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Conserved domains on  [gi|556315167|ref|WP_023294805|]
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MULTISPECIES: putative lipopolysaccharide heptosyltransferase III [Enterobacter]

Protein Classification

glycosyltransferase family 9 protein( domain architecture ID 11436709)

glycosyltransferase family 9 protein may functions as a lipopolysaccharide heptosyltransferase involved in the biosynthesis of lipooligosaccharide (LOS)

CAZY:  GT9
EC:  2.4.-.-
Gene Ontology:  GO:0016757
PubMed:  12691742|16037492
SCOP:  3001586

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
RfaF COG0859
ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis];
9-362 1.07e-106

ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis];


:

Pssm-ID: 440620  Cd Length: 336  Bit Score: 316.53  E-value: 1.07e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   9 RPPKRVLIIKLRHHGDVLLTSPVFTVLKKNWPNVEVDALVYDDTQAMLTSHPHIDQVHTIgrnwrKKGWFEQFRLYRALL 88
Cdd:COG0859    2 QPPMRILIIRLSALGDVLLATPALRALKRAYPDAEIDLLVEPRFAPLLELNPYVDEVIPF-----DKKRRKGLAELLKLL 76
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  89 NTLKGRQYDVLINLTEHWHGARLARRLKPRVSVGFKPDKRGGLarrrwvksFTTLYPAIQDnsRHMVEVNLDALRRIGIH 168
Cdd:COG0859   77 RQLRAERYDLVIDLQGSLRSALLARLAGAPRRIGFDKELRSLL--------LNHRVPLPPD--QHEVERYLALLAALGIP 146
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 169 PqsDEDKHTLFVPgDAAEASVAEKLAGFGLTSKSYILVHPTSRWMFKAWDIKKLAATIDNLAARGLPIVLSAAPskEEKA 248
Cdd:COG0859  147 L--PDPRPDLPLP-EEDRAEARALLARLGLPGKPYIVLHPGASWPAKRWPAERFAELARALAARGLRVVLLGGP--GERE 221
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 249 YMDALRAALTQPVFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPTGAIKWAPWGVNYRVIVAGFT 328
Cdd:COG0859  222 LAEEIAAALGPPVINLAGKTSLRELAALLARADLVVGNDTGPMHLAAALGTPTVALFGPTDPARWGPYGDRHRVLRADLP 301
                        330       340       350
                 ....*....|....*....|....*....|....
gi 556315167 329 CQPCGKAGCgDSGVSDCITAITPQQVLSAIDTML 362
Cdd:COG0859  302 CSPCGKREC-PLGHHPCMADISPEEVLEALEELL 334
 
Name Accession Description Interval E-value
RfaF COG0859
ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis];
9-362 1.07e-106

ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440620  Cd Length: 336  Bit Score: 316.53  E-value: 1.07e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   9 RPPKRVLIIKLRHHGDVLLTSPVFTVLKKNWPNVEVDALVYDDTQAMLTSHPHIDQVHTIgrnwrKKGWFEQFRLYRALL 88
Cdd:COG0859    2 QPPMRILIIRLSALGDVLLATPALRALKRAYPDAEIDLLVEPRFAPLLELNPYVDEVIPF-----DKKRRKGLAELLKLL 76
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  89 NTLKGRQYDVLINLTEHWHGARLARRLKPRVSVGFKPDKRGGLarrrwvksFTTLYPAIQDnsRHMVEVNLDALRRIGIH 168
Cdd:COG0859   77 RQLRAERYDLVIDLQGSLRSALLARLAGAPRRIGFDKELRSLL--------LNHRVPLPPD--QHEVERYLALLAALGIP 146
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 169 PqsDEDKHTLFVPgDAAEASVAEKLAGFGLTSKSYILVHPTSRWMFKAWDIKKLAATIDNLAARGLPIVLSAAPskEEKA 248
Cdd:COG0859  147 L--PDPRPDLPLP-EEDRAEARALLARLGLPGKPYIVLHPGASWPAKRWPAERFAELARALAARGLRVVLLGGP--GERE 221
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 249 YMDALRAALTQPVFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPTGAIKWAPWGVNYRVIVAGFT 328
Cdd:COG0859  222 LAEEIAAALGPPVINLAGKTSLRELAALLARADLVVGNDTGPMHLAAALGTPTVALFGPTDPARWGPYGDRHRVLRADLP 301
                        330       340       350
                 ....*....|....*....|....*....|....
gi 556315167 329 CQPCGKAGCgDSGVSDCITAITPQQVLSAIDTML 362
Cdd:COG0859  302 CSPCGKREC-PLGHHPCMADISPEEVLEALEELL 334
heptsyl_trn_III TIGR02201
lipopolysaccharide heptosyltransferase III, putative; This family consists of examples of the ...
13-326 5.12e-93

