NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|653153389|ref|WP_027402407|]
View 

MULTISPECIES: type II toxin-antitoxin system VapC family toxin [Aphanizomenonaceae]

Protein Classification

type II toxin-antitoxin system VapC family toxin( domain architecture ID 13036944)

type II toxin-antitoxin (TA) system VapC family toxin functions as a ribonuclease that is neutralized by its cognate antitoxin VapB

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
PIN_VapC4-5_FitB-like cd18744
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-131 2.38e-51

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


:

Pssm-ID: 350311  Cd Length: 128  Bit Score: 158.81  E-value: 2.38e-51
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKITISESKEEKIFISCITYFEIRRGFLAVDAPKQRARFEEFCQEYPIIFLDDlAILEKAAQ 83
Cdd:cd18744    1 LLDTDILSALLKGNPKVVEKFREYLKEYGKITISIITYYEILRGLLAKNATKQLEVFEEFCSNNEVLPLDD-ESADIAAE 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 653153389  84 IHANLRLRGVPIQTEDVLIAATAILKDLIVVSND-SDLDRVEGLSLENW 131
Cdd:cd18744   80 IYADLRKKGTLIDDADILIAATAIANNLVLVTNNtKHFQRIEGLKLENW 128
 
Name Accession Description Interval E-value
PIN_VapC4-5_FitB-like cd18744
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-131 2.38e-51

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350311  Cd Length: 128  Bit Score: 158.81  E-value: 2.38e-51
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKITISESKEEKIFISCITYFEIRRGFLAVDAPKQRARFEEFCQEYPIIFLDDlAILEKAAQ 83
Cdd:cd18744    1 LLDTDILSALLKGNPKVVEKFREYLKEYGKITISIITYYEILRGLLAKNATKQLEVFEEFCSNNEVLPLDD-ESADIAAE 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 653153389  84 IHANLRLRGVPIQTEDVLIAATAILKDLIVVSND-SDLDRVEGLSLENW 131
Cdd:cd18744   80 IYADLRKKGTLIDDADILIAATAIANNLVLVTNNtKHFQRIEGLKLENW 128
VapC COG1487
Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];
3-131 4.26e-33

Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];


Pssm-ID: 441096  Cd Length: 128  Bit Score: 112.65  E-value: 4.26e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   3 YLLDTNIVSLALKQNSQILHKItISESKEEKIFISCITYFEIRRGFLAVDAPKQRARFEEFCQEYPIIFLDDlAILEKAA 82
Cdd:COG1487    1 YLLDTNVLIDLLRGRPPAVLAW-LAELEAGELAISAITLAELLYGARRSPDAKRRAALEAFLEAFPVLPFDA-EAARRAG 78

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 653153389  83 QIHANLRLRGVPIQTEDVLIAATAILKDLIVVSND-SDLDRVEGLSLENW 131
Cdd:COG1487   79 ELRAALRRAGRPIGLADLLIAATALAHGLTLVTRNtKDFERIPGLKVEPW 128
PRK13725 PRK13725
tRNA(fMet)-specific endonuclease VapC;
1-131 6.94e-11

tRNA(fMet)-specific endonuclease VapC;


Pssm-ID: 184277  Cd Length: 132  Bit Score: 55.80  E-value: 6.94e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   1 MNYLLDTNIVSLALKQN-SQILHKITISESKeekIFISCITYFEIRRGFLAVDAPKQR-ARFEEFCQEYPIIFLDDLAIL 78
Cdd:PRK13725   2 LKFMLDTNICIFTIKNKpEHVRERFNLNTGR---MCISSVTLMELIYGAEKSQMPERNlAVIEGFVSRLEVLDYDTAAAT 78

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 653153389  79 EkAAQIHANLRLRGVPIQTEDVLIAATAILKDLIVVSNDS-DLDRVEGLSLENW 131
Cdd:PRK13725  79 H-TGQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTrEFERVPGIRIEDW 131
PIN pfam01850
PIN domain;
3-126 1.54e-07

PIN domain;


