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Conserved domains on  [gi|556277336|ref|WP_023288626|]
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MULTISPECIES: MGMT family protein [Klebsiella]

Protein Classification

MGMT family protein( domain architecture ID 10790320)

MGMT (O-6-methylguanine DNA methyltransferase) family protein similar to MGMT, also called methylated-DNA--[protein]-cysteine S-methyltransferase, that repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme, resulting in its irreversible inactivation

CATH:  1.10.10.10
EC:  2.1.1.63
Gene Ontology:  GO:0006281|GO:0003824|GO:0003908|GO:0003677

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Atl1 COG3695
Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like ...
 5-101 7.93e-45

Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like DNA-binding domain [Transcription];


:

Pssm-ID: 442910  Cd Length: 104  Bit Score: 140.32  E-value: 7.93e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556277336   5 DTFPQRVWHIVASIPEGYVTTYGDVARLAGSPRAARQVGGVLKRLPEGSTLPWHRVVNRHGDISLT-GPDLQRQRQALLA 83
Cdd:COG3695    4 EEFYERVYEVVAQIPPGRVATYGDIAALAGLPRGARQVGRALRALPEGSDLPWHRVVNADGRLSPGhAGGAEEQRELLEA 83

                         90
                 ....*....|....*...
gi 556277336  84 EGVQVSGSGHIDLQHYRW 101
Cdd:COG3695   84 EGVPVVDDGRVDLKRYRW 101
 
Name Accession Description Interval E-value
Atl1 COG3695
Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like ...
 5-101 7.93e-45

Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like DNA-binding domain [Transcription];


Pssm-ID: 442910  Cd Length: 104  Bit Score: 140.32  E-value: 7.93e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556277336   5 DTFPQRVWHIVASIPEGYVTTYGDVARLAGSPRAARQVGGVLKRLPEGSTLPWHRVVNRHGDISLT-GPDLQRQRQALLA 83
Cdd:COG3695    4 EEFYERVYEVVAQIPPGRVATYGDIAALAGLPRGARQVGRALRALPEGSDLPWHRVVNADGRLSPGhAGGAEEQRELLEA 83

                         90
                 ....*....|....*...
gi 556277336  84 EGVQVSGSGHIDLQHYRW 101
Cdd:COG3695   84 EGVPVVDDGRVDLKRYRW 101
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
 7-86 2.82e-32

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 107.83  E-value: 2.82e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556277336    7 FPQRVWHIVASIPEGYVTTYGDVARLAGSPRAARQVGGVLKRLPEGSTLPWHRVVNRHGDISLTGPDLQRQRQALLAEGV 86
Cdd:pfam01035   2 FQRRVWEALRQIPYGKTTTYGEIAKLLGRPKAARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRALLELEGV 81
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
 7-85 1.24e-29

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 101.02  E-value: 1.24e-29
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 556277336   7 FPQRVWHIVASIPEGYVTTYGDVARLAGSPRAARQVGGVLKRLPEGSTLPWHRVVNRHGDISLTGPDLQRQRQALLAEG 85
Cdd:cd06445    1 FQRRVWEALRQIPYGEVTTYGQIAKLAGTPKAARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELLELEG 79
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
 7-81 8.06e-14

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 60.79  E-value: 8.06e-14
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 556277336    7 FPQRVWHIVASIPEGYVTTYGDVARLAGSPRAARQVGGVLKRLPEGSTLPWHRVVNRHGDISLTGPDLQRQRQAL 81
Cdd:TIGR00589   3 FQQKVWKALRTIPYGETKSYGQLAKAIGNPKAARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKEFLL 77
PRK00901 PRK00901
methylated-DNA--protein-cysteine methyltransferase; Provisional
 7-71 2.24e-10

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 234860 [Multi-domain]  Cd Length: 155  Bit Score: 53.90  E-value: 2.24e-10
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 556277336   7 FPQRVWHIVASIPEGYVTTYGDVARLAGSPRAARQVGGVLKRLPEGSTLPWHRVVNRHGdiSLTG 71
Cdd:PRK00901  74 FQKKVWKALQEIPYGETRSYKEIAVNIGNPKACRAVGLANNKNPIPIFIPCHRVIGANG--KLVG 136
 
Name Accession Description Interval E-value
Atl1 COG3695
Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like ...
 5-101 7.93e-45

Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like DNA-binding domain [Transcription];


Pssm-ID: 442910  Cd Length: 104  Bit Score: 140.32  E-value: 7.93e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556277336   5 DTFPQRVWHIVASIPEGYVTTYGDVARLAGSPRAARQVGGVLKRLPEGSTLPWHRVVNRHGDISLT-GPDLQRQRQALLA 83
Cdd:COG3695    4 EEFYERVYEVVAQIPPGRVATYGDIAALAGLPRGARQVGRALRALPEGSDLPWHRVVNADGRLSPGhAGGAEEQRELLEA 83

