cdd:PLN02330 253 --------------KGKVV-VMSRFELRTFlnALITQEVSFAPIVPPIILNL----------VKNP---------IVEEF 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 326 VLTKLRallggrIKMMPCGGAKLEASIGSFFHSI--GINIKLGYGMTETTATVSCWQD--KGF---NPNSIGTLMPNAEV 398
Cdd:PLN02330 299 DLSKLK------LQAIMTAAAPLAPELLTAFEAKfpGVQVQEAYGLTEHSCITLTHGDpeKGHgiaKKNSVGFILPNLEV 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 399 K-IGEEN----------EILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIA 467
Cdd:PLN02330 373 KfIDPDTgrslpkntpgELCVRSQCVMQGYYNNKEETDRTIDEDGWLHTGDIGYIDDDGDIFIVDRIKELIKY-KGFQVA 451
|
490 500
....*....|....*....|
gi 544685990 468 PQYIEGKIGKDKFIEQIAVI 487
Cdd:PLN02330 452 PAELEAILLTHPSVEDAAVV 471
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
11-448 |
9.76e-24 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 106.48 E-value: 9.76e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 11 RFRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFannMPR---WTIADFGAMQARAVA 87
Cdd:COG1020 481 LFEAQAARTPDAVAVVFGDQ----SLTYAELNARANRLAHHLRALGVGPGDLVGVC---LERsleMVVALLAVLKAGAAY 553
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 88 VPIYATNTAKQVEYIVNDADIKILfVGDQEQFDQVYQIAnncpqlMKIVAMkanmhlrDLPNACYWEDFLNVVPNEAEfe 167
Cdd:COG1020 554 VPLDPAYPAERLAYMLEDAGARLV-LTQSALAARLPELG------VPVLAL-------DALALAAEPATNPPVPVTPD-- 617
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 168 krlnskqlsDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSH------IFeRAWIAyvfhr 241
Cdd:COG1020 618 ---------DLAYVIYTSGSTGRPKGVMVEHRALVNLLAWMQRRYGLGPGDRVLQFASLSFdasvweIF-GALLS----- 682
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 242 GATnCYLEDTKHVRDA------LTTLKPTVMCAVPrfyekiytAVWdkvekaplhrRALfnwairvgeqhyqieqpsqwl 315
Cdd:COG1020 683 GAT-LVLAPPEARRDPaalaelLARHRVTVLNLTP--------SLL----------RAL--------------------- 722
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 316 rLQYALADklvLTKLRALLggrikmmpCGGAKLEASIGSFFHSIGINIKL--GYGMTETTATVSCWQ----DKGFNPNSI 389
Cdd:COG1020 723 -LDAAPEA---LPSLRLVL--------VGGEALPPELVRRWRARLPGARLvnLYGPTETTVDSTYYEvtppDADGGSVPI 790
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 390 GTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFL-------RTGDVGEMDSCGNL 448
Cdd:COG1020 791 GRPIANTRVYVldahlqpvpvGVPGELYIGGAGLARGYLNRPELTAERFVADPFGfpgarlyRTGDLARWLPDGNL 866
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
11-472 |
3.54e-23 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 103.88 E-value: 3.54e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 11 RFRLQAKKWLNRTALRF-REQAQWQE---MSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAmQARAV 86
Cdd:PRK07529 30 LLSRAAARHPDAPALSFlLDADPLDRpetWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWGG-EAAGI 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 87 AVPIYATNTAKQVEYIVNDADIKILF-VG---DQEQFDQVYQIANNCPQLMKIVAMKANmhlRDLPNACYWE-------- 154
Cdd:PRK07529 109 ANPINPLLEPEQIAELLRAAGAKVLVtLGpfpGTDIWQKVAEVLAALPELRTVVEVDLA---RYLPGPKRLAvplirrka 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 155 -----DFLNVVPNE----AEFEKRLNSKQLSDLFtliYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLP 225
Cdd:PRK07529 186 harilDFDAELARQpgdrLFSGRPIGPDDVAAYF---HTGGTTGMPKLAQHTHGNEVANAWLGALLLGLGPGDTVFCGLP 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 226 LSHIFErawiAYV-----FHRGATncyledtkhvrdalttlkptVMCAVPRFY--EKIYTAVWDKVEkaplhrralfnwa 298
Cdd:PRK07529 263 LFHVNA----LLVtglapLARGAH--------------------VVLATPQGYrgPGVIANFWKIVE------------- 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 299 irvgeqHYQIEQPSQwlrlqyaladklVLTKLRALL----GGR----IKMMPCGGAKLEASIG-SFFHSIGINIKLGYGM 369
Cdd:PRK07529 306 ------RYRINFLSG------------VPTVYAALLqvpvDGHdissLRYALCGAAPLPVEVFrRFEAATGVRIVEGYGL 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 370 TETTATVSCwqdkgfNP-------NSIGTLMPNAEVKI---------------GEENEILVRGGMVMRGYYKkPEETAKA 427
Cdd:PRK07529 368 TEATCVSSV------NPpdgerriGSVGLRLPYQRVRVvilddagrylrdcavDEVGVLCIAGPNVFSGYLE-AAHNKGL 440
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 544685990 428 FTEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIE 472
Cdd:PRK07529 441 WLEDGWLNTGDLGRIDADGYFWLTGRAKDLI-IRGGHNIDPAAIE 484
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
177-491 |
4.89e-23 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 100.42 E-value: 4.89e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANL---AHQLNahdLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLE--DT 251
Cdd:cd17637 1 DPFVIIHTAAVAGRPRGAVLSHGNLiaaNLQLI---HAMGLTEADVYLNMLPLFHIAGLNLALATFHAGGANVVMEkfDP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 252 KHVRDALTTLKPTVMCAVPrfyeKIYTAVWDKVEKAPLHrralfnwairvgeqhyqieqpsqwlrlqyaladklvLTKLR 331
Cdd:cd17637 78 AEALELIEEEKVTLMGSFP----PILSNLLDAAEKSGVD------------------------------------LSSLR 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 332 ALLGGRikmMPCGGAKLEASIGSFFHSiginiklGYGMTETT--ATVSCWQDKgfnPNSIGTLMPNAEVKI--------- 400
Cdd:cd17637 118 HVLGLD---APETIQRFEETTGATFWS-------LYGQTETSglVTLSPYRER---PGSAGRPGPLVRVRIvddndrpvp 184
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRL--KELMKTSnGKYIAPQYIEGKIGK 477
Cdd:cd17637 185 aGETGEIVVRGPLVFQGYWNLPELTAYTF-RNGWHHTGDLGRFDEDGYLWYAGRKpeKELIKPG-GENVYPAEVEKVILE 262
|
330
....*....|....*.
gi 544685990 478 DKFIEQIAVI--ADAK 491
Cdd:cd17637 263 HPAIAEVCVIgvPDPK 278
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
21-499 |
1.04e-22 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 101.17 E-value: 1.04e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:cd05945 6 DRPAVVEGGR----TLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAERIR 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFV-GDqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqlsDLF 179
Cdd:cd05945 82 EILDAAKPALLIAdGD-------------------------------------------------------------DNA 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 180 TLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS------HIFErAWIAyvfhrGATnCYL---ED 250
Cdd:cd05945 101 YIIFTSGSTGRPKGVQISHDNLVSFTNWMLSDFPLGPGDVFLNQAPFSfdlsvmDLYP-ALAS-----GAT-LVPvprDA 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 251 TKHVRDALTTLKP---TVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFnwairVGEQhyqieqpsqwlrLQYALADKLVl 327
Cdd:cd05945 174 TADPKQLFRFLAEhgiTVWVSTPSFAAMCLLSPTFTPESLPSLRHFLF-----CGEV------------LPHKTARALQ- 235
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 328 tklRALLGGRIkmmpcggakleasigsffhsigINIklgYGMTETTATVSCWQ-----DKGFNPNSIGTLMPNAEVKI-- 400
Cdd:cd05945 236 ---QRFPDARI----------------------YNT---YGPTEATVAVTYIEvtpevLDGYDRLPIGYAKPGAKLVIld 287
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 --------GEENEILVRGGMVMRGYYKKPEETAKAFTED---GFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQ 469
Cdd:cd05945 288 edgrpvppGEKGELVISGPSVSKGYLNNPEKTAAAFFPDegqRAYRTGDLVRLEADGLLFYRGRLDFQVKL-NGYRIELE 366
|
490 500 510
....*....|....*....|....*....|.
gi 544685990 470 YIEGKIGKDKFIEQIAVIADAKK-YVSALIV 499
Cdd:cd05945 367 EIEAALRQVPGVKEAVVVPKYKGeKVTELIA 397
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
61-491 |
1.18e-22 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 101.42 E-value: 1.18e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 61 DKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILfVGDQeqfdqvyQIANNCPQLMKIVAMKA 140
Cdd:PRK09088 48 ERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDALLQDAEPRLL-LGDD-------AVAAGRTDVEDLAAFIA 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 141 NmhlrdlpnacywedflnVVPNEAEFEKRLNSKQLSdlfTLIYTSGTTGEPKGVMLDYANLAHqlNAHDLALNVNEDDVS 220
Cdd:PRK09088 120 S-----------------ADALEPADTPSIPPERVS---LILFTSGTSGQPKGVMLSERNLQQ--TAHNFGVLGRVDAHS 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 221 lSFLPLSHIFerawiayvfhrgatncyledtkHVRDALTTLKPTVMCA----VPRFYEKIYTAVWdkvekapLHRRALfn 296
Cdd:PRK09088 178 -SFLCDAPMF----------------------HIIGLITSVRPVLAVGgsilVSNGFEPKRTLGR-------LGDPAL-- 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 297 wairvGEQHY-QIEQPSQWLRLQYALaDKLVLTKLRALLGGrikmmpcGGAKLEASIGSFFHSiGINIKLGYGMTETTAT 375
Cdd:PRK09088 226 -----GITHYfCVPQMAQAFRAQPGF-DAAALRHLTALFTG-------GAPHAAEDILGWLDD-GIPMVDGFGMSEAGTV 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 376 VSCWQDKGFNPN---SIGTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEM 442
Cdd:PRK09088 292 FGMSVDCDVIRAkagAAGIPTPTVQTRVvddqgndcpaGVPGELLLRGPNLSPGYWRRPQATARAFTGDGWFRTGDIARR 371
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|.
gi 544685990 443 DSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDKFIEQIAVI--ADAK 491
Cdd:PRK09088 372 DADGFFWVVDRKKD-MFISGGENVYPAEIEAVLADHPGIRECAVVgmADAQ 421
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
20-491 |
1.55e-22 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 101.27 E-value: 1.55e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 20 LNRTALRFRE-------QAQWqemSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYA 92
Cdd:PRK07470 13 LRQAARRFPDrialvwgDRSW---TWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTNF 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 93 TNTAKQVEYIVNDADIKiLFVGDQEQFDQVYQIANNCPQLMKIVAMKAnmhlrdlpnACYWEDFLNVVPNEAEFEKRLNS 172
Cdd:PRK07470 90 RQTPDEVAYLAEASGAR-AMICHADFPEHAAAVRAASPDLTHVVAIGG---------ARAGLDYEALVARHLGARVANAA 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 173 KQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAH--DLALNVNEDDVSLSFLPLSH---IFERAWIAyvfhRGATNCY 247
Cdd:PRK07470 160 VDHDDPCWFFFTSGTTGRPKAAVLTHGQMAFVITNHlaDLMPGTTEQDASLVVAPLSHgagIHQLCQVA----RGAATVL 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 248 LEDTKHVRDALTTL----KPTVMCAVPrfyekiyTAVWDKVEKAPLHRR--ALFNWAIRVGEQHYQIEQpsqwlrlQYAL 321
Cdd:PRK07470 236 LPSERFDPAEVWALverhRVTNLFTVP-------TILKMLVEHPAVDRYdhSSLRYVIYAGAPMYRADQ-------KRAL 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 322 AdKL--VLTKLRAL--LGGRIKMMPcggakleasigSFFHSI--GINIKLGY-GMTETTATVSCWQDKGfnpnsigtlmp 394
Cdd:PRK07470 302 A-KLgkVLVQYFGLgeVTGNITVLP-----------PALHDAedGPDARIGTcGFERTGMEVQIQDDEG----------- 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 395 nAEVKIGEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGK 474
Cdd:PRK07470 359 -RELPPGETGEICVIGPAVFAGYYNNPEANAKAF-RDGWFRTGDLGHLDARGFLYITGRASD-MYISGGSNVYPREIEEK 435
|
490
....*....|....*....
gi 544685990 475 IGKDKFIEQIAV--IADAK 491
Cdd:PRK07470 436 LLTHPAVSEVAVlgVPDPV 454
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
177-457 |
2.77e-22 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 100.82 E-value: 2.77e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIferAWIAYVfhrgatncyledtkHVRD 256
Cdd:cd05906 168 DLALLMLTSGSTGFPKAVPLTHRNILARSAGKIQHNGLTPQDVFLNWVPLDHV---GGLVEL--------------HLRA 230
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 257 ALTtlkptvMCavprfyEKIYTAVwdkvekaplhrralfnwairvgeqHYQIEQPSQWLRL------QYALADKLVLTKL 330
Cdd:cd05906 231 VYL------GC------QQVHVPT------------------------EEILADPLRWLDLidryrvTITWAPNFAFALL 274
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 331 RALLG---------GRIKMMPCGGAKLEASIGSFFHSI----GIN---IKLGYGMTETTA----TVSCWQDKGFNPN--- 387
Cdd:cd05906 275 NDLLEeiedgtwdlSSLRYLVNAGEAVVAKTIRRLLRLlepyGLPpdaIRPAFGMTETCSgviySRSFPTYDHSQALefv 354
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 388 SIGTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDScGNLFITDRLKEL 457
Cdd:cd05906 355 SLGRPIPGVSMRIvddegqllpeGEVGRLQVRGPVVTKGYYNNPEANAEAFTEDGWFRTGDLGFLDN-GNLTITGRTKDT 433
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
21-575 |
4.05e-22 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 99.67 E-value: 4.05e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:cd12116 2 DATAVRDDDR----SLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLR 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILfVGDQEQFDQVyqiANNCPQLMKIVAMKANMHLRDLPNacywedflnVVPNeaefekrlnskqlsDLFT 180
Cdd:cd12116 78 YILEDAEPALV-LTDDALPDRL---PAGLPVLLLALAAAAAAPAAPRTP---------VSPD--------------DLAY 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLS-------------FLPLSHiferawiayvfhrGATnCY 247
Cdd:cd12116 131 VIYTSGSTGRPKGVVVSHRNLVNFLHSMRERLGLGPGDRLLAvttyafdisllelLLPLLA-------------GAR-VV 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 248 LEDTKHVRDA------LTTLKPTVMCAVPrfyekiytAVWdkvekaplhrRALFNwairvgeqhyqieqpSQWLRLQyal 321
Cdd:cd12116 197 IAPRETQRDPealarlIEAHSITVMQATP--------ATW----------RMLLD---------------AGWQGRA--- 240
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 322 adklvltKLRALlggrikmmpCGGAKLEASIGSFFHSIG---INIklgYGMTETTaTVSCWQ--DKGFNPNSIGTLMPNA 396
Cdd:cd12116 241 -------GLTAL---------CGGEALPPDLAARLLSRVgslWNL---YGPTETT-IWSTAArvTAAAGPIPIGRPLANT 300
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 397 E----------VKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFL-------RTGDVGEMDSCGNLFITDRLKELMK 459
Cdd:cd12116 301 QvyvldaalrpVPPGVPGELYIGGDGVAQGYLGRPALTAERFVPDPFAgpgsrlyRTGDLVRRRADGRLEYLGRADGQVK 380
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 460 TsNGKYIAPQYIEGKIGKDKFIEQIAVIA---DAKKYVSALIVPCFDSLeeyakqlnikyQDRIELIKHsdiiqmferri 536
Cdd:cd12116 381 I-RGHRIELGEIEAALAAHPGVAQAAVVVredGGDRRLVAYVVLKAGAA-----------PDAAALRAH----------- 437
|
570 580 590 600
....*....|....*....|....*....|....*....|.
gi 544685990 537 heLQKELPSFeqvkrftLLPQAFsTAMEEI--TPTLKLRRK 575
Cdd:cd12116 438 --LRATLPAY-------MVPSAF-VRLDALplTANGKLDRK 468
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
85-580 |
4.10e-22 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 100.26 E-value: 4.10e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 85 AVAVPIYATNTAKQVEYIVNDADIKILFVGDQEQFDQVYQIAN-NCPQLMKIVAMKAnmhlrDLPNAcyWEDFLNVVPNE 163
Cdd:cd05970 97 AIAIPATHQLTAKDIVYRIESADIKMIVAIAEDNIPEEIEKAApECPSKPKLVWVGD-----PVPEG--WIDFRKLIKNA 169
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 164 AEFEKR--LNSKQLSDLFTLIY-TSGTTGEPKGVMLDYA-NLAHQLNAHdLALNVNEDDVSLSflplshIFERAWIAYVF 239
Cdd:cd05970 170 SPDFERptANSYPCGEDILLVYfSSGTTGMPKMVEHDFTyPLGHIVTAK-YWQNVREGGLHLT------VADTGWGKAVW 242
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 240 HR------GATNCYLED-----TKHVRDALTTLKPTVMCAVPRFYekiytavwdkvekaplhrRALfnwaIRVGEQHYQI 308
Cdd:cd05970 243 GKiygqwiAGAAVFVYDydkfdPKALLEKLSKYGVTTFCAPPTIY------------------RFL----IREDLSRYDL 300
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 309 EQpsqwlrLQYALAdklvltklrallggrikmmpCGGAKLEASIGSFFHSIGINIKLGYGMTETT---ATVSCWQDKgfn 385
Cdd:cd05970 301 SS------LRYCTT--------------------AGEALNPEVFNTFKEKTGIKLMEGFGQTETTltiATFPWMEPK--- 351
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 386 PNSIGTLMPN----------AEVKIGEENEILVRG------GMvMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLF 449
Cdd:cd05970 352 PGSMGKPAPGyeidlidregRSCEAGEEGEIVIRTskgkpvGL-FGGYYKDAEKTAEVW-HDGYYHTGDAAWMDEDGYLW 429
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 450 ITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAV--IADAKK--YVSALIVpcfdsleeyakqLNIKYQDRIELIKH 525
Cdd:cd05970 430 FVGRTDDLIKSS-GYRIGPFEVESALIQHPAVLECAVtgVPDPIRgqVVKATIV------------LAKGYEPSEELKKE 496
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 526 sdiIQMFERRIHELQKELPSFEQVKRftlLPQAFSTameeitptlKLRRKVIMQR 580
Cdd:cd05970 497 ---LQDHVKKVTAPYKYPRIVEFVDE---LPKTISG---------KIRRVEIRER 536
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
47-488 |
6.92e-22 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 99.44 E-value: 6.92e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 47 RFSYALIAQHIDIQDKIgifANNMPRW---TIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQEQ----F 119
Cdd:PRK06087 61 RLANWLLAKGIEPGDRV---AFQLPGWcefTIIYLACLKVGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTLFKqtrpV 137
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 120 DQVYQIANNCPQLMKIVAM-KANMHLRDLPNACYWEDF--LNVVPNEAEfekrlnskqlSDLFTLIYTSGTTGEPKGVML 196
Cdd:PRK06087 138 DLILPLQNQLPQLQQIVGVdKLAPATSSLSLSQIIADYepLTTAITTHG----------DELAAVLFTSGTEGLPKGVML 207
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 197 DYANLAHQLNAHDLALNVNEDDVSLSFLPLSHiferawiAYVFHRGATNCYLEDTKHV-------RDALTTL---KPT-V 265
Cdd:PRK06087 208 THNNILASERAYCARLNLTWQDVFMMPAPLGH-------ATGFLHGVTAPFLIGARSVlldiftpDACLALLeqqRCTcM 280
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 266 MCAVPRFYEKIYTavwdkVEKAPLHrralfnwairvgeqhyqieqpsqwlrlqyaladklvLTKLRALLggrikmmpCGG 345
Cdd:PRK06087 281 LGATPFIYDLLNL-----LEKQPAD------------------------------------LSALRFFL--------CGG 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 346 AKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDK--GFNPNSIGTLMPNAEVKI----------GEENEILVRGGMV 413
Cdd:PRK06087 312 TTIPKKVARECQQRGIKLLSVYGSTESSPHAVVNLDDplSRFMHTDGYAAAGVEIKVvdearktlppGCEGEEASRGPNV 391
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 414 MRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:PRK06087 392 FMGYLDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDII-VRGGENISSREVEDILLQHPKIHDACVVA 465
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
50-500 |
1.15e-21 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 98.41 E-value: 1.15e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 50 YALIAQHIDIQDKIGIFANNMPRWTIADFGAMQAR----------------AV-------AVPIY-AT----------NT 95
Cdd:PRK07514 6 FDALRAAFADRDAPFIETPDGLRYTYGDLDAASARlanllvalgvkpgdrvAVqvekspeALALYlATlragavflplNT 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 96 AKQ---VEYIVNDADIKiLFVGDQEQFDQVYQIANNC--PQLMKIVA------MKANMHLRDlpnacyweDFlNVVPNEA 164
Cdd:PRK07514 86 AYTlaeLDYFIGDAEPA-LVVCDPANFAWLSKIAAAAgaPHVETLDAdgtgslLEAAAAAPD--------DF-ETVPRGA 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 165 EfekrlnskqlsDLFTLIYTSGTTGEPKGVMLDYANLAHqlNAhdLALN----VNEDDVSLSFLPlshiferawiayVFH 240
Cdd:PRK07514 156 D-----------DLAAILYTSGTTGRSKGAMLSHGNLLS--NA--LTLVdywrFTPDDVLIHALP------------IFH 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 241 -RG---ATNCYLE-----------DTKHVRDALTtlKPTVMCAVPRFYekiytavwdkvekaplhrralfnwairvgeqh 305
Cdd:PRK07514 209 tHGlfvATNVALLagasmiflpkfDPDAVLALMP--RATVMMGVPTFY-------------------------------- 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 306 yqieqpsqwLRLqyaLADKlVLTKLRAllgGRIKMMPCGGAKLEASI-GSFFHSIGINIKLGYGMTETTATVScwqdkgf 384
Cdd:PRK07514 255 ---------TRL---LQEP-RLTREAA---AHMRLFISGSAPLLAEThREFQERTGHAILERYGMTETNMNTS------- 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 385 NP-------NSIGTLMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCG 446
Cdd:PRK07514 312 NPydgerraGTVGFPLPGVSLRVtdpetgaelppGEIGMIEVKGPNVFKGYWRMPEKTAEEFRADGFFITGDLGKIDERG 391
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 544685990 447 NLFITDRLKELMkTSNGKYIAPQYIEGkigkdkFIEQI------AVI----ADAKKYVSALIVP 500
Cdd:PRK07514 392 YVHIVGRGKDLI-ISGGYNVYPKEVEG------EIDELpgvvesAVIgvphPDFGEGVTAVVVP 448
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
11-575 |
4.24e-21 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 96.63 E-value: 4.24e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 11 RFRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI 90
Cdd:cd17655 2 LFEEQAEKTPDHTAVVFEDQ----TLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPI 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 91 YATNTAKQVEYIVNDADIKILFVgdQEQFDQvyqianncpqlmKIVAMKANMHLRDLPNACYWEDFLNVVPNEaefekrl 170
Cdd:cd17655 78 DPDYPEERIQYILEDSGADILLT--QSHLQP------------PIAFIGLIDLLDEDTIYHEESENLEPVSKS------- 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 171 nskqlSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLShiFErAWIAYVFHR---GATNC- 246
Cdd:cd17655 137 -----DDLAYVIYTSGSTGKPKGVMIEHRGVVNLVEWANKVIYQGEHLRVALFASIS--FD-ASVTEIFASllsGNTLYi 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 247 YLEDTKHVRDALTtlkptvmcavpRFYEKIYTAVwdkVEKAPLHrralfnwairvgeqhyqieqpsqwlrLQyaladklV 326
Cdd:cd17655 209 VRKETVLDGQALT-----------QYIRQNRITI---IDLTPAH--------------------------LK-------L 241
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 327 LTKLRALLGGRIKMMPCGGAKLEAS-IGSFFHSIGINIKL--GYGMTETTATVSCWQ-----DKGFNPnSIGTLMPNAEV 398
Cdd:cd17655 242 LDAADDSEGLSLKHLIVGGEALSTElAKKIIELFGTNPTItnAYGPTETTVDASIYQyepetDQQVSV-PIGKPLGNTRI 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 399 KI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFL------RTGDVGEMDSCGNLFITDRLKELMKTsN 462
Cdd:cd17655 321 YIldqygrpqpvGVAGELYIGGEGVARGYLNRPELTAEKFVDDPFVpgermyRTGDLARWLPDGNIEFLGRIDHQVKI-R 399
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 463 GKYIAPQYIEGKIGKDKFIEQIAVIA----DAKKYVSALIVpcFDsleeyakqlnikyqdrieliKHSDIIQMFERrihe 538
Cdd:cd17655 400 GYRIELGEIEARLLQHPDIKEAVVIArkdeQGQNYLCAYIV--SE--------------------KELPVAQLREF---- 453
|
570 580 590
....*....|....*....|....*....|....*....