Pssm-ID: 426475  Cd Length: 121  Bit Score: 46.63  E-value: 1.54e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389    3 YLLDTNI-VSLALKQNSQILHKITISesKEEKIFISCITYFEIRRGFLAV-DAPKQRARFEEFCQEYPIIFLD-DLAILE 79
Cdd:pfam01850   1 IVLDTSVlIALLRGEPLHEAARELLE--AAGELVTSAIVLAELLYGLNRRlGLGKAAELVELLLLLSALEVLPiDAELAE 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 653153389   80 KAAQIHANLRLRGVPiqteDVLIAATAILKDLIVVSNDSDLDRVEGL 126
Cdd:pfam01850  79 EAAELRLKYGKLGPN----DALIAATAKEHGAKLITFDEDFARVAKL 121
 
Name Accession Description Interval E-value
PIN_VapC4-5_FitB-like cd18744
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-131 2.38e-51

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350311  Cd Length: 128  Bit Score: 158.81  E-value: 2.38e-51
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKITISESKEEKIFISCITYFEIRRGFLAVDAPKQRARFEEFCQEYPIIFLDDlAILEKAAQ 83
Cdd:cd18744    1 LLDTDILSALLKGNPKVVEKFREYLKEYGKITISIITYYEILRGLLAKNATKQLEVFEEFCSNNEVLPLDD-ESADIAAE 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 653153389  84 IHANLRLRGVPIQTEDVLIAATAILKDLIVVSND-SDLDRVEGLSLENW 131
Cdd:cd18744   80 IYADLRKKGTLIDDADILIAATAIANNLVLVTNNtKHFQRIEGLKLENW 128
VapC COG1487
Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];
3-131 4.26e-33

Ribonuclease/mRNA interferase VapC, contains PIN domain [Defense mechanisms];


Pssm-ID: 441096  Cd Length: 128  Bit Score: 112.65  E-value: 4.26e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   3 YLLDTNIVSLALKQNSQILHKItISESKEEKIFISCITYFEIRRGFLAVDAPKQRARFEEFCQEYPIIFLDDlAILEKAA 82
Cdd:COG1487    1 YLLDTNVLIDLLRGRPPAVLAW-LAELEAGELAISAITLAELLYGARRSPDAKRRAALEAFLEAFPVLPFDA-EAARRAG 78

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 653153389  83 QIHANLRLRGVPIQTEDVLIAATAILKDLIVVSND-SDLDRVEGLSLENW 131
Cdd:COG1487   79 ELRAALRRAGRPIGLADLLIAATALAHGLTLVTRNtKDFERIPGLKVEPW 128
PIN_VapC4-5_FitB-like cd09881
VapC-like PIN domain of Mycobacterium tuberculosis VapC4 and VapC5, and Neisseria gonorrhoeae ...
 4-130 3.03e-29

VapC-like PIN domain of Mycobacterium tuberculosis VapC4 and VapC5, and Neisseria gonorrhoeae FitB and related proteins; This family includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This family belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350229  Cd Length: 127  Bit Score: 102.94  E-value: 3.03e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKItISESKEEKIFISCITYFEIRRG-FLAVDAPKQRARFEEFCQEYPIIFLDDlAILEKAA 82
Cdd:cd09881    1 LLDTNICIDLLRGRPPSVLER-LEELLPEDLAISSITLAELLYGaEKSQRPEKNLAALEAFLSRLEVLPFDE-EAAEIYG 78

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 653153389  83 QIHANLRLRGVPIQTEDVLIAATAILKDLIVVSNDS-DLDRVEGLSLEN 130
Cdd:cd09881   79 EIRAELEKKGKPIGDNDLLIAATALAHGLTLVTNNTkHFERIPGLKVEN 127
PIN_VapC4-5_FitB-like cd18745
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 3-131 8.54e-24

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350312  Cd Length: 130  Bit Score: 89.09  E-value: 8.54e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   3 YLLDTNIVSLALKQNSQILHKITISESKEEkIFISCITYFEIRRGFLA-VDAPKQRARFEEFCQEYPIIFLDDLAIlEKA 81
Cdd:cd18745    1 YLLDTNTVIALFRGRGPVLRARLRAAAPSD-LALSSIVLYELEYGAAKsARPERNRARLEELLSLIEVLPFDEEDA-RAA 78

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 653153389  82 AQIHANLRLRGVPIQTEDVLIAATAILKDLIVVS-NDSDLDRVEGLSLENW 131
Cdd:cd18745   79 AEIRADLERAGTPIGPYDTLIAGQALARGATLVThNVREFSRVPGLKVEDW 129
PIN_VapC4-5_FitB-like cd18742
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-131 7.70e-21

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350309  Cd Length: 127  Bit Score: 81.43  E-value: 7.70e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKItiSESKEEKIFISCITYFEIRRG-FLAVDAPKQRARFEEFCQEYPIIFLDDlAILEKAA 82
Cdd:cd18742    1 LLDTDICIYWLKGNESVKEKF--LEVGNEPLSISVITYAELYYGaYNSQRVEKNLARVERFKELLPVLPLSE-AIAETFG 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 653153389  83 QIHANLRLRGVPIQTEDVLIAATAILKDLIVVSNDSD-LDRVEGLSLENW 131
Cdd:cd18742   78 KIKADLRKKGNPIDDFDLLIASTALANNLILVTNNTKhFKRIPGLKIENW 127
PIN_VapC4-5_FitB-like cd18750
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-130 2.28e-19

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350317  Cd Length: 127  Bit Score: 77.67  E-value: 2.28e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKI-TISESKeekIFISCITYFEIRRGfLAVDAPKQRARfeefcqeYPII--FLDDLAIL-- 78
Cdd:cd18750    1 LLDTCTISDFVRGNPNTLERLkSTSPSL---IAISSITVMEIEYG-LRLNPQRAKKL-------RPILeaFLSSITILpf 69

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 653153389  79 -----EKAAQIHANLRLRGVPIQTEDVLIAATAILKDLIVV-SNDSDLDRVEGLSLEN 130
Cdd:cd18750   70 deedaIAAAAIRAALKKQGTPIGAYDVLIAGTALAHGLILVtSNTKEFQRVDGLVIEN 127
PIN_VapC4-5_FitB-like cd18743
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-132 3.99e-19

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350310  Cd Length: 127  Bit Score: 76.79  E-value: 3.99e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKItiSESKEEKIFISCITYFEIRRGFLAVDAP-KQRARFEEFCQEYPIIFLDDlaILEKAA 82
Cdd:cd18743    1 LLDTNICIFLLRGKYGVAEKI--EEVGLENCFISEITIAELLYGAENSARPeKNRKLVDDFLSLFTVLPISD--ALEEYA 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 653153389  83 QIHANLRLRGVPIQTEDVLIAATAILKDLIVVS-NDSDLDRVEGLSLENWV 132
Cdd:cd18743   77 KEKARLRRQGKLIDDFDLLIGATAIANNLTLVTeNVKHFARIPGIKLENWI 127
PIN_VapC4-5_FitB-like cd18748
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-131 1.27e-18

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350315  Cd Length: 128  Bit Score: 75.65  E-value: 1.27e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKItISESKEEKIFISCITYFEIRRGFLAVDAPKQRARFEEFCQEYPIIFLDDLAIlEKAAQ 83
Cdd:cd18748    1 LLDTNIISDLIRNPRGKVARR-IREVGEDAVCTSIIVASELRFGAAKKGSERLARRVEAVLSRIPVLPLEPPAD-EHYAR 78

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 653153389  84 IHANLRLRGVPIQTEDVLIAATAILKDLIVVSNDS-DLDRVEGLSLENW 131
Cdd:cd18748   79 IRADLERQGTPIGPNDLLIAAHALALDLTLVTDNTrEFSRVPGLKVENW 127
PIN_VapC4-5_FitB-like cd18747
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-131 2.40e-18

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350314  Cd Length: 132  Bit Score: 75.27  E-value: 2.40e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQ--NSQILHKItisESKEEKIFISCITYFEIRRGFLAVDAPKQRARFEEFCQeypiIFLDDLAIL--- 78
Cdd:cd18747    1 LLDTNILSEPLKPepNANVLARL---ERHEGEIAIAAVVWHELLYGCYRLPEGKRKRILEYYLE----DVVSGLPILpyd 73

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 653153389  79 EKAAQIHANLRLR----GVPIQTEDVLIAATAILKDLIVVS-NDSDLDRVEGLSLENW 131
Cdd:cd18747   74 EAAAEWHAEERARltaiGKTPPFADGQIAAIAVANNLILVTrNTKDFQGFEGLRLENW 131
PIN_VapC4-5_FitB-like cd18740
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-130 7.28e-18

