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Conserved domains on  [gi|544952977|ref|WP_021358822|]
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SAM-dependent methyltransferase [Clostridioides difficile]

Protein Classification

class I SAM-dependent methyltransferase( domain architecture ID 10616068)

class I SAM-dependent methyltransferase catalyzes the methylation of one or more specific substrates using S-adenosyl-L-methionine (SAM or AdoMet) as the methyl donor; similar to Homo sapiens methyltransferase-like protein 25B

CATH:  2.20.25.110
EC:  2.1.1.-
Gene Ontology:  GO:1904047|GO:0008168|GO:0032259|GO:0008757
PubMed:  12826405|12504684
SCOP:  3000118

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Methyltransf_32 pfam13679
Methyltransferase domain; This family appears to be a methyltransferase domain.
 158-295 3.27e-50

Methyltransferase domain; This family appears to be a methyltransferase domain.


:

Pssm-ID: 379330 [Multi-domain]  Cd Length: 138  Bit Score: 165.05  E-value: 3.27e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544952977  158 RKYNQIDHFVELIDNILKEIKDK-DCITILDCACGKSYLSFVLNFyikevLKKNCYFIGIDYSDVVIEASKNMAKNLGY- 235
Cdd:pfam13679   1 KKLHQVEHLAEFIAPLLKELLDEnGPITIVDHGAGKGYLGFILYY-----LKYGVRVYGIDTRAELVEKANALAQKLGFn 75

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 544952977  236 KNMSFIKEDLTNYTP---NRDVDLVISLHACDTATDMAIGLGIRAKSEAIVVVPCCHKELLGQ 295
Cdd:pfam13679  76 KRMSFLEGTIAGSTPvelPDRVDVVTALHACDTATDDALRFALAKQARAIVLVPCCYHELAEQ 138
 
Name Accession Description Interval E-value
Methyltransf_32 pfam13679
Methyltransferase domain; This family appears to be a methyltransferase domain.
 158-295 3.27e-50

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 379330 [Multi-domain]  Cd Length: 138  Bit Score: 165.05  E-value: 3.27e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544952977  158 RKYNQIDHFVELIDNILKEIKDK-DCITILDCACGKSYLSFVLNFyikevLKKNCYFIGIDYSDVVIEASKNMAKNLGY- 235
Cdd:pfam13679   1 KKLHQVEHLAEFIAPLLKELLDEnGPITIVDHGAGKGYLGFILYY-----LKYGVRVYGIDTRAELVEKANALAQKLGFn 75

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 544952977  236 KNMSFIKEDLTNYTP---NRDVDLVISLHACDTATDMAIGLGIRAKSEAIVVVPCCHKELLGQ 295
Cdd:pfam13679  76 KRMSFLEGTIAGSTPvelPDRVDVVTALHACDTATDDALRFALAKQARAIVLVPCCYHELAEQ 138
UbiE COG2226
Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; ...
 160-262 1.02e-06

Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 441828 [Multi-domain]  Cd Length: 143  Bit Score: 47.68  E-value: 1.02e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544952977 160 YNQIDHFVELIDNILKEIKDKDCITILDCACGKSYLSFVLnfyikevLKKNCYFIGIDYSDVVIEASKNMAKNLGYkNMS 239
Cdd:COG2226    1 FDRVAARYDGREALLAALGLRPGARVLDLGCGTGRLALAL-------AERGARVTGVDISPEMLELARERAAEAGL-NVE 72

                         90       100
                 ....*....|....*....|....*
gi 544952977 240 FIKEDLTNyTPNRD--VDLVISLHA 262
Cdd:COG2226   73 FVVGDAED-LPFPDgsFDLVISSFV 96
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 184-263 1.04e-06

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 47.04  E-value: 1.04e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544952977 184 TILDCACGKSYLSFVLnfyikeVLKKNCYFIGIDYSDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRD--VDLVISLH 261
Cdd:cd02440    1 RVLDLGCGTGALALAL------ASGPGARVTGVDISPVALELARKAAAALLADNVEVLKGDAEELPPEADesFDVIISDP 74


                 ..
gi 544952977 262 AC 263
Cdd:cd02440   75 PL 76
 
Name Accession Description Interval E-value
Methyltransf_32 pfam13679
Methyltransferase domain; This family appears to be a methyltransferase domain.
 158-295 3.27e-50

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 379330 [Multi-domain]  Cd Length: 138  Bit Score: 165.05  E-value: 3.27e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544952977  158 RKYNQIDHFVELIDNILKEIKDK-DCITILDCACGKSYLSFVLNFyikevLKKNCYFIGIDYSDVVIEASKNMAKNLGY- 235
Cdd:pfam13679   1 KKLHQVEHLAEFIAPLLKELLDEnGPITIVDHGAGKGYLGFILYY-----LKYGVRVYGIDTRAELVEKANALAQKLGFn 75

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 544952977  236 KNMSFIKEDLTNYTP---NRDVDLVISLHACDTATDMAIGLGIRAKSEAIVVVPCCHKELLGQ 295
Cdd:pfam13679  76 KRMSFLEGTIAGSTPvelPDRVDVVTALHACDTATDDALRFALAKQARAIVLVPCCYHELAEQ 138
Methyltransf_31 pfam13847
Methyltransferase domain; This family appears to have methyltransferase activity.
 183-275 1.19e-10

Methyltransferase domain; This family appears to have methyltransferase activity.