gi 544685990 539 LQKELPSFeqvkrftLLPqAFSTAMEEI--TPTLKLRRK 575
Cdd:cd17655 454 LARELPDY-------MIP-SYFIKLDEIplTPNGKVDRK 484
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
173-472 |
4.87e-21 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 96.79 E-value: 4.87e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 173 KQLSDLFTLI-YTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHifERAWIAyvFH-----RGATNC 246
Cdd:cd05908 102 CELADELAFIqFSSGSTGDPKGVMLTHENLVHNMFAILNSTEWKTKDRILSWMPLTH--DMGLIA--FHlapliAGMNQY 177
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 247 YLEDTKHVRDALTTL------KPTVMCAvPRFYEKIYTAVWdKVEKAP----LHRRALFNWAirvgeqhyqieQPsqwlr 316
Cdd:cd05908 178 LMPTRLFIRRPILWLkkasehKATIVSS-PNFGYKYFLKTL-KPEKANdwdlSSIRMILNGA-----------EP----- 239
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 317 LQYALADKLvLTKLRALLGGRIKMMPCGGAKlEASIGSFFHSIGINIK----------LGYGMTETTATVScwqdKGFNP 386
Cdd:cd05908 240 IDYELCHEF-LDHMSKYGLKRNAILPVYGLA-EASVGASLPKAQSPFKtitlgrrhvtHGEPEPEVDKKDS----ECLTF 313
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 387 NSIGTLMPNAEVKI-GEENEIL---------VRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDScGNLFITDRLKE 456
Cdd:cd05908 314 VEVGKPIDETDIRIcDEDNKILpdgyighiqIRGKNVTPGYYNNPEATAKVFTDDGWLKTGDLGFIRN-GRLVITGREKD 392
|
330
....*....|....*.
gi 544685990 457 LMKTsNGKYIAPQYIE 472
Cdd:cd05908 393 IIFV-NGQNVYPHDIE 407
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
22-472 |
6.95e-21 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 96.43 E-value: 6.95e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 22 RTALRFREQAQWQEMSWQTFQQEIDRFSYALIA---QHIDIQ--DKIGIFANNMPRWTIADFGAMQARAVAV---PIYAT 93
Cdd:PRK12492 32 RSCKKFADRPAFSNLGVTLSYAELERHSAAFAAylqQHTDLVpgDRIAVQMPNVLQYPIAVFGALRAGLIVVntnPLYTA 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 94 NT-------------------AKQVEYIVNDADIKILF---VGDQEQFDQVYQIANNCPQLMKIVAmkaNMHLrdlPNAC 151
Cdd:PRK12492 112 REmrhqfkdsgaralvylnmfGKLVQEVLPDTGIEYLIeakMGDLLPAAKGWLVNTVVDKVKKMVP---AYHL---PQAV 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 152 yweDFLNVVPNEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLA----------HQLNAHDLALNVNEDDVSL 221
Cdd:PRK12492 186 ---PFKQALRQGRGLSLKPVPVGLDDIAVLQYTGGTTGLAKGAMLTHGNLVanmlqvraclSQLGPDGQPLMKEGQEVMI 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 222 SFLPLSHIferawiaYVFhrgATNCYLedtkhvrdALTTLKPTVMCAVPRfyekiytavwdkveKAPLHRRALFNWairv 301
Cdd:PRK12492 263 APLPLYHI-------YAF---TANCMC--------MMVSGNHNVLITNPR--------------DIPGFIKELGKW---- 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 302 geqhyqieQPSQWLRLQYALADKLVLTKLRALLGGRIKMMPCGGAKLEASIGSFFHSI-GINIKLGYGMTETTATVSCwq 380
Cdd:PRK12492 307 --------RFSALLGLNTLFVALMDHPGFKDLDFSALKLTNSGGTALVKATAERWEQLtGCTIVEGYGLTETSPVAST-- 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 381 dkgfNP-------NSIGTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMD 443
Cdd:PRK12492 377 ----NPygelarlGTVGIPVPGTALKVidddgnelplGERGELCIKGPQVMKGYWQQPEATAEALDAEGWFKTGDIAVID 452
|
490 500
....*....|....*....|....*....
gi 544685990 444 SCGNLFITDRLKELMKTSnGKYIAPQYIE 472
Cdd:PRK12492 453 PDGFVRIVDRKKDLIIVS-GFNVYPNEIE 480
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
7-491 |
3.70e-20 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 94.07 E-value: 3.70e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 7 HFVNRFRLQAKKWLNRTALRFREQAqwqeMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAV 86
Cdd:PRK07786 18 NWVNQLARHALMQPDAPALRFLGNT----TTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAI 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 87 AVPIYATNTAKQVEYIVNDADIKILFVGDQEQfDQVYQIANNCPQLMKIVAMKANMHLRDLPnacyWEDFLNV------- 159
Cdd:PRK07786 94 AVPVNFRLTPPEIAFLVSDCGAHVVVTEAALA-PVATAVRDIVPLLSTVVVAGGSSDDSVLG----YEDLLAEagpahap 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 160 --VPNEAEfekrlnskqlsdlfTLI-YTSGTTGEPKGVMLDYANLAHQLNAHDLALNVN-EDDVSLSFLPLSHIFERAWI 235
Cdd:PRK07786 169 vdIPNDSP--------------ALImYTSGTTGRPKGAVLTHANLTGQAMTCLRTNGADiNSDVGFVGVPLFHIAGIGSM 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 236 AYVFHRGATNCYLE----DTKHVRDALTTLKPTVMCAVPrfyekiytAVWDKVEKAPLHR-RALfnwAIRVgeqhyqieq 310
Cdd:PRK07786 235 LPGLLLGAPTVIYPlgafDPGQLLDVLEAEKVTGIFLVP--------AQWQAVCAEQQARpRDL---ALRV--------- 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 311 psqwLRLQYALADKLVLTKLRAllggrikMMPcggaklEASIGSFFhsiginiklgyGMTETTAtVSCW---QDKGFNPN 387
Cdd:PRK07786 295 ----LSWGAAPASDTLLRQMAA-------TFP------EAQILAAF-----------GQTEMSP-VTCMllgEDAIRKLG 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 388 SIGTLMPNA----------EVKIGEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKEl 457
Cdd:PRK07786 346 SVGKVIPTVaarvvdenmnDVPVGEVGEIVYRAPTLMSGYWNNPEATAEAF-AGGWFHSGDLVRQDEEGYVWVVDRKKD- 423
|
490 500 510
....*....|....*....|....*....|....*.
gi 544685990 458 MKTSNGKYIAPQYIEGKIGKDKFIEQIAVI--ADAK 491
Cdd:PRK07786 424 MIISGGENIYCAEVENVLASHPDIVEVAVIgrADEK 459
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
42-511 |
1.43e-19 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 92.05 E-value: 1.43e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 42 QQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgdQEQFDQ 121
Cdd:cd05959 36 EAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTLLTPDDYAYYLEDSRARVVVV--SGELAP 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 122 VYQIANN--CPQLMKIVAMKANMHLRDLPnacyweDFLNVVPNEAEFEKRLNSKQLSDLFTLiYTSGTTGEPKGVMLDYA 199
Cdd:cd05959 114 VLAAALTksEHTLVVLIVSGGAGPEAGAL------LLAELVAAEAEQLKPAATHADDPAFWL-YSSGSTGRPKGVVHLHA 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 200 NLAHQLN--AHDLaLNVNEDDVSLSFLPLSHiferawiAY--------VFHRGATnCYLE----DTKHVRDALTTLKPTV 265
Cdd:cd05959 187 DIYWTAElyARNV-LGIREDDVCFSAAKLFF-------AYglgnsltfPLSVGAT-TVLMperpTPAAVFKRIRRYRPTV 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 266 MCAVPRFYekiytAVWDKVEKAPlhrralfnwairvgeqhyqieqpsqwlrlQYALAdklvltklrallggRIKMMPCGG 345
Cdd:cd05959 258 FFGVPTLY-----AAMLAAPNLP-----------------------------SRDLS--------------SLRLCVSAG 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 346 AKLEASIGSFFHS-IGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI----------GEENEILVRGGMVM 414
Cdd:cd05959 290 EALPAEVGERWKArFGLDILDGIGSTEMLHIFLSNRPGRVRYGTTGKPVPGYEVELrdedggdvadGEPGELYVRGPSSA 369
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 415 RGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVIA----DA 490
Cdd:cd05959 370 TMYWNNRDKTRDTF-QGEWTRTGDKYVRDDDGFYTYAGRADDMLKVS-GIWVSPFEVESALVQHPAVLEAAVVGvedeDG 447
|
490 500 510
....*....|....*....|....*....|
gi 544685990 491 KKYVSALIVP---------CFDSLEEYAKQ 511
Cdd:cd05959 448 LTKPKAFVVLrpgyedseaLEEELKEFVKD 477
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
46-489 |
1.58e-19 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 92.02 E-value: 1.58e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 46 DRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQEQFDQvyqi 125
Cdd:cd17651 31 NRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYPAERLAFMLADAGPVLVLTHPALAGEL---- 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 126 anncpqlmkivamkanmhLRDLPNACYWEDFLNVVPNEAEFEKRLnskQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQL 205
Cdd:cd17651 107 ------------------AVELVAVTLLDQPGAAAGADAEPDPAL---DADDLAYVIYTSGSTGRPKGVVMPHRSLANLV 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 206 NAHDLALNVNEDDVSLSFLPLSH--IFERAWIAYVFhrGATNCYLEDtkHVRDALTTLkptvmcavprfyekiytavWDK 283
Cdd:cd17651 166 AWQARASSLGPGARTLQFAGLGFdvSVQEIFSTLCA--GATLVLPPE--EVRTDPPAL-------------------AAW 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 284 VEKAPLHRRALFNWAIR-VGEQHYQIEQPSQWLRLQYALADKLVLTK-LRALLGGRikmmpcGGAKLeasigsFFHsigi 361
Cdd:cd17651 223 LDEQRISRVFLPTVALRaLAEHGRPLGVRLAALRYLLTGGEQLVLTEdLREFCAGL------PGLRL------HNH---- 286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 362 niklgYGMTETTaTVSCWQDKGFN-----PNSIGTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAK 426
Cdd:cd17651 287 -----YGPTETH-VVTALSLPGDPaawpaPPPIGRPIDNTRVYVldaalrpvppGVPGELYIGGAGLARGYLNRPELTAE 360
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 544685990 427 AFTEDGFL------RTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGKIGKDKFIEQIAVIAD 489
Cdd:cd17651 361 RFVPDPFVpgarmyRTGDLARWLPDGELEFLGRADDQVKI-RGFRIELGEIEAALARHPGVREAVVLAR 428
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
176-472 |
2.07e-19 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 90.23 E-value: 2.07e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 176 SDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFErawiAYV-----FHRGATncyled 250
Cdd:cd05944 2 DDVAAYFHTGGTTGTPKLAQHTHSNEVYNAWMLALNSLFDPDDVLLCGLPLFHVNG----SVVtlltpLASGAH------ 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 251 tkhvrdalttlkptVMCAVPRFY--EKIYTAVWDKVEkaplhrralfnwairvgeqHYQIEQPSQwlrlqyaladklVLT 328
Cdd:cd05944 72 --------------VVLAGPAGYrnPGLFDNFWKLVE-------------------RYRITSLST------------VPT 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 KLRALLG-------GRIKMMPCGGAKLEASIGSFFH-SIGINIKLGYGMTETTATVSC-WQDKGFNPNSIGTLMPNAEVK 399
Cdd:cd05944 107 VYAALLQvpvnadiSSLRFAMSGAAPLPVELRARFEdATGLPVVEGYGLTEATCLVAVnPPDGPKRPGSVGLRLPYARVR 186
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 400 I---------------GEENEILVRGGMVMRGYYKKpEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGK 464
Cdd:cd05944 187 IkvldgvgrllrdcapDEVGEICVAGPGVFGGYLYT-EGNKNAFVADGWLNTGDLGRLDADGYLFITGRAKDLI-IRGGH 264
|
....*...
gi 544685990 465 YIAPQYIE 472
Cdd:cd05944 265 NIDPALIE 272
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
183-577 |
2.17e-19 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 91.78 E-value: 2.17e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 183 YTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLE--DTKHVRDALTT 260
Cdd:PLN02860 179 FTSGTTGRPKGVTISHSALIVQSLAKIAIVGYGEDDVYLHTAPLCHIGGLSSALAMLMVGACHVLLPkfDAKAALQAIKQ 258
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 261 LKPTVMCAVPrfyekiyTAVWDKVEKAplhrRALFNWAIRvgeqhyqieqpsqwlrlqyaladKLVLTKLRA-------L 333
Cdd:PLN02860 259 HNVTSMITVP-------AMMADLISLT----RKSMTWKVF-----------------------PSVRKILNGggslssrL 304
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 334 LGGRIKMMPCggakleASIGSffhsiginiklGYGMTETTATVS--------------CWQDKGFNPNS---------IG 390
Cdd:PLN02860 305 LPDAKKLFPN------AKLFS-----------AYGMTEACSSLTfmtlhdptlespkqTLQTVNQTKSSsvhqpqgvcVG 367
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 391 TLMPNAEVKIG-----EENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKY 465
Cdd:PLN02860 368 KPAPHVELKIGldessRVGRILTRGPHVMLGYWGQNSETASVLSNDGWLDTGDIGWIDKAGNLWLIGRSNDRIKTG-GEN 446
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 466 IAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFdSLEEYAKQLNIKYQDRieliKHSDIIQMFERRIHELQKELPS 545
Cdd:PLN02860 447 VYPEEVEAVLSQHPGVASVVVVGVPDSRLTEMVVACV-RLRDGWIWSDNEKENA----KKNLTLSSETLRHHCREKNLSR 521
|
410 420 430
....*....|....*....|....*....|..
gi 544685990 546 FEQVKRFTLLPQAFSTameeiTPTLKLRRKVI 577
Cdd:PLN02860 522 FKIPKLFVQWRKPFPL-----TTTGKIRRDEV 548
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
21-472 |
9.50e-19 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 91.00 E-value: 9.50e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRF--REQAQWQEMSWQTFQQEIdRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQ 98
Cdd:PRK05691 24 DRLALRFlaDDPGEGVVLSYRDLDLRA-RTIAAALQARASFGDRAVLLFPSGPDYVAAFFGCLYAGVIAVPAYPPESARR 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 99 -----VEYIVNDADIKILFVGD--QEQFDQVYQI-ANNCPQLMKIvamkanmhlrdlpnacyweDFLNVVPNEAEFEKRL 170
Cdd:PRK05691 103 hhqerLLSIIADAEPRLLLTVAdlRDSLLQMEELaAANAPELLCV-------------------DTLDPALAEAWQEPAL 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 171 nskQLSDLFTLIYTSGTTGEPKGVMLDYANLA--HQLNAHDLALNVNEDDVSLSFLPLSH-----------IFerAWIAY 237
Cdd:PRK05691 164 ---QPDDIAFLQYTSGSTALPKGVQVSHGNLVanEQLIRHGFGIDLNPDDVIVSWLPLYHdmgliggllqpIF--SGVPC 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 238 VFHrgATNCYLEDTKHVRDALTTLKPTVMCAvPRFyekIYTAVWDKVEKAPLHRRALFNWairvgeqhyqieqpsqwlRL 317
Cdd:PRK05691 239 VLM--SPAYFLERPLRWLEAISEYGGTISGG-PDF---AYRLCSERVSESALERLDLSRW------------------RV 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 318 QYALADKLVLTKLRALLGgriKMMPCGgakLEASigSFFHSiginiklgYGMTETTATVSCWQ-----------DKGFNP 386
Cdd:PRK05691 295 AYSGSEPIRQDSLERFAE---KFAACG---FDPD--SFFAS--------YGLAEATLFVSGGRrgqgipaleldAEALAR 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 387 N-----------SIGTLMPNAEVKIGEEN-----------EILVRGGMVMRGYYKKPEETAKAFTE-DG--FLRTGDVGE 441
Cdd:PRK05691 359 NraepgtgsvlmSCGRSQPGHAVLIVDPQslevlgdnrvgEIWASGPSIAHGYWRNPEASAKTFVEhDGrtWLRTGDLGF 438
|
490 500 510
....*....|....*....|....*....|.