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350307  Cd Length: 126  Bit Score: 73.75  E-value: 7.28e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKiTISESKEEKIFISCITYFEIRRGfLAV--DAPKQRARFEEFcqeypiifLDDLAIL--- 78
Cdd:cd18740    1 MLDTNTVSHIIKGRPPAVRE-RLRAVPMASICISSITEAELLYG-LAKrpEAKRLHAAVREF--------LLRVDVLpwd 70

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 653153389  79 EKAAQIHANLR----LRGVPIQTEDVLIAATAILKDLIVVSNDSDLDRVEGLSLEN 130
Cdd:cd18740   71 SSVARTYGDLRaaleAQGKTLGPLDMMIAAHALALGATLVTNDRAFRRVPGLAVED 126
PIN_VapC4-5_FitB-like cd18753
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-128 7.89e-18

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350320  Cd Length: 123  Bit Score: 73.66  E-value: 7.89e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKItiseSKEEKIFISCITYFEIRRGFLA-VDAPKQRARFEEFCQEYPIIFLD-DLAILEKA 81
Cdd:cd18753    1 LLDTNAYSALLRGDPAVLEAL----ARADEIYLPVIVLGELLFGFRKgSREEENRERLEEFLSSPRVEVLPiDEETAEIY 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 653153389  82 AQIHANLRLRGVPIQTEDVLIAATAILKDLIVVSNDSDLDRVEGLSL 128
Cdd:cd18753   77 AEIKSALKKKGTPIPENDIWIAALALEHGLPLVTRDAHFDKIPGLKL 123
PIN_VapC4-5_FitB-like cd18751
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-131 8.60e-17

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350318  Cd Length: 133  Bit Score: 71.23  E-value: 8.60e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQ-NSQILHKITISESKEEKIFISCITYFEIRRGFLAVDAPKQRARFEEFCQEYPIIFLDdLAILEKAA 82
Cdd:cd18751    1 LLDTDILSEILKGkNPQVVARARAYLAAFGRYTISVITVMEVVRGLQKVQRAERLQQFLEMIESAEVLPLD-RTAAELAG 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 653153389  83 QIHANLRLRGVPIQTEDVLIAATAILKDLIVVS-NDSDLDRVEG----LSLENW 131
Cdd:cd18751   80 RIYADLERTGQPIGRADPMIAAIALRHGLVLVTgNTAHYQRIQElgypLVLENW 133
PIN_VapC4-5_FitB-like cd18752
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-131 3.96e-16

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350319  Cd Length: 130  Bit Score: 69.20  E-value: 3.96e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQN-SQILHKITISESKEEKiFISCITYFEIRRGflAVDAPKQRARFEEFcQEYPIIFLDDLAILEKAA 82
Cdd:cd18752    1 LFDTDAISELLKKRpSPPLLERLNSIPREDQ-FTSAITVGELYYG--AYRSDRRLRLLEEL-LEPVISRVTILPFDRESA 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 653153389  83 QIHANLR----LRGVPIQTEDVLIAATAILKDLIVVS-NDSDLDRVEGLSLENW 131
Cdd:cd18752   77 RVYGRLRaeleKKGTPLGEADLQIAAIALAHNLTLVTgNIRHFERIPGLRVENW 130
PIN_VapC4-5_FitB-like cd18746
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-130 1.96e-15

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350313 [Multi-domain]  Cd Length: 133  Bit Score: 67.46  E-value: 1.96e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKITISESKEEKIFISCITYFEIRRGF--LAVDAPKQRARFEEFCQEYPIIFLD-----DLA 76
Cdd:cd18746    1 LLDTNVISELRKPRPDPGVVAWLAAVDEDDLFLSVITIGEIRKGIerLRRRDPARAARLEAWLDGLLLRFAGrilpvDAE 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 653153389  77 ILEKAAQIHANLRLrgvPIQTEDVLIAATAILKDLIVVS-NDSDLDRVeGLSLEN 130
Cdd:cd18746   81 VARRWGRLHAPAPA---PLPVADALIAATALVHGLTLVTrNVRDFEGT-GVPLLN 131
PIN_VapC4-5_FitB-like cd18754
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-129 4.41e-15

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350321  Cd Length: 128  Bit Score: 66.47  E-value: 4.41e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKITISESKEEKIFISCITYFEIRRGFLAVDAPKQ-RARFEEFCQEYPIIFLDDlAILEKAA 82
Cdd:cd18754    1 ILDTSFLIDLMRGDEAAIEKARELESESVPQRVSSPTVFELYVGVGLSAAPEEeRERIESVLESKPILPLDG-ESARKAG 79

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 653153389  83 QIHANLRLRGVPIQTEDVLIAATAILKDLIVVS-NDSDLDRVEGLSLE 129
Cdd:cd18754   80 RIDGQLRREGEPIDPGDAMIAATALVHDEPVLTrNVKHFERIDGLEVE 127
PIN_HiVapC1-like cd18735
VapC-like PIN domain of Haemophilus influenzae VapC1 and related proteins; Haemophilus ...
 4-131 6.49e-15

VapC-like PIN domain of Haemophilus influenzae VapC1 and related proteins; Haemophilus influenzae VapC1 has endonucleolytic activity with RNA, it cleaves initiator tRNA between the anticodon stem and loop, but does not cleave mRNA, rRNA or tmRNA, and has no activity on ssDNA or dsDNA. This subfamily belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. FitB is a toxin of the FitAB TA system. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350302  Cd Length: 128  Bit Score: 66.08  E-value: 6.49e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNS-QILHKITISESKEekIFISCITYFEIRRG-FLAVDAPKQRARFEEFCQEYPIIFLDDLAIlEKA 81
Cdd:cd18735    1 LLDTNICIYIRKQRPpSVLERFAQLQPGE--VAMSVITYGELLYGaEKSQRRAKALEALERLAELIPVLPLPEEVA-EHY 77

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 653153389  82 AQIHANLRLRGVPIQTEDVLIAATAILKDLIVVSND-SDLDRVEGLSLENW 131
Cdd:cd18735   78 GEIRADLEKKGTPIGNNDLWIAAHALALGLTLVTNNtREFQRVPGLKVENW 128
PIN_VapC4-5_FitB-like cd18741
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-129 4.40e-14

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350308  Cd Length: 120  Bit Score: 63.67  E-value: 4.40e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKItiSESKEEKIFISCITYFEIRRGflaVDAPKQRARFEEFCQEYPIIFLDDlAILEKAAQ 83
Cdd:cd18741    1 LLDTDILIDFLRGNPSAAEKL--LELLGGNLAISVITVAELYAG---ARNKKELEKLEKLLSLFEVIPVDE-EIAELAGE 74

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 653153389  84 IHANLRLRgVPIQTEDVLIAATAILKDLIVVS-NDSDLDRVEGLSLE 129
Cdd:cd18741   75 LRRKYRKS-HGLGLPDALIAATALEHGLTLVTlNVKHFPMIEGLKLP 120
PIN_CcVapC1-like cd18736
VapC-like PIN domain of Caulobacter Crescentus VapC1-like and related proteins; This subfamily ...
 4-131 3.47e-13

VapC-like PIN domain of Caulobacter Crescentus VapC1-like and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Caulobacter Crescentus VapC1, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. This subfamily belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. FitB is a toxin of the FitAB TA system. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350303  Cd Length: 123  Bit Score: 61.43  E-value: 3.47e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNI-VSLALKQNSQILHKItiSESKEEKIFISCITYFEIRRGfLAVDAPKQRARFEEFCQEYPIIFLDDLAIlEKAA 82
Cdd:cd18736    1 LLDTNIcIYLLKGQPPALARRV--AACEPGEVVISAITLAELAYG-VRRSAPPRRAALDALLEVIPVLPFDEAAA-AAYA 76

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 653153389  83 QIHANLR-LRGVpiqtEDVLIAATAILKDLIVVSND-SDLDRVEGLSLENW 131
Cdd:cd18736   77 RIRAAFPfRRAS----FDRLIAAHALALGLTLVTNNeADFADIPGLRVENW 123
PIN_VapC4-5_FitB-like cd18738
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-130 4.55e-12

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350305  Cd Length: 118  Bit Score: 58.31  E-value: 4.55e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQilHKITISESKEEKIFISCITYFEirrgFLAVD--APKQRARFEEFCQEYPIIFLDDlAILEKA 81
Cdd:cd18738    1 LLDTNIIIYYLKGELP--ESLEELLEELNEPAISVITKIE----LLGWPklTPEEEEEIEDFLSLAEVIPLDD-EIARKT 73

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 653153389  82 AQIHANLRlrgvpIQTEDVLIAATAILKDLIVVS-NDSDLDRVEGLSLEN 130
Cdd:cd18738   74 IELRKQYK-----IKLPDAIIAATALVHGLTLVTrNVKDFKKIPGLKLLN 118
PIN_VapC4-5_FitB-like cd18739
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 31-130 4.86e-12

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350306  Cd Length: 124  Bit Score: 58.50  E-value: 4.86e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389  31 EEKIFISCITYFEIRRGFLAVDAPKQRARFEEFCQEYPIIFLDDLAILEkAAQIHANLRLRGVPIQTEDVLIAATAILKD 110
Cdd:cd18739   26 DEPLVTPTIALYEVYVGAVRSAGETDLDGVREDLDWVEPLDFDEEVARE-AAQIQAELLEAGEPINPPDLMIAATARSLG 104

                         90       100
                 ....*....|....*....|
gi 653153389 111 LIVVSNDSDLDRVEGLSLEN 130
Cdd:cd18739  105 ATLVTRDGDFERIDGLEVEN 124
PIN_RfVapC1-like cd18733
VapC-like PIN domain of Rickettsia felis VapC1 and related proteins; This subfamily includes ...
 4-130 6.51e-12

VapC-like PIN domain of Rickettsia felis VapC1 and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Rickettsia felis VapC1, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350300  Cd Length: 131  Bit Score: 58.38  E-value: 6.51e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKItiSESKEEKIFISCITYFEIRRGFLAVDAPKQRARFEEFCQ----EYPIIFLDdLAILE 79
Cdd:cd18733    3 IIDTSVFIAAERGRLDLEALQ--QERPDEDVYISAITASELLHGVHRAKTPERRARREAFVEaileAFPVLDFD-LEVAR 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 653153389  80 KAAQIHANLRLRGVPIQTEDVLIAATAILKDL-IVVSNDSDLDRVEGLSLEN 130
Cdd:cd18733   80 THARLWAELAKRGITIGAHDLLIAATALAHGYsVLTANVRDFKRIPGLEVEE 131
PIN_MtVapC3-like_start cd09882
VapC-like PIN domain of Mycobacterium tuberculosis VapC3 toxin and related proteins; The ...
 31-130 7.16e-12

VapC-like PIN domain of Mycobacterium tuberculosis VapC3 toxin and related proteins; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, and VapC21. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350230  Cd Length: 128  Bit Score: 58.37  E-value: 7.16e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389  31 EEKIFISCITYFEIRRGFLAvdaPKQRARFEEFCQEYPIIFLDDLaILEKAAQIHANLRLRGV-PIQTEDVLIAATAILK 109
Cdd:cd09882   29 GEDVALTGIILTEVLQGIRD---EKEFQRVKEYLLALPILPLTGE-TYIAAARLYRRLRRKGVtVRSTIDCLIAATAIEN 104

                         90       100
                 ....*....|....*....|....
gi 653153389 110 DLIVVSNDSDLD---RVEGLSLEN 130
Cdd:cd09882  105 GLTLLHNDRDFEaiaKHTGLKLYR 128
PRK13725 PRK13725
tRNA(fMet)-specific endonuclease VapC;
1-131 6.94e-11

tRNA(fMet)-specific endonuclease VapC;


Pssm-ID: 184277  Cd Length: 132  Bit Score: 55.80  E-value: 6.94e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   1 MNYLLDTNIVSLALKQN-SQILHKITISESKeekIFISCITYFEIRRGFLAVDAPKQR-ARFEEFCQEYPIIFLDDLAIL 78
Cdd:PRK13725   2 LKFMLDTNICIFTIKNKpEHVRERFNLNTGR---MCISSVTLMELIYGAEKSQMPERNlAVIEGFVSRLEVLDYDTAAAT 78