Pssm-ID: 463998 [Multi-domain]  Cd Length: 150  Bit Score: 59.35  E-value: 1.19e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544952977  183 ITILDCACGKSYLSFVLNfyikEVLKKNCYFIGIDYSDVVIEASKNMAKNLGYKNMSFIKED----LTNYTPNRdVDLVI 258
Cdd:pfam13847   5 MRVLDLGCGTGHLSFELA----EELGPNAEVVGIDISEEAIEKARENAQKLGFDNVEFEQGDieelPELLEDDK-FDVVI 79

                          90
                  ....*....|....*..
gi 544952977  259 SLHACDTATDMAIGLGI 275
Cdd:pfam13847  80 SNCVLNHIPDPDKVLQE 96
UbiE COG2226
Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; ...
 160-262 1.02e-06

Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 441828 [Multi-domain]  Cd Length: 143  Bit Score: 47.68  E-value: 1.02e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544952977 160 YNQIDHFVELIDNILKEIKDKDCITILDCACGKSYLSFVLnfyikevLKKNCYFIGIDYSDVVIEASKNMAKNLGYkNMS 239
Cdd:COG2226    1 FDRVAARYDGREALLAALGLRPGARVLDLGCGTGRLALAL-------AERGARVTGVDISPEMLELARERAAEAGL-NVE 72

                         90       100
                 ....*....|....*....|....*
gi 544952977 240 FIKEDLTNyTPNRD--VDLVISLHA 262
Cdd:COG2226   73 FVVGDAED-LPFPDgsFDLVISSFV 96
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 184-263 1.04e-06

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 47.04  E-value: 1.04e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544952977 184 TILDCACGKSYLSFVLnfyikeVLKKNCYFIGIDYSDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRD--VDLVISLH 261
Cdd:cd02440    1 RVLDLGCGTGALALAL------ASGPGARVTGVDISPVALELARKAAAALLADNVEVLKGDAEELPPEADesFDVIISDP 74


                 ..
gi 544952977 262 AC 263
Cdd:cd02440   75 PL 76
Methyltransf_25 pfam13649
Methyltransferase domain; This family appears to be a methyltransferase domain.
 185-262 2.08e-04

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 463945 [Multi-domain]  Cd Length: 96  Bit Score: 39.85  E-value: 2.08e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 544952977  185 ILDCACGKSYLSFVLnfyIKEVlkkNCYFIGIDYSDVVIEASKNMAKNLGYkNMSFIKEDLTNYT-PNRDVDLVISLHA 262
Cdd:pfam13649   1 VLDLGCGTGRLTLAL---ARRG---GARVTGVDLSPEMLERARERAAEAGL-NVEFVQGDAEDLPfPDGSFDLVVSSGV 72
UbiG COG2227
2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase [Coenzyme transport and ...
 170-261 3.70e-03

2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase [Coenzyme transport and metabolism]; 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase is part of the Pathway/BioSystem: Ubiquinone biosynthesis


Pssm-ID: 441829 [Multi-domain]  Cd Length: 126  Bit Score: 37.30  E-value: 3.70e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 544952977 170 IDNILKEIKDKDCiTILDCACGKSYLSFVLnfyikevLKKNCYFIGIDYSDVVIEASKNMAKNLgykNMSFIKEDLTNYT 249
Cdd:COG2227   14 LAALLARLLPAGG-RVLDVGCGTGRLALAL-------ARRGADVTGVDISPEALEIARERAAEL---NVDFVQGDLEDLP 82

                         90
                 ....*....|...
gi 544952977 250 -PNRDVDLVISLH 261
Cdd:COG2227   83 lEDGSFDLVICSE 95
Methyltransf_11 pfam08241
Methyltransferase domain; Members of this family are SAM dependent methyltransferases.
 186-262 8.87e-03

Methyltransferase domain; Members of this family are SAM dependent methyltransferases.


Pssm-ID: 462406 [Multi-domain]  Cd Length: 94  Bit Score: 35.33  E-value: 8.87e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 544952977  186 LDCACGKSYLSFVLnfyikevLKKNCYFIGIDYSDVVIEASKNMAKNLGYKnmsFIKEDLTNyTPNRD--VDLVISLHA 262
Cdd:pfam08241   1 LDVGCGTGLLTELL-------ARLGARVTGVDISPEMLELAREKAPREGLT---FVVGDAED-LPFPDnsFDLVLSSEV 68
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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