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Conserved domains on  [gi|544685990|ref|WP_021117760|]
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long-chain fatty acid--CoA ligase [Glaesserella parasuis]

Protein Classification

AMP-dependent synthetase/ligase( domain architecture ID 11436967)

AMP-dependent synthetase/ligase such as long-chain fatty acid--CoA ligase, which catalyzes the activation of long-chain fatty acids (over C12) as acyl-CoA prior to fatty acid elongation

CATH:  3.30.300.30
EC:  6.2.1.-
Gene Ontology:  GO:0005524|GO:0015645
PubMed:  11255012|9373621
SCOP:  4002922

Graphical summary

 Zoom to residue level

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List of domain hits

Name Accession Description Interval E-value
FAA1 COG1022
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
7-592 0e+00

Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];


:

Pssm-ID: 440645 [Multi-domain]  Cd Length: 603  Bit Score: 830.51  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   7 HFVNRFRLQAKKWLNRTALRFREQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAV 86
Cdd:COG1022   12 TLPDLLRRRAARFPDRVALREKEDGIWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAV 91
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  87 AVPIYATNTAKQVEYIVNDADIKILFVGDQEQFDQVYQIANNCPQLMKIVAMKAnMHLRDLPNACYWEDFLNV---VPNE 163
Cdd:COG1022   92 TVPIYPTSSAEEVAYILNDSGAKVLFVEDQEQLDKLLEVRDELPSLRHIVVLDP-RGLRDDPRLLSLDELLALgreVADP 170
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 164 AEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGA 243
Cdd:COG1022  171 AELEARRAAVKPDDLATIIYTSGTTGRPKGVMLTHRNLLSNARALLERLPLGPGDRTLSFLPLAHVFERTVSYYALAAGA 250
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 244 TNCYLEDTKHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVGEQH----YQIEQPSQWLRLQY 319
Cdd:COG1022  251 TVAFAESPDTLAEDLREVKPTFMLAVPRVWEKVYAGIQAKAEEAGGLKRKLFRWALAVGRRYararLAGKSPSLLLRLKH 330
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 320 ALADKLVLTKLRALLGGRIKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVK 399
Cdd:COG1022  331 ALADKLVFSKLREALGGRLRFAVSGGAALGPELARFFRALGIPVLEGYGLTETSPVITVNRPGDNRIGTVGPPLPGVEVK 410
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 400 IGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDK 479
Cdd:COG1022  411 IAEDGEILVRGPNVMKGYYKNPEATAEAFDADGWLHTGDIGELDEDGFLRITGRKKDLIVTSGGKNVAPQPIENALKASP 490
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 480 FIEQIAVIADAKKYVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFERRIHELQKELPSFEQVKRFTLLPQAF 559
Cdd:COG1022  491 LIEQAVVVGDGRPFLAALIVPDFEALGEWAEENGLPYTSYAELAQDPEVRALIQEEVDRANAGLSRAEQIKRFRLLPKEF 570
                        570       580       590
                 ....*....|....*....|....*....|...
gi 544685990 560 STAMEEITPTLKLRRKVIMQRYREQIEEMYNER 592
Cdd:COG1022  571 TIENGELTPTLKLKRKVILEKYADLIEALYAGA 603
 
Name Accession Description Interval E-value
FAA1 COG1022
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
7-592 0e+00

Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];


Pssm-ID: 440645 [Multi-domain]  Cd Length: 603  Bit Score: 830.51  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   7 HFVNRFRLQAKKWLNRTALRFREQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAV 86
Cdd:COG1022   12 TLPDLLRRRAARFPDRVALREKEDGIWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAV 91
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  87 AVPIYATNTAKQVEYIVNDADIKILFVGDQEQFDQVYQIANNCPQLMKIVAMKAnMHLRDLPNACYWEDFLNV---VPNE 163
Cdd:COG1022   92 TVPIYPTSSAEEVAYILNDSGAKVLFVEDQEQLDKLLEVRDELPSLRHIVVLDP-RGLRDDPRLLSLDELLALgreVADP 170
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 164 AEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGA 243
Cdd:COG1022  171 AELEARRAAVKPDDLATIIYTSGTTGRPKGVMLTHRNLLSNARALLERLPLGPGDRTLSFLPLAHVFERTVSYYALAAGA 250
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 244 TNCYLEDTKHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVGEQH----YQIEQPSQWLRLQY 319
Cdd:COG1022  251 TVAFAESPDTLAEDLREVKPTFMLAVPRVWEKVYAGIQAKAEEAGGLKRKLFRWALAVGRRYararLAGKSPSLLLRLKH 330
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 320 ALADKLVLTKLRALLGGRIKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVK 399
Cdd:COG1022  331 ALADKLVFSKLREALGGRLRFAVSGGAALGPELARFFRALGIPVLEGYGLTETSPVITVNRPGDNRIGTVGPPLPGVEVK 410
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 400 IGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDK 479
Cdd:COG1022  411 IAEDGEILVRGPNVMKGYYKNPEATAEAFDADGWLHTGDIGELDEDGFLRITGRKKDLIVTSGGKNVAPQPIENALKASP 490
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 480 FIEQIAVIADAKKYVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFERRIHELQKELPSFEQVKRFTLLPQAF 559
Cdd:COG1022  491 LIEQAVVVGDGRPFLAALIVPDFEALGEWAEENGLPYTSYAELAQDPEVRALIQEEVDRANAGLSRAEQIKRFRLLPKEF 570
                        570       580       590
                 ....*....|....*....|....*....|...
gi 544685990 560 STAMEEITPTLKLRRKVIMQRYREQIEEMYNER 592
Cdd:COG1022  571 TIENGELTPTLKLKRKVILEKYADLIEALYAGA 603
VL_LC_FACS_like cd05907
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ...
32-577 0e+00

Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341233 [Multi-domain]  Cd Length: 452  Bit Score: 583.02  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  32 QWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKIL 111
Cdd:cd05907    2 VWQPITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKAL 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 112 FVGDqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqLSDLFTLIYTSGTTGEP 191
Cdd:cd05907   82 FVED-----------------------------------------------------------PDDLATIIYTSGTTGRP 102
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 192 KGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFE-RAWIAYVFHRGATNCYLEDTKHVRDALTTLKPTVMCAVP 270
Cdd:cd05907  103 KGVMLSHRNILSNALALAERLPATEGDRHLSFLPLAHVFErRAGLYVPLLAGARIYFASSAETLLDDLSEVRPTVFLAVP 182
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 RFYEKIYTAVwdKVEKAPLHRRALFNWAIrvgeqhyqieqpsqwlrlqyaladklvltklrallGGRIKMMPCGGAKLEA 350
Cdd:cd05907  183 RVWEKVYAAI--KVKAVPGLKRKLFDLAV-----------------------------------GGRLRFAASGGAPLPA 225
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 351 SIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTE 430
Cdd:cd05907  226 ELLHFFRALGIPVYEGYGLTETSAVVTLNPPGDNRIGTVGKPLPGVEVRIADDGEILVRGPNVMLGYYKNPEATAEALDA 305
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 431 DGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEEYAK 510
Cdd:cd05907  306 DGWLHTGDLGEIDEDGFLHITGRKKDLIITSGGKNISPEPIENALKASPLISQAVVIGDGRPFLVALIVPDPEALEAWAE 385
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990 511 QLNIKYQDRIELIKHSDIIQMFERRIHELQKELPSFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVI 577
Cdd:cd05907  386 EHGIAYTDVAELAANPAVRAEIEAAVEAANARLSRYEQIKKFLLLPEPFTIENGELTPTLKLKRPVI 452
AMP-binding pfam00501
AMP-binding enzyme;
12-461 1.01e-97

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 304.24  E-value: 1.01e-97
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   12 FRLQAKKWLNRTALRFREqaqWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:pfam00501   1 LERQAARTPDKTALEVGE---GRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLN 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   92 ATNTAKQVEYIVNDADIKILFVGDQEQFDQVYQIANNCPQLMKIVAMKANMHLRDlpnacywEDFLNVVPNEAEFEKRLN 171
Cdd:pfam00501  78 PRLPAEELAYILEDSGAKVLITDDALKLEELLEALGKLEVVKLVLVLDRDPVLKE-------EPLPEEAKPADVPPPPPP 150
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  172 SKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAH----DLALNVNEDDVSLSFLPLSHIFERAWIAY-VFHRGATNC 246
Cdd:pfam00501 151 PPDPDDLAYIIYTSGTTGKPKGVMLTHRNLVANVLSIkrvrPRGFGLGPDDRVLSTLPLFHDFGLSLGLLgPLLAGATVV 230
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  247 YLE-----DTKHVRDALTTLKPTVMCAVPRFYEKIytavwdkVEKAPLHRRALfnwairvgeqhyqieqpsqwlrlqyal 321
Cdd:pfam00501 231 LPPgfpalDPAALLELIERYKVTVLYGVPTLLNML-------LEAGAPKRALL--------------------------- 276
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  322 adklvltklrallgGRIKMMPCGGAKLEASIGSFFHSIGIN-IKLGYGMTETTATVSC---WQDKGFNPNSIGTLMPNAE 397
Cdd:pfam00501 277 --------------SSLRLVLSGGAPLPPELARRFRELFGGaLVNGYGLTETTGVVTTplpLDEDLRSLGSVGRPLPGTE 342
                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990  398 VKI-----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTS 461
Cdd:pfam00501 343 VKIvddetgepvppGEPGELCVRGPGVMKGYLNDPELTAEAFDEDGWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
PLN02736 PLN02736
long-chain acyl-CoA synthetase
26-591 6.92e-93

long-chain acyl-CoA synthetase


Pssm-ID: 178337 [Multi-domain]  Cd Length: 651  Bit Score: 298.94  E-value: 6.92e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  26 RFREQAQWQEMSWQTFQQEIDR---FSYALIAQHIDIQDKIGIFANNMPRWTIADFgAMQARA-VAVPIYATNTAKQVEY 101
Cdd:PLN02736  66 RIRVDGTVGEYKWMTYGEAGTArtaIGSGLVQHGIPKGACVGLYFINRPEWLIVDH-ACSAYSyVSVPLYDTLGPDAVKF 144
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 102 IVNDADIKILFVGDQeQFDQVYQIANNCPQLMKIVAMK-ANMHLRDLPNACYWEdflnVVP-NEAEFEKRLNSKQL---- 175
Cdd:PLN02736 145 IVNHAEVAAIFCVPQ-TLNTLLSCLSEIPSVRLIVVVGgADEPLPSLPSGTGVE----IVTySKLLAQGRSSPQPFrppk 219
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 176 -SDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERA-WIAYVFHRGATNCYLEDTKH 253
Cdd:PLN02736 220 pEDVATICYTSGTTGTPKGVVLTHGNLIANVAGSSLSTKFYPSDVHISYLPLAHIYERVnQIVMLHYGVAVGFYQGDNLK 299
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 254 VRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVGEQhyQIEQ---PSqwlrlqyALADKLVLTKL 330
Cdd:PLN02736 300 LMDDLAALRPTIFCSVPRLYNRIYDGITNAVKESGGLKERLFNAAYNAKKQ--ALENgknPS-------PMWDRLVFNKI 370
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 331 RALLGGRIKMMPCGGAKLEASIGSFFH-SIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVK---IGEEN-- 404
Cdd:PLN02736 371 KAKLGGRVRFMSSGASPLSPDVMEFLRiCFGGRVLEGYGMTETSCVISGMDEGDNLSGHVGSPNPACEVKlvdVPEMNyt 450
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 ---------EILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKI 475
Cdd:PLN02736 451 sedqpyprgEICVRGPIIFKGYYKDEVQTREVIDEDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGEYIAPEKIENVY 530
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 476 GKDKFIEQIAVIADA-KKYVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFERRIHELQKE--LPSFEQVKRF 552
Cdd:PLN02736 531 AKCKFVAQCFVYGDSlNSSLVAVVVVDPEVLKAWAASEGIKYEDLKQLCNDPRVRAAVLADMDAVGREaqLRGFEFAKAV 610
                        570       580       590
                 ....*....|....*....|....*....|....*....
gi 544685990 553 TLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMYNE 591
Cdd:PLN02736 611 TLVPEPFTVENGLLTPTFKVKRPQAKAYFAKAISDMYAE 649
ligase_PEP_1 TIGR03098
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ...
23-472 6.58e-35

acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.


Pssm-ID: 211788 [Multi-domain]  Cd Length: 517  Bit Score: 138.38  E-value: 6.58e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   23 TALRFREQAqwqeMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYI 102
Cdd:TIGR03098  17 TALVHHDRT----LTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGVFVPINPLLKAEQVAHI 92
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  103 VNDADIKILfVGDQEQFDQVYQIANNCPQLMKIVAMKANMHLRDLP---NACYWEDFLNVVPneaefEKRLNSKQLSDLF 179
Cdd:TIGR03098  93 LADCNVRLL-VTSSERLDLLHPALPGCHDLRTLIIVGDPAHASEGHpgeEPASWPKLLALGD-----ADPPHPVIDSDMA 166
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  180 TLIYTSGTTGEPKGVMLDYANLAhqLNAHDLA--LNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATnCYLEDTKHVRDA 257
Cdd:TIGR03098 167 AILYTSGSTGRPKGVVLSHRNLV--AGAQSVAtyLENRPDDRLLAVLPLSFDYGFNQLTTAFYVGAT-VVLHDYLLPRDV 243
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  258 LTTLKP---TVMCAVPrfyekiytavwdkvekaPLhrralfnWAirvgeqhyqieqpsQWLRLQYALADKLVLTKLrALL 334
Cdd:TIGR03098 244 LKALEKhgiTGLAAVP-----------------PL-------WA--------------QLAQLDWPESAAPSLRYL-TNS 284
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  335 GGRikmMPcggAKLEASIGSFFHSigINIKLGYGMTEttATVSCWQDKGF---NPNSIGTLMPNAEVKI----------G 401
Cdd:TIGR03098 285 GGA---MP---RATLSRLRSFLPN--ARLFLMYGLTE--AFRSTYLPPEEvdrRPDSIGKAIPNAEVLVlredgsecapG 354
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  402 EENEILVRGGMVMRGYYKKPEETAKAFT-----EDGFLRT------GDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQY 470
Cdd:TIGR03098 355 EEGELVHRGALVAMGYWNDPEKTAERFRplppfPGELHLPelavwsGDTVRRDEEGFLYFVGRRDEMIKTS-GYRVSPTE 433

                  ..
gi 544685990  471 IE 472
Cdd:TIGR03098 434 VE 435
 
Name Accession Description Interval E-value
FAA1 COG1022
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
7-592 0e+00

Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];


Pssm-ID: 440645 [Multi-domain]  Cd Length: 603  Bit Score: 830.51  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   7 HFVNRFRLQAKKWLNRTALRFREQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAV 86
Cdd:COG1022   12 TLPDLLRRRAARFPDRVALREKEDGIWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAV 91
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  87 AVPIYATNTAKQVEYIVNDADIKILFVGDQEQFDQVYQIANNCPQLMKIVAMKAnMHLRDLPNACYWEDFLNV---VPNE 163
Cdd:COG1022   92 TVPIYPTSSAEEVAYILNDSGAKVLFVEDQEQLDKLLEVRDELPSLRHIVVLDP-RGLRDDPRLLSLDELLALgreVADP 170
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 164 AEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGA 243
Cdd:COG1022  171 AELEARRAAVKPDDLATIIYTSGTTGRPKGVMLTHRNLLSNARALLERLPLGPGDRTLSFLPLAHVFERTVSYYALAAGA 250
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 244 TNCYLEDTKHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVGEQH----YQIEQPSQWLRLQY 319
Cdd:COG1022  251 TVAFAESPDTLAEDLREVKPTFMLAVPRVWEKVYAGIQAKAEEAGGLKRKLFRWALAVGRRYararLAGKSPSLLLRLKH 330
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 320 ALADKLVLTKLRALLGGRIKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVK 399
Cdd:COG1022  331 ALADKLVFSKLREALGGRLRFAVSGGAALGPELARFFRALGIPVLEGYGLTETSPVITVNRPGDNRIGTVGPPLPGVEVK 410
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 400 IGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDK 479
Cdd:COG1022  411 IAEDGEILVRGPNVMKGYYKNPEATAEAFDADGWLHTGDIGELDEDGFLRITGRKKDLIVTSGGKNVAPQPIENALKASP 490
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 480 FIEQIAVIADAKKYVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFERRIHELQKELPSFEQVKRFTLLPQAF 559
Cdd:COG1022  491 LIEQAVVVGDGRPFLAALIVPDFEALGEWAEENGLPYTSYAELAQDPEVRALIQEEVDRANAGLSRAEQIKRFRLLPKEF 570
                        570       580       590
                 ....*....|....*....|....*....|...
gi 544685990 560 STAMEEITPTLKLRRKVIMQRYREQIEEMYNER 592
Cdd:COG1022  571 TIENGELTPTLKLKRKVILEKYADLIEALYAGA 603
VL_LC_FACS_like cd05907
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ...
32-577 0e+00

Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341233 [Multi-domain]  Cd Length: 452  Bit Score: 583.02  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  32 QWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKIL 111
Cdd:cd05907    2 VWQPITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKAL 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 112 FVGDqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqLSDLFTLIYTSGTTGEP 191
Cdd:cd05907   82 FVED-----------------------------------------------------------PDDLATIIYTSGTTGRP 102
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 192 KGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFE-RAWIAYVFHRGATNCYLEDTKHVRDALTTLKPTVMCAVP 270
Cdd:cd05907  103 KGVMLSHRNILSNALALAERLPATEGDRHLSFLPLAHVFErRAGLYVPLLAGARIYFASSAETLLDDLSEVRPTVFLAVP 182
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 RFYEKIYTAVwdKVEKAPLHRRALFNWAIrvgeqhyqieqpsqwlrlqyaladklvltklrallGGRIKMMPCGGAKLEA 350
Cdd:cd05907  183 RVWEKVYAAI--KVKAVPGLKRKLFDLAV-----------------------------------GGRLRFAASGGAPLPA 225
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 351 SIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTE 430
Cdd:cd05907  226 ELLHFFRALGIPVYEGYGLTETSAVVTLNPPGDNRIGTVGKPLPGVEVRIADDGEILVRGPNVMLGYYKNPEATAEALDA 305
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 431 DGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEEYAK 510
Cdd:cd05907  306 DGWLHTGDLGEIDEDGFLHITGRKKDLIITSGGKNISPEPIENALKASPLISQAVVIGDGRPFLVALIVPDPEALEAWAE 385
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990 511 QLNIKYQDRIELIKHSDIIQMFERRIHELQKELPSFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVI 577
Cdd:cd05907  386 EHGIAYTDVAELAANPAVRAEIEAAVEAANARLSRYEQIKKFLLLPEPFTIENGELTPTLKLKRPVI 452
LC-FACS_euk cd05927
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ...
34-589 3.97e-134

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.


Pssm-ID: 341250 [Multi-domain]  Cd Length: 545  Bit Score: 402.36  E-value: 3.97e-134
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  34 QEMSWQTFQQ---EIDRFSYALIAQHIDI--QDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADI 108
Cdd:cd05927    1 GPYEWISYKEvaeRADNIGSALRSLGGKPapASFVGIYSINRPEWIISELACYAYSLVTVPLYDTLGPEAIEYILNHAEI 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 109 KILFVGDQeqfdqvyqianncpqlMKIVAMKANMHLrdlpnacYWEDFLNVVPNEAEfekrlnskqlsDLFTLIYTSGTT 188
Cdd:cd05927   81 SIVFCDAG----------------VKVYSLEEFEKL-------GKKNKVPPPPPKPE-----------DLATICYTSGTT 126
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 189 GEPKGVMLDYANLAHQLNAHDLALNV----NEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLE-DTKHVRDALTTLKP 263
Cdd:cd05927  127 GNPKGVMLTHGNIVSNVAGVFKILEIlnkiNPTDVYISYLPLAHIFERVVEALFLYHGAKIGFYSgDIRLLLDDIKALKP 206
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 264 TVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVGEQHYQ--IEQPSQWLrlqyalaDKLVLTKLRALLGGRIKMM 341
Cdd:cd05927  207 TVFPGVPRVLNRIYDKIFNKVQAKGPLKRKLFNFALNYKLAELRsgVVRASPFW-------DKLVFNKIKQALGGNVRLM 279
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 342 PCGGAKLEASIGSFFHSI-GINIKLGYGMTETTA--TVSCWQDKgfNPNSIGTLMPNAEVKIGE-------------ENE 405
Cdd:cd05927  280 LTGSAPLSPEVLEFLRVAlGCPVLEGYGQTECTAgaTLTLPGDT--SVGHVGGPLPCAEVKLVDvpemnydakdpnpRGE 357
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 406 ILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIA 485
Cdd:cd05927  358 VCIRGPNVFSGYYKDPEKTAEALDEDGWLHTGDIGEWLPNGTLKIIDRKKNIFKLSQGEYVAPEKIENIYARSPFVAQIF 437
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 486 VIADAKK-YVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFERRIHELQKE--LPSFEQVKRFTLLPQAFSTA 562
Cdd:cd05927  438 VYGDSLKsFLVAIVVPDPDVLKEWAASKGGGTGSFEELCKNPEVKKAILEDLVRLGKEngLKGFEQVKAIHLEPEPFSVE 517
                        570       580
                 ....*....|....*....|....*..
gi 544685990 563 MEEITPTLKLRRKVIMQRYREQIEEMY 589
Cdd:cd05927  518 NGLLTPTFKLKRPQLKKYYKKQIDEMY 544
LC_FACS_bac1 cd17641
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ...
25-581 1.01e-116

bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341296 [Multi-domain]  Cd Length: 569  Bit Score: 358.27  E-value: 1.01e-116
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  25 LRFREQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVN 104
Cdd:cd17641    1 LREKDFGIWQEFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLN 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 105 DADIKILFVGDQEQFDQVYQIANNCPQLMKIVAM-KANMHLRDLPNACYWEDFLNVVPNEA-----EFEKRLNSKQLSDL 178
Cdd:cd17641   81 YTGARVVIAEDEEQVDKLLEIADRIPSVRYVIYCdPRGMRKYDDPRLISFEDVVALGRALDrrdpgLYEREVAAGKGEDV 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 179 FTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWI--AYVFHRGATNCYLEDTKHVRD 256
Cdd:cd17641  161 AVLCTTSGTTGKPKLAMLSHGNFLGHCAAYLAADPLGPGDEYVSVLPLPWIGEQMYSvgQALVCGFIVNFPEEPETMMED 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 257 aLTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVG----EQHYQIEQPSQWLRLQYALADKLVLTKLRA 332
Cdd:cd17641  241 -LREIGPTFVLLPPRVWEGIAADVRARMMDATPFKRFMFELGMKLGlralDRGKRGRPVSLWLRLASWLADALLFRPLRD 319
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 333 LLG-GRIKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKIGEENEILVRGG 411
Cdd:cd17641  320 RLGfSRLRSAATGGAALGPDTFRFFHAIGVPLKQLYGQTELAGAYTVHRDGDVDPDTVGVPFPGTEVRIDEVGEILVRSP 399
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 412 MVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAK 491
Cdd:cd17641  400 GVFVGYYKNPEATAEDFDEDGWLHTGDAGYFKENGHLVVIDRAKDVGTTSDGTRFSPQFIENKLKFSPYIAEAVVLGAGR 479
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 492 KYVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFERRIHELQKELPSFEQVKRFTLLPQAFSTAMEEITPTLK 571
Cdd:cd17641  480 PYLTAFICIDYAIVGKWAEQRGIAFTTYTDLASRPEVYELIRKEVEKVNASLPEAQRIRRFLLLYKELDADDGELTRTRK 559
                        570
                 ....*....|
gi 544685990 572 LRRKVIMQRY 581
Cdd:cd17641  560 VRRGVIAEKY 569
LC_FACS_like cd17640
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ...
37-577 2.78e-108

Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341295 [Multi-domain]  Cd Length: 468  Bit Score: 333.17  E-value: 2.78e-108
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  37 SWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQ 116
Cdd:cd17640    7 TYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVALVVEND 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 117 EqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqlSDLFTLIYTSGTTGEPKGVML 196
Cdd:cd17640   87 S----------------------------------------------------------DDLATIIYTSGTTGNPKGVML 108
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 197 DYANLAHQLnaHDLALNVNED--DVSLSFLPLSHIFERAWIAYVFHRGATNCYlEDTKHVRDALTTLKPTVMCAVPRFYE 274
Cdd:cd17640  109 THANLLHQI--RSLSDIVPPQpgDRFLSILPIWHSYERSAEYFIFACGCSQAY-TSIRTLKDDLKRVKPHYIVSVPRLWE 185
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 275 KIYTAVWDKVEKAPLHRRALFnwairvgeqhyqieqpsqwlrlqyaladklvltkLRALLGGRIKMMPCGGAKLEASIGS 354
Cdd:cd17640  186 SLYSGIQKQVSKSSPIKQFLF----------------------------------LFFLSGGIFKFGISGGGALPPHVDT 231
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 355 FFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEE 423
Cdd:cd17640  232 FFEAIGIEVLNGYGLTETSPVVSARRLKCNVRGSVGRPLPGTEIKIvdpegnvvlppGEKGIVWVRGPQVMKGYYKNPEA 311
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 424 TAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFD 503
Cdd:cd17640  312 TSKVLDSDGWFNTGDLGWLTCGGELVLTGRAKDTIVLSNGENVEPQPIEEALMRSPFIEQIMVVGQDQKRLGALIVPNFE 391
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 504 SLEEYAKQLNIKY-QDRIELIKHSDIIQMFERRIHELQKELP---SFEQVKRFTLLPQAFsTAMEEITPTLKLRRKVI 577
Cdd:cd17640  392 ELEKWAKESGVKLaNDRSQLLASKKVLKLYKNEIKDEISNRPgfkSFEQIAPFALLEEPF-IENGEMTQTMKIKRNVV 468
ACSBG_like cd05933
Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very ...
28-589 1.14e-100

Bubblegum-like very long-chain fatty acid CoA synthetase (VL-FACS); This family of very long-chain fatty acid CoA synthetase is named bubblegum because Drosophila melanogaster mutant bubblegum (BGM) has elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene of this family. The human homolog (hsBG) has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. VL-FACS is involved in the first reaction step of very long chain fatty acid degradation. It catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341256 [Multi-domain]  Cd Length: 596  Bit Score: 317.76  E-value: 1.14e-100
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  28 REQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDAD 107
Cdd:cd05933    1 KRGDKWHTLTYKEYYEACRQAAKAFLKLGLERFHGVGILGFNSPEWFIAAVGAIFAGGIAVGIYTTNSPEACQYVAETSE 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 108 IKILFVGDQEQFDQVYQIANNCPQLMKIVAMKANMHLRdLPNACYWEDF--LNVVPNEAEFEKRLNSKQLSDLFTLIYTS 185
Cdd:cd05933   81 ANILVVENQKQLQKILQIQDKLPHLKAIIQYKEPLKEK-EPNLYSWDEFmeLGRSIPDEQLDAIISSQKPNQCCTLIYTS 159
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 186 GTTGEPKGVMLDYANL---AHQLNAH-DLALNVNEDDVSLSFLPLSHIFER---AWIAyVFHRGATncYLEDtkhvRDAL 258
Cdd:cd05933  160 GTTGMPKGVMLSHDNItwtAKAASQHmDLRPATVGQESVVSYLPLSHIAAQildIWLP-IKVGGQV--YFAQ----PDAL 232
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 259 -----TTLK---PTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVGEQHYQIEQPSQW-LRLQYALADKLVLTK 329
Cdd:cd05933  233 kgtlvKTLRevrPTAFMGVPRVWEKIQEKMKAVGAKSGTLKRKIASWAKGVGLETNLKLMGGESpSPLFYRLAKKLVFKK 312
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 330 LRALLG-GRIKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTA--TVScwQDKGFNPNSIGTLMPNAEVKIGEEN-- 404
Cdd:cd05933  313 VRKALGlDRCQKFFTGAAPISRETLEFFLSLNIPIMELYGMSETSGphTIS--NPQAYRLLSCGKALPGCKTKIHNPDad 390
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 ---EILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKD-KF 480
Cdd:cd05933  391 gigEICFWGRHVFMGYLNMEDKTEEAIDEDGWLHSGDLGKLDEDGFLYITGRIKELIITAGGENVPPVPIEDAVKKElPI 470
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 481 IEQIAVIADAKKYVSALI-VPC---------FDSLE----EYAKQLNIKYQDRIELIKHSD--IIQMFERRIHELQKELP 544
Cdd:cd05933  471 ISNAMLIGDKRKFLSMLLtLKCevnpetgepLDELTeeaiEFCRKLGSQATRVSEIAGGKDpkVYEAIEEGIKRVNKKAI 550
                        570       580       590       600
                 ....*....|....*....|....*....|....*....|....*.
gi 544685990 545 SFEQ-VKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMY 589
Cdd:cd05933  551 SNAQkIQKWVILEKDFSVPGGELGPTMKLKRPVVAKKYKDEIDKLY 596
AMP-binding pfam00501
AMP-binding enzyme;
12-461 1.01e-97

AMP-binding enzyme;


Pssm-ID: 459834 [Multi-domain]  Cd Length: 417  Bit Score: 304.24  E-value: 1.01e-97
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   12 FRLQAKKWLNRTALRFREqaqWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:pfam00501   1 LERQAARTPDKTALEVGE---GRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLN 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   92 ATNTAKQVEYIVNDADIKILFVGDQEQFDQVYQIANNCPQLMKIVAMKANMHLRDlpnacywEDFLNVVPNEAEFEKRLN 171
Cdd:pfam00501  78 PRLPAEELAYILEDSGAKVLITDDALKLEELLEALGKLEVVKLVLVLDRDPVLKE-------EPLPEEAKPADVPPPPPP 150
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  172 SKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAH----DLALNVNEDDVSLSFLPLSHIFERAWIAY-VFHRGATNC 246
Cdd:pfam00501 151 PPDPDDLAYIIYTSGTTGKPKGVMLTHRNLVANVLSIkrvrPRGFGLGPDDRVLSTLPLFHDFGLSLGLLgPLLAGATVV 230
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  247 YLE-----DTKHVRDALTTLKPTVMCAVPRFYEKIytavwdkVEKAPLHRRALfnwairvgeqhyqieqpsqwlrlqyal 321
Cdd:pfam00501 231 LPPgfpalDPAALLELIERYKVTVLYGVPTLLNML-------LEAGAPKRALL--------------------------- 276
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  322 adklvltklrallgGRIKMMPCGGAKLEASIGSFFHSIGIN-IKLGYGMTETTATVSC---WQDKGFNPNSIGTLMPNAE 397
Cdd:pfam00501 277 --------------SSLRLVLSGGAPLPPELARRFRELFGGaLVNGYGLTETTGVVTTplpLDEDLRSLGSVGRPLPGTE 342
                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990  398 VKI-----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTS 461
Cdd:pfam00501 343 VKIvddetgepvppGEPGELCVRGPGVMKGYLNDPELTAEAFDEDGWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
LC_FACS_bac cd05932
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ...
32-581 9.04e-96

Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341255 [Multi-domain]  Cd Length: 508  Bit Score: 302.08  E-value: 9.04e-96
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  32 QWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKIL 111
Cdd:cd05932    3 QVVEFTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKAL 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 112 FVGdqeQFDQVYQIANNCPQLMKIVAMkanmHLRDLPNACY-WEDFLNVVPNEAEFEKRLNSKqlsdLFTLIYTSGTTGE 190
Cdd:cd05932   83 FVG---KLDDWKAMAPGVPEGLISISL----PPPSAANCQYqWDDLIAQHPPLEERPTRFPEQ----LATLIYTSGTTGQ 151
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 191 PKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLEDT--KHVRDaLTTLKPTVMCA 268
Cdd:cd05932  152 PKGVMLTFGSFAWAAQAGIEHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLVAFAESldTFVED-VQRARPTLFFS 230
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 269 VPRFYEKIYTAVWDKVEKAPLHRRalfnwairvgeqhyqieqpsqwlrLQYALADKLVLTKLRALLG-GRIKMMPCGGAK 347
Cdd:cd05932  231 VPRLWTKFQQGVQDKIPQQKLNLL------------------------LKIPVVNSLVKRKVLKGLGlDQCRLAGCGSAP 286
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 348 LEASIGSFFHSIGINIKLGYGMTETTAtVSCWQDKGFNP-NSIGTLMPNAEVKIGEENEILVRGGMVMRGYYKKPEETAK 426
Cdd:cd05932  287 VPPALLEWYRSLGLNILEAYGMTENFA-YSHLNYPGRDKiGTVGNAGPGVEVRISEDGEILVRSPALMMGYYKDPEATAE 365
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 427 AFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPcfdSLE 506
Cdd:cd05932  366 AFTADGFLRTGDKGELDADGNLTITGRVKDIFKTSKGKYVAPAPIENKLAEHDRVEMVCVIGSGLPAPLALVVL---SEE 442
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 507 EYAKQLNikyQDRIELIKHsdiiqmFERRIHELQKELPSFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRY 581
Cdd:cd05932  443 ARLRADA---FARAELEAS------LRAHLARVNSTLDSHEQLAGIVVVKDPWSIDNGILTPTLKIKRNVLEKAY 508
PLN02736 PLN02736
long-chain acyl-CoA synthetase
26-591 6.92e-93

long-chain acyl-CoA synthetase


Pssm-ID: 178337 [Multi-domain]  Cd Length: 651  Bit Score: 298.94  E-value: 6.92e-93
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  26 RFREQAQWQEMSWQTFQQEIDR---FSYALIAQHIDIQDKIGIFANNMPRWTIADFgAMQARA-VAVPIYATNTAKQVEY 101
Cdd:PLN02736  66 RIRVDGTVGEYKWMTYGEAGTArtaIGSGLVQHGIPKGACVGLYFINRPEWLIVDH-ACSAYSyVSVPLYDTLGPDAVKF 144
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 102 IVNDADIKILFVGDQeQFDQVYQIANNCPQLMKIVAMK-ANMHLRDLPNACYWEdflnVVP-NEAEFEKRLNSKQL---- 175
Cdd:PLN02736 145 IVNHAEVAAIFCVPQ-TLNTLLSCLSEIPSVRLIVVVGgADEPLPSLPSGTGVE----IVTySKLLAQGRSSPQPFrppk 219
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 176 -SDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERA-WIAYVFHRGATNCYLEDTKH 253
Cdd:PLN02736 220 pEDVATICYTSGTTGTPKGVVLTHGNLIANVAGSSLSTKFYPSDVHISYLPLAHIYERVnQIVMLHYGVAVGFYQGDNLK 299
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 254 VRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVGEQhyQIEQ---PSqwlrlqyALADKLVLTKL 330
Cdd:PLN02736 300 LMDDLAALRPTIFCSVPRLYNRIYDGITNAVKESGGLKERLFNAAYNAKKQ--ALENgknPS-------PMWDRLVFNKI 370
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 331 RALLGGRIKMMPCGGAKLEASIGSFFH-SIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVK---IGEEN-- 404
Cdd:PLN02736 371 KAKLGGRVRFMSSGASPLSPDVMEFLRiCFGGRVLEGYGMTETSCVISGMDEGDNLSGHVGSPNPACEVKlvdVPEMNyt 450
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 ---------EILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKI 475
Cdd:PLN02736 451 sedqpyprgEICVRGPIIFKGYYKDEVQTREVIDEDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGEYIAPEKIENVY 530
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 476 GKDKFIEQIAVIADA-KKYVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFERRIHELQKE--LPSFEQVKRF 552
Cdd:PLN02736 531 AKCKFVAQCFVYGDSlNSSLVAVVVVDPEVLKAWAASEGIKYEDLKQLCNDPRVRAAVLADMDAVGREaqLRGFEFAKAV 610
                        570       580       590
                 ....*....|....*....|....*....|....*....
gi 544685990 553 TLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMYNE 591
Cdd:PLN02736 611 TLVPEPFTVENGLLTPTFKVKRPQAKAYFAKAISDMYAE 649
LC_FACS_euk1 cd17639
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ...
162-577 2.94e-89

Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.


Pssm-ID: 341294 [Multi-domain]  Cd Length: 507  Bit Score: 285.26  E-value: 2.94e-89
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 162 NEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNA--HDLALNVNEDDVSLSFLPLSHIFERAWIAYVF 239
Cdd:cd17639   74 NETECSAIFTDGKPDDLACIMYTSGSTGNPKGVMLTHGNLVAGIAGlgDRVPELLGPDDRYLAYLPLAHIFELAAENVCL 153
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 240 HRGATNCY-----LED--TKHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAirvgeqhYQIEqpS 312
Cdd:cd17639  154 YRGGTIGYgsprtLTDksKRGCKGDLTEFKPTLMVGVPAIWDTIRKGVLAKLNPMGGLKRTLFWTA-------YQSK--L 224
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 313 QWLRLQY--ALADKLVLTKLRALLGGRIKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTA--TVSCWQDkgFNPNS 388
Cdd:cd17639  225 KALKEGPgtPLLDELVFKKVRAALGGRLRYMLSGGAPLSADTQEFLNIVLCPVIQGYGLTETCAggTVQDPGD--LETGR 302
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 389 IGTLMPNAEVKIGE-------------ENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLK 455
Cdd:cd17639  303 VGPPLPCCEIKLVDweeggystdkpppRGEILIRGPNVFKGYYKNPEKTKEAFDGDGWFHTGDIGEFHPDGTLKIIDRKK 382
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 456 ELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIAD-AKKYVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFER 534
Cdd:cd17639  383 DLVKLQNGEYIALEKLESIYRSNPLVNNICVYADpDKSYPVAIVVPNEKHLTKLAEKHGVINSEWEELCEDKKLQKAVLK 462
                        410       420       430       440
                 ....*....|....*....|....*....|....*....|....*
gi 544685990 535 RIHELQKE--LPSFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVI 577
Cdd:cd17639  463 SLAETARAagLEKFEIPQGVVLLDEEWTPENGLVTAAQKLKRKEI 507
LC_FACL_like cd05914
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ...
35-574 1.35e-87

Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.