lipopolysaccharide heptosyltransferase III, putative; This family consists of examples of the putative ADP-heptose:LPS heptosyltransferase III, an enzyme of LPS inner core region biosynthesis. LPS, composed of lipid A, a core region, and O antigen, is found in the outer membrane of Gram-negative bacteria. This enzyme may be less widely distributed than heptosyltransferases I and II. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]


Pssm-ID: 131256  Cd Length: 344  Bit Score: 281.79  E-value: 5.12e-93
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   13 RVLIIKLRHHGDVLLTSPVFTVLKKNWPNVEVDALVYDDTQAMLTSHPHIDQVHTIGRNWRKKGWfEQFRLYRALLNTLK 92
Cdd:TIGR02201   1 RILLIKLRHHGDMLLTTPVISSLKKNYPDAKIDVLLYQETIPILSENPDINALYGLDRKKAKAGE-RKLANQFHLIKVLR 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   93 GRQYDVLINLTEHWHGARLARRLKPRVSVGFKPDKRgglARRRWVKSFTTLYPAIQDNSRHMVEVNLDALRRIGIHPQSD 172
Cdd:TIGR02201  80 ANRYDLVVNLTDQWMVAILVKLLNARVKIGFDYPKR---RSAFWRKSFTALAPLQGGNTLHTVEQNLSVLTPLGLDSLVK 156
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  173 EdkHTLFVPGDAAEASVAE-KLAGfglTSKSYILVHPTSRWMFKAWDIKKLAATIDNLAARGLPIVLSAAPSKEEKAYMD 251
Cdd:TIGR02201 157 Q--TRMSYPPADWKAMRALlDEAG---VGQNYIVIQPTSRWFFKCWDNDRFSALIDALHARGYEVVLTSGPDKDELAMVN 231
                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 556315167  252 ALRAALTQP-VFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPTGAIKWAPWGVNYRVIVAG 326
Cdd:TIGR02201 232 EIAQGCQTPrVTSLAGKLTLPQLAALIDHARLFIGVDSVPMHMAAALGTPLVALFGPSKHIFWRPWSNNMIQFWAG 307
PRK10422 PRK10422
lipopolysaccharide core biosynthesis protein; Provisional
8-369 1.01e-81

lipopolysaccharide core biosynthesis protein; Provisional


Pssm-ID: 182447  Cd Length: 352  Bit Score: 253.16  E-value: 1.01e-81
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   8 ERPPKRVLIIKLRHHGDVLLTSPVFTVLKKNWPNVEVDALVYDDTQAMLTSHPHIDQVHTIGRnwRKKGWFEQFRLYRAL 87
Cdd:PRK10422   2 DKPFRRILIIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQDTIPILSENPEINALYGIKN--KKAGASEKIKNFFSL 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  88 LNTLKGRQYDVLINLTEHWHGARLARRLKPRVSVGFKPDKRGGlarRRWVKSFTTLYPAIQDnsrHMVEVNLDALRRIGI 167
Cdd:PRK10422  80 IKVLRANKYDLIVNLTDQWMVALLVRLLNARVKISQDYHHRQS---AFWRKSFTHLVPLQGG---HIVESNLSVLTPLGL 153
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 168 hpqSDEDKHTLFVPGDAAEASVAEKLAGFGLTSKsYILVHPTSRWMFKAWDIKKLAATIDNLAARGLPIVLSAAPSKEEK 247
Cdd:PRK10422 154 ---SSLVKETTMSYRPESWKRMRRQLDHLGVTQN-YVVIQPTARQIFKCWDNDKFSAVIDALQARGYEVVLTSGPDKDDL 229
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 248 AYMDAL-RAALTQPVFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPTGAIKWAPWGVNYRVIVAG 326
Cdd:PRK10422 230 ACVNEIaQGCQTPPVTALAGKTTFPELGALIDHAQLFIGVDSAPAHIAAAVNTPLICLFGATDHIFWRPWSDNMIQFWAG 309
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|...
gi 556315167 327 fTCQPCGKAGCGDSGVSdCITAITPQQVLSAIDTMLLETETPA 369
Cdd:PRK10422 310 -DYQEMPTRDELDRNEK-YLSVIPAADVIAAVDKLLPSSTTGT 350
GT9_LPS_heptosyltransferase cd03789
lipopolysaccharide heptosyltransferase and similar proteins; Lipopolysaccharide ...
13-359 6.74e-73