gi 544685990 442 MDScGNLFITDRLKElMKTSNGKYIAPQYIE 472
Cdd:PRK05691 439 LRD-GELFVTGRLKD-MLIVRGHNLYPQDIE 467
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
177-488 |
9.66e-19 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 89.90 E-value: 9.66e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANLAHQLNAH-----DLALNVNEDDVSLSFLPLSHIFERAwiayVFHRGatncyledt 251
Cdd:PLN02574 199 DVAAIMYSSGTTGASKGVVLTHRNLIAMVELFvrfeaSQYEYPGSDNVYLAALPMFHIYGLS----LFVVG--------- 265
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 252 khvrdaLTTLKPTVMcAVPRFYEKIYTAVWDkvekaplhrralfnwaiRVGEQHYQIEQPsqwlrlqyaladklVLTKL- 330
Cdd:PLN02574 266 ------LLSLGSTIV-VMRRFDASDMVKVID-----------------RFKVTHFPVVPP--------------ILMALt 307
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 331 ---RALLGG---RIKMMPCGGAKL-EASIGSFFHSIG-INIKLGYGMTETTATVScwqdKGFNP------NSIGTLMPNA 396
Cdd:PLN02574 308 kkaKGVCGEvlkSLKQVSCGAAPLsGKFIQDFVQTLPhVDFIQGYGMTESTAVGT----RGFNTeklskySSVGLLAPNM 383
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 397 EVKI-----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKY 465
Cdd:PLN02574 384 QAKVvdwstgcllppGNCGELWIQGPGVMKGYLNNPKATQSTIDKDGWLRTGDIAYFDEDGYLYIVDRLKEIIKY-KGFQ 462
|
330 340
....*....|....*....|...
gi 544685990 466 IAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:PLN02574 463 IAPADLEAVLISHPEIIDAAVTA 485
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
367-500 |
1.15e-18 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 89.56 E-value: 1.15e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 367 YGMTETTATVSC----WQDKGFNPN-SIGTLMPNAEVKI------------GEENEILVRGGMVMRGYYKKPEETAKAFT 429
Cdd:PRK05852 327 FGMTEATHQVTTtqieGIGQTENPVvSTGLVGRSTGAQIrivgsdglplpaGAVGEVWLRGTTVVRGYLGDPTITAANFT 406
|
90 100 110 120 130 140 150
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 430 eDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVIADAKKY----VSALIVP 500
Cdd:PRK05852 407 -DGWLRTGDLGSLSAAGDLSIRGRIKELINRG-GEKISPERVEGVLASHPNVMEAAVFGVPDQLygeaVAAVIVP 479
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
37-491 |
1.74e-18 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 88.85 E-value: 1.74e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 37 SWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVavpIYATNT---AKQVEYIVNDADIKILFV 113
Cdd:PRK08162 45 TWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAV---LNTLNTrldAASIAFMLRHGEAKVLIV 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 114 gDQEQFDQVYQIANNCPQLMKIV------AMKANMHLRDLPnacyWEDFLNvvPNEAEFEKRLNSKQLsDLFTLIYTSGT 187
Cdd:PRK08162 122 -DTEFAEVAREALALLPGPKPLVidvddpEYPGGRFIGALD----YEAFLA--SGDPDFAWTLPADEW-DAIALNYTSGT 193
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 188 TGEPKGVMldYANLAHQLNA--HDLALNVNEDDVSLSFLPLSHI----FerAWIayVFHRGATNCYLE--DTKHVRDALT 259
Cdd:PRK08162 194 TGNPKGVV--YHHRGAYLNAlsNILAWGMPKHPVYLWTLPMFHCngwcF--PWT--VAARAGTNVCLRkvDPKLIFDLIR 267
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 260 TLKPTVMCAvprfyekiytavwdkvekAPLHRRALFNwairvgeqhyqieQPSQWlrlqyaladklvltklRALLGGRIK 339
Cdd:PRK08162 268 EHGVTHYCG------------------APIVLSALIN-------------APAEW----------------RAGIDHPVH 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 340 MMPCGGAKLEASIGSFfHSIGINIKLGYGMTET--TATVSCWQD-------------KG--------------FNPNSiG 390
Cdd:PRK08162 301 AMVAGAAPPAAVIAKM-EEIGFDLTHVYGLTETygPATVCAWQPewdalplderaqlKArqgvryplqegvtvLDPDT-M 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 391 TLMPNAEVKIGEeneILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQY 470
Cdd:PRK08162 379 QPVPADGETIGE---IMFRGNIVMKGYLKNPKATEEAF-AGGWFHTGDLAVLHPDGYIKIKDRSKDII-ISGGENISSIE 453
|
490 500
....*....|....*....|...
gi 544685990 471 IEGKIGKDKFIEQIAVIA--DAK 491
Cdd:PRK08162 454 VEDVLYRHPAVLVAAVVAkpDPK 476
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
176-488 |
3.19e-18 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 87.39 E-value: 3.19e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 176 SDLFTLIYTSGTTGEPKGVMLDY-ANLAHQLNAHDlALNVNEDDVSLSflplshIFERAWIAYVFHR-------GATN-- 245
Cdd:cd05972 81 EDPALIYFTSGTTGLPKGVLHTHsYPLGHIPTAAY-WLGLRPDDIHWN------IADPGWAKGAWSSffgpwllGATVfv 153
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 246 CYLE--DTKHVRDALTTLKPTVMCAVPrfyekiytAVWDKVEKAPLHRRALfnwairvgeqhyqieqpsqwLRLQYALad 323
Cdd:cd05972 154 YEGPrfDAERILELLERYGVTSFCGPP--------TAYRMLIKQDLSSYKF--------------------SHLRLVV-- 203
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 324 klvltklrallggrikmmpCGGAKL-EASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI-- 400
Cdd:cd05972 204 -------------------SAGEPLnPEVIEWWRAATGLPIRDGYGQTETGLTVGNFPDMPVKPGSMGRPTPGYDVAIid 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 --------GEENEILVRGGMV--MRGYYKKPEETAKAFTEDgFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQY 470
Cdd:cd05972 265 ddgrelppGEEGDIAIKLPPPglFLGYVGDPEKTEASIRGD-YYLTGDRAYRDEDGYFWFVGRADDIIKSS-GYRIGPFE 342
|
330
....*....|....*...
gi 544685990 471 IEGKIGKDKFIEQIAVIA 488
Cdd:cd05972 343 VESALLEHPAVAEAAVVG 360
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
12-500 |
7.38e-18 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 86.45 E-value: 7.38e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 12 FRLQAKKWLNRTALRFREQAqwqeMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:cd17645 4 FEEQVERTPDHVAVVDRGQS----LTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPID 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 92 ATNTAKQVEYIVNDADIKILFvgdqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrln 171
Cdd:cd17645 80 PDYPGERIAYMLADSSAKILL----------------------------------------------------------- 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 172 sKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLShiFErAWIAYVFhrgaTNCYLEDT 251
Cdd:cd17645 101 -TNPDDLAYVIYTSGSTGLPKGVMIEHHNLVNLCEWHRPYFGVTPADKSLVYASFS--FD-ASAWEIF----PHLTAGAA 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 252 KHVRDALTTLKptvMCAVPRFYEKiytavwdkvekaplHRRALFNWAIRVGEQHYQIEQPSqwlrlqyaladklvltkLR 331
Cdd:cd17645 173 LHVVPSERRLD---LDALNDYFNQ--------------EGITISFLPTGAAEQFMQLDNQS-----------------LR 218
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 332 ALLggrikmmpCGGAKLEAsigsfFHSIGINIKLGYGMTETTATVSCWQ-DKGFNPNSIGTLMPNAEVKI---------- 400
Cdd:cd17645 219 VLL--------TGGDKLKK-----IERKGYKLVNNYGPTENTVVATSFEiDKPYANIPIGKPIDNTRVYIldealqlqpi 285
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFL------RTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGK 474
Cdd:cd17645 286 GVAGELCIAGEGLARGYLNRPELTAEKFIVHPFVpgermyRTGDLAKFLPDGNIEFLGRLDQQVKI-RGYRIEPGEIEPF 364
|
490 500 510
....*....|....*....|....*....|
gi 544685990 475 IGKDKFIEQIAVIA----DAKKYVSALIVP 500
Cdd:cd17645 365 LMNHPLIELAAVLAkedaDGRKYLVAYVTA 394
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
43-459 |
3.28e-17 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 84.28 E-value: 3.28e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 43 QEIDRFSYAL---IAQHIDIQ-DKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILfvgdqeq 118
Cdd:cd17653 26 GELDAASNALanrLLQLGVVPgDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAKLPSARIQAILRTSGATLL------- 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 119 fdqvyqIANNCPQlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqlsDLFTLIYTSGTTGEPKGVMLDY 198
Cdd:cd17653 99 ------LTTDSPD---------------------------------------------DLAYIIFTSGSTGIPKGVMVPH 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 199 ANLAHQLNAHDLALNVNEDDVSLSFLPLShiFErAWIAYVFH---RGATNCYLEDTKHVRDALTTLkpTVMCAVPRFYEK 275
Cdd:cd17653 128 RGVLNYVSQPPARLDVGPGSRVAQVLSIA--FD-ACIGEIFStlcNGGTLVLADPSDPFAHVARTV--DALMSTPSILST 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 276 IYTAVWDKVEKaplhrralfnwaIRVGEqhyqiEQPSQWLrlqyaladklvltkLRALLGGRikmmpcggakleasigSF 355
Cdd:cd17653 203 LSPQDFPNLKT------------IFLGG-----EAVPPSL--------------LDRWSPGR----------------RL 235
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 356 FHsiginiklGYGMTETTATVSCWQDKGFNPNSIGTLMPNA----------EVKIGEENEILVRGGMVMRGYYKKPEETA 425
Cdd:cd17653 236 YN--------AYGPTECTISSTMTELLPGQPVTIGKPIPNStcyildadlqPVPEGVVGEICISGVQVARGYLGNPALTA 307
|
410 420 430 440
....*....|....*....|....*....|....*....|
gi 544685990 426 KAFTEDGF------LRTGDVGEMDSCGNLFITDRLKELMK 459
Cdd:cd17653 308 SKFVPDPFwpgsrmYRTGDYGRWTEDGGLEFLGREDNQVK 347
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
182-453 |
7.28e-17 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 83.50 E-value: 7.28e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 182 IYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIferawiayvfH-----------RGATNCYL-- 248
Cdd:PRK07787 134 VYTSGTTGPPKGVVLSRRAIAADLDALAEAWQWTADDVLVHGLPLFHV----------HglvlgvlgplrIGNRFVHTgr 203
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 249 EDTKHVRDALTTlKPTVMCAVPrfyekiytAVWDKVEKAPLHRRALfnwairvgeqhyqieQPSQWLRLQYALADKLVLT 328
Cdd:PRK07787 204 PTPEAYAQALSE-GGTLYFGVP--------TVWSRIAADPEAARAL---------------RGARLLVSGSAALPVPVFD 259
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 KLRALLGGRIkmmpcggakLEAsigsffhsiginiklgYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKIGEEN---- 404
Cdd:PRK07787 260 RLAALTGHRP---------VER----------------YGMTETLITLSTRADGERRPGWVGLPLAGVETRLVDEDggpv 314
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990 405 --------EILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDR 453
Cdd:PRK07787 315 phdgetvgELQVRGPTLFDGYLNRPDATAAAFTADGWFRTGDVAVVDPDGMHRIVGR 371
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
51-472 |
2.33e-16 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 82.06 E-value: 2.33e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 51 ALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFvgdqeqFDQ-----VYQI 125
Cdd:PRK07008 55 ALAALGVEPGDRVGTLAWNGYRHLEAYYGVSGSGAVCHTINPRLFPEQIAYIVNHAEDRYVL------FDLtflplVDAL 128
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 126 ANNCPQLMKIVAM--KANMHLRDLPNACYwEDFLNVVPNEAEFeKRLNSKQLSdlfTLIYTSGTTGEPKGVMldYANLAH 203
Cdd:PRK07008 129 APQCPNVKGWVAMtdAAHLPAGSTPLLCY-ETLVGAQDGDYDW-PRFDENQAS---SLCYTSGTTGNPKGAL--YSHRST 201
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 204 QLNAHDLAL----NVNEDDVSLSFLPLSHIfeRAW-IAYvfhrgatNCYLEDTKHVrdalttlkptvmcavprFYEKiyt 278
Cdd:PRK07008 202 VLHAYGAALpdamGLSARDAVLPVVPMFHV--NAWgLPY-------SAPLTGAKLV-----------------LPGP--- 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 279 avwdKVEKAPLHRraLFNwAIRVGeqhYQIEQPSQWLR-LQYALADKLVLTKLRALLGGrikmmpcGGAKLEASIGSFFH 357
Cdd:PRK07008 253 ----DLDGKSLYE--LIE-AERVT---FSAGVPTVWLGlLNHMREAGLRFSTLRRTVIG-------GSACPPAMIRTFED 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 358 SIGINIKLGYGMTE-----TTATVScWQDKGFNPNSI-------GTLMPNAEVKI----GEE--------NEILVRGGMV 413
Cdd:PRK07008 316 EYGVEVIHAWGMTEmsplgTLCKLK-WKHSQLPLDEQrkllekqGRVIYGVDMKIvgddGRElpwdgkafGDLQVRGPWV 394
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*....
gi 544685990 414 MRGYYKKPEETakafTEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNGKYIAPQYIE 472
Cdd:PRK07008 395 IDRYFRGDASP----LVDGWFPTGDVATIDADGFMQITDRSKDVIK-SGGEWISSIDIE 448
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
11-448 |
3.09e-16 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 81.48 E-value: 3.09e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 11 RFRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI 90
Cdd:cd12117 2 LFEEQAARTPDAVAVVYGDR----SLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPL 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 91 YATNTAKQVEYIVNDADIKILfVGDQEQFDQVyqiANNCPQLMKIVAMKAnmhlRDLPNAcywedflnVVPNEAEfekrl 170
Cdd:cd12117 78 DPELPAERLAFMLADAGAKVL-LTDRSLAGRA---GGLEVAVVIDEALDA----GPAGNP--------AVPVSPD----- 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 171 nskqlsDLFTLIYTSGTTGEPKGVMLDYANLAhqlnahDLALNVN-----EDDVSLSFLPLShiFERA----WIAYVFhr 241
Cdd:cd12117 137 ------DLAYVMYTSGSTGRPKGVAVTHRGVV------RLVKNTNyvtlgPDDRVLQTSPLA--FDAStfeiWGALLN-- 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 242 GATnCYLEDTKHVRD------ALTTLKPTVMCAvprfyekiyTAvwdkvekaplhrrALFNwairvgeqhyqieqpsqwl 315
Cdd:cd12117 201 GAR-LVLAPKGTLLDpdalgaLIAEEGVTVLWL---------TA-------------ALFN------------------- 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 316 rlQYALADKLVLTKLRALL-GG--------RIKMMPCGGAKLeasigsffhsigINiklGYGMTETTaTVSCW---QDKG 383
Cdd:cd12117 239 --QLADEDPECFAGLRELLtGGevvspphvRRVLAACPGLRL------------VN---GYGPTENT-TFTTShvvTELD 300
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 384 FNPNS--IGTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFL------RTGDVGEMDSC 445
Cdd:cd12117 301 EVAGSipIGRPIANTRVYVldedgrpvppGVPGELYVGGDGLALGYLNRPALTAERFVADPFGpgerlyRTGDLARWLPD 380
|
...
gi 544685990 446 GNL 448
Cdd:cd12117 381 GRL 383
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
21-488 |
3.48e-16 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 81.99 E-value: 3.48e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:PLN03102 29 NRTSIIYGKT----RFTWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIA 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFVgdQEQFDQVYQianncPQLMKIVAMKANMHLRdlpnacywedflNVVPNEAEFEKRLNSKQLS---- 176
Cdd:PLN03102 105 AILRHAKPKILFV--DRSFEPLAR-----EVLHLLSSEDSNLNLP------------VIFIHEIDFPKRPSSEELDyecl 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 ---------------------DLFTLIYTSGTTGEPKGVMLDyanlahqlnahdlalnvneddvslsflplshiferawi 235
Cdd:PLN03102 166 iqrgeptpslvarmfriqdehDPISLNYTSGTTADPKGVVIS-------------------------------------- 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 236 ayvfHRGAtncYLEDTKHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEkaplhrRALFNWAIRvgeqHYQIEQPSQWL 315
Cdd:PLN03102 208 ----HRGA---YLSTLSAIIGWEMGTCPVYLWTLPMFHCNGWTFTWGTAA------RGGTSVCMR----HVTAPEIYKNI 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 316 RLQYALADKLVLTKLRALL-GGRIKMMP--------CGGAKLEASIGSFFHSIGINIKLGYGMTETTATV-SC-WQD--- 381
Cdd:PLN03102 271 EMHNVTHMCCVPTVFNILLkGNSLDLSPrsgpvhvlTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVlFCeWQDewn 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 382 ----------KGFNPNSIGTLMpNAEVKIGEE-----------NEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVG 440
Cdd:PLN03102 351 rlpenqqmelKARQGVSILGLA-DVDVKNKETqesvprdgktmGEIVIKGSSIMKGYLKNPKATSEAF-KHGWLNTGDVG 428
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 544685990 441 EMDSCGNLFITDRLKELMkTSNGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:PLN03102 429 VIHPDGHVEIKDRSKDII-ISGGENISSVEVENVLYKYPKVLETAVVA 475
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
23-500 |
2.65e-15 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 78.94 E-value: 2.65e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 23 TALRfREQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYI 102
Cdd:PRK13295 44 TAVR-LGTGAPRRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAVLNPLMPIFRERELSFM 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 103 VNDADIKILFVG------DQEQFdqVYQIANNCPQLMKIVAMKAnmhlrDLPNAcyWEDFLNvvpnEAEFEKRLNSKQLS 176
Cdd:PRK13295 123 LKHAESKVLVVPktfrgfDHAAM--ARRLRPELPALRHVVVVGG-----DGADS--FEALLI----TPAWEQEPDAPAIL 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 --------DLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFErawiayvFHRGAtncyl 248
Cdd:PRK13295 190 arlrpgpdDVTQLIYTSGTTGEPKGVMHTANTLMANIVPYAERLGLGADDVILMASPMAHQTG-------FMYGL----- 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 249 edtkhvrdalttLKPTVMCAvprfyEKIYTAVWDKVEKAPLHRRALFNWAIRvgeqhyqiEQPsqwlrlqyALADKLVLT 328
Cdd:PRK13295 258 ------------MMPVMLGA-----TAVLQDIWDPARAAELIRTEGVTFTMA--------STP--------FLTDLTRAV 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 KLRALLGGRIKMMPCGGAKLE-ASIGSFFHSIGINIKLGYGMTETTA-TVSCWQD---KGFNpnSIGTLMPNAEVKI--- 400
Cdd:PRK13295 305 KESGRPVSSLRTFLCAGAPIPgALVERARAALGAKIVSAWGMTENGAvTLTKLDDpdeRAST--TDGCPLPGVEVRVvda 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -------GEENEILVRGGMVMRGYYKKPEETAKAFteDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEG 473
Cdd:PRK13295 383 dgaplpaGQIGRLQVRGCSNFGGYLKRPQLNGTDA--DGWFDTGDLARIDADGYIRISGRSKDVI-IRGGENIPVVEIEA 459
|
490 500 510
....*....|....*....|....*....|.
gi 544685990 474 KIGKDKFIEQIAVIA--DAK--KYVSALIVP 500
Cdd:PRK13295 460 LLYRHPAIAQVAIVAypDERlgERACAFVVP 490
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
36-472 |
3.20e-15 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 78.59 E-value: 3.20e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 36 MSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGD 115
Cdd:PRK12406 12 RSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLIAHA 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 116 qeqfDQVYQIANNCPQLMKIV----------AMKANMHLRDLP-NACYWEDFLnvvpneAEFEKrLNSKQLSDLFTLIYT 184
Cdd:PRK12406 92 ----DLLHGLASALPAGVTVLsvptppeiaaAYRISPALLTPPaGAIDWEGWL------AQQEP-YDGPPVPQPQSMIYT 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 185 SGTTGEPKGVMLDYANLAHQLNAHD---LALNVNEDDVSLSFLPLSHiferawiayvfhrGATNCYledtkhvrdALTTL 261
Cdd:PRK12406 161 SGTTGHPKGVRRAAPTPEQAAAAEQmraLIYGLKPGIRALLTGPLYH-------------SAPNAY---------GLRAG 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 262 K-PTVMCAVPRFYEKiytAVWDKVEKAplhrralfnwaiRVGEQHYQIEQPSQWLRLQYALADKLVLTKLRALLGGrikM 340
Cdd:PRK12406 219 RlGGVLVLQPRFDPE---ELLQLIERH------------RITHMHMVPTMFIRLLKLPEEVRAKYDVSSLRHVIHA---A 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 341 MPCGGAKLEASIGSFfhsiGINIKLGYGMTETTATVSCWQDKGFN-PNSIGTLMPNAEVKI----------GEENEILVR 409
Cdd:PRK12406 281 APCPADVKRAMIEWW----GPVIYEYYGSTESGAVTFATSEDALShPGTVGKAAPGAELRFvdedgrplpqGEIGEIYSR 356
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 544685990 410 -GGMVMRGYYKKPEETAKAfTEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIE 472
Cdd:PRK12406 357 iAGNPDFTYHNKPEKRAEI-DRGGFITSGDVGYLDADGYLFLCDRKRD-MVISGGVNIYPAEIE 418
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
397-525 |
5.11e-15 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 77.75 E-value: 5.11e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 397 EVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEGKIG 476
Cdd:cd05920 329 PVPPGEEGELLTRGPYTIRGYYRAPEHNARAFTPDGFYRTGDLVRRTPDGYLVVEGRIKDQI-NRGGEKIAAEEVENLLL 407
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 544685990 477 KDKFIEQIAVIADAKKY----VSALIV-----PCFDSLEEYAKQLNI---KYQDRIELIKH 525
Cdd:cd05920 408 RHPAVHDAAVVAMPDELlgerSCAFVVlrdppPSAAQLRRFLRERGLaayKLPDRIEFVDS 468
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
13-488 |
5.56e-15 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 77.88 E-value: 5.56e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 13 RLQAKKWLNRTALRFREQaqwqemsWQTFQQEIDRFS---YALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVP 89
Cdd:PRK06155 28 ARQAERYPDRPLLVFGGT-------RWTYAEAARAAAaaaHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVP 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 90 IyatNTA---KQVEYIVNDADIKiLFVGDQEQFDQVYQIANNCPQLMKIVAMkanmhlrDLPNACYWEDFLNVVPNEAEF 166
Cdd:PRK06155 101 I---NTAlrgPQLEHILRNSGAR-LLVVEAALLAALEAADPGDLPLPAVWLL-------DAPASVSVPAGWSTAPLPPLD 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 167 EK-RLNSKQLSDLFTLIYTSGTTGEPKGVMLDYA-------NLAHQLNahdlalnVNEDDVSLSFLPLshiferawiayv 238
Cdd:PRK06155 170 APaPAAAVQPGDTAAILYTSGTTGPSKGVCCPHAqfywwgrNSAEDLE-------IGADDVLYTTLPL------------ 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 239 FHRGATNCYLEdtkhvrdALttLKPTVMCAVPRFYEKIYtavWDKVEKAplhrRALFNWAIrvGEQ-HYQIEQPSqwlrl 317
Cdd:PRK06155 231 FHTNALNAFFQ-------AL--LAGATYVLEPRFSASGF---WPAVRRH----GATVTYLL--GAMvSILLSQPA----- 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 318 qyALADKLvlTKLRALLGgrikmmPCGGAKLEASigsFFHSIGINIKLGYGMTETTATVSC-WQDKgfNPNSIGTLMPNA 396
Cdd:PRK06155 288 --RESDRA--HRVRVALG------PGVPAALHAA---FRERFGVDLLDGYGSTETNFVIAVtHGSQ--RPGSMGRLAPGF 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 397 EVKIGEEN----------EILVRG---GMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNG 463
Cdd:PRK06155 353 EARVVDEHdqelpdgepgELLLRAdepFAFATGYFGMPEKTVEAW-RNLWFHTGDRVVRDADGWFRFVDRIKDAIR-RRG 430
|
490 500
....*....|....*....|....*
gi 544685990 464 KYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:PRK06155 431 ENISSFEVEQVLLSHPAVAAAAVFP 455
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
11-439 |
6.73e-15 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 78.85 E-value: 6.73e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 11 RFRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI 90
Cdd:PRK12316 4556 LVAERARMTPDAVAVVFDEE----KLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGAYVPL 4631
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 91 YATNTAKQVEYIVNDADIKILFVgdQEQFDQVYQIAnncpqlmkivamkANMHLRDLPNACYWEDFlnvvpNEAEFEKRL 170
Cdd:PRK12316 4632 DPEYPRERLAYMMEDSGAALLLT--QSHLLQRLPIP-------------DGLASLALDRDEDWEGF-----PAHDPAVRL 4691
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 171 NSKQLSdlfTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS-HIFERAWIaYVFHRGATncyle 249
Cdd:PRK12316 4692 HPDNLA---YVIYTSGSTGRPKGVAVSHGSLVNHLHATGERYELTPDDRVLQFMSFSfDGSHEGLY-HPLINGAS----- 4762
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 dtKHVRDAlttlkptvmcavprfyekiytAVWDkvekaPLHRRALfnwairVGEQHYQIEQ--PSQWLRL-QYALADKLV 326
Cdd:PRK12316 4763 --VVIRDD---------------------SLWD-----PERLYAE------IHEHRVTVLVfpPVYLQQLaEHAERDGEP 4808
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 327 LTKLRALLGGRikmmPCGGAKLEASIGSFFHSIGINiklGYGMTETTATVSCWQDKGFNPNS-----IGTLMPN------ 395
Cdd:PRK12316 4809 PSLRVYCFGGE----AVAQASYDLAWRALKPVYLFN---GYGPTETTVTVLLWKARDGDACGaaympIGTPLGNrsgyvl 4881
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 396 ----AEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGDV 439
Cdd:PRK12316 4882 dgqlNPLPVGVAGELYLGGEGVARGYLERPALTAERFVPDPFgapggrlYRTGDL 4936
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
21-195 |
8.24e-15 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 77.23 E-value: 8.24e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:PRK07798 18 DRVALVCGDR----RLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRYVEDELR 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFVGDqeQF-DQVYQIANNCPQLMKIVAMKANMHLRDLPNACYWEDFLNVVPNEAEFEKRlnskqlS--D 177
Cdd:PRK07798 94 YLLDDSDAVALVYER--EFaPRVAEVLPRLPKLRTLVVVEDGSGNDLLPGAVDYEDALAAGSPERDFGER------SpdD 165
|
170
....*....|....*...
gi 544685990 178 LFtLIYTSGTTGEPKGVM 195
Cdd:PRK07798 166 LY-LLYTGGTTGMPKGVM 182
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
397-488 |
1.44e-14 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 76.72 E-value: 1.44e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 397 EVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMktsN--GKYIAPQYIEGK 474
Cdd:COG1021 374 PVPPGEVGELLTRGPYTIRGYYRAPEHNARAFTPDGFYRTGDLVRRTPDGYLVVEGRAKDQI---NrgGEKIAAEEVENL 450
|
90
....*....|....
gi 544685990 475 IGKDKFIEQIAVIA 488
Cdd:COG1021 451 LLAHPAVHDAAVVA 464
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
182-488 |
1.46e-14 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 76.19 E-value: 1.46e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 182 IYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDV-----SLSF--------LPLSHIFERAWIAYVFHRGATNCYL 248
Cdd:cd17643 99 IYTSGSTGRPKGVVVSHANVLALFAATQRWFGFNEDDVwtlfhSYAFdfsvweiwGALLHGGRLVVVPYEVARSPEDFAR 178
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 249 EdtkhVRDAlttlKPTVMCAVPrfyekiyTAVwdkvekaplhrRALFNWAIRVGEQHyqieqpsqwLRLQYaladkLVLt 328
Cdd:cd17643 179 L----LRDE----GVTVLNQTP-------SAF-----------YQLVEAADRDGRDP---------LALRY-----VIF- 217
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 klrallggrikmmpcGGAKLE-ASIGSFFHSIG------INiklGYGMTETTATVScW-----QD-KGFNPNSIGTLMPN 395
Cdd:cd17643 218 ---------------GGEALEaAMLRPWAGRFGldrpqlVN---MYGITETTVHVT-FrpldaADlPAAAASPIGRPLPG 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 396 ----------AEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGDVGEMDSCGNLFITDRLKELM 458
Cdd:cd17643 279 lrvyvldadgRPVPPGVVGELYVSGAGVARGYLGRPELTAERFVANPFggpgsrmYRTGDLARRLPDGELEYLGRADEQV 358
|
330 340 350
....*....|....*....|....*....|
gi 544685990 459 KTsNGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:cd17643 359 KI-RGFRIELGEIEAALATHPSVRDAAVIV 387
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
34-488 |
3.90e-14 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 74.81 E-value: 3.90e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 34 QEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFV 113
Cdd:cd05919 9 RSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCEARLVVT 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 114 GDQEqfdqvyqianncpqlmkivamkanmhlrdlpnACYWedflnvvpneaefekrlnskqlsdlftlIYTSGTTGEPKG 193
Cdd:cd05919 89 SADD--------------------------------IAYL----------------------------LYSSGTTGPPKG 108
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 194 VMLDYAN--LAHQLNAHDLaLNVNEDDVSLSflpLSHIFeRAW-----IAYVFHRGATnCYLEDTKHVRDA----LTTLK 262
Cdd:cd05919 109 VMHAHRDplLFADAMAREA-LGLTPGDRVFS---SAKMF-FGYglgnsLWFPLAVGAS-AVLNPGWPTAERvlatLARFR 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 263 PTVMCAVPRFYekiytavwdkvekaplhRRALfnwAIRVGEQHyqieqpsqwlrlqyALADklvltklrallggrIKMMP 342
Cdd:cd05919 183 PTVLYGVPTFY-----------------ANLL---DSCAGSPD--------------ALRS--------------LRLCV 214
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 343 CGGAKLEASIGSFF-HSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI----------GEENEILVRGG 411
Cdd:cd05919 215 SAGEALPRGLGERWmEHFGGPILDGIGATEVGHIFLSNRPGAWRLGSTGRPVPGYEIRLvdeeghtippGEEGDLLVRGP 294
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990 412 MVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:cd05919 295 SAAVGYWNNPEKSRATF-NGGWYRTGDKFCRDADGWYTHAGRADDMLKVG-GQWVSPVEVESLIIQHPAVAEAAVVA 369
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
178-491 |
5.42e-14 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 74.78 E-value: 5.42e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 178 LFTliyTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLEdtkhVRDA 257
Cdd:PRK06164 186 LFT---TSGTTSGPKLVLHRQATLLRHARAIARAYGYDPGAVLLAALPFCGVFGFSTLLGALAGGAPLVCEP----VFDA 258
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 258 LTTLKptvmcavprfyekiytavwdkvekaplhrralfnwaiRVGEQhyqieqpsqwlRLQYALADKLVLTKLRALLGGR 337
Cdd:PRK06164 259 ARTAR-------------------------------------ALRRH-----------RVTHTFGNDEMLRRILDTAGER 290
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 338 ikmMPCGGAKLeASIGSF---FHSI-------GINIKLGYGMTETTATVSCWQ---DKGFNPNSIGTLM-PNAEVKI--- 400
Cdd:PRK06164 291 ---ADFPSARL-FGFASFapaLGELaalararGVPLTGLYGSSEVQALVALQPatdPVSVRIEGGGRPAsPEARVRArdp 366
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 --------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIE 472
Cdd:PRK06164 367 qdgallpdGESGEIEIRAPSLMRGYLDNPDATARALTDDGYFRTGDLGYTRGDGQFVYQTRMGDSLRLG-GFLVNPAEIE 445
|
330
....*....|....*....
gi 544685990 473 gkigkdKFIEQIAVIADAK 491
Cdd:PRK06164 446 ------HALEALPGVAAAQ 458
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
181-488 |
6.60e-14 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 73.94 E-value: 6.60e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLShiFErawiayvfhrGATNCYLedtkhvrdaltt 260
Cdd:cd17649 99 VIYTSGSTGTPKGVAVSHGPLAAHCQATAERYGLTPGDRELQFASFN--FD----------GAHEQLL------------ 154
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 261 lkPTVMCA---VPRFYEkiytaVWDKVEKaplHRRALFNWAIRVGEQhyqieQPSQWlrlqYALADKLVLTKLRAllGGR 337
Cdd:cd17649 155 --PPLICGacvVLRPDE-----LWASADE---LAEMVRELGVTVLDL-----PPAYL----QQLAEEADRTGDGR--PPS 213
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 338 IKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQ---DKGFNPNS--IGTLMPN----------AEVKIGE 402
Cdd:cd17649 214 LRLYIFGGEALSPELLRRWLKAPVRLFNAYGPTEATVTPLVWKceaGAARAGASmpIGRPLGGrsayildadlNPVPVGV 293
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 403 ENEILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGKI 475
Cdd:cd17649 294 TGELYIGGEGLARGYLGRPELTAERFVPDPFgapgsrlYRTGDLARWRDDGVIEYLGRVDHQVKI-RGFRIELGEIEAAL 372
|
330
....*....|...
gi 544685990 476 GKDKFIEQIAVIA 488
Cdd:cd17649 373 LEHPGVREAAVVA 385
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
12-438 |
3.12e-13 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 73.27 E-value: 3.12e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 12 FRLQAKKWLNRTALRFREQAqwqeMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:PRK12467 518 IEAQARQHPERPALVFGEQV----LSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLD 593
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 92 ATNTAKQVEYIVNDADIKILfVGDQEQFDQVYqianncpqlmkiVAMKANMHLRDLPnacywEDFLNVVPnEAEFEKRLN 171
Cdd:PRK12467 594 PEYPQDRLAYMLDDSGVRLL-LTQSHLLAQLP------------VPAGLRSLCLDEP-----ADLLCGYS-GHNPEVALD 654
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 172 SKQLSdlfTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATnCYLEDT 251
Cdd:PRK12467 655 PDNLA---YVIYTSGSTGQPKGVAISHGALANYVCVIAERLQLAADDSMLMVSTFAFDLGVTELFGALASGAT-LHLLPP 730
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 252 KHVRDAlttlkptvmcavPRFYEKIYTAVWDKVEKAPLHRRALFnwairvgeqhyQIEQPSQWLRLQYALadklvltklr 331
Cdd:PRK12467 731 DCARDA------------EAFAALMADQGVTVLKIVPSHLQALL-----------QASRVALPRPQRALV---------- 777
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 332 allggrikmmpCGGAKLEASIGSFFHSIGINIKL--GYGMTETTATVSCW----QDKGFNPNSIGTLMPNAEVKI----- 400
Cdd:PRK12467 778 -----------CGGEALQVDLLARVRALGPGARLinHYGPTETTVGVSTYelsdEERDFGNVPIGQPLANLGLYIldhyl 846
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -----GEENEILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGD 438
Cdd:PRK12467 847 npvpvGVVGELYIGGAGLARGYHRRPALTAERFVPDPFgadggrlYRTGD 896
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
174-500 |
9.90e-13 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 70.57 E-value: 9.90e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 174 QLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDV------SLSFLPLSHIFERAWIA----YVFHRGA 243
Cdd:cd17650 91 QPEDLAYVIYTSGTTGKPKGVMVEHRNVAHAAHAWRREYELDSFPVrllqmaSFSFDVFAGDFARSLLNggtlVICPDEV 170
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 244 tncyLEDTKHVRDALTTLKPTVMCAVPrfyekiytavwdkvekaplhrralfnwAIRVGEQHYQIEQpsqwlrlqyalad 323
Cdd:cd17650 171 ----KLDPAALYDLILKSRITLMESTP---------------------------ALIRPVMAYVYRN------------- 206
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 324 KLVLTKLRALLGGRIKMMPCGGAKLEASIGSffHSIGINiklGYGMTETTATVSCWQ-------DKGFNPnsIGTLMPNA 396
Cdd:cd17650 207 GLDLSAMRLLIVGSDGCKAQDFKTLAARFGQ--GMRIIN---SYGVTEATIDSTYYEegrdplgDSANVP--IGRPLPNT 279
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 397 E----------VKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGF------LRTGDVGEMDSCGNLFITDRLKELMKT 460
Cdd:cd17650 280 AmyvlderlqpQPVGVAGELYIGGAGVARGYLNRPELTAERFVENPFapgermYRTGDLARWRADGNVELLGRVDHQVKI 359
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 544685990 461 sNGKYIAPQYIEGKIGKDKFIEQIAVIA--DAK--KYVSALIVP 500
Cdd:cd17650 360 -RGFRIELGEIESQLARHPAIDEAVVAVreDKGgeARLCAYVVA 402
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
1-493 |
2.73e-12 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 69.27 E-value: 2.73e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 1 MASLDFHFVNRFRLQAKKWLNRTALRFREQAQwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGA 80
Cdd:PRK05857 9 MPQLPSTVLDRVFEQARQQPEAIALRRCDGTS--ALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLAC 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 81 MQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQEQFDqvyqiANNCPQ-LMKIVAMKANMHLRDLPNACYWE-DFLN 158
Cdd:PRK05857 87 AKLGAIAVMADGNLPIAAIERFCQITDPAAALVAPGSKMA-----SSAVPEaLHSIPVIAVDIAAVTRESEHSLDaASLA 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 159 VVPNEAEfekrlnskqlSDLFTLIYTSGTTGEPKGVML---DYANLAHQLNAHDLA-LNVNEDDVSLSFLPLSHIFERAW 234
Cdd:PRK05857 162 GNADQGS----------EDPLAMIFTSGTTGEPKAVLLanrTFFAVPDILQKEGLNwVTWVVGETTYSPLPATHIGGLWW 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 235 IAYVFHRGATnCYL--EDTKHVRDALTTLKPTVMCAVPRFYEKIYTAVwdkvekaplhrralfnwairvgeqhyqieqps 312
Cdd:PRK05857 232 ILTCLMHGGL-CVTggENTTSLLEILTTNAVATTCLVPTLLSKLVSEL-------------------------------- 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 313 qwlrlqyaladKLVLTKLRALlggriKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCW-QDKG----FNPN 387
Cdd:PRK05857 279 -----------KSANATVPSL-----RLVGYGGSRAIAADVRFIEATGVRTAQVYGLSETGCTALCLpTDDGsivkIEAG 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 388 SIGTLMPNAEVKIGEEN----------------EILVRGGMVMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNLFIT 451
Cdd:PRK05857 343 AVGRPYPGVDVYLAATDgigptapgagpsasfgTLWIKSPANMLGYWNNPERTAEVLI-DGWVNTGDLLERREDGFFYIK 421
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 544685990 452 DRLKElMKTSNGKYIAPQYIegkigkDKFIEQIAVIADAKKY 493
Cdd:PRK05857 422 GRSSE-MIICGGVNIAPDEV------DRIAEGVSGVREAACY 456
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
21-498 |
4.76e-12 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 68.45 E-value: 4.76e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:cd12114 2 DATAVICGDG----TLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAARRE 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKiLFVGDQEQFDQVyqIANNCPQLMKIVAMKAnmhlrdlpnacyWEDFLNVVPneaefekrlnskQLSDLFT 180
Cdd:cd12114 78 AILADAGAR-LVLTDGPDAQLD--VAVFDVLILDLDALAA------------PAPPPPVDV------------APDDLAY 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYA---NLAHQLNAHdlaLNVNEDDVSLSFLPLSH------IFE--RAWIAYVFHRGATncyLE 249
Cdd:cd12114 131 VIFTSGSTGTPKGVMISHRaalNTILDINRR---FAVGPDDRVLALSSLSFdlsvydIFGalSAGATLVLPDEAR---RR 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 DTKHVRDALTTLKPTVMCAVPrfyekiytavwdkvekaplhrrALFNWAIRVGEQHyQIEQPSqwLRLQYALADKLVLT- 328
Cdd:cd12114 205 DPAHWAELIERHGVTLWNSVP----------------------ALLEMLLDVLEAA-QALLPS--LRLVLLSGDWIPLDl 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 --KLRALL-GGRIKMMpcGGAKlEASIGSFFHSIG------INIKLGYGMTETTATVScwqdkgfnpNSIGTLMPnaevk 399
Cdd:cd12114 260 paRLRALApDARLISL--GGAT-EASIWSIYHPIDevppdwRSIPYGRPLANQRYRVL---------DPRGRDCP----- 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 400 IGEENEILVRGGMVMRGYYKKPEETAKAFTEDG----FLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGKI 475
Cdd:cd12114 323 DWVPGELWIGGRGVALGYLGDPELTAARFVTHPdgerLYRTGDLGRYRPDGTLEFLGRRDGQVKV-RGYRIELGEIEAAL 401
|
490 500
....*....|....*....|...
gi 544685990 476 GKDKFIEQIAVIADAKKYVSALI 498
Cdd:cd12114 402 QAHPGVARAVVVVLGDPGGKRLA 424
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
181-500 |
9.46e-12 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 66.56 E-value: 9.46e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANL-AHQLNAHDLAlNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYledtkhvrdalt 259
Cdd:cd17636 5 AIYTAAFSGRPNGALLSHQALlAQALVLAVLQ-AIDEGTVFLNSGPLFHIGTLMFTLATFHAGGTNVF------------ 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 260 tlkptvmcaVPRFyekiytavwDKVEKAPLHRRALFNWAIRVGEQHYQIeqpsqwlrLQYALADKLVLTKLRALLGgrik 339
Cdd:cd17636 72 ---------VRRV---------DAEEVLELIEAERCTHAFLLPPTIDQI--------VELNADGLYDLSSLRSSPA---- 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 340 mMPCGGAKLEASIGSFFHSIGiniklGYGMTETTA-TVSCWQDKGFNPNSiGTLMPNAEVKI----------GEENEILV 408
Cdd:cd17636 122 -APEWNDMATVDTSPWGRKPG-----GYGQTEVMGlATFAALGGGAIGGA-GRPSPLVQVRIldedgrevpdGEVGEIVA 194
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 409 RGGMVMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNL-FITDRLKelMKTSNGKYIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:cd17636 195 RGPTVMAGYWNRPEVNARRTR-GGWHHTNDLGRREPDGSLsFVGPKTR--MIKSGAENIYPAEVERCLRQHPAVADAAVI 271
|
330
....*....|....*..
