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Conserved domains on  [gi|556283776|ref|WP_023289784|]
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MULTISPECIES: thiaminase II [Klebsiella]

Protein Classification

TenA family protein( domain architecture ID 14443675)

TenA family protein such as Bacillus halodurans aminopyrimidine aminohydrolase, which catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, as part of the thiamine salvage pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 21-223 6.69e-110

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


:

Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 314.07  E-value: 6.69e-110
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  21 DWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAILNEVPLHVGYCAQWG 100
Cdd:cd19367    2 IWKAYTNHPFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHALAAYKAPTLEDIRAAAAILLAILEEMELHVKYCAEWG 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776 101 ISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPATRLEDNPYASWIRNYGDEGYLQG 180
Cdd:cd19367   82 ISEEELEATPESPATLAYTRYVLDVGLSGDLLDLLVALAPCLLGYGEIGLRLAADPATKLEGNPYWSWIETYASDEYQEA 161

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 556283776 181 VSAALALLETVWQQRGSEARLAGLSDIFTTATRLEAQFWQMGL 223
Cdd:cd19367  162 VREGIELLEKLAAERPSPARLEELVKIFATATRLEIGFWDMGL 204
 
Name Accession Description Interval E-value
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 21-223 6.69e-110

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 314.07  E-value: 6.69e-110
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  21 DWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAILNEVPLHVGYCAQWG 100
Cdd:cd19367    2 IWKAYTNHPFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHALAAYKAPTLEDIRAAAAILLAILEEMELHVKYCAEWG 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776 101 ISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPATRLEDNPYASWIRNYGDEGYLQG 180
Cdd:cd19367   82 ISEEELEATPESPATLAYTRYVLDVGLSGDLLDLLVALAPCLLGYGEIGLRLAADPATKLEGNPYWSWIETYASDEYQEA 161

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 556283776 181 VSAALALLETVWQQRGSEARLAGLSDIFTTATRLEAQFWQMGL 223
Cdd:cd19367  162 VREGIELLEKLAAERPSPARLEELVKIFATATRLEIGFWDMGL 204
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 9-227 2.18e-83

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 247.49  E-value: 2.18e-83
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776   9 GLYGRLRQLAAADWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAILN- 87
Cdd:COG0819    2 SFSERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLEv 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  88 EVPLHVGYCAQWGISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPatRLEDNPYAS 167
Cdd:COG0819   82 ELALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAERP--LPPDHPYAE 159

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776 168 WIRNYGDEGYLQGVSAALALLETVWqQRGSEARLAGLSDIFTTATRLEAQFWQMGLNAAA 227
Cdd:COG0819  160 WIETYASEEFQELVEWLIALLDRLA-ATASEAERERLEEAFRTASRLEYAFWDMAYRLER 218
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 22-225 1.06e-44

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 148.66  E-value: 1.06e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776   22 WQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMR-AAAASMNAILNEVPLHVGYCAQWG 100
Cdd:pfam03070   8 WKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRReWENRIVDHDGNEIELHLRLAEALG 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  101 ISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPaTRLEDNPYASWIRNYGDEGYLQG 180
Cdd:pfam03070  88 LSREDLSAYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGEKI-RALEGPEYYEWVKTYASEYFRAA 166

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 556283776  181 VSAALALLETVWQQrGSEARLAGLSDIFTTATRLEAQFWQMGLNA 225
Cdd:pfam03070 167 VEEAKRLLDRLLAY-VSDEQLEELQEIFLKALEFEYSFWTMALDA 210
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 18-223 1.36e-37

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 130.39  E-value: 1.36e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776   18 AAADWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAILN-EVPLHVGYC 96
Cdd:TIGR04306   1 VKPIWDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVEtEMGQHKRLA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776   97 AQWGISESEM--AAQPeAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPaTRLEDNPYASWIRNYGD 174
Cdd:TIGR04306  81 EVLGISDEEYfqKIKP-GPALRSYTSYMYDVSRRGNWQELVAALLPCLWGYGEAATKLKGKH-TAPEHSVYHKWIETYSS 158

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 556283776  175 EGYLQGVSAALALLETVwQQRGSEARLAGLSDIFTTATRLEAQFWQMGL 223
Cdd:TIGR04306 159 SWFREAVNEGENLLNHL-AETSSPEELEKLKTIFAESCEYEYNFWTMAY 206
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
13-227 3.43e-25