lipopolysaccharide heptosyltransferase and similar proteins; Lipopolysaccharide heptosyltransferase (2.4.99.B6) is involved in the biosynthesis of lipooligosaccharide (LOS). Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria. LPS heptosyltransferase transfers heptose molecules from ADP-heptose to 3-deoxy-D-manno-octulosonic acid (KDO), a part of the inner core component of LPS. This family also contains lipopolysaccharide 1,2-N-acetylglucosaminetransferase EC 2.4.1.56 and belongs to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.


Pssm-ID: 340821  Cd Length: 277  Bit Score: 228.00  E-value: 6.74e-73
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  13 RVLIIKLRHHGDVLLTSPVFTVLKKNWPNVEVDALVYDDTQAMLTSHPHIDQVHTIgrnwrKKGWFEQFRLYRALLNTLK 92
Cdd:cd03789    1 KILVIRLSALGDVVLTTPLLRALKKAYPDAKITVVVGPGYAELLELNPYIDEVIPD-----DKRRRAGLRGRRKLLRELR 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  93 GRQYDVLINLTEHWHGARLARRLKPRVSVGFKPDKRGGLARRRWVKsfttlypaiqdnsrhmvevnldalrrigihpqsd 172
Cdd:cd03789   76 ARKYDLVIDLHSSLRSALLLLLSGAPRRIGFDREKRRFLLNHPVKP---------------------------------- 121
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 173 edkhtlfvpgdaaeasvaeklagfgltsksYILVHPTSRWMFKAWDIKKLAATIDNLAARGLPIVLSAAPSKEEKAymDA 252
Cdd:cd03789  122 ------------------------------LVVIPPGASGPAKRWPAERFAELADRLADEGYRVVLFGGPAEEELA--EE 169
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 253 LRAALTQPVFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPTGAIKWAPWGVNYRVIVAGFTCQPC 332
Cdd:cd03789  170 IAAALGARVVNLAGKTSLRELAALLARADLVVGNDSGPMHLAAALGTPTVALFGPTDPARTGPYGSNHVVVRADLPCSPC 249
                        330       340
                 ....*....|....*....|....*..
gi 556315167 333 GKAGCGDSGVSDCITAITPQQVLSAID 359
Cdd:cd03789  250 CPKRECPRGDHKCMRDITPEEVIEAIR 276
Glyco_transf_9 pfam01075
Glycosyltransferase family 9 (heptosyltransferase); Members of this family belong to ...
85-337 2.04e-40

Glycosyltransferase family 9 (heptosyltransferase); Members of this family belong to glycosyltransferase family 9. Lipopolysaccharide is a major component of the outer leaflet of the outer membrane in Gram-negative bacteria. It is composed of three domains; lipid A, Core oligosaccharide and the O-antigen. All of these enzymes transfer heptose to the lipopolysaccharide core.


Pssm-ID: 395853  Cd Length: 247  Bit Score: 142.85  E-value: 2.04e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   85 RALLNTLKGRQYDVLINLTEHWHGARLARRLKPRVSVGFkpdkRGGLARRRWVKSFTTLYPAIQdnsRHMVEVNLDALrr 164
Cdd:pfam01075   2 RRLGKALRANAYDRVIDLQGLLKSALLVRFLKAPPRIGF----DGESRRLGSLFYSRKHDKPKG---PHAVERNRALF-- 72
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  165 igiHPQSDEDKHTLF--VPGDAAEASVAEKLAGFGltsKSYILVHPTSRWMFKAWDIKKLAATIDNLAARGLPIVLSAAP 242
Cdd:pfam01075  73 ---AQALGLPKPESKpeLGLSLPFRAAALDAAGAG---RPYIVFCPGASFDAKRWPEEHYAELAEALQERGYQVVLFGGP 146
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  243 SKEEKAYMDALRAALTQPVFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPTGAIKWAPWGVNYRV 322
Cdd:pfam01075 147 EAHEEEIAERIAAGLEETCVNLLGKTSLEEAAALLAGADLVVGNDSGLMHLAAALDRPVIGLYGPTDPGRTPPYSDNATI 226
                         250
                  ....*....|....*
gi 556315167  323 IVAGFTCQPCGKAGC 337
Cdd:pfam01075 227 VSLHEGCSPCFKKTC 241
 