gi 544685990 488 --ADAK--KYVSALIVP 500
Cdd:cd17636 272 gvPDPRwaQSVKAIVVL 288
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
170-575 |
1.05e-11 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 67.46 E-value: 1.05e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 170 LNSKQLSDLFT-------LIYTSGTTGEPKGVMLDYANLA-HQLNAHDLALNVNEDDVsLSFLPLSHIFERAWIAYVFHR 241
Cdd:cd17644 93 LEDAQISVLLTqpenlayVIYTSGSTGKPKGVMIEHQSLVnLSHGLIKEYGITSSDRV-LQFASIAFDVAAEEIYVTLLS 171
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 242 GATncyledtkhvrdalTTLKPTVMCAVPrfyekiyTAVWDKVEKaplhrralfnWAIRVgeqhYQIeQPSQWLRLQYAL 321
Cdd:cd17644 172 GAT--------------LVLRPEEMRSSL-------EDFVQYIQQ----------WQLTV----LSL-PPAYWHLLVLEL 215
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 322 ADKL--VLTKLRALLGGrikmmpcGGAKLEASIGSFFHSIGINIKL--GYGMTETTATVSCW---QDKGFNPNS--IGTL 392
Cdd:cd17644 216 LLSTidLPSSLRLVIVG-------GEAVQPELVRQWQKNVGNFIQLinVYGPTEATIAATVCrltQLTERNITSvpIGRP 288
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 393 MPNAEVKIGEEN----------EILVRGGMVMRGYYKKPEETAKAFTEDGFL--------RTGDVGEMDSCGNLFITDRL 454
Cdd:cd17644 289 IANTQVYILDENlqpvpvgvpgELHIGGVGLARGYLNRPELTAEKFISHPFNsseserlyKTGDLARYLPDGNIEYLGRI 368
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 455 KELMKTsNGKYIAPQYIEGKIGKDKFIEQIAVIA----DAKKYVSALIVPcfdsleeyakqlnikyqdrielikHSDIIQ 530
Cdd:cd17644 369 DNQVKI-RGFRIELGEIEAVLSQHNDVKTAVVIVredqPGNKRLVAYIVP------------------------HYEESP 423
|
410 420 430 440
....*....|....*....|....*....|....*....|....*..
gi 544685990 531 MFERRIHELQKELPSFeqvkrftLLPQAFSTaMEE--ITPTLKLRRK 575
Cdd:cd17644 424 STVELRQFLKAKLPDY-------MIPSAFVV-LEElpLTPNGKIDRR 462
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
17-490 |
1.42e-11 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 66.82 E-value: 1.42e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 17 KKWLNR----TALRFREQaQWqemSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYA 92
Cdd:PRK09029 10 RHWAQVrpqaIALRLNDE-VL---TWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNP 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 93 TNTAKQVEYIVNDADIKILFVGDQEqfdqvyqianncPQLMKIVAmkANMHLRDLPNACYWedflnvvpneaefekrlns 172
Cdd:PRK09029 86 QLPQPLLEELLPSLTLDFALVLEGE------------NTFSALTS--LHLQLVEGAHAVAW------------------- 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 173 kQLSDLFTLIYTSGTTGEPKGVmldyanlAHQLNAHdLA-----LNV----NEDDVSLSfLPLSH-----IFERaWIAyv 238
Cdd:PRK09029 133 -QPQRLATMTLTSGSTGLPKAA-------VHTAQAH-LAsaegvLSLmpftAQDSWLLS-LPLFHvsgqgIVWR-WLY-- 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 239 fhRGATncyLedtkHVRDAlttlkptvmcavprfyEKIYTAVwDKVEKAPLhrralfnwairVgeqhyqieqPSQWLRLq 318
Cdd:PRK09029 200 --AGAT---L----VVRDK----------------QPLEQAL-AGCTHASL-----------V---------PTQLWRL- 232
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 319 yaLADKLVLTKLRALLggrikmmpCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFnpNSIGTLMPNAEV 398
Cdd:PRK09029 233 --LDNRSEPLSLKAVL--------LGGAAIPVELTEQAEQQGIRCWCGYGLTEMASTVCAKRADGL--AGVGSPLPGREV 300
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 399 KIgEENEILVRGGMVMRGYYKKPEETakAFT-EDGFLRTGDVGEMDScGNLFITDRLKElMKTSNGKYIAPQYIEGKIGK 477
Cdd:PRK09029 301 KL-VDGEIWLRGASLALGYWRQGQLV--PLVnDEGWFATRDRGEWQN-GELTILGRLDN-LFFSGGEGIQPEEIERVINQ 375
|
490
....*....|....*
gi 544685990 478 DKFIEQIAV--IADA 490
Cdd:PRK09029 376 HPLVQQVFVvpVADA 390
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
184-487 |
1.68e-11 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 65.84 E-value: 1.68e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 184 TSGTTGEPKGVMLDYANLAHQLNA-HD---------LALnvneddvslsflPLSHIferAWIAYVfhrgatncyledtkh 253
Cdd:PRK07824 43 TSGTTGTPKGAMLTAAALTASADAtHDrlggpgqwlLAL------------PAHHI---AGLQVL--------------- 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 254 VRDALTTLKPTVMcAVPRFYEkiytavwdkVEKAPLHRRALfnwairVGEQHYQIEQPSQWLRLQYALADKLVLTKLRAL 333
Cdd:PRK07824 93 VRSVIAGSEPVEL-DVSAGFD---------PTALPRAVAEL------GGGRRYTSLVPMQLAKALDDPAATAALAELDAV 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 334 LggrikmmpCGGAKLEASIGSFFHSIGINIKLGYGMTETTAtvSCWQDkgfnpnsiGTLMPNAEVKIgEENEILVRGGMV 413
Cdd:PRK07824 157 L--------VGGGPAPAPVLDAAAAAGINVVRTYGMSETSG--GCVYD--------GVPLDGVRVRV-EDGRIALGGPTL 217
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 544685990 414 MRGYYKKPEEtaKAFTEDGFLRTGDVGEMDScGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:PRK07824 218 AKGYRNPVDP--DPFAEPGWFRTDDLGALDD-GVLTVLGRADDAISTG-GLTVLPQVVEAALATHPAVADCAVF 287
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
12-446 |
1.71e-11 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 66.53 E-value: 1.71e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 12 FRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:cd17646 4 VAEQAARTPDAPAVVDEGR----TLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 92 ATNTAKQVEYIVNDADIKILFVGDQEQfdqvyqianncpqlmkivamkanMHLRDLPNACYWEDFLNVVPNEAEFEKRLN 171
Cdd:cd17646 80 PGYPADRLAYMLADAGPAVVLTTADLA-----------------------ARLPAGGDVALLGDEALAAPPATPPLVPPR 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 172 SKQLSdlfTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS---HIFERAWiayVFHRGATncyl 248
Cdd:cd17646 137 PDNLA---YVIYTSGSTGRPKGVMVTHAGIVNRLLWMQDEYPLGPGDRVLQKTPLSfdvSVWELFW---PLVAGAR---- 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 249 edtkhvrdalttlkptVMCAVPRfyekiytavwdkVEKAPLHRRALFNwAIRVGEQHYQieqPSQwLRLQYALADKLVLT 328
Cdd:cd17646 207 ----------------LVVARPG------------GHRDPAYLAALIR-EHGVTTCHFV---PSM-LRVFLAEPAAGSCA 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 KLRALLggrikmmpCGGAKLEASIGSFFHSI-GINIKLGYGMTETTATVSCWQ---DKGFNPNSIGTLMPNAEVKI---- 400
Cdd:cd17646 254 SLRRVF--------CSGEALPPELAARFLALpGAELHNLYGPTEAAIDVTHWPvrgPAETPSVPIGRPVPNTRLYVldda 325
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 544685990 401 ------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGF------LRTGDV------GEMDSCG 446
Cdd:cd17646 326 lrpvpvGVPGELYLGGVQLARGYLGRPALTAERFVPDPFgpgsrmYRTGDLarwrpdGALEFLG 389
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
174-448 |
1.73e-11 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 66.80 E-value: 1.73e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 174 QLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS---HIFErawIAYVFHRGATNCYL-E 249
Cdd:cd05918 104 SPSDAAYVIFTSGSTGKPKGVVIEHRALSTSALAHGRALGLTSESRVLQFASYTfdvSILE---IFTTLAAGGCLCIPsE 180
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 DTKHVR--DALTTLKPTVMCAVPRFyekiytAvwdkvekaplhrRALfnwairvgeqhyqieQPSQWLRLQYaladkLVL 327
Cdd:cd05918 181 EDRLNDlaGFINRLRVTWAFLTPSV------A------------RLL---------------DPEDVPSLRT-----LVL 222
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 328 tklrallggrikmmpcGGAKLEASIGSFFHSigiNIKL--GYGMTETT-ATVSCWQDKGFNPNSIGTLM----------- 393
Cdd:cd05918 223 ----------------GGEALTQSDVDTWAD---RVRLinAYGPAECTiAATVSPVVPSTDPRNIGRPLgatcwvvdpdn 283
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 394 PNAEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDG-------------FLRTGDVGEMDSCGNL 448
Cdd:cd05918 284 HDRLVPIGAVGELLIEGPILARGYLNDPEKTAAAFIEDPawlkqegsgrgrrLYRTGDLVRYNPDGSL 351
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
61-472 |
2.14e-11 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 66.64 E-value: 2.14e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 61 DKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILfVGDQEQFDQVYQIANNCPQL-MKIVAMK 139
Cdd:PRK13391 50 DHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAEAAYIVDDSGARAL-ITSAAKLDVARALLKQCPGVrHRLVLDG 128
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 140 ANmhlrDLPNacyWEDFLNVVpnEAEFEKRLNSKQLSDLftLIYTSGTTGEPKGVM--LDYANLAHQLNAHDLALN---V 214
Cdd:PRK13391 129 DG----ELEG---FVGYAEAV--AGLPATPIADESLGTD--MLYSSGTTGRPKGIKrpLPEQPPDTPLPLTAFLQRlwgF 197
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 215 NEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLEdtkhvrdalttlkptvmcavpRFYEKIYTAVwdkVEKaplhrral 294
Cdd:PRK13391 198 RSDMVYLSPAPLYHSAPQRAVMLVIRLGGTVIVME---------------------HFDAEQYLAL---IEE-------- 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 295 fnWAIrvgeQHYQIeQP---SQWLRLQYALADKLVLTKLRALLGGrikMMPCGGAKLEASI---GSFFHSIginiklgYG 368
Cdd:PRK13391 246 --YGV----THTQL-VPtmfSRMLKLPEEVRDKYDLSSLEVAIHA---AAPCPPQVKEQMIdwwGPIIHEY-------YA 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 369 MTETTATVSC----WQDkgfNPNSIGTLM---------PNAEVKIGEENEILVRGGMVMRgYYKKPEETAKAFTEDGFLR 435
Cdd:PRK13391 309 ATEGLGFTACdseeWLA---HPGTVGRAMfgdlhilddDGAELPPGEPGTIWFEGGRPFE-YLNDPAKTAEARHPDGTWS 384
|
410 420 430
....*....|....*....|....*....|....*...
gi 544685990 436 T-GDVGEMDSCGNLFITDRlKELMKTSNGKYIAPQYIE 472
Cdd:PRK13391 385 TvGDIGYVDEDGYLYLTDR-AAFMIISGGVNIYPQEAE 421
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
23-500 |
3.84e-11 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 66.52 E-value: 3.84e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 23 TALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYI 102
Cdd:PRK12316 528 PALAFGEE----TLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPAERLAYM 603
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 103 VNDADIKILFvgdQEQFDQvyqianncpQLMKIVAMKANMHLrDLPNAcywedFLNVVPNEAEfEKRLNSKQLSdlfTLI 182
Cdd:PRK12316 604 LEDSGVQLLL---SQSHLG---------RKLPLAAGVQVLDL-DRPAA-----WLEGYSEENP-GTELNPENLA---YVI 661
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 183 YTSGTTGEPKGVMLDYANLAHQLNahdlalnvneddvslsflplshiferaWIAYVFHRGATNCYLEDTKHVRDAlttlk 262
Cdd:PRK12316 662 YTSGSTGKPKGAGNRHRALSNRLC---------------------------WMQQAYGLGVGDTVLQKTPFSFDV----- 709
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 263 ptvmcAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAI----RVGEQHYQieqPSQWLRLQYALADKLVLTKLRALLGGRi 338
Cdd:PRK12316 710 -----SVWEFFWPLMSGARLVVAAPGDHRDPAKLVELinreGVDTLHFV---PSMLQAFLQDEDVASCTSLRRIVCSGE- 780
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 339 kmmPCGGAKLEASIGSFFHSIGINIklgYGMTETTATVSCWQ--DKGFNPNSIGTLMPNAE----------VKIGEENEI 406
Cdd:PRK12316 781 ---ALPADAQEQVFAKLPQAGLYNL---YGPTEAAIDVTHWTcvEEGGDSVPIGRPIANLAcyildanlepVPVGVLGEL 854
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 407 LVRGGMVMRGYYKKPEETAKAFTEDGFL------RTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGKIGKDKF 480
Cdd:PRK12316 855 YLAGRGLARGYHGRPGLTAERFVPSPFVagermyRTGDLARYRADGVIEYAGRIDHQVKL-RGLRIELGEIEARLLEHPW 933
|
490 500
....*....|....*....|
gi 544685990 481 IEQIAVIADAKKYVSALIVP 500
Cdd:PRK12316 934 VREAAVLAVDGKQLVGYVVL 953
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
177-487 |
4.02e-11 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 64.35 E-value: 4.02e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLE--DTKHV 254
Cdd:cd17633 1 NPFYIGFTSGTTGLPKAYYRSERSWIESFVCNEDLFNISGEDAILAPGPLSHSLFLYGAISALYLGGTFIGQRkfNPKSW 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 255 RDALTTLKPTVMCAVPRFYEKIYtavwdkvekaplhrralfnwairvgeQHYQIEQPsqwlrlqyaladklvltklrall 334
Cdd:cd17633 81 IRKINQYNATVIYLVPTMLQALA--------------------------RTLEPESK----------------------- 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 335 ggrIKMMPCGGAKLEASIGSFFHSI--GINIKLGYGMTETT-ATVSCWQDKGfNPNSIGTLMPNAEVKI-----GEENEI 406
Cdd:cd17633 112 ---IKSIFSSGQKLFESTKKKLKNIfpKANLIEFYGTSELSfITYNFNQESR-PPNSVGRPFPNVEIEIrnadgGEIGKI 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 407 LVRGGMVMRGYYKkpeetAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDKFIEQIAV 486
Cdd:cd17633 188 FVKSEMVFSGYVR-----GGFSNPDGWMSVGDIGYVDEEGYLYLVGRESD-MIIIGGINIFPTEIESVLKAIPGIEEAIV 261
|
.
gi 544685990 487 I 487
Cdd:cd17633 262 V 262
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
11-438 |
4.91e-11 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 66.34 E-value: 4.91e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 11 RFRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI 90
Cdd:PRK12467 1579 LIEDQAAATPEAVALVFGEQ----ELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPL 1654
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 91 YATNTAKQVEYIVNDADIKILFVgdQEQFDQVYQIANNCPQLmkivamkanmhLRDLPNAcyWEDflnvVPNEAEFEKRL 170
Cdd:PRK12467 1655 DPEYPRERLAYMIEDSGIELLLT--QSHLQARLPLPDGLRSL-----------VLDQEDD--WLE----GYSDSNPAVNL 1715
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 171 NSKQLSdlfTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS---HIFERAW----------IAY 237
Cdd:PRK12467 1716 APQNLA---YVIYTSGSTGRPKGAGNRHGALVNRLCATQEAYQLSAADVVLQFTSFAfdvSVWELFWplingarlviAPP 1792
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 238 VFHRGAtncylEDTKHVrdaLTTLKPTVMCAVPRFYEKIytavwdkVEKAPlhrralfnwairvgeqhyQIEQPsqwlrl 317
Cdd:PRK12467 1793 GAHRDP-----EQLIQL---IERQQVTTLHFVPSMLQQL-------LQMDE------------------QVEHP------ 1833
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 318 qyaladklvlTKLRALLggrikmmpCGGAKLEA-SIGSFFHSIGIN-IKLGYGMTETTATVSCW-----QDKGFNPNSIG 390
Cdd:PRK12467 1834 ----------LSLRRVV--------CGGEALEVeALRPWLERLPDTgLFNLYGPTETAVDVTHWtcrrkDLEGRDSVPIG 1895
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 391 TLMPN----------AEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGD 438
Cdd:PRK12467 1896 QPIANlstyildaslNPVPIGVAGELYLGGVGLARGYLNRPALTAERFVADPFgtvgsrlYRTGD 1960
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
18-193 |
5.98e-11 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 65.20 E-value: 5.98e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 18 KWL----NRTALRFR-EQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYA 92
Cdd:cd05968 69 KWLadtrTRPALRWEgEDGTSRTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFS 148
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 93 TNTAKQVEYIVNDADIKILFVGDQ--------EQFDQVYQIANNCPQLMKIVAMKanmHL-RDLPNACY----WEDFLNV 159
Cdd:cd05968 149 GFGKEAAATRLQDAEAKALITADGftrrgrevNLKEEADKACAQCPTVEKVVVVR---HLgNDFTPAKGrdlsYDEEKET 225
|
170 180 190
....*....|....*....|....*....|....
gi 544685990 160 VPNEAEfekRLNSKqlsDLFTLIYTSGTTGEPKG 193
Cdd:cd05968 226 AGDGAE---RTESE---DPLMIIYTSGTTGKPKG 253
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
57-487 |
6.04e-11 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 65.19 E-value: 6.04e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 57 IDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILfVGDQEQFDQVYQIANNCPQLMKIV 136
Cdd:PRK05620 61 ITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIVHIINHAEDEVI-VADPRLAEQLGEILKECPCVRAVV 139
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 137 ------AMKANMHLRDLPNACYWEDFLN---VVPNEAEFEKRlnskqlsDLFTLIYTSGTTGEPKGVMLDYANL---AHQ 204
Cdd:PRK05620 140 figpsdADSAAAHMPEGIKVYSYEALLDgrsTVYDWPELDET-------TAAAICYSTGTTGAPKGVVYSHRSLylqSLS 212
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 205 LNAHDlALNVNEDDVSLSFLPLSHIFE-----RAWIA---YVFhRGATncylEDTKHVRDALTTLKPTVMCAVPrfyeki 276
Cdd:PRK05620 213 LRTTD-SLAVTHGESFLCCVPIYHVLSwgvplAAFMSgtpLVF-PGPD----LSAPTLAKIIATAMPRVAHGVP------ 280
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 277 ytAVWDKvekaplhrraLFnwairvgeQHYQIEQPSQwlrlqyaladklvlTKLRALLGGrikmmpcGGAKLEASIGSFF 356
Cdd:PRK05620 281 --TLWIQ----------LM--------VHYLKNPPER--------------MSLQEIYVG-------GSAVPPILIKAWE 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 357 HSIGINIKLGYGMTETTA--TVS------CWQDKGFNPNSIGTLMPNAEVKI---GE--------ENEILVRGGMVMRGY 417
Cdd:PRK05620 320 ERYGVDVVHVWGMTETSPvgTVArppsgvSGEARWAYRVSQGRFPASLEYRIvndGQvmestdrnEGEIQVRGNWVTASY 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 418 YKKP----------------EETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNGKYIAPQYIEGKIGKDKFI 481
Cdd:PRK05620 400 YHSPteegggaastfrgedvEDANDRFTADGWLRTGDVGSVTRDGFLTIHDRARDVIR-SGGEWIYSAQLENYIMAAPEV 478
|
....*.
gi 544685990 482 EQIAVI 487
Cdd:PRK05620 479 VECAVI 484
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
182-500 |
7.18e-11 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 64.84 E-value: 7.18e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 182 IYTSGTTGEPKGVMLDYANLAHQ--LNAHDLALNVNEDDVSLSFLPLSH-IFERAWIAYVFHRGATNCYLEDTKHVrDAL 258
Cdd:cd05923 156 FYTSGTTGLPKGAVIPQRAAESRvlFMSTQAGLRHGRHNVVLGLMPLYHvIGFFAVLVAALALDGTYVVVEEFDPA-DAL 234
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 259 TTLKP---TVMCAVPRFYEKIYTAVwdkvEKAPLHRRALfnwairvgeqhyqieqpsQWLRLQYALADKLVLTKLRALLG 335
Cdd:cd05923 235 KLIEQervTSLFATPTHLDALAAAA----EFAGLKLSSL------------------RHVTFAGATMPDAVLERVNQHLP 292
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 336 GRIkmmpcggakleasigsffhsigINIklgYGMTETTATVscwqdkgFNPN-SIGTLMP---NAEVKI----------- 400
Cdd:cd05923 293 GEK----------------------VNI---YGTTEAMNSL-------YMRDaRTGTEMRpgfFSEVRIvriggspdeal 340
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 --GEENEILVR--GGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIG 476
Cdd:cd05923 341 anGEEGELIVAaaADAAFTGYLNQPEATAKKL-QDGWYRTGDVGYVDPSGDVRILGRVDD-MIISGGENIHPSEIERVLS 418
|
330 340
....*....|....*....|....*...
gi 544685990 477 KDKFIEQIAVI--ADAK--KYVSALIVP 500
Cdd:cd05923 419 RHPGVTEVVVIgvADERwgQSVTACVVP 446
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
34-500 |
1.01e-10 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 64.03 E-value: 1.01e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 34 QEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFv 113
Cdd:cd17656 12 QKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIMLDSGVRVVL- 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 114 gdqeqfdqvyqianNCPQLMKIVAMKANMHLRDLPNAcYWEDFLNV-VPNEAEfekrlnskqlsDLFTLIYTSGTTGEPK 192
Cdd:cd17656 91 --------------TQRHLKSKLSFNKSTILLEDPSI-SQEDTSNIdYINNSD-----------DLLYIIYTSGTTGKPK 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 193 GVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS------HIFErAWIAyvfhrGATnCYL--EDTKHVRDALTTL--- 261
Cdd:cd17656 145 GVQLEHKNMVNLLHFEREKTNINFSDKVLQFATCSfdvcyqEIFS-TLLS-----GGT-LYIirEETKRDVEQLFDLvkr 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 262 KPTVMCAVPrfyekiyTAVWdkveKAPLHRRALFN-------WAIRVGEQhYQIEQPSQwlrlqyaladklvltklrall 334
Cdd:cd17656 218 HNIEVVFLP-------VAFL----KFIFSEREFINrfptcvkHIITAGEQ-LVITNEFK--------------------- 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 335 ggrikmmpcggakleasigSFFHSIGINIKLGYGMTETTATVSCwqdkGFNPNSIGTLMP-------NAEVKIGEEN--- 404
Cdd:cd17656 265 -------------------EMLHEHNVHLHNHYGPSETHVVTTY----TINPEAEIPELPpigkpisNTWIYILDQEqql 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 -------EILVRGGMVMRGYYKKPEETAKAFTEDGF------LRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYI 471
Cdd:cd17656 322 qpqgivgELYISGASVARGYLNRQELTAEKFFPDPFdpnermYRTGDLARYLPDGNIEFLGRADHQVKI-RGYRIELGEI 400
|
490 500 510
....*....|....*....|....*....|...
gi 544685990 472 EGKIGKDKFIEQIAVIADA----KKYVSALIVP 500
Cdd:cd17656 401 EAQLLNHPGVSEAVVLDKAddkgEKYLCAYFVM 433
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
367-579 |
1.49e-10 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 63.76 E-value: 1.49e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 367 YGMTETTATVSCWQ------DKgFNPNSIGTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAF-T 429
Cdd:PRK04813 293 YGPTEATVAVTSIEitdemlDQ-YKRLPIGYAKPDSPLLIideegtklpdGEQGEIVISGPSVSKGYLNNPEKTAEAFfT 371
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 430 EDG--FLRTGDVGEMDScGNLFITDRLKELMKTsNGKYIAPQYIEGKIGKDKFIEQiAVIADAKK-----YVSALIVPCF 502
Cdd:PRK04813 372 FDGqpAYHTGDAGYLED-GLLFYQGRIDFQIKL-NGYRIELEEIEQNLRQSSYVES-AVVVPYNKdhkvqYLIAYVVPKE 448
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 503 DSLE-EYAKQLNIKyqdrielikhsdiiqmferriHELQKELPSFeqvkrftLLPQAFsTAMEEI--TPTLKLRRKVIMQ 579
Cdd:PRK04813 449 EDFErEFELTKAIK---------------------KELKERLMEY-------MIPRKF-IYRDSLplTPNGKIDRKALIE 499
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
37-490 |
1.75e-10 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 63.71 E-value: 1.75e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 37 SWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgDQ 116
Cdd:PLN02479 47 TWAQTYQRCRRLASALAKRSIGPGSTVAVIAPNIPAMYEAHFGVPMAGAVVNCVNIRLNAPTIAFLLEHSKSEVVMV-DQ 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 117 EQF---DQVYQI-----ANNCPQLMKIVAMKANMHLRDLPNA-----CYWEDFLNVvpNEAEFekrlNSKQLSDLFTLI- 182
Cdd:PLN02479 126 EFFtlaEEALKIlaekkKSSFKPPLLIVIGDPTCDPKSLQYAlgkgaIEYEKFLET--GDPEF----AWKPPADEWQSIa 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 183 --YTSGTTGEPKGVMLdyanlaHQLNAHDLALN------VNEDDVSLSFLPLSHIfeRAWI---AYVFHRGATNCYLE-D 250
Cdd:PLN02479 200 lgYTSGTTASPKGVVL------HHRGAYLMALSnaliwgMNEGAVYLWTLPMFHC--NGWCftwTLAALCGTNICLRQvT 271
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 251 TKHVRDALTTLKPTVMCAVPrfyekiytAVWDKVEKAPLHRRALfnwairvgeqhyqieqPsqwlrlqyaladklvltkl 330
Cdd:PLN02479 272 AKAIYSAIANYGVTHFCAAP--------VVLNTIVNAPKSETIL----------------P------------------- 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 331 ralLGGRIKMMPCGGAKlEASIGSFFHSIGINIKLGYGMTET--TATVSCWQDK--GFNPNSIGTLMPNAEVK-IGEEN- 404
Cdd:PLN02479 309 ---LPRVVHVMTAGAAP-PPSVLFAMSEKGFRVTHTYGLSETygPSTVCAWKPEwdSLPPEEQARLNARQGVRyIGLEGl 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 -------------------EILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKY 465
Cdd:PLN02479 385 dvvdtktmkpvpadgktmgEIVMRGNMVMKGYLKNPKANEEAF-ANGWFHSGDLGVKHPDGYIEIKDRSKDII-ISGGEN 462
|
490 500
....*....|....*....|....*
gi 544685990 466 IAPQYIEGKIGKDKFIEQIAVIADA 490
Cdd:PLN02479 463 ISSLEVENVVYTHPAVLEASVVARP 487
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
46-512 |
1.78e-10 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 63.22 E-value: 1.78e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 46 DRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILfvgdqeqfdqvyqi 125
Cdd:cd05971 17 NRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASAL-------------- 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 126 anncpqlmkivamkanmhLRDLPNacywedflnvvpneaefekrlnskqlsDLFTLIYTSGTTGEPKGVMLDYANLAHQL 205
Cdd:cd05971 83 ------------------VTDGSD---------------------------DPALIIYTSGTTGPPKGALHAHRVLLGHL 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 206 NAHDLALNVNEDDVSLSFLPLshifERAWIAYVF-------HRGAtncyledtkhvrdalttlkPTVMCAVPRFY-EKIY 277
Cdd:cd05971 118 PGVQFPFNLFPRDGDLYWTPA----DWAWIGGLLdvllpslYFGV-------------------PVLAHRMTKFDpKAAL 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 278 TAVWD-KVEKAPLHRRALfnwaiRVGEQHYqiEQPSQWLRlqyaladklvltKLRALLggrikmmpCGGAKL-EASIGSF 355
Cdd:cd05971 175 DLMSRyGVTTAFLPPTAL-----KMMRQQG--EQLKHAQV------------KLRAIA--------TGGESLgEELLGWA 227
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 356 FHSIGINIKLGYGMTETTATV-SCWQDKGFNPNSIGTLMPNAEVKI----------GEENEILVR--GGMVMRGYYKKPE 422
Cdd:cd05971 228 REQFGVEVNEFYGQTECNLVIgNCSALFPIKPGSMGKPIPGHRVAIvddngtplppGEVGEIAVElpDPVAFLGYWNNPS 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 423 ETAKAFTEDgFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVIA--DAKK--YVSALI 498
Cdd:cd05971 308 ATEKKMAGD-WLLTGDLGRKDSDGYFWYVGRDDDVITSS-GYRIGPAEIEECLLKHPAVLMAAVVGipDPIRgeIVKAFV 385
|
490
....*....|....*.
gi 544685990 499 V--PCFDSLEEYAKQL 512
Cdd:cd05971 386 VlnPGETPSDALAREI 401
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
7-500 |
2.10e-10 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 64.21 E-value: 2.10e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 7 HFVN-RFRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARA 85
Cdd:PRK12316 2003 PGVHqRIAEQAARAPEAIAVVFGDQ----HLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGG 2078
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 86 VAVPIYATNTAKQVEYIVNDADIKILFVgdQEQFDQVYQIANNCPQLmkivamkanmhlrDLPNACYWEDFLNVVPneae 165
Cdd:PRK12316 2079 AYVPLDPNYPAERLAYMLEDSGAALLLT--QRHLLERLPLPAGVARL-------------PLDRDAEWADYPDTAP---- 2139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 166 fekrLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS-HIFERAWI------AYV 238
Cdd:PRK12316 2140 ----AVQLAGENLAYVIYTSGSTGLPKGVAVSHGALVAHCQAAGERYELSPADCELQFMSFSfDGAHEQWFhpllngARV 2215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 239 FHRGATncyLEDTKHVRDALTTLKPTVMCAVPRFYEKIytavwdkVEKAPLHRRALfnwAIRV----GEQhyqieQPSQW 314
Cdd:PRK12316 2216 LIRDDE---LWDPEQLYDEMERHGVTILDFPPVYLQQL-------AEHAERDGRPP---AVRVycfgGEA-----VPAAS 2277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 315 LRLQyaladklvltkLRALLGGRIkmmpcggakleasigsffhsigINiklGYGMTETTATVS----CWQDK-GFNPNSI 389
Cdd:PRK12316 2278 LRLA-----------WEALRPVYL----------------------FN---GYGPTEAVVTPLlwkcRPQDPcGAAYVPI 2321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 390 GTLMPNAEVKIGEENEILVRGGM----------VMRGYYKKPEETAKAFTEDGF-------LRTGDVGEMDSCGNLFITD 452
Cdd:PRK12316 2322 GRALGNRRAYILDADLNLLAPGMagelylggegLARGYLNRPGLTAERFVPDPFsasgerlYRTGDLARYRADGVVEYLG 2401
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 544685990 453 RLKELMKTsNGKYIAPQYIEGKIGKDKFIEQIAVIADAK---KYVSALIVP 500
Cdd:PRK12316 2402 RIDHQVKI-RGFRIELGEIEARLQAHPAVREAVVVAQDGasgKQLVAYVVP 2451
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
396-472 |
2.31e-10 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 63.49 E-value: 2.31e-10
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990 396 AEVKIGEeneILVRGGMVMRGYYKKpEETAKAFTEDGFLRTGDVGEMdSCGNLFITDRLKELMkTSNGKYIAPQYIE 472
Cdd:PRK09192 407 PERVVGH---ICVRGPSLMSGYFRD-EESQDVLAADGWLDTGDLGYL-LDGYLYITGRAKDLI-IINGRNIWPQDIE 477
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
19-544 |
2.36e-10 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 63.25 E-value: 2.36e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 19 WLNRTAlrfreqaqwQEMSWqTFQQ--EIDRFSYALIAQHIDIQ--DKIGIFANNMPRWTIADFGAMQARAVAVPIYATN 94
Cdd:cd05928 32 WVNGKG---------DEVKW-SFRElgSLSRKAANVLSGACGLQrgDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQL 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 95 TAKQVEYIVNDADIKILFVGDqEQFDQVYQIANNCPQLMkiVAMKANMHLRDlpnacYWEDFLNVVpNEAEFEKRLNSKQ 174
Cdd:cd05928 102 TAKDILYRLQASKAKCIVTSD-ELAPEVDSVASECPSLK--TKLLVSEKSRD-----GWLNFKELL-NEASTEHHCVETG 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 175 LSDLFTLIYTSGTTGEPKGVMLDYANLAH----------QLNAHDLALNVNedDVSLSFLPLSHIFErAWI--AYVF-HR 241
Cdd:cd05928 173 SQEPMAIYFTSGTTGSPKMAEHSHSSLGLglkvngrywlDLTASDIMWNTS--DTGWIKSAWSSLFE-PWIqgACVFvHH 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 242 GATNcyleDTKHVRDALTTLKPTVMCAVPRFYEKIytaVWDKVEKaplhrralfnwairvgeqhYQIEqpsqwlRLQYAL 321
Cdd:cd05928 250 LPRF----DPLVILKTLSSYPITTFCGAPTVYRML---VQQDLSS-------------------YKFP------SLQHCV 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 322 adklvltklrallggrikmmpCGGAKLEASI-GSFFHSIGINIKLGYGMTETtaTVSCWQDKG--FNPNSIGTLMPNAEV 398
Cdd:cd05928 298 ---------------------TGGEPLNPEVlEKWKAQTGLDIYEGYGQTET--GLICANFKGmkIKPGSMGKASPPYDV 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 399 KI----------GEENEILVRGGMV-----MRGYYKKPEETAKAFTEDgFLRTGDVGEMDSCGNLFITDRLKELMKTSnG 463
Cdd:cd05928 355 QIiddngnvlppGTEGDIGIRVKPIrpfglFSGYVDNPEKTAATIRGD-FYLTGDRGIMDEDGYFWFMGRADDVINSS-G 432
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 464 KYIAPQYIEGKIGKDKFIEQIAVIADAK----KYVSALIVPCFDSLEEYAKQLNIKYQDRielIKHSDIIQMFERRIhEL 539
Cdd:cd05928 433 YRIGPFEVESALIEHPAVVESAVVSSPDpirgEVVKAFVVLAPQFLSHDPEQLTKELQQH---VKSVTAPYKYPRKV-EF 508
|
....*
gi 544685990 540 QKELP 544
Cdd:cd05928 509 VQELP 513
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
61-473 |
4.68e-10 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 62.23 E-value: 4.68e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 61 DKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgDQEQFDQVYQIANNCPQLMKIVAMKA 140
Cdd:PRK08276 37 DVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVLIV-SAALADTAAELAAELPAGVPLLLVVA 115
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 141 NmhlrDLPNACYWEDFLNVVPNEAEFEKRLNSkqlsdlfTLIYTSGTTGEPKGVM-------LDYANLAHqLNAHDLALN 213
Cdd:PRK08276 116 G----PVPGFRSYEEALAAQPDTPIADETAGA-------DMLYSSGTTGRPKGIKrplpgldPDEAPGMM-LALLGFGMY 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 214 VNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLEdtkhvR-DALTTLKptvmcavprfyekiytavwdkvekaplhrr 292
Cdd:PRK08276 184 GGPDSVYLSPAPLYHTAPLRFGMSALALGGTVVVME-----KfDAEEALA------------------------------ 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 293 alfnwAIrvgeQHYQIEQpSQW--------LRLQYALADKLVLTKLR-ALLGGrikmMPCGGAKLEASI---GSFFHSIg 360
Cdd:PRK08276 229 -----LI----ERYRVTH-SQLvptmfvrmLKLPEEVRARYDVSSLRvAIHAA----APCPVEVKRAMIdwwGPIIHEY- 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 361 iniklgYGMTE----TTATVSCWQDKgfnPNSIGTLMpNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAK 426
Cdd:PRK08276 294 ------YASSEgggvTVITSEDWLAH---PGSVGKAV-LGEVRIldedgnelppGEIGTVYFEMDGYPFEYHNDPEKTAA 363
|
410 420 430 440
....*....|....*....|....*....|....*....|....*..
gi 544685990 427 AFTEDGFLRTGDVGEMDSCGNLFITDRlKELMKTSNGKYIAPQYIEG 473
Cdd:PRK08276 364 ARNPHGWVTVGDVGYLDEDGYLYLTDR-KSDMIISGGVNIYPQEIEN 409
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
22-554 |
8.53e-10 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 61.34 E-value: 8.53e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 22 RTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQ-DKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:cd05958 1 RTCLRSPER----EWTYRDLLALANRIANVLVGELGIVPgNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELA 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFVGDQEQfdqvyqianncpqlmkivamkanmhLRDlpnacywedflnvvpneaefekrlnskqlsDLFT 180
Cdd:cd05958 77 YILDKARITVALCAHALT-------------------------ASD------------------------------DICI 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVM---LDYANLAHQLNAHDLALnvNEDDVSLSFLPLSHIFERAWIA-YVFHRGATNCYLEDT--KHV 254
Cdd:cd05958 102 LAFTSGTTGAPKATMhfhRDPLASADRYAVNVLRL--REDDRFVGSPPLAFTFGLGGVLlFPFGVGASGVLLEEAtpDLL 179
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 255 RDALTTLKPTVMCAVPRFYEKIytavwdkVEKAPLHRRalfnwairvgeqhyqieqpsqwlrlqyaladklvltklraLL 334
Cdd:cd05958 180 LSAIARYKPTVLFTAPTAYRAM-------LAHPDAAGP----------------------------------------DL 212
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 335 GGRIKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI----------GEEN 404
Cdd:cd05958 213 SSLRKCVSAGEALPAALHRAWKEATGIPIIDGIGSTEMFHIFISARPGDARPGATGKPVPGYEAKVvddegnpvpdGTIG 292
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 EILVRGGMvmrGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNGKYIAPQYIEGKIGKDKFIEQI 484
Cdd:cd05958 293 RLAVRGPT---GCRYLADKRQRTYVQGGWNITGDTYSRDPDGYFRHQGRSDDMIV-SGGYNIAPPEVEDVLLQHPAVAEC 368
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 485 AVIA----DAKKYVSALIVP---------CFDSLEEYAKQLNIKYQdrielikhsdiiqmFERRIhELQKELPSFE--QV 549
Cdd:cd05958 369 AVVGhpdeSRGVVVKAFVVLrpgvipgpvLARELQDHAKAHIAPYK--------------YPRAI-EFVTELPRTAtgKL 433
|
....*
gi 544685990 550 KRFTL 554
Cdd:cd05958 434 QRFAL 438
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
40-487 |
1.05e-09 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 60.98 E-value: 1.05e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 40 TFQQ---EIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgdq 116
Cdd:cd05969 2 TFAQlkvLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLIT--- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 117 eqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpNEAEFEKRlnskQLSDLFTLIYTSGTTGEPKGVML 196
Cdd:cd05969 79 ---------------------------------------------TEELYERT----DPEDPTLLHYTSGTTGTPKGVLH 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 197 DYANLAHQLNAHDLALNVNEDDVslsflpLSHIFERAWIAYVFH-------RGATNCYLEDTKHVR---DALTTLKPTVM 266
Cdd:cd05969 110 VHDAMIFYYFTGKYVLDLHPDDI------YWCTADPGWVTGTVYgiwapwlNGVTNVVYEGRFDAEswyGIIERVKVTVW 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 267 cavprfyekiYTAvwdkvekaPLHRRALfnwairvgeqhyqieqpsqwLRLQYALADKLVLTKLRALLGGrikmmpcgGA 346
Cdd:cd05969 184 ----------YTA--------PTAIRML--------------------MKEGDELARKYDLSSLRFIHSV--------GE 217
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 347 KLEASIGSFFHSI-GINIKLGYGMTETTATVSC-WQDKGFNPNSIGTLMPNAEVKI----------GEENEILVRGGM-- 412
Cdd:cd05969 218 PLNPEAIRWGMEVfGVPIHDTWWQTETGSIMIAnYPCMPIKPGSMGKPLPGVKAAVvdengnelppGTKGILALKPGWps 297
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 413 VMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:cd05969 298 MFRGIWNDEERYKNSFI-DGWYLTGDLAYRDEDGYFWFVGRADDIIKTS-GHRVGPFEVESALMEHPAVAEAGVI 370
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
360-500 |
1.63e-09 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 60.39 E-value: 1.63e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 360 GINIKLGYGMTETTATVSCWQDKGF--NPNSIGTLMPNAEVKI--GEENEILVRGGMVMRGYYKKPEETAKAFTedgflr 435
Cdd:PRK07445 254 QLRLAPTYGMTETASQIATLKPDDFlaGNNSSGQVLPHAQITIpaNQTGNITIQAQSLALGYYPQILDSQGIFE------ 327
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 544685990 436 TGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKY----VSALIVP 500
Cdd:PRK07445 328 TDDLGYLDAQGYLHILGRNSQKI-ITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHwgevVTAIYVP 395
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
182-465 |
2.90e-09 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 59.91 E-value: 2.90e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 182 IYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAW-IAYV-----FHRGATncylEDTKHVR 255
Cdd:PRK09274 180 LFTSGSTGTPKGVVYTHGMFEAQIEALREDYGIEPGEIDLPTFPLFALFGPALgMTSVipdmdPTRPAT----VDPAKLF 255
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 256 DALTTLKPTVMCAVPrfyekiytAVWDKVekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADKLVLTKLRALLg 335
Cdd:PRK09274 256 AAIERYGVTNLFGSP--------ALLERL--------------------------------GRYGEANGIKLPSLRRVI- 294
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 336 grikmmpCGGAKLEASIGSFFHSI---GINIKLGYGMTET------------TATVSCWqDKGFNpNSIGTLMPNAEVKI 400
Cdd:PRK09274 295 -------SAGAPVPIAVIERFRAMlppDAEILTPYGATEAlpissiesreilFATRAAT-DNGAG-ICVGRPVDGVEVRI 365
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -------------------GEENEILVRGGMVMRGYYKKPEETAKAFTEDG----FLRTGDVGEMDSCGNLFITDRLKEL 457
Cdd:PRK09274 366 iaisdapipewddalrlatGEIGEIVVAGPMVTRSYYNRPEATRLAKIPDGqgdvWHRMGDLGYLDAQGRLWFCGRKAHR 445
|
....*...
