NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|647497609|ref|WP_025809149|]
View 

MULTISPECIES: type II secretion system minor pseudopilin GspH [Pseudomonas]

Protein Classification

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
typeII_sec_gspH super family cl46935
type II secretion system protein H; This model represents GspH, protein H of the main terminal ...
 5-149 3.34e-29

type II secretion system protein H; This model represents GspH, protein H of the main terminal branch of the general secretion pathway, also called type II secretion. It transports folded proteins across the bacterial outer membrane and is widely distributed in Gram-negative pathogens. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


The actual alignment was detected with superfamily member TIGR01708:

Pssm-ID: 130769 [Multi-domain]  Cd Length: 143  Bit Score: 104.18  E-value: 3.34e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 647497609    5 RQQGFTLIELMVVLVIIGIASAAISLSIRP-DPLQGLRKDAERLAQLLQVAQAEARVDGRPISWRADAKGFAFSRPSDDG 83
Cdd:TIGR01708   2 RQSGFTLIELLVVLAIMGLVAAAAALSLVShYGTKSLDQVAGRLAARLRLAQTSARATGRPVLLRLGSRGIRFGERASLA 81

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 647497609   84 RGLDqfkddpQLRPRPWESSSIKVRI-EPGPRLVLNAEWIDAPLQVQLSDGQHSLTVQRTATGVVRV 149
Cdd:TIGR01708  82 LPVD------EVKMTDWTGQETVVADrQTVLTVLFLPDGRASPMDMTLQRKGRSVTVAVNWLGGLRL 142
 
Name Accession Description Interval E-value
typeII_sec_gspH TIGR01708
type II secretion system protein H; This model represents GspH, protein H of the main terminal ...
 5-149 3.34e-29

type II secretion system protein H; This model represents GspH, protein H of the main terminal branch of the general secretion pathway, also called type II secretion. It transports folded proteins across the bacterial outer membrane and is widely distributed in Gram-negative pathogens. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


Pssm-ID: 130769 [Multi-domain]  Cd Length: 143  Bit Score: 104.18  E-value: 3.34e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 647497609    5 RQQGFTLIELMVVLVIIGIASAAISLSIRP-DPLQGLRKDAERLAQLLQVAQAEARVDGRPISWRADAKGFAFSRPSDDG 83
Cdd:TIGR01708   2 RQSGFTLIELLVVLAIMGLVAAAAALSLVShYGTKSLDQVAGRLAARLRLAQTSARATGRPVLLRLGSRGIRFGERASLA 81

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 647497609   84 RGLDqfkddpQLRPRPWESSSIKVRI-EPGPRLVLNAEWIDAPLQVQLSDGQHSLTVQRTATGVVRV 149
Cdd:TIGR01708  82 LPVD------EVKMTDWTGQETVVADrQTVLTVLFLPDGRASPMDMTLQRKGRSVTVAVNWLGGLRL 142
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
3-70 4.78e-13

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 60.25  E-value: 4.78e-13
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 647497609   3 PSRQQGFTLIELMVVLVIIGIASAAISLSIRpDPLQG--LRKDAERLAQLLQVAQAEARVDGRPISWRAD 70
Cdd:COG4970    5 RRRQRGFTLIELLVVLAILAILAAIAVPSFS-SLIARqrLRAAANELAAALRLARSEAIRRGQPVTLSPS 73
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 4-29 2.96e-08

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 46.98  E-value: 2.96e-08
                          10        20
                  ....*....|....*....|....*.
gi 647497609    4 SRQQGFTLIELMVVLVIIGIASAAIS 29
Cdd:pfam07963   2 RKQRGFTLIELLVALAILAILLAAAL 27
PRK10574 PRK10574
putative major pilin subunit; Provisional
 5-26 9.99e-08

putative major pilin subunit; Provisional


Pssm-ID: 236718 [Multi-domain]  Cd Length: 146  Bit Score: 48.11  E-value: 9.99e-08
                         10        20
                 ....*....|....*....|..
gi 647497609   5 RQQGFTLIELMVVLVIIGIASA 26
Cdd:PRK10574   3 KQRGFTLIELMVVIAIIAILSA 24
pilin_ComGC NF040999
competence type IV pilus major pilin ComGC; ComGC, encoded in the comG operon, is the major ...
 6-23 1.83e-04

competence type IV pilus major pilin ComGC; ComGC, encoded in the comG operon, is the major pilin of a type IV pilus involved in natural transformation of monoderm bacteria (those lacking an outer membrane) such as Bacillus subtilis and Streptococcus pneumoniae. In the seed alignment, Bacillus proteins have a pair of Cys residues likely to form a disulfide bond while Streptococcus proteins lack Cys residues.