                         90
                 ....*....|....*...
gi 556277336  84 EGVQVSGSGHIDLQHYRW 101
Cdd:COG3695   84 EGVPVVDDGRVDLKRYRW 101
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
 7-86 2.82e-32

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 107.83  E-value: 2.82e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556277336    7 FPQRVWHIVASIPEGYVTTYGDVARLAGSPRAARQVGGVLKRLPEGSTLPWHRVVNRHGDISLTGPDLQRQRQALLAEGV 86
Cdd:pfam01035   2 FQRRVWEALRQIPYGKTTTYGEIAKLLGRPKAARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRALLELEGV 81
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
 7-85 1.24e-29

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 101.02  E-value: 1.24e-29
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 556277336   7 FPQRVWHIVASIPEGYVTTYGDVARLAGSPRAARQVGGVLKRLPEGSTLPWHRVVNRHGDISLTGPDLQRQRQALLAEG 85
Cdd:cd06445    1 FQRRVWEALRQIPYGEVTTYGQIAKLAGTPKAARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELLELEG 79
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 3-90 5.19e-15

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 66.05  E-value: 5.19e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556277336   3 PLDT----FPQRVWHIVASIPEGYVTTYGDVARLAGSPRAARQVGGVLKRLPegstLPW----HRVVNRHGdiSLTG--P 72
Cdd:COG0350   72 PLDLrgtpFQRRVWEALRKIPYGETVTYGELARAIGRPKAARAVGSACGANP----IPIiipcHRVIGADG--SLGGyaG 145

                         90
                 ....*....|....*....
gi 556277336  73 DLQRqRQALLA-EGVQVSG 90
Cdd:COG0350  146 GLER-KRALLElEGALAAA 163
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
 7-81 8.06e-14

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 60.79  E-value: 8.06e-14
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 556277336    7 FPQRVWHIVASIPEGYVTTYGDVARLAGSPRAARQVGGVLKRLPEGSTLPWHRVVNRHGDISLTGPDLQRQRQAL 81
Cdd:TIGR00589   3 FQQKVWKALRTIPYGETKSYGQLAKAIGNPKAARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKEFLL 77
PRK00901 PRK00901
methylated-DNA--protein-cysteine methyltransferase; Provisional
 7-71 2.24e-10

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 234860 [Multi-domain]  Cd Length: 155  Bit Score: 53.90  E-value: 2.24e-10
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 556277336   7 FPQRVWHIVASIPEGYVTTYGDVARLAGSPRAARQVGGVLKRLPEGSTLPWHRVVNRHGdiSLTG 71
Cdd:PRK00901  74 FQKKVWKALQEIPYGETRSYKEIAVNIGNPKACRAVGLANNKNPIPIFIPCHRVIGANG--KLVG 136
PRK10286 PRK10286
methylated-DNA--[protein]-cysteine S-methyltransferase;
7-85 4.47e-09

methylated-DNA--[protein]-cysteine S-methyltransferase;


Pssm-ID: 182355 [Multi-domain]  Cd Length: 171  Bit Score: 50.64  E-value: 4.47e-09
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 556277336   7 FPQRVWHIVASIPEGYVTTYGDVARLAGSPRAARQVGGVLKRLPEGSTLPWHRVVNRHGDISLTGPDLQRQRQALLAEG 85
Cdd:PRK10286  89 FQREVWQTLRTIPCGQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKEWLLRHEG 167
PRK03887 PRK03887
methylated-DNA--protein-cysteine methyltransferase; Provisional
 7-87 2.86e-08

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 167628 [Multi-domain]  Cd Length: 175  Bit Score: 48.58  E-value: 2.86e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556277336   7 FPQRVWH-IVASIPEGYVTTYGDVAR-LAGSPRAarqVGGVLKRLPEGSTLPWHRVVNRHGdISLTGPDLQRQRQALLAE 84
Cdd:PRK03887  93 FERKVYEwLTKNVKRGEVITYGELAKaLNTSPRA---VGGAMKRNPYPIIVPCHRVVGRKN-PGLYTPKPEYKKFLLEVE 168


                 ...
gi 556277336  85 GVQ 87
Cdd:PRK03887 169 GVK 171
PRK15435 PRK15435
bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;
3-68 1.79e-07

bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;


Pssm-ID: 185333 [Multi-domain]  Cd Length: 353  Bit Score: 47.48  E-value: 1.79e-07
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556277336   3 PLD----TFPQRVWHIVASIPEGYVTTYGDVARLAGSPRAARQVGGVLKRLPEGSTLPWHRVVNRHGDIS 68
Cdd:PRK15435 263 PLDirgtAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGALS 332
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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