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 102.94  E-value: 3.43e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  13 RLRQLAAADWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLvSKLRTLPEMRA--AAASMNAILNEVP 90
Cdd:PRK14713 323 ALWQASGPIREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARL-AALAPDPAEQVfwAQSAQACLEVESE 401

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  91 LHVGYCA--QWGISESemaaqpeaPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGlLHHPATRLEDNPYASW 168
Cdd:PRK14713 402 LHRSWLGdrDADTAPS--------PVTLAYTDFLLARAAGGSYAVGAAAVLPCFWLYAEVGAE-LHARAGNPDDHPYAEW 472

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 556283776 169 IRNYGDEGYLQGVSAALALLETVWQQrGSEARLAGLSDIFTTATRLEAQFWQMGLNAAA 227
Cdd:PRK14713 473 LQTYADPEFAAATRRAIAFVDRAFRA-ASPAERAAMARAFLTACRYELEFFDQARRRAG 530
 
Name Accession Description Interval E-value
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 21-223 6.69e-110

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 314.07  E-value: 6.69e-110
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  21 DWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAILNEVPLHVGYCAQWG 100
Cdd:cd19367    2 IWKAYTNHPFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHALAAYKAPTLEDIRAAAAILLAILEEMELHVKYCAEWG 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776 101 ISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPATRLEDNPYASWIRNYGDEGYLQG 180
Cdd:cd19367   82 ISEEELEATPESPATLAYTRYVLDVGLSGDLLDLLVALAPCLLGYGEIGLRLAADPATKLEGNPYWSWIETYASDEYQEA 161

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 556283776 181 VSAALALLETVWQQRGSEARLAGLSDIFTTATRLEAQFWQMGL 223
Cdd:cd19367  162 VREGIELLEKLAAERPSPARLEELVKIFATATRLEIGFWDMGL 204
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 9-227 2.18e-83

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 247.49  E-value: 2.18e-83
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776   9 GLYGRLRQLAAADWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAILN- 87
Cdd:COG0819    2 SFSERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLEv 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  88 EVPLHVGYCAQWGISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPatRLEDNPYAS 167
Cdd:COG0819   82 ELALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAERP--LPPDHPYAE 159

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776 168 WIRNYGDEGYLQGVSAALALLETVWqQRGSEARLAGLSDIFTTATRLEAQFWQMGLNAAA 227
Cdd:COG0819  160 WIETYASEEFQELVEWLIALLDRLA-ATASEAERERLEEAFRTASRLEYAFWDMAYRLER 218
TenA_C_BhTenA-like cd19366
TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to ...
 13-221 2.16e-62

TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus halodurans TenA which participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381701 [Multi-domain]  Cd Length: 213  Bit Score: 193.93  E-value: 2.16e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  13 RLRQLAAADWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAILN-EVPL 91
Cdd:cd19366    4 RLREKAKPIWEAGLEHPFVQGLGDGTLDKEKFKFYLKQDYLYLIDYARVFALGAAKADDLETMGRFAELLHGTLNtEMDL 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  92 HVGYCAQWGISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPATrLEDNPYASWIRN 171
Cdd:cd19366   84 HRQYAAEFGITEEELEATEPSPTTLAYTSYMLRTAQTGTLAELLAALLPCAWGYAEIGKRLAEQGGA-LEHNPYREWIEM 162

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|
gi 556283776 172 YGDEGYLQGVSAALALLETVWQQRgSEARLAGLSDIFTTATRLEAQFWQM 221
Cdd:cd19366  163 YSSDEFTELADWLIDLLDELAEGK-SEAELERLEEIFLTSSRYEYMFWDM 211
TenA_C_HP1287-like cd19361
TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins ...
 6-221 3.13e-54

TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Helicobacter pylori TenA (HP1287) protein which is thought to catalyze a salvage reaction in thiamin metabolism, however its pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. HP1287 may contribute to stomach colonization and persistence.


