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Conserved domains on  [gi|640232214|ref|WP_024830804|]
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MULTISPECIES: phosphodiester glycosidase family protein [Methylobacterium]

Protein Classification

phosphodiester glycosidase family protein( domain architecture ID 10007847)

phosphodiester glycosidase family protein similar to mammalian N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase, which catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step; also includes Escherichia coli YigE

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
YigE COG3698
Uncharacterized conserved protein YigE, DUF2233 family [Function unknown];
35-249 3.84e-127

Uncharacterized conserved protein YigE, DUF2233 family [Function unknown];


:

Pssm-ID: 442913  Cd Length: 239  Bit Score: 359.91  E-value: 3.84e-127
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 640232214  35 CRAVEAQGETFTVCTIDLRKQRLRLFWLQDDGKPYGALGTLAE---KQGGRLAFAMNAGMYDKDQAPVGLYVEDGHELKR 111
Cdd:COG3698   17 CEDLTFEGARYTVCTVDPAREDLRLFWKDDDGEPYGSFSALAAalaAKGKRLAFAMNAGMYHPDRSPVGLYVENGRELVP 96
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 640232214 112 VSTTDGPGNFHLKPNGIFWVKGERAGVVDTARYLRAKIRPDFATQSGPMLVIDGQIHPKIAADGPSQKIRNGVGVPGDGH 191
Cdd:COG3698   97 LNTASGPGNFGLLPNGVFYLGDGRAGVIETRAFLAAGPKCRYATQSGPMLVIDGALHPRFLPDSTSRKIRNGVGVSADGR 176
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 640232214 192 VaIFAISERPVTFGAFARLFRDDLGCRNALFLDGSVSSLYAPNLGRSDISRPLGPLVG 249
Cdd:COG3698  177 V-VFAISEEPVNFYDFARLFRDRLGCPNALYLDGTISRLYAPELGRNDQGFPFGPIIG 233
 
Name Accession Description Interval E-value
YigE COG3698
Uncharacterized conserved protein YigE, DUF2233 family [Function unknown];
35-249 3.84e-127

Uncharacterized conserved protein YigE, DUF2233 family [Function unknown];


Pssm-ID: 442913  Cd Length: 239  Bit Score: 359.91  E-value: 3.84e-127
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 640232214  35 CRAVEAQGETFTVCTIDLRKQRLRLFWLQDDGKPYGALGTLAE---KQGGRLAFAMNAGMYDKDQAPVGLYVEDGHELKR 111
Cdd:COG3698   17 CEDLTFEGARYTVCTVDPAREDLRLFWKDDDGEPYGSFSALAAalaAKGKRLAFAMNAGMYHPDRSPVGLYVENGRELVP 96
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 640232214 112 VSTTDGPGNFHLKPNGIFWVKGERAGVVDTARYLRAKIRPDFATQSGPMLVIDGQIHPKIAADGPSQKIRNGVGVPGDGH 191
Cdd:COG3698   97 LNTASGPGNFGLLPNGVFYLGDGRAGVIETRAFLAAGPKCRYATQSGPMLVIDGALHPRFLPDSTSRKIRNGVGVSADGR 176
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 640232214 192 VaIFAISERPVTFGAFARLFRDDLGCRNALFLDGSVSSLYAPNLGRSDISRPLGPLVG 249
Cdd:COG3698  177 V-VFAISEEPVNFYDFARLFRDRLGCPNALYLDGTISRLYAPELGRNDQGFPFGPIIG 233
NAGPA pfam09992
Phosphodiester glycosidase; This is a family conserved from bacteria to humans. The structure ...
85-229 2.13e-23

Phosphodiester glycosidase; This is a family conserved from bacteria to humans. The structure of a member from Bacteroides has been crystallized and modelled onto the luminal region of the human member of the family, the transmembrane glycoprotein N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase. There is some conservation of potentially functional residues, implying that in the bacterial members this family acts in some way as a phosphodiester glycosidase. The human protein is also present, so the eukaryotic members are likely to be catalysing the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides.


