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Conserved domains on  [gi|556279750|ref|WP_023289076|]
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MULTISPECIES: PTS fructose-like transporter subunit IIB [Klebsiella]

Protein Classification

PTS fructose transporter subunit IIB( domain architecture ID 10787663)

PTS fructose transporter subunit IIB is part of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system that catalyzes the phosphorylation of fructose concomitantly with its translocation across the cell membrane

EC:  2.7.1.202
Gene Ontology:  GO:0090563|GO:0016310|GO:0022877|GO:0090582|GO:0016301|GO:0009401
SCOP:  4002238

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FrwB COG1445
Phosphotransferase system fructose-specific component IIB [Carbohydrate transport and ...
 4-98 1.58e-47

Phosphotransferase system fructose-specific component IIB [Carbohydrate transport and metabolism];


:

Pssm-ID: 441054 [Multi-domain]  Cd Length: 99  Bit Score: 147.18  E-value: 1.58e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750   4 IIAVTACPSGVAHTYMAAESLESAAKAKGWQVKVETQGSIGIENELTAEEIAAADMVILTKDIGIKfEERFAGKTIVRVN 83
Cdd:COG1445    2 IVAVTACPTGIAHTYMAAEALEKAAKELGHEIKVETQGSVGVENELTAEDIAAADAVILAADIEVD-MERFAGKPVIEVS 80

                         90
                 ....*....|....*
gi 556279750  84 ISDAVKRADAIMNKI 98
Cdd:COG1445   81 VKDAIKDPEALIEKA 95
 
Name Accession Description Interval E-value
FrwB COG1445
Phosphotransferase system fructose-specific component IIB [Carbohydrate transport and ...
 4-98 1.58e-47

Phosphotransferase system fructose-specific component IIB [Carbohydrate transport and metabolism];


Pssm-ID: 441054 [Multi-domain]  Cd Length: 99  Bit Score: 147.18  E-value: 1.58e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750   4 IIAVTACPSGVAHTYMAAESLESAAKAKGWQVKVETQGSIGIENELTAEEIAAADMVILTKDIGIKfEERFAGKTIVRVN 83
Cdd:COG1445    2 IVAVTACPTGIAHTYMAAEALEKAAKELGHEIKVETQGSVGVENELTAEDIAAADAVILAADIEVD-MERFAGKPVIEVS 80

                         90
                 ....*....|....*
gi 556279750  84 ISDAVKRADAIMNKI 98
Cdd:COG1445   81 VKDAIKDPEALIEKA 95
PRK10474 PRK10474
PTS fructose-like transporter subunit IIB;
19-106 7.71e-46

PTS fructose-like transporter subunit IIB;


Pssm-ID: 170468  Cd Length: 88  Bit Score: 142.33  E-value: 7.71e-46
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750  19 MAAESLESAAKAKGWQVKVETQGSIGIENELTAEEIAAADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMNKI 98
Cdd:PRK10474   1 MAAEALESAAKAKGWEVKVETQGSIGLENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKI 80


                 ....*...
gi 556279750  99 DSHLSQRA 106
Cdd:PRK10474  81 EAHLAQTA 88
FRU TIGR00829
PTS system, fructose-specific, IIB component; Bacterial PTS transporters transport and ...
 5-89 2.42e-42

PTS system, fructose-specific, IIB component; Bacterial PTS transporters transport and concomitantly phosphorylate their sugar substrates, and typically consist of multiple subunits or protein domains. The Fru family is a large and complex family which includes several sequenced fructose and mannitol-specific permeases as well as several PTS components of unknown specificities. The fructose components of this family phosphorylate fructose on the 1-position. The Fru family PTS systems typically have 3 domains, IIA, IIB and IIC, which may be found as 1 or more proteins. The fructose and mannitol transporters form separate phylogenetic clusters in this family. This family is specific for the IIB domain of the fructose PTS transporters. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids, Signal transduction, PTS]


Pssm-ID: 129909 [Multi-domain]  Cd Length: 85  Bit Score: 133.37  E-value: 2.42e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750    5 IAVTACPSGVAHTYMAAESLESAAKAKGWQVKVETQGSIGIENELTAEEIAAADMVILTKDIGIKFEERFAGKTIVRVNI 84
Cdd:TIGR00829   1 VAVTACPTGIAHTFMAAEALEKAAKKRGWEVKVETQGSVGAQNALTAEDIAAADGVILAADREIDLSRRFAGKNVYETST 80


                  ....*
gi 556279750   85 SDAVK 89
Cdd:TIGR00829  81 GDAIK 85
PTS_IIB_fructose cd05569
PTS_IIB_fructose: subunit IIB of enzyme II (EII) of the fructose-specific phosphoenolpyruvate: ...
 4-98 7.28e-42

