Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase (DMB-PRT), also called CobT; Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase (DMB-PRT/CobT, not to be confused with the CobT subunit of cobaltochelatase, which does not belong to this group) catalyzes the synthesis of alpha-ribazole-5'-phosphate, from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB). This function is essential to the anaerobic biosynthesis pathway of cobalamin (vitamin B12), which is the largest and most complex cofactor in a number of enzyme-catalyzed reactions in bacteria, archaea and eukaryotes. Only eubacteria and archaebacteria can synthesize vitamin B12; multicellular organisms have lost this ability during evolution. DMB-PRT/CobT works sequentially with CobC (a phosphatase) to couple the lower ligand of cobalamin to a ribosyl moiety. DMB is the most common lower ligand of cobamides; other lower ligands include adenine, 5-methoxybenzimidazole or phenol. It has been suggested that earlier metabolic or enzymatic steps may control which lower ligand is available to DMB-PRT/CobT. In Salmonella enterica, for example, the lower ligand is DMB under aerobic conditions and adenine or 2-methyladenine under anaerobic conditions. Salmonella enterica DMB-PRT/CobT is a homodimer with two active sites, each active site is comprised of residues from both monomers. This group includes two distinct subfamilies, one archaeal-like, the other comprised of bacterial sequences.

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gi 648655542 111 ADnVEILWLGtPPASIASLPALRPLAPSPAL--RAALpgvdDPAGLPVYVIDPnGFVIMRHAPGSDLGG 177
Cdd:PRK04447  53 AD-AELLLYG-KPLSLPPLPVTPTGCPTPALitRAVL----ELLGIPVLVVDA-GLYVKPKVPYIDLGG 114

hypothetical protein; Provisional

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                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 648655542 111 ADnVEILWLGtPPASIASLPALRPLAPSPAL--RAALpgvdDPAGLPVYVIDPnGFVIMRHAPGSDLGG 177
Cdd:PRK04447  53 AD-AELLLYG-KPLSLPPLPVTPTGCPTPALitRAVL----ELLGIPVLVVDA-GLYVKPKVPYIDLGG 114

hypothetical protein; Provisional

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 648655542 111 ADnVEILWLGtPPASIASLPALRPLAPSPAL--RAALpgvdDPAGLPVYVIDPnGFVIMRHAPGSDLGG 177
Cdd:PRK04447  53 AD-AELLLYG-KPLSLPPLPVTPTGCPTPALitRAVL----ELLGIPVLVVDA-GLYVKPKVPYIDLGG 114