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 653153389  79 EkAAQIHANLRLRGVPIQTEDVLIAATAILKDLIVVSNDS-DLDRVEGLSLENW 131
Cdd:PRK13725  79 H-TGQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTrEFERVPGIRIEDW 131
PIN_VapC4-5_FitB-like cd18749
uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; ...
 4-128 4.87e-10

uncharacterized subgroup of the PIN_VapC4-5_FitB-like subfamily of the PIN domain superfamily; The PIN_VapC4-5_FitB-like subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 ribonuclease toxins of the VapBC toxin/antitoxin (TA) system, and Neisseria gonorrhoeae FitB toxin of the FitAB TA system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350316  Cd Length: 126  Bit Score: 53.40  E-value: 4.87e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKITISESKEekIFISCITYFEIRRGFLAVD-APKQRARFEEFCQEYPIIFLDDlAILEKAA 82
Cdd:cd18749    1 VLDTDVASYLFKGRLLAALYAPRLVGKI--LVISFVTVAELYFWAEKRGwGEKRLQQLEAWLSRYVVLPYDE-EIARTWG 77

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 653153389  83 QIHANLRLRGVPIQTEDVLIAATAILKDLIVVS-NDSDLDRVEGLSL 128
Cdd:cd18749   78 RLRAEAQRRGRPRPTNDAWIAATALTHGLPLAThNVKDFEDFPGLQL 124
PIN_MtVapC4-C5-like cd18768
VapC-like PIN domain of Mycobacterium tuberculosis VapC4, VapC5, and related proteins; This ...
 4-126 1.72e-09

VapC-like PIN domain of Mycobacterium tuberculosis VapC4, VapC5, and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 toxin of the VapBC toxin/antitoxin (TA) system. This family belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. M. tuberculosis VapC4 interacts with, and cleaves tRNA44Cys-GCA. M. tuberculosis VapC5 has endonucleolytic activity with RNA, this activity is low with dsRNA, and no activity has been demonstrated on dsDNA. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350335  Cd Length: 123  Bit Score: 52.03  E-value: 1.72e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNiVSLALKQNSQILhkitiSESKEEKIFISCITYFEIRRGFLAVDAPKQRAR---FEEFCQEYPIIFLDDLAilek 80
Cdd:cd18768    1 LADTS-VFIAQESGRALD-----GDLLPDEIAVSVVTLAELRAGVLAARDLATRARrlaTLQLAADLEPLPIDDAV---- 70

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 653153389  81 aAQIHANLRLR----GVPIQTEDVLIAATAILKDLIVVSNDSDLDRVEGL 126
Cdd:cd18768   71 -AAAWARLRLElresGRRMRVNDSWIAATALAHDLPVVTQDADFDALPDL 119
PIN_RfVapC2-like cd18734
VapC-like PIN domain of Rickettsia felis VapC2 and related proteins; This subfamily includes ...
 4-131 2.47e-09

VapC-like PIN domain of Rickettsia felis VapC2 and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Rickettsia felis VapC2, a ribonuclease toxin of the VapBC toxin/antitoxin (TA) system. Rickettsia felis VapC2 cleaves single-stranded RNA. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC TA systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350301  Cd Length: 129  Bit Score: 51.73  E-value: 2.47e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHKITISESKEEKIFISCITYFEIRRGFlavdapkQRARFEEFCQEYPIIFLDDLAIL---EK 80
Cdd:cd18734    1 MLDTNICVYLIKKKPDSYYARLEKLQKNNDIAISSIVLAELQYGV-------SKSQYKEQNQAALNMFLMKLEVLpfeEK 73

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 653153389  81 AAQIHANLRL----RGVPIQTEDVLIAATAILKDLIVVSNDS-DLDRVEGLSLENW 131
Cdd:cd18734   74 ATFYYGEIRAelekSGQVIGGNDLLIAAHALAENATLVTNNIkEFARVPNLILENW 129
VapC COG1848
VapC family ribonuclease, toxin component of the VapBC toxin-antitoxin module, contains PIN ...
 3-126 2.73e-09

VapC family ribonuclease, toxin component of the VapBC toxin-antitoxin module, contains PIN domain [Defense mechanisms];


Pssm-ID: 441453  Cd Length: 134  Bit Score: 51.52  E-value: 2.73e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   3 YLLDTNIVSLALKQNS-------QILHKItisESKEEKIFISCITYFEIRRGFL---AVDAPKQRARFEEFCQEYPIIFL 72
Cdd:COG1848    2 ILLDTNVLIYALEGDSpfherarELLERA---EEGEEELYTSPLVLAEVLYVLTrpgGLSLEEARELLEALLSNIEVVPV 78