PTS_IIB_fructose: subunit IIB of enzyme II (EII) of the fructose-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII (also referred to as FruAB) is a fructose-specific permease made up of two proteins (FruA and FruB) each containing 3 domains. The FruA protein contains two tandem nonidentical IIB domains and a C-terminal IIC transmembrane domain. Both IIB domains of FruA are included in this alignment. The FruB protein (also referred to as diphosphoryl transfer protein) contains a IIA domain, a domain of unknown function, and an Hpr-like domain called FPr (fructose-inducible HPr). This familiy also includes the IIB domains of several fructose-like PTS permeases including the Frv permease encoded by the frvABXR operon, the Frw permease encoded by the frwACBD operon, the Frx permease encoded by the hrsA gene, and the Fry permease encoded by the fryABC (ypdDGH) operon. FruAB takes up exogenous fructose, releasing the 1-phosphate ester in to the cytoplasm in preparation for metabolism primarily via glycolysis. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include fructose, chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99911 [Multi-domain]  Cd Length: 96  Bit Score: 132.61  E-value: 7.28e-42
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750   4 IIAVTACPSGVAHTYMAAESLESAAKAKGWQVKVETQGSIGIENELTAEEIAAADMVILTKDIGIKfEERFAGKTIVRVN 83
Cdd:cd05569    1 IVAVTACPTGIAHTYMAAEALEKAAKKLGWEIKVETQGSLGIENELTAEDIAEADAVILAADVPVD-DERFAGKRVYEVS 79

                         90
                 ....*....|....*
gi 556279750  84 ISDAVKRADAIMNKI 98
Cdd:cd05569   80 VAEAIKDAEAVIERA 94
PTS_IIB pfam02302
PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar ...
 5-93 6.16e-20

PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. The lactose/cellobiose-specific family are one of four structurally and functionally distinct group IIB PTS system cytoplasmic enzymes. The fold of IIB cellobiose shows similar structure to mammalian tyrosine phosphatases. This family also contains the fructose specific IIB subunit.


Pssm-ID: 396744  Cd Length: 92  Bit Score: 76.99  E-value: 6.16e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750    5 IAVTACPSGVAHTYMAAESLESAAKAKGWqvkVETQGSIGIeNELTAEEIAA-ADMVILTKDIGIKFEERFAGKTIVRVN 83
Cdd:pfam02302   1 KILTACGAGMATSLMAAEALEKAAKELGI---VEAQGAAGV-NELTAEDIADdADVVILAPDVAVEDLARFAGKPVYVIP 76

                          90
                  ....*....|
gi 556279750   84 ISDAVKRADA 93
Cdd:pfam02302  77 VKDALGMKDA 86
 
Name Accession Description Interval E-value
FrwB COG1445
Phosphotransferase system fructose-specific component IIB [Carbohydrate transport and ...
 4-98 1.58e-47

Phosphotransferase system fructose-specific component IIB [Carbohydrate transport and metabolism];


Pssm-ID: 441054 [Multi-domain]  Cd Length: 99  Bit Score: 147.18  E-value: 1.58e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750   4 IIAVTACPSGVAHTYMAAESLESAAKAKGWQVKVETQGSIGIENELTAEEIAAADMVILTKDIGIKfEERFAGKTIVRVN 83
Cdd:COG1445    2 IVAVTACPTGIAHTYMAAEALEKAAKELGHEIKVETQGSVGVENELTAEDIAAADAVILAADIEVD-MERFAGKPVIEVS 80

                         90
                 ....*....|....*
gi 556279750  84 ISDAVKRADAIMNKI 98
Cdd:COG1445   81 VKDAIKDPEALIEKA 95
PRK10474 PRK10474
PTS fructose-like transporter subunit IIB;
19-106 7.71e-46

PTS fructose-like transporter subunit IIB;


Pssm-ID: 170468  Cd Length: 88  Bit Score: 142.33  E-value: 7.71e-46
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750  19 MAAESLESAAKAKGWQVKVETQGSIGIENELTAEEIAAADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMNKI 98
Cdd:PRK10474   1 MAAEALESAAKAKGWEVKVETQGSIGLENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKI 80


                 ....*...
gi 556279750  99 DSHLSQRA 106
Cdd:PRK10474  81 EAHLAQTA 88
FRU TIGR00829
PTS system, fructose-specific, IIB component; Bacterial PTS transporters transport and ...
 5-89 2.42e-42