Pssm-ID: 430971  Cd Length: 169  Bit Score: 92.77  E-value: 2.13e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 640232214   85 FAMNAGMYDKDQ-APVGLYVEDGHELKRVSTTDGPGNFHLKPNGIFWVKGERAGVVDtarylrakirPDFATQSGPMLVI 163
Cdd:pfam09992   5 AAVNGGFFDPGSgGPLGLVISNGKVLGLLNGGRAVGAFALTPDGVLVITLNPLDFYD----------LSEAVGAGPLLVK 74
                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 640232214  164 DGQIHPKIAADGPSQKIRNGVGVPGDGHVAIFAISER---PVTFGAFARLFRdDLGCRNALFLDGSVSS 229
Cdd:pfam09992  75 DGKIVPTSSDGGWGRAPRTAIGITADGTILLVVVDGRqsiGATLKELAQLLK-RLGAVNALNLDGGGST 142
 
Name Accession Description Interval E-value
YigE COG3698
Uncharacterized conserved protein YigE, DUF2233 family [Function unknown];
35-249 3.84e-127

Uncharacterized conserved protein YigE, DUF2233 family [Function unknown];


Pssm-ID: 442913  Cd Length: 239  Bit Score: 359.91  E-value: 3.84e-127
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 640232214  35 CRAVEAQGETFTVCTIDLRKQRLRLFWLQDDGKPYGALGTLAE---KQGGRLAFAMNAGMYDKDQAPVGLYVEDGHELKR 111
Cdd:COG3698   17 CEDLTFEGARYTVCTVDPAREDLRLFWKDDDGEPYGSFSALAAalaAKGKRLAFAMNAGMYHPDRSPVGLYVENGRELVP 96
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 640232214 112 VSTTDGPGNFHLKPNGIFWVKGERAGVVDTARYLRAKIRPDFATQSGPMLVIDGQIHPKIAADGPSQKIRNGVGVPGDGH 191
Cdd:COG3698   97 LNTASGPGNFGLLPNGVFYLGDGRAGVIETRAFLAAGPKCRYATQSGPMLVIDGALHPRFLPDSTSRKIRNGVGVSADGR 176
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 640232214 192 VaIFAISERPVTFGAFARLFRDDLGCRNALFLDGSVSSLYAPNLGRSDISRPLGPLVG 249
Cdd:COG3698  177 V-VFAISEEPVNFYDFARLFRDRLGCPNALYLDGTISRLYAPELGRNDQGFPFGPIIG 233
NAGPA pfam09992
Phosphodiester glycosidase; This is a family conserved from bacteria to humans. The structure ...
85-229 2.13e-23

Phosphodiester glycosidase; This is a family conserved from bacteria to humans. The structure of a member from Bacteroides has been crystallized and modelled onto the luminal region of the human member of the family, the transmembrane glycoprotein N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase. There is some conservation of potentially functional residues, implying that in the bacterial members this family acts in some way as a phosphodiester glycosidase. The human protein is also present, so the eukaryotic members are likely to be catalysing the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides.


Pssm-ID: 430971  Cd Length: 169  Bit Score: 92.77  E-value: 2.13e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 640232214   85 FAMNAGMYDKDQ-APVGLYVEDGHELKRVSTTDGPGNFHLKPNGIFWVKGERAGVVDtarylrakirPDFATQSGPMLVI 163
Cdd:pfam09992   5 AAVNGGFFDPGSgGPLGLVISNGKVLGLLNGGRAVGAFALTPDGVLVITLNPLDFYD----------LSEAVGAGPLLVK 74
                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 640232214  164 DGQIHPKIAADGPSQKIRNGVGVPGDGHVAIFAISER---PVTFGAFARLFRdDLGCRNALFLDGSVSS 229
Cdd:pfam09992  75 DGKIVPTSSDGGWGRAPRTAIGITADGTILLVVVDGRqsiGATLKELAQLLK-RLGAVNALNLDGGGST 142
NAGPA COG4632
Sugar-P-sugar glycosidase (uncovering enzyme, UCE), NAGPA superfamily [Carbohydrate transport ...
90-232 2.87e-10

Sugar-P-sugar glycosidase (uncovering enzyme, UCE), NAGPA superfamily [Carbohydrate transport and metabolism];


Pssm-ID: 443670 [Multi-domain]  Cd Length: 310  Bit Score: 59.45  E-value: 2.87e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 640232214  90 GMYDKDQAPVGLYVEDGHELKRVSTTDGPGNFHLKPNGIFWVKGEragVVDTARYLRAKIRpdFATQSGPMLVIDGQIHP 169
Cdd:COG4632  141 GLFDPGGKPTGIIISNGKVISPNKDGPARDVLGIDKDGKLIVGDP---VTIDLEKLPAGVE--EAVGGGPLLVKNGKVVV 215
                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 640232214 170 KIAADGPSQKI--RNGVGVPGDGHVAIFAISERP-----VTFGAFARLFRDdLGCRNALFLD-GSVSSLYA 232
Cdd:COG4632  216 DADEAAFGNGRapRTAIGITADGTLLLVVVDGRQpgsigATLAELAQLLKE-LGAVDALNLDgGGSTTLVY 285
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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