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Conserved domains on  [gi|556464597|ref|WP_023316379|]
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MULTISPECIES: type IV secretion system protein [Enterobacterales]

Protein Classification

type IV secretion system protein( domain architecture ID 10547094)

type IV secretion system protein similar to Brucella abortus type IV secretion system protein virB5

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
T4SS pfam07996
Type IV secretion system proteins; Members of this family are components of the type IV ...
 22-216 6.10e-37

Type IV secretion system proteins; Members of this family are components of the type IV secretion system. They mediate intracellular transfer of macromolecules via a mechanism ancestrally related to that of bacterial conjugation machineries.


:

Pssm-ID: 429777 [Multi-domain]  Cd Length: 189  Bit Score: 128.23  E-value: 6.10e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556464597   22 GIPVaVDADPMrdVQWAQELKRWMETARHYQSQIQAYKDQLATATGVRDIADFVDQAKGLKADLEKLRKPGQALNDLLLS 101
Cdd:pfam07996   1 GIPV-IDAAAI--AQAIAQVVEAIAQLTQLKQQINQYKQQYNSLTGARGLGDILNNPALRNYLPADWQDIYDLVKSGGSY 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556464597  102 GGSSGQFDALYEKYKIFDTCNtaqSGSYANVCKQQVINKAIQLEQTDEVQNQVSQTLGEINSLSNRVALAKDSKESQDLA 181
Cdd:pfam07996  78 GSLSSAAQSLRDANKLYDVCT---DDDRADACQQAANKAAQDKAFAEQAYDTATQRLDQIQQLMDQINTATDPKAIADLQ 154

                         170       180       190
                  ....*....|....*....|....*....|....*
gi 556464597  182 NSIQLKSVMLNTLTTQWEMSVKAAEKRENALEAER 216
Cdd:pfam07996 155 ARIQAEQAMLQNEQTRLQMLQMLAEAEDRLAEQRR 189
 
Name Accession Description Interval E-value
T4SS pfam07996
Type IV secretion system proteins; Members of this family are components of the type IV ...
 22-216 6.10e-37

Type IV secretion system proteins; Members of this family are components of the type IV secretion system. They mediate intracellular transfer of macromolecules via a mechanism ancestrally related to that of bacterial conjugation machineries.


Pssm-ID: 429777 [Multi-domain]  Cd Length: 189  Bit Score: 128.23  E-value: 6.10e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556464597   22 GIPVaVDADPMrdVQWAQELKRWMETARHYQSQIQAYKDQLATATGVRDIADFVDQAKGLKADLEKLRKPGQALNDLLLS 101
Cdd:pfam07996   1 GIPV-IDAAAI--AQAIAQVVEAIAQLTQLKQQINQYKQQYNSLTGARGLGDILNNPALRNYLPADWQDIYDLVKSGGSY 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556464597  102 GGSSGQFDALYEKYKIFDTCNtaqSGSYANVCKQQVINKAIQLEQTDEVQNQVSQTLGEINSLSNRVALAKDSKESQDLA 181
Cdd:pfam07996  78 GSLSSAAQSLRDANKLYDVCT---DDDRADACQQAANKAAQDKAFAEQAYDTATQRLDQIQQLMDQINTATDPKAIADLQ 154

                         170       180       190
                  ....*....|....*....|....*....|....*
gi 556464597  182 NSIQLKSVMLNTLTTQWEMSVKAAEKRENALEAER 216
Cdd:pfam07996 155 ARIQAEQAMLQNEQTRLQMLQMLAEAEDRLAEQRR 189
VirB5_like cd14262
VirB5 protein family; This family contains VirB5 domains, including TraC, a VirB5 homolog ...
 39-197 1.11e-05

VirB5 protein family; This family contains VirB5 domains, including TraC, a VirB5 homolog encoded by the pKM101 plasmid, and similar proteins. VirB5 is one of 11 conserved proteins (VirB1-VirB11) in Agrobacterium tumefaciens, the causative agent of crown gall disease, that span the inner and the outer membrane, and is involved in type IV DNA secretion systems (T4SS) which mediate the translocation of virulence factors (proteins and/or DNA) from Gram-negative bacteria into eukaryotic cells. VirB5 assembles extracellular pili by interacting with several essential proteins. VirB2-VirB5 complex formation precedes incorporation into pili; it depends on the inner membrane protein VirB4 to interact directly with and stabilize VirB8 in order for VirB5 to bind to VirB8 and VirB10. Mutagenesis studies show that VirB5 proteins participate in protein-protein interactions important for pilus assembly and function.


Pssm-ID: 271354 [Multi-domain]  Cd Length: 173  Bit Score: 44.26  E-value: 1.11e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556464597  39 QELKRWMETARHYQSQIQAYKDQLATATGVRDIADFVDQAKGLKADLEKLRKPGQALNDLLLSGGSSGQFDalyEKYKIF 118
Cdd:cd14262    1 QQLAQLKQQLEQLKQQLEQAKKQYDSITGSRGLGDLLNNPNLRSLLPLDPQSIYSAAKGGGSSGSSGSLAA---IISQNE 77

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 556464597 119 DTCNTAQSGSYANVCKQQVINKAIQLEQTDEVQNQVSQTLGEINSLSNRVALAKDSKESQDLANSIQLKSVMLNTLTTQ 197
Cdd:cd14262   78 EERRLDSVGEAREAIDKRSQYAAQDKAVSLQAYEEAEQRLKQIQSLLQQINTTQDPKAIADLQARIQAEQAMIQNEQTK 156
 
Name Accession Description Interval E-value
T4SS pfam07996
Type IV secretion system proteins; Members of this family are components of the type IV ...
 22-216 6.10e-37

Type IV secretion system proteins; Members of this family are components of the type IV secretion system. They mediate intracellular transfer of macromolecules via a mechanism ancestrally related to that of bacterial conjugation machineries.


Pssm-ID: 429777 [Multi-domain]  Cd Length: 189  Bit Score: 128.23  E-value: 6.10e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556464597   22 GIPVaVDADPMrdVQWAQELKRWMETARHYQSQIQAYKDQLATATGVRDIADFVDQAKGLKADLEKLRKPGQALNDLLLS 101
Cdd:pfam07996   1 GIPV-IDAAAI--AQAIAQVVEAIAQLTQLKQQINQYKQQYNSLTGARGLGDILNNPALRNYLPADWQDIYDLVKSGGSY 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556464597  102 GGSSGQFDALYEKYKIFDTCNtaqSGSYANVCKQQVINKAIQLEQTDEVQNQVSQTLGEINSLSNRVALAKDSKESQDLA 181
Cdd:pfam07996  78 GSLSSAAQSLRDANKLYDVCT---DDDRADACQQAANKAAQDKAFAEQAYDTATQRLDQIQQLMDQINTATDPKAIADLQ 154

                         170       180       190
                  ....*....|....*....|....*....|....*
gi 556464597  182 NSIQLKSVMLNTLTTQWEMSVKAAEKRENALEAER 216
Cdd:pfam07996 155 ARIQAEQAMLQNEQTRLQMLQMLAEAEDRLAEQRR 189
VirB5_like cd14262
VirB5 protein family; This family contains VirB5 domains, including TraC, a VirB5 homolog ...
 39-197 1.11e-05

VirB5 protein family; This family contains VirB5 domains, including TraC, a VirB5 homolog encoded by the pKM101 plasmid, and similar proteins. VirB5 is one of 11 conserved proteins (VirB1-VirB11) in Agrobacterium tumefaciens, the causative agent of crown gall disease, that span the inner and the outer membrane, and is involved in type IV DNA secretion systems (T4SS) which mediate the translocation of virulence factors (proteins and/or DNA) from Gram-negative bacteria into eukaryotic cells. VirB5 assembles extracellular pili by interacting with several essential proteins. VirB2-VirB5 complex formation precedes incorporation into pili; it depends on the inner membrane protein VirB4 to interact directly with and stabilize VirB8 in order for VirB5 to bind to VirB8 and VirB10. Mutagenesis studies show that VirB5 proteins participate in protein-protein interactions important for pilus assembly and function.


Pssm-ID: 271354 [Multi-domain]  Cd Length: 173  Bit Score: 44.26  E-value: 1.11e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 556464597  39 QELKRWMETARHYQSQIQAYKDQLATATGVRDIADFVDQAKGLKADLEKLRKPGQALNDLLLSGGSSGQFDalyEKYKIF 118
Cdd:cd14262    1 QQLAQLKQQLEQLKQQLEQAKKQYDSITGSRGLGDLLNNPNLRSLLPLDPQSIYSAAKGGGSSGSSGSLAA---IISQNE 77

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 556464597 119 DTCNTAQSGSYANVCKQQVINKAIQLEQTDEVQNQVSQTLGEINSLSNRVALAKDSKESQDLANSIQLKSVMLNTLTTQ 197
Cdd:cd14262   78 EERRLDSVGEAREAIDKRSQYAAQDKAVSLQAYEEAEQRLKQIQSLLQQINTTQDPKAIADLQARIQAEQAMIQNEQTK 156
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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