Pssm-ID: 381696 [Multi-domain]  Cd Length: 212  Bit Score: 173.15  E-value: 3.13e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776   6 FSQGLYGRLRQLaaadWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAI 85
Cdd:cd19361    1 LSERLYEAVEDI----WDSYYEHPFVQGIADGTLDIEKFRFYMIQDYLYLLDYAKVFALGVAKAKDEEVMRFFADLINAI 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  86 LN-EVPLHVGYCAQWGISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPATRLEDNP 164
Cdd:cd19361   77 LNeEMDIHRGYMKRLGITEEEIENTKPALDNLSYTSYMLSVAYEGGIAEILAAILSCSWSYEYIAKKLVERYPAALEHEF 156

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 556283776 165 YASWIRNYGDEGYLQGVSAALALLETVWQQRgSEARLAGLSDIFTTATRLEAQFWQM 221
Cdd:cd19361  157 YGEWVKGYSSEEYAEANQELIDLLDRLTEDI-SEEQIEKLEEIFVNCSRYELKFWDM 212
TenA_C-like cd19369
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 22-221 2.84e-50

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381704 [Multi-domain]  Cd Length: 202  Bit Score: 162.40  E-value: 2.84e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  22 WQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAIL-NEVPLHVGYCAQWG 100
Cdd:cd19369    3 WEQYLEHPFIKELGEGTLDKEKFKNYLIQDSLYLKEYAKVFAMGIYKSRTMKEMQFFYSSLSFVNeDETATRIKYLKEFG 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776 101 ISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPATRLEDNPYASWIRNYGDEGYLQG 180
Cdd:cd19369   83 LTDEDIEKIEPLPENKAYTDYMLGIAKTGDVKEILMAVLPCMLSYYYIFKELVKKYKDNLESNPYKDWIEDYASEEYAEY 162

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 556283776 181 VSAALALLETVWQQRgSEARLAGLSDIFTTATRLEAQFWQM 221
Cdd:cd19369  163 CKEWIDFADRLCENL-SEEEKEKLKEIFRKASLYELKFWDM 202
TenA_PqqC-like cd16099
TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone ...
 21-221 2.53e-45

TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone (PQQ) synthesis protein C; TenA proteins participate in thiamin metabolism and can be classified into two classes: TenA_C which has an active site Cys, and TenA_E which does not; TenA_E proteins often have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product amino-HMP (4-amino-5-amino-methyl-2-methylpyrimidine) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin. Bacillus subtilis TenA_C can hydrolyze amino-HMP to AMP and can catalyze the hydrolysis of thiamin. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal ThiD domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). Bacillus halodurans TenA_C participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. Helicobacter pylori TenA_C is also thought to catalyze a salvage reaction but the pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect; Pyrococcus furiosus TenA_E lacks appropriate surface charges for DNA interactions. This family also includes bacterial coenzyme pyrroloquinoline quinone (PQQ) synthesis protein C (PQQC), an oxidase involved in the final step of PQQ biosynthesis, and CADD, a Chlamydia protein that interacts with death receptors.


Pssm-ID: 381691 [Multi-domain]  Cd Length: 196  Bit Score: 149.81  E-value: 2.53e-45
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  21 DWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAILNEVPLHVGYCAQWG 100
Cdd:cd16099    1 IWDAILNHPFVQELAAGTLPREKFRYYLAQDYYYLKDFARALALAAAKAPDLELRTFLAELINVLDDELELHEKLLAELG 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776 101 ISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPatrLEDNPYASWIRNYGDEGYLQG 180
Cdd:cd16099   81 ISEEDLSEAEPNPATLAYTNHLLRVAARGTPAEGLAALLPCYWSYGEIGRRLAASL---PEHPPYRFWIDFYASDEYQEL 157

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 556283776 181 VSAALALLETVWQQrgSEARLAGLSDIFTTATRLEAQFWQM 221
Cdd:cd16099  158 VEELLQLLDQLAAA--GEEEKEELKEIFLTSLRYELMFWDA 196
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 22-225 1.06e-44

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 148.66  E-value: 1.06e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776   22 WQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMR-AAAASMNAILNEVPLHVGYCAQWG 100
Cdd:pfam03070   8 WKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRReWENRIVDHDGNEIELHLRLAEALG 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  101 ISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPaTRLEDNPYASWIRNYGDEGYLQG 180
Cdd:pfam03070  88 LSREDLSAYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGEKI-RALEGPEYYEWVKTYASEYFRAA 166

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 556283776  181 VSAALALLETVWQQrGSEARLAGLSDIFTTATRLEAQFWQMGLNA 225
Cdd:pfam03070 167 VEEAKRLLDRLLAY-VSDEQLEELQEIFLKALEFEYSFWTMALDA 210
TenA_C-like cd19365
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 27-219 2.07e-41