Pssm-ID: 468929  Cd Length: 84  Bit Score: 38.26  E-value: 1.83e-04
                         10
                 ....*....|....*...
gi 647497609   6 QQGFTLIELMVVLVIIGI 23
Cdd:NF040999   1 VKGFTLIEMLIVLLIISV 18
 
Name Accession Description Interval E-value
typeII_sec_gspH TIGR01708
type II secretion system protein H; This model represents GspH, protein H of the main terminal ...
 5-149 3.34e-29

type II secretion system protein H; This model represents GspH, protein H of the main terminal branch of the general secretion pathway, also called type II secretion. It transports folded proteins across the bacterial outer membrane and is widely distributed in Gram-negative pathogens. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


Pssm-ID: 130769 [Multi-domain]  Cd Length: 143  Bit Score: 104.18  E-value: 3.34e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 647497609    5 RQQGFTLIELMVVLVIIGIASAAISLSIRP-DPLQGLRKDAERLAQLLQVAQAEARVDGRPISWRADAKGFAFSRPSDDG 83
Cdd:TIGR01708   2 RQSGFTLIELLVVLAIMGLVAAAAALSLVShYGTKSLDQVAGRLAARLRLAQTSARATGRPVLLRLGSRGIRFGERASLA 81

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 647497609   84 RGLDqfkddpQLRPRPWESSSIKVRI-EPGPRLVLNAEWIDAPLQVQLSDGQHSLTVQRTATGVVRV 149
Cdd:TIGR01708  82 LPVD------EVKMTDWTGQETVVADrQTVLTVLFLPDGRASPMDMTLQRKGRSVTVAVNWLGGLRL 142
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
3-70 4.78e-13

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 60.25  E-value: 4.78e-13
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 647497609   3 PSRQQGFTLIELMVVLVIIGIASAAISLSIRpDPLQG--LRKDAERLAQLLQVAQAEARVDGRPISWRAD 70
Cdd:COG4970    5 RRRQRGFTLIELLVVLAILAILAAIAVPSFS-SLIARqrLRAAANELAAALRLARSEAIRRGQPVTLSPS 73
PulG COG2165
Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, ...
 1-61 2.95e-12

Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 441768 [Multi-domain]  Cd Length: 99  Bit Score: 59.16  E-value: 2.95e-12
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 647497609   1 MTPSRQQGFTLIELMVVLVIIGIASAAISLSirpdpLQGLRKDAERLAQLLQVAQAEARVD 61
Cdd:COG2165    5 RRRRRQRGFTLIELLVVIAIIGILAALALPA-----LQGARERARRAELRSNLRQIQQALE 60
PilE COG4968
Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];
1-59 7.78e-11

Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];


Pssm-ID: 443994 [Multi-domain]  Cd Length: 124  Bit Score: 55.85  E-value: 7.78e-11
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 647497609   1 MTPSRQQGFTLIELMVVLVIIGIAsAAISLSIrpdpLQGLR---KDAERLAQLLQVAQAEAR 59
Cdd:COG4968    4 RMRRRQRGFTLIELMIVVAIIGIL-AAIAIPS----YQDYVeraRRAEAKAALLELAQAQER 60
PilA COG4969
Type IV pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures];
5-28 1.03e-08

Type IV pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures];


Pssm-ID: 443995 [Multi-domain]  Cd Length: 134  Bit Score: 50.48  E-value: 1.03e-08
                         10        20
                 ....*....|....*....|....*
gi 647497609   5 RQQGFTLIELMVVLVIIGI-ASAAI 28
Cdd:COG4969    4 KQKGFTLIELMIVVAIIGIlAAIAI 28
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 4-29 2.96e-08

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 46.98  E-value: 2.96e-08
                          10        20
                  ....*....|....*....|....*.
gi 647497609    4 SRQQGFTLIELMVVLVIIGIASAAIS 29
Cdd:pfam07963   2 RKQRGFTLIELLVALAILAILLAAAL 27
PRK10574 PRK10574
putative major pilin subunit; Provisional
 5-26 9.99e-08

putative major pilin subunit; Provisional


Pssm-ID: 236718 [Multi-domain]  Cd Length: 146  Bit Score: 48.11  E-value: 9.99e-08
                         10        20
                 ....*....|....*....|..
gi 647497609   5 RQQGFTLIELMVVLVIIGIASA 26
Cdd:PRK10574   3 KQRGFTLIELMVVIAIIAILSA 24
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 6-29 4.58e-07