Pssm-ID: 341240 [Multi-domain]  Cd Length: 463  Bit Score: 279.33  E-value: 1.35e-87
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  35 EMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVG 114
Cdd:cd05914    7 PLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKAIFVS 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 115 DQEqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqlsDLFTLIYTSGTTGEPKGV 194
Cdd:cd05914   87 DED-----------------------------------------------------------DVALINYTSGTTGNSKGV 107
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 195 MLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAW-IAYVFHRGATNCYLEDTKHVR-DALTTLKPTVMCAVPRF 272
Cdd:cd05914  108 MLTYRNIVSNVDGVKEVVLLGKGDKILSILPLHHIYPLTFtLLLPLLNGAHVVFLDKIPSAKiIALAFAQVTPTLGVPVP 187
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 273 Y--EKIYtavwdKVEKAPLHRRALFNWAIRVGEQHYQIEqpsqwlrlqyaladKLVLTKLRALLGGRIKMMPCGGAKLEA 350
Cdd:cd05914  188 LviEKIF-----KMDIIPKLTLKKFKFKLAKKINNRKIR--------------KLAFKKVHEAFGGNIKEFVIGGAKINP 248
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 351 SIGSFFHSIGINIKLGYGMTETTATVScwqdkgFNP------NSIGTLMPNAEVKI------GEENEILVRGGMVMRGYY 418
Cdd:cd05914  249 DVEEFLRTIGFPYTIGYGMTETAPIIS------YSPpnrirlGSAGKVIDGVEVRIdspdpaTGEGEIIVRGPNVMKGYY 322
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 419 KKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVsALI 498
Cdd:cd05914  323 KNPEATAEAFDKDGWFHTGDLGKIDAEGYLYIRGRKKEMIVLSSGKNIYPEEIEAKINNMPFVLESLVVVQEKKLV-ALA 401
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 499 VPCFDSLEEYAKQLnikyQDRIELIKhsdiiqmfERRIHELQKELPSFEQVKRFTLLPQAFstameEITPTLKLRR 574
Cdd:cd05914  402 YIDPDFLDVKALKQ----RNIIDAIK--------WEVRDKVNQKVPNYKKISKVKIVKEEF-----EKTPKGKIKR 460
PLN02430 PLN02430
long-chain-fatty-acid-CoA ligase
12-589 9.37e-83

long-chain-fatty-acid-CoA ligase


Pssm-ID: 178049 [Multi-domain]  Cd Length: 660  Bit Score: 272.46  E-value: 9.37e-83
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  12 FRLQAKKWLNRTALRFRE--QAQWQEMSWQTFQQ---EIDRFSYALIAQHIDIQDKIGIFANNMPRWTIAdfgaMQARA- 85
Cdd:PLN02430  48 FSKSVEKYPDNKMLGWRRivDGKVGPYMWKTYKEvyeEVLQIGSALRASGAEPGSRVGIYGSNCPQWIVA----MEACAa 123
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  86 ---VAVPIYATNTAKQVEYIVNDADIKILFVGDQeqfdQVYQIAN-NCPQLMKIVAMKANMHLRDLP---------NACY 152
Cdd:PLN02430 124 hslICVPLYDTLGPGAVDYIVDHAEIDFVFVQDK----KIKELLEpDCKSAKRLKAIVSFTSVTEEEsdkasqigvKTYS 199
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 153 WEDFLNVvpnEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNED-----DVSLSFLPLS 227
Cdd:PLN02430 200 WIDFLHM---GKENPSETNPPKPLDICTIMYTSGTSGDPKGVVLTHEAVATFVRGVDLFMEQFEDkmthdDVYLSFLPLA 276
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 228 HIFERAWIAYVFHRGATNCYLE-DTKHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRvgeqhY 306
Cdd:PLN02430 277 HILDRMIEEYFFRKGASVGYYHgDLNALRDDLMELKPTLLAGVPRVFERIHEGIQKALQELNPRRRLIFNALYK-----Y 351
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 307 QIeqpsQWLRLQYA------LADKLVLTKLRALLGGRIKMMPCGGAKLEASIGSFFH--SIGINIKlGYGMTETTATVS- 377
Cdd:PLN02430 352 KL----AWMNRGYShkkaspMADFLAFRKVKAKLGGRLRLLISGGAPLSTEIEEFLRvtSCAFVVQ-GYGLTETLGPTTl 426
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 378 CWQDKGFNPNSIGTLMPNAEVKIGE-------------ENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDS 444
Cdd:PLN02430 427 GFPDEMCMLGTVGAPAVYNELRLEEvpemgydplgeppRGEICVRGKCLFSGYYKNPELTEEVM-KDGWFHTGDIGEILP 505
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 445 CGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADA-KKYVSALIVPCFDSLEEYAKQLNIKyqDRIELI 523
Cdd:PLN02430 506 NGVLKIIDRKKNLIKLSQGEYVALEYLENVYGQNPIVEDIWVYGDSfKSMLVAVVVPNEENTNKWAKDNGFT--GSFEEL 583
                        570       580       590       600       610       620       630
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 544685990 524 KHSDiiQMFERRIHELQ-----KELPSFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMY 589
Cdd:PLN02430 584 CSLP--ELKEHILSELKstaekNKLRGFEYIKGVILETKPFDVERDLVTATLKKRRNNLLKYYQVEIDEMY 652
MenE/FadK COG0318
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ...
12-587 4.14e-78

O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis


Pssm-ID: 440087 [Multi-domain]  Cd Length: 452  Bit Score: 254.35  E-value: 4.14e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  12 FRLQAKKWLNRTALRFreqaQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:COG0318    5 LRRAAARHPDRPALVF----GGRRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLN 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  92 ATNTAKQVEYIVNDADIKILFVgdqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrln 171
Cdd:COG0318   81 PRLTAEELAYILEDSGARALVT---------------------------------------------------------- 102
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 172 skqlsdlFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFerAWIAYVF---HRGATNCYL 248
Cdd:COG0318  103 -------ALILYTSGTTGRPKGVMLTHRNLLANAAAIAAALGLTPGDVVLVALPLFHVF--GLTVGLLaplLAGATLVLL 173
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 249 E--DTKHVRDALTTLKPTVMCAVPRFYEKIytavwdkvekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADKLV 326
Cdd:COG0318  174 PrfDPERVLELIERERVTVLFGVPTMLARL----------------------------------------LRHPEFARYD 213
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 327 LTKLRALLggrikmmpCGGAKL-EASIGSFFHSIGINIKLGYGMTETTATVSC-WQDKGFN-PNSIGTLMPNAEVKI--- 400
Cdd:COG0318  214 LSSLRLVV--------SGGAPLpPELLERFEERFGVRIVEGYGLTETSPVVTVnPEDPGERrPGSVGRPLPGVEVRIvde 285
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNGKYIAPQYIEG 473
Cdd:COG0318  286 dgrelppGEVGEIVVRGPNVMKGYWNDPEATAEAF-RDGWLRTGDLGRLDEDGYLYIVGRKKDMII-SGGENVYPAEVEE 363
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 474 KIGKDKFIEQIAVIA--DAKKY--VSALIVPCFDSLEeyakqlnikyqDRIELIKHsdiiqmferriheLQKELPSFEQV 549
Cdd:COG0318  364 VLAAHPGVAEAAVVGvpDEKWGerVVAFVVLRPGAEL-----------DAEELRAF-------------LRERLARYKVP 419
                        570       580       590       600
                 ....*....|....*....|....*....|....*....|
gi 544685990 550 KRFTLLpqafstamEEI--TPTLKLRRKVIMQRYREQIEE 587
Cdd:COG0318  420 RRVEFV--------DELprTASGKIDRRALRERYAAGALE 451
PLN02861 PLN02861
long-chain-fatty-acid-CoA ligase
38-591 2.50e-76

long-chain-fatty-acid-CoA ligase


Pssm-ID: 178452 [Multi-domain]  Cd Length: 660  Bit Score: 255.54  E-value: 2.50e-76
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  38 WQTFQQEID---RFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVg 114
Cdd:PLN02861  77 WLTYKEVYDaaiRIGSAIRSRGVNPGDRCGIYGSNCPEWIIAMEACNSQGITYVPLYDTLGANAVEFIINHAEVSIAFV- 155
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 115 DQEQFDQVYQIANNCPQLMKI------VAMKANMHLRDLPNACY-WEDFLNVVPNEAEfekrLNSKQLSDLFTLIYTSGT 187
Cdd:PLN02861 156 QESKISSILSCLPKCSSNLKTivsfgdVSSEQKEEAEELGVSCFsWEEFSLMGSLDCE----LPPKQKTDICTIMYTSGT 231
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 188 TGEPKGVMLDYANLAHQLNAHDLALNVN-----EDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLE-DTKHVRDALTTL 261
Cdd:PLN02861 232 TGEPKGVILTNRAIIAEVLSTDHLLKVTdrvatEEDSYFSYLPLAHVYDQVIETYCISKGASIGFWQgDIRYLMEDVQAL 311
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 262 KPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIrvgeqHYQIEQPSQWLRLQYA--LADKLVLTKLRALLGGRIK 339
Cdd:PLN02861 312 KPTIFCGVPRVYDRIYTGIMQKISSGGMLRKKLFDFAY-----NYKLGNLRKGLKQEEAspRLDRLVFDKIKEGLGGRVR 386
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 340 MMPCGGAKLEASIGSFFHSIGI-NIKLGYGMTETTAtvSCWQDKGFNPNSIGTL---MPNAEVKIGE------------- 402
Cdd:PLN02861 387 LLLSGAAPLPRHVEEFLRVTSCsVLSQGYGLTESCG--GCFTSIANVFSMVGTVgvpMTTIEARLESvpemgydalsdvp 464
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 403 ENEILVRGGMVMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIE 482
Cdd:PLN02861 465 RGEICLRGNTLFSGYHKRQDLTEEVLI-DGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSQGEYVAVENLENTYSRCPLIA 543
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 483 QIAVIADA-KKYVSALIVPCFDSLEEYAKQLNiKYQDRIELIKHSDIIQMFERRIHELQKE--LPSFEQVKRFTLLPQAF 559
Cdd:PLN02861 544 SIWVYGNSfESFLVAVVVPDRQALEDWAANNN-KTGDFKSLCKNLKARKYILDELNSTGKKlqLRGFEMLKAIHLEPNPF 622
                        570       580       590
                 ....*....|....*....|....*....|..
gi 544685990 560 STAMEEITPTLKLRRKVIMQRYREQIEEMYNE 591
Cdd:PLN02861 623 DIERDLITPTFKLKRPQLLKYYKDCIDQLYSE 654
PLN02614 PLN02614
long-chain acyl-CoA synthetase
12-589 6.52e-73

long-chain acyl-CoA synthetase


Pssm-ID: 166255 [Multi-domain]  Cd Length: 666  Bit Score: 246.47  E-value: 6.52e-73
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  12 FRLQAKKWLNRTALRFRE--QAQWQEMSWQTFQQEID---RFSYALIAQHIDIQDKIGIFANNMPRWTIAdFGAMQARAV 86
Cdd:PLN02614  51 FRMSVEKYPNNPMLGRREivDGKPGKYVWQTYQEVYDiviKLGNSLRSVGVKDEAKCGIYGANSPEWIIS-MEACNAHGL 129
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  87 -AVPIYATNTAKQVEYIVNDADIKILFVgDQEQFDQVYQIANNCPQLMKIVAMKANMHLRDLPNA-------CYWEDFLN 158
Cdd:PLN02614 130 yCVPLYDTLGAGAVEFIISHSEVSIVFV-EEKKISELFKTCPNSTEYMKTVVSFGGVSREQKEEAetfglviYAWDEFLK 208
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 159 VVPNEaefEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLD-------YANLAHQLNAHDLALNvnEDDVSLSFLPLSHIFE 231
Cdd:PLN02614 209 LGEGK---QYDLPIKKKSDICTIMYTSGTTGDPKGVMISnesivtlIAGVIRLLKSANAALT--VKDVYLSYLPLAHIFD 283
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 232 RAWI-AYVFHRGATNCYLEDTKHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWA-------IRVGE 303
Cdd:PLN02614 284 RVIEeCFIQHGAAIGFWRGDVKLLIEDLGELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFVFDSAfsykfgnMKKGQ 363
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 304 QHYQIEqpsqwlrlqyALADKLVLTKLRALLGGRIKMMPCGGAKLEASIGSFFHSIGI-NIKLGYGMTETTA-TVSCWQD 381
Cdd:PLN02614 364 SHVEAS----------PLCDKLVFNKVKQGLGGNVRIILSGAAPLASHVESFLRVVACcHVLQGYGLTESCAgTFVSLPD 433
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 382 KGFNPNSIGTLMPNAEVKIGE-------------ENEILVRGGMVMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNL 448
Cdd:PLN02614 434 ELDMLGTVGPPVPNVDIRLESvpemeydalastpRGEICIRGKTLFSGYYKREDLTKEVLI-DGWLHTGDVGEWQPNGSM 512
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 449 FITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADA-KKYVSALIVPCFDSLEEYAKQ--LNIKYQDRIELIKH 525
Cdd:PLN02614 513 KIIDRKKNIFKLSQGEYVAVENIENIYGEVQAVDSVWVYGNSfESFLVAIANPNQQILERWAAEngVSGDYNALCQNEKA 592
                        570       580       590       600       610       620
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 544685990 526 SDIIqMFERRIHELQKELPSFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMY 589
Cdd:PLN02614 593 KEFI-LGELVKMAKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMY 655
PRK06187 PRK06187
long-chain-fatty-acid--CoA ligase; Validated
12-511 1.80e-66

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235730 [Multi-domain]  Cd Length: 521  Bit Score: 225.45  E-value: 1.80e-66
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  12 FRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIy 91
Cdd:PRK06187  12 LRHGARKHPDKEAVYFDGR----RTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAVLHPI- 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  92 atNT---AKQVEYIVNDADIKILFVgDQEQFDQVYQIANNCPQLMKIVAMKANMHLRDLPNACYWEDFLNVVPNEAEF-E 167
Cdd:PRK06187  87 --NIrlkPEEIAYILNDAEDRVVLV-DSEFVPLLAAILPQLPTVRTVIVEGDGPAAPLAPEVGEYEELLAAASDTFDFpD 163
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 168 KRLNskqlsDLFTLIYTSGTTGEPKGVMLDYANL-AHQLNAHDlALNVNEDDVSLSFLPLSHIFerAW-IAYV-FHRGAT 244
Cdd:PRK06187 164 IDEN-----DAAAMLYTSGTTGHPKGVVLSHRNLfLHSLAVCA-WLKLSRDDVYLVIVPMFHVH--AWgLPYLaLMAGAK 235
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 245 NCYLE--DTKHVRDALTTLKPTVMCAVPrfyekiytAVWDKVEKAPLHRRALFNwairvgeqhyqieqpsqwlrlqyala 322
Cdd:PRK06187 236 QVIPRrfDPENLLDLIETERVTFFFAVP--------TIWQMLLKAPRAYFVDFS-------------------------- 281
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 323 dklvltklrallggRIKMMPCGGAKL-EASIGSFFHSIGINIKLGYGMTETTATVSC----WQDKGFNP--NSIGTLMPN 395
Cdd:PRK06187 282 --------------SLRLVIYGGAALpPALLREFKEKFGIDLVQGYGMTETSPVVSVlppeDQLPGQWTkrRSAGRPLPG 347
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 396 AEVKI------------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNG 463
Cdd:PRK06187 348 VEARIvdddgdelppdgGEVGEIIVRGPWLMQGYWNRPEATAETI-DGGWLHTGDVGYIDEDGYLYITDRIKDVII-SGG 425
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 464 KYIAPQYIEGKIGKDKFIEQIAVIA--DAK--KYVSALIVPC------FDSLEEYAKQ 511
Cdd:PRK06187 426 ENIYPRELEDALYGHPAVAEVAVIGvpDEKwgERPVAVVVLKpgatldAKELRAFLRG 483
PLN02387 PLN02387
long-chain-fatty-acid-CoA ligase family protein
35-589 9.58e-64

long-chain-fatty-acid-CoA ligase family protein


Pssm-ID: 215217 [Multi-domain]  Cd Length: 696  Bit Score: 222.30  E-value: 9.58e-64
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  35 EMSWQTFQQEIDR---FSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKIL 111
Cdd:PLN02387 103 EYEWITYGQVFERvcnFASGLVALGHNKEERVAIFADTRAEWLIALQGCFRQNITVVTIYASLGEEALCHSLNETEVTTV 182
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 112 fVGDQEQFDQVYQIANNCPQLMKIVAMKANMHLRD--LPNACYW--EDFLNV--VPNEAEFEKRLNSKqlSDLFTLIYTS 185
Cdd:PLN02387 183 -ICDSKQLKKLIDISSQLETVKRVIYMDDEGVDSDssLSGSSNWtvSSFSEVekLGKENPVDPDLPSP--NDIAVIMYTS 259
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 186 GTTGEPKGVMLDYANLAHQLNAHDLAL-NVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCY-----LEDT-----KHV 254
Cdd:PLN02387 260 GSTGLPKGVMMTHGNIVATVAGVMTVVpKLGKNDVYLAYLPLAHILELAAESVMAAVGAAIGYgspltLTDTsnkikKGT 339
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 255 RDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAI--RVGeqhyQIEqpSQWL---RLQYALADKLVLTK 329
Cdd:PLN02387 340 KGDASALKPTLMTAVPAILDRVRDGVRKKVDAKGGLAKKLFDIAYkrRLA----AIE--GSWFgawGLEKLLWDALVFKK 413
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 330 LRALLGGRIKMMPCGGAKLEASIGSFFH-SIGINIKLGYGMTETTA--TVSCWQDKgfNPNSIGTLMPNAEVKI--GEE- 403
Cdd:PLN02387 414 IRAVLGGRIRFMLSGGAPLSGDTQRFINiCLGAPIGQGYGLTETCAgaTFSEWDDT--SVGRVGPPLPCCYVKLvsWEEg 491
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 404 -----------NEILVRGGMVMRGYYKKPEETAKAFTEDG----FLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAP 468
Cdd:PLN02387 492 gylisdkpmprGEIVIGGPSVTLGYFKNQEKTDEVYKVDErgmrWFYTGDIGQFHPDGCLEIIDRKKDIVKLQHGEYVSL 571
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 469 QYIEGKIGKDKFIEQIAVIADA-KKYVSALIVPCFDSLEEYAKQLNIKYQDRIELIKHSDIIQMFERRIHELQKE--LPS 545
Cdd:PLN02387 572 GKVEAALSVSPYVDNIMVHADPfHSYCVALVVPSQQALEKWAKKAGIDYSNFAELCEKEEAVKEVQQSLSKAAKAarLEK 651
                        570       580       590       600
                 ....*....|....*....|....*....|....*....|....
gi 544685990 546 FEQVKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMY 589
Cdd:PLN02387 652 FEIPAKIKLLPEPWTPESGLVTAALKLKREQIRKKFKDDLKKLY 695
Firefly_Luc_like cd05911
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ...
34-487 3.20e-62

Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341237 [Multi-domain]  Cd Length: 486  Bit Score: 213.23  E-value: 3.20e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  34 QEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFV 113
Cdd:cd05911    9 KELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVIFT 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 114 gDQEQFDQVYQIANNCPQLMKIVAMKAnmHLRDLPNACYWEDFLNVVPNEAEFEKRLNSKQlsDLFTLIYTSGTTGEPKG 193
Cdd:cd05911   89 -DPDGLEKVKEAAKELGPKDKIIVLDD--KPDGVLSIEDLLSPTLGEEDEDLPPPLKDGKD--DTAAILYSSGTTGLPKG 163
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 194 VMLDYANL---AHQLNAHdLALNVNEDDVSLSFLPLSHIFerawiayvfhrGATNCYLedtkhvrdALTTLKPTVMCavP 270
Cdd:cd05911  164 VCLSHRNLianLSQVQTF-LYGNDGSNDVILGFLPLYHIY-----------GLFTTLA--------SLLNGATVIIM--P 221
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 RFYekiyTAVW-DKVEKaplhrralfnwairvgeqhYQIEQ----PSQWLRL-QYALADKLVLTKLRALLggrikmmpCG 344
Cdd:cd05911  222 KFD----SELFlDLIEK-------------------YKITFlylvPPIAAALaKSPLLDKYDLSSLRVIL--------SG 270
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 345 GAKLEASIGSFFHSIGIN--IKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI-----------GEENEILVRGG 411
Cdd:cd05911  271 GAPLSKELQELLAKRFPNatIKQGYGMTETGGILTVNPDGDDKPGSVGRLLPNVEAKIvdddgkdslgpNEPGEICVRGP 350
                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 412 MVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:cd05911  351 QVMKGYYNNPEATKETFDEDGWLHTGDIGYFDEDGYLYIVDRKKELIKY-KGFQVAPAELEAVLLEHPGVADAAVI 425
PRK07656 PRK07656
long-chain-fatty-acid--CoA ligase; Validated
20-487 1.19e-60

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236072 [Multi-domain]  Cd Length: 513  Bit Score: 209.76  E-value: 1.19e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  20 LNRTALRFREQAQWQEM----SWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNT 95
Cdd:PRK07656  11 LARAARRFGDKEAYVFGdqrlTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLNTRYT 90
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  96 AKQVEYIVNDADIKILFVgdQEQF-DQVYQIANNCPQLMKIVAMKANMHLRDLPNACYWEDFLNvVPNEAEFEKRLNSKQ 174
Cdd:PRK07656  91 ADEAAYILARGDAKALFV--LGLFlGVDYSATTRLPALEHVVICETEEDDPHTEKMKTFTDFLA-AGDPAERAPEVDPDD 167
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 175 LSDlftLIYTSGTTGEPKGVMLDYANLAhqLNAHDLA--LNVNEDDVSLSFLPLSHIF--ERAWIAyVFHRGATnCYLED 250
Cdd:PRK07656 168 VAD---ILFTSGTTGRPKGAMLTHRQLL--SNAADWAeyLGLTEGDRYLAANPFFHVFgyKAGVNA-PLMRGAT-ILPLP 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 251 TKHVRDALTTL---KPTVMCAVPRFYekiytavwdkvekaplhrralfnwairvgeqhyqieqpsQWLrLQYALADKLVL 327
Cdd:PRK07656 241 VFDPDEVFRLIeteRITVLPGPPTMY---------------------------------------NSL-LQHPDRSAEDL 280
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 328 TKLRALLGGRIKMMPcggakleASIGSFFHSIGINIKL-GYGMTETTATVS-CWQDKGFN--PNSIGTLMPNAEVKI--- 400
Cdd:PRK07656 281 SSLRLAVTGAASMPV-------ALLERFESELGVDIVLtGYGLSEASGVTTfNRLDDDRKtvAGTIGTAIAGVENKIvne 353
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEG 473
Cdd:PRK07656 354 lgeevpvGEVGELLVRGPNVMKGYYDDPEATAAAIDADGWLHTGDLGRLDEEGYLYIVDRKKD-MFIVGGFNVYPAEVEE 432
                        490
                 ....*....|....
gi 544685990 474 KIGKDKFIEQIAVI 487
Cdd:PRK07656 433 VLYEHPAVAEAAVI 446
FC-FACS_FadD_like cd05936
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ...
15-487 9.07e-58

Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341259 [Multi-domain]  Cd Length: 468  Bit Score: 200.87  E-value: 9.07e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  15 QAKKWLNRTALRFREQaqwqemsWQTFQQ---EIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:cd05936    8 AARRFPDKTALIFMGR-------KLTYREldaLAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVVVPLN 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  92 ATNTAKQVEYIVNDADIKILFVGDqeqfdqvyqianncpqlmkivamkanmhlrdlpnacYWEDFLnvVPNEAEFEKRLN 171
Cdd:cd05936   81 PLYTPRELEHILNDSGAKALIVAV------------------------------------SFTDLL--AAGAPLGERVAL 122
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 172 SKQlsDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVN--EDDVSLSFLPLSHIFerAWIA---YVFHRGATNC 246
Cdd:cd05936  123 TPE--DVAVLQYTSGTTGVPKGAMLTHRNLVANALQIKAWLEDLleGDDVVLAALPLFHVF--GLTVallLPLALGATIV 198
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 247 YLE--DTKHVRDALTTLKPTVMCAVPRFYEKIytavwdkvekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADK 324
Cdd:cd05936  199 LIPrfRPIGVLKEIRKHRVTIFPGVPTMYIAL----------------------------------------LNAPEFKK 238
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 325 LVLTKLRALLggrikmmpCGGAKLEASIGSFFHSI-GINIKLGYGMTETTATVSC----WQDKgfnPNSIGTLMPNAEVK 399
Cdd:cd05936  239 RDFSSLRLCI--------SGGAPLPVEVAERFEELtGVPIVEGYGLTETSPVVAVnpldGPRK---PGSIGIPLPGTEVK 307
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 400 I----------GEENEILVRGGMVMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQ 469
Cdd:cd05936  308 IvdddgeelppGEVGELWVRGPQVMKGYWNRPEETAEAFV-DGWLRTGDIGYMDEDGYFFIVDRKKD-MIIVGGFNVYPR 385
                        490
                 ....*....|....*...
gi 544685990 470 YIEGKIGKDKFIEQIAVI 487
Cdd:cd05936  386 EVEEVLYEHPAVAEAAVV 403
PTZ00216 PTZ00216
acyl-CoA synthetase; Provisional
177-591 9.24e-58

acyl-CoA synthetase; Provisional


Pssm-ID: 240316 [Multi-domain]  Cd Length: 700  Bit Score: 205.98  E-value: 9.24e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLI-YTSGTTGEPKGVMLDYANLAHQLNAHDLALN-----VNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCY--- 247
Cdd:PTZ00216 264 DDLALImYTSGTTGDPKGVMHTHGSLTAGILALEDRLNdligpPEEDETYCSYLPLAHIMEFGVTNIFLARGALIGFgsp 343
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 248 ---LEDTKHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAirvgeqhYQIeqpsqwlRLQyALAD- 323
Cdd:PTZ00216 344 rtlTDTFARPHGDLTEFRPVFLIGVPRIFDTIKKAVEAKLPPVGSLKRRVFDHA-------YQS-------RLR-ALKEg 408
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 324 -------KLVLTKLRALLGGRIKMMPCGGAKLEASIGSFFHSI-GINIKlGYGMTETTAtVSCWQDKG-FNPNSIGTLMP 394
Cdd:PTZ00216 409 kdtpywnEKVFSAPRAVLGGRVRAMLSGGGPLSAATQEFVNVVfGMVIQ-GWGLTETVC-CGGIQRTGdLEPNAVGQLLK 486
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 395 NAEVKI--GEE----------NEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSN 462
Cdd:PTZ00216 487 GVEMKLldTEEykhtdtpeprGEILLRGPFLFKGYYKQEELTREVLDEDGWFHTGDVGSIAANGTLRIIGRVKALAKNCL 566
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 463 GKYIAPQYIEGKIGKDKFI--EQIAVIAD-AKKYVSALIVPCFDSLEEYAKQLNIK------YQDRIELIKHSDIIQMFE 533
Cdd:PTZ00216 567 GEYIALEALEALYGQNELVvpNGVCVLVHpARSYICALVLTDEAKAMAFAKEHGIEgeypaiLKDPEFQKKATESLQETA 646
                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 534 RRIHElqkelPSFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMYNE 591
Cdd:PTZ00216 647 RAAGR-----KSFEIVRHVRVLSDEWTPENGVLTAAMKLKRRVIDERYADLIKELFAD 699
AFD_class_I cd04433
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ...
177-501 2.23e-56

Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.


Pssm-ID: 341228 [Multi-domain]  Cd Length: 336  Bit Score: 193.27  E-value: 2.23e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLE--DTKHV 254
Cdd:cd04433    1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAASGGLTEGDVFLSTLPLFHIGGLFGLLGALLAGGTVVLLPkfDPEAA 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 255 RDALTTLKPTVMCAVPRFYEKIytavwdkvekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADKLVLTKLRALL 334
Cdd:cd04433   81 LELIEREKVTILLGVPTLLARL----------------------------------------LKAPESAGYDLSSLRALV 120
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 335 ggrikmmpCGGAKLEASIGSFFHSI-GINIKLGYGMTETTATVSCWQ--DKGFNPNSIGTLMPNAEVKI----------G 401
Cdd:cd04433  121 --------SGGAPLPPELLERFEEApGIKLVNGYGLTETGGTVATGPpdDDARKPGSVGRPVPGVEVRIvdpdggelppG 192
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 402 EENEILVRGGMVMRGYYKKPEETAkAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNGKYIAPQYIEGKIGKDKFI 481
Cdd:cd04433  193 EIGELVVRGPSVMKGYWNNPEATA-AVDEDGWYRTGDLGRLDEDGYLYIVGRLKDMIK-SGGENVYPAEVEAVLLGHPGV 270
                        330       340
                 ....*....|....*....|....
gi 544685990 482 EQIAVIA--DAKK--YVSALIVPC 501
Cdd:cd04433  271 AEAAVVGvpDPEWgeRVVAVVVLR 294
FACL_FadD13-like cd17631
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ...
21-500 2.66e-53

fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.


Pssm-ID: 341286 [Multi-domain]  Cd Length: 435  Bit Score: 187.82  E-value: 2.66e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:cd17631   10 DRTALVFGGR----SLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTPPEVA 85
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFvgdqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqlSDLFT 180
Cdd:cd17631   86 YILADSGAKVLF---------------------------------------------------------------DDLAL 102
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFE-RAWIAYVFHRGATNCYLE--DTKHVRDA 257
Cdd:cd17631  103 LMYTSGTTGRPKGAMLTHRNLLWNAVNALAALDLGPDDVLLVVAPLFHIGGlGVFTLPTLLRGGTVVILRkfDPETVLDL 182
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 258 LTTLKPTVMCAVPrfyekiytAVWDKVekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADKLVLTKLRALLggr 337
Cdd:cd17631  183 IERHRVTSFFLVP--------TMIQAL--------------------------------LQHPRFATTDLSSLRAVI--- 219
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 338 ikmmpCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVsCW------QDKgfnPNSIGTLMPNAEVKI----------G 401
Cdd:cd17631  220 -----YGGAPMPERLLRALQARGVKFVQGYGMTETSPGV-TFlspedhRRK---LGSAGRPVFFVEVRIvdpdgrevppG 290
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 402 EENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDKFI 481
Cdd:cd17631  291 EVGEIVVRGPHVMAGYWNRPEATAAAF-RDGWFHTGDLGRLDEDGYLYIVDRKKD-MIISGGENVYPAEVEDVLYEHPAV 368
                        490       500
                 ....*....|....*....|...
gi 544685990 482 EQIAVIA--DAK--KYVSALIVP 500
Cdd:cd17631  369 AEVAVIGvpDEKwgEAVVAVVVP 391
PRK08314 PRK08314
long-chain-fatty-acid--CoA ligase; Validated
16-500 1.66e-45

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236235 [Multi-domain]  Cd Length: 546  Bit Score: 168.99  E-value: 1.66e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  16 AKKWLNRTALRFREQAqwqeMSWQTFQQEIDRFSyALIAQHIDIQ--DKIGIFANNMPRWTIADFGAMQARAVAVPIYAT 93
Cdd:PRK08314  20 ARRYPDKTAIVFYGRA----ISYRELLEEAERLA-GYLQQECGVRkgDRVLLYMQNSPQFVIAYYAILRANAVVVPVNPM 94
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  94 NTAKQVEYIVNDADIKILFVGdQEQFDQVYQIANNCPQLMKIVA-----------------MKANMHLRDLPN--ACYWE 154
Cdd:PRK08314  95 NREEELAHYVTDSGARVAIVG-SELAPKVAPAVGNLRLRHVIVAqysdylpaepeiavpawLRAEPPLQALAPggVVAWK 173
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 155 DFL--NVVPNEAEfekrlnsKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHI--F 230
Cdd:PRK08314 174 EALaaGLAPPPHT-------AGPDDLAVLPYTSGTTGVPKGCMHTHRTVMANAVGSVLWSNSTPESVVLAVLPLFHVtgM 246
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 231 ERAWIAYVFhRGATncyledtkhvrdalttlkpTVMcaVPRfyekiytavWDKvEKAplhRRALfnwairvgeQHYQIeq 310
Cdd:PRK08314 247 VHSMNAPIY-AGAT-------------------VVL--MPR---------WDR-EAA---ARLI---------ERYRV-- 280
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 311 pSQWLRLQYALADKLV--------LTKLRaLLGGrikmmpcGGAKLEASIGSFFHSI-GINIKLGYGMTETTATVScwqd 381
Cdd:PRK08314 281 -THWTNIPTMVVDFLAspglaerdLSSLR-YIGG-------GGAAMPEAVAERLKELtGLDYVEGYGLTETMAQTH---- 347
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 382 kgFNP------NSIG--------------TLmpnAEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTE-DG--FLRTGD 438
Cdd:PRK08314 348 --SNPpdrpklQCLGiptfgvdarvidpeTL---EELPPGEVGEIVVHGPQVFKGYWNRPEATAEAFIEiDGkrFFRTGD 422
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 439 VGEMDSCGNLFITDRLKELMKTSNGKyIAPQYIEGKIGKDKFIEQIAVIA--DAKK--YVSALIVP 500
Cdd:PRK08314 423 LGRMDEEGYFFITDRLKRMINASGFK-VWPAEVENLLYKHPAIQEACVIAtpDPRRgeTVKAVVVL 487
4CL cd05904
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ...
47-468 7.26e-44

4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.


Pssm-ID: 341230 [Multi-domain]  Cd Length: 505  Bit Score: 163.56  E-value: 7.26e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  47 RFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgDQEQFDQVYQIA 126
Cdd:cd05904   44 RLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLSTPAEIAKQVKDSGAKLAFT-TAELAEKLASLA 122
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 127 NncpqlmKIVAMkanmhlRDLPNACYWEDFLNVVPNEAEFeKRLNSKQlSDLFTLIYTSGTTGEPKGVMLDYANL---AH 203
Cdd:cd05904  123 L------PVVLL------DSAEFDSLSFSDLLFEADEAEP-PVVVIKQ-DDVAALLYSSGTTGRSKGVMLTHRNLiamVA 188
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 204 QLNAhDLALNVNEDDVSLSFLPLSHIFERAWIAY-VFHRGATNCYLE--DTKHVRDALTTLKPTVMCAVPrfyeKIYTAV 280
Cdd:cd05904  189 QFVA-GEGSNSDSEDVFLCVLPMFHIYGLSSFALgLLRLGATVVVMPrfDLEELLAAIERYKVTHLPVVP----PIVLAL 263
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 281 WDKvekaplhrralfnwairvgeqhyqieqpsqwlrlqyALADKLVLTKLRALLggrikmmpCGGAKLEASIGSFFHSI- 359
Cdd:cd05904  264 VKS------------------------------------PIVDKYDLSSLRQIM--------SGAAPLGKELIEAFRAKf 299
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 360 -GINIKLGYGMTETTA-TVSCWQDKGF--NPNSIGTLMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEET 424
Cdd:cd05904  300 pNVDLGQGYGMTESTGvVAMCFAPEKDraKYGSVGRLVPNVEAKIvdpetgeslppNQTGELWIRGPSIMKGYLNNPEAT 379
                        410       420       430       440
                 ....*....|....*....|....*....|....*....|....
gi 544685990 425 AKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAP 468
Cdd:cd05904  380 AATIDKEGWLHTGDLCYIDEDGYLFIVDRLKELIKY-KGFQVAP 422
LC_FACS_like cd05935
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ...
35-499 2.17e-42

Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.


Pssm-ID: 341258 [Multi-domain]  Cd Length: 430  Bit Score: 158.03  E-value: 2.17e-42
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  35 EMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVG 114
Cdd:cd05935    1 SLTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVG 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 115 dqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnsKQLSDLFTLIYTSGTTGEPKGV 194
Cdd:cd05935   81 ----------------------------------------------------------SELDDLALIPYTSGTTGLPKGC 102
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 195 MLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHI--FERAWIAYVFhRGATNCYLE--DTKHVRDALTTLKPTVMCAVP 270
Cdd:cd05935  103 MHTHFSAAANALQSAVWTGLTPSDVILACLPLFHVtgFVGSLNTAVY-VGGTYVLMArwDRETALELIEKYKVTFWTNIP 181
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 rfyekiyTAVWDKVEKAPLHRRALfnwairvgeqhyqieqpsqwlrlqyaladklvlTKLRALLGGRIKMMPCGGAKLEA 350
Cdd:cd05935  182 -------TMLVDLLATPEFKTRDL---------------------------------SSLKVLTGGGAPMPPAVAEKLLK 221
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 351 SIGSFFHSiginiklGYGMTETTATV----------SCWQDKGFNPNS-IGTLMPNAEVKIGEENEILVRGGMVMRGYYK 419
Cdd:cd05935  222 LTGLRFVE-------GYGLTETMSQThtnpplrpklQCLGIP*FGVDArVIDIETGRELPPNEVGEIVVRGPQIFKGYWN 294
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 420 KPEETAKAFTEDG---FLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVIADAKKY--- 493
Cdd:cd05935  295 RPEETEESFIEIKgrrFFRTGDLGYMDEEGYFFFVDRVKRMINVS-GFKVWPAEVEAKLYKHPAI*EVCVISVPDERvge 373

                 ....*..
gi 544685990 494 -VSALIV 499
Cdd:cd05935  374 eVKAFIV 380
FACL_DitJ_like cd05934
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
34-488 2.49e-38

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.


Pssm-ID: 341257 [Multi-domain]  Cd Length: 422  Bit Score: 146.28  E-value: 2.49e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  34 QEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFV 113
Cdd:cd05934    2 RRWTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVVV 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 114 gdqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqlsDLFTLIYTSGTTGEPKG 193
Cdd:cd05934   82 ---------------------------------------------------------------DPASILYTSGTTGPPKG 98
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 194 VMLDYANLAH--QLNAHDLALNvnEDDVSLSFLPLSHIFERAWIAYV-FHRGATnCYLEDTKHVRdalttlkptvmcavp 270
Cdd:cd05934   99 VVITHANLTFagYYSARRFGLG--EDDVYLTVLPLFHINAQAVSVLAaLSVGAT-LVLLPRFSAS--------------- 160
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 RFyekiytavWDKVEKaplHRRALFNWAIRVGEqhYQIEQPSQWLRLQYaladklvltKLRALlggrikmmpCGGAKLEA 350
Cdd:cd05934  161 RF--------WSDVRR---YGATVTNYLGAMLS--YLLAQPPSPDDRAH---------RLRAA---------YGAPNPPE 209
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 351 SIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI----------GEENEILVRG----GMvMRG 416
Cdd:cd05934  210 LHEEFEERFGVRLLEGYGMTETIVGVIGPRDEPRRPGSIGRPAPGYEVRIvdddgqelpaGEPGELVIRGlrgwGF-FKG 288
                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 544685990 417 YYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:cd05934  289 YYNMPEATAEAM-RNGWFHTGDLGYRDADGFFYFVDRKKDMIRRR-GENISSAEVERAILRHPAVREAAVVA 358
Acs COG0365
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
21-472 9.17e-38

Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];


Pssm-ID: 440134 [Multi-domain]  Cd Length: 565  Bit Score: 147.18  E-value: 9.17e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  21 NRTALRFR-EQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQV 99
Cdd:COG0365   24 DKVALIWEgEDGEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVIAMLACARIGAVHSPVFPGFGAEAL 103
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 100 EYIVNDADIKILFVGD--------QEQFDQVYQIANNCPQLMKIVAMKANMHLRDLPNACYWEDFLNVVPNEAEFEkRLN 171
Cdd:COG0365  104 ADRIEDAEAKVLITADgglrggkvIDLKEKVDEALEELPSLEHVIVVGRTGADVPMEGDLDWDELLAAASAEFEPE-PTD 182
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 172 SkqlSDLFTLIYTSGTTGEPKGVMLDYAN-LAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAY-VFHRGATNCYLE 249
Cdd:COG0365  183 A---DDPLFILYTSGTTGKPKGVVHTHGGyLVHAATTAKYVLDLKPGDVFWCTADIGWATGHSYIVYgPLLNGATVVLYE 259
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 DTKHVRDALTTL------KPTVMCAVPRFYekiytavwdkvekaplhrRALFNWAIRVGEQHyqieqpsqwlrlqyalaD 323
Cdd:COG0365  260 GRPDFPDPGRLWeliekyGVTVFFTAPTAI------------------RALMKAGDEPLKKY-----------------D 304
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 324 klvLTKLRALLggrikmmpCGGAKLEASIGSFFHS-IGINIKLGYGMTETTAT-VSCWQDKGFNPNSIGTLMPNAEVKI- 400
Cdd:COG0365  305 ---LSSLRLLG--------SAGEPLNPEVWEWWYEaVGVPIVDGWGQTETGGIfISNLPGLPVKPGSMGKPVPGYDVAVv 373
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 ---------GEENEILVRG---GMvMRGYYKKPEETAKAF--TEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYI 466
Cdd:COG0365  374 dedgnpvppGEEGELVIKGpwpGM-FRGYWNDPERYRETYfgRFPGWYRTGDGARRDEDGYFWILGRSDDVINVS-GHRI 451

                 ....*.
gi 544685990 467 APQYIE 472
Cdd:COG0365  452 GTAEIE 457
ttLC_FACS_AlkK_like cd12119
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ...
37-488 1.15e-37

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.


Pssm-ID: 341284 [Multi-domain]  Cd Length: 518  Bit Score: 146.24  E-value: 1.15e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  37 SWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgDQ 116
Cdd:cd12119   27 TYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHTINPRLFPEQIAYIINHAEDRVVFV-DR 105
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 117 EQFDQVYQIANNCPQLMKIVAM--KANMHLRDLPNACYWEDFLNVVPNEAEFeKRLNSKQLSdlfTLIYTSGTTGEPKGV 194
Cdd:cd12119  106 DFLPLLEAIAPRLPTVEHVVVMtdDAAMPEPAGVGVLAYEELLAAESPEYDW-PDFDENTAA---AICYTSGTTGNPKGV 181
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 195 MLDYANLAHQ---LNAHDlALNVNEDDVSLSFLPLSHIfeRAW-IAYV-FHRGA----TNCYLeDTKHVRDALTTLKPTV 265
Cdd:cd12119  182 VYSHRSLVLHamaALLTD-GLGLSESDVVLPVVPMFHV--NAWgLPYAaAMVGAklvlPGPYL-DPASLAELIEREGVTF 257
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 266 MCAVPrfyekiytAVWDKVekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADKLVLTKLRALLggrikmmpCGG 345
Cdd:cd12119  258 AAGVP--------TVWQGL--------------------------------LDHLEANGRDLSSLRRVV--------IGG 289
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 346 AKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPN-----------SIGTLMPNAEVKIGEEN---------- 404
Cdd:cd12119  290 SAVPRSLIEAFEERGVRVIHAWGMTETSPLGTVARPPSEHSNlsedeqlalraKQGRPVPGVELRIVDDDgrelpwdgka 369
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 --EILVRGGMVMRGYYKKPEETAkAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNGKYIAPQYIEGKIGKDKFIE 482
Cdd:cd12119  370 vgELQVRGPWVTKSYYKNDEESE-ALTEDGWLRTGDVATIDEDGYLTITDRSKDVIK-SGGEWISSVELENAIMAHPAVA 447

                 ....*.
gi 544685990 483 QIAVIA 488
Cdd:cd12119  448 EAAVIG 453
PTZ00342 PTZ00342
acyl-CoA synthetase; Provisional
180-466 2.66e-37

acyl-CoA synthetase; Provisional


Pssm-ID: 240370 [Multi-domain]  Cd Length: 746  Bit Score: 147.56  E-value: 2.66e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 180 TLIYTSGTTGEPKGVMLDYANLAHQ---LNAHDLALNVNEDDvSLSFLPLSHIFERAWIAYVFHRGAT-NCYLEDTKHVR 255
Cdd:PTZ00342 308 SIVYTSGTSGKPKGVMLSNKNLYNTvvpLCKHSIFKKYNPKT-HLSYLPISHIYERVIAYLSFMLGGTiNIWSKDINYFS 386
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 256 DALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAIRVGEQHYQIEQpSQWLrlqyalaDKL--VLTKLRAL 333
Cdd:PTZ00342 387 KDIYNSKGNILAGVPKVFNRIYTNIMTEINNLPPLKRFLVKKILSLRKSNNNGGF-SKFL-------EGIthISSKIKDK 458
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 334 LGGRIKMMPCGGAKLEASIGSFFhSIGINIKL--GYGMTETTATVSCWQDKGFNPNSIG-TLMPNAEVKIGE-------- 402
Cdd:PTZ00342 459 VNPNLEVILNGGGKLSPKIAEEL-SVLLNVNYyqGYGLTETTGPIFVQHADDNNTESIGgPISPNTKYKVRTwetykatd 537
                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990 403 ---ENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYI 466
Cdd:PTZ00342 538 tlpKGELLIKSDSIFSGYFLEKEQTKNAFTEDGYFKTGDIVQINKNGSLTFLDRSKGLVKLSQGEYI 604
MCS cd05941
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ...
21-516 1.11e-36

Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.


Pssm-ID: 341264 [Multi-domain]  Cd Length: 442  Bit Score: 142.04  E-value: 1.11e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQ-DKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQV 99
Cdd:cd05941    1 DRIAIVDDGD----SITYADLVARAARLANRLLALGKDLRgDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLAEL 76
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 100 EYIVNDADIKILFvgdqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqlsDLF 179
Cdd:cd05941   77 EYVITDSEPSLVL----------------------------------------------------------------DPA 92
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 180 TLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHI-----------FERAWIayVFHRGATNCYL 248
Cdd:cd05941   93 LILYTSGTTGRPKGVVLTHANLAANVRALVDAWRWTEDDVLLHVLPLHHVhglvnallcplFAGASV--EFLPKFDPKEV 170
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 249 EDTKHVRDAlttlkpTVMCAVPRFYEKIytavwdkvekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADKLVLT 328
Cdd:cd05941  171 AISRLMPSI------TVFMGVPTIYTRL----------------------------------------LQYYEAHFTDPQ 204
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 KLRALLGGRIKMMPCGGAKLEASIGSFFHSIGINIKLG-YGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKIGEEN--- 404
Cdd:cd05941  205 FARAAAAERLRLMVSGSAALPVPTLEEWEAITGHTLLErYGMTEIGMALSNPLDGERRPGTVGMPLPGVQARIVDEEtge 284
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 --------EILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKYIAPQYIEGKIG 476
Cdd:cd05941  285 plprgevgEIQVRGPSVFKEYWNKPEATKEEFTDDGWFKTGDLGVVDEDGYYWILGRSSVDIIKSGGYKVSALEIERVLL 364
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|.
gi 544685990 477 KDKFIEQIAVI----ADAKKYVSALIVP-------CFDSLEEYAKQLNIKY 516
Cdd:cd05941  365 AHPGVSECAVIgvpdPDWGERVVAVVVLragaaalSLEELKEWAKQRLAPY 415
ligase_PEP_1 TIGR03098
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ...
23-472 6.58e-35

acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.


Pssm-ID: 211788 [Multi-domain]  Cd Length: 517  Bit Score: 138.38  E-value: 6.58e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   23 TALRFREQAqwqeMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYI 102
Cdd:TIGR03098  17 TALVHHDRT----LTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGVFVPINPLLKAEQVAHI 92
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  103 VNDADIKILfVGDQEQFDQVYQIANNCPQLMKIVAMKANMHLRDLP---NACYWEDFLNVVPneaefEKRLNSKQLSDLF 179
Cdd:TIGR03098  93 LADCNVRLL-VTSSERLDLLHPALPGCHDLRTLIIVGDPAHASEGHpgeEPASWPKLLALGD-----ADPPHPVIDSDMA 166
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  180 TLIYTSGTTGEPKGVMLDYANLAhqLNAHDLA--LNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATnCYLEDTKHVRDA 257
Cdd:TIGR03098 167 AILYTSGSTGRPKGVVLSHRNLV--AGAQSVAtyLENRPDDRLLAVLPLSFDYGFNQLTTAFYVGAT-VVLHDYLLPRDV 243
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  258 LTTLKP---TVMCAVPrfyekiytavwdkvekaPLhrralfnWAirvgeqhyqieqpsQWLRLQYALADKLVLTKLrALL 334
Cdd:TIGR03098 244 LKALEKhgiTGLAAVP-----------------PL-------WA--------------QLAQLDWPESAAPSLRYL-TNS 284
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  335 GGRikmMPcggAKLEASIGSFFHSigINIKLGYGMTEttATVSCWQDKGF---NPNSIGTLMPNAEVKI----------G 401
Cdd:TIGR03098 285 GGA---MP---RATLSRLRSFLPN--ARLFLMYGLTE--AFRSTYLPPEEvdrRPDSIGKAIPNAEVLVlredgsecapG 354
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  402 EENEILVRGGMVMRGYYKKPEETAKAFT-----EDGFLRT------GDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQY 470
Cdd:TIGR03098 355 EEGELVHRGALVAMGYWNDPEKTAERFRplppfPGELHLPelavwsGDTVRRDEEGFLYFVGRRDEMIKTS-GYRVSPTE 433

                  ..
gi 544685990  471 IE 472
Cdd:TIGR03098 434 VE 435
FACL_fum10p_like cd05926
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ...
37-579 3.47e-34

Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.