PTS system, fructose-specific, IIB component; Bacterial PTS transporters transport and concomitantly phosphorylate their sugar substrates, and typically consist of multiple subunits or protein domains. The Fru family is a large and complex family which includes several sequenced fructose and mannitol-specific permeases as well as several PTS components of unknown specificities. The fructose components of this family phosphorylate fructose on the 1-position. The Fru family PTS systems typically have 3 domains, IIA, IIB and IIC, which may be found as 1 or more proteins. The fructose and mannitol transporters form separate phylogenetic clusters in this family. This family is specific for the IIB domain of the fructose PTS transporters. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids, Signal transduction, PTS]


Pssm-ID: 129909 [Multi-domain]  Cd Length: 85  Bit Score: 133.37  E-value: 2.42e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750    5 IAVTACPSGVAHTYMAAESLESAAKAKGWQVKVETQGSIGIENELTAEEIAAADMVILTKDIGIKFEERFAGKTIVRVNI 84
Cdd:TIGR00829   1 VAVTACPTGIAHTFMAAEALEKAAKKRGWEVKVETQGSVGAQNALTAEDIAAADGVILAADREIDLSRRFAGKNVYETST 80


                  ....*
gi 556279750   85 SDAVK 89
Cdd:TIGR00829  81 GDAIK 85
PTS_IIB_fructose cd05569
PTS_IIB_fructose: subunit IIB of enzyme II (EII) of the fructose-specific phosphoenolpyruvate: ...
 4-98 7.28e-42

PTS_IIB_fructose: subunit IIB of enzyme II (EII) of the fructose-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII (also referred to as FruAB) is a fructose-specific permease made up of two proteins (FruA and FruB) each containing 3 domains. The FruA protein contains two tandem nonidentical IIB domains and a C-terminal IIC transmembrane domain. Both IIB domains of FruA are included in this alignment. The FruB protein (also referred to as diphosphoryl transfer protein) contains a IIA domain, a domain of unknown function, and an Hpr-like domain called FPr (fructose-inducible HPr). This familiy also includes the IIB domains of several fructose-like PTS permeases including the Frv permease encoded by the frvABXR operon, the Frw permease encoded by the frwACBD operon, the Frx permease encoded by the hrsA gene, and the Fry permease encoded by the fryABC (ypdDGH) operon. FruAB takes up exogenous fructose, releasing the 1-phosphate ester in to the cytoplasm in preparation for metabolism primarily via glycolysis. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include fructose, chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99911 [Multi-domain]  Cd Length: 96  Bit Score: 132.61  E-value: 7.28e-42
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750   4 IIAVTACPSGVAHTYMAAESLESAAKAKGWQVKVETQGSIGIENELTAEEIAAADMVILTKDIGIKfEERFAGKTIVRVN 83
Cdd:cd05569    1 IVAVTACPTGIAHTYMAAEALEKAAKKLGWEIKVETQGSLGIENELTAEDIAEADAVILAADVPVD-DERFAGKRVYEVS 79

                         90
                 ....*....|....*
gi 556279750  84 ISDAVKRADAIMNKI 98
Cdd:cd05569   80 VAEAIKDAEAVIERA 94
PRK10427 PRK10427
PTS fructose-like transporter subunit IIB;
1-103 2.41e-29

PTS fructose-like transporter subunit IIB;


Pssm-ID: 182451  Cd Length: 114  Bit Score: 101.46  E-value: 2.41e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750   1 MTHIIAVTACPSGVAHTYMAAESLESAAKAKGWQVKVETQGSIGIENELTAEEIAAADMVILTKDIGIKFEERFAGKTIV 80
Cdd:PRK10427   2 MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVKIETQGALGTENRLTDEDIRRADVVLLITDIELAGAERFEHCRYV 81

                         90       100
                 ....*....|....*....|...
gi 556279750  81 RVNISDAVKRADAIMNKIDSHLS 103
Cdd:PRK10427  82 QCSIYAFLREPQRVMSAVRKVLS 104
PRK11404 PRK11404
PTS fructose-like transporter subunit IIBC;
4-98 2.90e-29

PTS fructose-like transporter subunit IIBC;


Pssm-ID: 183120 [Multi-domain]  Cd Length: 482  Bit Score: 108.81  E-value: 2.90e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750   4 IIAVTACPSGVAHTYMAAESLESAAKAKGWQVKVETQGSIGIENELTAEEIAAADMVILTKDIGIKFEE--RFAGKTIVR 81
Cdd:PRK11404   6 IVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGVENRLSSEEIAAADYVILATGRGLSGDDraRFAGKKVYE 85

                         90
                 ....*....|....*..
gi 556279750  82 VNISDAVKRADAIMNKI 98
Cdd:PRK11404  86 IAISQALKNIDQIFSEL 102
PRK10712 PRK10712
PTS system fructose-specific transporter subunits IIBC; Provisional
 4-90 7.97e-29