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 43.83  E-value: 4.58e-07
                          10        20
                  ....*....|....*....|....
gi 647497609    6 QQGFTLIELMVVLVIIGIASAAIS 29
Cdd:TIGR02532   1 QRGFTLIELLVVLAILGILALIAL 24
ComGC COG4537
Competence protein ComGC [Mobilome: prophages, transposons];
4-23 3.07e-06

Competence protein ComGC [Mobilome: prophages, transposons];


Pssm-ID: 443603 [Multi-domain]  Cd Length: 108  Bit Score: 43.38  E-value: 3.07e-06
                         10        20
                 ....*....|....*....|
gi 647497609   4 SRQQGFTLIELMVVLVIIGI 23
Cdd:COG4537    9 KKEKGFTLIEMLIVLLIISI 28
PilW COG4966
Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];
3-29 1.80e-05

Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];


Pssm-ID: 443992 [Multi-domain]  Cd Length: 158  Bit Score: 42.47  E-value: 1.80e-05
                         10        20
                 ....*....|....*....|....*..
gi 647497609   3 PSRQQGFTLIELMVVLVIIGIASAAIS 29
Cdd:COG4966    1 RRRQRGFTLVELMVALAIGLIVLAAVL 27
PRK10506 PRK10506
prepilin peptidase-dependent protein;
5-26 3.51e-05

prepilin peptidase-dependent protein;


Pssm-ID: 236704 [Multi-domain]  Cd Length: 162  Bit Score: 41.52  E-value: 3.51e-05
                         10        20
                 ....*....|....*....|..
gi 647497609   5 RQQGFTLIELMVVLVIIGIASA 26
Cdd:PRK10506   7 KQRGYTLIELLVVMTIVSILSA 28
PulJ COG4795
Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and ...
 1-89 9.25e-05

Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443823 [Multi-domain]  Cd Length: 118  Bit Score: 39.61  E-value: 9.25e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 647497609   1 MTPSRQQGFTLIELMVVL----VIIGIASAAISLSIRPDplQGLRKDAERLAQLLQVAQAEARvDGRPISWRADAKGFAF 76
Cdd:COG4795    3 RARRRQRGFTLLELLVALaifaLLLLAAYRGLDSVLRSR--ERLEQQAERLQELQRALALLER-DLRQAGPRPDEGGDPE 79

                         90
                 ....*....|...
gi 647497609  77 SRPSDDGRGLDQF 89
Cdd:COG4795   80 PALSGGGSCLIDL 92
pilin_ComGC NF040999
competence type IV pilus major pilin ComGC; ComGC, encoded in the comG operon, is the major ...
 6-23 1.83e-04

competence type IV pilus major pilin ComGC; ComGC, encoded in the comG operon, is the major pilin of a type IV pilus involved in natural transformation of monoderm bacteria (those lacking an outer membrane) such as Bacillus subtilis and Streptococcus pneumoniae. In the seed alignment, Bacillus proteins have a pair of Cys residues likely to form a disulfide bond while Streptococcus proteins lack Cys residues.


Pssm-ID: 468929  Cd Length: 84  Bit Score: 38.26  E-value: 1.83e-04
                         10
                 ....*....|....*...
gi 647497609   6 QQGFTLIELMVVLVIIGI 23
Cdd:NF040999   1 VKGFTLIEMLIVLLIISV 18
typeII_sec_gspG TIGR01710
type II secretion system protein G; This model represents GspG, protein G of the main terminal ...
 7-56 4.66e-04

type II secretion system protein G; This model represents GspG, protein G of the main terminal branch of the general secretion pathway, also called type II secretion. It transports folded proteins across the bacterial outer membrane and is widely distributed in Gram-negative pathogens. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


Pssm-ID: 130771 [Multi-domain]  Cd Length: 134  Bit Score: 38.18  E-value: 4.66e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 647497609    7 QGFTLIELMVVLVIIGIASAAISLSIRPDPLQGLRKDAErlAQLLQVAQA 56
Cdd:TIGR01710   1 RGFTLLEIMVVLVILGLLAALVAPKLFSQADKAKAQVAK--AQIKALKNA 48
PilV COG4967
Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];
1-55 5.59e-04

Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];


Pssm-ID: 443993 [Multi-domain]  Cd Length: 86  Bit Score: 36.89  E-value: 5.59e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 647497609   1 MTPSRQQGFTLIELMVVLVIIGIA-SAAISLSIRpdpLQGLRKDAERLAQLLQVAQ 55
Cdd:COG4967    5 RRRRRQRGFTLIEVLVALVILSIGlLGLAGLQAA---SLRSSQDARQRTQAALLAQ 57
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH