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Conserved domains on  [gi|639337329|ref|WP_024600144|]
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MULTISPECIES: tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL [Pseudoalteromonas]

Protein Classification

tRNA (cytidine(34)-2'-O)-methyltransferase( domain architecture ID 10000715)

tRNA (cytidine(34)-2'-O)-methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine to the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA)

EC:  2.1.1.207
Gene Ontology:  GO:0003723|GO:0008173|GO:1904047|GO:0141098|GO:0001510
SCOP:  4002340

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-153 3.34e-105

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


:

Pssm-ID: 439989  Cd Length: 153  Bit Score: 296.95  E-value: 3.34e-105
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329   1 MLDIVLYQPEIPPNTGNIIRLCANTGYALHLIEPLGFEWDDKRVKRAGLDYHEFSHVQRHASFEDYLEKCKPKKVYACTT 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGGRLFLFTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 639337329  81 KGSQFHHEANYEAGDCLVFGPETRGLPDDMIQAlPKEQRVRIPMQANSRSMNLSNAVAVFVYESWRQLGFPNA 153
Cdd:COG0219   81 KGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAA-PADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
 
Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-153 3.34e-105

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 296.95  E-value: 3.34e-105
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329   1 MLDIVLYQPEIPPNTGNIIRLCANTGYALHLIEPLGFEWDDKRVKRAGLDYHEFSHVQRHASFEDYLEKCKPKKVYACTT 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGGRLFLFTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 639337329  81 KGSQFHHEANYEAGDCLVFGPETRGLPDDMIQAlPKEQRVRIPMQANSRSMNLSNAVAVFVYESWRQLGFPNA 153
Cdd:COG0219   81 KGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAA-PADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-153 8.37e-91

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 260.70  E-value: 8.37e-91
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329   1 MLDIVLYQPEIPPNTGNIIRLCANTGYALHLIEPLGFEWDDKRVKRAGLDYHEFSHVQRHASFEDYLEKCKPKKVYACTT 80
Cdd:PRK10358   1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQRLFALTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 639337329  81 KGSQFHHEANYEAGDCLVFGPETRGLPDDMIQALPKEQRVRIPMQANSRSMNLSNAVAVFVYESWRQLGFPNA 153
Cdd:PRK10358  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGA 153
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 4-148 6.52e-90

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 257.77  E-value: 6.52e-90
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329   4 IVLYQPEIPPNTGNIIRLCANTGYALHLIEPLGFEWDDKRVKRAGLDYHEFSHVQRHASFEDYLEKCKPKKVYACTTKGS 83
Cdd:cd18094    2 IVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALPPGRLFLFTTKGT 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 639337329  84 QFHHEANYEAGDCLVFGPETRGLPDDMIQALPkEQRVRIPMQANSRSMNLSNAVAVFVYESWRQL 148
Cdd:cd18094   82 KPYTDFDFKPGDYLVFGRESTGLPEEILDAFP-DRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 1-153 1.05e-80

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 235.00  E-value: 1.05e-80
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329    1 MLDIVLYQPEIPPNTGNIIRLCANTGYALHLIEPLGFEWDDKRVKRAGLDYHEFSHVQRHASFEDYLEKCKPKKVYACTT 80
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEKPQKLFALTK 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 639337329   81 KGSQFHHEANYEAGDCLVFGPETRGLPDDMIQALpKEQRVRIPMQANSRSMNLSNAVAVFVYESWRQLGFPNA 153
Cdd:TIGR00185  81 KGTPAHSAVTYQDGDYLMFGPETRGLPQSILDNL-MEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGYPGL 152
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 1-142 2.52e-45