Name Accession Description Interval E-value
RfaF COG0859
ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis];
9-362 1.07e-106

ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440620  Cd Length: 336  Bit Score: 316.53  E-value: 1.07e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   9 RPPKRVLIIKLRHHGDVLLTSPVFTVLKKNWPNVEVDALVYDDTQAMLTSHPHIDQVHTIgrnwrKKGWFEQFRLYRALL 88
Cdd:COG0859    2 QPPMRILIIRLSALGDVLLATPALRALKRAYPDAEIDLLVEPRFAPLLELNPYVDEVIPF-----DKKRRKGLAELLKLL 76
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  89 NTLKGRQYDVLINLTEHWHGARLARRLKPRVSVGFKPDKRGGLarrrwvksFTTLYPAIQDnsRHMVEVNLDALRRIGIH 168
Cdd:COG0859   77 RQLRAERYDLVIDLQGSLRSALLARLAGAPRRIGFDKELRSLL--------LNHRVPLPPD--QHEVERYLALLAALGIP 146
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 169 PqsDEDKHTLFVPgDAAEASVAEKLAGFGLTSKSYILVHPTSRWMFKAWDIKKLAATIDNLAARGLPIVLSAAPskEEKA 248
Cdd:COG0859  147 L--PDPRPDLPLP-EEDRAEARALLARLGLPGKPYIVLHPGASWPAKRWPAERFAELARALAARGLRVVLLGGP--GERE 221
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 249 YMDALRAALTQPVFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPTGAIKWAPWGVNYRVIVAGFT 328
Cdd:COG0859  222 LAEEIAAALGPPVINLAGKTSLRELAALLARADLVVGNDTGPMHLAAALGTPTVALFGPTDPARWGPYGDRHRVLRADLP 301
                        330       340       350
                 ....*....|....*....|....*....|....
gi 556315167 329 CQPCGKAGCgDSGVSDCITAITPQQVLSAIDTML 362
Cdd:COG0859  302 CSPCGKREC-PLGHHPCMADISPEEVLEALEELL 334
heptsyl_trn_III TIGR02201
lipopolysaccharide heptosyltransferase III, putative; This family consists of examples of the ...
13-326 5.12e-93

lipopolysaccharide heptosyltransferase III, putative; This family consists of examples of the putative ADP-heptose:LPS heptosyltransferase III, an enzyme of LPS inner core region biosynthesis. LPS, composed of lipid A, a core region, and O antigen, is found in the outer membrane of Gram-negative bacteria. This enzyme may be less widely distributed than heptosyltransferases I and II. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]


Pssm-ID: 131256  Cd Length: 344  Bit Score: 281.79  E-value: 5.12e-93
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   13 RVLIIKLRHHGDVLLTSPVFTVLKKNWPNVEVDALVYDDTQAMLTSHPHIDQVHTIGRNWRKKGWfEQFRLYRALLNTLK 92
Cdd:TIGR02201   1 RILLIKLRHHGDMLLTTPVISSLKKNYPDAKIDVLLYQETIPILSENPDINALYGLDRKKAKAGE-RKLANQFHLIKVLR 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   93 GRQYDVLINLTEHWHGARLARRLKPRVSVGFKPDKRgglARRRWVKSFTTLYPAIQDNSRHMVEVNLDALRRIGIHPQSD 172
Cdd:TIGR02201  80 ANRYDLVVNLTDQWMVAILVKLLNARVKIGFDYPKR---RSAFWRKSFTALAPLQGGNTLHTVEQNLSVLTPLGLDSLVK 156
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  173 EdkHTLFVPGDAAEASVAE-KLAGfglTSKSYILVHPTSRWMFKAWDIKKLAATIDNLAARGLPIVLSAAPSKEEKAYMD 251
Cdd:TIGR02201 157 Q--TRMSYPPADWKAMRALlDEAG---VGQNYIVIQPTSRWFFKCWDNDRFSALIDALHARGYEVVLTSGPDKDELAMVN 231
                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 556315167  252 ALRAALTQP-VFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPTGAIKWAPWGVNYRVIVAG 326
Cdd:TIGR02201 232 EIAQGCQTPrVTSLAGKLTLPQLAALIDHARLFIGVDSVPMHMAAALGTPLVALFGPSKHIFWRPWSNNMIQFWAG 307
PRK10422 PRK10422
lipopolysaccharide core biosynthesis protein; Provisional
8-369 1.01e-81