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381700 [Multi-domain]  Cd Length: 205  Bit Score: 139.95  E-value: 2.07e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  27 AHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRA-AAASMNAILNEVPLHVGYCAQWGISese 105
Cdd:cd19365   17 AHPFIRELADGTLPREKFRFYLAQDALYLRDYARALALLAARAPDPEEQVFfARFAAGAIEVERELHRSFLGEFGID--- 93

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776 106 mAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLhhpATRLEDNPYASWIRNYGDEGYLQGVSAAL 185
Cdd:cd19365   94 -AAAEPSPVTLAYTSFLLATAATGPYAVAVAAVLPCFWIYAEVGKRLA---AAASPNHPYQDWIDTYSDEEFAEAVRRAI 169

                        170       180       190
                 ....*....|....*....|....*....|....
gi 556283776 186 ALLETVWQQrGSEARLAGLSDIFTTATRLEAQFW 219
Cdd:cd19365  170 AIVDRLAAE-ASPEERARMLEAFLRASRLEWMFW 202
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 18-223 1.36e-37

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 130.39  E-value: 1.36e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776   18 AAADWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAILN-EVPLHVGYC 96
Cdd:TIGR04306   1 VKPIWDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVEtEMGQHKRLA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776   97 AQWGISESEM--AAQPeAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPaTRLEDNPYASWIRNYGD 174
Cdd:TIGR04306  81 EVLGISDEEYfqKIKP-GPALRSYTSYMYDVSRRGNWQELVAALLPCLWGYGEAATKLKGKH-TAPEHSVYHKWIETYSS 158

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 556283776  175 EGYLQGVSAALALLETVwQQRGSEARLAGLSDIFTTATRLEAQFWQMGL 223
Cdd:TIGR04306 159 SWFREAVNEGENLLNHL-AETSSPEELEKLKTIFAESCEYEYNFWTMAY 206
TenA_C_SaTenA-like cd19360
TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins ...
 6-221 3.15e-36

TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Staphylococcus aureus TenA (SaTenA) which plays two essential roles in thiamin metabolism: in the deamination of aminopyrimidine to HMP, and in hydrolyzing thiamin into HMP and 5-(2-hydroxyethyl)4-methylthiazole (THZ). It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. SaTenA is then also a putative transcriptional regulator controlling the secretion of extracellular proteases such as subtilisin-type proteases in bacteria. This family includes mostly uncharacterized TenA like proteins.


Pssm-ID: 381695 [Multi-domain]  Cd Length: 211  Bit Score: 126.93  E-value: 3.15e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776   6 FSQglygRLRQLAAADWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAI 85
Cdd:cd19360    1 FSE----ELREEAQPILEAIYAHPFVQGIAAGELPKEALIHYVQQDYEYLNAFLKVYALAIAKSDTREDMRFFLEQIGFI 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  86 LN-EVPLHVGYCAQWGISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPATRlEDNP 164
Cdd:cd19360   77 LNgESHAHQNLCEVAGVDYEELQGAPWAPTADHYIKHMYYAARTGDLGDILAALLPCPWTYVELAKRLIEEGKPT-PDNP 155

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 556283776 165 YASWIRNYGDEGYLQGVSAALALLETvWQQRGSEARLAGLSDIFTTATRLEAQFWQM 221
Cdd:cd19360  156 FYEWIDFYADDEMDGLTDQLFARLDR-LAEKASEEERERAKQAFLKSCQLEWRFWEM 211
TenA_C_BsTenA-like cd19364
TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the ...
 13-221 2.37e-34

TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus subtilis TenA which has been shown to be a thiaminase II, catalyzing the hydrolysis of thiamine into HMP and 5-(2-hydroxyethyl)-4-methylthiazole (THZ), within thiamine metabolism. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381699 [Multi-domain]  Cd Length: 212  Bit Score: 121.91  E-value: 2.37e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  13 RLRQLAAADWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAILN-EVPL 91
Cdd:cd19364    4 ELREAADPLWQKSFEHPFIQGLADGTLPLETFRYYLIQDAYYLKHFAKLHALAAAKADDPAIKALLLEGAQGLAEgELAL 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  92 HVGYCAQWGISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLLHHPAtrleDNP-YASWIR 170
Cdd:cd19364   84 RETFFKELGITEEEIAQTPPAPTAYHYVSHMYRQLNEGSVAEAVAALLPCYWLYQEIGERLADAGS----PVPlYQRWID 159

