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Conserved domains on  [gi|648541780|ref|WP_026233531|]
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MULTISPECIES: IS66 family insertion sequence element accessory protein TnpB [Bradyrhizobium]

Protein Classification

transposase( domain architecture ID 10529723)

transposase binds to the end of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism

Gene Ontology:  GO:0004803|GO:0003677|GO:0006313
PubMed:  30454069|11418571|20215432

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TnpB_IS66 pfam05717
IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The ...
 13-107 1.64e-52

IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The function of these proteins is uncertain, but they are probably essential for transposition.


:

Pssm-ID: 461723  Cd Length: 96  Bit Score: 159.95  E-value: 1.64e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 648541780   13 ATKPVDFRKGMEGLAILVREHMQADPFSGAVYVFRAKRADRIKLIFWDGTGLCLFAKRLEDGVFRWPK-IEDGVMRLSAA 91
Cdd:pfam05717   1 ATGPTDMRKGIDGLAALVQEALGLDPFSGHLFVFRNRRRDRIKILYWDGDGFWLYYKRLERGRFVWPRrAGDGVVELTAA 80

                          90
                  ....*....|....*.
gi 648541780   92 ELSALLEGLDWRRVHA 107
Cdd:pfam05717  81 QLSWLLEGIDWRRPRA 96
 
Name Accession Description Interval E-value
TnpB_IS66 pfam05717
IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The ...
 13-107 1.64e-52

IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The function of these proteins is uncertain, but they are probably essential for transposition.


Pssm-ID: 461723  Cd Length: 96  Bit Score: 159.95  E-value: 1.64e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 648541780   13 ATKPVDFRKGMEGLAILVREHMQADPFSGAVYVFRAKRADRIKLIFWDGTGLCLFAKRLEDGVFRWPK-IEDGVMRLSAA 91
Cdd:pfam05717   1 ATGPTDMRKGIDGLAALVQEALGLDPFSGHLFVFRNRRRDRIKILYWDGDGFWLYYKRLERGRFVWPRrAGDGVVELTAA 80

                          90
                  ....*....|....*.
gi 648541780   92 ELSALLEGLDWRRVHA 107
Cdd:pfam05717  81 QLSWLLEGIDWRRPRA 96
IS66_TnpB NF033819
IS66 family insertion sequence element accessory protein TnpB; The IS66 family insertion ...
 10-99 9.69e-49

IS66 family insertion sequence element accessory protein TnpB; The IS66 family insertion sequence element encodes a DDE transposase TnpC, and two accessory proteins, TnpA and TnpB. It has been assumed that the TnpA, TnpB, and TnpC proteins are produced independently in appropriate amounts and form a complex, which acts as a transposase to promote the transposition of an IS66 family element.


Pssm-ID: 411392  Cd Length: 90  Bit Score: 150.33  E-value: 9.69e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 648541780  10 VMVATKPVDFRKGMEGLAILVREHMQADPFSGAVYVFRAKRADRIKLIFWDGTGLCLFAKRLEDGVFRWPKIEDGVMRLS 89
Cdd:NF033819   1 IYLATGPTDMRKGIDGLAALVQEVLGLDPFSGHLFVFRNRRRDRLKILYWDGSGFCLYYKRLEKGRFRWPRAADGVVALT 80

                         90
                 ....*....|
gi 648541780  90 AAELSALLEG 99
Cdd:NF033819  81 PAQLAWLLEG 90
COG3436 COG3436
Transposase [Mobilome: prophages, transposons];
4-76 7.78e-05

Transposase [Mobilome: prophages, transposons];


Pssm-ID: 442662 [Multi-domain]  Cd Length: 416  Bit Score: 40.40  E-value: 7.78e-05
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 648541780   4 PSGTVRVMVATKPVDFRK--GMEGLAILVREHMQADPfsGAVYVFRAKRaDRIKLIFWDGTGLC---LFAKRLEDGVF 76
Cdd:COG3436   71 PEHLPREEVLHEPTDMRKidGLAAIGEEVSEELDLDP--GALFVFRNRR-DKYKCLYCDGTGLVyapAPAKRLEKGLA 145
 
Name Accession Description Interval E-value
TnpB_IS66 pfam05717
IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The ...
 13-107 1.64e-52

IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The function of these proteins is uncertain, but they are probably essential for transposition.


Pssm-ID: 461723  Cd Length: 96  Bit Score: 159.95  E-value: 1.64e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 648541780   13 ATKPVDFRKGMEGLAILVREHMQADPFSGAVYVFRAKRADRIKLIFWDGTGLCLFAKRLEDGVFRWPK-IEDGVMRLSAA 91
Cdd:pfam05717   1 ATGPTDMRKGIDGLAALVQEALGLDPFSGHLFVFRNRRRDRIKILYWDGDGFWLYYKRLERGRFVWPRrAGDGVVELTAA 80

                          90
                  ....*....|....*.
gi 648541780   92 ELSALLEGLDWRRVHA 107
Cdd:pfam05717  81 QLSWLLEGIDWRRPRA 96
IS66_TnpB NF033819
IS66 family insertion sequence element accessory protein TnpB; The IS66 family insertion ...
 10-99 9.69e-49

IS66 family insertion sequence element accessory protein TnpB; The IS66 family insertion sequence element encodes a DDE transposase TnpC, and two accessory proteins, TnpA and TnpB. It has been assumed that the TnpA, TnpB, and TnpC proteins are produced independently in appropriate amounts and form a complex, which acts as a transposase to promote the transposition of an IS66 family element.


Pssm-ID: 411392  Cd Length: 90  Bit Score: 150.33  E-value: 9.69e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 648541780  10 VMVATKPVDFRKGMEGLAILVREHMQADPFSGAVYVFRAKRADRIKLIFWDGTGLCLFAKRLEDGVFRWPKIEDGVMRLS 89
Cdd:NF033819   1 IYLATGPTDMRKGIDGLAALVQEVLGLDPFSGHLFVFRNRRRDRLKILYWDGSGFCLYYKRLEKGRFRWPRAADGVVALT 80

                         90
                 ....*....|
gi 648541780  90 AAELSALLEG 99
Cdd:NF033819  81 PAQLAWLLEG 90
COG3436 COG3436
Transposase [Mobilome: prophages, transposons];
4-76 7.78e-05

Transposase [Mobilome: prophages, transposons];


Pssm-ID: 442662 [Multi-domain]  Cd Length: 416  Bit Score: 40.40  E-value: 7.78e-05
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 648541780   4 PSGTVRVMVATKPVDFRK--GMEGLAILVREHMQADPfsGAVYVFRAKRaDRIKLIFWDGTGLC---LFAKRLEDGVF 76
Cdd:COG3436   71 PEHLPREEVLHEPTDMRKidGLAAIGEEVSEELDLDP--GALFVFRNRR-DKYKCLYCDGTGLVyapAPAKRLEKGLA 145
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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