lipopolysaccharide core biosynthesis protein; Provisional


Pssm-ID: 182447  Cd Length: 352  Bit Score: 253.16  E-value: 1.01e-81
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   8 ERPPKRVLIIKLRHHGDVLLTSPVFTVLKKNWPNVEVDALVYDDTQAMLTSHPHIDQVHTIGRnwRKKGWFEQFRLYRAL 87
Cdd:PRK10422   2 DKPFRRILIIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQDTIPILSENPEINALYGIKN--KKAGASEKIKNFFSL 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  88 LNTLKGRQYDVLINLTEHWHGARLARRLKPRVSVGFKPDKRGGlarRRWVKSFTTLYPAIQDnsrHMVEVNLDALRRIGI 167
Cdd:PRK10422  80 IKVLRANKYDLIVNLTDQWMVALLVRLLNARVKISQDYHHRQS---AFWRKSFTHLVPLQGG---HIVESNLSVLTPLGL 153
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 168 hpqSDEDKHTLFVPGDAAEASVAEKLAGFGLTSKsYILVHPTSRWMFKAWDIKKLAATIDNLAARGLPIVLSAAPSKEEK 247
Cdd:PRK10422 154 ---SSLVKETTMSYRPESWKRMRRQLDHLGVTQN-YVVIQPTARQIFKCWDNDKFSAVIDALQARGYEVVLTSGPDKDDL 229
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 248 AYMDAL-RAALTQPVFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPTGAIKWAPWGVNYRVIVAG 326
Cdd:PRK10422 230 ACVNEIaQGCQTPPVTALAGKTTFPELGALIDHAQLFIGVDSAPAHIAAAVNTPLICLFGATDHIFWRPWSDNMIQFWAG 309
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|...
gi 556315167 327 fTCQPCGKAGCGDSGVSdCITAITPQQVLSAIDTMLLETETPA 369
Cdd:PRK10422 310 -DYQEMPTRDELDRNEK-YLSVIPAADVIAAVDKLLPSSTTGT 350
GT9_LPS_heptosyltransferase cd03789
lipopolysaccharide heptosyltransferase and similar proteins; Lipopolysaccharide ...
13-359 6.74e-73

lipopolysaccharide heptosyltransferase and similar proteins; Lipopolysaccharide heptosyltransferase (2.4.99.B6) is involved in the biosynthesis of lipooligosaccharide (LOS). Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria. LPS heptosyltransferase transfers heptose molecules from ADP-heptose to 3-deoxy-D-manno-octulosonic acid (KDO), a part of the inner core component of LPS. This family also contains lipopolysaccharide 1,2-N-acetylglucosaminetransferase EC 2.4.1.56 and belongs to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.


Pssm-ID: 340821  Cd Length: 277  Bit Score: 228.00  E-value: 6.74e-73
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  13 RVLIIKLRHHGDVLLTSPVFTVLKKNWPNVEVDALVYDDTQAMLTSHPHIDQVHTIgrnwrKKGWFEQFRLYRALLNTLK 92
Cdd:cd03789    1 KILVIRLSALGDVVLTTPLLRALKKAYPDAKITVVVGPGYAELLELNPYIDEVIPD-----DKRRRAGLRGRRKLLRELR 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  93 GRQYDVLINLTEHWHGARLARRLKPRVSVGFKPDKRGGLARRRWVKsfttlypaiqdnsrhmvevnldalrrigihpqsd 172
Cdd:cd03789   76 ARKYDLVIDLHSSLRSALLLLLSGAPRRIGFDREKRRFLLNHPVKP---------------------------------- 121
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 173 edkhtlfvpgdaaeasvaeklagfgltsksYILVHPTSRWMFKAWDIKKLAATIDNLAARGLPIVLSAAPSKEEKAymDA 252
Cdd:cd03789  122 ------------------------------LVVIPPGASGPAKRWPAERFAELADRLADEGYRVVLFGGPAEEELA--EE 169
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 253 LRAALTQPVFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPTGAIKWAPWGVNYRVIVAGFTCQPC 332
Cdd:cd03789  170 IAAALGARVVNLAGKTSLRELAALLARADLVVGNDSGPMHLAAALGTPTVALFGPTDPARTGPYGSNHVVVRADLPCSPC 249
                        330       340
                 ....*....|....*....|....*..
gi 556315167 333 GKAGCGDSGVSDCITAITPQQVLSAID 359
Cdd:cd03789  250 CPKRECPRGDHKCMRDITPEEVIEAIR 276
Glyco_transf_9 pfam01075
Glycosyltransferase family 9 (heptosyltransferase); Members of this family belong to ...
85-337 2.04e-40