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|.
gi 556283776 171 NYGDEGYLQGVSAALALLETVWQQRgSEARLAGLSDIFTTATRLEAQFWQM 221
Cdd:cd19364  160 TYASDEFTESVQQQIDLVDRLAEEA-SEEEREKMKQAFLISSYYELQFWEM 209
TenA_C_AtTH2-like cd19368
TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine ...
 28-218 6.52e-31

TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine requiring 2; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Arabidopsis thaliana TH2 is an orphan enzyme thiamin monophosphate phosphatase which has a haloacid dehalogenase (HAD) family domain fused to its TenA_C domain, it's TenA_C domain has thiamin salvage hydrolase activity against amino-HMP. This family includes mostly uncharacterized single-domain TenA_C- like proteins; some however have additional domains such as a HAD family domain or a kinase domain It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381703 [Multi-domain]  Cd Length: 210  Bit Score: 113.10  E-value: 6.52e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  28 HPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAILNEVPLHVGYCAQWGIsESEMA 107
Cdd:cd19368   19 HPFVVGLAAGNLPLDSFRHYISQDAHFLEAFARAYELAEAKADDDEDKKAIRELRKGVLEELKLHDSYAEEWGV-DLPKE 97

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776 108 AQPeAPETINYTRYVLDIGHSG--DALDLLVALMPCVAGYAEIGLGLLHHPATRlEDNPYASWIRNYGDEGYLQGVSAAL 185
Cdd:cd19368   98 VTP-DPATRKYTDFLLATASGKvkVAAYTLAAMAPCMRLYAFLGQELARALDDT-EDHPYKKWIDTYSSQEFEALALQLE 175

                        170       180       190
                 ....*....|....*....|....*....|...
gi 556283776 186 ALLETVwQQRGSEARLAGLSDIFTTATRLEAQF 218
Cdd:cd19368  176 DLLDKL-SASLTGEELEALEKLYRRAMKLEVEF 207
TenA_C_PH1161-like cd19363
uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA ...
 10-221 1.88e-28

uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes functionally uncharacterized TenA like proteins such as Pyrococcus horikoshii PH1161 protein.


Pssm-ID: 381698 [Multi-domain]  Cd Length: 210  Bit Score: 106.64  E-value: 1.88e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  10 LYGRLRQLAAADWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAILN-E 88
Cdd:cd19363    1 ITGKLRRDADDIWKKILNHPFVVELYSGTLPMEKFKFYLLQDYNYLVGSTKNLSILASKAESLDLMRELLELAYGEATtE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  89 VPLHVGYCAQWGISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGlglLHHpATRLEDNP---Y 165
Cdd:cd19363   81 FANYEELLDELGLSLEDAIKVEPFPTNVAYMNFLLSTSSLGSFYEGLAALLPCFWSYLEIA---EYH-KDKLSENPndiY 156

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 556283776 166 ASWIRNYGDEGYLQGVSAALALLETVWQQRGSEArlagLSDIFTTATRLEAQFWQM 221
Cdd:cd19363  157 RDWASVYLSKEYKELVERLRRIVDKYGEGEPFEK----LKRIFKTASKYEYMFWDA 208
TenA_E_Spr0628-like cd19358
TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C ...
 13-221 8.12e-27

TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C PET18; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E (not belonging to this family) hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP. Members of this family include the putative thiaminase Streptococcus pneumoniae Spr0628, and Saccharomyces cerevisiae S288C PET18, a protein of unknown function whose expression is induced in the absence of thiamin. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. Many proteins in this family have yet to be characterized.


Pssm-ID: 381693 [Multi-domain]  Cd Length: 209  Bit Score: 102.26  E-value: 8.12e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  13 RLRQLAAADWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAILNEvplH 92
Cdd:cd19358    4 RLRAANAEDWDAAVTHRFVRELCAGTLPDAVLARYLVQDYQFVETFLRLLGKAVAKAPDLEAKLRLARFLGFLAND---E 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  93 VGY---C-AQWGISESEMAAQPEAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGlglLHHPATRLEDNPYASW 168
Cdd:cd19358   81 NDYferAfAALGVSEADREAPPLLPATRAFIDLMLEAARSGSYAEILTVLLVAEWLYLDWA---SRAAAAAPLRFKHQEW 157