Pssm-ID: 341249 [Multi-domain]  Cd Length: 493  Bit Score: 135.90  E-value: 3.47e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  37 SWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQ 116
Cdd:cd05926   16 TYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSKLVLTPKG 95
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 117 EQFDqvyqianncpqlmkivAMKANMHLRDLPNACYWEDFLNVVPNEAE------FEKRLNSKQL----SDLFTLIYTSG 186
Cdd:cd05926   96 ELGP----------------ASRAASKLGLAILELALDVGVLIRAPSAEslsnllADKKNAKSEGvplpDDLALILHTSG 159
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 187 TTGEPKGVMLDYANLA---------HQLNAHDLALNVneddvslsfLPLSHIferawiayvfhrgatncyledtkH--VR 255
Cdd:cd05926  160 TTGRPKGVPLTHRNLAasatnitntYKLTPDDRTLVV---------MPLFHV-----------------------HglVA 207
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 256 DALTTL--KPTVMCAvPRFYEkiyTAVWDKVEKAplhrRAlfNWAIRVGEQHyQIeqpsqwlRLQYALADKL-VLTKLRA 332
Cdd:cd05926  208 SLLSTLaaGGSVVLP-PRFSA---STFWPDVRDY----NA--TWYTAVPTIH-QI-------LLNRPEPNPEsPPPKLRF 269
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 333 LLGGRIKMMPCGGAKLEAsigsFFhsiGINIKLGYGMTETTATVSC-----WQDKgfnPNSIGtlMPN-AEVKI------ 400
Cdd:cd05926  270 IRSCSASLPPAVLEALEA----TF---GAPVLEAYGMTEAAHQMTSnplppGPRK---PGSVG--KPVgVEVRIldedge 337
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 ----GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIG 476
Cdd:cd05926  338 ilppGVVGEICLRGPNVTRGYLNNPEANAEAAFKDGWFRTGDLGYLDADGYLFLTGRIKELINRG-GEKISPLEVDGVLL 416
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 477 KDKFIEQIAVIA--DAkKY---VSALIVPCFDSleeyakqlnikYQDRIELIKHsdiiqmferriheLQKELPSFEQVKR 551
Cdd:cd05926  417 SHPAVLEAVAFGvpDE-KYgeeVAAAVVLREGA-----------SVTEEELRAF-------------CRKHLAAFKVPKK 471
                        570       580       590
                 ....*....|....*....|....*....|.
gi 544685990 552 FTL---LPQafstameeiTPTLKLRRKVIMQ 579
Cdd:cd05926  472 VYFvdeLPK---------TATGKIQRRKVAE 493
PRK06145 PRK06145
acyl-CoA synthetase; Validated
2-499 5.97e-34

acyl-CoA synthetase; Validated


Pssm-ID: 102207 [Multi-domain]  Cd Length: 497  Bit Score: 135.40  E-value: 5.97e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   2 ASLDFHfvnrfrlqAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAM 81
Cdd:PRK06145   6 ASIAFH--------ARRTPDRAALVYRDQ----EISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAAS 73
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  82 QARAVAVPIYATNTAKQVEYIVNDADIKILFVgdQEQFDQVyqIANNCPQLMKIVAMKANMHLRDLPNAcywedflNVVP 161
Cdd:PRK06145  74 YLGAVFLPINYRLAADEVAYILGDAGAKLLLV--DEEFDAI--VALETPKIVIDAAAQADSRRLAQGGL-------EIPP 142
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 162 NEAEFEkrlnskqlSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHI--FERAWIAYVF 239
Cdd:PRK06145 143 QAAVAP--------TDLVRLMYTSGTTDRPKGVMHSYGNLHWKSIDHVIALGLTASERLLVVGPLYHVgaFDLPGIAVLW 214
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 240 HrGATNCYLEDTKhvrdalttlKPTVMCAVPRfyEKIyTAVWdkveKAPLHRRALFNWAIRvgeqhyqieqpsqwlrlqy 319
Cdd:PRK06145 215 V-GGTLRIHREFD---------PEAVLAAIER--HRL-TCAW----MAPVMLSRVLTVPDR------------------- 258
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 320 alaDKLVLTKLRALLGGrikmmpcgGAKLEASIGSFFHSIGINIKL--GYGMTETTATvSCWQDKGFNPNSIGTL---MP 394
Cdd:PRK06145 259 ---DRFDLDSLAWCIGG--------GEKTPESRIRDFTRVFTRARYidAYGLTETCSG-DTLMEAGREIEKIGSTgraLA 326
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 395 NAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGK 464
Cdd:PRK06145 327 HVEIRIadgagrwlppNMKGEICMRGPKVTKGYWKDPEKTAEAFY-GDWFRSGDVGYLDEEGFLYLTDRKKD-MIISGGE 404
                        490       500       510
                 ....*....|....*....|....*....|....*....
gi 544685990 465 YIAPQYIEGKIGKDKFIEQIAVIA--DAK--KYVSALIV 499
Cdd:PRK06145 405 NIASSEVERVIYELPEVAEAAVIGvhDDRwgERITAVVV 443
AAS_C cd05909
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ...
75-501 6.74e-34

C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.


Pssm-ID: 341235 [Multi-domain]  Cd Length: 490  Bit Score: 135.15  E-value: 6.74e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  75 IADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQ--EQFDQvyqianncPQLMKIVAMKANMHLRDLPNACY 152
Cdd:cd05909   46 LANFALALSGKVPVMLNYTAGLRELRACIKLAGIKTVLTSKQfiEKLKL--------HHLFDVEYDARIVYLEDLRAKIS 117
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 153 WED----FL--NVVPNEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPL 226
Cdd:cd05909  118 KADkckaFLagKFPPKWLLRIFGVAPVQPDDPAVILFTSGSEGLPKGVVLSHKNLLANVEQITAIFDPNPEDVVFGALPF 197
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 227 SHIFERA---W------IAYVFHRGATncyleDTKHVRDALTTLKPTVMCAVPRFYeKIYTavwdkvekaplhRRAlfnw 297
Cdd:cd05909  198 FHSFGLTgclWlpllsgIKVVFHPNPL-----DYKKIPELIYDKKATILLGTPTFL-RGYA------------RAA---- 255
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 298 airvgeqhyqieQPSQWLRLQYALadklvltklrallggrikmmpCGGAKLEASIGSFFHS-IGINIKLGYGMTETTATV 376
Cdd:cd05909  256 ------------HPEDFSSLRLVV---------------------AGAEKLKDTLRQEFQEkFGIRILEGYGTTECSPVI 302
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 377 SCWQDK-GFNPNSIGTLMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDS 444
Cdd:cd05909  303 SVNTPQsPNKEGTVGRPLPGMEVKIvsvetheevpiGEGGLLLVRGPNVMLGYLNEPELTSFAF-GDGWYDTGDIGKIDG 381
                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 445 CGNLFITDRLKELMKtsngkyiapqyIEGKIGKDKFIEQIA-VIADAKKYVSALIVPC 501
Cdd:cd05909  382 EGFLTITGRLSRFAK-----------IAGEMVSLEAIEDILsEILPEDNEVAVVSVPD 428
Firefly_Luc cd17642
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ...
35-487 1.11e-33

insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.


Pssm-ID: 341297 [Multi-domain]  Cd Length: 532  Bit Score: 134.96  E-value: 1.11e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  35 EMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVg 114
Cdd:cd17642   44 NYSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNERELDHSLNISKPTIVFC- 122
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 115 DQEQFDQVYQIANNCPQLMKIVAMKANMHLRDLPNAcywEDFL--NVVPNEAEFEKRLNSKQLSDLFTLI-YTSGTTGEP 191
Cdd:cd17642  123 SKKGLQKVLNVQKKLKIIKTIIILDSKEDYKGYQCL---YTFItqNLPPGFNEYDFKPPSFDRDEQVALImNSSGSTGLP 199
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 192 KGVMLDYANLAHQLN-AHD--LALNVNEDDVSLSFLPLSHIFerawiayvfhrgatncyledtkhvrdALTTLKPTVMCA 268
Cdd:cd17642  200 KGVQLTHKNIVARFShARDpiFGNQIIPDTAILTVIPFHHGF--------------------------GMFTTLGYLICG 253
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 269 -----VPRFYEKIYTavwdkvekaplhrRALfnwairvgeQHYQIEQ----PSQWLRL-QYALADKLVLTKLRALLggri 338
Cdd:cd17642  254 frvvlMYKFEEELFL-------------RSL---------QDYKVQSallvPTLFAFFaKSTLVDKYDLSNLHEIA---- 307
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 339 kmmpCGGAKLEASIGSFF-HSIGIN-IKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI-----------GEENE 405
Cdd:cd17642  308 ----SGGAPLSKEVGEAVaKRFKLPgIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFYAKVvdldtgktlgpNERGE 383
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 406 ILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGKIGKDKFIEQIA 485
Cdd:cd17642  384 LCVKGPMIMKGYVNNPEATKALIDKDGWLHSGDIAYYDEDGHFFIVDRLKSLIKY-KGYQVPPAELESILLQHPKIFDAG 462

                 ..
gi 544685990 486 VI 487
Cdd:cd17642  463 VA 464
PRK12583 PRK12583
acyl-CoA synthetase; Provisional
21-512 4.61e-33

acyl-CoA synthetase; Provisional


Pssm-ID: 237145 [Multi-domain]  Cd Length: 558  Bit Score: 133.36  E-value: 4.61e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  21 NRTALRFREQAQwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:PRK12583  33 DREALVVRHQAL--RYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVNINPAYRASELE 110
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFVGD----------------QEQFDQVYQIAN-NCPQLMKIVAMKANmhlrDLPNACYWEDFL---NVV 160
Cdd:PRK12583 111 YALGQSGVRWVICADafktsdyhamlqellpGLAEGQPGALACeRLPELRGVVSLAPA----PPPGFLAWHELQargETV 186
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 161 PNEAEFEkRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFER--AWIAYV 238
Cdd:PRK12583 187 SREALAE-RQASLDRDDPINIQYTSGTTGFPKGATLSHHNILNNGYFVAESLGLTEHDRLCVPVPLYHCFGMvlANLGCM 265
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 239 FHrGATNCYLEDTkhvRDALTTLKP------TVMCAVPrfyeKIYTAvwdkvekaplhrralfnwairvgeqhyQIEQPs 312
Cdd:PRK12583 266 TV-GACLVYPNEA---FDPLATLQAveeercTALYGVP----TMFIA---------------------------ELDHP- 309
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 313 qwlrlQYALADklvLTKLR-ALLGGrikmMPCGGAKLEASIGSFFHSigiNIKLGYGMTETT--ATVSCWQDK-GFNPNS 388
Cdd:PRK12583 310 -----QRGNFD---LSSLRtGIMAG----APCPIEVMRRVMDEMHMA---EVQIAYGMTETSpvSLQTTAADDlERRVET 374
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 389 IGTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKElM 458
Cdd:PRK12583 375 VGRTQPHLEVKVvdpdgatvprGEIGELCTRGYSVMKGYWNNPEATAESIDEDGWMHTGDLATMDEQGYVRIVGRSKD-M 453
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....
gi 544685990 459 KTSNGKYIAPQYIEgkigkDKFIEQIAVIAdakkyVSALIVPCfdslEEYAKQL 512
Cdd:PRK12583 454 IIRGGENIYPREIE-----EFLFTHPAVAD-----VQVFGVPD----EKYGEEI 493
FACL_like_6 cd05922
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
84-489 3.51e-32

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341246 [Multi-domain]  Cd Length: 457  Bit Score: 129.48  E-value: 3.51e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  84 RAVAVPIYATNTAKQVEYIVNDADIKILfvgdqeqfdqvyqianncpqlmkIVAMKANMHLRDLPNACY----WEDFLNV 159
Cdd:cd05922   46 GLVFVPLNPTLKESVLRYLVADAGGRIV-----------------------LADAGAADRLRDALPASPdpgtVLDADGI 102
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 160 VPNEAEFEKRLNSKQlsDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVF 239
Cdd:cd05922  103 RAARASAPAHEVSHE--DLALLLYTSGSTGSPKLVRLSHQNLLANARSIAEYLGITADDRALTVLPLSYDYGLSVLNTHL 180
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 240 HRGAT----NCYLEDTKHVrDALTTLKPTVMCAVPRFYEKIYTAVWDKvEKAPlHRRALFNWAIRVgeqhyqieqPSQWL 315
Cdd:cd05922  181 LRGATlvltNDGVLDDAFW-EDLREHGATGLAGVPSTYAMLTRLGFDP-AKLP-SLRYLTQAGGRL---------PQETI 248
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 316 RlqyALADKLVltklrallGGRIKMMpcggakleasigsffhsiginiklgYGMTETTATVSCW--QDKGFNPNSIGTLM 393
Cdd:cd05922  249 A---RLRELLP--------GAQVYVM-------------------------YGQTEATRRMTYLppERILEKPGSIGLAI 292
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 394 PNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnG 463
Cdd:cd05922  293 PGGEFEIldddgtptppGEPGEIVHRGPNVMKGYWNDPPYRRKEGRGGGVLHTGDLARRDEDGFLFIVGRRDRMIKLF-G 371
                        410       420
                 ....*....|....*....|....*.
gi 544685990 464 KYIAPQYIEGKIGKDKFIEQIAVIAD 489
Cdd:cd05922  372 NRISPTEIEAAARSIGLIIEAAAVGL 397
AFD_CAR-like cd17632
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ...
54-508 4.18e-31

adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.


Pssm-ID: 341287 [Multi-domain]  Cd Length: 588  Bit Score: 127.96  E-value: 4.18e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  54 AQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgDQEQFDQVYQIANNCPQLM 133
Cdd:cd17632   87 EQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAPILAETEPRLLAV-SAEHLDLAVEAVLEGGTPP 165
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 134 KIVAM--------------KANMHLRDLPNACYWEDFLNVVPNEAEFEKRLNSKQLSD-LFTLIYTSGTTGEPKGVMLDY 198
Cdd:cd17632  166 RLVVFdhrpevdahraaleSARERLAAVGIPVTTLTLIAVRGRDLPPAPLFRPEPDDDpLALLIYTSGSTGTPKGAMYTE 245
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 199 ANLAHQLnahdLALNVNEDD-----VSLSFLPLSHIFERAWIAYVFHRGATNCYL--EDTKHVRDALTTLKPTVMCAVPR 271
Cdd:cd17632  246 RLVATFW----LKVSSIQDIrppasITLNFMPMSHIAGRISLYGTLARGGTAYFAaaSDMSTLFDDLALVRPTELFLVPR 321
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 272 fyekiytaVWDKVekaplhrralfnwairvgEQHYQIEQpsQWLRLQYALADKL---VLTKLRA-LLGGRIKMMPCGGAK 347
Cdd:cd17632  322 --------VCDML------------------FQRYQAEL--DRRSVAGADAETLaerVKAELRErVLGGRLLAAVCGSAP 373
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 348 LEASIGSFFHS-IGINIKLGYGMTETtatvscwqdkgfnpnsiGTLMPNAEVK--------------IG--------EEN 404
Cdd:cd17632  374 LSAEMKAFMESlLDLDLHDGYGSTEA-----------------GAVILDGVIVrppvldyklvdvpeLGyfrtdrphPRG 436
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 EILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVgeMDSCG--NLFITDRLKELMKTSNGKYIAPQYIEGKIGKDKFIE 482
Cdd:cd17632  437 ELLVKTDTLFPGYYKRPEVTAEVFDEDGFYRTGDV--MAELGpdRLVYVDRRNNVLKLSQGEFVTVARLEAVFAASPLVR 514
                        490       500
                 ....*....|....*....|....*..
gi 544685990 483 QIAVIAD-AKKYVSALIVPCFDSLEEY 508
Cdd:cd17632  515 QIFVYGNsERAYLLAVVVPTQDALAGE 541
A_NRPS cd05930
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ...
21-575 9.47e-31

The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341253 [Multi-domain]  Cd Length: 444  Bit Score: 124.95  E-value: 9.47e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:cd05930    2 DAVAVVDGDQ----SLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERLA 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFVgdqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskQLSDLFT 180
Cdd:cd05930   78 YILEDSGAKLVLT------------------------------------------------------------DPDDLAY 97
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS---HIFE--RAWIAyvfhrGATnCYLEDTKHVR 255
Cdd:cd05930   98 VIYTSGSTGKPKGVMVEHRGLVNLLLWMQEAYPLTPGDRVLQFTSFSfdvSVWEifGALLA-----GAT-LVVLPEEVRK 171
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 256 D------ALTTLKPTVMCAVPRFYekiytavwdkvekaplhrRALFNWAirvgeqhyqieqpsqwlrlqyalaDKLVLTK 329
Cdd:cd05930  172 DpealadLLAEEGITVLHLTPSLL------------------RLLLQEL------------------------ELAALPS 209
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 330 LRALLggrikmmpCGGAKLEASIGSFFHSIGINIKL--GYGMTETTATVSCW----QDKGFNPNSIGTLMPNAE------ 397
Cdd:cd05930  210 LRLVL--------VGGEALPPDLVRRWRELLPGARLvnLYGPTEATVDATYYrvppDDEEDGRVPIGRPIPNTRvyvlde 281
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 398 ----VKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFL------RTGDVGEMDSCGNLFITDRLKELMKTsNGKYIA 467
Cdd:cd05930  282 nlrpVPPGVPGELYIGGAGLARGYLNRPELTAERFVPNPFGpgermyRTGDLVRWLPDGNLEFLGRIDDQVKI-RGYRIE 360
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 468 PQYIEGKIGKDKFIEQIAVIA----DAKKYVSALIVPcfdsleeyakqlnikyqDRIELIKHSDIIQmferrihELQKEL 543
Cdd:cd05930  361 LGEIEAALLAHPGVREAAVVAredgDGEKRLVAYVVP-----------------DEGGELDEEELRA-------HLAERL 416
                        570       580       590
                 ....*....|....*....|....*....|....
gi 544685990 544 PSFeqvkrftLLPQAFsTAMEEI--TPTLKLRRK 575
Cdd:cd05930  417 PDY-------MVPSAF-VVLDALplTPNGKVDRK 442
PRK06710 PRK06710
long-chain-fatty-acid--CoA ligase; Validated
18-544 1.77e-30

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 180666 [Multi-domain]  Cd Length: 563  Bit Score: 125.92  E-value: 1.77e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  18 KWLNRTALRFREQAQW----QEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYAT 93
Cdd:PRK06710  28 KYVEQMASRYPEKKALhflgKDITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNPL 107
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  94 NTAKQVEYIVNDADIKILFVGD-------------QEQFDQVYQIANNCP---QLMKIVAMKANMHLRDLPNACYWEDFL 157
Cdd:PRK06710 108 YTERELEYQLHDSGAKVILCLDlvfprvtnvqsatKIEHVIVTRIADFLPfpkNLLYPFVQKKQSNLVVKVSESETIHLW 187
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 158 NVVPNEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQ--LNAHDLALNVNEDDVSLSFLPLSHIFerawi 235
Cdd:PRK06710 188 NSVEKEVNTGVEVPCDPENDLALLQYTGGTTGFPKGVMLTHKNLVSNtlMGVQWLYNCKEGEEVVLGVLPFFHVY----- 262
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 236 ayvfhrGATNCYledtkhvrdALTTLKPTVMCAVPRFYEKIytaVWDKVEKaplHRRALFNWAirvgeqhyqieqPSQWL 315
Cdd:PRK06710 263 ------GMTAVM---------NLSIMQGYKMVLIPKFDMKM---VFEAIKK---HKVTLFPGA------------PTIYI 309
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 316 RL-QYALADKLVLTKLRALLGGrikmmpcgGAKLEASIGSFFHSI-GINIKLGYGMTETTATVSC---WQDKgfNPNSIG 390
Cdd:PRK06710 310 ALlNSPLLKEYDISSIRACISG--------SAPLPVEVQEKFETVtGGKLVEGYGLTESSPVTHSnflWEKR--VPGSIG 379
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 391 TLMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEETAkAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMK 459
Cdd:PRK06710 380 VPWPDTEAMImsletgealppGEIGEIVVKGPQIMKGYWNKPEETA-AVLQDGWLHTGDVGYMDEDGFFYVKDRKKDMIV 458
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 460 TSnGKYIAPQYIEGKIGKDKFIEQIAVIADAKKY----VSALIVPCFDSL--EEYAKQLNIKYQDRIELIKhsdiiqmfe 533
Cdd:PRK06710 459 AS-GFNVYPREVEEVLYEHEKVQEVVTIGVPDPYrgetVKAFVVLKEGTEcsEEELNQFARKYLAAYKVPK--------- 528
                        570
                 ....*....|.
gi 544685990 534 rrIHELQKELP 544
Cdd:PRK06710 529 --VYEFRDELP 537
AA-adenyl-dom TIGR01733
amino acid adenylation domain; This model represents a domain responsible for the specific ...
46-453 4.56e-30

amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.


Pssm-ID: 273779 [Multi-domain]  Cd Length: 409  Bit Score: 122.37  E-value: 4.56e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   46 DRFSYALIAQH-IDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQEQFDQVYQ 124
Cdd:TIGR01733  10 NRLARHLRAAGgVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTDSALASRLAGL 89
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  125 ianncpqlmkivamkanmhlrDLPNACYWEDFLNVVPNEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQ 204
Cdd:TIGR01733  90 ---------------------VLPVILLDPLELAALDDAPAPPPPDAPSGPDDLAYVIYTSGSTGRPKGVVVTHRSLVNL 148
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  205 LNAHDLALNVNEDDVSLSFLPLSH------IFeRAWIAyvfhrGATNCYLEDT------KHVRDALTTLKPTVMCAVPrf 272
Cdd:TIGR01733 149 LAWLARRYGLDPDDRVLQFASLSFdasveeIF-GALLA-----GATLVVPPEDeerddaALLAALIAEHPVTVLNLTP-- 220
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  273 yekiytAVWDKVEKAPLHRRALFNWAIRVGEqhyqieqpsqWLRLQyaladklVLTKLRAllggrikmmPCGGAKLeasi 352
Cdd:TIGR01733 221 ------SLLALLAAALPPALASLRLVILGGE----------ALTPA-------LVDRWRA---------RGPGARL---- 264
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  353 gsffhsigINiklGYGMTETTATVSCWQ-----DKGFNPNSIGTLMPNAE----------VKIGEENEILVRGGMVMRGY 417
Cdd:TIGR01733 265 --------IN---LYGPTETTVWSTATLvdpddAPRESPVPIGRPLANTRlyvldddlrpVPVGVVGELYIGGPGVARGY 333
                         410       420       430       440
                  ....*....|....*....|....*....|....*....|....
gi 544685990  418 YKKPEETAKAFTEDGFL--------RTGDVGEMDSCGNLFITDR 453
Cdd:TIGR01733 334 LNRPELTAERFVPDPFAggdgarlyRTGDLVRYLPDGNLEFLGR 377
PRK05605 PRK05605
long-chain-fatty-acid--CoA ligase; Validated
21-499 5.69e-30

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235531 [Multi-domain]  Cd Length: 573  Bit Score: 124.34  E-value: 5.69e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAV---PIYatnTAK 97
Cdd:PRK05605  47 DRPALDFFGA----TTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAVVVehnPLY---TAH 119
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  98 QVEYIVNDADIKILFVGDQEQfDQVYQIANNCPqLMKIV------AMKANMHL------------RD-----LPNACYWE 154
Cdd:PRK05605 120 ELEHPFEDHGARVAIVWDKVA-PTVERLRRTTP-LETIVsvnmiaAMPLLQRLalrlpipalrkaRAaltgpAPGTVPWE 197
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 155 DFLNVVPNEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANL---AHQLNAHDLALnVNEDDVSLSFLPLSHIFE 231
Cdd:PRK05605 198 TLVDAAIGGDGSDVSHPRPTPDDVALILYTSGTTGKPKGAQLTHRNLfanAAQGKAWVPGL-GDGPERVLAALPMFHAYG 276
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 232 RAW-IAYVFHRGATNCYLE--DTKHVRDALTTLKPTVMCAVPRFYEKIYTAvwdkvekaplhrralfnwairvgeqhyqi 308
Cdd:PRK05605 277 LTLcLTLAVSIGGELVLLPapDIDLILDAMKKHPPTWLPGVPPLYEKIAEA----------------------------- 327
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 309 eqpsqwlrlqyALADKLVLTKLRALLGGRIKMMPCGGAKLEASIGSFfhsiginIKLGYGMTETTATVSCwqdkgfNPNS 388
Cdd:PRK05605 328 -----------AEERGVDLSGVRNAFSGAMALPVSTVELWEKLTGGL-------LVEGYGLTETSPIIVG------NPMS 383
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 389 -------IGTLMPNAEVKI------------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLF 449
Cdd:PRK05605 384 ddrrpgyVGVPFPDTEVRIvdpedpdetmpdGEEGELLVRGPQVFKGYWNRPEETAKSF-LDGWFRTGDVVVMEEDGFIR 462
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....
gi 544685990 450 ITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVI----ADAKKYVSALIV 499
Cdd:PRK05605 463 IVDRIKELIITG-GFNVYPAEVEEVLREHPGVEDAAVVglprEDGSEEVVAAVV 515
PRK06178 PRK06178
acyl-CoA synthetase; Validated
31-492 8.64e-30

acyl-CoA synthetase; Validated


Pssm-ID: 235724 [Multi-domain]  Cd Length: 567  Bit Score: 124.00  E-value: 8.64e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  31 AQWQEMSwqtfqqeiDRFSyALIAQH-IDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIK 109
Cdd:PRK06178  62 AELDELS--------DRFA-ALLRQRgVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREHELSYELNDAGAE 132
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 110 ILFVGDQeQFDQVYQIANNCPQLMKIVAMKANM-----------HLRDLPNACY-WEDFLNVVPNEAEfEKRLNSKQLSD 177
Cdd:PRK06178 133 VLLALDQ-LAPVVEQVRAETSLRHVIVTSLADVlpaeptlplpdSLRAPRLAAAgAIDLLPALRACTA-PVPLPPPALDA 210
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 178 LFTLIYTSGTTGEPKGVMLDYANLAHQLNA-HDLALNVNEDDVSLSFLPlshIFeraWIA-------YVFHRGATNCYLe 249
Cdd:PRK06178 211 LAALNYTGGTTGMPKGCEHTQRDMVYTAAAaYAVAVVGGEDSVFLSFLP---EF---WIAgenfgllFPLFSGATLVLL- 283
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 dtkhVR-DALTtlkptVMCAVPRFYEKIYTAVWDK-VEKAPLHRRALFNwairvgeqhyqieqpsqwLR-LQYALADKLV 326
Cdd:PRK06178 284 ----ARwDAVA-----FMAAVERYRVTRTVMLVDNaVELMDHPRFAEYD------------------LSsLRQVRVVSFV 336
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 327 LtKLRALLGGRIKMMpCGGAKLEASigsffhsiginiklgYGMTET-TA-TVSC-WQDKGFN----PNSIGTLMPNAEVK 399
Cdd:PRK06178 337 K-KLNPDYRQRWRAL-TGSVLAEAA---------------WGMTEThTCdTFTAgFQDDDFDllsqPVFVGLPVPGTEFK 399
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 400 I-----------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAP 468
Cdd:PRK06178 400 IcdfetgellplGAEGEIVVRTPSLLKGYWNKPEATAEAL-RDGWLHTGDIGKIDEQGFLHYLGRRKEMLKV-NGMSVFP 477
                        490       500
                 ....*....|....*....|....*.
gi 544685990 469 QYIEGKIGKDKFIEQIAVI--ADAKK 492
Cdd:PRK06178 478 SEVEALLGQHPAVLGSAVVgrPDPDK 503
PRK06839 PRK06839
o-succinylbenzoate--CoA ligase;
34-487 8.99e-30

o-succinylbenzoate--CoA ligase;


Pssm-ID: 168698 [Multi-domain]  Cd Length: 496  Bit Score: 123.05  E-value: 8.99e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  34 QEMSWQTFQQEIDRFSYALIAQ-HIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILF 112
Cdd:PRK06839  26 EEMTYKQLHEYVSKVAAYLIYElNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLTENELIFQLKDSGTTVLF 105
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 113 VgdQEQFdqvyqiANNCPQLMKIVAMKANMHLRDlpnacywedflnvvPNEAEFEKRLN--SKQLSDLFTLIYTSGTTGE 190
Cdd:PRK06839 106 V--EKTF------QNMALSMQKVSYVQRVISITS--------------LKEIEDRKIDNfvEKNESASFIICYTSGTTGK 163
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 191 PKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLshiferawiayvFHRGATNCYledtkhvrdALTTLKPTVMCAVP 270
Cdd:PRK06839 164 PKGAVLTQENMFWNALNNTFAIDLTMHDRSIVLLPL------------FHIGGIGLF---------AFPTLFAGGVIIVP 222
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 RFYEKiytavwDKvekaplhrralfnwAIRVGEQH-----YQIEQPSQWLRLqyalADKLVLTKLRAllggrIKMMPCGG 345
Cdd:PRK06839 223 RKFEP------TK--------------ALSMIEKHkvtvvMGVPTIHQALIN----CSKFETTNLQS-----VRWFYNGG 273
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 346 AKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGF--NPNSIGTLMPNAEVKI----------GEENEILVRGGMV 413
Cdd:PRK06839 274 APCPEELMREFIDRGFLFGQGFGMTETSPTVFMLSEEDArrKVGSIGKPVLFCDYELidenknkvevGEVGELLIRGPNV 353
                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 544685990 414 MRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:PRK06839 354 MKEYWNRPDATEETI-QDGWLCTGDLARVDEDGFVYIVGRKKE-MIISGGENIYPLEVEQVINKLSDVYEVAVV 425
PRK05677 PRK05677
long-chain-fatty-acid--CoA ligase; Validated
44-472 9.83e-30

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 168170 [Multi-domain]  Cd Length: 562  Bit Score: 123.72  E-value: 9.83e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  44 EIDRFSYALIA---QHIDIQ--DKIGIFANNMPRWTIADFGAMQARAVAV---PIYatnTAKQVEYIVNDADIKILF--- 112
Cdd:PRK05677  54 ELYKLSGAFAAwlqQHTDLKpgDRIAVQLPNVLQYPVAVFGAMRAGLIVVntnPLY---TAREMEHQFNDSGAKALVcla 130
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 113 --------VGDQEQFDQVY--QIANNCPQLMKIVAMKANMHLR------DLPNACyweDFLNVVPNEAEFEKRLNSKQLS 176
Cdd:PRK05677 131 nmahlaekVLPKTGVKHVIvtEVADMLPPLKRLLINAVVKHVKkmvpayHLPQAV---KFNDALAKGAGQPVTEANPQAD 207
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANL-AHQLNAHDL-ALNVNED-DVSLSFLPLSHIFerawiAYVFHRGATncyLEDTKH 253
Cdd:PRK05677 208 DVAVLQYTGGTTGVAKGAMLTHRNLvANMLQCRALmGSNLNEGcEILIAPLPLYHIY-----AFTFHCMAM---MLIGNH 279
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 254 vrdalttlkpTVMCAVPRfyekiytavwdkveKAPLHRRALFNWAIR--VGEQhyqieqpsqwlRLQYALADKlvlTKLR 331
Cdd:PRK05677 280 ----------NILISNPR--------------DLPAMVKELGKWKFSgfVGLN-----------TLFVALCNN---EAFR 321
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 332 ALLGGRIKMMPCGGAKLEASIGSFFHSI-GINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI---------- 400
Cdd:PRK05677 322 KLDFSALKLTLSGGMALQLATAERWKEVtGCAICEGYGMTETSPVVSVNPSQAIQVGTIGIPVPSTLCKVidddgnelpl 401
                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 544685990 401 GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIE 472
Cdd:PRK05677 402 GEVGELCVKGPQVMKGYWQRPEATDEILDSDGWLKTGDIALIQEDGYMRIVDRKKDMILVS-GFNVYPNELE 472
FCS cd05921
Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl ...
22-591 1.62e-29

Feruloyl-CoA synthetase (FCS); Feruloyl-CoA synthetase is an essential enzyme in the feruloyl acid degradation pathway and enables some proteobacteria to grow on media containing feruloyl acid as the sole carbon source. It catalyzes the transfer of CoA to the carboxyl group of ferulic acid, which then forms feruloyl-CoA in the presence of ATP and Mg2. The resulting feruloyl-CoA is further degraded to vanillin and acetyl-CoA. Feruloyl-CoA synthetase (FCS) is a subfamily of the adenylate-forming enzymes superfamily.


Pssm-ID: 341245 [Multi-domain]  Cd Length: 561  Bit Score: 122.93  E-value: 1.62e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  22 RTALRFREQAQ-WQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI---YATNTAK 97
Cdd:cd05921   11 RTWLAEREGNGgWRRVTYAEALRQVRAIAQGLLDLGLSAERPLLILSGNSIEHALMALAAMYAGVPAAPVspaYSLMSQD 90
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  98 --QVEYIVNDADIKILFVGDQEQFDQVyqIANNCPQLMKIVamkanmHLRDLPNACYWEDFLNVV--PNEAEFEKRLNSK 173
Cdd:cd05921   91 laKLKHLFELLKPGLVFAQDAAPFARA--LAAIFPLGTPLV------VSRNAVAGRGAISFAELAatPPTAAVDAAFAAV 162
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 174 QLSDLFTLIYTSGTTGEPKGVMLDYANLA--HQLNAHDLALNVNEDDVSLSFLPLSHIF-ERAWIAYVFHRGATnCYLED 250
Cdd:cd05921  163 GPDTVAKFLFTSGSTGLPKAVINTQRMLCanQAMLEQTYPFFGEEPPVLVDWLPWNHTFgGNHNFNLVLYNGGT-LYIDD 241
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 251 TKHVRDAL-TTLK------PTVMCAVPRFYEKIYTAVwdkvEKAPLHRRALFNwaiRVGEQHYQIEQPSQ--WLRLQyAL 321
Cdd:cd05921  242 GKPMPGGFeETLRnlreisPTVYFNVPAGWEMLVAAL----EKDEALRRRFFK---RLKLMFYAGAGLSQdvWDRLQ-AL 313
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 322 ADKLVltklrallGGRIKMMPcggakleasigsffhsiginiklGYGMTET--TATVSCW-QDKgfnPNSIGTLMPNAEV 398
Cdd:cd05921  314 AVATV--------GERIPMMA-----------------------GLGATETapTATFTHWpTER---SGLIGLPAPGTEL 359
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 399 KI---GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEM----DSCGNLFITDRLKELMKTSNGKYIA--PQ 469
Cdd:cd05921  360 KLvpsGGKYEVRVKGPNVTPGYWRQPELTAQAFDEEGFYCLGDAAKLadpdDPAKGLVFDGRVAEDFKLASGTWVSvgPL 439
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 470 YIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEEYAKqlnIKYQDRIELIKHSDIIQMFERRIHELQKEL-PSFEQ 548
Cdd:cd05921  440 RARAVAACAPLVHDAVVAGEDRAEVGALVFPDLLACRRLVG---LQEASDAEVLRHAKVRAAFRDRLAALNGEAtGSSSR 516
                        570       580       590       600
                 ....*....|....*....|....*....|....*....|...
gi 544685990 549 VKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMYNE 591
Cdd:cd05921  517 IARALLLDEPPSIDKGEITDKGYINQRAVLERRAALVERLYAD 559
PRK08315 PRK08315
AMP-binding domain protein; Validated
12-500 2.17e-29

AMP-binding domain protein; Validated


Pssm-ID: 236236 [Multi-domain]  Cd Length: 559  Bit Score: 122.61  E-value: 2.17e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  12 FRLQAKKWLNRTALRFREQ-AQWqemSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI 90
Cdd:PRK08315  22 LDRTAARYPDREALVYRDQgLRW---TYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFATAKIGAILVTI 98
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  91 yatNTA---KQVEYIVNDADIKILFVGDQEQ----FDQVYQIANNC-------------PQLMKIVAMKANMHlRDLPNa 150
Cdd:PRK08315  99 ---NPAyrlSELEYALNQSGCKALIAADGFKdsdyVAMLYELAPELatcepgqlqsarlPELRRVIFLGDEKH-PGMLN- 173
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 151 cyWEDFLN--VVPNEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHqlNAHDLALNVN---EDDVSLSfLP 225
Cdd:PRK08315 174 --FDELLAlgRAVDDAELAARQATLDPDDPINIQYTSGTTGFPKGATLTHRNILN--NGYFIGEAMKlteEDRLCIP-VP 248
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 226 LSHIFER--AWIAYVFHrGATNCYLEDtkhVRDALTTL------KPTVMCAVPrfyeKIYTAVWDkvekapLHRRALFNw 297
Cdd:PRK08315 249 LYHCFGMvlGNLACVTH-GATMVYPGE---GFDPLATLaaveeeRCTALYGVP----TMFIAELD------HPDFARFD- 313
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 298 airvgeqhyqieqpsqwlrlqyaladklvLTKLRallGGrikMM---PCGGAKLEASIGSFfHSIGINIklGYGMTETtA 374
Cdd:PRK08315 314 -----------------------------LSSLR---TG---IMagsPCPIEVMKRVIDKM-HMSEVTI--AYGMTET-S 354
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 375 TVSCwQDKGFNP-----NSIGTLMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGD 438
Cdd:PRK08315 355 PVST-QTRTDDPlekrvTTVGRALPHLEVKIvdpetgetvprGEQGELCTRGYSVMKGYWNDPEKTAEAIDADGWMHTGD 433
                        490       500       510       520       530       540       550
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 544685990 439 VGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEgkigkdKFIEQIAVIADA-------KKY---VSALIVP 500
Cdd:PRK08315 434 LAVMDEEGYVNIVGRIKD-MIIRGGENIYPREIE------EFLYTHPKIQDVqvvgvpdEKYgeeVCAWIIL 498
OSB_CoA_lg cd05912
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ...
156-491 2.73e-29

O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.


Pssm-ID: 341238 [Multi-domain]  Cd Length: 411  Bit Score: 120.14  E-value: 2.73e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 156 FLN--VVPNEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANlaHQLNAHDLALN--VNEDDVSLSFLPLSHI-- 229
Cdd:cd05912   55 LLNtrLTPNELAFQLKDSDVKLDDIATIMYTSGTTGKPKGVQQTFGN--HWWSAIGSALNlgLTEDDNWLCALPLFHIsg 132
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 230 ---FERAWIAyvfhrgATNCYLE---DTKHVRDALTTLKPTVMCAVPrfyekiytavwdkvekaplhrrALFNWAIRVGE 303
Cdd:cd05912  133 lsiLMRSVIY------GMTVYLVdkfDAEQVLHLINSGKVTIISVVP----------------------TMLQRLLEILG 184
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 304 QHYqieqPSQwLRLqyaladklvltklrALLGGRikmmPCGGAKLEASigsffHSIGINIKLGYGMTETtatvsCWQDKG 383
Cdd:cd05912  185 EGY----PNN-LRC--------------ILLGGG----PAPKPLLEQC-----KEKGIPVYQSYGMTET-----CSQIVT 231
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 384 FNP-------NSIGTLMPNAEVKI-------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLF 449
Cdd:cd05912  232 LSPedalnkiGSAGKPLFPVELKIeddgqppYEVGEILLKGPNVTKGYLNRPDATEESF-ENGWFKTGDIGYLDEEGFLY 310
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....
gi 544685990 450 ITDRLKELMkTSNGKYIAPQYIEGKIGKDKFIEQIAVIA--DAK 491
Cdd:cd05912  311 VLDRRSDLI-ISGGENIYPAEIEEVLLSHPAIKEAGVVGipDDK 353
FADD10 cd17635
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ...
176-493 3.43e-29

adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.


Pssm-ID: 341290 [Multi-domain]  Cd Length: 340  Bit Score: 118.52  E-value: 3.43e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 176 SDLFTLIYTSGTTGEPKGVMLDYANL-AHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAY-VFHRGA--TNCYLEDT 251
Cdd:cd17635    1 EDPLAVIFTSGTTGEPKAVLLANKTFfAVPDILQKEGLNWVVGDVTYLPLPATHIGGLWWILTcLIHGGLcvTGGENTTY 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 252 KHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEKAPlhrralfnwairvgeqhyqieqpsqwlrlqyaladklvltKLR 331
Cdd:cd17635   81 KSLFKILTTNAVTTTCLVPTLLSKLVSELKSANATVP----------------------------------------SLR 120
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 332 ALLGGrikmmpcGGAKLEASIGSFFHSIGINIKLGYGMTETTaTVSCWQ--DKGFNPNSIGTLMPNAEVKI--------- 400
Cdd:cd17635  121 LIGYG-------GSRAIAADVRFIEATGLTNTAQVYGLSETG-TALCLPtdDDSIEINAVGRPYPGVDVYLaatdgiagp 192
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -GEENEILVRGGMVMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEgkigkdK 479
Cdd:cd17635  193 sASFGTIWIKSPANMLGYWNNPERTAEVLI-DGWVNTGDLGERREDGFLFITGRSSESI-NCGGVKIAPDEVE------R 264
                        330
                 ....*....|....
gi 544685990 480 FIEQIAVIADAKKY 493
Cdd:cd17635  265 IAEGVSGVQECACY 278
OSB_MenE-like cd17630
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ...
177-581 3.83e-29

O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.


Pssm-ID: 341285 [Multi-domain]  Cd Length: 325  Bit Score: 118.20  E-value: 3.83e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIferAWIAYVfhrgatncyledtkhVRD 256
Cdd:cd17630    1 RLATVILTSGSTGTPKAVVHTAANLLASAAGLHSRLGFGGGDSWLLSLPLYHV---GGLAIL---------------VRS 62
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 257 ALTTLKPTVmcavprfyekiytavwdkvekaPLHRRALFNWAIRVGEQHYQIeQPSQwlrLQYALADKLV---LTKLRAL 333
Cdd:cd17630   63 LLAGAELVL----------------------LERNQALAEDLAPPGVTHVSL-VPTQ---LQRLLDSGQGpaaLKSLRAV 116
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 334 LggrikmmpCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKIGEENEILVRGGMV 413
Cdd:cd17630  117 L--------LGGAPIPPELLERAADRGIPLYTTYGMTETASQVATKRPDGFGRGGVGVLLPGRELRIVEDGEIWVGGASL 188
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 414 MRGYYKKPEEtaKAFTEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEgkigkdkfieqiAVIADAKKY 493
Cdd:cd17630  189 AMGYLRGQLV--PEFNEDGWFTTKDLGELHADGRLTVLGRADN-MIISGGENIQPEEIE------------AALAAHPAV 253
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 494 VSALIVPCFDslEEYAKQLNIKYQDRIELIkHSDIIQMferriheLQKELPSFEQVKRFTLLPQAfstameEITPTLKLR 573
Cdd:cd17630  254 RDAFVVGVPD--EELGQRPVAVIVGRGPAD-PAELRAW-------LKDKLARFKLPKRIYPVPEL------PRTGGGKVD 317

                 ....*...
gi 544685990 574 RKVIMQRY 581
Cdd:cd17630  318 RRALRAWL 325
ttLC_FACS_AEE21_like cd12118
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ...
37-488 4.44e-29

Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.


Pssm-ID: 341283 [Multi-domain]  Cd Length: 486  Bit Score: 120.87  E-value: 4.44e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  37 SWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQ 116
Cdd:cd12118   31 TWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNALNTRLDAEEIAFILRHSEAKVLFVDRE 110
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 117 EQFDQVYQIANncpqlmkivamkanmhlrdlpnacywEDFLNVVPNEaEFekrlnskqlsDLFTLIYTSGTTGEPKGVMl 196
Cdd:cd12118  111 FEYEDLLAEGD--------------------------PDFEWIPPAD-EW----------DPIALNYTSGTTGRPKGVV- 152
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 197 dYANLAHQLNAHDLAL--NVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLE--DTKHVRDALTTLKPTVMCAVPrf 272
Cdd:cd12118  153 -YHHRGAYLNALANILewEMKQHPVYLWTLPMFHCNGWCFPWTVAAVGGTNVCLRkvDAKAIYDLIEKHKVTHFCGAP-- 229
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 273 yekiytAVWDKVEKAPLHRRALFNWAIRVgeqhyqieqpsqwlrlqyaladklvLTklrallggrikmmpcGGAKLEASI 352
Cdd:cd12118  230 ------TVLNMLANAPPSDARPLPHRVHV-------------------------MT---------------AGAPPPAAV 263
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 353 GSFFHSIGINIKLGYGMTETT--ATVSCWQDK---------------------------GFNPNSIGTLmPNAEVKIGEe 403
Cdd:cd12118  264 LAKMEELGFDVTHVYGLTETYgpATVCAWKPEwdelpteerarlkarqgvryvgleevdVLDPETMKPV-PRDGKTIGE- 341
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 404 neILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEGKIGKDKFIEQ 483
Cdd:cd12118  342 --IVFRGNIVMKGYLKNPEATAEAF-RGGWFHSGDLAVIHPDGYIEIKDRSKDII-ISGGENISSVEVEGVLYKHPAVLE 417

                 ....*
gi 544685990 484 IAVIA 488
Cdd:cd12118  418 AAVVA 422
PRK08316 PRK08316
acyl-CoA synthetase; Validated
12-500 1.60e-28

acyl-CoA synthetase; Validated


Pssm-ID: 181381 [Multi-domain]  Cd Length: 523  Bit Score: 119.65  E-value: 1.60e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  12 FRLQAKKWLNRTALRFREQAqWqemSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:PRK08316  17 LRRSARRYPDKTALVFGDRS-W---TYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVN 92
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  92 ATNTAKQVEYIVNDADIKILFVGDQeqfdqVYQIANNCPQLMKIVAMKANMHLRDLPNACYWEDFLNVVPNE--AEFEKR 169
Cdd:PRK08316  93 FMLTGEELAYILDHSGARAFLVDPA-----LAPTAEAALALLPVDTLILSLVLGGREAPGGWLDFADWAEAGsvAEPDVE 167
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 170 LNSkqlSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSH-----IFERAWIaYVfhrGAT 244
Cdd:PRK08316 168 LAD---DDLAQILYTSGTESLPKGAMLTHRALIAEYVSCIVAGDMSADDIPLHALPLYHcaqldVFLGPYL-YV---GAT 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 245 NCYLE--DTKHVRDALTTLKPTVMCAVPrfyekiytAVWdkvekAPLHRRALFnwairvgeqhyqieqpsqwlrlqyala 322
Cdd:PRK08316 241 NVILDapDPELILRTIEAERITSFFAPP--------TVW-----ISLLRHPDF--------------------------- 280
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 323 DKLVLTKLR-ALLGGRIkmMPcgGAKLEaSIGSFFHSIGI-NIklgYGMTET--TATVSCWQDKGFNPNSIGTLMPNAEV 398
Cdd:PRK08316 281 DTRDLSSLRkGYYGASI--MP--VEVLK-ELRERLPGLRFyNC---YGQTEIapLATVLGPEEHLRRPGSAGRPVLNVET 352
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 399 KI----------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAP 468
Cdd:PRK08316 353 RVvdddgndvapGEVGEIVHRSPQLMLGYWDDPEKTAEAF-RGGWFHSGDLGVMDEEGYITVVDRKKDMIKTG-GENVAS 430
                        490       500       510
                 ....*....|....*....|....*....|....*.
gi 544685990 469 QYIEGKIGKDKFIEQIAVIA--DAK--KYVSALIVP 500
Cdd:PRK08316 431 REVEEALYTHPAVAEVAVIGlpDPKwiEAVTAVVVP 466
FACL_like_2 cd05917
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
183-487 3.07e-28

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341241 [Multi-domain]  Cd Length: 349  Bit Score: 115.84  E-value: 3.07e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 183 YTSGTTGEPKGVMLDYANLAHqlNAHDLA--LNVNEDDVSLSFLPLSHIFER--AWIAYVFHrGATNCYLEDTKHVRDAL 258
Cdd:cd05917    9 FTSGTTGSPKGATLTHHNIVN--NGYFIGerLGLTEQDRLCIPVPLFHCFGSvlGVLACLTH-GATMVFPSPSFDPLAVL 85
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 259 TTL---KPTVMCAVPRFYEKIytavwdkvekaplhrralfnwairvgeqhyqIEQPSQwlrlqyalaDKLVLTKLRallG 335
Cdd:cd05917   86 EAIekeKCTALHGVPTMFIAE-------------------------------LEHPDF---------DKFDLSSLR---T 122
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 336 GRIKMMPCGGAKLEASIgSFFHSIGINIklGYGMTETTATvsCWQDKGFNP-----NSIGTLMPNAEVKI---------- 400
Cdd:cd05917  123 GIMAGAPCPPELMKRVI-EVMNMKDVTI--AYGMTETSPV--STQTRTDDSiekrvNTVGRIMPHTEAKIvdpeggivpp 197
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDK 479
Cdd:cd05917  198 vGVPGELCIRGYSVMKGYWNDPEKTAEAIDGDGWLHTGDLAVMDEDGYCRIVGRIKD-MIIRGGENIYPREIEEFLHTHP 276

                 ....*...
gi 544685990 480 FIEQIAVI 487
Cdd:cd05917  277 KVSDVQVV 284
PRK08633 PRK08633
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
172-454 6.27e-28

2-acyl-glycerophospho-ethanolamine acyltransferase; Validated


Pssm-ID: 236315 [Multi-domain]  Cd Length: 1146  Bit Score: 119.64  E-value: 6.27e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  172 SKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFE---RAW------IAYVFHRG 242
Cdd:PRK08633  778 TFKPDDTATIIFSSGSEGEPKGVMLSHHNILSNIEQISDVFNLRNDDVILSSLPFFHSFGltvTLWlpllegIKVVYHPD 857
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  243 ATncyleDTKHVRDALTTLKPTVMCAVPRFYeKIYTavwdKVEKAplhrralfnwairvgeqhyqieqpsqwlrlqyala 322
Cdd:PRK08633  858 PT-----DALGIAKLVAKHRATILLGTPTFL-RLYL----RNKKL----------------------------------- 892
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  323 DKLVLTKLRallggrikMMPCGGAKLEASIG-SFFHSIGINIKLGYGMTETTATVSC----------WQDKGFNPNSIGT 391
Cdd:PRK08633  893 HPLMFASLR--------LVVAGAEKLKPEVAdAFEEKFGIRILEGYGATETSPVASVnlpdvlaadfKRQTGSKEGSVGM 964
                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990  392 LMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLR---TGDVGEMDSCGNLFITDRL 454
Cdd:PRK08633  965 PLPGVAVRIvdpetfeelppGEDGLILIGGPQVMKGYLGDPEKTAEVIKDIDGIGwyvTGDKGHLDEDGFLTITDRY 1041
caiC PRK08008
putative crotonobetaine/carnitine-CoA ligase; Validated
16-487 9.31e-28

putative crotonobetaine/carnitine-CoA ligase; Validated


Pssm-ID: 181195 [Multi-domain]  Cd Length: 517  Bit Score: 117.09  E-value: 9.31e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  16 AKKWLNRTALRFREQA-QWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATN 94
Cdd:PRK08008  17 ADVYGHKTALIFESSGgVVRRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARL 96
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  95 TAKQVEYIVNDADIKILFVgdQEQFDQVY-QIANNCPQLMKIVAMkANMHLRDLPNACYWEDFLNVVPNEAEFEKRLNSk 173
Cdd:PRK08008  97 LREESAWILQNSQASLLVT--SAQFYPMYrQIQQEDATPLRHICL-TRVALPADDGVSSFTQLKAQQPATLCYAPPLST- 172
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 174 qlSDLFTLIYTSGTTGEPKGVMLDYANL--AHQLNAHDLALnvNEDDVSLSFLPLSHI-FERAWIAYVFHRGATNCYLED 250
Cdd:PRK08008 173 --DDTAEILFTSGTTSRPKGVVITHYNLrfAGYYSAWQCAL--RDDDVYLTVMPAFHIdCQCTAAMAAFSAGATFVLLEK 248
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 251 tkhvrdalttlkptvmcavprfyekiYTA--VWDKVEKaplhRRALFNWAIRVGEQHYQIEQPSQWLRlQYALADKLVLt 328
Cdd:PRK08008 249 --------------------------YSAraFWGQVCK----YRATITECIPMMIRTLMVQPPSANDR-QHCLREVMFY- 296
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 klrallggrikmMPCGGAKLEASIGSFfhsiGINIKLGYGMTETtaTVSCWQDKGFNPN---SIGTLMPNAEVKIGEEN- 404
Cdd:PRK08008 297 ------------LNLSDQEKDAFEERF----GVRLLTSYGMTET--IVGIIGDRPGDKRrwpSIGRPGFCYEAEIRDDHn 358
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 ---------EILVR---GGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIE 472
Cdd:PRK08008 359 rplpageigEICIKgvpGKTIFKEYYLDPKATAKVLEADGWLHTGDTGYVDEEGFFYFVDRRCNMIKRG-GENVSCVELE 437
                        490
                 ....*....|....*
gi 544685990 473 GKIGKDKFIEQIAVI 487
Cdd:PRK08008 438 NIIATHPKIQDIVVV 452
FadD3 cd17638
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ...
177-487 1.01e-27

acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.


Pssm-ID: 341293 [Multi-domain]  Cd Length: 330  Bit Score: 114.14  E-value: 1.01e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIF--ERAWIAYVFhRGATNCYLE--DTK 252
Cdd:cd17638    1 DVSDIMFTSGTTGRSKGVMCAHRQTLRAAAAWADCADLTEDDRYLIINPFFHTFgyKAGIVACLL-TGATVVPVAvfDVD 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 253 HVRDALTTLKPTVMCAVPRFYEKIytavwdkvekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADKLVLTKLRA 332
Cdd:cd17638   80 AILEAIERERITVLPGPPTLFQSL----------------------------------------LDHPGRKKFDLSSLRA 119
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 333 LLggrikmmpCGGAKLEASIGSFFHS-IGI-NIKLGYGMTETTATVSCWQDKGFN--PNSIGTLMPNAEVKIGEENEILV 408
Cdd:cd17638  120 AV--------TGAATVPVELVRRMRSeLGFeTVLTAYGLTEAGVATMCRPGDDAEtvATTCGRACPGFEVRIADDGEVLV 191
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 544685990 409 RGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:cd17638  192 RGYNVMQGYLDDPEATAEAIDADGWLHTGDVGELDERGYLRITDRLKD-MYIVGGFNVYPAEVEGALAEHPGVAQVAVI 269
PRK03640 PRK03640
o-succinylbenzoate--CoA ligase;
21-491 1.45e-27

o-succinylbenzoate--CoA ligase;


Pssm-ID: 235146 [Multi-domain]  Cd Length: 483  Bit Score: 116.22  E-value: 1.45e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:PRK03640  17 DRTAIEFEEK----KVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAVAVLLNTRLSREELL 92
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFVGD--QEQFDQVYQIanncpqlmkivamkanmhlrdlpnacYWEDFLNVVPNEAEFEkrlNSKQLSDL 178
Cdd:PRK03640  93 WQLDDAEVKCLITDDdfEAKLIPGISV--------------------------KFAELMNGPKEEAEIQ---EEFDLDEV 143
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 179 FTLIYTSGTTGEPKGVMLDYANlaHQLNAHDLALN--VNEDDVSLSFLPLSHI-----FERAWIaYvfhrGATnCYLE-- 249
Cdd:PRK03640 144 ATIMYTSGTTGKPKGVIQTYGN--HWWSAVGSALNlgLTEDDCWLAAVPIFHIsglsiLMRSVI-Y----GMR-VVLVek 215
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 -DTKHVRDALTTLKPTVMCAVPRFYEKIytavwdkvekaplhrralfnwAIRVGEQHYqieqPSqwlrlqyaladklvlt 328
Cdd:PRK03640 216 fDAEKINKLLQTGGVTIISVVSTMLQRL---------------------LERLGEGTY----PS---------------- 254
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 KLRA-LLGGRikmmPCGGAKLEASigsffHSIGINIKLGYGMTETTATVsCWQDKGFNPNSIGT----LMPnAEVKI--- 400
Cdd:PRK03640 255 SFRCmLLGGG----PAPKPLLEQC-----KEKGIPVYQSYGMTETASQI-VTLSPEDALTKLGSagkpLFP-CELKIekd 323
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 ------GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEGK 474
Cdd:PRK03640 324 gvvvppFEEGEIVVKGPNVTKGYLNREDATRETF-QDGWFKTGDIGYLDEEGFLYVLDRRSDLI-ISGGENIYPAEIEEV 401
                        490
                 ....*....|....*....
gi 544685990 475 IGKDKFIEQIAVI--ADAK 491
Cdd:PRK03640 402 LLSHPGVAEAGVVgvPDDK 420
PRK08751 PRK08751
long-chain fatty acid--CoA ligase;
9-544 1.12e-26

long-chain fatty acid--CoA ligase;


Pssm-ID: 181546 [Multi-domain]  Cd Length: 560  Bit Score: 114.20  E-value: 1.12e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   9 VNRFRLQAKKWLNRTAlRFREQAQW----QEMSWQTFQQEIDRF-SYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQA 83
Cdd:PRK08751  21 LEQFRTVAEVFATSVA-KFADRPAYhsfgKTITYREADQLVEQFaAYLLGELQLKKGDRVALMMPNCLQYPIATFGVLRA 99
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  84 RAVAVPIYATNTAKQVEYIVNDADIKILFVGDQeqF-DQVYQIANNCPqlMKIVAMKANMHLRDLPNACYWEDFLNVV-- 160
Cdd:PRK08751 100 GLTVVNVNPLYTPRELKHQLIDSGASVLVVIDN--FgTTVQQVIADTP--VKQVITTGLGDMLGFPKAALVNFVVKYVkk 175
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 161 -------PNEAEFEK--------RLNSKQLS--DLFTLIYTSGTTGEPKGVMLDYANL-AHQLNAHDLALNVNE----DD 218
Cdd:PRK08751 176 lvpeyriNGAIRFREalalgrkhSMPTLQIEpdDIAFLQYTGGTTGVAKGAMLTHRNLvANMQQAHQWLAGTGKleegCE 255
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 219 VSLSFLPLSHIFERAWIAYVFHR-GATNCYLEDTKHVRDALTTLKPTvmcavpRFyeKIYTAVwdkvekaplhrRALFNW 297
Cdd:PRK08751 256 VVITALPLYHIFALTANGLVFMKiGGCNHLISNPRDMPGFVKELKKT------RF--TAFTGV-----------NTLFNG 316
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 298 AirvgeqhyqieqpsqwlrLQYALADKLVLTKLRALLGGrikmmpcgGAKLEASIGSFFHSI-GINIKLGYGMTETT--A 374
Cdd:PRK08751 317 L------------------LNTPGFDQIDFSSLKMTLGG--------GMAVQRSVAERWKQVtGLTLVEAYGLTETSpaA 370
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 375 TVSCWQDKGFNpNSIGTLMPNAEV----------KIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDS 444
Cdd:PRK08751 371 CINPLTLKEYN-GSIGLPIPSTDAcikddagtvlAIGEIGELCIKGPQVMKGYWKRPEETAKVMDADGWLHTGDIARMDE 449
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 445 CGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVIA--DAK--KYVSALIV---PCFDSLEeyakqlnIKYQ 517
Cdd:PRK08751 450 QGFVYIVDRKKDMILVS-GFNVYPNEIEDVIAMMPGVLEVAAVGvpDEKsgEIVKVVIVkkdPALTAED-------VKAH 521
                        570       580
                 ....*....|....*....|....*..
gi 544685990 518 DRIELIKHSdiiqmfERRIHELQKELP 544
Cdd:PRK08751 522 ARANLTGYK------QPRIIEFRKELP 542
PRK12582 PRK12582
acyl-CoA synthetase; Provisional
17-591 1.70e-26

acyl-CoA synthetase; Provisional


Pssm-ID: 237144 [Multi-domain]  Cd Length: 624  Bit Score: 114.37  E-value: 1.70e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  17 KKWLNRtalRFREQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI---YA- 92
Cdd:PRK12582  65 RPWLAQ---REPGHGQWRKVTYGEAKRAVDALAQALLDLGLDPGRPVMILSGNSIEHALMTLAAMQAGVPAAPVspaYSl 141
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  93 --TNTAKqVEYIVNDADIKILFVGDQEQFDQVYQIAN-NCPQLMKIVAM---KANMHLRDLPnacywedflnVVPNEAEF 166
Cdd:PRK12582 142 msHDHAK-LKHLFDLVKPRVVFAQSGAPFARALAALDlLDVTVVHVTGPgegIASIAFADLA----------ATPPTAAV 210
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 167 EKRLNSKQLSDLFTLIYTSGTTGEPKGV-----MLdYANLAHQLNAHDLALNvNEDDVSLSFLPLSHIFE-RAWIAYVFH 240
Cdd:PRK12582 211 AAAIAAITPDTVAKYLFTSGSTGMPKAVintqrMM-CANIAMQEQLRPREPD-PPPPVSLDWMPWNHTMGgNANFNGLLW 288
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 241 RGATnCYLEDTKHVRD-------ALTTLKPTVMCAVPrfyeKIYTAVWDKVEKAPLHRRALFNwaiRVGEQHYQIEQPSQ 313
Cdd:PRK12582 289 GGGT-LYIDDGKPLPGmfeetirNLREISPTVYGNVP----AGYAMLAEAMEKDDALRRSFFK---NLRLMAYGGATLSD 360
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 314 --WLRLQyALADKLVltklrallGGRIKMMPcggakleasigsffhsiginiklGYGMTET--TATVSCWQDKgfNPNSI 389
Cdd:PRK12582 361 dlYERMQ-ALAVRTT--------GHRIPFYT-----------------------GYGATETapTTTGTHWDTE--RVGLI 406
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 390 GTLMPNAEVK---IGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVG----EMDSCGNLFITDRLKELMKTSN 462
Cdd:PRK12582 407 GLPLPGVELKlapVGDKYEVRVKGPNVTPGYHKDPELTAAAFDEEGFYRLGDAArfvdPDDPEKGLIFDGRVAEDFKLST 486
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 463 GKYIAPqyieGKIGKDKFIEQIAVIADA------KKYVSALIVPCFDSLEEYAKQLNIKYQDrieLIKHSDIIQMFERRI 536
Cdd:PRK12582 487 GTWVSV----GTLRPDAVAACSPVIHDAvvagqdRAFIGLLAWPNPAACRQLAGDPDAAPED---VVKHPAVLAILREGL 559
                        570       580       590       600       610
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 537 HELQKELP-SFEQVKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMYNE 591
Cdd:PRK12582 560 SAHNAEAGgSSSRIARALLMTEPPSIDAGEITDKGYINQRAVLERRAALVERLYAE 615
PLN02246 PLN02246
4-coumarate--CoA ligase
177-472 1.84e-26

4-coumarate--CoA ligase


Pssm-ID: 215137 [Multi-domain]  Cd Length: 537  Bit Score: 113.54  E-value: 1.84e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANL----AHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHR-GATNCYLE-- 249
Cdd:PLN02246 180 DVVALPYSSGTTGLPKGVMLTHKGLvtsvAQQVDGENPNLYFHSDDVILCVLPMFHIYSLNSVLLCGLRvGAAILIMPkf 259
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 DTKHVRDALTTLKPTVMCAVPrfyeKIYTAVwdkvEKAPLhrralfnwairvgeqhyqieqpsqwlrlqyalADKLVLTK 329
Cdd:PLN02246 260 EIGALLELIQRHKVTIAPFVP----PIVLAI----AKSPV--------------------------------VEKYDLSS 299
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 330 LRALLGGRIKMmpcgGAKLEASIGSFFHsigiNIKL--GYGMTETTATVS-CWqdkGF-------NPNSIGTLMPNAEVK 399
Cdd:PLN02246 300 IRMVLSGAAPL----GKELEDAFRAKLP----NAVLgqGYGMTEAGPVLAmCL---AFakepfpvKSGSCGTVVRNAELK 368
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 400 I-----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAP 468
Cdd:PLN02246 369 IvdpetgaslprNQPGEICIRGPQIMKGYLNDPEATANTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKY-KGFQVAP 447

                 ....
gi 544685990 469 QYIE 472
Cdd:PLN02246 448 AELE 451
FAAL cd05931
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ...
21-472 3.35e-26

Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.


Pssm-ID: 341254 [Multi-domain]  Cd Length: 547  Bit Score: 112.72  E-value: 3.35e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  21 NRTALRF--REQAQWQEMSWQTFQQEIDRFSYALiaQHIDIQ-DKIGIFANNMPRWTIADFGAMQARAVAVPIYATN--- 94
Cdd:cd05931    8 DRPAYTFldDEGGREETLTYAELDRRARAIAARL--QAVGKPgDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLPPPTpgr 85
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  95 TAKQVEYIVNDADIKILFVgDQEQFDQVYQIANNCPQlmkivamkanmhlrDLPNACYWEDFLNVVPNEAEfekRLNSKQ 174
Cdd:cd05931   86 HAERLAAILADAGPRVVLT-TAAALAAVRAFAASRPA--------------AGTPRLLVVDLLPDTSAADW---PPPSPD 147
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 175 LSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSH----IFerAWIAYVFHrGATNCYLED 250
Cdd:cd05931  148 PDDIAYLQYTSGSTGTPKGVVVTHRNLLANVRQIRRAYGLDPGDVVVSWLPLYHdmglIG--GLLTPLYS-GGPSVLMSP 224
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 251 TKHVRD------ALTTLKPTVMcAVPRFyekiytavwdkvekaplhrrAlFNWAIRvgeqhyqieqpsqwlRLQYALADK 324
Cdd:cd05931  225 AAFLRRplrwlrLISRYRATIS-AAPNF--------------------A-YDLCVR---------------RVRDEDLEG 267
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 325 LVLTKLRALLGG--RI----------KMMPCGgAKLEAsigsffhsiginIKLGYGMTETTATVSC-WQDKG-----FNP 386
Cdd:cd05931  268 LDLSSWRVALNGaePVrpatlrrfaeAFAPFG-FRPEA------------FRPSYGLAEATLFVSGgPPGTGpvvlrVDR 334
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 387 N--------------------SIGTLMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEETAKAF------T 429
Cdd:cd05931  335 DalagravavaaddpaarelvSCGRPLPDQEVRIvdpetgrelpdGEVGEIWVRGPSVASGYWGRPEATAETFgalaatD 414
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....
gi 544685990 430 EDGFLRTGDVGEM-DscGNLFITDRLKELMkTSNGKYIAPQYIE 472
Cdd:cd05931  415 EGGWLRTGDLGFLhD--GELYITGRLKDLI-IVRGRNHYPQDIE 455
PRK06188 PRK06188
acyl-CoA synthetase; Validated
35-501 7.30e-26

acyl-CoA synthetase; Validated


Pssm-ID: 235731 [Multi-domain]  Cd Length: 524  Bit Score: 111.62  E-value: 7.30e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  35 EMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVg 114
Cdd:PRK06188  37 RLTYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLMAIGAAQLAGLRRTALHPLGSLDDHAYVLEDAGISTLIV- 115
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 115 DQEQF-DQVYQIANNCPQLMKIVAMKANMHLRDLPNACywedflnvvpneAEFE-KRLNSKQLS-DLFTLIYTSGTTGEP 191
Cdd:PRK06188 116 DPAPFvERALALLARVPSLKHVLTLGPVPDGVDLLAAA------------AKFGpAPLVAAALPpDIAGLAYTGGTTGKP 183
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 192 KGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIfERAWIAYVFHRGATncyledtkhvrdalttlkpTVMCavPR 271
Cdd:PRK06188 184 KGVMGTHRSIATMAQIQLAEWEWPADPRFLMCTPLSHA-GGAFFLPTLLRGGT-------------------VIVL--AK 241
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 272 FYEKiytAVWDKVEKaplHRralFNWAIRVgeqhyqieqPSQWlrlqYALAD-----KLVLTKLRALLGGRIKMMPcggA 346
Cdd:PRK06188 242 FDPA---EVLRAIEE---QR---ITATFLV---------PTMI----YALLDhpdlrTRDLSSLETVYYGASPMSP---V 296
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 347 KL-EA--SIGSFFHSIginiklgYGMTETTATVSCWQDKGFNPNSIGTL----MPNA------------EVKIGEENEIL 407
Cdd:PRK06188 297 RLaEAieRFGPIFAQY-------YGQTEAPMVITYLRKRDHDPDDPKRLtscgRPTPglrvalldedgrEVAQGEVGEIC 369
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 408 VRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:PRK06188 370 VRGPLVMDGYWNRPEETAEAF-RDGWLHTGDVAREDEDGFYYIVDRKKD-MIVTGGFNVFPREVEDVLAEHPAVAQVAVI 447
                        490
                 ....*....|....*...
gi 544685990 488 A--DAK--KYVSALIVPC 501
Cdd:PRK06188 448 GvpDEKwgEAVTAVVVLR 465
PRK06018 PRK06018
putative acyl-CoA synthetase; Provisional
47-486 1.37e-25

putative acyl-CoA synthetase; Provisional


Pssm-ID: 235673 [Multi-domain]  Cd Length: 542  Bit Score: 111.00  E-value: 1.37e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  47 RFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgDQEQFDQVYQIA 126
Cdd:PRK06018  51 KVSQALDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVNPRLFPEQIAWIINHAEDRVVIT-DLTFVPILEKIA 129
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 127 NNCPQLMKIVAM--KANMHLRDLPNACYWEDFLNVVPNE---AEFEKRLNSkqlsdlfTLIYTSGTTGEPKGVMLDY-AN 200
Cdd:PRK06018 130 DKLPSVERYVVLtdAAHMPQTTLKNAVAYEEWIAEADGDfawKTFDENTAA-------GMCYTSGTTGDPKGVLYSHrSN 202
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 201 LAHQLNAHDL-ALNVNEDDVSLSFLPLSHifERAW-IAYVFHRGATNCYLE----DTKHVRDALTTLKPTVMCAVPrfye 274
Cdd:PRK06018 203 VLHALMANNGdALGTSAADTMLPVVPLFH--ANSWgIAFSAPSMGTKLVMPgaklDGASVYELLDTEKVTFTAGVP---- 276
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 275 kiytAVWdkvekaplhrralfnwairvgeqhyqieqpsqWLRLQYALADKLVLTKLrallggriKMMPCGGAKLEASIGS 354
Cdd:PRK06018 277 ----TVW--------------------------------LMLLQYMEKEGLKLPHL--------KMVVCGGSAMPRSMIK 312
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 355 FFHSIGINIKLGYGMTETT--ATVSCWQD----------------KGFNPNSIgtlmpnaEVKI----GEE--------N 404
Cdd:PRK06018 313 AFEDMGVEVRHAWGMTEMSplGTLAALKPpfsklpgdarldvlqkQGYPPFGV-------EMKItddaGKElpwdgktfG 385
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 EILVRGGMVMRGYYKkpeETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNGKYIAPqyIEgkigkdkfIEQI 484
Cdd:PRK06018 386 RLKVRGPAVAAAYYR---VDGEILDDDGFFDTGDVATIDAYGYMRITDRSKDVIK-SGGEWISS--ID--------LENL 451

                 ..
gi 544685990 485 AV 486
Cdd:PRK06018 452 AV 453
PRK07059 PRK07059
Long-chain-fatty-acid--CoA ligase; Validated
43-487 2.14e-25

Long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235923 [Multi-domain]  Cd Length: 557  Bit Score: 110.50  E-value: 2.14e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  43 QEIDRFSYALIA--QHIDIQ--DKIGIFANNMPRWTIADFGAMQARAVAV---PIYatnTAKQVEYIVNDADIKILFVgd 115
Cdd:PRK07059  52 GELDELSRALAAwlQSRGLAkgARVAIMMPNVLQYPVAIAAVLRAGYVVVnvnPLY---TPRELEHQLKDSGAEAIVV-- 126
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 116 QEQF-DQVYQIANNCPQLMKIVAMKANMH----------LR---------DLPNACYWEDFLnvvpnEAEFEKRLNSKQL 175
Cdd:PRK07059 127 LENFaTTVQQVLAKTAVKHVVVASMGDLLgfkghivnfvVRrvkkmvpawSLPGHVRFNDAL-----AEGARQTFKPVKL 201
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 176 --SDLFTLIYTSGTTGEPKGVMLDYAN-LAHQLNA---HDLALNVNEDDVSLSF---LPLSHIFerawiayvfhrGATNC 246
Cdd:PRK07059 202 gpDDVAFLQYTGGTTGVSKGATLLHRNiVANVLQMeawLQPAFEKKPRPDQLNFvcaLPLYHIF-----------ALTVC 270
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 247 YLEdtkhvrdALTTLKPTVMCAVPRfyeKIYTAVwDKVEKAPLHR----RALFNwAIrvgeqhyqieqpsqwlrLQYALA 322
Cdd:PRK07059 271 GLL-------GMRTGGRNILIPNPR---DIPGFI-KELKKYQVHIfpavNTLYN-AL-----------------LNNPDF 321
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 323 DKLVLTKLRALLGGrikmmpcGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCwqdkgfNP-------NSIGTLMPN 395
Cdd:PRK07059 322 DKLDFSKLIVANGG-------GMAVQRPVAERWLEMTGCPITEGYGLSETSPVATC------NPvdatefsGTIGLPLPS 388
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 396 AEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKY 465
Cdd:PRK07059 389 TEVSIrdddgndlplGEPGEICIRGPQVMAGYWNRPDETAKVMTADGFFRTGDVGVMDERGYTKIVDRKKDMILVS-GFN 467
                        490       500
                 ....*....|....*....|..
gi 544685990 466 IAPQYIEGKIGKDKFIEQIAVI 487
Cdd:PRK07059 468 VYPNEIEEVVASHPGVLEVAAV 489
CHC_CoA_lg cd05903
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ...
37-535 5.35e-25

Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.


Pssm-ID: 341229 [Multi-domain]  Cd Length: 437  Bit Score: 107.85  E-value: 5.35e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  37 SWQTFQQEIDRFSYALIAQHIDIQDKIgifANNMPRW---TIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFV 113
Cdd:cd05903    3 TYSELDTRADRLAAGLAALGVGPGDVV---AFQLPNWwefAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVV 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 114 gdQEQFDQvyqianncpqlMKIVAMKAnmhlrdlpnacywedflnvvpneaefekrlnskqlsDLFTLIYTSGTTGEPKG 193
Cdd:cd05903   80 --PERFRQ-----------FDPAAMPD------------------------------------AVALLLFTSGTTGEPKG 110
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 194 VMLDYANL---AHQLNAHdlaLNVNEDDVSLSFLPLSHIferawIAYVFhrGATNCYLEDTkhvrdalttlkPTVMCAVp 270
Cdd:cd05903  111 VMHSHNTLsasIRQYAER---LGLGPGDVFLVASPMAHQ-----TGFVY--GFTLPLLLGA-----------PVVLQDI- 168
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 rfyekiytavWDKvekaplhrralfNWAIRVGEQHyqieqpsqwlRLQYALADKLVLTKL-RALLGG-----RIKMMPCG 344
Cdd:cd05903  169 ----------WDP------------DKALALMREH----------GVTFMMGATPFLTDLlNAVEEAgeplsRLRTFVCG 216
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 345 GAKLEASIGSFFHSIGINIKLG-YGMTET-TATVSCWQDK-GFNPNSIGTLMPNAEVKI----------GEENEILVRGG 411
Cdd:cd05903  217 GATVPRSLARRAAELLGAKVCSaYGSTECpGAVTSITPAPeDRRLYTDGRPLPGVEIKVvddtgatlapGVEGELLSRGP 296
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 412 MVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEGKIGKDKFIEQIAVIA--D 489
Cdd:cd05903  297 SVFLGYLDRPDLTADAA-PEGWFRTGDLARLDEDGYLRITGRSKDII-IRGGENIPVLEVEDLLLGHPGVIEAAVVAlpD 374
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 490 AK--KYVSALIV------PCFDSLEEY------AKQlniKYQDRIELIKH-----SDIIQMFERR 535
Cdd:cd05903  375 ERlgERACAVVVtksgalLTFDELVAYldrqgvAKQ---YWPERLVHVDDlprtpSGKVQKFRLR 436
PRK08974 PRK08974
long-chain-fatty-acid--CoA ligase FadD;
61-486 9.52e-25

long-chain-fatty-acid--CoA ligase FadD;


Pssm-ID: 236359 [Multi-domain]  Cd Length: 560  Bit Score: 108.60  E-value: 9.52e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  61 DKIGIFANNMPRWTIADFGAMQARAVAV---PIYA-------------------TNTAKQVEYIVNDADIK--ILF-VGD 115
Cdd:PRK08974  75 DRVALMMPNLLQYPIALFGILRAGMIVVnvnPLYTprelehqlndsgakaivivSNFAHTLEKVVFKTPVKhvILTrMGD 154
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 116 QEQFDQvYQIANNCPQLMKIVAMKanmhlRDLPNACYWEDFLNVVPNEAEFEKRLNSkqlSDLFTLIYTSGTTGEPKGVM 195
Cdd:PRK08974 155 QLSTAK-GTLVNFVVKYIKRLVPK-----YHLPDAISFRSALHKGRRMQYVKPELVP---EDLAFLQYTGGTTGVAKGAM 225
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 196 LDY----ANLAHQLNAHDLALNVNEDDVSLSfLPLSHIFERAWIAYVF-HRGATNCYLEDTKHVRDALTTLKPTVMCAVp 270
Cdd:PRK08974 226 LTHrnmlANLEQAKAAYGPLLHPGKELVVTA-LPLYHIFALTVNCLLFiELGGQNLLITNPRDIPGFVKELKKYPFTAI- 303
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 271 rfyekiyTAVwdkvekaplhrRALFNWAIRVGEQHyqieqpsqwlrlqyaladKLVLTKLRALLGGrikmmpcgGAKLEA 350
Cdd:PRK08974 304 -------TGV-----------NTLFNALLNNEEFQ------------------ELDFSSLKLSVGG--------GMAVQQ 339
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 351 SIGSFFHSI-GINIKLGYGMTETTATVSC--WQDKGFNpNSIGTLMPNAEVKI----------GEENEILVRGGMVMRGY 417
Cdd:PRK08974 340 AVAERWVKLtGQYLLEGYGLTECSPLVSVnpYDLDYYS-GSIGLPVPSTEIKLvdddgnevppGEPGELWVKGPQVMLGY 418
                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 418 YKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIG-KDKFIEQIAV 486
Cdd:PRK08974 419 WQRPEATDEVI-KDGWLATGDIAVMDEEGFLRIVDRKKDMILVS-GFNVYPNEIEDVVMlHPKVLEVAAV 486
PRK08180 PRK08180
feruloyl-CoA synthase; Reviewed
181-591 1.50e-24

feruloyl-CoA synthase; Reviewed


Pssm-ID: 236175 [Multi-domain]  Cd Length: 614  Bit Score: 108.04  E-value: 1.50e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDD--VSLSFLPLSHIF-ERAWIAYVFHRGATnCYLEDTKHVRDA 257
Cdd:PRK08180 214 FLFTSGSTGLPKAVINTHRMLCANQQMLAQTFPFLAEEppVLVDWLPWNHTFgGNHNLGIVLYNGGT-LYIDDGKPTPGG 292
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 258 L-TTLK------PTVMCAVPRFYEKIYTAvwdkVEKAPLHRRALFNwaiRVGEQHYQIEQPSQ--WLRLQyALAdklvlt 328
Cdd:PRK08180 293 FdETLRnlreisPTVYFNVPKGWEMLVPA----LERDAALRRRFFS---RLKLLFYAGAALSQdvWDRLD-RVA------ 358
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 klRALLGGRIKMMPcggakleasigsffhsiginiklGYGMTET--TATVSCWQDKGfnPNSIGTLMPNAEVKI---GEE 403
Cdd:PRK08180 359 --EATCGERIRMMT-----------------------GLGMTETapSATFTTGPLSR--AGNIGLPAPGCEVKLvpvGGK 411
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 404 NEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGD----VGEMD-SCGNLFitD-RLKELMKTSNGKYIApqyiegkIG- 476
Cdd:PRK08180 412 LEVRVKGPNVTPGYWRAPELTAEAFDEEGYYRSGDavrfVDPADpERGLMF--DgRIAEDFKLSSGTWVS-------VGp 482
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 477 -KDKFIEQIA-VIADA------KKYVSALIVPCFDSLEEYAKQLniKYQDRIELIKHSDIIQMFERRIHELQKELP-SFE 547
Cdd:PRK08180 483 lRARAVSAGApLVQDVvitghdRDEIGLLVFPNLDACRRLAGLL--ADASLAEVLAHPAVRAAFRERLARLNAQATgSST 560
                        410       420       430       440
                 ....*....|....*....|....*....|....*....|....
gi 544685990 548 QVKRFTLLPQAFSTAMEEITPTLKLRRKVIMQRYREQIEEMYNE 591
Cdd:PRK08180 561 RVARALLLDEPPSLDAGEITDKGYINQRAVLARRAALVEALYAD 604
PLN02330 PLN02330
4-coumarate--CoA ligase-like 1
12-487 7.92e-24

4-coumarate--CoA ligase-like 1


Pssm-ID: 215189 [Multi-domain]  Cd Length: 546  Bit Score: 105.45  E-value: 7.92e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  12 FRLQ-AKKWLNRTAlrFREQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI 90
Cdd:PLN02330  33 FVLQdAELYADKVA--FVEAVTGKAVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGVFSGA 110
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  91 YATNTAKQVEYIVNDADIKiLFVGDQEQFDQVYQIanncpQLMKIVAMKANmhlrdLPNACYWEDFLNVVPNEAEfeKRL 170
Cdd:PLN02330 111 NPTALESEIKKQAEAAGAK-LIVTNDTNYGKVKGL-----GLPVIVLGEEK-----IEGAVNWKELLEAADRAGD--TSD 177
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 171 NSKQL-SDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNE--DDVSLSFLPLSHIFERAWIAYVFHRGatncy 247
Cdd:PLN02330 178 NEEILqTDLCALPFSSGTTGISKGVMLTHRNLVANLCSSLFSVGPEMigQVVTLGLIPFFHIYGITGICCATLRN----- 252
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 248 ledtkhvrdalttlKPTVMcAVPRFYEKIY--TAVWDKVEKAPLHRRALFNWairvgeqhyqIEQPsqwlrlqyaLADKL 325
Cdd:PLN02330 253 --------------KGKVV-VMSRFELRTFlnALITQEVSFAPIVPPIILNL----------VKNP---------IVEEF 298
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 326 VLTKLRallggrIKMMPCGGAKLEASIGSFFHSI--GINIKLGYGMTETTATVSCWQD--KGF---NPNSIGTLMPNAEV 398
Cdd:PLN02330 299 DLSKLK------LQAIMTAAAPLAPELLTAFEAKfpGVQVQEAYGLTEHSCITLTHGDpeKGHgiaKKNSVGFILPNLEV 372
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 399 K-IGEEN----------EILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIA 467
Cdd:PLN02330 373 KfIDPDTgrslpkntpgELCVRSQCVMQGYYNNKEETDRTIDEDGWLHTGDIGYIDDDGDIFIVDRIKELIKY-KGFQVA 451
                        490       500
                 ....*....|....*....|
gi 544685990 468 PQYIEGKIGKDKFIEQIAVI 487
Cdd:PLN02330 452 PAELEAILLTHPSVEDAAVV 471
EntF COG1020
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ...
11-448 9.76e-24

EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440643 [Multi-domain]  Cd Length: 1329  Bit Score: 106.48  E-value: 9.76e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   11 RFRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFannMPR---WTIADFGAMQARAVA 87
Cdd:COG1020   481 LFEAQAARTPDAVAVVFGDQ----SLTYAELNARANRLAHHLRALGVGPGDLVGVC---LERsleMVVALLAVLKAGAAY 553
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   88 VPIYATNTAKQVEYIVNDADIKILfVGDQEQFDQVYQIAnncpqlMKIVAMkanmhlrDLPNACYWEDFLNVVPNEAEfe 167
Cdd:COG1020   554 VPLDPAYPAERLAYMLEDAGARLV-LTQSALAARLPELG------VPVLAL-------DALALAAEPATNPPVPVTPD-- 617
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  168 krlnskqlsDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSH------IFeRAWIAyvfhr 241
Cdd:COG1020   618 ---------DLAYVIYTSGSTGRPKGVMVEHRALVNLLAWMQRRYGLGPGDRVLQFASLSFdasvweIF-GALLS----- 682
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  242 GATnCYLEDTKHVRDA------LTTLKPTVMCAVPrfyekiytAVWdkvekaplhrRALfnwairvgeqhyqieqpsqwl 315
Cdd:COG1020   683 GAT-LVLAPPEARRDPaalaelLARHRVTVLNLTP--------SLL----------RAL--------------------- 722
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  316 rLQYALADklvLTKLRALLggrikmmpCGGAKLEASIGSFFHSIGINIKL--GYGMTETTATVSCWQ----DKGFNPNSI 389
Cdd:COG1020   723 -LDAAPEA---LPSLRLVL--------VGGEALPPELVRRWRARLPGARLvnLYGPTETTVDSTYYEvtppDADGGSVPI 790
                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990  390 GTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFL-------RTGDVGEMDSCGNL 448
Cdd:COG1020   791 GRPIANTRVYVldahlqpvpvGVPGELYIGGAGLARGYLNRPELTAERFVADPFGfpgarlyRTGDLARWLPDGNL 866
PRK07529 PRK07529
AMP-binding domain protein; Validated
11-472 3.54e-23

AMP-binding domain protein; Validated


Pssm-ID: 236043 [Multi-domain]  Cd Length: 632  Bit Score: 103.88  E-value: 3.54e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  11 RFRLQAKKWLNRTALRF-REQAQWQE---MSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAmQARAV 86
Cdd:PRK07529  30 LLSRAAARHPDAPALSFlLDADPLDRpetWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWGG-EAAGI 108
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  87 AVPIYATNTAKQVEYIVNDADIKILF-VG---DQEQFDQVYQIANNCPQLMKIVAMKANmhlRDLPNACYWE-------- 154
Cdd:PRK07529 109 ANPINPLLEPEQIAELLRAAGAKVLVtLGpfpGTDIWQKVAEVLAALPELRTVVEVDLA---RYLPGPKRLAvplirrka 185
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 155 -----DFLNVVPNE----AEFEKRLNSKQLSDLFtliYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLP 225
Cdd:PRK07529 186 harilDFDAELARQpgdrLFSGRPIGPDDVAAYF---HTGGTTGMPKLAQHTHGNEVANAWLGALLLGLGPGDTVFCGLP 262
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 226 LSHIFErawiAYV-----FHRGATncyledtkhvrdalttlkptVMCAVPRFY--EKIYTAVWDKVEkaplhrralfnwa 298
Cdd:PRK07529 263 LFHVNA----LLVtglapLARGAH--------------------VVLATPQGYrgPGVIANFWKIVE------------- 305
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 299 irvgeqHYQIEQPSQwlrlqyaladklVLTKLRALL----GGR----IKMMPCGGAKLEASIG-SFFHSIGINIKLGYGM 369
Cdd:PRK07529 306 ------RYRINFLSG------------VPTVYAALLqvpvDGHdissLRYALCGAAPLPVEVFrRFEAATGVRIVEGYGL 367
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 370 TETTATVSCwqdkgfNP-------NSIGTLMPNAEVKI---------------GEENEILVRGGMVMRGYYKkPEETAKA 427
Cdd:PRK07529 368 TEATCVSSV------NPpdgerriGSVGLRLPYQRVRVvilddagrylrdcavDEVGVLCIAGPNVFSGYLE-AAHNKGL 440
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*
gi 544685990 428 FTEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIE 472
Cdd:PRK07529 441 WLEDGWLNTGDLGRIDADGYFWLTGRAKDLI-IRGGHNIDPAAIE 484
ACLS-CaiC cd17637
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ...
177-491 4.89e-23

acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341292 [Multi-domain]  Cd Length: 333  Bit Score: 100.42  E-value: 4.89e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANL---AHQLNahdLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLE--DT 251
Cdd:cd17637    1 DPFVIIHTAAVAGRPRGAVLSHGNLiaaNLQLI---HAMGLTEADVYLNMLPLFHIAGLNLALATFHAGGANVVMEkfDP 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 252 KHVRDALTTLKPTVMCAVPrfyeKIYTAVWDKVEKAPLHrralfnwairvgeqhyqieqpsqwlrlqyaladklvLTKLR 331
Cdd:cd17637   78 AEALELIEEEKVTLMGSFP----PILSNLLDAAEKSGVD------------------------------------LSSLR 117
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 332 ALLGGRikmMPCGGAKLEASIGSFFHSiginiklGYGMTETT--ATVSCWQDKgfnPNSIGTLMPNAEVKI--------- 400
Cdd:cd17637  118 HVLGLD---APETIQRFEETTGATFWS-------LYGQTETSglVTLSPYRER---PGSAGRPGPLVRVRIvddndrpvp 184
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -GEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRL--KELMKTSnGKYIAPQYIEGKIGK 477
Cdd:cd17637  185 aGETGEIVVRGPLVFQGYWNLPELTAYTF-RNGWHHTGDLGRFDEDGYLWYAGRKpeKELIKPG-GENVYPAEVEKVILE 262
                        330
                 ....*....|....*.
gi 544685990 478 DKFIEQIAVI--ADAK 491
Cdd:cd17637  263 HPAIAEVCVIgvPDPK 278
DltA cd05945
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ...
21-499 1.04e-22