Glycosyltransferase family 9 (heptosyltransferase); Members of this family belong to glycosyltransferase family 9. Lipopolysaccharide is a major component of the outer leaflet of the outer membrane in Gram-negative bacteria. It is composed of three domains; lipid A, Core oligosaccharide and the O-antigen. All of these enzymes transfer heptose to the lipopolysaccharide core.


Pssm-ID: 395853  Cd Length: 247  Bit Score: 142.85  E-value: 2.04e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   85 RALLNTLKGRQYDVLINLTEHWHGARLARRLKPRVSVGFkpdkRGGLARRRWVKSFTTLYPAIQdnsRHMVEVNLDALrr 164
Cdd:pfam01075   2 RRLGKALRANAYDRVIDLQGLLKSALLVRFLKAPPRIGF----DGESRRLGSLFYSRKHDKPKG---PHAVERNRALF-- 72
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  165 igiHPQSDEDKHTLF--VPGDAAEASVAEKLAGFGltsKSYILVHPTSRWMFKAWDIKKLAATIDNLAARGLPIVLSAAP 242
Cdd:pfam01075  73 ---AQALGLPKPESKpeLGLSLPFRAAALDAAGAG---RPYIVFCPGASFDAKRWPEEHYAELAEALQERGYQVVLFGGP 146
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  243 SKEEKAYMDALRAALTQPVFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPTGAIKWAPWGVNYRV 322
Cdd:pfam01075 147 EAHEEEIAERIAAGLEETCVNLLGKTSLEEAAALLAGADLVVGNDSGLMHLAAALDRPVIGLYGPTDPGRTPPYSDNATI 226
                         250
                  ....*....|....*
gi 556315167  323 IVAGFTCQPCGKAGC 337
Cdd:pfam01075 227 VSLHEGCSPCFKKTC 241
heptsyl_trn_I TIGR02193
lipopolysaccharide heptosyltransferase I; This family consists of examples of ADP-heptose:LPS ...
13-362 2.51e-31

lipopolysaccharide heptosyltransferase I; This family consists of examples of ADP-heptose:LPS heptosyltransferase I, an enzyme of LPS inner core region biosynthesis. LPS, composed of lipid A, a core region, and O antigen, is found in the outer membrane of Gram-negative bacteria. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]


Pssm-ID: 274025  Cd Length: 319  Bit Score: 120.52  E-value: 2.51e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   13 RVLIIKLRHHGDVLLTSPVFTVLKKNWPNVEVDALVYDDTQAMLTSHPHIDQVHTIG-RNWRKKGWF-EQFRLYRALLNT 90
Cdd:TIGR02193   1 RILIVKTSSLGDVIHTLPALTDIKRALPDVEIDWVVEEGFADIVRLHPAVDEVIPVAlRRWRKTLFSaATWREIKALRAL 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   91 LKGRQYDVLINLTEHWHGARLARRLK-PRVSVGFKPDkRGGLARRrwvksfttLYpaiqdNSRHMVEVNLDALRRI---- 165
Cdd:TIGR02193  81 LRAERYDAVIDAQGLIKSALVARMARgPRHGFDWRSA-REPLASL--------FY-----NKRVGISYQQHAVERNrklf 146
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  166 ----GIHPQSDEdkhtlfvPGDAAEASVAEK-LAGFGLTSKSYILVHPTSRwMFKAWDIKKLAATIDNLAARGLPIVLSA 240
Cdd:TIGR02193 147 alalGYPPPIAE-------TIDYGLARRAAVaFLGHALPAPYAVLLHATSR-DDKTWPEERWRELARLLLARGLQIVLPW 218
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  241 ApSKEEKAYMDALRAALtqPVFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPTGAIKWAPWGVNY 320
Cdd:TIGR02193 219 G-NDAEKQRAERIAEAL--PGAVVLPKMSLAEVAALLAGADAVVGVDTGLTHLAAALDKPTVTLYGATDPGRTGGYGKPN 295
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|..
gi 556315167  321 RVIVagftcqpcgkagcGDSGVSdcitaITPQQVLSAIDTML 362
Cdd:TIGR02193 296 VALL-------------GESGAN-----PTPDEVLAALEELL 319
heptsyl_trn_II TIGR02195
lipopolysaccharide heptosyltransferase II; This family consists of examples of ADP-heptose:LPS ...
13-363 1.53e-20