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|...
gi 556283776 169 IRNYGDEGYLQGVSAALALLETVWQQRGSEARlagLSDIFTTATRLEAQFWQM 221
Cdd:cd19358  158 IDLHSGPEFEAWVDFLRDEVDRVGPTEEERER---LEAVFARAVELEIAFFDA 207
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
13-227 3.43e-25

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 102.94  E-value: 3.43e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  13 RLRQLAAADWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLvSKLRTLPEMRA--AAASMNAILNEVP 90
Cdd:PRK14713 323 ALWQASGPIREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARL-AALAPDPAEQVfwAQSAQACLEVESE 401

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  91 LHVGYCA--QWGISESemaaqpeaPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGlLHHPATRLEDNPYASW 168
Cdd:PRK14713 402 LHRSWLGdrDADTAPS--------PVTLAYTDFLLARAAGGSYAVGAAAVLPCFWLYAEVGAE-LHARAGNPDDHPYAEW 472

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 556283776 169 IRNYGDEGYLQGVSAALALLETVWQQrGSEARLAGLSDIFTTATRLEAQFWQMGLNAAA 227
Cdd:PRK14713 473 LQTYADPEFAAATRRAIAFVDRAFRA-ASPAERAAMARAFLTACRYELEFFDQARRRAG 530
TenA_C_Bt3146-like cd19359
uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of ...
 22-219 6.28e-25

uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA-like proteins such as Bacteroides thetaiotaomicron Bt3146.


Pssm-ID: 381694 [Multi-domain]  Cd Length: 206  Bit Score: 97.42  E-value: 6.28e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  22 WQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMNAILNEVPLHVGYCAQW-G 100
Cdd:cd19359   12 WDKALNNPFCQGMADGTLDLDGFGYYMVQDYYYCINYVRFKALRAAKAPDPDLLAFLAAKIKSYLDYAEDFLKTCHIKlG 91

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776 101 ISESEMAAQPEAPETiNYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLgLLHHPATRLEDNPYASWIR-NYGDEGYLQ 179
Cdd:cd19359   92 IPDVVDGVKPSPALK-AYVDFERSVAESEDWFYLLVAMIPCIYLWYWLAN-QLNEDPSDKNTNFYKTWIEpNLPDPSSAK 169

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
gi 556283776 180 GVSAALALLETVWQQRGSEArlaglSDIFTTATRLEAQFW 219
Cdd:cd19359  170 QLEFFLNANAAWSKIDREKA-----NEIFRQAMQLEINFF 204
PTZ00347 PTZ00347
phosphomethylpyrimidine kinase; Provisional
 4-219 5.85e-20

phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 240375 [Multi-domain]  Cd Length: 504  Bit Score: 87.71  E-value: 5.85e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776   4 PAFSQGLYGRLRQLAAADWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLPEMRAAAASMN 83
Cdd:PTZ00347   7 EPVFGGLSEALWKENQDLAMMSLHLPFVQGLGDGTLDQNAFRTYIAQDTLYLNGYIRILSYCITKSDVTATGGGLLELLK 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  84 AILNEVP-LHVGYCaqwgisESEMAAQPEApETINYTRYVLDIGHSGD--ALDLLVALMPCVAGYAEIGLGLlhhPATRL 160
Cdd:PTZ00347  87 GVLEELKnCHHHYI------DNPDAAGPEA-ACRKYVDFLLASGNADTlgPSVVIAAVIPCARLYAWVGQEL---TNEVE 156

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 556283776 161 --EDNPYASWIRNYGDEGYLQGVSAALALLETvWQQRGSEARLAglsDIFTTATRLEAQFW 219
Cdd:PTZ00347 157 ltESHPFRRWLLSYSDEPINTSVEQLESLLDK-YIRPGEFSEVA---QAYRRAMELEYDFF 213
PRK09517 PRK09517
multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; ...
 29-214 9.27e-18

multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 169939 [Multi-domain]  Cd Length: 755  Bit Score: 81.56  E-value: 9.27e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  29 PFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLRTLP-EMRAAAASMNAILNEVPLHVGYcaqwgISESEMA 107
Cdd:PRK09517 565 DFIRMLGDGTLRRPEFDFYIDQDAQYLRQYSRALARLSSIAPDSHaQVEWAQSAAECIVVEAELHRSY-----LSGKEAP 639

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776 108 AQPeAPETINYTRYVLDIGHSGDALDLLVALMPCVAGYAEIGLGLlhhPATRLEDNPYASWIRNYGDEGYLQGVSAALAL 187
Cdd:PRK09517 640 SAP-SPVTMAYTDFLIARTYTEDYVVGVAAVLPCYWLYAEIGLML---AEQNHDEHPYKDWLNTYSGEEFIAGTRAAIAR 715

                        170       180       190
                 ....*....|....*....|....*....|....*..
gi 556283776 188 LETVWQQRGSEARLAGLS----------DIFTTATRL 214
Cdd:PRK09517 716 VEKALENAGPEQRVDAARaflsasvherEFFDQATRH 752
TenA_E_At3g16990-like cd19357
TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins ...
 14-221 1.34e-14

TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Members of this family include Arabidopsis thaliana At3g16990, Zea mays GRMZM2G080501, and Pyrococcus furiosus PF1337, among others. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin; nor does it have activity with other thiamine degradation products such as thiamine mono- or diphosphate, oxythiamine, oxothiamine, thiamine disulfide, desthiothiamine or thiochrome as substrates. Structural studies of P. furiosus PF1337 strongly support its enzymatic function in thiamine biosynthesis. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381692 [Multi-domain]  Cd Length: 217  Bit Score: 70.04  E-value: 1.34e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  14 LRQLAAADWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLR---TLPEMRAA---AASMNAILN 87
Cdd:cd19357    4 LLSLHPALYTAATQHPFLRAAADGTLPKEALSRWLAQDRLYVQAYIRFLGSLLARAPlpsSSLNQRLLdvlLGALANLRR 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  88 EVPLHVGYCAQWGISESEMAAQPeAPETINYTRYVLDIGHSG-DALDLLVALmpcvagYA-------------EIGLGLL 153
Cdd:cd19357   84 ELAFFEETAAEYGLDLPGLGVPP-SPATRAYVDFLASLASEGvSYLEGLVVL------WAtekvyldawsyarSFLPSDA 156

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 556283776 154 HHPAtrlednPYASWIRNYGDEGYLQGVSAALALLETVWQQRGSEARlAGLSDIFTTATRLEAQFWQM 221
Cdd:cd19357  157 DGGA------LYREFIPNWTSPEFAAFVDRLGDLVDEALEQAGEEVL-ERAEEVWRRVLELEEAFWPM 217
TenA_C_SsTenA-1-like cd19362
uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This ...
 13-223 3.44e-14

uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA like proteins such as Sulfolobus solfataricus putative TenA-like thiaminase (Tena-1, Sso2206).


Pssm-ID: 381697 [Multi-domain]  Cd Length: 200  Bit Score: 68.62  E-value: 3.44e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  13 RLRQLAAADWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSyallVSKLRTLPEMRAAAASMNAILN----- 87
Cdd:cd19362    3 NLKNLVGDLWNKYVRHEFVERMRDGTLPLDNFRYYLIQDSKYVEEMLRA----LLRASSKAPLDKAIKILNSVFSgrdkg 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776  88 -EVplHVGYCAQWGISESEMAAQPEAPETINYTR---YVLDIGHSgdalDLLVALMPCVAGYAEIGLGLLHHPatrleDN 163
Cdd:cd19362   79 mEV--HKFLYSELGITEDEIRRTGYNLVNYAYTRhlyYYSTLGWP----QFLAAWAPCMWGYSEIGKYVLNSP-----NE 147

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 556283776 164 PYASWIRNYGDEGYLQGVSAALALLETVwqqrgseARLAGLSDIFTTATRLEAQFWQMGL 223
Cdd:cd19362  148 LYKTWASFYASKDYKKRVEAILEALDSI-------EDTEDIKNIFRNSVNFEIMFWDAAL 200
PqqC COG5424
Pyrroloquinoline quinone (PQQ) biosynthesis protein C [Coenzyme transport and metabolism];
10-78 5.08e-05

Pyrroloquinoline quinone (PQQ) biosynthesis protein C [Coenzyme transport and metabolism];


Pssm-ID: 444176  Cd Length: 228  Bit Score: 42.96  E-value: 5.08e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 556283776  10 LYGRLRQLAAadWQHYVAHPFVQQLAEGTLAKSAFRRYLTQDYLFLIHFARSYALLVSKLrTLPEMRAA 78
Cdd:COG5424    7 FEARLRAEIA--RRYLLKHPFLQRLREGKLTREQLRAFALQRYHYVKHFPRYLAAILSRC-PDEELRRA 72
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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