gi 544685990 458 MKTSNGKY 465
Cdd:PRK09274 446 VETAGGTL 453
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
36-511 |
3.87e-09 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 59.39 E-value: 3.87e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 36 MSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgD 115
Cdd:PRK13382 69 LTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGADILLLNTSFAGPALAEVVTREGVDTVIY-D 147
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 116 QEQFDQVYQIANNCPQLMKIVAmkanmhlrdlpnacyWEDFLNVVPNEAEFEKRLN-----SKQLSDlfTLIYTSGTTGE 190
Cdd:PRK13382 148 EEFSATVDRALADCPQATRIVA---------------WTDEDHDLTVEVLIAAHAGqrpepTGRKGR--VILLTSGTTGT 210
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 191 PKGVmldyanlahqlnahdlalnvnEDDVSLSFLPLSHIFERA-W--------IAYVFHR---------GATNCYL---- 248
Cdd:PRK13382 211 PKGA---------------------RRSGPGGIGTLKAILDRTpWraeeptviVAPMFHAwgfsqlvlaASLACTIvtrr 269
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 249 ----EDTKHVRDALttlKPTVMCAVPRFYEKIYTAVwDKVeKAPLHRRALfnwairvgeqhyqieqpsqwlrlqyaladk 324
Cdd:PRK13382 270 rfdpEATLDLIDRH---RATGLAVVPVMFDRIMDLP-AEV-RNRYSGRSL------------------------------ 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 325 lvltKLRALLGGRikMMPcggakleASIGSFFHSIGINIKLGYGMTE----TTATVscwQDKGFNPNSIGTLMPNAEVKI 400
Cdd:PRK13382 315 ----RFAAASGSR--MRP-------DVVIAFMDQFGDVIYNNYNATEagmiATATP---ADLRAAPDTAGRPAEGTEIRI 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 ----------GEENEILVRGGMVMRGYYKKpeeTAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQY 470
Cdd:PRK13382 379 ldqdfrevptGEVGTIFVRNDTQFDGYTSG---STKDF-HDGFMASGDVGYLDENGRLFVVGRDDE-MIVSGGENVYPIE 453
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 544685990 471 IEGKIGKDKFIEQIAVIA-DAKKY---VSALIVPCFDS--LEEYAKQ 511
Cdd:PRK13382 454 VEKTLATHPDVAEAAVIGvDDEQYgqrLAAFVVLKPGAsaTPETLKQ 500
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
6-500 |
5.06e-09 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 58.87 E-value: 5.06e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 6 FHFVNRfrlQAKKWLNRTALRfreqAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARA 85
Cdd:cd12115 2 HDLVEA---QAARTPDAIALV----CGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGA 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 86 VAVPIYATNTAKQVEYIVNDADIKILFVgdqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneae 165
Cdd:cd12115 75 AYVPLDPAYPPERLRFILEDAQARLVLT---------------------------------------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 166 fekrlnskQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLN-------AHDLA--LNVNEDDVSLS----FLPLSHifer 232
Cdd:cd12115 103 --------DPDDLAYVIYTSGSTGRPKGVAIEHRNAAAFLQwaaaafsAEELAgvLASTSICFDLSvfelFGPLAT---- 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 233 awiayvfhrGATNCYLEDTKHVRD----ALTTLKPTVMCAVPRFYEkiytavwdkVEKAPLHRRALfNWAirvGEqhyqi 308
Cdd:cd12115 171 ---------GGKVVLADNVLALPDlpaaAEVTLINTVPSAAAELLR---------HDALPASVRVV-NLA---GE----- 223
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 309 eqpsqwlrlqyALADKLVLtKLRALLGG-RIkmmpcggakleasigsffhsigINIklgYGMTETT--ATVSCWQDKGFN 385
Cdd:cd12115 224 -----------PLPRDLVQ-RLYARLQVeRV----------------------VNL---YGPSEDTtySTVAPVPPGASG 266
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 386 PNSIGTLMPN----------AEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFL------RTGDVGEMDSCGNLF 449
Cdd:cd12115 267 EVSIGRPLANtqayvldralQPVPLGVPGELYIGGAGVARGYLGRPGLTAERFLPDPFGpgarlyRTGDLVRWRPDGLLE 346
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 450 ITDRLKELMKTsNGKYIAPQYIEGKIGKDKFIEQIAVI----ADAKKYVSALIVP 500
Cdd:cd12115 347 FLGRADNQVKV-RGFRIELGEIEAALRSIPGVREAVVVaigdAAGERRLVAYIVA 400
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
181-459 |
2.91e-08 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 56.90 E-value: 2.91e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANLahQLNAHDLALNV--NEDDVSLSFLPLSHIFerawiayvfhrGATNCYLedtkhvrdaL 258
Cdd:PRK06814 798 ILFTSGSEGTPKGVVLSHRNL--LANRAQVAARIdfSPEDKVFNALPVFHSF-----------GLTGGLV---------L 855
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 259 TTLK--PTVMCAVPRFYEKIYTAVWDKvekaplhrralfNWAIRVGEQHY-----QIEQPSQWLRLQYALAdklvltklr 331
Cdd:PRK06814 856 PLLSgvKVFLYPSPLHYRIIPELIYDT------------NATILFGTDTFlngyaRYAHPYDFRSLRYVFA--------- 914
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 332 allggrikmmpcGGAKLEASIGSFF-HSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVK------IGEEN 404
Cdd:PRK06814 915 ------------GAEKVKEETRQTWmEKFGIRILEGYGVTETAPVIALNTPMHNKAGTVGRLLPGIEYRlepvpgIDEGG 982
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 EILVRGGMVMRGYYK--KP---EETakaftEDGFLRTGDVGEMDSCGNLFITDRLKELMK 459
Cdd:PRK06814 983 RLFVRGPNVMLGYLRaeNPgvlEPP-----ADGWYDTGDIVTIDEEGFITIKGRAKRFAK 1037
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
174-488 |
4.00e-08 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 55.72 E-value: 4.00e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 174 QLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLShiFERA-W-IAYVFHRGATnCYLEDT 251
Cdd:cd17652 91 TPDNLAYVIYTSGSTGRPKGVVVTHRGLANLAAAQIAAFDVGPGSRVLQFASPS--FDASvWeLLMALLAGAT-LVLAPA 167
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 252 ------KHVRDALT-------TLKPTVMCAVPrfyekiytavwdkvEKAPLHRRALfnwaIRVGEqhyqieqpsqwlrlq 318
Cdd:cd17652 168 eellpgEPLADLLRehrithvTLPPAALAALP--------------PDDLPDLRTL----VVAGE--------------- 214
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 319 yALADKLVltklRALLGGRiKMmpcggakleasigsffhsigINiklGYGMTETT--ATVS-CwqDKGFNPNSIGTLMPN 395
Cdd:cd17652 215 -ACPAELV----DRWAPGR-RM--------------------IN---AYGPTETTvcATMAgP--LPGGGVPPIGRPVPG 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 396 AEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGDVGEMDSCGNLFITDRLKELM 458
Cdd:cd17652 264 TRVYVldarlrpvppGVPGELYIAGAGLARGYLNRPGLTAERFVADPFgapgsrmYRTGDLARWRADGQLEFLGRADDQV 343
|
330 340 350
....*....|....*....|....*....|
gi 544685990 459 KTsNGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:cd17652 344 KI-RGFRIELGEVEAALTEHPGVAEAVVVV 372
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
360-487 |
4.07e-08 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 55.65 E-value: 4.07e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 360 GINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI-------GEENEILV-----RGGMVMRGYYKKPEETAKA 427
Cdd:cd05974 225 GLTIRDGYGQTETTALVGNSPGQPVKAGSMGRPLPGYRVALldpdgapATEGEVALdlgdtRPVGLMKGYAGDPDKTAHA 304
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 428 FtEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKyIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:cd05974 305 M-RGGYYRTGDIAMRDEDGYLTYVGRADDVFKSSDYR-ISPFELESVLIEHPAVAEAAVV 362
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
184-472 |
9.17e-08 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 55.00 E-value: 9.17e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 184 TSGTTGEPKGVMLDYANLAHQLNAHDLALNVN-EDDVSLSFLPLSHifERAWIAYV---FHRGATNCYLEDTKHVRDAL- 258
Cdd:PRK07768 160 TSGSTGSPKAVQITHGNLYANAEAMFVAAEFDvETDVMVSWLPLFH--DMGMVGFLtvpMYFGAELVKVTPMDFLRDPLl 237
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 259 -----TTLKPTVMCAvPRF-YekiytavwdkvekAPLHRRalfnwaIRVGEQHYQIEQPSqwlrLQYAL--ADKLVLTKL 330
Cdd:PRK07768 238 waeliSKYRGTMTAA-PNFaY-------------ALLARR------LRRQAKPGAFDLSS----LRFALngAEPIDPADV 293
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 331 RALL--GGRIKMMPcgGAKLEAsigsffhsiginiklgYGMTETTATVScwqdkgFNP---------------------- 386
Cdd:PRK07768 294 EDLLdaGARFGLRP--EAILPA----------------YGMAEATLAVS------FSPcgaglvvdevdadllaalrrav 349
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 387 ----------NSIGTLMPNAEVKIGEEN----------EILVRGGMVMRGyYKKPEETAKAFTEDGFLRTGDVGEMDSCG 446
Cdd:PRK07768 350 patkgntrrlATLGPPLPGLEVRVVDEDgqvlpprgvgVIELRGESVTPG-YLTMDGFIPAQDADGWLDTGDLGYLTEEG 428
|
330 340
....*....|....*....|....*.
gi 544685990 447 NLFITDRLKELMKTSnGKYIAPQYIE 472
Cdd:PRK07768 429 EVVVCGRVKDVIIMA-GRNIYPTDIE 453
|
|
| PRK05850 |
PRK05850 |
acyl-CoA synthetase; Validated |
391-458 |
1.01e-07 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235624 [Multi-domain] Cd Length: 578 Bit Score: 54.95 E-value: 1.01e-07
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 544685990 391 TLMPNAEVKIGEeneILVRGGMVMRGYYKKPEETAKAF----------TEDG-FLRTGDVGEMdSCGNLFITDRLKELM 458
Cdd:PRK05850 388 TCIECPAGTVGE---IWVHGDNVAAGYWQKPEETERTFgatlvdpspgTPEGpWLRTGDLGFI-SEGELFIVGRIKDLL 462
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
21-219 |
1.11e-07 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 54.90 E-value: 1.11e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRFREQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:PRK04319 59 DKVALRYLDASRKEKYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVR 138
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILfVGDQEQFDQVyqIANNCPQLMKIVAMKAnmHLRDLPNACYWEDFLNVVPNEAEFEkrlnSKQLSDLFT 180
Cdd:PRK04319 139 DRLEDSEAKVL-ITTPALLERK--PADDLPSLKHVLLVGE--DVEEGPGTLDFNALMEQASDEFDIE----WTDREDGAI 209
|
170 180 190 200
....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVM-LDYANLAHQLNAHdLALNVNEDDV 219
Cdd:PRK04319 210 LHYTSGSTGKPKGVLhVHNAMLQHYQTGK-YVLDLHEDDV 248
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
394-472 |
1.70e-07 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 54.23 E-value: 1.70e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 394 PNAEVKIGEEN-EILVRG--GMVM-------RGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNG 463
Cdd:PRK10946 361 PDDEVWVADADgNPLPQGevGRLMtrgpytfRGYYKSPQHNASAFDANGFYCSGDLVSIDPDGYITVVGREKDQI-NRGG 439
|
....*....
gi 544685990 464 KYIAPQYIE 472
Cdd:PRK10946 440 EKIAAEEIE 448
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
176-488 |
4.16e-07 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 52.79 E-value: 4.16e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 176 SDLFTLIYTSGTTGEPKGVMLDYANLAHQLNahDLA----LNVNEDDVSLSFlplshiferawIAYVFhrgatNCYLEDT 251
Cdd:cd17648 94 TDLAYAIYTSGTTGKPKGVLVEHGSVVNLRT--SLSeryfGRDNGDEAVLFF-----------SNYVF-----DFFVEQM 155
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 252 KhvrDALTT------LKPTVMCAVPRFYEKIYTavwDKVEkaplhrralfnwairvgeqhYQIEQPSQWLRLQYALADKL 325
Cdd:cd17648 156 T---LALLNgqklvvPPDEMRFDPDRFYAYINR---EKVT--------------------YLSGTPSVLQQYDLARLPHL 209
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 326 vltklrallggriKMMPCGGAKLEAS----IGSFFHSIGINiklGYGMTETTATVSCWQDKGFNP--NSIGTLMPNAE-- 397
Cdd:cd17648 210 -------------KRVDAAGEEFTAPvfekLRSRFAGLIIN---AYGPTETTVTNHKRFFPGDQRfdKSLGRPVRNTKcy 273
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 398 --------VKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGF--------------LRTGDVGEMDSCGNLFITDRlK 455
Cdd:cd17648 274 vlndamkrVPVGAVGELYLGGDGVARGYLNRPELTAERFLPNPFqteqerargrnarlYKTGDLVRWLPSGELEYLGR-N 352
|
330 340 350
....*....|....*....|....*....|...
gi 544685990 456 ELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:cd17648 353 DFQVKIRGQRIEPGEVEAALASYPGVRECAVVA 385
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
34-575 |
6.57e-07 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 52.86 E-value: 6.57e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 34 QEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILfV 113
Cdd:PRK12467 3119 QQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYVPLDPEYPRERLAYMIEDSGVKLL-L 3197
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 114 GDQEQFDQVYQIANNCPQLMKIVAMKAnmhlrdLPNACywedflnvvPNeaefekrlNSKQLSDLFTLIYTSGTTGEPKG 193
Cdd:PRK12467 3198 TQAHLLEQLPAPAGDTALTLDRLDLNG------YSENN---------PS--------TRVMGENLAYVIYTSGSTGKPKG 3254
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 194 VMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLShiFERAwiayvfhrgatncyledtkhVRDALTTLkptvMCAvprfy 273
Cdd:PRK12467 3255 VGVRHGALANHLCWIAEAYELDANDRVLLFMSFS--FDGA--------------------QERFLWTL----ICG----- 3303
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 274 ekiytavwdkvekAPLHRRALFNWAirvGEQHYQieqpsQWLRLQYALADkLVLTKLRALL-------GGRIKMMPCGGA 346
Cdd:PRK12467 3304 -------------GCLVVRDNDLWD---PEELWQ-----AIHAHRISIAC-FPPAYLQQFAedaggadCASLDIYVFGGE 3361
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 347 KL-EASIGSFF-HSIGINIKLGYGMTETTATVSCW---QDKGFNPNS--IGTLMPNAE----------VKIGEENEILVR 409
Cdd:PRK12467 3362 AVpPAAFEQVKrKLKPRGLTNGYGPTEAVVTVTLWkcgGDAVCEAPYapIGRPVAGRSiyvldgqlnpVPVGVAGELYIG 3441
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 410 GGMVMRGYYKKPEETAKAFTEDGFL-------RTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGKIGKDKFIE 482
Cdd:PRK12467 3442 GVGLARGYHQRPSLTAERFVADPFSgsggrlyRTGDLARYRADGVIEYLGRIDHQVKI-RGFRIELGEIEARLLQHPSVR 3520
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 483 QIAVIA---DAKKYVSALIVPcfdsleeyakqlNIKYQD-RIELIKHsdiiqmferriheLQKELPSFEQVKRFTLLPQA 558
Cdd:PRK12467 3521 EAVVLArdgAGGKQLVAYVVP------------ADPQGDwRETLRDH-------------LAASLPDYMVPAQLLVLAAM 3575
|
570
....*....|....*..
gi 544685990 559 fstameEITPTLKLRRK 575
Cdd:PRK12467 3576 ------PLGPNGKVDRK 3586
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
61-500 |
1.36e-06 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 51.25 E-value: 1.36e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 61 DKIGIFannMPRWTI---ADFGA-MQARAVAVPIYaTNTAKQVEYIVNDADIKILFVgdQEQFDQVYQIANnCPQLMKiv 136
Cdd:PRK08043 256 ERIGLM---LPNATIsaaVIFGAsLRRRIPAMMNY-TAGVKGLTSAITAAEIKTIFT--SRQFLDKGKLWH-LPEQLT-- 326
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 137 amKAN-MHLRDLPNACYWEDFLNV-----VPNEAEFekrlnSKQLSDLFTLIYTSGTTGEPKGVMLDYANLahqlnahdL 210
Cdd:PRK08043 327 --QVRwVYLEDLKDDVTTADKLWIfahllMPRLAQV-----KQQPEDAALILFTSGSEGHPKGVVHSHKSL--------L 391
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 211 AlNVNE---------DDVSLSFLPLSHIFerawiayvfhrGATNCYLedTKHVRDALTTLKPTvmcavPRFYEKIYTAVW 281
Cdd:PRK08043 392 A-NVEQiktiadftpNDRFMSALPLFHSF-----------GLTVGLF--TPLLTGAEVFLYPS-----PLHYRIVPELVY 452
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 282 DKvekaplHRRALFNWAIRVGeqHY-QIEQPSQWLRLQYALAdklvltklrallggrikmmpcGGAKLEASIGS-FFHSI 359
Cdd:PRK08043 453 DR------NCTVLFGTSTFLG--NYaRFANPYDFARLRYVVA---------------------GAEKLQESTKQlWQDKF 503
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 360 GINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVK------IGEENEILVRGGMVMRGYYK--KP-------EET 424
Cdd:PRK08043 504 GLRILEGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARllsvpgIEQGGRLQLKGPNIMNGYLRveKPgvlevptAEN 583
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 425 AKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKtsngkyiapqyIEGKIGKDKFIEQIAVIADAKKYVSALIVP 500
Cdd:PRK08043 584 ARGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAK-----------IAGEMVSLEMVEQLALGVSPDKQHATAIKS 648
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
397-472 |
1.82e-06 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 50.84 E-value: 1.82e-06
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 397 EVKIGEENEILVRGGMVMRgYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRlKELMKTSNGKYIAPQYIE 472
Cdd:cd05929 316 EVPPGEIGEVYFANGPGFE-YTNDPEKTAAARNEGGWSTLGDVGYLDEDGYLYLTDR-RSDMIISGGVNIYPQEIE 389
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
11-228 |
1.89e-06 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 50.64 E-value: 1.89e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 11 RFRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI 90
Cdd:PRK08279 42 VFEEAAARHPDRPALLFEDQ----SISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVALL 117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 91 YATNTAKQVEYIVNDADIKILFVGdqEQFDQVYQIANNCPQLMKIVAMKANMHLRDLPNacyWEDFLNVVPNEAEFEKRL 170
Cdd:PRK08279 118 NTQQRGAVLAHSLNLVDAKHLIVG--EELVEAFEEARADLARPPRLWVAGGDTLDDPEG---YEDLAAAAAGAPTTNPAS 192
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 171 NSK-QLSDLFTLIYTSGTTGEPKGV-------MLDYANLAHQLNAhdlalnvNEDDVSLSFLPLSH 228
Cdd:PRK08279 193 RSGvTAKDTAFYIYTSGTTGLPKAAvmshmrwLKAMGGFGGLLRL-------TPDDVLYCCLPLYH 251
|
|
| PRK12476 |
PRK12476 |
putative fatty-acid--CoA ligase; Provisional |
385-500 |
1.92e-06 |
|
putative fatty-acid--CoA ligase; Provisional
Pssm-ID: 171527 [Multi-domain] Cd Length: 612 Bit Score: 50.90 E-value: 1.92e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 385 NPNSiGTLMPNAEVkigeeNEILVRGGMVMRGYYKKPEETAKAF----------------TEDG--FLRTGDVG-EMDsc 445
Cdd:PRK12476 417 DPDT-GAELPDGEV-----GEIWLHGDNIGRGYWGRPEETERTFgaklqsrlaegshadgAADDgtWLRTGDLGvYLD-- 488
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 446 GNLFITDRLKELMkTSNGKYIAPQYIEgkigkdkfieqiAVIADA-----KKYVSALIVP 500
Cdd:PRK12476 489 GELYITGRIADLI-VIDGRNHYPQDIE------------ATVAEAspmvrRGYVTAFTVP 535
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
367-472 |
2.75e-06 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 50.31 E-value: 2.75e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 367 YGMTE-TTATVSCWQDKGFNPNSIGTLMPNAEVKIGEEN----------EILVRGGMVMRGYY--KKPEetakafTEDGF 433
Cdd:PRK07788 355 YGSTEvAFATIATPEDLAEAPGTVGRPPKGVTVKILDENgnevprgvvgRIFVGNGFPFEGYTdgRDKQ------IIDGL 428
|
90 100 110
....*....|....*....|....*....|....*....