lipopolysaccharide heptosyltransferase II; This family consists of examples of ADP-heptose:LPS heptosyltransferase II, an enzyme of LPS inner core region biosynthesis. LPS, composed of lipid A, a core region, and O antigen, is found in the outer membrane of Gram-negative bacteria. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]


Pssm-ID: 274026  Cd Length: 334  Bit Score: 91.28  E-value: 1.53e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   13 RVLIIKLRHHGDVLLTSPVFTVLKKNWPNVEVDALVYDDTQAMLTSHPHIDQVHTigrNWRKKGWFEqFRLYRALLNTLK 92
Cdd:TIGR02195   1 KILVIGPSWVGDMVMAQSLYRLLKKRYPQAVIDVLAPAWCRPLLERMPEIRQAID---MPLGHGALE-LTERRRLGRSLR 76
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167   93 GRQYDVLINLTEHWHGARLARRLKPRVSVGFKPDKRGGLA--RRRWVKSFttlYPAIQDNSRHMVEVNLDALRRIGIHPQ 170
Cdd:TIGR02195  77 EERYDQAIVLPNSLKSALIPFFAGIPHRTGWRGEMRYGLLndVRALDKER---LPLMVERYIALAYDKGQDLPQPLPRPQ 153
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  171 SDEDKhtlfvpgdaaeASVAEKLAGFGL-TSKSYILVHPTSRW-MFKAWDIKKLAATIDNLAARGLPIVLSAapSKEEKA 248
Cdd:TIGR02195 154 LQVSP-----------AEQAAALAKFGLdTERPIIAFCPGAEFgPAKRWPHEHYAELAKRLIDQGYQVVLFG--SAKDHP 220
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  249 YMDALRAALTQPVFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPTGAIKWAPWGVNYRVIVAGFT 328
Cdd:TIGR02195 221 AGNEIEALLPGELRNLAGETSLDEAVDLIALAKAVVTNDSGLMHVAAALNRPLVALYGSTSPDFTPPLSEKAEVIRLNLE 300
                         330       340       350
                  ....*....|....*....|....*....|....*
gi 556315167  329 CQPCGKAGCgDSGVSDCITAITPQQVLSAIDTMLL 363
Cdd:TIGR02195 301 CSPCFKRDC-PYGHHQCLIDLSPEQVLEALNELLL 334
WcaK COG2327
Polysaccharide pyruvyl transferase family protein WcaK (colanic acid biosynthesis) [Cell wall ...
23-362 2.35e-10