PTS system fructose-specific transporter subunits IIBC; Provisional


Pssm-ID: 236740 [Multi-domain]  Cd Length: 563  Bit Score: 108.29  E-value: 7.97e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750   4 IIAVTACPSGVAHTYMAAESLESAAKAKGWQVKVETQGSIGIENELTAEEIAAADMVILTKDIGIKFeERFAGKTIVRVN 83
Cdd:PRK10712 106 VVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAADLVIVAADIEVDL-AKFAGKPMYRTS 184


                 ....*..
gi 556279750  84 ISDAVKR 90
Cdd:PRK10712 185 TGLALKK 191
PRK09765 PRK09765
PTS system 2-O-a-mannosyl-D-glycerate specific transporter subunit IIABC; Provisional
 4-97 1.07e-28

PTS system 2-O-a-mannosyl-D-glycerate specific transporter subunit IIABC; Provisional


Pssm-ID: 182066 [Multi-domain]  Cd Length: 631  Bit Score: 107.90  E-value: 1.07e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750   4 IIAVTACPSGVAHTYMAAESLESAAKAKGWQVKVETQGSIGIENELTAEEIAAADMVILTKDIGIKFEERFAGKTIVRVN 83
Cdd:PRK09765 166 IVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVP 245

                         90
                 ....*....|....
gi 556279750  84 ISDAVKRADAIMNK 97
Cdd:PRK09765 246 VAEPIKHAEALIQQ 259
PTS_IIB pfam02302
PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar ...
 5-93 6.16e-20

PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. The lactose/cellobiose-specific family are one of four structurally and functionally distinct group IIB PTS system cytoplasmic enzymes. The fold of IIB cellobiose shows similar structure to mammalian tyrosine phosphatases. This family also contains the fructose specific IIB subunit.


Pssm-ID: 396744  Cd Length: 92  Bit Score: 76.99  E-value: 6.16e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750    5 IAVTACPSGVAHTYMAAESLESAAKAKGWqvkVETQGSIGIeNELTAEEIAA-ADMVILTKDIGIKFEERFAGKTIVRVN 83
Cdd:pfam02302   1 KILTACGAGMATSLMAAEALEKAAKELGI---VEAQGAAGV-NELTAEDIADdADVVILAPDVAVEDLARFAGKPVYVIP 76

                          90
                  ....*....|
gi 556279750   84 ISDAVKRADA 93
Cdd:pfam02302  77 VKDALGMKDA 86
PTS_IIB cd00133
PTS_IIB: subunit IIB of enzyme II (EII) is the central energy-coupling domain of the ...
 5-98 9.56e-16

PTS_IIB: subunit IIB of enzyme II (EII) is the central energy-coupling domain of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In the multienzyme PTS complex, EII is a carbohydrate-specific permease consisting of two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system. The PTS is found only in bacteria, where it catalyzes the transport and phosphorylation of numerous monosaccharides, disaccharides, polyols, amino sugars, and other sugar derivatives. The four proteins (domains) forming the PTS phosphorylation cascade (EI, HPr, EIIA, and EIIB), can phosphorylate or interact with numerous non-PTS proteins thereby regulating their activity.


Pssm-ID: 99904  Cd Length: 84  Bit Score: 66.13  E-value: 9.56e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750   5 IAVTACPSGVAHTYMAAESLESAAKAKGWQVKVETQGSIGIENeltaeeIAAADMVILTKDIgikfEERFAGKTIVRVNI 84
Cdd:cd00133    1 KILVVCGSGIGSSSMLAEKLEKAAKELGIEVKVEAQGLSEVID------LADADLIISTVPL----AARFLGKPVIVVSP 70

                         90
                 ....*....|....
gi 556279750  85 SDAVKRADAIMNKI 98
Cdd:cd00133   71 LLNEKDGEKILEKL 84
FrwC COG1299
Phosphotransferase system, fructose-specific IIC component [Carbohydrate transport and ...
 4-94 1.37e-12

Phosphotransferase system, fructose-specific IIC component [Carbohydrate transport and metabolism];


Pssm-ID: 440910 [Multi-domain]  Cd Length: 469  Bit Score: 61.83  E-value: 1.37e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556279750   4 IIAVTACPSGVAHTYMAAESLESAAKAKGWQVKVETQGSIGIENELTAEEIAAADMVILTKDIGIKFEERFAGKTIVRVN 83
Cdd:COG1299   10 VVATTTAGATAAAAAAAAAAAAAAAAGGVIVVVVGGGGGGGGNLLTTEEEAAAAAIIIAAADDVEDRFFGKKGKVVVVVA 89

                         90
                 ....*....|.
gi 556279750  84 ISDAVKRADAI 94
Cdd:COG1299   90 GGAAKKELLII 100
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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