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 144.98  E-value: 2.52e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329    1 MLDIVLYQPEIPPNTGNIIRLCANTG-YALHLIEPLGFEWDDKRVKRAGLDYHEFSHVQRHASFEDYLEKCKPK--KVYA 77
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGaDGLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAgyAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 639337329   78 CTTKGSQFhHEANYEAGDCLVFGPETRGLPDDMIQALPkeQRVRIPMQANSRSMNLSNAVAVFVY 142
Cdd:pfam00588  81 TSLGGKSL-YEIDFPKKVALVFGNEGTGLSNEVLELCD--RLVRIPMSGDVESLNVAVAAAIALY 142
 
Name Accession Description Interval E-value
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 1-153 3.34e-105

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 296.95  E-value: 3.34e-105
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329   1 MLDIVLYQPEIPPNTGNIIRLCANTGYALHLIEPLGFEWDDKRVKRAGLDYHEFSHVQRHASFEDYLEKCKPKKVYACTT 80
Cdd:COG0219    1 MLHIVLYEPEIPQNTGNIIRLCAATGARLHLIEPLGFSLDDKKLKRAGLDYWELVDLTVHDSWEAFLEALPGGRLFLFTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 639337329  81 KGSQFHHEANYEAGDCLVFGPETRGLPDDMIQAlPKEQRVRIPMQANSRSMNLSNAVAVFVYESWRQLGFPNA 153
Cdd:COG0219   81 KGTKPYTDVDYQPGDVLLFGRETAGLPEEVLAA-PADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 1-153 8.37e-91

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 260.70  E-value: 8.37e-91
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329   1 MLDIVLYQPEIPPNTGNIIRLCANTGYALHLIEPLGFEWDDKRVKRAGLDYHEFSHVQRHASFEDYLEKCKPKKVYACTT 80
Cdd:PRK10358   1 MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYAAFLEAENPQRLFALTT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 639337329  81 KGSQFHHEANYEAGDCLVFGPETRGLPDDMIQALPKEQRVRIPMQANSRSMNLSNAVAVFVYESWRQLGFPNA 153
Cdd:PRK10358  81 KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGA 153
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 4-148 6.52e-90

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 257.77  E-value: 6.52e-90
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329   4 IVLYQPEIPPNTGNIIRLCANTGYALHLIEPLGFEWDDKRVKRAGLDYHEFSHVQRHASFEDYLEKCKPKKVYACTTKGS 83
Cdd:cd18094    2 IVLYEPEIPQNTGNIARTCAATGARLHLIEPLGFSLDDKRLKRAGLDYWDHVDLTVHDSWEAFLEALPPGRLFLFTTKGT 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 639337329  84 QFHHEANYEAGDCLVFGPETRGLPDDMIQALPkEQRVRIPMQANSRSMNLSNAVAVFVYESWRQL 148
Cdd:cd18094   82 KPYTDFDFKPGDYLVFGRESTGLPEEILDAFP-DRRLRIPMRPKVRSLNLSNSVAIVLYEALRQL 145
tRNA_yibK_trmL TIGR00185
tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2 ...
 1-153 1.05e-80

tRNA (cytidine(34)-2'-O)-methyltransferase; TrmL (previously YibK) is responsible for 2'-O-methylation at tRNA(Leu) position 34. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 211559  Cd Length: 153  Bit Score: 235.00  E-value: 1.05e-80
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329    1 MLDIVLYQPEIPPNTGNIIRLCANTGYALHLIEPLGFEWDDKRVKRAGLDYHEFSHVQRHASFEDYLEKCKPKKVYACTT 80
Cdd:TIGR00185   1 MLNIVLYEPEIPPNTGNIARTCAATGTRLHLIEPLGFFLDDKRLKRAGLDYWEFVQVFYHKSWEEFLEAEKPQKLFALTK 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 639337329   81 KGSQFHHEANYEAGDCLVFGPETRGLPDDMIQALpKEQRVRIPMQANSRSMNLSNAVAVFVYESWRQLGFPNA 153
Cdd:TIGR00185  81 KGTPAHSAVTYQDGDYLMFGPETRGLPQSILDNL-MEQKIRIPMTNDVRSLNLSNAVAIVVYEAWRQLGYPGL 152
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 1-142 2.52e-45

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 144.98  E-value: 2.52e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329    1 MLDIVLYQPEIPPNTGNIIRLCANTG-YALHLIEPLGFEWDDKRVKRAGLDYHEFSHVQRHASFEDYLEKCKPK--KVYA 77
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGaDGLILVEPRSADPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAgyAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 639337329   78 CTTKGSQFhHEANYEAGDCLVFGPETRGLPDDMIQALPkeQRVRIPMQANSRSMNLSNAVAVFVY 142
Cdd:pfam00588  81 TSLGGKSL-YEIDFPKKVALVFGNEGTGLSNEVLELCD--RLVRIPMSGDVESLNVAVAAAIALY 142
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 4-143 3.94e-32

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 111.55  E-value: 3.94e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329   4 IVLYQPEIPPNTGNIIRLCANTGY-ALHLIEPLGFEWDDKrVKRAGLDYHEFSHVQRHASFEDYLEKCKPKK--VYAC-T 79
Cdd:cd18082    2 LVLDNIRSPGNIGSIFRTADAFGVeKIYLVGPTADIFDPK-AKRTSRGTHFSVPYERVDNFEEYLKSLKEEGypLIGLeL 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 639337329  80 TKGSQFHHEANYEA--GDCLVFGPETRGLPDDMIQALpkEQRVRIPMQANSRSMNLSNAVAVFVYE 143
Cdd:cd18082   81 TDGSIPLSEFKFKKpkKIALIFGNERSGLSPELLALC--DYTVHIPMSGKNSSLNVSVATGIALYE 144
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 4-143 4.70e-16

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 70.22  E-value: 4.70e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329   4 IVLYQPEIPPNTGNIIRLCANTGYA-LHLIEPLGFEWDDKRVKRAGLDYHEFSHVQRHASFEDYLEKCKpkKVYACTTKG 82
Cdd:cd18093    3 VVLVEPQHPGNIGAVARAMKNFGLSdLRLVNPRCDDPEEAARALASGAEDVLENAKVYDTLEEALADCD--LVVATTARP 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 639337329  83 SQFHH------EANYEAGD-------CLVFGPETRGLP-DDMIQAlpkEQRVRIPMQANSRSMNLSNAVAVFVYE 143
Cdd:cd18093   81 RGLRRplltprELAELLKEhaaggkvALVFGREDSGLTnEELALC---DLLVTIPTNPEYPSLNLAQAVAIVLYE 152
SpoU-like_RNA-MTase cd18104
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 4-147 1.55e-15

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349977  Cd Length: 146  Bit Score: 68.76  E-value: 1.55e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329   4 IVLYQPEIPPNTGNIIRLCANTGYALHLIEPLGFEWDDKRVKRAGLDYhEFSHVQRHASFEDYLEKCKPKK--VYACTTK 81
Cdd:cd18104    3 LVLEGLEKPGNLGAILRSADAAGADAVILCDPKTDLFNPNVIRASLGA-VFTLPVAEASSEETLAWLKENKirILATTPK 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 639337329  82 GSQFHHEANYEAGDCLVFGPETRGLPDDMIQAlpKEQRVRIPMQANSRSMNLSNAVAVFVYESWRQ 147
Cdd:cd18104   82 AEKLYYEADLRGPIAIVVGSESEGLSEFWLKA--ADERVRIPMAGQADSLNVSVAAAILLYEALRQ 145
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 59-147 3.89e-15

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 70.22  E-value: 3.89e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329  59 RHASFEDYLEKCKPK--KVYACTTKGSQFHHEANYEAGDCLVFGPETRGLPDDMIQALpkEQRVRIPMQANSRSMNLSNA 136
Cdd:COG0566  173 RVTNLADALEELKEAgfWVVGTDLDGEASLYEVDLPGPVALVLGNEGKGLSRLVLEAC--DELVRIPMAGGVESLNVAVA 250