D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341267 [Multi-domain]  Cd Length: 449  Bit Score: 101.17  E-value: 1.04e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:cd05945    6 DRPAVVEGGR----TLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAERIR 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFV-GDqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqlsDLF 179
Cdd:cd05945   82 EILDAAKPALLIAdGD-------------------------------------------------------------DNA 100
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 180 TLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS------HIFErAWIAyvfhrGATnCYL---ED 250
Cdd:cd05945  101 YIIFTSGSTGRPKGVQISHDNLVSFTNWMLSDFPLGPGDVFLNQAPFSfdlsvmDLYP-ALAS-----GAT-LVPvprDA 173
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 251 TKHVRDALTTLKP---TVMCAVPRFYEKIYTAVWDKVEKAPLHRRALFnwairVGEQhyqieqpsqwlrLQYALADKLVl 327
Cdd:cd05945  174 TADPKQLFRFLAEhgiTVWVSTPSFAAMCLLSPTFTPESLPSLRHFLF-----CGEV------------LPHKTARALQ- 235
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 328 tklRALLGGRIkmmpcggakleasigsffhsigINIklgYGMTETTATVSCWQ-----DKGFNPNSIGTLMPNAEVKI-- 400
Cdd:cd05945  236 ---QRFPDARI----------------------YNT---YGPTEATVAVTYIEvtpevLDGYDRLPIGYAKPGAKLVIld 287
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 --------GEENEILVRGGMVMRGYYKKPEETAKAFTED---GFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQ 469
Cdd:cd05945  288 edgrpvppGEKGELVISGPSVSKGYLNNPEKTAAAFFPDegqRAYRTGDLVRLEADGLLFYRGRLDFQVKL-NGYRIELE 366
                        490       500       510
                 ....*....|....*....|....*....|.
gi 544685990 470 YIEGKIGKDKFIEQIAVIADAKK-YVSALIV 499
Cdd:cd05945  367 EIEAALRQVPGVKEAVVVPKYKGeKVTELIA 397
PRK09088 PRK09088
acyl-CoA synthetase; Validated
61-491 1.18e-22

acyl-CoA synthetase; Validated


Pssm-ID: 181644 [Multi-domain]  Cd Length: 488  Bit Score: 101.42  E-value: 1.18e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  61 DKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILfVGDQeqfdqvyQIANNCPQLMKIVAMKA 140
Cdd:PRK09088  48 ERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDALLQDAEPRLL-LGDD-------AVAAGRTDVEDLAAFIA 119
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 141 NmhlrdlpnacywedflnVVPNEAEFEKRLNSKQLSdlfTLIYTSGTTGEPKGVMLDYANLAHqlNAHDLALNVNEDDVS 220
Cdd:PRK09088 120 S-----------------ADALEPADTPSIPPERVS---LILFTSGTSGQPKGVMLSERNLQQ--TAHNFGVLGRVDAHS 177
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 221 lSFLPLSHIFerawiayvfhrgatncyledtkHVRDALTTLKPTVMCA----VPRFYEKIYTAVWdkvekapLHRRALfn 296
Cdd:PRK09088 178 -SFLCDAPMF----------------------HIIGLITSVRPVLAVGgsilVSNGFEPKRTLGR-------LGDPAL-- 225
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 297 wairvGEQHY-QIEQPSQWLRLQYALaDKLVLTKLRALLGGrikmmpcGGAKLEASIGSFFHSiGINIKLGYGMTETTAT 375
Cdd:PRK09088 226 -----GITHYfCVPQMAQAFRAQPGF-DAAALRHLTALFTG-------GAPHAAEDILGWLDD-GIPMVDGFGMSEAGTV 291
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 376 VSCWQDKGFNPN---SIGTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEM 442
Cdd:PRK09088 292 FGMSVDCDVIRAkagAAGIPTPTVQTRVvddqgndcpaGVPGELLLRGPNLSPGYWRRPQATARAFTGDGWFRTGDIARR 371
                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|.
gi 544685990 443 DSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDKFIEQIAVI--ADAK 491
Cdd:PRK09088 372 DADGFFWVVDRKKD-MFISGGENVYPAEIEAVLADHPGIRECAVVgmADAQ 421
PRK07470 PRK07470
acyl-CoA synthetase; Validated
20-491 1.55e-22

acyl-CoA synthetase; Validated


Pssm-ID: 180988 [Multi-domain]  Cd Length: 528  Bit Score: 101.27  E-value: 1.55e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  20 LNRTALRFRE-------QAQWqemSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYA 92
Cdd:PRK07470  13 LRQAARRFPDrialvwgDRSW---TWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVWVPTNF 89
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  93 TNTAKQVEYIVNDADIKiLFVGDQEQFDQVYQIANNCPQLMKIVAMKAnmhlrdlpnACYWEDFLNVVPNEAEFEKRLNS 172
Cdd:PRK07470  90 RQTPDEVAYLAEASGAR-AMICHADFPEHAAAVRAASPDLTHVVAIGG---------ARAGLDYEALVARHLGARVANAA 159
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 173 KQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAH--DLALNVNEDDVSLSFLPLSH---IFERAWIAyvfhRGATNCY 247
Cdd:PRK07470 160 VDHDDPCWFFFTSGTTGRPKAAVLTHGQMAFVITNHlaDLMPGTTEQDASLVVAPLSHgagIHQLCQVA----RGAATVL 235
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 248 LEDTKHVRDALTTL----KPTVMCAVPrfyekiyTAVWDKVEKAPLHRR--ALFNWAIRVGEQHYQIEQpsqwlrlQYAL 321
Cdd:PRK07470 236 LPSERFDPAEVWALverhRVTNLFTVP-------TILKMLVEHPAVDRYdhSSLRYVIYAGAPMYRADQ-------KRAL 301
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 322 AdKL--VLTKLRAL--LGGRIKMMPcggakleasigSFFHSI--GINIKLGY-GMTETTATVSCWQDKGfnpnsigtlmp 394
Cdd:PRK07470 302 A-KLgkVLVQYFGLgeVTGNITVLP-----------PALHDAedGPDARIGTcGFERTGMEVQIQDDEG----------- 358
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 395 nAEVKIGEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGK 474
Cdd:PRK07470 359 -RELPPGETGEICVIGPAVFAGYYNNPEANAKAF-RDGWFRTGDLGHLDARGFLYITGRASD-MYISGGSNVYPREIEEK 435
                        490
                 ....*....|....*....
gi 544685990 475 IGKDKFIEQIAV--IADAK 491
Cdd:PRK07470 436 LLTHPAVSEVAVlgVPDPV 454
A_NRPS_TubE_like cd05906
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ...
177-457 2.77e-22

The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341232 [Multi-domain]  Cd Length: 540  Bit Score: 100.82  E-value: 2.77e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIferAWIAYVfhrgatncyledtkHVRD 256
Cdd:cd05906  168 DLALLMLTSGSTGFPKAVPLTHRNILARSAGKIQHNGLTPQDVFLNWVPLDHV---GGLVEL--------------HLRA 230
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 257 ALTtlkptvMCavprfyEKIYTAVwdkvekaplhrralfnwairvgeqHYQIEQPSQWLRL------QYALADKLVLTKL 330
Cdd:cd05906  231 VYL------GC------QQVHVPT------------------------EEILADPLRWLDLidryrvTITWAPNFAFALL 274
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 331 RALLG---------GRIKMMPCGGAKLEASIGSFFHSI----GIN---IKLGYGMTETTA----TVSCWQDKGFNPN--- 387
Cdd:cd05906  275 NDLLEeiedgtwdlSSLRYLVNAGEAVVAKTIRRLLRLlepyGLPpdaIRPAFGMTETCSgviySRSFPTYDHSQALefv 354
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 388 SIGTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDScGNLFITDRLKEL 457
Cdd:cd05906  355 SLGRPIPGVSMRIvddegqllpeGEVGRLQVRGPVVTKGYYNNPEANAEAFTEDGWFRTGDLGFLDN-GNLTITGRTKDT 433
A_NRPS_Ta1_like cd12116
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ...
21-575 4.05e-22

The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.


Pssm-ID: 341281 [Multi-domain]  Cd Length: 470  Bit Score: 99.67  E-value: 4.05e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:cd12116    2 DATAVRDDDR----SLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLR 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILfVGDQEQFDQVyqiANNCPQLMKIVAMKANMHLRDLPNacywedflnVVPNeaefekrlnskqlsDLFT 180
Cdd:cd12116   78 YILEDAEPALV-LTDDALPDRL---PAGLPVLLLALAAAAAAPAAPRTP---------VSPD--------------DLAY 130
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLS-------------FLPLSHiferawiayvfhrGATnCY 247
Cdd:cd12116  131 VIYTSGSTGRPKGVVVSHRNLVNFLHSMRERLGLGPGDRLLAvttyafdisllelLLPLLA-------------GAR-VV 196
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 248 LEDTKHVRDA------LTTLKPTVMCAVPrfyekiytAVWdkvekaplhrRALFNwairvgeqhyqieqpSQWLRLQyal 321
Cdd:cd12116  197 IAPRETQRDPealarlIEAHSITVMQATP--------ATW----------RMLLD---------------AGWQGRA--- 240
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 322 adklvltKLRALlggrikmmpCGGAKLEASIGSFFHSIG---INIklgYGMTETTaTVSCWQ--DKGFNPNSIGTLMPNA 396
Cdd:cd12116  241 -------GLTAL---------CGGEALPPDLAARLLSRVgslWNL---YGPTETT-IWSTAArvTAAAGPIPIGRPLANT 300
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 397 E----------VKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFL-------RTGDVGEMDSCGNLFITDRLKELMK 459
Cdd:cd12116  301 QvyvldaalrpVPPGVPGELYIGGDGVAQGYLGRPALTAERFVPDPFAgpgsrlyRTGDLVRRRADGRLEYLGRADGQVK 380
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 460 TsNGKYIAPQYIEGKIGKDKFIEQIAVIA---DAKKYVSALIVPCFDSLeeyakqlnikyQDRIELIKHsdiiqmferri 536
Cdd:cd12116  381 I-RGHRIELGEIEAALAAHPGVAQAAVVVredGGDRRLVAYVVLKAGAA-----------PDAAALRAH----------- 437
                        570       580       590       600
                 ....*....|....*....|....*....|....*....|.
gi 544685990 537 heLQKELPSFeqvkrftLLPQAFsTAMEEI--TPTLKLRRK 575
Cdd:cd12116  438 --LRATLPAY-------MVPSAF-VRLDALplTANGKLDRK 468
MACS_AAE_MA_like cd05970
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ...
85-580 4.10e-22

Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.


Pssm-ID: 341274 [Multi-domain]  Cd Length: 537  Bit Score: 100.26  E-value: 4.10e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  85 AVAVPIYATNTAKQVEYIVNDADIKILFVGDQEQFDQVYQIAN-NCPQLMKIVAMKAnmhlrDLPNAcyWEDFLNVVPNE 163
Cdd:cd05970   97 AIAIPATHQLTAKDIVYRIESADIKMIVAIAEDNIPEEIEKAApECPSKPKLVWVGD-----PVPEG--WIDFRKLIKNA 169
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 164 AEFEKR--LNSKQLSDLFTLIY-TSGTTGEPKGVMLDYA-NLAHQLNAHdLALNVNEDDVSLSflplshIFERAWIAYVF 239
Cdd:cd05970  170 SPDFERptANSYPCGEDILLVYfSSGTTGMPKMVEHDFTyPLGHIVTAK-YWQNVREGGLHLT------VADTGWGKAVW 242
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 240 HR------GATNCYLED-----TKHVRDALTTLKPTVMCAVPRFYekiytavwdkvekaplhrRALfnwaIRVGEQHYQI 308
Cdd:cd05970  243 GKiygqwiAGAAVFVYDydkfdPKALLEKLSKYGVTTFCAPPTIY------------------RFL----IREDLSRYDL 300
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 309 EQpsqwlrLQYALAdklvltklrallggrikmmpCGGAKLEASIGSFFHSIGINIKLGYGMTETT---ATVSCWQDKgfn 385
Cdd:cd05970  301 SS------LRYCTT--------------------AGEALNPEVFNTFKEKTGIKLMEGFGQTETTltiATFPWMEPK--- 351
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 386 PNSIGTLMPN----------AEVKIGEENEILVRG------GMvMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLF 449
Cdd:cd05970  352 PGSMGKPAPGyeidlidregRSCEAGEEGEIVIRTskgkpvGL-FGGYYKDAEKTAEVW-HDGYYHTGDAAWMDEDGYLW 429
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 450 ITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAV--IADAKK--YVSALIVpcfdsleeyakqLNIKYQDRIELIKH 525
Cdd:cd05970  430 FVGRTDDLIKSS-GYRIGPFEVESALIQHPAVLECAVtgVPDPIRgqVVKATIV------------LAKGYEPSEELKKE 496
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 526 sdiIQMFERRIHELQKELPSFEQVKRftlLPQAFSTameeitptlKLRRKVIMQR 580
Cdd:cd05970  497 ---LQDHVKKVTAPYKYPRIVEFVDE---LPKTISG---------KIRRVEIRER 536
PRK06087 PRK06087
medium-chain fatty-acid--CoA ligase;
47-488 6.92e-22

medium-chain fatty-acid--CoA ligase;


Pssm-ID: 180393 [Multi-domain]  Cd Length: 547  Bit Score: 99.44  E-value: 6.92e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  47 RFSYALIAQHIDIQDKIgifANNMPRW---TIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQEQ----F 119
Cdd:PRK06087  61 RLANWLLAKGIEPGDRV---AFQLPGWcefTIIYLACLKVGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTLFKqtrpV 137
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 120 DQVYQIANNCPQLMKIVAM-KANMHLRDLPNACYWEDF--LNVVPNEAEfekrlnskqlSDLFTLIYTSGTTGEPKGVML 196
Cdd:PRK06087 138 DLILPLQNQLPQLQQIVGVdKLAPATSSLSLSQIIADYepLTTAITTHG----------DELAAVLFTSGTEGLPKGVML 207
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 197 DYANLAHQLNAHDLALNVNEDDVSLSFLPLSHiferawiAYVFHRGATNCYLEDTKHV-------RDALTTL---KPT-V 265
Cdd:PRK06087 208 THNNILASERAYCARLNLTWQDVFMMPAPLGH-------ATGFLHGVTAPFLIGARSVlldiftpDACLALLeqqRCTcM 280
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 266 MCAVPRFYEKIYTavwdkVEKAPLHrralfnwairvgeqhyqieqpsqwlrlqyaladklvLTKLRALLggrikmmpCGG 345
Cdd:PRK06087 281 LGATPFIYDLLNL-----LEKQPAD------------------------------------LSALRFFL--------CGG 311
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 346 AKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDK--GFNPNSIGTLMPNAEVKI----------GEENEILVRGGMV 413
Cdd:PRK06087 312 TTIPKKVARECQQRGIKLLSVYGSTESSPHAVVNLDDplSRFMHTDGYAAAGVEIKVvdearktlppGCEGEEASRGPNV 391
                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 414 MRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:PRK06087 392 FMGYLDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDII-VRGGENISSREVEDILLQHPKIHDACVVA 465
PRK07514 PRK07514
malonyl-CoA synthase; Validated
50-500 1.15e-21

malonyl-CoA synthase; Validated


Pssm-ID: 181011 [Multi-domain]  Cd Length: 504  Bit Score: 98.41  E-value: 1.15e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  50 YALIAQHIDIQDKIGIFANNMPRWTIADFGAMQAR----------------AV-------AVPIY-AT----------NT 95
Cdd:PRK07514   6 FDALRAAFADRDAPFIETPDGLRYTYGDLDAASARlanllvalgvkpgdrvAVqvekspeALALYlATlragavflplNT 85
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  96 AKQ---VEYIVNDADIKiLFVGDQEQFDQVYQIANNC--PQLMKIVA------MKANMHLRDlpnacyweDFlNVVPNEA 164
Cdd:PRK07514  86 AYTlaeLDYFIGDAEPA-LVVCDPANFAWLSKIAAAAgaPHVETLDAdgtgslLEAAAAAPD--------DF-ETVPRGA 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 165 EfekrlnskqlsDLFTLIYTSGTTGEPKGVMLDYANLAHqlNAhdLALN----VNEDDVSLSFLPlshiferawiayVFH 240
Cdd:PRK07514 156 D-----------DLAAILYTSGTTGRSKGAMLSHGNLLS--NA--LTLVdywrFTPDDVLIHALP------------IFH 208
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 241 -RG---ATNCYLE-----------DTKHVRDALTtlKPTVMCAVPRFYekiytavwdkvekaplhrralfnwairvgeqh 305
Cdd:PRK07514 209 tHGlfvATNVALLagasmiflpkfDPDAVLALMP--RATVMMGVPTFY-------------------------------- 254
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 306 yqieqpsqwLRLqyaLADKlVLTKLRAllgGRIKMMPCGGAKLEASI-GSFFHSIGINIKLGYGMTETTATVScwqdkgf 384
Cdd:PRK07514 255 ---------TRL---LQEP-RLTREAA---AHMRLFISGSAPLLAEThREFQERTGHAILERYGMTETNMNTS------- 311
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 385 NP-------NSIGTLMPNAEVKI-----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCG 446
Cdd:PRK07514 312 NPydgerraGTVGFPLPGVSLRVtdpetgaelppGEIGMIEVKGPNVFKGYWRMPEKTAEEFRADGFFITGDLGKIDERG 391
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 544685990 447 NLFITDRLKELMkTSNGKYIAPQYIEGkigkdkFIEQI------AVI----ADAKKYVSALIVP 500
Cdd:PRK07514 392 YVHIVGRGKDLI-ISGGYNVYPKEVEG------EIDELpgvvesAVIgvphPDFGEGVTAVVVP 448
A_NRPS_Bac cd17655
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ...
11-575 4.24e-21

bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341310 [Multi-domain]  Cd Length: 490  Bit Score: 96.63  E-value: 4.24e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  11 RFRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI 90
Cdd:cd17655    2 LFEEQAEKTPDHTAVVFEDQ----TLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPI 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  91 YATNTAKQVEYIVNDADIKILFVgdQEQFDQvyqianncpqlmKIVAMKANMHLRDLPNACYWEDFLNVVPNEaefekrl 170
Cdd:cd17655   78 DPDYPEERIQYILEDSGADILLT--QSHLQP------------PIAFIGLIDLLDEDTIYHEESENLEPVSKS------- 136
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 171 nskqlSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLShiFErAWIAYVFHR---GATNC- 246
Cdd:cd17655  137 -----DDLAYVIYTSGSTGKPKGVMIEHRGVVNLVEWANKVIYQGEHLRVALFASIS--FD-ASVTEIFASllsGNTLYi 208
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 247 YLEDTKHVRDALTtlkptvmcavpRFYEKIYTAVwdkVEKAPLHrralfnwairvgeqhyqieqpsqwlrLQyaladklV 326
Cdd:cd17655  209 VRKETVLDGQALT-----------QYIRQNRITI---IDLTPAH--------------------------LK-------L 241
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 327 LTKLRALLGGRIKMMPCGGAKLEAS-IGSFFHSIGINIKL--GYGMTETTATVSCWQ-----DKGFNPnSIGTLMPNAEV 398
Cdd:cd17655  242 LDAADDSEGLSLKHLIVGGEALSTElAKKIIELFGTNPTItnAYGPTETTVDASIYQyepetDQQVSV-PIGKPLGNTRI 320
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 399 KI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFL------RTGDVGEMDSCGNLFITDRLKELMKTsN 462
Cdd:cd17655  321 YIldqygrpqpvGVAGELYIGGEGVARGYLNRPELTAEKFVDDPFVpgermyRTGDLARWLPDGNIEFLGRIDHQVKI-R 399
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 463 GKYIAPQYIEGKIGKDKFIEQIAVIA----DAKKYVSALIVpcFDsleeyakqlnikyqdrieliKHSDIIQMFERrihe 538
Cdd:cd17655  400 GYRIELGEIEARLLQHPDIKEAVVIArkdeQGQNYLCAYIV--SE--------------------KELPVAQLREF---- 453
                        570       580       590
                 ....*....|....*....|....*....|....*....
gi 544685990 539 LQKELPSFeqvkrftLLPqAFSTAMEEI--TPTLKLRRK 575
Cdd:cd17655  454 LARELPDY-------MIP-SYFIKLDEIplTPNGKVDRK 484
A_NRPS_MycA_like cd05908
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ...
173-472 4.87e-21

The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341234 [Multi-domain]  Cd Length: 499  Bit Score: 96.79  E-value: 4.87e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 173 KQLSDLFTLI-YTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHifERAWIAyvFH-----RGATNC 246
Cdd:cd05908  102 CELADELAFIqFSSGSTGDPKGVMLTHENLVHNMFAILNSTEWKTKDRILSWMPLTH--DMGLIA--FHlapliAGMNQY 177
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 247 YLEDTKHVRDALTTL------KPTVMCAvPRFYEKIYTAVWdKVEKAP----LHRRALFNWAirvgeqhyqieQPsqwlr 316
Cdd:cd05908  178 LMPTRLFIRRPILWLkkasehKATIVSS-PNFGYKYFLKTL-KPEKANdwdlSSIRMILNGA-----------EP----- 239
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 317 LQYALADKLvLTKLRALLGGRIKMMPCGGAKlEASIGSFFHSIGINIK----------LGYGMTETTATVScwqdKGFNP 386
Cdd:cd05908  240 IDYELCHEF-LDHMSKYGLKRNAILPVYGLA-EASVGASLPKAQSPFKtitlgrrhvtHGEPEPEVDKKDS----ECLTF 313
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 387 NSIGTLMPNAEVKI-GEENEIL---------VRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDScGNLFITDRLKE 456
Cdd:cd05908  314 VEVGKPIDETDIRIcDEDNKILpdgyighiqIRGKNVTPGYYNNPEATAKVFTDDGWLKTGDLGFIRN-GRLVITGREKD 392
                        330
                 ....*....|....*.
gi 544685990 457 LMKTsNGKYIAPQYIE 472
Cdd:cd05908  393 IIFV-NGQNVYPHDIE 407
PRK12492 PRK12492
long-chain-fatty-acid--CoA ligase; Provisional
22-472 6.95e-21

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 171539 [Multi-domain]  Cd Length: 562  Bit Score: 96.43  E-value: 6.95e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  22 RTALRFREQAQWQEMSWQTFQQEIDRFSYALIA---QHIDIQ--DKIGIFANNMPRWTIADFGAMQARAVAV---PIYAT 93
Cdd:PRK12492  32 RSCKKFADRPAFSNLGVTLSYAELERHSAAFAAylqQHTDLVpgDRIAVQMPNVLQYPIAVFGALRAGLIVVntnPLYTA 111
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  94 NT-------------------AKQVEYIVNDADIKILF---VGDQEQFDQVYQIANNCPQLMKIVAmkaNMHLrdlPNAC 151
Cdd:PRK12492 112 REmrhqfkdsgaralvylnmfGKLVQEVLPDTGIEYLIeakMGDLLPAAKGWLVNTVVDKVKKMVP---AYHL---PQAV 185
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 152 yweDFLNVVPNEAEFEKRLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLA----------HQLNAHDLALNVNEDDVSL 221
Cdd:PRK12492 186 ---PFKQALRQGRGLSLKPVPVGLDDIAVLQYTGGTTGLAKGAMLTHGNLVanmlqvraclSQLGPDGQPLMKEGQEVMI 262
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 222 SFLPLSHIferawiaYVFhrgATNCYLedtkhvrdALTTLKPTVMCAVPRfyekiytavwdkveKAPLHRRALFNWairv 301
Cdd:PRK12492 263 APLPLYHI-------YAF---TANCMC--------MMVSGNHNVLITNPR--------------DIPGFIKELGKW---- 306
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 302 geqhyqieQPSQWLRLQYALADKLVLTKLRALLGGRIKMMPCGGAKLEASIGSFFHSI-GINIKLGYGMTETTATVSCwq 380
Cdd:PRK12492 307 --------RFSALLGLNTLFVALMDHPGFKDLDFSALKLTNSGGTALVKATAERWEQLtGCTIVEGYGLTETSPVAST-- 376
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 381 dkgfNP-------NSIGTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMD 443
Cdd:PRK12492 377 ----NPygelarlGTVGIPVPGTALKVidddgnelplGERGELCIKGPQVMKGYWQQPEATAEALDAEGWFKTGDIAVID 452
                        490       500
                 ....*....|....*....|....*....
gi 544685990 444 SCGNLFITDRLKELMKTSnGKYIAPQYIE 472
Cdd:PRK12492 453 PDGFVRIVDRKKDLIIVS-GFNVYPNEIE 480
PRK07786 PRK07786
long-chain-fatty-acid--CoA ligase; Validated
7-491 3.70e-20

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 169098 [Multi-domain]  Cd Length: 542  Bit Score: 94.07  E-value: 3.70e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   7 HFVNRFRLQAKKWLNRTALRFREQAqwqeMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAV 86
Cdd:PRK07786  18 NWVNQLARHALMQPDAPALRFLGNT----TTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAI 93
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  87 AVPIYATNTAKQVEYIVNDADIKILFVGDQEQfDQVYQIANNCPQLMKIVAMKANMHLRDLPnacyWEDFLNV------- 159
Cdd:PRK07786  94 AVPVNFRLTPPEIAFLVSDCGAHVVVTEAALA-PVATAVRDIVPLLSTVVVAGGSSDDSVLG----YEDLLAEagpahap 168
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 160 --VPNEAEfekrlnskqlsdlfTLI-YTSGTTGEPKGVMLDYANLAHQLNAHDLALNVN-EDDVSLSFLPLSHIFERAWI 235
Cdd:PRK07786 169 vdIPNDSP--------------ALImYTSGTTGRPKGAVLTHANLTGQAMTCLRTNGADiNSDVGFVGVPLFHIAGIGSM 234
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 236 AYVFHRGATNCYLE----DTKHVRDALTTLKPTVMCAVPrfyekiytAVWDKVEKAPLHR-RALfnwAIRVgeqhyqieq 310
Cdd:PRK07786 235 LPGLLLGAPTVIYPlgafDPGQLLDVLEAEKVTGIFLVP--------AQWQAVCAEQQARpRDL---ALRV--------- 294
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 311 psqwLRLQYALADKLVLTKLRAllggrikMMPcggaklEASIGSFFhsiginiklgyGMTETTAtVSCW---QDKGFNPN 387
Cdd:PRK07786 295 ----LSWGAAPASDTLLRQMAA-------TFP------EAQILAAF-----------GQTEMSP-VTCMllgEDAIRKLG 345
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 388 SIGTLMPNA----------EVKIGEENEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKEl 457
Cdd:PRK07786 346 SVGKVIPTVaarvvdenmnDVPVGEVGEIVYRAPTLMSGYWNNPEATAEAF-AGGWFHSGDLVRQDEEGYVWVVDRKKD- 423
                        490       500       510
                 ....*....|....*....|....*....|....*.
gi 544685990 458 MKTSNGKYIAPQYIEGKIGKDKFIEQIAVI--ADAK 491
Cdd:PRK07786 424 MIISGGENIYCAEVENVLASHPDIVEVAVIgrADEK 459
BCL_4HBCL cd05959
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ...
42-511 1.43e-19

Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.


Pssm-ID: 341269 [Multi-domain]  Cd Length: 508  Bit Score: 92.05  E-value: 1.43e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  42 QQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgdQEQFDQ 121
Cdd:cd05959   36 EAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPVPVNTLLTPDDYAYYLEDSRARVVVV--SGELAP 113
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 122 VYQIANN--CPQLMKIVAMKANMHLRDLPnacyweDFLNVVPNEAEFEKRLNSKQLSDLFTLiYTSGTTGEPKGVMLDYA 199
Cdd:cd05959  114 VLAAALTksEHTLVVLIVSGGAGPEAGAL------LLAELVAAEAEQLKPAATHADDPAFWL-YSSGSTGRPKGVVHLHA 186
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 200 NLAHQLN--AHDLaLNVNEDDVSLSFLPLSHiferawiAY--------VFHRGATnCYLE----DTKHVRDALTTLKPTV 265
Cdd:cd05959  187 DIYWTAElyARNV-LGIREDDVCFSAAKLFF-------AYglgnsltfPLSVGAT-TVLMperpTPAAVFKRIRRYRPTV 257
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 266 MCAVPRFYekiytAVWDKVEKAPlhrralfnwairvgeqhyqieqpsqwlrlQYALAdklvltklrallggRIKMMPCGG 345
Cdd:cd05959  258 FFGVPTLY-----AAMLAAPNLP-----------------------------SRDLS--------------SLRLCVSAG 289
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 346 AKLEASIGSFFHS-IGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI----------GEENEILVRGGMVM 414
Cdd:cd05959  290 EALPAEVGERWKArFGLDILDGIGSTEMLHIFLSNRPGRVRYGTTGKPVPGYEVELrdedggdvadGEPGELYVRGPSSA 369
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 415 RGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVIA----DA 490
Cdd:cd05959  370 TMYWNNRDKTRDTF-QGEWTRTGDKYVRDDDGFYTYAGRADDMLKVS-GIWVSPFEVESALVQHPAVLEAAVVGvedeDG 447
                        490       500       510
                 ....*....|....*....|....*....|
gi 544685990 491 KKYVSALIVP---------CFDSLEEYAKQ 511
Cdd:cd05959  448 LTKPKAFVVLrpgyedseaLEEELKEFVKD 477
A_NRPS_VisG_like cd17651
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ...
46-489 1.58e-19

similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341306 [Multi-domain]  Cd Length: 491  Bit Score: 92.02  E-value: 1.58e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  46 DRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQEQFDQvyqi 125
Cdd:cd17651   31 NRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYPAERLAFMLADAGPVLVLTHPALAGEL---- 106
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 126 anncpqlmkivamkanmhLRDLPNACYWEDFLNVVPNEAEFEKRLnskQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQL 205
Cdd:cd17651  107 ------------------AVELVAVTLLDQPGAAAGADAEPDPAL---DADDLAYVIYTSGSTGRPKGVVMPHRSLANLV 165
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 206 NAHDLALNVNEDDVSLSFLPLSH--IFERAWIAYVFhrGATNCYLEDtkHVRDALTTLkptvmcavprfyekiytavWDK 283
Cdd:cd17651  166 AWQARASSLGPGARTLQFAGLGFdvSVQEIFSTLCA--GATLVLPPE--EVRTDPPAL-------------------AAW 222
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 284 VEKAPLHRRALFNWAIR-VGEQHYQIEQPSQWLRLQYALADKLVLTK-LRALLGGRikmmpcGGAKLeasigsFFHsigi 361
Cdd:cd17651  223 LDEQRISRVFLPTVALRaLAEHGRPLGVRLAALRYLLTGGEQLVLTEdLREFCAGL------PGLRL------HNH---- 286
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 362 niklgYGMTETTaTVSCWQDKGFN-----PNSIGTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAK 426
Cdd:cd17651  287 -----YGPTETH-VVTALSLPGDPaawpaPPPIGRPIDNTRVYVldaalrpvppGVPGELYIGGAGLARGYLNRPELTAE 360
                        410       420       430       440       450       460
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 544685990 427 AFTEDGFL------RTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGKIGKDKFIEQIAVIAD 489
Cdd:cd17651  361 RFVPDPFVpgarmyRTGDLARWLPDGELEFLGRADDQVKI-RGFRIELGEIEAALARHPGVREAVVLAR 428
FACL_like_4 cd05944
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
176-472 2.07e-19

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341266 [Multi-domain]  Cd Length: 359  Bit Score: 90.23  E-value: 2.07e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 176 SDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFErawiAYV-----FHRGATncyled 250
Cdd:cd05944    2 DDVAAYFHTGGTTGTPKLAQHTHSNEVYNAWMLALNSLFDPDDVLLCGLPLFHVNG----SVVtlltpLASGAH------ 71
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 251 tkhvrdalttlkptVMCAVPRFY--EKIYTAVWDKVEkaplhrralfnwairvgeqHYQIEQPSQwlrlqyaladklVLT 328
Cdd:cd05944   72 --------------VVLAGPAGYrnPGLFDNFWKLVE-------------------RYRITSLST------------VPT 106
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 KLRALLG-------GRIKMMPCGGAKLEASIGSFFH-SIGINIKLGYGMTETTATVSC-WQDKGFNPNSIGTLMPNAEVK 399
Cdd:cd05944  107 VYAALLQvpvnadiSSLRFAMSGAAPLPVELRARFEdATGLPVVEGYGLTEATCLVAVnPPDGPKRPGSVGLRLPYARVR 186
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 400 I---------------GEENEILVRGGMVMRGYYKKpEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGK 464
Cdd:cd05944  187 IkvldgvgrllrdcapDEVGEICVAGPGVFGGYLYT-EGNKNAFVADGWLNTGDLGRLDADGYLFITGRAKDLI-IRGGH 264

                 ....*...
gi 544685990 465 YIAPQYIE 472
Cdd:cd05944  265 NIDPALIE 272
PLN02860 PLN02860
o-succinylbenzoate-CoA ligase
183-577 2.17e-19

o-succinylbenzoate-CoA ligase


Pssm-ID: 215464 [Multi-domain]  Cd Length: 563  Bit Score: 91.78  E-value: 2.17e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 183 YTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLE--DTKHVRDALTT 260
Cdd:PLN02860 179 FTSGTTGRPKGVTISHSALIVQSLAKIAIVGYGEDDVYLHTAPLCHIGGLSSALAMLMVGACHVLLPkfDAKAALQAIKQ 258
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 261 LKPTVMCAVPrfyekiyTAVWDKVEKAplhrRALFNWAIRvgeqhyqieqpsqwlrlqyaladKLVLTKLRA-------L 333
Cdd:PLN02860 259 HNVTSMITVP-------AMMADLISLT----RKSMTWKVF-----------------------PSVRKILNGggslssrL 304
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 334 LGGRIKMMPCggakleASIGSffhsiginiklGYGMTETTATVS--------------CWQDKGFNPNS---------IG 390
Cdd:PLN02860 305 LPDAKKLFPN------AKLFS-----------AYGMTEACSSLTfmtlhdptlespkqTLQTVNQTKSSsvhqpqgvcVG 367
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 391 TLMPNAEVKIG-----EENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKY 465
Cdd:PLN02860 368 KPAPHVELKIGldessRVGRILTRGPHVMLGYWGQNSETASVLSNDGWLDTGDIGWIDKAGNLWLIGRSNDRIKTG-GEN 446
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 466 IAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFdSLEEYAKQLNIKYQDRieliKHSDIIQMFERRIHELQKELPS 545
Cdd:PLN02860 447 VYPEEVEAVLSQHPGVASVVVVGVPDSRLTEMVVACV-RLRDGWIWSDNEKENA----KKNLTLSSETLRHHCREKNLSR 521
                        410       420       430
                 ....*....|....*....|....*....|..
gi 544685990 546 FEQVKRFTLLPQAFSTameeiTPTLKLRRKVI 577
Cdd:PLN02860 522 FKIPKLFVQWRKPFPL-----TTTGKIRRDEV 548
PRK05691 PRK05691
peptide synthase; Validated
21-472 9.50e-19

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 91.00  E-value: 9.50e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   21 NRTALRF--REQAQWQEMSWQTFQQEIdRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQ 98
Cdd:PRK05691   24 DRLALRFlaDDPGEGVVLSYRDLDLRA-RTIAAALQARASFGDRAVLLFPSGPDYVAAFFGCLYAGVIAVPAYPPESARR 102
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   99 -----VEYIVNDADIKILFVGD--QEQFDQVYQI-ANNCPQLMKIvamkanmhlrdlpnacyweDFLNVVPNEAEFEKRL 170
Cdd:PRK05691  103 hhqerLLSIIADAEPRLLLTVAdlRDSLLQMEELaAANAPELLCV-------------------DTLDPALAEAWQEPAL 163
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  171 nskQLSDLFTLIYTSGTTGEPKGVMLDYANLA--HQLNAHDLALNVNEDDVSLSFLPLSH-----------IFerAWIAY 237
Cdd:PRK05691  164 ---QPDDIAFLQYTSGSTALPKGVQVSHGNLVanEQLIRHGFGIDLNPDDVIVSWLPLYHdmgliggllqpIF--SGVPC 238
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  238 VFHrgATNCYLEDTKHVRDALTTLKPTVMCAvPRFyekIYTAVWDKVEKAPLHRRALFNWairvgeqhyqieqpsqwlRL 317
Cdd:PRK05691  239 VLM--SPAYFLERPLRWLEAISEYGGTISGG-PDF---AYRLCSERVSESALERLDLSRW------------------RV 294
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  318 QYALADKLVLTKLRALLGgriKMMPCGgakLEASigSFFHSiginiklgYGMTETTATVSCWQ-----------DKGFNP 386
Cdd:PRK05691  295 AYSGSEPIRQDSLERFAE---KFAACG---FDPD--SFFAS--------YGLAEATLFVSGGRrgqgipaleldAEALAR 358
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  387 N-----------SIGTLMPNAEVKIGEEN-----------EILVRGGMVMRGYYKKPEETAKAFTE-DG--FLRTGDVGE 441
Cdd:PRK05691  359 NraepgtgsvlmSCGRSQPGHAVLIVDPQslevlgdnrvgEIWASGPSIAHGYWRNPEASAKTFVEhDGrtWLRTGDLGF 438
                         490       500       510
                  ....*....|....*....|....*....|.
gi 544685990  442 MDScGNLFITDRLKElMKTSNGKYIAPQYIE 472
Cdd:PRK05691  439 LRD-GELFVTGRLKD-MLIVRGHNLYPQDIE 467
PLN02574 PLN02574
4-coumarate--CoA ligase-like
177-488 9.66e-19

4-coumarate--CoA ligase-like


Pssm-ID: 215312 [Multi-domain]  Cd Length: 560  Bit Score: 89.90  E-value: 9.66e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANLAHQLNAH-----DLALNVNEDDVSLSFLPLSHIFERAwiayVFHRGatncyledt 251
Cdd:PLN02574 199 DVAAIMYSSGTTGASKGVVLTHRNLIAMVELFvrfeaSQYEYPGSDNVYLAALPMFHIYGLS----LFVVG--------- 265
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 252 khvrdaLTTLKPTVMcAVPRFYEKIYTAVWDkvekaplhrralfnwaiRVGEQHYQIEQPsqwlrlqyaladklVLTKL- 330
Cdd:PLN02574 266 ------LLSLGSTIV-VMRRFDASDMVKVID-----------------RFKVTHFPVVPP--------------ILMALt 307
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 331 ---RALLGG---RIKMMPCGGAKL-EASIGSFFHSIG-INIKLGYGMTETTATVScwqdKGFNP------NSIGTLMPNA 396
Cdd:PLN02574 308 kkaKGVCGEvlkSLKQVSCGAAPLsGKFIQDFVQTLPhVDFIQGYGMTESTAVGT----RGFNTeklskySSVGLLAPNM 383
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 397 EVKI-----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTsNGKY 465
Cdd:PLN02574 384 QAKVvdwstgcllppGNCGELWIQGPGVMKGYLNNPKATQSTIDKDGWLRTGDIAYFDEDGYLYIVDRLKEIIKY-KGFQ 462
                        330       340
                 ....*....|....*....|...
gi 544685990 466 IAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:PLN02574 463 IAPADLEAVLISHPEIIDAAVTA 485
PRK05852 PRK05852
fatty acid--CoA ligase family protein;
367-500 1.15e-18

fatty acid--CoA ligase family protein;


Pssm-ID: 235625 [Multi-domain]  Cd Length: 534  Bit Score: 89.56  E-value: 1.15e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 367 YGMTETTATVSC----WQDKGFNPN-SIGTLMPNAEVKI------------GEENEILVRGGMVMRGYYKKPEETAKAFT 429
Cdd:PRK05852 327 FGMTEATHQVTTtqieGIGQTENPVvSTGLVGRSTGAQIrivgsdglplpaGAVGEVWLRGTTVVRGYLGDPTITAANFT 406
                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 430 eDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVIADAKKY----VSALIVP 500
Cdd:PRK05852 407 -DGWLRTGDLGSLSAAGDLSIRGRIKELINRG-GEKISPERVEGVLASHPNVMEAAVFGVPDQLygeaVAAVIVP 479
PRK08162 PRK08162
acyl-CoA synthetase; Validated
37-491 1.74e-18

acyl-CoA synthetase; Validated


Pssm-ID: 236169 [Multi-domain]  Cd Length: 545  Bit Score: 88.85  E-value: 1.74e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  37 SWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVavpIYATNT---AKQVEYIVNDADIKILFV 113
Cdd:PRK08162  45 TWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAV---LNTLNTrldAASIAFMLRHGEAKVLIV 121
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 114 gDQEQFDQVYQIANNCPQLMKIV------AMKANMHLRDLPnacyWEDFLNvvPNEAEFEKRLNSKQLsDLFTLIYTSGT 187
Cdd:PRK08162 122 -DTEFAEVAREALALLPGPKPLVidvddpEYPGGRFIGALD----YEAFLA--SGDPDFAWTLPADEW-DAIALNYTSGT 193
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 188 TGEPKGVMldYANLAHQLNA--HDLALNVNEDDVSLSFLPLSHI----FerAWIayVFHRGATNCYLE--DTKHVRDALT 259
Cdd:PRK08162 194 TGNPKGVV--YHHRGAYLNAlsNILAWGMPKHPVYLWTLPMFHCngwcF--PWT--VAARAGTNVCLRkvDPKLIFDLIR 267
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 260 TLKPTVMCAvprfyekiytavwdkvekAPLHRRALFNwairvgeqhyqieQPSQWlrlqyaladklvltklRALLGGRIK 339
Cdd:PRK08162 268 EHGVTHYCG------------------APIVLSALIN-------------APAEW----------------RAGIDHPVH 300
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 340 MMPCGGAKLEASIGSFfHSIGINIKLGYGMTET--TATVSCWQD-------------KG--------------FNPNSiG 390
Cdd:PRK08162 301 AMVAGAAPPAAVIAKM-EEIGFDLTHVYGLTETygPATVCAWQPewdalplderaqlKArqgvryplqegvtvLDPDT-M 378
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 391 TLMPNAEVKIGEeneILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQY 470
Cdd:PRK08162 379 QPVPADGETIGE---IMFRGNIVMKGYLKNPKATEEAF-AGGWFHTGDLAVLHPDGYIKIKDRSKDII-ISGGENISSIE 453
                        490       500
                 ....*....|....*....|...
gi 544685990 471 IEGKIGKDKFIEQIAVIA--DAK 491
Cdd:PRK08162 454 VEDVLYRHPAVLVAAVVAkpDPK 476
MACS_like cd05972
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ...
176-488 3.19e-18

Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.


Pssm-ID: 341276 [Multi-domain]  Cd Length: 428  Bit Score: 87.39  E-value: 3.19e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 176 SDLFTLIYTSGTTGEPKGVMLDY-ANLAHQLNAHDlALNVNEDDVSLSflplshIFERAWIAYVFHR-------GATN-- 245
Cdd:cd05972   81 EDPALIYFTSGTTGLPKGVLHTHsYPLGHIPTAAY-WLGLRPDDIHWN------IADPGWAKGAWSSffgpwllGATVfv 153
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 246 CYLE--DTKHVRDALTTLKPTVMCAVPrfyekiytAVWDKVEKAPLHRRALfnwairvgeqhyqieqpsqwLRLQYALad 323
Cdd:cd05972  154 YEGPrfDAERILELLERYGVTSFCGPP--------TAYRMLIKQDLSSYKF--------------------SHLRLVV-- 203
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 324 klvltklrallggrikmmpCGGAKL-EASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI-- 400
Cdd:cd05972  204 -------------------SAGEPLnPEVIEWWRAATGLPIRDGYGQTETGLTVGNFPDMPVKPGSMGRPTPGYDVAIid 264
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 --------GEENEILVRGGMV--MRGYYKKPEETAKAFTEDgFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQY 470
Cdd:cd05972  265 ddgrelppGEEGDIAIKLPPPglFLGYVGDPEKTEASIRGD-YYLTGDRAYRDEDGYFWFVGRADDIIKSS-GYRIGPFE 342
                        330
                 ....*....|....*...
gi 544685990 471 IEGKIGKDKFIEQIAVIA 488
Cdd:cd05972  343 VESALLEHPAVAEAAVVG 360
A_NRPS_LgrA-like cd17645
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ...
12-500 7.38e-18

adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.