gi 544685990 434 LRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIE 472
Cdd:PRK07788 429 LSSGDVGYFDEDGLLFVDGRDDD-MIVSGGENVFPAEVE 466
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
181-465 |
3.57e-06 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 49.77 E-value: 3.57e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAW-----IAYVFHRGATNCyleDTKHVR 255
Cdd:cd05910 90 ILFTSGSTGTPKGVVYRHGTFAAQIDALRQLYGIRPGEVDLATFPLFALFGPALgltsvIPDMDPTRPARA---DPQKLV 166
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 256 DALTTLKPTVMCAVPrfyekiytAVWDKVEkaplhrralfnwaiRVGEQHyQIEQPSQWLRLQY-ALADKLVLTKLRALL 334
Cdd:cd05910 167 GAIRQYGVSIVFGSP--------ALLERVA--------------RYCAQH-GITLPSLRRVLSAgAPVPIALAARLRKML 223
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 335 GGRIKMMPCGGAKlEA----SIGSffHSIginiklgygMTETTATVScwQDKGfnpNSIGTLMPNAEVKI---------- 400
Cdd:cd05910 224 SDEAEILTPYGAT-EAlpvsSIGS--REL---------LATTTAATS--GGAG---TCVGRPIPGVRVRIieiddepiae 286
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 401 ---------GEENEILVRGGMVMRGYYKKPEETAKAFTED---GFL-RTGDVGEMDSCGNLFITDRLKELMKTSNGKY 465
Cdd:cd05910 287 wddtlelprGEIGEITVTGPTVTPTYVNRPVATALAKIDDnseGFWhRMGDLGYLDDEGRLWFCGRKAHRVITTGGTL 364
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
170-459 |
4.06e-06 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 49.81 E-value: 4.06e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 170 LNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHiferawiAYVFHrgatNCYLE 249
Cdd:PRK06334 177 VSDKDPEDVAVILFTSGTEKLPKGVPLTHANLLANQRACLKFFSPKEDDVMMSFLPPFH-------AYGFN----SCTLF 245
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 dtkhvrdALTTLKPTVMCAVPRFYEKIYTAVwDKVEKAPLHRRALFNWAIrvgeqhyqieqpsqwlrLQYALADKLVLTK 329
Cdd:PRK06334 246 -------PLLSGVPVVFAYNPLYPKKIVEMI-DEAKVTFLGSTPVFFDYI-----------------LKTAKKQESCLPS 300
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 330 LR-ALLGGRIkmmpCGGAKLEASIGSFFHsigINIKLGYGMTETTATVSC-WQDKGFNPNSIGTLMPNAEVKI------- 400
Cdd:PRK06334 301 LRfVVIGGDA----FKDSLYQEALKTFPH---IQLRQGYGTTECSPVITInTVNSPKHESCVGMPIRGMDVLIvseetkv 373
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 401 ----GEENEILVRGGMVMRGYYKkpEETAKAFTE---DGFLRTGDVGEMDSCGNLFITDRLKELMK 459
Cdd:PRK06334 374 pvssGETGLVLTRGTSLFSGYLG--EDFGQGFVElggETWYVTGDLGYVDRHGELFLKGRLSRFVK 437
|
|
| PRK07769 |
PRK07769 |
long-chain-fatty-acid--CoA ligase; Validated |
34-500 |
4.12e-06 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 181109 [Multi-domain] Cd Length: 631 Bit Score: 49.73 E-value: 4.12e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 34 QEMSWQTFQQEIdRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE---YIVNDADIKI 110
Cdd:PRK07769 54 RDLTWSQFGARN-RAVGARLQQVTKPGDRVAILAPQNLDYLIAFFGALYAGRIAVPLFDPAEPGHVGrlhAVLDDCTPSA 132
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 111 LFVGDqEQFDQVYQIANNCP--QLMKIVAMKAnmhlrdLPNACyWEDFLNVVPNEaefekrlnskqlSDLFTLIYTSGTT 188
Cdd:PRK07769 133 ILTTT-DSAEGVRKFFRARPakERPRVIAVDA------VPDEV-GATWVPPEANE------------DTIAYLQYTSGST 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 189 GEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHifERAWIAYVFHR--GATNCYLEDTKHVRDALTTLKPtvM 266
Cdd:PRK07769 193 RIPAGVQITHLNLPTNVLQVIDALEGQEGDRGVSWLPFFH--DMGLITVLLPAllGHYITFMSPAAFVRRPGRWIRE--L 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 267 CAVPRFYEKIYTAvwdkvekAPlhrralfNWAI---------RVGEQhyqieqpsqwlrlqyaladKLVLTKLRALLGGR 337
Cdd:PRK07769 269 ARKPGGTGGTFSA-------AP-------NFAFehaaarglpKDGEP-------------------PLDLSNVKGLLNGS 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 338 IKMMPcggakleASIGSFFHSIG------INIKLGYGMTETTATVSC--WQDKgfnPNSI---------GTLM------P 394
Cdd:PRK07769 316 EPVSP-------ASMRKFNEAFApyglppTAIKPSYGMAEATLFVSTtpMDEE---PTVIyvdrdelnaGRFVevpadaP 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 395 NA------------------------EVKIGEENEILVRGGMVMRGYYKKPEETAKAF----------------TEDG-F 433
Cdd:PRK07769 386 NAvaqvsagkvgvsewavivdpetasELPDGQIGEIWLHGNNIGTGYWGKPEETAATFqnilksrlseshaegaPDDAlW 465
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 434 LRTGDVGE-MDscGNLFITDRLKELMkTSNGKYIAPQYIEgkigkdkFIEQIAVIADAKKYVSALIVP 500
Cdd:PRK07769 466 VRTGDYGVyFD--GELYITGRVKDLV-IIDGRNHYPQDLE-------YTAQEATKALRTGYVAAFSVP 523
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
22-488 |
1.42e-05 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 48.10 E-value: 1.42e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 22 RTALRFREQaQWqemSWQTFQQEIDRFSYALIAQHI-DIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:PRK13388 17 TIAVRYGDR-TW---TWREVLAEAAARAAALIALADpDRPLHVGVLLGNTPEMLFWLAAAALGGYVLVGLNTTRRGAALA 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILfVGDQEqfdqvyqianNCPQLMKIvamkanmhlrDLPNacywEDFLNVvpNEAEFEKRLNSKQ------ 174
Cdd:PRK13388 93 ADIRRADCQLL-VTDAE----------HRPLLDGL----------DLPG----VRVLDV--DTPAYAELVAAAGaltphr 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 175 ---LSDLFTLIYTSGTTGEPKGVMLDYANLAhqLNAHDLA--LNVNEDDVSLSFLPLSH---IFerAWIAYVFHRGATnc 246
Cdd:PRK13388 146 evdAMDPFMLIFTSGTTGAPKAVRCSHGRLA--FAGRALTerFGLTRDDVCYVSMPLFHsnaVM--AGWAPAVASGAA-- 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 247 yledtkhvrdalttlkptvMCAVPRFYEkiyTAVWDKVekaplhRR---ALFNWairVGEQHYQI----EQPSQwlrlqy 319
Cdd:PRK13388 220 -------------------VALPAKFSA---SGFLDDV------RRygaTYFNY---VGKPLAYIlatpERPDD------ 262
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 320 alADklvlTKLRALLGGrikmmpcggaklEAS---IGSFFHSIGINIKLGYGMTETTATVScwQDKGFNPNSIG------ 390
Cdd:PRK13388 263 --AD----NPLRVAFGN------------EASprdIAEFSRRFGCQVEDGYGSSEGAVIVV--REPGTPPGSIGrgapgv 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 391 ------TLMP-------------NAEVKIGEeneiLV--RGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLF 449
Cdd:PRK13388 323 aiynpeTLTEcavarfdahgallNADEAIGE----LVntAGAGFFEGYYNNPEATAERM-RHGMYWSGDLAYRDADGWIY 397
|
490 500 510
....*....|....*....|....*....|....*....
gi 544685990 450 ITDRLKELMKTsNGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:PRK13388 398 FAGRTADWMRV-DGENLSAAPIERILLRHPAINRVAVYA 435
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
15-487 |
2.42e-05 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 47.86 E-value: 2.42e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 15 QAKKWLNRTALrfreqaQWQEMS--WQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYA 92
Cdd:PRK05691 1140 QARQTPERIAL------VWDGGSldYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLDP 1213
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 93 TNTAKQVEYIVNDADIKILfVGDQEQFDQVYQIANNCPqlmkiVAMkANMHLRDLPNAcywedflnvVPNEAefekrLNS 172
Cdd:PRK05691 1214 DYPAERLAYMLADSGVELL-LTQSHLLERLPQAEGVSA-----IAL-DSLHLDSWPSQ---------APGLH-----LHG 1272
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 173 KQLSdlfTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLShiferawiayvFHRGATNCYLedtk 252
Cdd:PRK05691 1273 DNLA---YVIYTSGSTGQPKGVGNTHAALAERLQWMQATYALDDSDVLMQKAPIS-----------FDVSVWECFW---- 1334
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 253 hvrdALTTLKPTVMCAVPRfyekiytavwdkvekaplHR--RALFNWAIRVGEQHYQIEQPSQWLRLQYALADKlvLTKL 330
Cdd:PRK05691 1335 ----PLITGCRLVLAGPGE------------------HRdpQRIAELVQQYGVTTLHFVPPLLQLFIDEPLAAA--CTSL 1390
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 331 RALLggrikmmpCGGAKLEASIGSFFHSIGINIKLG--YGMTETTATVSCWQ----DKGFNPnsIGTLMPNA--EVKIGE 402
Cdd:PRK05691 1391 RRLF--------SGGEALPAELRNRVLQRLPQVQLHnrYGPTETAINVTHWQcqaeDGERSP--IGRPLGNVlcRVLDAE 1460
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 403 EN--------EILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIA 467
Cdd:PRK05691 1461 LNllppgvagELCIGGAGLARGYLGRPALTAERFVPDPLgedgarlYRTGDRARWNADGALEYLGRLDQQVKL-RGFRVE 1539
|
490 500
....*....|....*....|
gi 544685990 468 PQYIEGKIGKDKFIEQIAVI 487
Cdd:PRK05691 1540 PEEIQARLLAQPGVAQAAVL 1559
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
179-493 |
2.43e-05 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 47.08 E-value: 2.43e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 179 FTLIYTSGTTGEPKGVMLDYANLAH--QLNAHDLALNvNEDDVSLSFLPLSHIFERAWIAyVFHRGATNCYLED--TKHV 254
Cdd:PRK07638 146 FYMGFTSGSTGKPKAFLRAQQSWLHsfDCNVHDFHMK-REDSVLIAGTLVHSLFLYGAIS-TLYVGQTVHLMRKfiPNQV 223
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 255 RDALTTLKPTVMCAVPRFYEKIYtavwdKVEKAPlhrralfnwairvgeqhyqiEQPsqwlrlqyalaDKLVLTklrall 334
Cdd:PRK07638 224 LDKLETENISVMYTVPTMLESLY-----KENRVI--------------------ENK-----------MKIISS------ 261
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 335 ggrikmmpcgGAKLEASIGSFFHSIGINIKLG--YGMTE---TTATVScwQDKGFNPNSIGTLMPNAEVKI--------- 400
Cdd:PRK07638 262 ----------GAKWEAEAKEKIKNIFPYAKLYefYGASElsfVTALVD--EESERRPNSVGRPFHNVQVRIcneageevq 329
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -GEENEILVRGGMVMRGYyKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDK 479
Cdd:PRK07638 330 kGEIGTVYVKSPQFFMGY-IIGGVLARELNADGWMTVRDVGYEDEEGFIYIVGREKN-MILFGGINIFPEEIESVLHEHP 407
|
330
....*....|....
gi 544685990 480 FIEQIAVIADAKKY 493
Cdd:PRK07638 408 AVDEIVVIGVPDSY 421
|
|
| Ac_CoA_lig_AcsA |
TIGR02188 |
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ... |
21-205 |
6.64e-05 |
|
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.
Pssm-ID: 274022 [Multi-domain] Cd Length: 626 Bit Score: 45.70 E-value: 6.64e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 21 NRTALRFR--EQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIAdfgaMQA--R--AVAVPIYATN 94
Cdd:TIGR02188 72 DKVAIIWEgdEPGEVRKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIA----MLAcaRigAIHSVVFGGF 147
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 95 TAKQVEYIVNDADIKILFVGDqEQF---------DQVYQIANNCPQLMK--IVAMKANMHL------RDLpnacYWEDFL 157
Cdd:TIGR02188 148 SAEALADRINDAGAKLVITAD-EGLrggkviplkAIVDEALEKCPVSVEhvLVVRRTGNPVvpwvegRDV----WWHDLM 222
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....
gi 544685990 158 NVVPNEAEFEKrLNSKQLsdLFTLiYTSGTTGEPKGVM------LDYANLAHQL 205
Cdd:TIGR02188 223 AKASAYCEPEP-MDSEDP--LFIL-YTSGSTGKPKGVLhttggyLLYAAMTMKY 272
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
327-487 |
8.82e-05 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 45.37 E-value: 8.82e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 327 LTKLRALLGGRIKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWqdkgfnPNSIGTLMPNAEVKIGEEN-- 404
Cdd:PRK13383 291 LPQLRVVMSSGDRLDPTLGQRFMDTYGDILYNGYGSTEVGIGALATPADLRDA------PETVGKPVAGCPVRILDRNnr 364
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 --------EILVRGGMVMRGYykkPEETAKAFTeDGFLRTGDVGEMDSCGNLFITDRlKELMKTSNGKYIAPQYIEGKIG 476
Cdd:PRK13383 365 pvgprvtgRIFVGGELAGTRY---TDGGGKAVV-DGMTSTGDMGYLDNAGRLFIVGR-EDDMIISGGENVYPRAVENALA 439
|
170
....*....|.
gi 544685990 477 KDKFIEQIAVI 487
Cdd:PRK13383 440 AHPAVADNAVI 450
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
174-228 |
1.24e-04 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 45.06 E-value: 1.24e-04
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 174 QLSDLFTLIYTSGTTGEPKGVMLDYANLA---HQLNAHdlaLNVNEDDVSLSFLPLSH 228
Cdd:PRK07867 150 DPDDLFMLIFTSGTSGDPKAVRCTHRKVAsagVMLAQR---FGLGPDDVCYVSMPLFH 204
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
417-516 |
2.37e-04 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 43.84 E-value: 2.37e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 417 YYKKPEETAKA-FTEDGFLRT-GDVGEMDSCGNLFITDRlKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIA----DA 490
Cdd:PRK13390 362 YLNDPEKTAAAqHPAHPFWTTvGDLGSVDEDGYLYLADR-KSFMIISGGVNIYPQETENALTMHPAVHDVAVIGvpdpEM 440
|
90 100
....*....|....*....|....*...
gi 544685990 491 KKYVSALI--VPCFDSLEEYAKQLnIKY 516
Cdd:PRK13390 441 GEQVKAVIqlVEGIRGSDELAREL-IDY 467
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
43-219 |
3.57e-04 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 43.32 E-value: 3.57e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 43 QEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIAdfgaMQA--R--AVAVPIYATNTAKQVEYIVNDADIKILFVGDQ-- 116
Cdd:cd05966 92 REVCRFANVLKSLGVKKGDRVAIYMPMIPELVIA----MLAcaRigAVHSVVFAGFSAESLADRINDAQCKLVITADGgy 167
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 117 --EQFDQVYQIAN----NCPQLMK-IVAMKAN-----MHLRDLpnacYWEDFLNVVPNEAEFEkRLNSKqlsD-LFTLiY 183
Cdd:cd05966 168 rgGKVIPLKEIVDealeKCPSVEKvLVVKRTGgevpmTEGRDL----WWHDLMAKQSPECEPE-WMDSE---DpLFIL-Y 238
|
170 180 190 200
....*....|....*....|....*....|....*....|..
gi 544685990 184 TSGTTGEPKGVM------LDYANLAHQlnahdLALNVNEDDV 219
Cdd:cd05966 239 TSGSTGKPKGVVhttggyLLYAATTFK-----YVFDYHPDDI 275
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
136-204 |
5.83e-04 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 42.82 E-value: 5.83e-04
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 136 VAMKANmhlRDLpnacYWEDFLNVVPNEAEFEkRLNSKQLsdLFTLiYTSGTTGEPKGVM------LDYANLAHQ 204
Cdd:PRK00174 216 VDWVEG---RDL----WWHELVAGASDECEPE-PMDAEDP--LFIL-YTSGSTGKPKGVLhttggyLVYAAMTMK 279
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
34-195 |
8.71e-04 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 42.26 E-value: 8.71e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 34 QEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIA----------------DFGAmqaRAVAvpiyatNTAK 97
Cdd:cd05943 97 TEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAmlatasigaiwsscspDFGV---PGVL------DRFG 167
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 98 QVEyivndadIKILFVGDQEQF--------DQVYQIANNCPQLMKIVAM-----KANMHLRDLPNACYWEDFLNVVPN-E 163
Cdd:cd05943 168 QIE-------PKVLFAVDAYTYngkrhdvrEKVAELVKGLPSLLAVVVVpytvaAGQPDLSKIAKALTLEDFLATGAAgE 240
|
170 180 190
....*....|....*....|....*....|..
gi 544685990 164 AEFEkRLNSKQLsdLFTLiYTSGTTGEPKGVM 195
Cdd:cd05943 241 LEFE-PLPFDHP--LYIL-YSSGTTGLPKCIV 268
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
181-438 |
1.86e-03 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 41.69 E-value: 1.86e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLShiFERAwiayvfhrgatncyledTKHVRDALTT 260
Cdd:PRK05691 2338 LIYTSGSTGKPKGVVVSHGEIAMHCQAVIERFGMRADDCELHFYSIN--FDAA-----------------SERLLVPLLC 2398
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 261 LKPTVMCAvprfyekiyTAVWDKVEKAPLHRralfnwairvgEQHYQI--------EQPSQWLRLQYA-LADKLVLTKLR 331
Cdd:PRK05691 2399 GARVVLRA---------QGQWGAEEICQLIR-----------EQQVSIlgftpsygSQLAQWLAGQGEqLPVRMCITGGE 2458
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 332 ALLGGRIKMMPCGGAKleasiGSFFHSiginiklgYGMTETT-------ATVSCWQDKGFNPnsIGTLMPN--------- 395
Cdd:PRK05691 2459 ALTGEHLQRIRQAFAP-----QLFFNA--------YGPTETVvmplaclAPEQLEEGAASVP--IGRVVGArvayildad 2523
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|.
gi 544685990 396 -AEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGD 438
Cdd:PRK05691 2524 lALVPQGATGELYVGGAGLAQGYHDRPGLTAERFVADPFaadggrlYRTGD 2574
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
163-228 |
2.13e-03 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 40.79 E-value: 2.13e-03
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990 163 EAEFEKRLNSKQLSDLFTLI-YTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSH 228
Cdd:PRK08308 87 ESDFTKLEAVNYLAEEPSLLqYSSGTTGEPKLIRRSWTEIDREIEAYNEALNCEQDETPIVACPVTH 153
|
|
| A_NRPS_alphaAR |
cd17647 |
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ... |
361-447 |
4.26e-03 |
|
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.
Pssm-ID: 341302 [Multi-domain] Cd Length: 520 Bit Score: 39.81 E-value: 4.26e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 361 INIKLGYGMTETTATVS------CWQDKGFNPN-----SIGTLMPNAEV------------KIGEENEILVRGGMVMRGY 417
Cdd:cd17647 250 VRIVNMYGTTETQRAVSyfevpsRSSDPTFLKNlkdvmPAGRGMLNVQLlvvnrndrtqicGIGEVGEIYVRAGGLAEGY 329
|
90 100 110
....*....|....*....|....*....|
gi 544685990 418 YKKPEETAKAFTEDGFLRTGDVGEMDSCGN 447
Cdd:cd17647 330 RGLPELNKEKFVNNWFVEPDHWNYLDKDNN 359
|
|
|