                         90
                 ....*....|.
gi 639337329 137 VAVFVYESWRQ 147
Cdd:COG0566  251 AAILLYEAARQ 261
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 4-149 2.45e-13

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 64.75  E-value: 2.45e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329   4 IVLYQPEIPPNTGNIIRLCANTGYA-LHLIEPLGFEwDDKRVKRAG-----LDyhefsHVQRHASFEDYLEKCKpkKVYA 77
Cdd:COG0565    6 VVLVEPQHPGNIGAAARAMKNFGLSdLRLVNPRGWP-DEEARAMASgaddvLE-----NARVVDTLEEALADCD--LVVG 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329  78 CTTKGSQFHH----------EANYEAGD---CLVFGPETRGLPDD---MIQALpkeqrVRIPMQANSRSMNLSNAVAVFV 141
Cdd:COG0565   78 TTARPRELRKpvltpreaaeELLARAAGgkvALVFGRERSGLTNEelaLCDAL-----VTIPTNPEYPSLNLAQAVQVVA 152


                 ....*...
gi 639337329 142 YEsWRQLG 149
Cdd:COG0565  153 YE-LRKAA 159
SpoU-like cd18097
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 14-143 2.58e-11

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349970  Cd Length: 144  Bit Score: 57.57  E-value: 2.58e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329  14 NTGNIIRLCANTGYA-LHL--IEPLGfewDDKRVKRAGLDYHEFSHVQRHASFEDYLEKCKPKKVYAC---TTKGSQFHH 87
Cdd:cd18097   13 NVGSIFRTADAAGVEkIYLcgYTPTP---PHPKVAKTALGAEESVPWEHFEDTLEAVRALKARGYTIIaleTTENSVPLH 89

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 639337329  88 EANYEAGDCLVFGPETRGLPDDMIQALpkEQRVRIPMQANSRSMNLSNAVAVFVYE 143
Cdd:cd18097   90 EARLPRPLALVLGNEVRGVSPEVLALC--DAVVEIPMFGRKNSLNVAVAAAVALYE 143
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 12-143 4.60e-11

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 57.05  E-value: 4.60e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329  12 PPNTGNIIRLCAntgyALhlieplGFE-----------WDDKRVkRAGLDYHEFSHVQRHASFEDYLEKCKPK--KVYAC 78
Cdd:cd18095   11 PGNLGTIIRTAA----AF------GVDgvilskgtvdpYNPKVV-RASMGALFRLPIIRVDDLEELLEELKEAgyKVVAA 79

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 639337329  79 TTKGSQFHHEANYEAGDCLVFGPETRGLPDDMIQALPKeqRVRIPMQANSRSMNLSNAVAVFVYE 143
Cdd:cd18095   80 DLDGGSSLYDLDLPKPVALVLGNEGNGLSDELLELCDK--RVTIPMSGGVESLNVAVAAAILLYE 142
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 14-148 8.65e-11

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 56.71  E-value: 8.65e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329  14 NTGNIIRLCANTGYA-LHLIEPLGfewDDKRVKRAGLDYHEFSHVQRHASFEDYLEKCKPK--KVYA-CTTKGSQFHHEA 89
Cdd:cd18092   29 NASAVLRTCDAFGVQdVHVIEPKN---KFKKNRGVAMGADKWLDVHRHDSTEECLAALKEQgyRIVAtSLSPDAVPLDEL 105

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 639337329  90 NYEAGDCLVFGPETRGLPDDMIQAlpKEQRVRIPMQANSRSMNLSNAVAVFVYESWRQL 148
Cdd:cd18092  106 DYTRPTALVFGNEKDGVSEEALAA--ADERVYIPMYGFVESLNVSVAAAIILYELQRQR 162
SpoU-like_RlmB cd18103
SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the ...
 67-143 2.76e-08

SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349976  Cd Length: 143  Bit Score: 49.69  E-value: 2.76e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329  67 LEKCKPK--KVYACTTKGSQFHHEANYEAGDCLVFGPETRGlpddmIQALPKE---QRVRIPMQANSRSMNLSNAVAVFV 141
Cdd:cd18103   66 LEELKEAgfWIYGADGEAEKSLYDVDLTGPLALVLGSEGKG-----LRRLVLKncdFLVKIPMAGKVDSLNVSVAAGIIL 140


                 ..
gi 639337329 142 YE 143
Cdd:cd18103  141 YE 142
SpoU-like_AviRb cd18107
SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes ...
 2-143 1.14e-07

SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA. AviRb is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349980  Cd Length: 148  Bit Score: 48.15  E-value: 1.14e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329   2 LDIVLYQPEIPPNTGNIIRLCANTGyaLHLIEPLGFEWD--DKRVKRAGLDyHEFS----HVQRHASFEDYLE----KCK 71
Cdd:cd18107    1 LVVLFDRPTSPGNLGTIIRSADALG--AHGLVVTGHAADvyDPKTIRASTG-SLFSlpivRVPSNRELEDWVEarkaRGT 77

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 639337329  72 PKKVYACTTKGSQFHHEANYEAGDCLVFGPETRGLpddmiQALPKE---QRVRIPMQANSRSMNLSNAVAVFVYE 143
Cdd:cd18107   78 PLQLVGTDEHGDHQIADHDFTRPTLLLIGNETAGL-----SAAWRElcdETVSIPMAGSASSLNAACAASIFLYE 147
PRK10864 PRK10864
putative methyltransferase; Provisional
 12-147 1.38e-05

putative methyltransferase; Provisional


Pssm-ID: 236779 [Multi-domain]  Cd Length: 346  Bit Score: 43.61  E-value: 1.38e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329  12 PPNTGNIIRLCANTGYALHLIEPLGFEWDDKRVKRA--GLDyhefsHVQ--RHASFEDYLEKCKpKKVYACTT----KGS 83
Cdd:PRK10864 209 PHNLGGIMRSCAHFGVKGVVVQDAALLESGAAIRTAegGAE-----HVQpiTGDSFVDVLDDFR-QAGYTIVTtsshKGT 282

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 639337329  84 QFHhEANYEAGDCLVFGPETRGLPDDMIQalPKEQRVRIPMQANSRSMNLSNAVAVFVYESWRQ 147
Cdd:PRK10864 283 PLF-KASLPAKMVLVLGQEYDGLSDAARQ--QGDLSVSIDGTGNVESLNVSVATGVLLAEWWRQ 343
SpoU-like_RNMTL1 cd18106
SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) ...
 12-143 1.14e-04

SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) catalyzes the methylation of 2'-O-ribose residues G1370 to GmG residue of the mitochondrial 16S rRNA. RNMTL1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349979  Cd Length: 148  Bit Score: 39.93  E-value: 1.14e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329  12 PPNTGNIIRLCANTG-YALHLIEPLGFEWDDKrVKRAGLDYHEFSHVQRHASFED---YLEKCKpKKVYACTTKGSQFHH 87
Cdd:cd18106   11 PGNLGSILRSAAAAGcSKVLLTKGCVDPWEPK-VLRAGAGAHFRLPIYGNLSWDDipnYLPDDS-KVFLADNNLPVQPYD 88

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 639337329  88 EANYEAGDC-LVFGPETRGLPDD--MIQALPKEQRVRIPMQANSRSMNLSNAVAVFVYE 143
Cdd:cd18106   89 SIDYCSGPIvLVIGGETHGVSPEayKLAHSTGGSRVHIPLANGVDSLNSAVAASILLFE 147
SpoU-like_RNA-MTase cd18109
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 54-143 1.29e-04