Pssm-ID: 341300 [Multi-domain]  Cd Length: 440  Bit Score: 86.45  E-value: 7.38e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  12 FRLQAKKWLNRTALRFREQAqwqeMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:cd17645    4 FEEQVERTPDHVAVVDRGQS----LTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPID 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  92 ATNTAKQVEYIVNDADIKILFvgdqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneaefekrln 171
Cdd:cd17645   80 PDYPGERIAYMLADSSAKILL----------------------------------------------------------- 100
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 172 sKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLShiFErAWIAYVFhrgaTNCYLEDT 251
Cdd:cd17645  101 -TNPDDLAYVIYTSGSTGLPKGVMIEHHNLVNLCEWHRPYFGVTPADKSLVYASFS--FD-ASAWEIF----PHLTAGAA 172
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 252 KHVRDALTTLKptvMCAVPRFYEKiytavwdkvekaplHRRALFNWAIRVGEQHYQIEQPSqwlrlqyaladklvltkLR 331
Cdd:cd17645  173 LHVVPSERRLD---LDALNDYFNQ--------------EGITISFLPTGAAEQFMQLDNQS-----------------LR 218
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 332 ALLggrikmmpCGGAKLEAsigsfFHSIGINIKLGYGMTETTATVSCWQ-DKGFNPNSIGTLMPNAEVKI---------- 400
Cdd:cd17645  219 VLL--------TGGDKLKK-----IERKGYKLVNNYGPTENTVVATSFEiDKPYANIPIGKPIDNTRVYIldealqlqpi 285
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFL------RTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGK 474
Cdd:cd17645  286 GVAGELCIAGEGLARGYLNRPELTAEKFIVHPFVpgermyRTGDLAKFLPDGNIEFLGRLDQQVKI-RGYRIEPGEIEPF 364
                        490       500       510
                 ....*....|....*....|....*....|
gi 544685990 475 IGKDKFIEQIAVIA----DAKKYVSALIVP 500
Cdd:cd17645  365 LMNHPLIELAAVLAkedaDGRKYLVAYVTA 394
A_NRPS_GliP_like cd17653
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ...
43-459 3.28e-17

nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341308 [Multi-domain]  Cd Length: 433  Bit Score: 84.28  E-value: 3.28e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  43 QEIDRFSYAL---IAQHIDIQ-DKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILfvgdqeq 118
Cdd:cd17653   26 GELDAASNALanrLLQLGVVPgDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAKLPSARIQAILRTSGATLL------- 98
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 119 fdqvyqIANNCPQlmkivamkanmhlrdlpnacywedflnvvpneaefekrlnskqlsDLFTLIYTSGTTGEPKGVMLDY 198
Cdd:cd17653   99 ------LTTDSPD---------------------------------------------DLAYIIFTSGSTGIPKGVMVPH 127
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 199 ANLAHQLNAHDLALNVNEDDVSLSFLPLShiFErAWIAYVFH---RGATNCYLEDTKHVRDALTTLkpTVMCAVPRFYEK 275
Cdd:cd17653  128 RGVLNYVSQPPARLDVGPGSRVAQVLSIA--FD-ACIGEIFStlcNGGTLVLADPSDPFAHVARTV--DALMSTPSILST 202
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 276 IYTAVWDKVEKaplhrralfnwaIRVGEqhyqiEQPSQWLrlqyaladklvltkLRALLGGRikmmpcggakleasigSF 355
Cdd:cd17653  203 LSPQDFPNLKT------------IFLGG-----EAVPPSL--------------LDRWSPGR----------------RL 235
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 356 FHsiginiklGYGMTETTATVSCWQDKGFNPNSIGTLMPNA----------EVKIGEENEILVRGGMVMRGYYKKPEETA 425
Cdd:cd17653  236 YN--------AYGPTECTISSTMTELLPGQPVTIGKPIPNStcyildadlqPVPEGVVGEICISGVQVARGYLGNPALTA 307
                        410       420       430       440
                 ....*....|....*....|....*....|....*....|
gi 544685990 426 KAFTEDGF------LRTGDVGEMDSCGNLFITDRLKELMK 459
Cdd:cd17653  308 SKFVPDPFwpgsrmYRTGDYGRWTEDGGLEFLGREDNQVK 347
PRK07787 PRK07787
acyl-CoA synthetase; Validated
182-453 7.28e-17

acyl-CoA synthetase; Validated


Pssm-ID: 236096 [Multi-domain]  Cd Length: 471  Bit Score: 83.50  E-value: 7.28e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 182 IYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIferawiayvfH-----------RGATNCYL-- 248
Cdd:PRK07787 134 VYTSGTTGPPKGVVLSRRAIAADLDALAEAWQWTADDVLVHGLPLFHV----------HglvlgvlgplrIGNRFVHTgr 203
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 249 EDTKHVRDALTTlKPTVMCAVPrfyekiytAVWDKVEKAPLHRRALfnwairvgeqhyqieQPSQWLRLQYALADKLVLT 328
Cdd:PRK07787 204 PTPEAYAQALSE-GGTLYFGVP--------TVWSRIAADPEAARAL---------------RGARLLVSGSAALPVPVFD 259
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 KLRALLGGRIkmmpcggakLEAsigsffhsiginiklgYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKIGEEN---- 404
Cdd:PRK07787 260 RLAALTGHRP---------VER----------------YGMTETLITLSTRADGERRPGWVGLPLAGVETRLVDEDggpv 314
                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990 405 --------EILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDR 453
Cdd:PRK07787 315 phdgetvgELQVRGPTLFDGYLNRPDATAAAFTADGWFRTGDVAVVDPDGMHRIVGR 371
PRK07008 PRK07008
long-chain-fatty-acid--CoA ligase; Validated
51-472 2.33e-16

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 235908 [Multi-domain]  Cd Length: 539  Bit Score: 82.06  E-value: 2.33e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  51 ALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFvgdqeqFDQ-----VYQI 125
Cdd:PRK07008  55 ALAALGVEPGDRVGTLAWNGYRHLEAYYGVSGSGAVCHTINPRLFPEQIAYIVNHAEDRYVL------FDLtflplVDAL 128
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 126 ANNCPQLMKIVAM--KANMHLRDLPNACYwEDFLNVVPNEAEFeKRLNSKQLSdlfTLIYTSGTTGEPKGVMldYANLAH 203
Cdd:PRK07008 129 APQCPNVKGWVAMtdAAHLPAGSTPLLCY-ETLVGAQDGDYDW-PRFDENQAS---SLCYTSGTTGNPKGAL--YSHRST 201
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 204 QLNAHDLAL----NVNEDDVSLSFLPLSHIfeRAW-IAYvfhrgatNCYLEDTKHVrdalttlkptvmcavprFYEKiyt 278
Cdd:PRK07008 202 VLHAYGAALpdamGLSARDAVLPVVPMFHV--NAWgLPY-------SAPLTGAKLV-----------------LPGP--- 252
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 279 avwdKVEKAPLHRraLFNwAIRVGeqhYQIEQPSQWLR-LQYALADKLVLTKLRALLGGrikmmpcGGAKLEASIGSFFH 357
Cdd:PRK07008 253 ----DLDGKSLYE--LIE-AERVT---FSAGVPTVWLGlLNHMREAGLRFSTLRRTVIG-------GSACPPAMIRTFED 315
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 358 SIGINIKLGYGMTE-----TTATVScWQDKGFNPNSI-------GTLMPNAEVKI----GEE--------NEILVRGGMV 413
Cdd:PRK07008 316 EYGVEVIHAWGMTEmsplgTLCKLK-WKHSQLPLDEQrkllekqGRVIYGVDMKIvgddGRElpwdgkafGDLQVRGPWV 394
                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 544685990 414 MRGYYKKPEETakafTEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNGKYIAPQYIE 472
Cdd:PRK07008 395 IDRYFRGDASP----LVDGWFPTGDVATIDADGFMQITDRSKDVIK-SGGEWISSIDIE 448
A_NRPS_Srf_like cd12117
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ...
11-448 3.09e-16

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.


Pssm-ID: 341282 [Multi-domain]  Cd Length: 483  Bit Score: 81.48  E-value: 3.09e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  11 RFRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI 90
Cdd:cd12117    2 LFEEQAARTPDAVAVVYGDR----SLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPL 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  91 YATNTAKQVEYIVNDADIKILfVGDQEQFDQVyqiANNCPQLMKIVAMKAnmhlRDLPNAcywedflnVVPNEAEfekrl 170
Cdd:cd12117   78 DPELPAERLAFMLADAGAKVL-LTDRSLAGRA---GGLEVAVVIDEALDA----GPAGNP--------AVPVSPD----- 136
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 171 nskqlsDLFTLIYTSGTTGEPKGVMLDYANLAhqlnahDLALNVN-----EDDVSLSFLPLShiFERA----WIAYVFhr 241
Cdd:cd12117  137 ------DLAYVMYTSGSTGRPKGVAVTHRGVV------RLVKNTNyvtlgPDDRVLQTSPLA--FDAStfeiWGALLN-- 200
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 242 GATnCYLEDTKHVRD------ALTTLKPTVMCAvprfyekiyTAvwdkvekaplhrrALFNwairvgeqhyqieqpsqwl 315
Cdd:cd12117  201 GAR-LVLAPKGTLLDpdalgaLIAEEGVTVLWL---------TA-------------ALFN------------------- 238
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 316 rlQYALADKLVLTKLRALL-GG--------RIKMMPCGGAKLeasigsffhsigINiklGYGMTETTaTVSCW---QDKG 383
Cdd:cd12117  239 --QLADEDPECFAGLRELLtGGevvspphvRRVLAACPGLRL------------VN---GYGPTENT-TFTTShvvTELD 300
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 384 FNPNS--IGTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFL------RTGDVGEMDSC 445
Cdd:cd12117  301 EVAGSipIGRPIANTRVYVldedgrpvppGVPGELYVGGDGLALGYLNRPALTAERFVADPFGpgerlyRTGDLARWLPD 380

                 ...
gi 544685990 446 GNL 448
Cdd:cd12117  381 GRL 383
PLN03102 PLN03102
acyl-activating enzyme; Provisional
21-488 3.48e-16

acyl-activating enzyme; Provisional


Pssm-ID: 215576 [Multi-domain]  Cd Length: 579  Bit Score: 81.99  E-value: 3.48e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:PLN03102  29 NRTSIIYGKT----RFTWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIA 104
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFVgdQEQFDQVYQianncPQLMKIVAMKANMHLRdlpnacywedflNVVPNEAEFEKRLNSKQLS---- 176
Cdd:PLN03102 105 AILRHAKPKILFV--DRSFEPLAR-----EVLHLLSSEDSNLNLP------------VIFIHEIDFPKRPSSEELDyecl 165
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 ---------------------DLFTLIYTSGTTGEPKGVMLDyanlahqlnahdlalnvneddvslsflplshiferawi 235
Cdd:PLN03102 166 iqrgeptpslvarmfriqdehDPISLNYTSGTTADPKGVVIS-------------------------------------- 207
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 236 ayvfHRGAtncYLEDTKHVRDALTTLKPTVMCAVPRFYEKIYTAVWDKVEkaplhrRALFNWAIRvgeqHYQIEQPSQWL 315
Cdd:PLN03102 208 ----HRGA---YLSTLSAIIGWEMGTCPVYLWTLPMFHCNGWTFTWGTAA------RGGTSVCMR----HVTAPEIYKNI 270
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 316 RLQYALADKLVLTKLRALL-GGRIKMMP--------CGGAKLEASIGSFFHSIGINIKLGYGMTETTATV-SC-WQD--- 381
Cdd:PLN03102 271 EMHNVTHMCCVPTVFNILLkGNSLDLSPrsgpvhvlTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVlFCeWQDewn 350
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 382 ----------KGFNPNSIGTLMpNAEVKIGEE-----------NEILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVG 440
Cdd:PLN03102 351 rlpenqqmelKARQGVSILGLA-DVDVKNKETqesvprdgktmGEIVIKGSSIMKGYLKNPKATSEAF-KHGWLNTGDVG 428
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*...
gi 544685990 441 EMDSCGNLFITDRLKELMkTSNGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:PLN03102 429 VIHPDGHVEIKDRSKDII-ISGGENISSVEVENVLYKYPKVLETAVVA 475
PRK13295 PRK13295
cyclohexanecarboxylate-CoA ligase; Reviewed
23-500 2.65e-15

cyclohexanecarboxylate-CoA ligase; Reviewed


Pssm-ID: 171961 [Multi-domain]  Cd Length: 547  Bit Score: 78.94  E-value: 2.65e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  23 TALRfREQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYI 102
Cdd:PRK13295  44 TAVR-LGTGAPRRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAVLNPLMPIFRERELSFM 122
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 103 VNDADIKILFVG------DQEQFdqVYQIANNCPQLMKIVAMKAnmhlrDLPNAcyWEDFLNvvpnEAEFEKRLNSKQLS 176
Cdd:PRK13295 123 LKHAESKVLVVPktfrgfDHAAM--ARRLRPELPALRHVVVVGG-----DGADS--FEALLI----TPAWEQEPDAPAIL 189
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 --------DLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFErawiayvFHRGAtncyl 248
Cdd:PRK13295 190 arlrpgpdDVTQLIYTSGTTGEPKGVMHTANTLMANIVPYAERLGLGADDVILMASPMAHQTG-------FMYGL----- 257
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 249 edtkhvrdalttLKPTVMCAvprfyEKIYTAVWDKVEKAPLHRRALFNWAIRvgeqhyqiEQPsqwlrlqyALADKLVLT 328
Cdd:PRK13295 258 ------------MMPVMLGA-----TAVLQDIWDPARAAELIRTEGVTFTMA--------STP--------FLTDLTRAV 304
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 KLRALLGGRIKMMPCGGAKLE-ASIGSFFHSIGINIKLGYGMTETTA-TVSCWQD---KGFNpnSIGTLMPNAEVKI--- 400
Cdd:PRK13295 305 KESGRPVSSLRTFLCAGAPIPgALVERARAALGAKIVSAWGMTENGAvTLTKLDDpdeRAST--TDGCPLPGVEVRVvda 382
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -------GEENEILVRGGMVMRGYYKKPEETAKAFteDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEG 473
Cdd:PRK13295 383 dgaplpaGQIGRLQVRGCSNFGGYLKRPQLNGTDA--DGWFDTGDLARIDADGYIRISGRSKDVI-IRGGENIPVVEIEA 459
                        490       500       510
                 ....*....|....*....|....*....|.
gi 544685990 474 KIGKDKFIEQIAVIA--DAK--KYVSALIVP 500
Cdd:PRK13295 460 LLYRHPAIAQVAIVAypDERlgERACAFVVP 490
PRK12406 PRK12406
long-chain-fatty-acid--CoA ligase; Provisional
36-472 3.20e-15

long-chain-fatty-acid--CoA ligase; Provisional


Pssm-ID: 183506 [Multi-domain]  Cd Length: 509  Bit Score: 78.59  E-value: 3.20e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  36 MSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVGD 115
Cdd:PRK12406  12 RSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLIAHA 91
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 116 qeqfDQVYQIANNCPQLMKIV----------AMKANMHLRDLP-NACYWEDFLnvvpneAEFEKrLNSKQLSDLFTLIYT 184
Cdd:PRK12406  92 ----DLLHGLASALPAGVTVLsvptppeiaaAYRISPALLTPPaGAIDWEGWL------AQQEP-YDGPPVPQPQSMIYT 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 185 SGTTGEPKGVMLDYANLAHQLNAHD---LALNVNEDDVSLSFLPLSHiferawiayvfhrGATNCYledtkhvrdALTTL 261
Cdd:PRK12406 161 SGTTGHPKGVRRAAPTPEQAAAAEQmraLIYGLKPGIRALLTGPLYH-------------SAPNAY---------GLRAG 218
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 262 K-PTVMCAVPRFYEKiytAVWDKVEKAplhrralfnwaiRVGEQHYQIEQPSQWLRLQYALADKLVLTKLRALLGGrikM 340
Cdd:PRK12406 219 RlGGVLVLQPRFDPE---ELLQLIERH------------RITHMHMVPTMFIRLLKLPEEVRAKYDVSSLRHVIHA---A 280
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 341 MPCGGAKLEASIGSFfhsiGINIKLGYGMTETTATVSCWQDKGFN-PNSIGTLMPNAEVKI----------GEENEILVR 409
Cdd:PRK12406 281 APCPADVKRAMIEWW----GPVIYEYYGSTESGAVTFATSEDALShPGTVGKAAPGAELRFvdedgrplpqGEIGEIYSR 356
                        410       420       430       440       450       460
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 544685990 410 -GGMVMRGYYKKPEETAKAfTEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIE 472
Cdd:PRK12406 357 iAGNPDFTYHNKPEKRAEI-DRGGFITSGDVGYLDADGYLFLCDRKRD-MVISGGVNIYPAEIE 418
23DHB-AMP_lg cd05920
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ...
397-525 5.11e-15

2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.


Pssm-ID: 341244 [Multi-domain]  Cd Length: 482  Bit Score: 77.75  E-value: 5.11e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 397 EVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEGKIG 476
Cdd:cd05920  329 PVPPGEEGELLTRGPYTIRGYYRAPEHNARAFTPDGFYRTGDLVRRTPDGYLVVEGRIKDQI-NRGGEKIAAEEVENLLL 407
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 544685990 477 KDKFIEQIAVIADAKKY----VSALIV-----PCFDSLEEYAKQLNI---KYQDRIELIKH 525
Cdd:cd05920  408 RHPAVHDAAVVAMPDELlgerSCAFVVlrdppPSAAQLRRFLRERGLaayKLPDRIEFVDS 468
PRK06155 PRK06155
crotonobetaine/carnitine-CoA ligase; Provisional
13-488 5.56e-15

crotonobetaine/carnitine-CoA ligase; Provisional


Pssm-ID: 235719 [Multi-domain]  Cd Length: 542  Bit Score: 77.88  E-value: 5.56e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  13 RLQAKKWLNRTALRFREQaqwqemsWQTFQQEIDRFS---YALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVP 89
Cdd:PRK06155  28 ARQAERYPDRPLLVFGGT-------RWTYAEAARAAAaaaHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVP 100
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  90 IyatNTA---KQVEYIVNDADIKiLFVGDQEQFDQVYQIANNCPQLMKIVAMkanmhlrDLPNACYWEDFLNVVPNEAEF 166
Cdd:PRK06155 101 I---NTAlrgPQLEHILRNSGAR-LLVVEAALLAALEAADPGDLPLPAVWLL-------DAPASVSVPAGWSTAPLPPLD 169
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 167 EK-RLNSKQLSDLFTLIYTSGTTGEPKGVMLDYA-------NLAHQLNahdlalnVNEDDVSLSFLPLshiferawiayv 238
Cdd:PRK06155 170 APaPAAAVQPGDTAAILYTSGTTGPSKGVCCPHAqfywwgrNSAEDLE-------IGADDVLYTTLPL------------ 230
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 239 FHRGATNCYLEdtkhvrdALttLKPTVMCAVPRFYEKIYtavWDKVEKAplhrRALFNWAIrvGEQ-HYQIEQPSqwlrl 317
Cdd:PRK06155 231 FHTNALNAFFQ-------AL--LAGATYVLEPRFSASGF---WPAVRRH----GATVTYLL--GAMvSILLSQPA----- 287
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 318 qyALADKLvlTKLRALLGgrikmmPCGGAKLEASigsFFHSIGINIKLGYGMTETTATVSC-WQDKgfNPNSIGTLMPNA 396
Cdd:PRK06155 288 --RESDRA--HRVRVALG------PGVPAALHAA---FRERFGVDLLDGYGSTETNFVIAVtHGSQ--RPGSMGRLAPGF 352
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 397 EVKIGEEN----------EILVRG---GMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNG 463
Cdd:PRK06155 353 EARVVDEHdqelpdgepgELLLRAdepFAFATGYFGMPEKTVEAW-RNLWFHTGDRVVRDADGWFRFVDRIKDAIR-RRG 430
                        490       500
                 ....*....|....*....|....*
gi 544685990 464 KYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:PRK06155 431 ENISSFEVEQVLLSHPAVAAAAVFP 455
PRK12316 PRK12316
peptide synthase; Provisional
11-439 6.73e-15

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 78.85  E-value: 6.73e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   11 RFRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI 90
Cdd:PRK12316 4556 LVAERARMTPDAVAVVFDEE----KLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGAYVPL 4631
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   91 YATNTAKQVEYIVNDADIKILFVgdQEQFDQVYQIAnncpqlmkivamkANMHLRDLPNACYWEDFlnvvpNEAEFEKRL 170
Cdd:PRK12316 4632 DPEYPRERLAYMMEDSGAALLLT--QSHLLQRLPIP-------------DGLASLALDRDEDWEGF-----PAHDPAVRL 4691
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  171 NSKQLSdlfTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS-HIFERAWIaYVFHRGATncyle 249
Cdd:PRK12316 4692 HPDNLA---YVIYTSGSTGRPKGVAVSHGSLVNHLHATGERYELTPDDRVLQFMSFSfDGSHEGLY-HPLINGAS----- 4762
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  250 dtKHVRDAlttlkptvmcavprfyekiytAVWDkvekaPLHRRALfnwairVGEQHYQIEQ--PSQWLRL-QYALADKLV 326
Cdd:PRK12316 4763 --VVIRDD---------------------SLWD-----PERLYAE------IHEHRVTVLVfpPVYLQQLaEHAERDGEP 4808
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  327 LTKLRALLGGRikmmPCGGAKLEASIGSFFHSIGINiklGYGMTETTATVSCWQDKGFNPNS-----IGTLMPN------ 395
Cdd:PRK12316 4809 PSLRVYCFGGE----AVAQASYDLAWRALKPVYLFN---GYGPTETTVTVLLWKARDGDACGaaympIGTPLGNrsgyvl 4881
                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 544685990  396 ----AEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGDV 439
Cdd:PRK12316 4882 dgqlNPLPVGVAGELYLGGEGVARGYLERPALTAERFVPDPFgapggrlYRTGDL 4936
PRK07798 PRK07798
acyl-CoA synthetase; Validated
21-195 8.24e-15

acyl-CoA synthetase; Validated


Pssm-ID: 236100 [Multi-domain]  Cd Length: 533  Bit Score: 77.23  E-value: 8.24e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:PRK07798  18 DRVALVCGDR----RLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRYVEDELR 93
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFVGDqeQF-DQVYQIANNCPQLMKIVAMKANMHLRDLPNACYWEDFLNVVPNEAEFEKRlnskqlS--D 177
Cdd:PRK07798  94 YLLDDSDAVALVYER--EFaPRVAEVLPRLPKLRTLVVVEDGSGNDLLPGAVDYEDALAAGSPERDFGER------SpdD 165
                        170
                 ....*....|....*...
gi 544685990 178 LFtLIYTSGTTGEPKGVM 195
Cdd:PRK07798 166 LY-LLYTGGTTGMPKGVM 182
EntE COG1021
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ...
397-488 1.44e-14

EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 440644 [Multi-domain]  Cd Length: 533  Bit Score: 76.72  E-value: 1.44e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 397 EVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMktsN--GKYIAPQYIEGK 474
Cdd:COG1021  374 PVPPGEVGELLTRGPYTIRGYYRAPEHNARAFTPDGFYRTGDLVRRTPDGYLVVEGRAKDQI---NrgGEKIAAEEVENL 450
                         90
                 ....*....|....
gi 544685990 475 IGKDKFIEQIAVIA 488
Cdd:COG1021  451 LLAHPAVHDAAVVA 464
A_NRPS_Cytc1-like cd17643
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ...
182-488 1.46e-14

similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341298 [Multi-domain]  Cd Length: 450  Bit Score: 76.19  E-value: 1.46e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 182 IYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDV-----SLSF--------LPLSHIFERAWIAYVFHRGATNCYL 248
Cdd:cd17643   99 IYTSGSTGRPKGVVVSHANVLALFAATQRWFGFNEDDVwtlfhSYAFdfsvweiwGALLHGGRLVVVPYEVARSPEDFAR 178
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 249 EdtkhVRDAlttlKPTVMCAVPrfyekiyTAVwdkvekaplhrRALFNWAIRVGEQHyqieqpsqwLRLQYaladkLVLt 328
Cdd:cd17643  179 L----LRDE----GVTVLNQTP-------SAF-----------YQLVEAADRDGRDP---------LALRY-----VIF- 217
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 klrallggrikmmpcGGAKLE-ASIGSFFHSIG------INiklGYGMTETTATVScW-----QD-KGFNPNSIGTLMPN 395
Cdd:cd17643  218 ---------------GGEALEaAMLRPWAGRFGldrpqlVN---MYGITETTVHVT-FrpldaADlPAAAASPIGRPLPG 278
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 396 ----------AEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGDVGEMDSCGNLFITDRLKELM 458
Cdd:cd17643  279 lrvyvldadgRPVPPGVVGELYVSGAGVARGYLGRPELTAERFVANPFggpgsrmYRTGDLARRLPDGELEYLGRADEQV 358
                        330       340       350
                 ....*....|....*....|....*....|
gi 544685990 459 KTsNGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:cd17643  359 KI-RGFRIELGEIEAALATHPSVRDAAVIV 387
BCL_like cd05919
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ...
34-488 3.90e-14

Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.


Pssm-ID: 341243 [Multi-domain]  Cd Length: 436  Bit Score: 74.81  E-value: 3.90e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  34 QEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFV 113
Cdd:cd05919    9 RSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCEARLVVT 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 114 GDQEqfdqvyqianncpqlmkivamkanmhlrdlpnACYWedflnvvpneaefekrlnskqlsdlftlIYTSGTTGEPKG 193
Cdd:cd05919   89 SADD--------------------------------IAYL----------------------------LYSSGTTGPPKG 108
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 194 VMLDYAN--LAHQLNAHDLaLNVNEDDVSLSflpLSHIFeRAW-----IAYVFHRGATnCYLEDTKHVRDA----LTTLK 262
Cdd:cd05919  109 VMHAHRDplLFADAMAREA-LGLTPGDRVFS---SAKMF-FGYglgnsLWFPLAVGAS-AVLNPGWPTAERvlatLARFR 182
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 263 PTVMCAVPRFYekiytavwdkvekaplhRRALfnwAIRVGEQHyqieqpsqwlrlqyALADklvltklrallggrIKMMP 342
Cdd:cd05919  183 PTVLYGVPTFY-----------------ANLL---DSCAGSPD--------------ALRS--------------LRLCV 214
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 343 CGGAKLEASIGSFF-HSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI----------GEENEILVRGG 411
Cdd:cd05919  215 SAGEALPRGLGERWmEHFGGPILDGIGATEVGHIFLSNRPGAWRLGSTGRPVPGYEIRLvdeeghtippGEEGDLLVRGP 294
                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990 412 MVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:cd05919  295 SAAVGYWNNPEKSRATF-NGGWYRTGDKFCRDADGWYTHAGRADDMLKVG-GQWVSPVEVESLIIQHPAVAEAAVVA 369
PRK06164 PRK06164
acyl-CoA synthetase; Validated
178-491 5.42e-14

acyl-CoA synthetase; Validated


Pssm-ID: 235722 [Multi-domain]  Cd Length: 540  Bit Score: 74.78  E-value: 5.42e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 178 LFTliyTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLEdtkhVRDA 257
Cdd:PRK06164 186 LFT---TSGTTSGPKLVLHRQATLLRHARAIARAYGYDPGAVLLAALPFCGVFGFSTLLGALAGGAPLVCEP----VFDA 258
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 258 LTTLKptvmcavprfyekiytavwdkvekaplhrralfnwaiRVGEQhyqieqpsqwlRLQYALADKLVLTKLRALLGGR 337
Cdd:PRK06164 259 ARTAR-------------------------------------ALRRH-----------RVTHTFGNDEMLRRILDTAGER 290
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 338 ikmMPCGGAKLeASIGSF---FHSI-------GINIKLGYGMTETTATVSCWQ---DKGFNPNSIGTLM-PNAEVKI--- 400
Cdd:PRK06164 291 ---ADFPSARL-FGFASFapaLGELaalararGVPLTGLYGSSEVQALVALQPatdPVSVRIEGGGRPAsPEARVRArdp 366
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 --------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIE 472
Cdd:PRK06164 367 qdgallpdGESGEIEIRAPSLMRGYLDNPDATARALTDDGYFRTGDLGYTRGDGQFVYQTRMGDSLRLG-GFLVNPAEIE 445
                        330
                 ....*....|....*....
gi 544685990 473 gkigkdKFIEQIAVIADAK 491
Cdd:PRK06164 446 ------HALEALPGVAAAQ 458
A_NRPS_PvdJ-like cd17649
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ...
181-488 6.60e-14

non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341304 [Multi-domain]  Cd Length: 450  Bit Score: 73.94  E-value: 6.60e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLShiFErawiayvfhrGATNCYLedtkhvrdaltt 260
Cdd:cd17649   99 VIYTSGSTGTPKGVAVSHGPLAAHCQATAERYGLTPGDRELQFASFN--FD----------GAHEQLL------------ 154
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 261 lkPTVMCA---VPRFYEkiytaVWDKVEKaplHRRALFNWAIRVGEQhyqieQPSQWlrlqYALADKLVLTKLRAllGGR 337
Cdd:cd17649  155 --PPLICGacvVLRPDE-----LWASADE---LAEMVRELGVTVLDL-----PPAYL----QQLAEEADRTGDGR--PPS 213
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 338 IKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQ---DKGFNPNS--IGTLMPN----------AEVKIGE 402
Cdd:cd17649  214 LRLYIFGGEALSPELLRRWLKAPVRLFNAYGPTEATVTPLVWKceaGAARAGASmpIGRPLGGrsayildadlNPVPVGV 293
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 403 ENEILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGKI 475
Cdd:cd17649  294 TGELYIGGEGLARGYLGRPELTAERFVPDPFgapgsrlYRTGDLARWRDDGVIEYLGRVDHQVKI-RGFRIELGEIEAAL 372
                        330
                 ....*....|...
gi 544685990 476 GKDKFIEQIAVIA 488
Cdd:cd17649  373 LEHPGVREAAVVA 385
PRK12467 PRK12467
peptide synthase; Provisional
12-438 3.12e-13

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 73.27  E-value: 3.12e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   12 FRLQAKKWLNRTALRFREQAqwqeMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:PRK12467  518 IEAQARQHPERPALVFGEQV----LSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLD 593
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   92 ATNTAKQVEYIVNDADIKILfVGDQEQFDQVYqianncpqlmkiVAMKANMHLRDLPnacywEDFLNVVPnEAEFEKRLN 171
Cdd:PRK12467  594 PEYPQDRLAYMLDDSGVRLL-LTQSHLLAQLP------------VPAGLRSLCLDEP-----ADLLCGYS-GHNPEVALD 654
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  172 SKQLSdlfTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATnCYLEDT 251
Cdd:PRK12467  655 PDNLA---YVIYTSGSTGQPKGVAISHGALANYVCVIAERLQLAADDSMLMVSTFAFDLGVTELFGALASGAT-LHLLPP 730
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  252 KHVRDAlttlkptvmcavPRFYEKIYTAVWDKVEKAPLHRRALFnwairvgeqhyQIEQPSQWLRLQYALadklvltklr 331
Cdd:PRK12467  731 DCARDA------------EAFAALMADQGVTVLKIVPSHLQALL-----------QASRVALPRPQRALV---------- 777
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  332 allggrikmmpCGGAKLEASIGSFFHSIGINIKL--GYGMTETTATVSCW----QDKGFNPNSIGTLMPNAEVKI----- 400
Cdd:PRK12467  778 -----------CGGEALQVDLLARVRALGPGARLinHYGPTETTVGVSTYelsdEERDFGNVPIGQPLANLGLYIldhyl 846
                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|
gi 544685990  401 -----GEENEILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGD 438
Cdd:PRK12467  847 npvpvGVVGELYIGGAGLARGYHRRPALTAERFVPDPFgadggrlYRTGD 896
A_NRPS_PpsD_like cd17650
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ...
174-500 9.90e-13

similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341305 [Multi-domain]  Cd Length: 447  Bit Score: 70.57  E-value: 9.90e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 174 QLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDV------SLSFLPLSHIFERAWIA----YVFHRGA 243
Cdd:cd17650   91 QPEDLAYVIYTSGTTGKPKGVMVEHRNVAHAAHAWRREYELDSFPVrllqmaSFSFDVFAGDFARSLLNggtlVICPDEV 170
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 244 tncyLEDTKHVRDALTTLKPTVMCAVPrfyekiytavwdkvekaplhrralfnwAIRVGEQHYQIEQpsqwlrlqyalad 323
Cdd:cd17650  171 ----KLDPAALYDLILKSRITLMESTP---------------------------ALIRPVMAYVYRN------------- 206
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 324 KLVLTKLRALLGGRIKMMPCGGAKLEASIGSffHSIGINiklGYGMTETTATVSCWQ-------DKGFNPnsIGTLMPNA 396
Cdd:cd17650  207 GLDLSAMRLLIVGSDGCKAQDFKTLAARFGQ--GMRIIN---SYGVTEATIDSTYYEegrdplgDSANVP--IGRPLPNT 279
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 397 E----------VKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGF------LRTGDVGEMDSCGNLFITDRLKELMKT 460
Cdd:cd17650  280 AmyvlderlqpQPVGVAGELYIGGAGVARGYLNRPELTAERFVENPFapgermYRTGDLARWRADGNVELLGRVDHQVKI 359
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....
gi 544685990 461 sNGKYIAPQYIEGKIGKDKFIEQIAVIA--DAK--KYVSALIVP 500
Cdd:cd17650  360 -RGFRIELGEIESQLARHPAIDEAVVAVreDKGgeARLCAYVVA 402
PRK05857 PRK05857
fatty acid--CoA ligase;
1-493 2.73e-12

fatty acid--CoA ligase;


Pssm-ID: 180293 [Multi-domain]  Cd Length: 540  Bit Score: 69.27  E-value: 2.73e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   1 MASLDFHFVNRFRLQAKKWLNRTALRFREQAQwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGA 80
Cdd:PRK05857   9 MPQLPSTVLDRVFEQARQQPEAIALRRCDGTS--ALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLAC 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  81 MQARAVAVPIYATNTAKQVEYIVNDADIKILFVGDQEQFDqvyqiANNCPQ-LMKIVAMKANMHLRDLPNACYWE-DFLN 158
Cdd:PRK05857  87 AKLGAIAVMADGNLPIAAIERFCQITDPAAALVAPGSKMA-----SSAVPEaLHSIPVIAVDIAAVTRESEHSLDaASLA 161
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 159 VVPNEAEfekrlnskqlSDLFTLIYTSGTTGEPKGVML---DYANLAHQLNAHDLA-LNVNEDDVSLSFLPLSHIFERAW 234
Cdd:PRK05857 162 GNADQGS----------EDPLAMIFTSGTTGEPKAVLLanrTFFAVPDILQKEGLNwVTWVVGETTYSPLPATHIGGLWW 231
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 235 IAYVFHRGATnCYL--EDTKHVRDALTTLKPTVMCAVPRFYEKIYTAVwdkvekaplhrralfnwairvgeqhyqieqps 312
Cdd:PRK05857 232 ILTCLMHGGL-CVTggENTTSLLEILTTNAVATTCLVPTLLSKLVSEL-------------------------------- 278
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 313 qwlrlqyaladKLVLTKLRALlggriKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCW-QDKG----FNPN 387
Cdd:PRK05857 279 -----------KSANATVPSL-----RLVGYGGSRAIAADVRFIEATGVRTAQVYGLSETGCTALCLpTDDGsivkIEAG 342
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 388 SIGTLMPNAEVKIGEEN----------------EILVRGGMVMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNLFIT 451
Cdd:PRK05857 343 AVGRPYPGVDVYLAATDgigptapgagpsasfgTLWIKSPANMLGYWNNPERTAEVLI-DGWVNTGDLLERREDGFFYIK 421
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|..
gi 544685990 452 DRLKElMKTSNGKYIAPQYIegkigkDKFIEQIAVIADAKKY 493
Cdd:PRK05857 422 GRSSE-MIICGGVNIAPDEV------DRIAEGVSGVREAACY 456
A_NRPS_TlmIV_like cd12114
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ...
21-498 4.76e-12

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.


Pssm-ID: 341279 [Multi-domain]  Cd Length: 477  Bit Score: 68.45  E-value: 4.76e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  21 NRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:cd12114    2 DATAVICGDG----TLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAARRE 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKiLFVGDQEQFDQVyqIANNCPQLMKIVAMKAnmhlrdlpnacyWEDFLNVVPneaefekrlnskQLSDLFT 180
Cdd:cd12114   78 AILADAGAR-LVLTDGPDAQLD--VAVFDVLILDLDALAA------------PAPPPPVDV------------APDDLAY 130
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYA---NLAHQLNAHdlaLNVNEDDVSLSFLPLSH------IFE--RAWIAYVFHRGATncyLE 249
Cdd:cd12114  131 VIFTSGSTGTPKGVMISHRaalNTILDINRR---FAVGPDDRVLALSSLSFdlsvydIFGalSAGATLVLPDEAR---RR 204
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 DTKHVRDALTTLKPTVMCAVPrfyekiytavwdkvekaplhrrALFNWAIRVGEQHyQIEQPSqwLRLQYALADKLVLT- 328
Cdd:cd12114  205 DPAHWAELIERHGVTLWNSVP----------------------ALLEMLLDVLEAA-QALLPS--LRLVLLSGDWIPLDl 259
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 --KLRALL-GGRIKMMpcGGAKlEASIGSFFHSIG------INIKLGYGMTETTATVScwqdkgfnpNSIGTLMPnaevk 399
Cdd:cd12114  260 paRLRALApDARLISL--GGAT-EASIWSIYHPIDevppdwRSIPYGRPLANQRYRVL---------DPRGRDCP----- 322
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 400 IGEENEILVRGGMVMRGYYKKPEETAKAFTEDG----FLRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGKI 475
Cdd:cd12114  323 DWVPGELWIGGRGVALGYLGDPELTAARFVTHPdgerLYRTGDLGRYRPDGTLEFLGRRDGQVKV-RGYRIELGEIEAAL 401
                        490       500
                 ....*....|....*....|...
gi 544685990 476 GKDKFIEQIAVIADAKKYVSALI 498
Cdd:cd12114  402 QAHPGVARAVVVVLGDPGGKRLA 424
PtmA cd17636
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ...
181-500 9.46e-12

long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341291 [Multi-domain]  Cd Length: 331  Bit Score: 66.56  E-value: 9.46e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANL-AHQLNAHDLAlNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYledtkhvrdalt 259
Cdd:cd17636    5 AIYTAAFSGRPNGALLSHQALlAQALVLAVLQ-AIDEGTVFLNSGPLFHIGTLMFTLATFHAGGTNVF------------ 71
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 260 tlkptvmcaVPRFyekiytavwDKVEKAPLHRRALFNWAIRVGEQHYQIeqpsqwlrLQYALADKLVLTKLRALLGgrik 339
Cdd:cd17636   72 ---------VRRV---------DAEEVLELIEAERCTHAFLLPPTIDQI--------VELNADGLYDLSSLRSSPA---- 121
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 340 mMPCGGAKLEASIGSFFHSIGiniklGYGMTETTA-TVSCWQDKGFNPNSiGTLMPNAEVKI----------GEENEILV 408
Cdd:cd17636  122 -APEWNDMATVDTSPWGRKPG-----GYGQTEVMGlATFAALGGGAIGGA-GRPSPLVQVRIldedgrevpdGEVGEIVA 194
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 409 RGGMVMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNL-FITDRLKelMKTSNGKYIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:cd17636  195 RGPTVMAGYWNRPEVNARRTR-GGWHHTNDLGRREPDGSLsFVGPKTR--MIKSGAENIYPAEVERCLRQHPAVADAAVI 271
                        330
                 ....*....|....*..
gi 544685990 488 --ADAK--KYVSALIVP 500
Cdd:cd17636  272 gvPDPRwaQSVKAIVVL 288
A_NRPS_ApnA-like cd17644
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ...
170-575 1.05e-11

similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341299 [Multi-domain]  Cd Length: 465  Bit Score: 67.46  E-value: 1.05e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 170 LNSKQLSDLFT-------LIYTSGTTGEPKGVMLDYANLA-HQLNAHDLALNVNEDDVsLSFLPLSHIFERAWIAYVFHR 241
Cdd:cd17644   93 LEDAQISVLLTqpenlayVIYTSGSTGKPKGVMIEHQSLVnLSHGLIKEYGITSSDRV-LQFASIAFDVAAEEIYVTLLS 171
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 242 GATncyledtkhvrdalTTLKPTVMCAVPrfyekiyTAVWDKVEKaplhrralfnWAIRVgeqhYQIeQPSQWLRLQYAL 321
Cdd:cd17644  172 GAT--------------LVLRPEEMRSSL-------EDFVQYIQQ----------WQLTV----LSL-PPAYWHLLVLEL 215
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 322 ADKL--VLTKLRALLGGrikmmpcGGAKLEASIGSFFHSIGINIKL--GYGMTETTATVSCW---QDKGFNPNS--IGTL 392
Cdd:cd17644  216 LLSTidLPSSLRLVIVG-------GEAVQPELVRQWQKNVGNFIQLinVYGPTEATIAATVCrltQLTERNITSvpIGRP 288
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 393 MPNAEVKIGEEN----------EILVRGGMVMRGYYKKPEETAKAFTEDGFL--------RTGDVGEMDSCGNLFITDRL 454
Cdd:cd17644  289 IANTQVYILDENlqpvpvgvpgELHIGGVGLARGYLNRPELTAEKFISHPFNsseserlyKTGDLARYLPDGNIEYLGRI 368
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 455 KELMKTsNGKYIAPQYIEGKIGKDKFIEQIAVIA----DAKKYVSALIVPcfdsleeyakqlnikyqdrielikHSDIIQ 530
Cdd:cd17644  369 DNQVKI-RGFRIELGEIEAVLSQHNDVKTAVVIVredqPGNKRLVAYIVP------------------------HYEESP 423
                        410       420       430       440
                 ....*....|....*....|....*....|....*....|....*..
gi 544685990 531 MFERRIHELQKELPSFeqvkrftLLPQAFSTaMEE--ITPTLKLRRK 575
Cdd:cd17644  424 STVELRQFLKAKLPDY-------MIPSAFVV-LEElpLTPNGKIDRR 462
PRK09029 PRK09029
O-succinylbenzoic acid--CoA ligase; Provisional
17-490 1.42e-11

O-succinylbenzoic acid--CoA ligase; Provisional


Pssm-ID: 236363 [Multi-domain]  Cd Length: 458  Bit Score: 66.82  E-value: 1.42e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  17 KKWLNR----TALRFREQaQWqemSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYA 92
Cdd:PRK09029  10 RHWAQVrpqaIALRLNDE-VL---TWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGARVLPLNP 85
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  93 TNTAKQVEYIVNDADIKILFVGDQEqfdqvyqianncPQLMKIVAmkANMHLRDLPNACYWedflnvvpneaefekrlns 172
Cdd:PRK09029  86 QLPQPLLEELLPSLTLDFALVLEGE------------NTFSALTS--LHLQLVEGAHAVAW------------------- 132
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 173 kQLSDLFTLIYTSGTTGEPKGVmldyanlAHQLNAHdLA-----LNV----NEDDVSLSfLPLSH-----IFERaWIAyv 238
Cdd:PRK09029 133 -QPQRLATMTLTSGSTGLPKAA-------VHTAQAH-LAsaegvLSLmpftAQDSWLLS-LPLFHvsgqgIVWR-WLY-- 199
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 239 fhRGATncyLedtkHVRDAlttlkptvmcavprfyEKIYTAVwDKVEKAPLhrralfnwairVgeqhyqieqPSQWLRLq 318
Cdd:PRK09029 200 --AGAT---L----VVRDK----------------QPLEQAL-AGCTHASL-----------V---------PTQLWRL- 232
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 319 yaLADKLVLTKLRALLggrikmmpCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFnpNSIGTLMPNAEV 398
Cdd:PRK09029 233 --LDNRSEPLSLKAVL--------LGGAAIPVELTEQAEQQGIRCWCGYGLTEMASTVCAKRADGL--AGVGSPLPGREV 300
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 399 KIgEENEILVRGGMVMRGYYKKPEETakAFT-EDGFLRTGDVGEMDScGNLFITDRLKElMKTSNGKYIAPQYIEGKIGK 477
Cdd:PRK09029 301 KL-VDGEIWLRGASLALGYWRQGQLV--PLVnDEGWFATRDRGEWQN-GELTILGRLDN-LFFSGGEGIQPEEIERVINQ 375
                        490
                 ....*....|....*
gi 544685990 478 DKFIEQIAV--IADA 490
Cdd:PRK09029 376 HPLVQQVFVvpVADA 390
PRK07824 PRK07824
o-succinylbenzoate--CoA ligase;
184-487 1.68e-11

o-succinylbenzoate--CoA ligase;


Pssm-ID: 236108 [Multi-domain]  Cd Length: 358  Bit Score: 65.84  E-value: 1.68e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 184 TSGTTGEPKGVMLDYANLAHQLNA-HD---------LALnvneddvslsflPLSHIferAWIAYVfhrgatncyledtkh 253
Cdd:PRK07824  43 TSGTTGTPKGAMLTAAALTASADAtHDrlggpgqwlLAL------------PAHHI---AGLQVL--------------- 92
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 254 VRDALTTLKPTVMcAVPRFYEkiytavwdkVEKAPLHRRALfnwairVGEQHYQIEQPSQWLRLQYALADKLVLTKLRAL 333
Cdd:PRK07824  93 VRSVIAGSEPVEL-DVSAGFD---------PTALPRAVAEL------GGGRRYTSLVPMQLAKALDDPAATAALAELDAV 156
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 334 LggrikmmpCGGAKLEASIGSFFHSIGINIKLGYGMTETTAtvSCWQDkgfnpnsiGTLMPNAEVKIgEENEILVRGGMV 413
Cdd:PRK07824 157 L--------VGGGPAPAPVLDAAAAAGINVVRTYGMSETSG--GCVYD--------GVPLDGVRVRV-EDGRIALGGPTL 217
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 544685990 414 MRGYYKKPEEtaKAFTEDGFLRTGDVGEMDScGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:PRK07824 218 AKGYRNPVDP--DPFAEPGWFRTDDLGALDD-GVLTVLGRADDAISTG-GLTVLPQVVEAALATHPAVADCAVF 287
A_NRPS_AB3403-like cd17646
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ...
12-446 1.71e-11

Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341301 [Multi-domain]  Cd Length: 488  Bit Score: 66.53  E-value: 1.71e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  12 FRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIY 91
Cdd:cd17646    4 VAEQAARTPDAPAVVDEGR----TLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLD 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  92 ATNTAKQVEYIVNDADIKILFVGDQEQfdqvyqianncpqlmkivamkanMHLRDLPNACYWEDFLNVVPNEAEFEKRLN 171
Cdd:cd17646   80 PGYPADRLAYMLADAGPAVVLTTADLA-----------------------ARLPAGGDVALLGDEALAAPPATPPLVPPR 136
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 172 SKQLSdlfTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS---HIFERAWiayVFHRGATncyl 248
Cdd:cd17646  137 PDNLA---YVIYTSGSTGRPKGVMVTHAGIVNRLLWMQDEYPLGPGDRVLQKTPLSfdvSVWELFW---PLVAGAR---- 206
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 249 edtkhvrdalttlkptVMCAVPRfyekiytavwdkVEKAPLHRRALFNwAIRVGEQHYQieqPSQwLRLQYALADKLVLT 328
Cdd:cd17646  207 ----------------LVVARPG------------GHRDPAYLAALIR-EHGVTTCHFV---PSM-LRVFLAEPAAGSCA 253
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 329 KLRALLggrikmmpCGGAKLEASIGSFFHSI-GINIKLGYGMTETTATVSCWQ---DKGFNPNSIGTLMPNAEVKI---- 400
Cdd:cd17646  254 SLRRVF--------CSGEALPPELAARFLALpGAELHNLYGPTEAAIDVTHWPvrgPAETPSVPIGRPVPNTRLYVldda 325
                        410       420       430       440       450       460
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 544685990 401 ------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGF------LRTGDV------GEMDSCG 446
Cdd:cd17646  326 lrpvpvGVPGELYLGGVQLARGYLGRPALTAERFVPDPFgpgsrmYRTGDLarwrpdGALEFLG 389
A_NRPS_SidN3_like cd05918
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ...
174-448 1.73e-11

The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341242 [Multi-domain]  Cd Length: 481  Bit Score: 66.80  E-value: 1.73e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 174 QLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS---HIFErawIAYVFHRGATNCYL-E 249
Cdd:cd05918  104 SPSDAAYVIFTSGSTGKPKGVVIEHRALSTSALAHGRALGLTSESRVLQFASYTfdvSILE---IFTTLAAGGCLCIPsE 180
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 DTKHVR--DALTTLKPTVMCAVPRFyekiytAvwdkvekaplhrRALfnwairvgeqhyqieQPSQWLRLQYaladkLVL 327
Cdd:cd05918  181 EDRLNDlaGFINRLRVTWAFLTPSV------A------------RLL---------------DPEDVPSLRT-----LVL 222
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 328 tklrallggrikmmpcGGAKLEASIGSFFHSigiNIKL--GYGMTETT-ATVSCWQDKGFNPNSIGTLM----------- 393
Cdd:cd05918  223 ----------------GGEALTQSDVDTWAD---RVRLinAYGPAECTiAATVSPVVPSTDPRNIGRPLgatcwvvdpdn 283
                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 394 PNAEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDG-------------FLRTGDVGEMDSCGNL 448
Cdd:cd05918  284 HDRLVPIGAVGELLIEGPILARGYLNDPEKTAAAFIEDPawlkqegsgrgrrLYRTGDLVRYNPDGSL 351
PRK13391 PRK13391
acyl-CoA synthetase; Provisional
61-472 2.14e-11

acyl-CoA synthetase; Provisional


Pssm-ID: 184022 [Multi-domain]  Cd Length: 511  Bit Score: 66.64  E-value: 2.14e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  61 DKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILfVGDQEQFDQVYQIANNCPQL-MKIVAMK 139
Cdd:PRK13391  50 DHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAEAAYIVDDSGARAL-ITSAAKLDVARALLKQCPGVrHRLVLDG 128
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 140 ANmhlrDLPNacyWEDFLNVVpnEAEFEKRLNSKQLSDLftLIYTSGTTGEPKGVM--LDYANLAHQLNAHDLALN---V 214
Cdd:PRK13391 129 DG----ELEG---FVGYAEAV--AGLPATPIADESLGTD--MLYSSGTTGRPKGIKrpLPEQPPDTPLPLTAFLQRlwgF 197
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 215 NEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLEdtkhvrdalttlkptvmcavpRFYEKIYTAVwdkVEKaplhrral 294
Cdd:PRK13391 198 RSDMVYLSPAPLYHSAPQRAVMLVIRLGGTVIVME---------------------HFDAEQYLAL---IEE-------- 245
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 295 fnWAIrvgeQHYQIeQP---SQWLRLQYALADKLVLTKLRALLGGrikMMPCGGAKLEASI---GSFFHSIginiklgYG 368
Cdd:PRK13391 246 --YGV----THTQL-VPtmfSRMLKLPEEVRDKYDLSSLEVAIHA---AAPCPPQVKEQMIdwwGPIIHEY-------YA 308
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 369 MTETTATVSC----WQDkgfNPNSIGTLM---------PNAEVKIGEENEILVRGGMVMRgYYKKPEETAKAFTEDGFLR 435
Cdd:PRK13391 309 ATEGLGFTACdseeWLA---HPGTVGRAMfgdlhilddDGAELPPGEPGTIWFEGGRPFE-YLNDPAKTAEARHPDGTWS 384
                        410       420       430
                 ....*....|....*....|....*....|....*...
gi 544685990 436 T-GDVGEMDSCGNLFITDRlKELMKTSNGKYIAPQYIE 472
Cdd:PRK13391 385 TvGDIGYVDEDGYLYLTDR-AAFMIISGGVNIYPQEAE 421
PRK12316 PRK12316
peptide synthase; Provisional
23-500 3.84e-11

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 66.52  E-value: 3.84e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   23 TALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYI 102
Cdd:PRK12316  528 PALAFGEE----TLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPAERLAYM 603
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  103 VNDADIKILFvgdQEQFDQvyqianncpQLMKIVAMKANMHLrDLPNAcywedFLNVVPNEAEfEKRLNSKQLSdlfTLI 182
Cdd:PRK12316  604 LEDSGVQLLL---SQSHLG---------RKLPLAAGVQVLDL-DRPAA-----WLEGYSEENP-GTELNPENLA---YVI 661
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  183 YTSGTTGEPKGVMLDYANLAHQLNahdlalnvneddvslsflplshiferaWIAYVFHRGATNCYLEDTKHVRDAlttlk 262
Cdd:PRK12316  662 YTSGSTGKPKGAGNRHRALSNRLC---------------------------WMQQAYGLGVGDTVLQKTPFSFDV----- 709
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  263 ptvmcAVPRFYEKIYTAVWDKVEKAPLHRRALFNWAI----RVGEQHYQieqPSQWLRLQYALADKLVLTKLRALLGGRi 338
Cdd:PRK12316  710 -----SVWEFFWPLMSGARLVVAAPGDHRDPAKLVELinreGVDTLHFV---PSMLQAFLQDEDVASCTSLRRIVCSGE- 780
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  339 kmmPCGGAKLEASIGSFFHSIGINIklgYGMTETTATVSCWQ--DKGFNPNSIGTLMPNAE----------VKIGEENEI 406
Cdd:PRK12316  781 ---ALPADAQEQVFAKLPQAGLYNL---YGPTEAAIDVTHWTcvEEGGDSVPIGRPIANLAcyildanlepVPVGVLGEL 854
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  407 LVRGGMVMRGYYKKPEETAKAFTEDGFL------RTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGKIGKDKF 480
Cdd:PRK12316  855 YLAGRGLARGYHGRPGLTAERFVPSPFVagermyRTGDLARYRADGVIEYAGRIDHQVKL-RGLRIELGEIEARLLEHPW 933
                         490       500
                  ....*....|....*....|
gi 544685990  481 IEQIAVIADAKKYVSALIVP 500
Cdd:PRK12316  934 VREAAVLAVDGKQLVGYVVL 953
AFD_YhfT-like cd17633
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ...
177-487 4.02e-11

fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain


Pssm-ID: 341288 [Multi-domain]  Cd Length: 320  Bit Score: 64.35  E-value: 4.02e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 177 DLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLE--DTKHV 254
Cdd:cd17633    1 NPFYIGFTSGTTGLPKAYYRSERSWIESFVCNEDLFNISGEDAILAPGPLSHSLFLYGAISALYLGGTFIGQRkfNPKSW 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 255 RDALTTLKPTVMCAVPRFYEKIYtavwdkvekaplhrralfnwairvgeQHYQIEQPsqwlrlqyaladklvltklrall 334
Cdd:cd17633   81 IRKINQYNATVIYLVPTMLQALA--------------------------RTLEPESK----------------------- 111
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 335 ggrIKMMPCGGAKLEASIGSFFHSI--GINIKLGYGMTETT-ATVSCWQDKGfNPNSIGTLMPNAEVKI-----GEENEI 406
Cdd:cd17633  112 ---IKSIFSSGQKLFESTKKKLKNIfpKANLIEFYGTSELSfITYNFNQESR-PPNSVGRPFPNVEIEIrnadgGEIGKI 187
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 407 LVRGGMVMRGYYKkpeetAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDKFIEQIAV 486
Cdd:cd17633  188 FVKSEMVFSGYVR-----GGFSNPDGWMSVGDIGYVDEEGYLYLVGRESD-MIIIGGINIFPTEIESVLKAIPGIEEAIV 261

                 .
gi 544685990 487 I 487
Cdd:cd17633  262 V 262
PRK12467 PRK12467
peptide synthase; Provisional
11-438 4.91e-11

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 66.34  E-value: 4.91e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   11 RFRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI 90
Cdd:PRK12467 1579 LIEDQAAATPEAVALVFGEQ----ELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPL 1654
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   91 YATNTAKQVEYIVNDADIKILFVgdQEQFDQVYQIANNCPQLmkivamkanmhLRDLPNAcyWEDflnvVPNEAEFEKRL 170
Cdd:PRK12467 1655 DPEYPRERLAYMIEDSGIELLLT--QSHLQARLPLPDGLRSL-----------VLDQEDD--WLE----GYSDSNPAVNL 1715
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  171 NSKQLSdlfTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS---HIFERAW----------IAY 237
Cdd:PRK12467 1716 APQNLA---YVIYTSGSTGRPKGAGNRHGALVNRLCATQEAYQLSAADVVLQFTSFAfdvSVWELFWplingarlviAPP 1792
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  238 VFHRGAtncylEDTKHVrdaLTTLKPTVMCAVPRFYEKIytavwdkVEKAPlhrralfnwairvgeqhyQIEQPsqwlrl 317
Cdd:PRK12467 1793 GAHRDP-----EQLIQL---IERQQVTTLHFVPSMLQQL-------LQMDE------------------QVEHP------ 1833
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  318 qyaladklvlTKLRALLggrikmmpCGGAKLEA-SIGSFFHSIGIN-IKLGYGMTETTATVSCW-----QDKGFNPNSIG 390
Cdd:PRK12467 1834 ----------LSLRRVV--------CGGEALEVeALRPWLERLPDTgLFNLYGPTETAVDVTHWtcrrkDLEGRDSVPIG 1895
                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990  391 TLMPN----------AEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGD 438
Cdd:PRK12467 1896 QPIANlstyildaslNPVPIGVAGELYLGGVGLARGYLNRPALTAERFVADPFgtvgsrlYRTGD 1960
AACS_like cd05968
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ...
18-193 5.98e-11

Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.


Pssm-ID: 341272 [Multi-domain]  Cd Length: 610  Bit Score: 65.20  E-value: 5.98e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  18 KWL----NRTALRFR-EQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYA 92
Cdd:cd05968   69 KWLadtrTRPALRWEgEDGTSRTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFS 148
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  93 TNTAKQVEYIVNDADIKILFVGDQ--------EQFDQVYQIANNCPQLMKIVAMKanmHL-RDLPNACY----WEDFLNV 159
Cdd:cd05968  149 GFGKEAAATRLQDAEAKALITADGftrrgrevNLKEEADKACAQCPTVEKVVVVR---HLgNDFTPAKGrdlsYDEEKET 225
                        170       180       190
                 ....*....|....*....|....*....|....
gi 544685990 160 VPNEAEfekRLNSKqlsDLFTLIYTSGTTGEPKG 193
Cdd:cd05968  226 AGDGAE---RTESE---DPLMIIYTSGTTGKPKG 253
PRK05620 PRK05620
long-chain fatty-acid--CoA ligase;
57-487 6.04e-11

long-chain fatty-acid--CoA ligase;


Pssm-ID: 180167 [Multi-domain]  Cd Length: 576  Bit Score: 65.19  E-value: 6.04e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  57 IDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILfVGDQEQFDQVYQIANNCPQLMKIV 136
Cdd:PRK05620  61 ITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMNDQIVHIINHAEDEVI-VADPRLAEQLGEILKECPCVRAVV 139
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 137 ------AMKANMHLRDLPNACYWEDFLN---VVPNEAEFEKRlnskqlsDLFTLIYTSGTTGEPKGVMLDYANL---AHQ 204
Cdd:PRK05620 140 figpsdADSAAAHMPEGIKVYSYEALLDgrsTVYDWPELDET-------TAAAICYSTGTTGAPKGVVYSHRSLylqSLS 212
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 205 LNAHDlALNVNEDDVSLSFLPLSHIFE-----RAWIA---YVFhRGATncylEDTKHVRDALTTLKPTVMCAVPrfyeki 276
Cdd:PRK05620 213 LRTTD-SLAVTHGESFLCCVPIYHVLSwgvplAAFMSgtpLVF-PGPD----LSAPTLAKIIATAMPRVAHGVP------ 280
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 277 ytAVWDKvekaplhrraLFnwairvgeQHYQIEQPSQwlrlqyaladklvlTKLRALLGGrikmmpcGGAKLEASIGSFF 356
Cdd:PRK05620 281 --TLWIQ----------LM--------VHYLKNPPER--------------MSLQEIYVG-------GSAVPPILIKAWE 319
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 357 HSIGINIKLGYGMTETTA--TVS------CWQDKGFNPNSIGTLMPNAEVKI---GE--------ENEILVRGGMVMRGY 417
Cdd:PRK05620 320 ERYGVDVVHVWGMTETSPvgTVArppsgvSGEARWAYRVSQGRFPASLEYRIvndGQvmestdrnEGEIQVRGNWVTASY 399
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 418 YKKP----------------EETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNGKYIAPQYIEGKIGKDKFI 481
Cdd:PRK05620 400 YHSPteegggaastfrgedvEDANDRFTADGWLRTGDVGSVTRDGFLTIHDRARDVIR-SGGEWIYSAQLENYIMAAPEV 478

                 ....*.
gi 544685990 482 EQIAVI 487
Cdd:PRK05620 479 VECAVI 484
CBAL cd05923
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ...
182-500 7.18e-11

4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.


Pssm-ID: 341247 [Multi-domain]  Cd Length: 493  Bit Score: 64.84  E-value: 7.18e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 182 IYTSGTTGEPKGVMLDYANLAHQ--LNAHDLALNVNEDDVSLSFLPLSH-IFERAWIAYVFHRGATNCYLEDTKHVrDAL 258
Cdd:cd05923  156 FYTSGTTGLPKGAVIPQRAAESRvlFMSTQAGLRHGRHNVVLGLMPLYHvIGFFAVLVAALALDGTYVVVEEFDPA-DAL 234
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 259 TTLKP---TVMCAVPRFYEKIYTAVwdkvEKAPLHRRALfnwairvgeqhyqieqpsQWLRLQYALADKLVLTKLRALLG 335
Cdd:cd05923  235 KLIEQervTSLFATPTHLDALAAAA----EFAGLKLSSL------------------RHVTFAGATMPDAVLERVNQHLP 292
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 336 GRIkmmpcggakleasigsffhsigINIklgYGMTETTATVscwqdkgFNPN-SIGTLMP---NAEVKI----------- 400
Cdd:cd05923  293 GEK----------------------VNI---YGTTEAMNSL-------YMRDaRTGTEMRpgfFSEVRIvriggspdeal 340
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 --GEENEILVR--GGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIG 476
Cdd:cd05923  341 anGEEGELIVAaaADAAFTGYLNQPEATAKKL-QDGWYRTGDVGYVDPSGDVRILGRVDD-MIISGGENIHPSEIERVLS 418
                        330       340
                 ....*....|....*....|....*...
gi 544685990 477 KDKFIEQIAVI--ADAK--KYVSALIVP 500
Cdd:cd05923  419 RHPGVTEVVVIgvADERwgQSVTACVVP 446
A_NRPS_ProA cd17656
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ...
34-500 1.01e-10

gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.


Pssm-ID: 341311 [Multi-domain]  Cd Length: 479  Bit Score: 64.03  E-value: 1.01e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  34 QEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFv 113
Cdd:cd17656   12 QKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIMLDSGVRVVL- 90
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 114 gdqeqfdqvyqianNCPQLMKIVAMKANMHLRDLPNAcYWEDFLNV-VPNEAEfekrlnskqlsDLFTLIYTSGTTGEPK 192
Cdd:cd17656   91 --------------TQRHLKSKLSFNKSTILLEDPSI-SQEDTSNIdYINNSD-----------DLLYIIYTSGTTGKPK 144
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 193 GVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS------HIFErAWIAyvfhrGATnCYL--EDTKHVRDALTTL--- 261
Cdd:cd17656  145 GVQLEHKNMVNLLHFEREKTNINFSDKVLQFATCSfdvcyqEIFS-TLLS-----GGT-LYIirEETKRDVEQLFDLvkr 217
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 262 KPTVMCAVPrfyekiyTAVWdkveKAPLHRRALFN-------WAIRVGEQhYQIEQPSQwlrlqyaladklvltklrall 334
Cdd:cd17656  218 HNIEVVFLP-------VAFL----KFIFSEREFINrfptcvkHIITAGEQ-LVITNEFK--------------------- 264
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 335 ggrikmmpcggakleasigSFFHSIGINIKLGYGMTETTATVSCwqdkGFNPNSIGTLMP-------NAEVKIGEEN--- 404
Cdd:cd17656  265 -------------------EMLHEHNVHLHNHYGPSETHVVTTY----TINPEAEIPELPpigkpisNTWIYILDQEqql 321
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 -------EILVRGGMVMRGYYKKPEETAKAFTEDGF------LRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYI 471
Cdd:cd17656  322 qpqgivgELYISGASVARGYLNRQELTAEKFFPDPFdpnermYRTGDLARYLPDGNIEFLGRADHQVKI-RGYRIELGEI 400
                        490       500       510
                 ....*....|....*....|....*....|...
gi 544685990 472 EGKIGKDKFIEQIAVIADA----KKYVSALIVP 500
Cdd:cd17656  401 EAQLLNHPGVSEAVVLDKAddkgEKYLCAYFVM 433
PRK04813 PRK04813
D-alanine--poly(phosphoribitol) ligase subunit DltA;
367-579 1.49e-10

D-alanine--poly(phosphoribitol) ligase subunit DltA;


Pssm-ID: 235313 [Multi-domain]  Cd Length: 503  Bit Score: 63.76  E-value: 1.49e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 367 YGMTETTATVSCWQ------DKgFNPNSIGTLMPNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAF-T 429
Cdd:PRK04813 293 YGPTEATVAVTSIEitdemlDQ-YKRLPIGYAKPDSPLLIideegtklpdGEQGEIVISGPSVSKGYLNNPEKTAEAFfT 371
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 430 EDG--FLRTGDVGEMDScGNLFITDRLKELMKTsNGKYIAPQYIEGKIGKDKFIEQiAVIADAKK-----YVSALIVPCF 502
Cdd:PRK04813 372 FDGqpAYHTGDAGYLED-GLLFYQGRIDFQIKL-NGYRIELEEIEQNLRQSSYVES-AVVVPYNKdhkvqYLIAYVVPKE 448
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 503 DSLE-EYAKQLNIKyqdrielikhsdiiqmferriHELQKELPSFeqvkrftLLPQAFsTAMEEI--TPTLKLRRKVIMQ 579
Cdd:PRK04813 449 EDFErEFELTKAIK---------------------KELKERLMEY-------MIPRKF-IYRDSLplTPNGKIDRKALIE 499
PLN02479 PLN02479
acetate-CoA ligase
37-490 1.75e-10

acetate-CoA ligase


Pssm-ID: 178097 [Multi-domain]  Cd Length: 567  Bit Score: 63.71  E-value: 1.75e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  37 SWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgDQ 116
Cdd:PLN02479  47 TWAQTYQRCRRLASALAKRSIGPGSTVAVIAPNIPAMYEAHFGVPMAGAVVNCVNIRLNAPTIAFLLEHSKSEVVMV-DQ 125
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 117 EQF---DQVYQI-----ANNCPQLMKIVAMKANMHLRDLPNA-----CYWEDFLNVvpNEAEFekrlNSKQLSDLFTLI- 182
Cdd:PLN02479 126 EFFtlaEEALKIlaekkKSSFKPPLLIVIGDPTCDPKSLQYAlgkgaIEYEKFLET--GDPEF----AWKPPADEWQSIa 199
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 183 --YTSGTTGEPKGVMLdyanlaHQLNAHDLALN------VNEDDVSLSFLPLSHIfeRAWI---AYVFHRGATNCYLE-D 250
Cdd:PLN02479 200 lgYTSGTTASPKGVVL------HHRGAYLMALSnaliwgMNEGAVYLWTLPMFHC--NGWCftwTLAALCGTNICLRQvT 271
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 251 TKHVRDALTTLKPTVMCAVPrfyekiytAVWDKVEKAPLHRRALfnwairvgeqhyqieqPsqwlrlqyaladklvltkl 330
Cdd:PLN02479 272 AKAIYSAIANYGVTHFCAAP--------VVLNTIVNAPKSETIL----------------P------------------- 308
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 331 ralLGGRIKMMPCGGAKlEASIGSFFHSIGINIKLGYGMTET--TATVSCWQDK--GFNPNSIGTLMPNAEVK-IGEEN- 404
Cdd:PLN02479 309 ---LPRVVHVMTAGAAP-PPSVLFAMSEKGFRVTHTYGLSETygPSTVCAWKPEwdSLPPEEQARLNARQGVRyIGLEGl 384
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 -------------------EILVRGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNGKY 465
Cdd:PLN02479 385 dvvdtktmkpvpadgktmgEIVMRGNMVMKGYLKNPKANEEAF-ANGWFHSGDLGVKHPDGYIEIKDRSKDII-ISGGEN 462
                        490       500
                 ....*....|....*....|....*
gi 544685990 466 IAPQYIEGKIGKDKFIEQIAVIADA 490
Cdd:PLN02479 463 ISSLEVENVVYTHPAVLEASVVARP 487
MACS_like_3 cd05971
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
46-512 1.78e-10

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341275 [Multi-domain]  Cd Length: 439  Bit Score: 63.22  E-value: 1.78e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  46 DRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILfvgdqeqfdqvyqi 125
Cdd:cd05971   17 NRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASAL-------------- 82
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 126 anncpqlmkivamkanmhLRDLPNacywedflnvvpneaefekrlnskqlsDLFTLIYTSGTTGEPKGVMLDYANLAHQL 205
Cdd:cd05971   83 ------------------VTDGSD---------------------------DPALIIYTSGTTGPPKGALHAHRVLLGHL 117
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 206 NAHDLALNVNEDDVSLSFLPLshifERAWIAYVF-------HRGAtncyledtkhvrdalttlkPTVMCAVPRFY-EKIY 277
Cdd:cd05971  118 PGVQFPFNLFPRDGDLYWTPA----DWAWIGGLLdvllpslYFGV-------------------PVLAHRMTKFDpKAAL 174
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 278 TAVWD-KVEKAPLHRRALfnwaiRVGEQHYqiEQPSQWLRlqyaladklvltKLRALLggrikmmpCGGAKL-EASIGSF 355
Cdd:cd05971  175 DLMSRyGVTTAFLPPTAL-----KMMRQQG--EQLKHAQV------------KLRAIA--------TGGESLgEELLGWA 227
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 356 FHSIGINIKLGYGMTETTATV-SCWQDKGFNPNSIGTLMPNAEVKI----------GEENEILVR--GGMVMRGYYKKPE 422
Cdd:cd05971  228 REQFGVEVNEFYGQTECNLVIgNCSALFPIKPGSMGKPIPGHRVAIvddngtplppGEVGEIAVElpDPVAFLGYWNNPS 307
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 423 ETAKAFTEDgFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVIA--DAKK--YVSALI 498
Cdd:cd05971  308 ATEKKMAGD-WLLTGDLGRKDSDGYFWYVGRDDDVITSS-GYRIGPAEIEECLLKHPAVLMAAVVGipDPIRgeIVKAFV 385
                        490
                 ....*....|....*.
gi 544685990 499 V--PCFDSLEEYAKQL 512
Cdd:cd05971  386 VlnPGETPSDALAREI 401
PRK12316 PRK12316
peptide synthase; Provisional
7-500 2.10e-10

peptide synthase; Provisional


Pssm-ID: 237054 [Multi-domain]  Cd Length: 5163  Bit Score: 64.21  E-value: 2.10e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990    7 HFVN-RFRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARA 85
Cdd:PRK12316 2003 PGVHqRIAEQAARAPEAIAVVFGDQ----HLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGG 2078
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   86 VAVPIYATNTAKQVEYIVNDADIKILFVgdQEQFDQVYQIANNCPQLmkivamkanmhlrDLPNACYWEDFLNVVPneae 165
Cdd:PRK12316 2079 AYVPLDPNYPAERLAYMLEDSGAALLLT--QRHLLERLPLPAGVARL-------------PLDRDAEWADYPDTAP---- 2139
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  166 fekrLNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLS-HIFERAWI------AYV 238
Cdd:PRK12316 2140 ----AVQLAGENLAYVIYTSGSTGLPKGVAVSHGALVAHCQAAGERYELSPADCELQFMSFSfDGAHEQWFhpllngARV 2215
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  239 FHRGATncyLEDTKHVRDALTTLKPTVMCAVPRFYEKIytavwdkVEKAPLHRRALfnwAIRV----GEQhyqieQPSQW 314
Cdd:PRK12316 2216 LIRDDE---LWDPEQLYDEMERHGVTILDFPPVYLQQL-------AEHAERDGRPP---AVRVycfgGEA-----VPAAS 2277
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  315 LRLQyaladklvltkLRALLGGRIkmmpcggakleasigsffhsigINiklGYGMTETTATVS----CWQDK-GFNPNSI 389
Cdd:PRK12316 2278 LRLA-----------WEALRPVYL----------------------FN---GYGPTEAVVTPLlwkcRPQDPcGAAYVPI 2321
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  390 GTLMPNAEVKIGEENEILVRGGM----------VMRGYYKKPEETAKAFTEDGF-------LRTGDVGEMDSCGNLFITD 452
Cdd:PRK12316 2322 GRALGNRRAYILDADLNLLAPGMagelylggegLARGYLNRPGLTAERFVPDPFsasgerlYRTGDLARYRADGVVEYLG 2401
                         490       500       510       520       530
                  ....*....|....*....|....*....|....*....|....*....|.
gi 544685990  453 RLKELMKTsNGKYIAPQYIEGKIGKDKFIEQIAVIADAK---KYVSALIVP 500
Cdd:PRK12316 2402 RIDHQVKI-RGFRIELGEIEARLQAHPAVREAVVVAQDGasgKQLVAYVVP 2451
PRK09192 PRK09192
fatty acyl-AMP ligase;
396-472 2.31e-10

fatty acyl-AMP ligase;


Pssm-ID: 236403 [Multi-domain]  Cd Length: 579  Bit Score: 63.49  E-value: 2.31e-10
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990 396 AEVKIGEeneILVRGGMVMRGYYKKpEETAKAFTEDGFLRTGDVGEMdSCGNLFITDRLKELMkTSNGKYIAPQYIE 472
Cdd:PRK09192 407 PERVVGH---ICVRGPSLMSGYFRD-EESQDVLAADGWLDTGDLGYL-LDGYLYITGRAKDLI-IINGRNIWPQDIE 477
MACS_euk cd05928
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ...
19-544 2.36e-10

Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.


Pssm-ID: 341251 [Multi-domain]  Cd Length: 530  Bit Score: 63.25  E-value: 2.36e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  19 WLNRTAlrfreqaqwQEMSWqTFQQ--EIDRFSYALIAQHIDIQ--DKIGIFANNMPRWTIADFGAMQARAVAVPIYATN 94
Cdd:cd05928   32 WVNGKG---------DEVKW-SFRElgSLSRKAANVLSGACGLQrgDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQL 101
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  95 TAKQVEYIVNDADIKILFVGDqEQFDQVYQIANNCPQLMkiVAMKANMHLRDlpnacYWEDFLNVVpNEAEFEKRLNSKQ 174
Cdd:cd05928  102 TAKDILYRLQASKAKCIVTSD-ELAPEVDSVASECPSLK--TKLLVSEKSRD-----GWLNFKELL-NEASTEHHCVETG 172
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 175 LSDLFTLIYTSGTTGEPKGVMLDYANLAH----------QLNAHDLALNVNedDVSLSFLPLSHIFErAWI--AYVF-HR 241
Cdd:cd05928  173 SQEPMAIYFTSGTTGSPKMAEHSHSSLGLglkvngrywlDLTASDIMWNTS--DTGWIKSAWSSLFE-PWIqgACVFvHH 249
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 242 GATNcyleDTKHVRDALTTLKPTVMCAVPRFYEKIytaVWDKVEKaplhrralfnwairvgeqhYQIEqpsqwlRLQYAL 321
Cdd:cd05928  250 LPRF----DPLVILKTLSSYPITTFCGAPTVYRML---VQQDLSS-------------------YKFP------SLQHCV 297
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 322 adklvltklrallggrikmmpCGGAKLEASI-GSFFHSIGINIKLGYGMTETtaTVSCWQDKG--FNPNSIGTLMPNAEV 398
Cdd:cd05928  298 ---------------------TGGEPLNPEVlEKWKAQTGLDIYEGYGQTET--GLICANFKGmkIKPGSMGKASPPYDV 354
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 399 KI----------GEENEILVRGGMV-----MRGYYKKPEETAKAFTEDgFLRTGDVGEMDSCGNLFITDRLKELMKTSnG 463
Cdd:cd05928  355 QIiddngnvlppGTEGDIGIRVKPIrpfglFSGYVDNPEKTAATIRGD-FYLTGDRGIMDEDGYFWFMGRADDVINSS-G 432
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 464 KYIAPQYIEGKIGKDKFIEQIAVIADAK----KYVSALIVPCFDSLEEYAKQLNIKYQDRielIKHSDIIQMFERRIhEL 539
Cdd:cd05928  433 YRIGPFEVESALIEHPAVVESAVVSSPDpirgEVVKAFVVLAPQFLSHDPEQLTKELQQH---VKSVTAPYKYPRKV-EF 508

                 ....*
gi 544685990 540 QKELP 544
Cdd:cd05928  509 VQELP 513
PRK08276 PRK08276
long-chain-fatty-acid--CoA ligase; Validated
61-473 4.68e-10

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236215 [Multi-domain]  Cd Length: 502  Bit Score: 62.23  E-value: 4.68e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  61 DKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgDQEQFDQVYQIANNCPQLMKIVAMKA 140
Cdd:PRK08276  37 DVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVLIV-SAALADTAAELAAELPAGVPLLLVVA 115
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 141 NmhlrDLPNACYWEDFLNVVPNEAEFEKRLNSkqlsdlfTLIYTSGTTGEPKGVM-------LDYANLAHqLNAHDLALN 213
Cdd:PRK08276 116 G----PVPGFRSYEEALAAQPDTPIADETAGA-------DMLYSSGTTGRPKGIKrplpgldPDEAPGMM-LALLGFGMY 183
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 214 VNEDDVSLSFLPLSHIFERAWIAYVFHRGATNCYLEdtkhvR-DALTTLKptvmcavprfyekiytavwdkvekaplhrr 292
Cdd:PRK08276 184 GGPDSVYLSPAPLYHTAPLRFGMSALALGGTVVVME-----KfDAEEALA------------------------------ 228
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 293 alfnwAIrvgeQHYQIEQpSQW--------LRLQYALADKLVLTKLR-ALLGGrikmMPCGGAKLEASI---GSFFHSIg 360
Cdd:PRK08276 229 -----LI----ERYRVTH-SQLvptmfvrmLKLPEEVRARYDVSSLRvAIHAA----APCPVEVKRAMIdwwGPIIHEY- 293
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 361 iniklgYGMTE----TTATVSCWQDKgfnPNSIGTLMpNAEVKI----------GEENEILVRGGMVMRGYYKKPEETAK 426
Cdd:PRK08276 294 ------YASSEgggvTVITSEDWLAH---PGSVGKAV-LGEVRIldedgnelppGEIGTVYFEMDGYPFEYHNDPEKTAA 363
                        410       420       430       440
                 ....*....|....*....|....*....|....*....|....*..
gi 544685990 427 AFTEDGFLRTGDVGEMDSCGNLFITDRlKELMKTSNGKYIAPQYIEG 473
Cdd:PRK08276 364 ARNPHGWVTVGDVGYLDEDGYLYLTDR-KSDMIISGGVNIYPQEIEN 409
ABCL cd05958
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ...
22-554 8.53e-10

2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.


Pssm-ID: 341268 [Multi-domain]  Cd Length: 439  Bit Score: 61.34  E-value: 8.53e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  22 RTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQ-DKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:cd05958    1 RTCLRSPER----EWTYRDLLALANRIANVLVGELGIVPgNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELA 76
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILFVGDQEQfdqvyqianncpqlmkivamkanmhLRDlpnacywedflnvvpneaefekrlnskqlsDLFT 180
Cdd:cd05958   77 YILDKARITVALCAHALT-------------------------ASD------------------------------DICI 101
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVM---LDYANLAHQLNAHDLALnvNEDDVSLSFLPLSHIFERAWIA-YVFHRGATNCYLEDT--KHV 254
Cdd:cd05958  102 LAFTSGTTGAPKATMhfhRDPLASADRYAVNVLRL--REDDRFVGSPPLAFTFGLGGVLlFPFGVGASGVLLEEAtpDLL 179
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 255 RDALTTLKPTVMCAVPRFYEKIytavwdkVEKAPLHRRalfnwairvgeqhyqieqpsqwlrlqyaladklvltklraLL 334
Cdd:cd05958  180 LSAIARYKPTVLFTAPTAYRAM-------LAHPDAAGP----------------------------------------DL 212
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 335 GGRIKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI----------GEEN 404
Cdd:cd05958  213 SSLRKCVSAGEALPAALHRAWKEATGIPIIDGIGSTEMFHIFISARPGDARPGATGKPVPGYEAKVvddegnpvpdGTIG 292
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 EILVRGGMvmrGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKtSNGKYIAPQYIEGKIGKDKFIEQI 484
Cdd:cd05958  293 RLAVRGPT---GCRYLADKRQRTYVQGGWNITGDTYSRDPDGYFRHQGRSDDMIV-SGGYNIAPPEVEDVLLQHPAVAEC 368
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 485 AVIA----DAKKYVSALIVP---------CFDSLEEYAKQLNIKYQdrielikhsdiiqmFERRIhELQKELPSFE--QV 549
Cdd:cd05958  369 AVVGhpdeSRGVVVKAFVVLrpgvipgpvLARELQDHAKAHIAPYK--------------YPRAI-EFVTELPRTAtgKL 433

                 ....*
gi 544685990 550 KRFTL 554
Cdd:cd05958  434 QRFAL 438
MACS_like_4 cd05969
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ...
40-487 1.05e-09

Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.


Pssm-ID: 341273 [Multi-domain]  Cd Length: 442  Bit Score: 60.98  E-value: 1.05e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  40 TFQQ---EIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgdq 116
Cdd:cd05969    2 TFAQlkvLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLIT--- 78
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 117 eqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpNEAEFEKRlnskQLSDLFTLIYTSGTTGEPKGVML 196
Cdd:cd05969   79 ---------------------------------------------TEELYERT----DPEDPTLLHYTSGTTGTPKGVLH 109
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 197 DYANLAHQLNAHDLALNVNEDDVslsflpLSHIFERAWIAYVFH-------RGATNCYLEDTKHVR---DALTTLKPTVM 266
Cdd:cd05969  110 VHDAMIFYYFTGKYVLDLHPDDI------YWCTADPGWVTGTVYgiwapwlNGVTNVVYEGRFDAEswyGIIERVKVTVW 183
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 267 cavprfyekiYTAvwdkvekaPLHRRALfnwairvgeqhyqieqpsqwLRLQYALADKLVLTKLRALLGGrikmmpcgGA 346
Cdd:cd05969  184 ----------YTA--------PTAIRML--------------------MKEGDELARKYDLSSLRFIHSV--------GE 217
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 347 KLEASIGSFFHSI-GINIKLGYGMTETTATVSC-WQDKGFNPNSIGTLMPNAEVKI----------GEENEILVRGGM-- 412
Cdd:cd05969  218 PLNPEAIRWGMEVfGVPIHDTWWQTETGSIMIAnYPCMPIKPGSMGKPLPGVKAAVvdengnelppGTKGILALKPGWps 297
                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 413 VMRGYYKKPEETAKAFTeDGFLRTGDVGEMDSCGNLFITDRLKELMKTSnGKYIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:cd05969  298 MFRGIWNDEERYKNSFI-DGWYLTGDLAYRDEDGYFWFVGRADDIIKTS-GHRVGPFEVESALMEHPAVAEAGVI 370
PRK07445 PRK07445
O-succinylbenzoic acid--CoA ligase; Reviewed
360-500 1.63e-09

O-succinylbenzoic acid--CoA ligase; Reviewed


Pssm-ID: 236019 [Multi-domain]  Cd Length: 452  Bit Score: 60.39  E-value: 1.63e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 360 GINIKLGYGMTETTATVSCWQDKGF--NPNSIGTLMPNAEVKI--GEENEILVRGGMVMRGYYKKPEETAKAFTedgflr 435
Cdd:PRK07445 254 QLRLAPTYGMTETASQIATLKPDDFlaGNNSSGQVLPHAQITIpaNQTGNITIQAQSLALGYYPQILDSQGIFE------ 327
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 544685990 436 TGDVGEMDSCGNLFITDRLKELMkTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKY----VSALIVP 500
Cdd:PRK07445 328 TDDLGYLDAQGYLHILGRNSQKI-ITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHwgevVTAIYVP 395
PRK09274 PRK09274
peptide synthase; Provisional
182-465 2.90e-09

peptide synthase; Provisional


Pssm-ID: 236443 [Multi-domain]  Cd Length: 552  Bit Score: 59.91  E-value: 2.90e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 182 IYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAW-IAYV-----FHRGATncylEDTKHVR 255
Cdd:PRK09274 180 LFTSGSTGTPKGVVYTHGMFEAQIEALREDYGIEPGEIDLPTFPLFALFGPALgMTSVipdmdPTRPAT----VDPAKLF 255
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 256 DALTTLKPTVMCAVPrfyekiytAVWDKVekaplhrralfnwairvgeqhyqieqpsqwlrLQYALADKLVLTKLRALLg 335
Cdd:PRK09274 256 AAIERYGVTNLFGSP--------ALLERL--------------------------------GRYGEANGIKLPSLRRVI- 294
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 336 grikmmpCGGAKLEASIGSFFHSI---GINIKLGYGMTET------------TATVSCWqDKGFNpNSIGTLMPNAEVKI 400
Cdd:PRK09274 295 -------SAGAPVPIAVIERFRAMlppDAEILTPYGATEAlpissiesreilFATRAAT-DNGAG-ICVGRPVDGVEVRI 365
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -------------------GEENEILVRGGMVMRGYYKKPEETAKAFTEDG----FLRTGDVGEMDSCGNLFITDRLKEL 457
Cdd:PRK09274 366 iaisdapipewddalrlatGEIGEIVVAGPMVTRSYYNRPEATRLAKIPDGqgdvWHRMGDLGYLDAQGRLWFCGRKAHR 445

                 ....*...
gi 544685990 458 MKTSNGKY 465
Cdd:PRK09274 446 VETAGGTL 453
PRK13382 PRK13382
bile acid CoA ligase;
36-511 3.87e-09

bile acid CoA ligase;


Pssm-ID: 172019 [Multi-domain]  Cd Length: 537  Bit Score: 59.39  E-value: 3.87e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  36 MSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILFVgD 115
Cdd:PRK13382  69 LTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGADILLLNTSFAGPALAEVVTREGVDTVIY-D 147
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 116 QEQFDQVYQIANNCPQLMKIVAmkanmhlrdlpnacyWEDFLNVVPNEAEFEKRLN-----SKQLSDlfTLIYTSGTTGE 190
Cdd:PRK13382 148 EEFSATVDRALADCPQATRIVA---------------WTDEDHDLTVEVLIAAHAGqrpepTGRKGR--VILLTSGTTGT 210
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 191 PKGVmldyanlahqlnahdlalnvnEDDVSLSFLPLSHIFERA-W--------IAYVFHR---------GATNCYL---- 248
Cdd:PRK13382 211 PKGA---------------------RRSGPGGIGTLKAILDRTpWraeeptviVAPMFHAwgfsqlvlaASLACTIvtrr 269
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 249 ----EDTKHVRDALttlKPTVMCAVPRFYEKIYTAVwDKVeKAPLHRRALfnwairvgeqhyqieqpsqwlrlqyaladk 324
Cdd:PRK13382 270 rfdpEATLDLIDRH---RATGLAVVPVMFDRIMDLP-AEV-RNRYSGRSL------------------------------ 314
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 325 lvltKLRALLGGRikMMPcggakleASIGSFFHSIGINIKLGYGMTE----TTATVscwQDKGFNPNSIGTLMPNAEVKI 400
Cdd:PRK13382 315 ----RFAAASGSR--MRP-------DVVIAFMDQFGDVIYNNYNATEagmiATATP---ADLRAAPDTAGRPAEGTEIRI 378
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 ----------GEENEILVRGGMVMRGYYKKpeeTAKAFtEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQY 470
Cdd:PRK13382 379 ldqdfrevptGEVGTIFVRNDTQFDGYTSG---STKDF-HDGFMASGDVGYLDENGRLFVVGRDDE-MIVSGGENVYPIE 453
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....*..
gi 544685990 471 IEGKIGKDKFIEQIAVIA-DAKKY---VSALIVPCFDS--LEEYAKQ 511
Cdd:PRK13382 454 VEKTLATHPDVAEAAVIGvDDEQYgqrLAAFVVLKPGAsaTPETLKQ 500
A_NRPS_Sfm_like cd12115
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ...
6-500 5.06e-09

The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.