Polysaccharide pyruvyl transferase family protein WcaK (colanic acid biosynthesis) [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441900 [Multi-domain]  Cd Length: 357  Bit Score: 61.22  E-value: 2.35e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  23 GDVLLTSPVFTVLKKNWPNVEVDALVYDDTQamlTSHPHidQVHTIGRNWRkkgwfeqFRLYRALlntlkgRQYDVLI-- 100
Cdd:COG2327   15 GDEALLEALLQLLRKRLPDAEITVLSGNPEE---TRKRY--GVRAVPRRNF-------LEILRAL------RRADLVIsg 76
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 101 ------------NLTEHWHGARLARRLKPRV---SVGFKPDKRGgLARRRWVKSFttlypaiqDNSRHMV---EVNLDAL 162
Cdd:COG2327   77 gggllqdvtslrSLLYYLGLVLLAKLLGKPVviyGQGIGPFKSP-LSRRLLRRVL--------NRADLISvrdEASAELL 147
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 163 RRIGIHPQSDEdkhtlfVPGDAAEASVAEKLAGFGLTSKSYILVHPTSRWMFKAWDIKKLAATIDNLAAR-GLPIVLSA- 240
Cdd:COG2327  148 KDLGVKKPPIE------LTPDPAFLLPPEPVDKEAPLERPVVGVNLRPWRGLEEDYLEALAAALDRLIERtGYKVVLLPh 221
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 241 -APSKEEKAYMDALRAALTQPVFDLSGQLNLKELGALMKHARVYFGvdsMPMH---LASAVGTPTVAIfgptgaikwapw 316
Cdd:COG2327  222 qYGQEDDLAIAEELAAKLKERVVVLDEELDPDELLSLIARADLVIG---MRLHaliFALSAGVPVIAL------------ 286
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....*.
gi 556315167 317 gvNYRVIVAGFtcqpCGKAGCGDSGVSdcITAITPQQVLSAIDTML 362
Cdd:COG2327  287 --SYDPKVRGL----MEDLGLPDYVLD--LDDLDPEELLAAVEELL 324
PRK10964 PRK10964
lipopolysaccharide heptosyltransferase RfaC;
13-308 1.54e-09

lipopolysaccharide heptosyltransferase RfaC;


Pssm-ID: 236809  Cd Length: 322  Bit Score: 58.45  E-value: 1.54e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  13 RVLIIKLRHHGDVLLTSPVFTVLKKNWPNVEVDALVYDDTQAMLTSHPHIDQVHTIG-RNWRKKgWFE-QFRLYRALLNT 90
Cdd:PRK10964   2 RVLIVKTSSMGDVLHTLPALTDAQQAIPGIQFDWVVEEGFAQIPSWHPAVDRVIPVAiRRWRKA-WFSaPIRAERKAFRE 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167  91 -LKGRQYDVLINLTEHWHGARLARRLKPRVSVGFkpDK---RGGLArrrwvkSFttLYpaiqdNSRHMVEVNLDALRRIg 166
Cdd:PRK10964  81 aLQAEQYDAVIDAQGLVKSAALVTRLAHGVKHGM--DWqsaREPLA------SL--FY-----NRRHHIAKQQHAVERT- 144
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 167 ihpqsdedkHTLFV----------PGDAAEAS--VAEKLAGFGltskSYIL-VHPTSR----WMFKAWdiKKLaatIDNL 229
Cdd:PRK10964 145 ---------RELFAkslgyskpqtQGDYAIAQhfLTNLPADAG----PYLVfLHATTRddkhWPEAHW--REL---IGLL 206
                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 556315167 230 AARGLPIVLsaaPSKEEKAYMDALRAALTQPVFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPT 308
Cdd:PRK10964 207 APSGLRIKL---PWGAEHEEQRAKRLAEGFPYVEVLPKLSLEQVARVLAGAKAVVSVDTGLSHLTAALDRPNITLYGPT 282
PRK10916 PRK10916
ADP-heptose--LPS heptosyltransferase RfaF;
186-366 4.16e-07

ADP-heptose--LPS heptosyltransferase RfaF;


Pssm-ID: 182835  Cd Length: 348  Bit Score: 51.21  E-value: 4.16e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 186 EASVAEKLAGFGLTSKSYIL-------VHPTSRWMFKAWdikklAATIDNLAARGLPIVLSAapSKEEKAYMDALRAALT 258
Cdd:PRK10916 164 EGEKSETCAAFSLSSERPIIgfcpgaeFGPAKRWPHYHY-----AELAQQLIDEGYQVVLFG--SAKDHEAGNEILAALN 236
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556315167 259 ----QPVFDLSGQLNLKELGALMKHARVYFGVDSMPMHLASAVGTPTVAIFGPTGAIKWAPWGVNYRVI--VAGFTcqpc 332
Cdd:PRK10916 237 teqqAWCRNLAGETQLEQAVILIAACKAIVTNDSGLMHVAAALNRPLVALYGPSSPDFTPPLSHKARVIrlITGYH---- 312
                        170       180       190
                 ....*....|....*....|....*....|....*.
gi 556315167 333 gKAGCGDS--GVSDCITAITPQQVLSAIDTMLLETE 366
Cdd:PRK10916 313 -KVRKGDAaeGYHQSLIDITPQRVLEELNALLLQEE 347
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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