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349982  Cd Length: 141  Bit Score: 39.83  E-value: 1.29e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329  54 FSHVQRH-ASFEDYLEKCKPK-KVYACTTKGSQFHhEANYEAGDCLVFGPETRGLPDDMIQALpkEQRVRIPMQAN-SRS 130
Cdd:cd18109   51 IFRVQIHyTDLPALLAELKKAfPVYGADLDGENLY-EFTLPEKGILVMGNEGHGISPEVLKLL--THRLTIPRYGKgAES 127

                         90
                 ....*....|...
gi 639337329 131 MNLSNAVAVFVYE 143
Cdd:cd18109  128 LNVAVATAILLSE 140
SpoU-like_TRM3-like cd18091
SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O) ...
 96-143 1.92e-04

SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O)-methyltransferase TRM3 catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in various tRNAs. TRM3 is similar to C-terminal domain of TAR (HIV-1) RNA binding protein 1 (TARBP1), a protein binding to TAR, which functions as a RNA regulatory signal by forming a stable stem-loop structure to which transactivator protein Tat binds. The role of TARBP1 is believed to be to disengage RNA polymerase II from TAR during transcriptional elongation. TRM3 and the C-terminal methyltransferase domain of TARBP1 are members of the SPOUT methyltransferase superfamily.


Pssm-ID: 349964  Cd Length: 145  Bit Score: 39.05  E-value: 1.92e-04
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 639337329  96 CLVFGPETRGLPDDMIQALpkEQRVRIPMQANSRSMNLSNAVAVFVYE 143
Cdd:cd18091   99 VLVLGNEKEGIPAELLSLL--DVCVEIPQLGVIRSLNVHVSGAIAIYE 144
rRNA_methyl_1 TIGR00050
RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of ...
 97-143 3.45e-04

RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases, and is now characterized, in E. coli, as a tRNA:Cm32/Um32 methyltransferase. It may be named TrMet(Xm32), or TrmJ, according to the nomenclature style chosen [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272876 [Multi-domain]  Cd Length: 233  Bit Score: 39.30  E-value: 3.45e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*..
gi 639337329   97 LVFGPETRGLPDDMIQALpkEQRVRIPMQANSRSMNLSNAVAVFVYE 143
Cdd:TIGR00050 108 LVFGREDSGLTNEELQKC--HVLVSIPTSEEYPSLNLSHAVAVILYE 152
SpoU-like cd18098
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 4-142 3.51e-04

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349971  Cd Length: 138  Bit Score: 38.40  E-value: 3.51e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329   4 IVLYQPEIPPNTGNIIRLCANTGYA-LHLIeplgfewdDKRVKRagldYHEFSHVQRHASFEDYLEKCKPKKVYACTTKG 82
Cdd:cd18098    3 IGIENPKTPKNVGTLWRAAGCLGASfVFTI--------GARYKR----RKQSTDTSKAWRHIPLFHYDDLEDLAEPLPFG 70

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 639337329  83 SQ---------------FHHEANYeagdCLVFGPETRGLPDDMIQALpkEQRVRIPMQansRSMNLSNAVAVFVY 142
Cdd:cd18098   71 AVlvaveltegatplpdFKHPDRA----VYLLGPEDGGLPQEVLARC--HHVVYLPTR---GSLNVAVAGSIVLY 136
PRK11081 PRK11081
tRNA guanosine-2'-O-methyltransferase; Provisional
 88-147 1.87e-03

tRNA guanosine-2'-O-methyltransferase; Provisional


Pssm-ID: 236837 [Multi-domain]  Cd Length: 229  Bit Score: 37.28  E-value: 1.87e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 639337329  88 EANYEAGDCLVFGPETRGLPDDMIQAlpKEQRVRIPMQANSRSMNLSNAVAVFVYESWRQ 147
Cdd:PRK11081 107 EIDYTRPTCILMGQEKTGISQEALAL--ADQDIIIPMIGMVQSLNVSVASALILYEAQRQ 164
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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