Pssm-ID: 341280 [Multi-domain]  Cd Length: 447  Bit Score: 58.87  E-value: 5.06e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   6 FHFVNRfrlQAKKWLNRTALRfreqAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARA 85
Cdd:cd12115    2 HDLVEA---QAARTPDAIALV----CGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGA 74
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  86 VAVPIYATNTAKQVEYIVNDADIKILFVgdqeqfdqvyqianncpqlmkivamkanmhlrdlpnacywedflnvvpneae 165
Cdd:cd12115   75 AYVPLDPAYPPERLRFILEDAQARLVLT---------------------------------------------------- 102
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 166 fekrlnskQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLN-------AHDLA--LNVNEDDVSLS----FLPLSHifer 232
Cdd:cd12115  103 --------DPDDLAYVIYTSGSTGRPKGVAIEHRNAAAFLQwaaaafsAEELAgvLASTSICFDLSvfelFGPLAT---- 170
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 233 awiayvfhrGATNCYLEDTKHVRD----ALTTLKPTVMCAVPRFYEkiytavwdkVEKAPLHRRALfNWAirvGEqhyqi 308
Cdd:cd12115  171 ---------GGKVVLADNVLALPDlpaaAEVTLINTVPSAAAELLR---------HDALPASVRVV-NLA---GE----- 223
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 309 eqpsqwlrlqyALADKLVLtKLRALLGG-RIkmmpcggakleasigsffhsigINIklgYGMTETT--ATVSCWQDKGFN 385
Cdd:cd12115  224 -----------PLPRDLVQ-RLYARLQVeRV----------------------VNL---YGPSEDTtySTVAPVPPGASG 266
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 386 PNSIGTLMPN----------AEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGFL------RTGDVGEMDSCGNLF 449
Cdd:cd12115  267 EVSIGRPLANtqayvldralQPVPLGVPGELYIGGAGVARGYLGRPGLTAERFLPDPFGpgarlyRTGDLVRWRPDGLLE 346
                        490       500       510       520       530
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 450 ITDRLKELMKTsNGKYIAPQYIEGKIGKDKFIEQIAVI----ADAKKYVSALIVP 500
Cdd:cd12115  347 FLGRADNQVKV-RGFRIELGEIEAALRSIPGVREAVVVaigdAAGERRLVAYIVA 400
PRK06814 PRK06814
acyl-[ACP]--phospholipid O-acyltransferase;
181-459 2.91e-08

acyl-[ACP]--phospholipid O-acyltransferase;


Pssm-ID: 235865 [Multi-domain]  Cd Length: 1140  Bit Score: 56.90  E-value: 2.91e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  181 LIYTSGTTGEPKGVMLDYANLahQLNAHDLALNV--NEDDVSLSFLPLSHIFerawiayvfhrGATNCYLedtkhvrdaL 258
Cdd:PRK06814  798 ILFTSGSEGTPKGVVLSHRNL--LANRAQVAARIdfSPEDKVFNALPVFHSF-----------GLTGGLV---------L 855
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  259 TTLK--PTVMCAVPRFYEKIYTAVWDKvekaplhrralfNWAIRVGEQHY-----QIEQPSQWLRLQYALAdklvltklr 331
Cdd:PRK06814  856 PLLSgvKVFLYPSPLHYRIIPELIYDT------------NATILFGTDTFlngyaRYAHPYDFRSLRYVFA--------- 914
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  332 allggrikmmpcGGAKLEASIGSFF-HSIGINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVK------IGEEN 404
Cdd:PRK06814  915 ------------GAEKVKEETRQTWmEKFGIRILEGYGVTETAPVIALNTPMHNKAGTVGRLLPGIEYRlepvpgIDEGG 982
                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  405 EILVRGGMVMRGYYK--KP---EETakaftEDGFLRTGDVGEMDSCGNLFITDRLKELMK 459
Cdd:PRK06814  983 RLFVRGPNVMLGYLRaeNPgvlEPP-----ADGWYDTGDIVTIDEEGFITIKGRAKRFAK 1037
A_NRPS_CmdD_like cd17652
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ...
174-488 4.00e-08

similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).


Pssm-ID: 341307 [Multi-domain]  Cd Length: 436  Bit Score: 55.72  E-value: 4.00e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 174 QLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLShiFERA-W-IAYVFHRGATnCYLEDT 251
Cdd:cd17652   91 TPDNLAYVIYTSGSTGRPKGVVVTHRGLANLAAAQIAAFDVGPGSRVLQFASPS--FDASvWeLLMALLAGAT-LVLAPA 167
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 252 ------KHVRDALT-------TLKPTVMCAVPrfyekiytavwdkvEKAPLHRRALfnwaIRVGEqhyqieqpsqwlrlq 318
Cdd:cd17652  168 eellpgEPLADLLRehrithvTLPPAALAALP--------------PDDLPDLRTL----VVAGE--------------- 214
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 319 yALADKLVltklRALLGGRiKMmpcggakleasigsffhsigINiklGYGMTETT--ATVS-CwqDKGFNPNSIGTLMPN 395
Cdd:cd17652  215 -ACPAELV----DRWAPGR-RM--------------------IN---AYGPTETTvcATMAgP--LPGGGVPPIGRPVPG 263
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 396 AEVKI----------GEENEILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGDVGEMDSCGNLFITDRLKELM 458
Cdd:cd17652  264 TRVYVldarlrpvppGVPGELYIAGAGLARGYLNRPGLTAERFVADPFgapgsrmYRTGDLARWRADGQLEFLGRADDQV 343
                        330       340       350
                 ....*....|....*....|....*....|
gi 544685990 459 KTsNGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:cd17652  344 KI-RGFRIELGEVEAALTEHPGVAEAVVVV 372
MACS_like_1 cd05974
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ...
360-487 4.07e-08

Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.


Pssm-ID: 341278 [Multi-domain]  Cd Length: 432  Bit Score: 55.65  E-value: 4.07e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 360 GINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVKI-------GEENEILV-----RGGMVMRGYYKKPEETAKA 427
Cdd:cd05974  225 GLTIRDGYGQTETTALVGNSPGQPVKAGSMGRPLPGYRVALldpdgapATEGEVALdlgdtRPVGLMKGYAGDPDKTAHA 304
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 428 FtEDGFLRTGDVGEMDSCGNLFITDRLKELMKTSNGKyIAPQYIEGKIGKDKFIEQIAVI 487
Cdd:cd05974  305 M-RGGYYRTGDIAMRDEDGYLTYVGRADDVFKSSDYR-ISPFELESVLIEHPAVAEAAVV 362
PRK07768 PRK07768
long-chain-fatty-acid--CoA ligase; Validated
184-472 9.17e-08

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 236091 [Multi-domain]  Cd Length: 545  Bit Score: 55.00  E-value: 9.17e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 184 TSGTTGEPKGVMLDYANLAHQLNAHDLALNVN-EDDVSLSFLPLSHifERAWIAYV---FHRGATNCYLEDTKHVRDAL- 258
Cdd:PRK07768 160 TSGSTGSPKAVQITHGNLYANAEAMFVAAEFDvETDVMVSWLPLFH--DMGMVGFLtvpMYFGAELVKVTPMDFLRDPLl 237
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 259 -----TTLKPTVMCAvPRF-YekiytavwdkvekAPLHRRalfnwaIRVGEQHYQIEQPSqwlrLQYAL--ADKLVLTKL 330
Cdd:PRK07768 238 waeliSKYRGTMTAA-PNFaY-------------ALLARR------LRRQAKPGAFDLSS----LRFALngAEPIDPADV 293
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 331 RALL--GGRIKMMPcgGAKLEAsigsffhsiginiklgYGMTETTATVScwqdkgFNP---------------------- 386
Cdd:PRK07768 294 EDLLdaGARFGLRP--EAILPA----------------YGMAEATLAVS------FSPcgaglvvdevdadllaalrrav 349
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 387 ----------NSIGTLMPNAEVKIGEEN----------EILVRGGMVMRGyYKKPEETAKAFTEDGFLRTGDVGEMDSCG 446
Cdd:PRK07768 350 patkgntrrlATLGPPLPGLEVRVVDEDgqvlpprgvgVIELRGESVTPG-YLTMDGFIPAQDADGWLDTGDLGYLTEEG 428
                        330       340
                 ....*....|....*....|....*.
gi 544685990 447 NLFITDRLKELMKTSnGKYIAPQYIE 472
Cdd:PRK07768 429 EVVVCGRVKDVIIMA-GRNIYPTDIE 453
PRK05850 PRK05850
acyl-CoA synthetase; Validated
391-458 1.01e-07

acyl-CoA synthetase; Validated


Pssm-ID: 235624 [Multi-domain]  Cd Length: 578  Bit Score: 54.95  E-value: 1.01e-07
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 544685990 391 TLMPNAEVKIGEeneILVRGGMVMRGYYKKPEETAKAF----------TEDG-FLRTGDVGEMdSCGNLFITDRLKELM 458
Cdd:PRK05850 388 TCIECPAGTVGE---IWVHGDNVAAGYWQKPEETERTFgatlvdpspgTPEGpWLRTGDLGFI-SEGELFIVGRIKDLL 462
PRK04319 PRK04319
acetyl-CoA synthetase; Provisional
21-219 1.11e-07

acetyl-CoA synthetase; Provisional


Pssm-ID: 235279 [Multi-domain]  Cd Length: 570  Bit Score: 54.90  E-value: 1.11e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  21 NRTALRFREQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:PRK04319  59 DKVALRYLDASRKEKYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVR 138
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILfVGDQEQFDQVyqIANNCPQLMKIVAMKAnmHLRDLPNACYWEDFLNVVPNEAEFEkrlnSKQLSDLFT 180
Cdd:PRK04319 139 DRLEDSEAKVL-ITTPALLERK--PADDLPSLKHVLLVGE--DVEEGPGTLDFNALMEQASDEFDIE----WTDREDGAI 209
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVM-LDYANLAHQLNAHdLALNVNEDDV 219
Cdd:PRK04319 210 LHYTSGSTGKPKGVLhVHNAMLQHYQTGK-YVLDLHEDDV 248
entE PRK10946
(2,3-dihydroxybenzoyl)adenylate synthase;
394-472 1.70e-07

(2,3-dihydroxybenzoyl)adenylate synthase;


Pssm-ID: 236803 [Multi-domain]  Cd Length: 536  Bit Score: 54.23  E-value: 1.70e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 394 PNAEVKIGEEN-EILVRG--GMVM-------RGYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMkTSNG 463
Cdd:PRK10946 361 PDDEVWVADADgNPLPQGevGRLMtrgpytfRGYYKSPQHNASAFDANGFYCSGDLVSIDPDGYITVVGREKDQI-NRGG 439

                 ....*....
gi 544685990 464 KYIAPQYIE 472
Cdd:PRK10946 440 EKIAAEEIE 448
A_NRPS_ACVS-like cd17648
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ...
176-488 4.16e-07

N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.


Pssm-ID: 341303 [Multi-domain]  Cd Length: 453  Bit Score: 52.79  E-value: 4.16e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 176 SDLFTLIYTSGTTGEPKGVMLDYANLAHQLNahDLA----LNVNEDDVSLSFlplshiferawIAYVFhrgatNCYLEDT 251
Cdd:cd17648   94 TDLAYAIYTSGTTGKPKGVLVEHGSVVNLRT--SLSeryfGRDNGDEAVLFF-----------SNYVF-----DFFVEQM 155
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 252 KhvrDALTT------LKPTVMCAVPRFYEKIYTavwDKVEkaplhrralfnwairvgeqhYQIEQPSQWLRLQYALADKL 325
Cdd:cd17648  156 T---LALLNgqklvvPPDEMRFDPDRFYAYINR---EKVT--------------------YLSGTPSVLQQYDLARLPHL 209
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 326 vltklrallggriKMMPCGGAKLEAS----IGSFFHSIGINiklGYGMTETTATVSCWQDKGFNP--NSIGTLMPNAE-- 397
Cdd:cd17648  210 -------------KRVDAAGEEFTAPvfekLRSRFAGLIIN---AYGPTETTVTNHKRFFPGDQRfdKSLGRPVRNTKcy 273
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 398 --------VKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGF--------------LRTGDVGEMDSCGNLFITDRlK 455
Cdd:cd17648  274 vlndamkrVPVGAVGELYLGGDGVARGYLNRPELTAERFLPNPFqteqerargrnarlYKTGDLVRWLPSGELEYLGR-N 352
                        330       340       350
                 ....*....|....*....|....*....|...
gi 544685990 456 ELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:cd17648  353 DFQVKIRGQRIEPGEVEAALASYPGVRECAVVA 385
PRK12467 PRK12467
peptide synthase; Provisional
34-575 6.57e-07

peptide synthase; Provisional


Pssm-ID: 237108 [Multi-domain]  Cd Length: 3956  Bit Score: 52.86  E-value: 6.57e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   34 QEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVEYIVNDADIKILfV 113
Cdd:PRK12467 3119 QQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYVPLDPEYPRERLAYMIEDSGVKLL-L 3197
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  114 GDQEQFDQVYQIANNCPQLMKIVAMKAnmhlrdLPNACywedflnvvPNeaefekrlNSKQLSDLFTLIYTSGTTGEPKG 193
Cdd:PRK12467 3198 TQAHLLEQLPAPAGDTALTLDRLDLNG------YSENN---------PS--------TRVMGENLAYVIYTSGSTGKPKG 3254
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  194 VMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLShiFERAwiayvfhrgatncyledtkhVRDALTTLkptvMCAvprfy 273
Cdd:PRK12467 3255 VGVRHGALANHLCWIAEAYELDANDRVLLFMSFS--FDGA--------------------QERFLWTL----ICG----- 3303
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  274 ekiytavwdkvekAPLHRRALFNWAirvGEQHYQieqpsQWLRLQYALADkLVLTKLRALL-------GGRIKMMPCGGA 346
Cdd:PRK12467 3304 -------------GCLVVRDNDLWD---PEELWQ-----AIHAHRISIAC-FPPAYLQQFAedaggadCASLDIYVFGGE 3361
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  347 KL-EASIGSFF-HSIGINIKLGYGMTETTATVSCW---QDKGFNPNS--IGTLMPNAE----------VKIGEENEILVR 409
Cdd:PRK12467 3362 AVpPAAFEQVKrKLKPRGLTNGYGPTEAVVTVTLWkcgGDAVCEAPYapIGRPVAGRSiyvldgqlnpVPVGVAGELYIG 3441
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  410 GGMVMRGYYKKPEETAKAFTEDGFL-------RTGDVGEMDSCGNLFITDRLKELMKTsNGKYIAPQYIEGKIGKDKFIE 482
Cdd:PRK12467 3442 GVGLARGYHQRPSLTAERFVADPFSgsggrlyRTGDLARYRADGVIEYLGRIDHQVKI-RGFRIELGEIEARLLQHPSVR 3520
                         490       500       510       520       530       540       550       560
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  483 QIAVIA---DAKKYVSALIVPcfdsleeyakqlNIKYQD-RIELIKHsdiiqmferriheLQKELPSFEQVKRFTLLPQA 558
Cdd:PRK12467 3521 EAVVLArdgAGGKQLVAYVVP------------ADPQGDwRETLRDH-------------LAASLPDYMVPAQLLVLAAM 3575
                         570
                  ....*....|....*..
gi 544685990  559 fstameEITPTLKLRRK 575
Cdd:PRK12467 3576 ------PLGPNGKVDRK 3586
PRK08043 PRK08043
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
61-500 1.36e-06

bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;


Pssm-ID: 181207 [Multi-domain]  Cd Length: 718  Bit Score: 51.25  E-value: 1.36e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  61 DKIGIFannMPRWTI---ADFGA-MQARAVAVPIYaTNTAKQVEYIVNDADIKILFVgdQEQFDQVYQIANnCPQLMKiv 136
Cdd:PRK08043 256 ERIGLM---LPNATIsaaVIFGAsLRRRIPAMMNY-TAGVKGLTSAITAAEIKTIFT--SRQFLDKGKLWH-LPEQLT-- 326
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 137 amKAN-MHLRDLPNACYWEDFLNV-----VPNEAEFekrlnSKQLSDLFTLIYTSGTTGEPKGVMLDYANLahqlnahdL 210
Cdd:PRK08043 327 --QVRwVYLEDLKDDVTTADKLWIfahllMPRLAQV-----KQQPEDAALILFTSGSEGHPKGVVHSHKSL--------L 391
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 211 AlNVNE---------DDVSLSFLPLSHIFerawiayvfhrGATNCYLedTKHVRDALTTLKPTvmcavPRFYEKIYTAVW 281
Cdd:PRK08043 392 A-NVEQiktiadftpNDRFMSALPLFHSF-----------GLTVGLF--TPLLTGAEVFLYPS-----PLHYRIVPELVY 452
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 282 DKvekaplHRRALFNWAIRVGeqHY-QIEQPSQWLRLQYALAdklvltklrallggrikmmpcGGAKLEASIGS-FFHSI 359
Cdd:PRK08043 453 DR------NCTVLFGTSTFLG--NYaRFANPYDFARLRYVVA---------------------GAEKLQESTKQlWQDKF 503
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 360 GINIKLGYGMTETTATVSCWQDKGFNPNSIGTLMPNAEVK------IGEENEILVRGGMVMRGYYK--KP-------EET 424
Cdd:PRK08043 504 GLRILEGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARllsvpgIEQGGRLQLKGPNIMNGYLRveKPgvlevptAEN 583
                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 425 AKAFTEDGFLRTGDVGEMDSCGNLFITDRLKELMKtsngkyiapqyIEGKIGKDKFIEQIAVIADAKKYVSALIVP 500
Cdd:PRK08043 584 ARGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAK-----------IAGEMVSLEMVEQLALGVSPDKQHATAIKS 648
BACL_like cd05929
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ...
397-472 1.82e-06

Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.


Pssm-ID: 341252 [Multi-domain]  Cd Length: 473  Bit Score: 50.84  E-value: 1.82e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 397 EVKIGEENEILVRGGMVMRgYYKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRlKELMKTSNGKYIAPQYIE 472
Cdd:cd05929  316 EVPPGEIGEVYFANGPGFE-YTNDPEKTAAARNEGGWSTLGDVGYLDEDGYLYLTDR-RSDMIISGGVNIYPQEIE 389
PRK08279 PRK08279
long-chain-acyl-CoA synthetase; Validated
11-228 1.89e-06

long-chain-acyl-CoA synthetase; Validated


Pssm-ID: 236217 [Multi-domain]  Cd Length: 600  Bit Score: 50.64  E-value: 1.89e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  11 RFRLQAKKWLNRTALRFREQaqwqEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPI 90
Cdd:PRK08279  42 VFEEAAARHPDRPALLFEDQ----SISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVALL 117
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  91 YATNTAKQVEYIVNDADIKILFVGdqEQFDQVYQIANNCPQLMKIVAMKANMHLRDLPNacyWEDFLNVVPNEAEFEKRL 170
Cdd:PRK08279 118 NTQQRGAVLAHSLNLVDAKHLIVG--EELVEAFEEARADLARPPRLWVAGGDTLDDPEG---YEDLAAAAAGAPTTNPAS 192
                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 171 NSK-QLSDLFTLIYTSGTTGEPKGV-------MLDYANLAHQLNAhdlalnvNEDDVSLSFLPLSH 228
Cdd:PRK08279 193 RSGvTAKDTAFYIYTSGTTGLPKAAvmshmrwLKAMGGFGGLLRL-------TPDDVLYCCLPLYH 251
PRK12476 PRK12476
putative fatty-acid--CoA ligase; Provisional
385-500 1.92e-06

putative fatty-acid--CoA ligase; Provisional


Pssm-ID: 171527 [Multi-domain]  Cd Length: 612  Bit Score: 50.90  E-value: 1.92e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 385 NPNSiGTLMPNAEVkigeeNEILVRGGMVMRGYYKKPEETAKAF----------------TEDG--FLRTGDVG-EMDsc 445
Cdd:PRK12476 417 DPDT-GAELPDGEV-----GEIWLHGDNIGRGYWGRPEETERTFgaklqsrlaegshadgAADDgtWLRTGDLGvYLD-- 488
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 446 GNLFITDRLKELMkTSNGKYIAPQYIEgkigkdkfieqiAVIADA-----KKYVSALIVP 500
Cdd:PRK12476 489 GELYITGRIADLI-VIDGRNHYPQDIE------------ATVAEAspmvrRGYVTAFTVP 535
PRK07788 PRK07788
acyl-CoA synthetase; Validated
367-472 2.75e-06

acyl-CoA synthetase; Validated


Pssm-ID: 236097 [Multi-domain]  Cd Length: 549  Bit Score: 50.31  E-value: 2.75e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 367 YGMTE-TTATVSCWQDKGFNPNSIGTLMPNAEVKIGEEN----------EILVRGGMVMRGYY--KKPEetakafTEDGF 433
Cdd:PRK07788 355 YGSTEvAFATIATPEDLAEAPGTVGRPPKGVTVKILDENgnevprgvvgRIFVGNGFPFEGYTdgRDKQ------IIDGL 428
                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 544685990 434 LRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIE 472
Cdd:PRK07788 429 LSSGDVGYFDEDGLLFVDGRDDD-MIVSGGENVFPAEVE 466
FACL_like_1 cd05910
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ...
181-465 3.57e-06

Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.


Pssm-ID: 341236 [Multi-domain]  Cd Length: 457  Bit Score: 49.77  E-value: 3.57e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 181 LIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHIFERAW-----IAYVFHRGATNCyleDTKHVR 255
Cdd:cd05910   90 ILFTSGSTGTPKGVVYRHGTFAAQIDALRQLYGIRPGEVDLATFPLFALFGPALgltsvIPDMDPTRPARA---DPQKLV 166
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 256 DALTTLKPTVMCAVPrfyekiytAVWDKVEkaplhrralfnwaiRVGEQHyQIEQPSQWLRLQY-ALADKLVLTKLRALL 334
Cdd:cd05910  167 GAIRQYGVSIVFGSP--------ALLERVA--------------RYCAQH-GITLPSLRRVLSAgAPVPIALAARLRKML 223
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 335 GGRIKMMPCGGAKlEA----SIGSffHSIginiklgygMTETTATVScwQDKGfnpNSIGTLMPNAEVKI---------- 400
Cdd:cd05910  224 SDEAEILTPYGAT-EAlpvsSIGS--REL---------LATTTAATS--GGAG---TCVGRPIPGVRVRIieiddepiae 286
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 401 ---------GEENEILVRGGMVMRGYYKKPEETAKAFTED---GFL-RTGDVGEMDSCGNLFITDRLKELMKTSNGKY 465
Cdd:cd05910  287 wddtlelprGEIGEITVTGPTVTPTYVNRPVATALAKIDDnseGFWhRMGDLGYLDDEGRLWFCGRKAHRVITTGGTL 364
PRK06334 PRK06334
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
170-459 4.06e-06

long chain fatty acid--[acyl-carrier-protein] ligase; Validated


Pssm-ID: 180533 [Multi-domain]  Cd Length: 539  Bit Score: 49.81  E-value: 4.06e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 170 LNSKQLSDLFTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHiferawiAYVFHrgatNCYLE 249
Cdd:PRK06334 177 VSDKDPEDVAVILFTSGTEKLPKGVPLTHANLLANQRACLKFFSPKEDDVMMSFLPPFH-------AYGFN----SCTLF 245
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 250 dtkhvrdALTTLKPTVMCAVPRFYEKIYTAVwDKVEKAPLHRRALFNWAIrvgeqhyqieqpsqwlrLQYALADKLVLTK 329
Cdd:PRK06334 246 -------PLLSGVPVVFAYNPLYPKKIVEMI-DEAKVTFLGSTPVFFDYI-----------------LKTAKKQESCLPS 300
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 330 LR-ALLGGRIkmmpCGGAKLEASIGSFFHsigINIKLGYGMTETTATVSC-WQDKGFNPNSIGTLMPNAEVKI------- 400
Cdd:PRK06334 301 LRfVVIGGDA----FKDSLYQEALKTFPH---IQLRQGYGTTECSPVITInTVNSPKHESCVGMPIRGMDVLIvseetkv 373
                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 544685990 401 ----GEENEILVRGGMVMRGYYKkpEETAKAFTE---DGFLRTGDVGEMDSCGNLFITDRLKELMK 459
Cdd:PRK06334 374 pvssGETGLVLTRGTSLFSGYLG--EDFGQGFVElggETWYVTGDLGYVDRHGELFLKGRLSRFVK 437
PRK07769 PRK07769
long-chain-fatty-acid--CoA ligase; Validated
34-500 4.12e-06

long-chain-fatty-acid--CoA ligase; Validated


Pssm-ID: 181109 [Multi-domain]  Cd Length: 631  Bit Score: 49.73  E-value: 4.12e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  34 QEMSWQTFQQEIdRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE---YIVNDADIKI 110
Cdd:PRK07769  54 RDLTWSQFGARN-RAVGARLQQVTKPGDRVAILAPQNLDYLIAFFGALYAGRIAVPLFDPAEPGHVGrlhAVLDDCTPSA 132
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 111 LFVGDqEQFDQVYQIANNCP--QLMKIVAMKAnmhlrdLPNACyWEDFLNVVPNEaefekrlnskqlSDLFTLIYTSGTT 188
Cdd:PRK07769 133 ILTTT-DSAEGVRKFFRARPakERPRVIAVDA------VPDEV-GATWVPPEANE------------DTIAYLQYTSGST 192
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 189 GEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSHifERAWIAYVFHR--GATNCYLEDTKHVRDALTTLKPtvM 266
Cdd:PRK07769 193 RIPAGVQITHLNLPTNVLQVIDALEGQEGDRGVSWLPFFH--DMGLITVLLPAllGHYITFMSPAAFVRRPGRWIRE--L 268
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 267 CAVPRFYEKIYTAvwdkvekAPlhrralfNWAI---------RVGEQhyqieqpsqwlrlqyaladKLVLTKLRALLGGR 337
Cdd:PRK07769 269 ARKPGGTGGTFSA-------AP-------NFAFehaaarglpKDGEP-------------------PLDLSNVKGLLNGS 315
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 338 IKMMPcggakleASIGSFFHSIG------INIKLGYGMTETTATVSC--WQDKgfnPNSI---------GTLM------P 394
Cdd:PRK07769 316 EPVSP-------ASMRKFNEAFApyglppTAIKPSYGMAEATLFVSTtpMDEE---PTVIyvdrdelnaGRFVevpadaP 385
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 395 NA------------------------EVKIGEENEILVRGGMVMRGYYKKPEETAKAF----------------TEDG-F 433
Cdd:PRK07769 386 NAvaqvsagkvgvsewavivdpetasELPDGQIGEIWLHGNNIGTGYWGKPEETAATFqnilksrlseshaegaPDDAlW 465
                        490       500       510       520       530       540
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 434 LRTGDVGE-MDscGNLFITDRLKELMkTSNGKYIAPQYIEgkigkdkFIEQIAVIADAKKYVSALIVP 500
Cdd:PRK07769 466 VRTGDYGVyFD--GELYITGRVKDLV-IIDGRNHYPQDLE-------YTAQEATKALRTGYVAAFSVP 523
PRK13388 PRK13388
acyl-CoA synthetase; Provisional
22-488 1.42e-05

acyl-CoA synthetase; Provisional


Pssm-ID: 237374 [Multi-domain]  Cd Length: 540  Bit Score: 48.10  E-value: 1.42e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  22 RTALRFREQaQWqemSWQTFQQEIDRFSYALIAQHI-DIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYATNTAKQVE 100
Cdd:PRK13388  17 TIAVRYGDR-TW---TWREVLAEAAARAAALIALADpDRPLHVGVLLGNTPEMLFWLAAAALGGYVLVGLNTTRRGAALA 92
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 101 YIVNDADIKILfVGDQEqfdqvyqianNCPQLMKIvamkanmhlrDLPNacywEDFLNVvpNEAEFEKRLNSKQ------ 174
Cdd:PRK13388  93 ADIRRADCQLL-VTDAE----------HRPLLDGL----------DLPG----VRVLDV--DTPAYAELVAAAGaltphr 145
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 175 ---LSDLFTLIYTSGTTGEPKGVMLDYANLAhqLNAHDLA--LNVNEDDVSLSFLPLSH---IFerAWIAYVFHRGATnc 246
Cdd:PRK13388 146 evdAMDPFMLIFTSGTTGAPKAVRCSHGRLA--FAGRALTerFGLTRDDVCYVSMPLFHsnaVM--AGWAPAVASGAA-- 219
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 247 yledtkhvrdalttlkptvMCAVPRFYEkiyTAVWDKVekaplhRR---ALFNWairVGEQHYQI----EQPSQwlrlqy 319
Cdd:PRK13388 220 -------------------VALPAKFSA---SGFLDDV------RRygaTYFNY---VGKPLAYIlatpERPDD------ 262
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 320 alADklvlTKLRALLGGrikmmpcggaklEAS---IGSFFHSIGINIKLGYGMTETTATVScwQDKGFNPNSIG------ 390
Cdd:PRK13388 263 --AD----NPLRVAFGN------------EASprdIAEFSRRFGCQVEDGYGSSEGAVIVV--REPGTPPGSIGrgapgv 322
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 391 ------TLMP-------------NAEVKIGEeneiLV--RGGMVMRGYYKKPEETAKAFtEDGFLRTGDVGEMDSCGNLF 449
Cdd:PRK13388 323 aiynpeTLTEcavarfdahgallNADEAIGE----LVntAGAGFFEGYYNNPEATAERM-RHGMYWSGDLAYRDADGWIY 397
                        490       500       510
                 ....*....|....*....|....*....|....*....
gi 544685990 450 ITDRLKELMKTsNGKYIAPQYIEGKIGKDKFIEQIAVIA 488
Cdd:PRK13388 398 FAGRTADWMRV-DGENLSAAPIERILLRHPAINRVAVYA 435
PRK05691 PRK05691
peptide synthase; Validated
15-487 2.42e-05

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 47.86  E-value: 2.42e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   15 QAKKWLNRTALrfreqaQWQEMS--WQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIADFGAMQARAVAVPIYA 92
Cdd:PRK05691 1140 QARQTPERIAL------VWDGGSldYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLDP 1213
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   93 TNTAKQVEYIVNDADIKILfVGDQEQFDQVYQIANNCPqlmkiVAMkANMHLRDLPNAcywedflnvVPNEAefekrLNS 172
Cdd:PRK05691 1214 DYPAERLAYMLADSGVELL-LTQSHLLERLPQAEGVSA-----IAL-DSLHLDSWPSQ---------APGLH-----LHG 1272
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  173 KQLSdlfTLIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLShiferawiayvFHRGATNCYLedtk 252
Cdd:PRK05691 1273 DNLA---YVIYTSGSTGQPKGVGNTHAALAERLQWMQATYALDDSDVLMQKAPIS-----------FDVSVWECFW---- 1334
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  253 hvrdALTTLKPTVMCAVPRfyekiytavwdkvekaplHR--RALFNWAIRVGEQHYQIEQPSQWLRLQYALADKlvLTKL 330
Cdd:PRK05691 1335 ----PLITGCRLVLAGPGE------------------HRdpQRIAELVQQYGVTTLHFVPPLLQLFIDEPLAAA--CTSL 1390
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  331 RALLggrikmmpCGGAKLEASIGSFFHSIGINIKLG--YGMTETTATVSCWQ----DKGFNPnsIGTLMPNA--EVKIGE 402
Cdd:PRK05691 1391 RRLF--------SGGEALPAELRNRVLQRLPQVQLHnrYGPTETAINVTHWQcqaeDGERSP--IGRPLGNVlcRVLDAE 1460
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  403 EN--------EILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGDVGEMDSCGNLFITDRLKELMKTsNGKYIA 467
Cdd:PRK05691 1461 LNllppgvagELCIGGAGLARGYLGRPALTAERFVPDPLgedgarlYRTGDRARWNADGALEYLGRLDQQVKL-RGFRVE 1539
                         490       500
                  ....*....|....*....|
gi 544685990  468 PQYIEGKIGKDKFIEQIAVI 487
Cdd:PRK05691 1540 PEEIQARLLAQPGVAQAAVL 1559
PRK07638 PRK07638
acyl-CoA synthetase; Validated
179-493 2.43e-05

acyl-CoA synthetase; Validated


Pssm-ID: 236071 [Multi-domain]  Cd Length: 487  Bit Score: 47.08  E-value: 2.43e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 179 FTLIYTSGTTGEPKGVMLDYANLAH--QLNAHDLALNvNEDDVSLSFLPLSHIFERAWIAyVFHRGATNCYLED--TKHV 254
Cdd:PRK07638 146 FYMGFTSGSTGKPKAFLRAQQSWLHsfDCNVHDFHMK-REDSVLIAGTLVHSLFLYGAIS-TLYVGQTVHLMRKfiPNQV 223
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 255 RDALTTLKPTVMCAVPRFYEKIYtavwdKVEKAPlhrralfnwairvgeqhyqiEQPsqwlrlqyalaDKLVLTklrall 334
Cdd:PRK07638 224 LDKLETENISVMYTVPTMLESLY-----KENRVI--------------------ENK-----------MKIISS------ 261
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 335 ggrikmmpcgGAKLEASIGSFFHSIGINIKLG--YGMTE---TTATVScwQDKGFNPNSIGTLMPNAEVKI--------- 400
Cdd:PRK07638 262 ----------GAKWEAEAKEKIKNIFPYAKLYefYGASElsfVTALVD--EESERRPNSVGRPFHNVQVRIcneageevq 329
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 401 -GEENEILVRGGMVMRGYyKKPEETAKAFTEDGFLRTGDVGEMDSCGNLFITDRLKElMKTSNGKYIAPQYIEGKIGKDK 479
Cdd:PRK07638 330 kGEIGTVYVKSPQFFMGY-IIGGVLARELNADGWMTVRDVGYEDEEGFIYIVGREKN-MILFGGINIFPEEIESVLHEHP 407
                        330
                 ....*....|....
gi 544685990 480 FIEQIAVIADAKKY 493
Cdd:PRK07638 408 AVDEIVVIGVPDSY 421
Ac_CoA_lig_AcsA TIGR02188
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ...
21-205 6.64e-05

acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.


Pssm-ID: 274022 [Multi-domain]  Cd Length: 626  Bit Score: 45.70  E-value: 6.64e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   21 NRTALRFR--EQAQWQEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIAdfgaMQA--R--AVAVPIYATN 94
Cdd:TIGR02188  72 DKVAIIWEgdEPGEVRKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIA----MLAcaRigAIHSVVFGGF 147
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990   95 TAKQVEYIVNDADIKILFVGDqEQF---------DQVYQIANNCPQLMK--IVAMKANMHL------RDLpnacYWEDFL 157
Cdd:TIGR02188 148 SAEALADRINDAGAKLVITAD-EGLrggkviplkAIVDEALEKCPVSVEhvLVVRRTGNPVvpwvegRDV----WWHDLM 222
                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....
gi 544685990  158 NVVPNEAEFEKrLNSKQLsdLFTLiYTSGTTGEPKGVM------LDYANLAHQL 205
Cdd:TIGR02188 223 AKASAYCEPEP-MDSEDP--LFIL-YTSGSTGKPKGVLhttggyLLYAAMTMKY 272
PRK13383 PRK13383
acyl-CoA synthetase; Provisional
327-487 8.82e-05

acyl-CoA synthetase; Provisional


Pssm-ID: 139531 [Multi-domain]  Cd Length: 516  Bit Score: 45.37  E-value: 8.82e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 327 LTKLRALLGGRIKMMPCGGAKLEASIGSFFHSIGINIKLGYGMTETTATVSCWqdkgfnPNSIGTLMPNAEVKIGEEN-- 404
Cdd:PRK13383 291 LPQLRVVMSSGDRLDPTLGQRFMDTYGDILYNGYGSTEVGIGALATPADLRDA------PETVGKPVAGCPVRILDRNnr 364
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 405 --------EILVRGGMVMRGYykkPEETAKAFTeDGFLRTGDVGEMDSCGNLFITDRlKELMKTSNGKYIAPQYIEGKIG 476
Cdd:PRK13383 365 pvgprvtgRIFVGGELAGTRY---TDGGGKAVV-DGMTSTGDMGYLDNAGRLFIVGR-EDDMIISGGENVYPRAVENALA 439
                        170
                 ....*....|.
gi 544685990 477 KDKFIEQIAVI 487
Cdd:PRK13383 440 AHPAVADNAVI 450
PRK07867 PRK07867
acyl-CoA synthetase; Validated
174-228 1.24e-04

acyl-CoA synthetase; Validated


Pssm-ID: 236120 [Multi-domain]  Cd Length: 529  Bit Score: 45.06  E-value: 1.24e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 544685990 174 QLSDLFTLIYTSGTTGEPKGVMLDYANLA---HQLNAHdlaLNVNEDDVSLSFLPLSH 228
Cdd:PRK07867 150 DPDDLFMLIFTSGTSGDPKAVRCTHRKVAsagVMLAQR---FGLGPDDVCYVSMPLFH 204
PRK13390 PRK13390
acyl-CoA synthetase; Provisional
417-516 2.37e-04

acyl-CoA synthetase; Provisional


Pssm-ID: 139538 [Multi-domain]  Cd Length: 501  Bit Score: 43.84  E-value: 2.37e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 417 YYKKPEETAKA-FTEDGFLRT-GDVGEMDSCGNLFITDRlKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIA----DA 490
Cdd:PRK13390 362 YLNDPEKTAAAqHPAHPFWTTvGDLGSVDEDGYLYLADR-KSFMIISGGVNIYPQETENALTMHPAVHDVAVIGvpdpEM 440
                         90       100
                 ....*....|....*....|....*...
gi 544685990 491 KKYVSALI--VPCFDSLEEYAKQLnIKY 516
Cdd:PRK13390 441 GEQVKAVIqlVEGIRGSDELAREL-IDY 467
ACS cd05966
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ...
43-219 3.57e-04

Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.


Pssm-ID: 341270 [Multi-domain]  Cd Length: 608  Bit Score: 43.32  E-value: 3.57e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  43 QEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIAdfgaMQA--R--AVAVPIYATNTAKQVEYIVNDADIKILFVGDQ-- 116
Cdd:cd05966   92 REVCRFANVLKSLGVKKGDRVAIYMPMIPELVIA----MLAcaRigAVHSVVFAGFSAESLADRINDAQCKLVITADGgy 167
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 117 --EQFDQVYQIAN----NCPQLMK-IVAMKAN-----MHLRDLpnacYWEDFLNVVPNEAEFEkRLNSKqlsD-LFTLiY 183
Cdd:cd05966  168 rgGKVIPLKEIVDealeKCPSVEKvLVVKRTGgevpmTEGRDL----WWHDLMAKQSPECEPE-WMDSE---DpLFIL-Y 238
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
gi 544685990 184 TSGTTGEPKGVM------LDYANLAHQlnahdLALNVNEDDV 219
Cdd:cd05966  239 TSGSTGKPKGVVhttggyLLYAATTFK-----YVFDYHPDDI 275
PRK00174 PRK00174
acetyl-CoA synthetase; Provisional
136-204 5.83e-04

acetyl-CoA synthetase; Provisional


Pssm-ID: 234677 [Multi-domain]  Cd Length: 637  Bit Score: 42.82  E-value: 5.83e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 544685990 136 VAMKANmhlRDLpnacYWEDFLNVVPNEAEFEkRLNSKQLsdLFTLiYTSGTTGEPKGVM------LDYANLAHQ 204
Cdd:PRK00174 216 VDWVEG---RDL----WWHELVAGASDECEPE-PMDAEDP--LFIL-YTSGSTGKPKGVLhttggyLVYAAMTMK 279
AACS cd05943
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ...
34-195 8.71e-04

Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.


Pssm-ID: 341265 [Multi-domain]  Cd Length: 629  Bit Score: 42.26  E-value: 8.71e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  34 QEMSWQTFQQEIDRFSYALIAQHIDIQDKIGIFANNMPRWTIA----------------DFGAmqaRAVAvpiyatNTAK 97
Cdd:cd05943   97 TEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAmlatasigaiwsscspDFGV---PGVL------DRFG 167
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  98 QVEyivndadIKILFVGDQEQF--------DQVYQIANNCPQLMKIVAM-----KANMHLRDLPNACYWEDFLNVVPN-E 163
Cdd:cd05943  168 QIE-------PKVLFAVDAYTYngkrhdvrEKVAELVKGLPSLLAVVVVpytvaAGQPDLSKIAKALTLEDFLATGAAgE 240
                        170       180       190
                 ....*....|....*....|....*....|..
gi 544685990 164 AEFEkRLNSKQLsdLFTLiYTSGTTGEPKGVM 195
Cdd:cd05943  241 LEFE-PLPFDHP--LYIL-YSSGTTGLPKCIV 268
PRK05691 PRK05691
peptide synthase; Validated
181-438 1.86e-03

peptide synthase; Validated


Pssm-ID: 235564 [Multi-domain]  Cd Length: 4334  Bit Score: 41.69  E-value: 1.86e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  181 LIYTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLShiFERAwiayvfhrgatncyledTKHVRDALTT 260
Cdd:PRK05691 2338 LIYTSGSTGKPKGVVVSHGEIAMHCQAVIERFGMRADDCELHFYSIN--FDAA-----------------SERLLVPLLC 2398
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  261 LKPTVMCAvprfyekiyTAVWDKVEKAPLHRralfnwairvgEQHYQI--------EQPSQWLRLQYA-LADKLVLTKLR 331
Cdd:PRK05691 2399 GARVVLRA---------QGQWGAEEICQLIR-----------EQQVSIlgftpsygSQLAQWLAGQGEqLPVRMCITGGE 2458
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990  332 ALLGGRIKMMPCGGAKleasiGSFFHSiginiklgYGMTETT-------ATVSCWQDKGFNPnsIGTLMPN--------- 395
Cdd:PRK05691 2459 ALTGEHLQRIRQAFAP-----QLFFNA--------YGPTETVvmplaclAPEQLEEGAASVP--IGRVVGArvayildad 2523
                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|.
gi 544685990  396 -AEVKIGEENEILVRGGMVMRGYYKKPEETAKAFTEDGF-------LRTGD 438
Cdd:PRK05691 2524 lALVPQGATGELYVGGAGLAQGYHDRPGLTAERFVADPFaadggrlYRTGD 2574
PRK08308 PRK08308
acyl-CoA synthetase; Validated
163-228 2.13e-03

acyl-CoA synthetase; Validated


Pssm-ID: 236231 [Multi-domain]  Cd Length: 414  Bit Score: 40.79  E-value: 2.13e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 544685990 163 EAEFEKRLNSKQLSDLFTLI-YTSGTTGEPKGVMLDYANLAHQLNAHDLALNVNEDDVSLSFLPLSH 228
Cdd:PRK08308  87 ESDFTKLEAVNYLAEEPSLLqYSSGTTGEPKLIRRSWTEIDREIEAYNEALNCEQDETPIVACPVTH 153
A_NRPS_alphaAR cd17647
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ...
361-447 4.26e-03

Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.


Pssm-ID: 341302 [Multi-domain]  Cd Length: 520  Bit Score: 39.81  E-value: 4.26e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544685990 361 INIKLGYGMTETTATVS------CWQDKGFNPN-----SIGTLMPNAEV------------KIGEENEILVRGGMVMRGY 417
Cdd:cd17647  250 VRIVNMYGTTETQRAVSyfevpsRSSDPTFLKNlkdvmPAGRGMLNVQLlvvnrndrtqicGIGEVGEIYVRAGGLAEGY 329
                         90       100       110
                 ....*....|....*....|....*....|
gi 544685990 418 YKKPEETAKAFTEDGFLRTGDVGEMDSCGN 447
Cdd:cd17647  330 RGLPELNKEKFVNNWFVEPDHWNYLDKDNN 359
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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