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 653153389  73 DDlAILEKAAQIHANLRLRgvpiqTEDVLIAATAILKDL-IVVSNDSDLDRVEGL 126
Cdd:COG1848   79 DE-EILEEAAELLAKYGLR-----LNDALHLATALEHGAdALVTFDRDFARVPGL 127
PIN_PH0500-like cd18730
VapC-like PIN-domain of Pyrococcus horikoshii protein PH0500 and related proteins; This ...
 15-118 3.40e-08

VapC-like PIN-domain of Pyrococcus horikoshii protein PH0500 and related proteins; This subfamily includes Pyrococcus horikoshii protein PH0500, a protein with possible exonuclease activity and involvement in DNA or RNA editing. This subfamily belongs to the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350297  Cd Length: 126  Bit Score: 48.47  E-value: 3.40e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389  15 KQNSQILhKITISESKeekIFISCITYFE--IRRGFLAVDAPKQRARFEEFcqeYPIIFLDDlAILEKAAQIHANLRLRG 92
Cdd:cd18730   14 KQNKRLL-EFTLAKFE---VFVPIISVYAylLTKAYLGKNPEEELKILKEI---YNIVPLDD-EIIVKAAEIEASLLKKG 85

                         90       100
                 ....*....|....*....|....*.
gi 653153389  93 VPIQTEDVLIAATAILKDLIVVSNDS 118
Cdd:cd18730   86 VFLDLEDLLTAVTAIVTNSLLVTDDP 111
PIN_VapC-FitB-like cd09875
VapC-like PIN domain of ribonucleases (toxins), VapC and FitB, of prokaryotic toxin/antitoxin ...
 4-130 1.23e-07

VapC-like PIN domain of ribonucleases (toxins), VapC and FitB, of prokaryotic toxin/antitoxin operons, Pyrococcus horikoshii protein PH0500, and other similar bacterial and archaeal homologs; PIN (PilT N terminus) domain-containing proteins of prokaryotic toxin/antitoxin (TA) operons, such as, Mycobacterium tuberculosis VapC of the VapBC (virulence associated proteins) TA operon, and Neisseria gonorrhoeae FitB of the FitAB (fast intracellular trafficking) TA operon, as well as, the archaeal Pyrococcus horikoshii protein PH0500 are included in this family. Toxins of TA operons are believed to be involved in growth inhibition by regulating translation and are nearly always co-expressed with an antitoxin, a cognate protein inhibitor, forming an inert protein complex. Disassociation of the complex activates the ribonuclease activity of the toxin. In N. gonorrhoeae, FitA and FitB form a heterodimer: FitA is the DNA binding subunit and FitB contains a ribonuclease activity that is blocked by the presence of FitA. A tetramer of FitAB heterodimers binds DNA from the fitAB upstream promoter region with high affinity. This results in both sequestration of FitAB and repression of fitAB transcription. It is thought that FitAB release from the DNA and subsequent dissociation both slows N. gonorrhoeae replication and transcytosis by an as yet undefined mechanism. The toxin M. tuberculosis VapC is a structural homolog of N. gonorrhoeae FitB, but their antitoxin partners, VapB and FitA, respectively, differ structurally. The M. tuberculosis VapC-5 is proposed to be both an endoribonuclease and an exoribonuclease that can act on free RNA in a similar manner to the endo and exonuclease Flap endonuclease-1 (FEN1). VapC-like toxins are structural homologs of FEN1-like PIN domains, but lack the extensive arch/clamp region and the H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region, seen in FEN1-like PIN domains. PIN domains within this group typically contain three or four conserved acidic residues that cluster at the C-terminal end of the beta-sheet and form a negatively charged pocket near the center of the molecule. These putative active site residues are thought to bind Mg2+ and/or Mn2+ ions and be essential for single-stranded ribonuclease activity. VapC-like PIN domains are single domain proteins that form dimers and dimerization configures the active sites in a groove along the long-axis of the structure.


Pssm-ID: 350223  Cd Length: 130  Bit Score: 47.18  E-value: 1.23e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNiVSLALKQNSQILHKITISESKE-EKIFISCITYFEIR----RGFLAVDAPKQRARFEEFCQEYPIIFLDDlAIL 78
Cdd:cd09875    1 LLDTS-VLIWLLDNDPKSHLRSLADRIErGKVYVSIVSRWELAigvnINKLRLNYAFADLENLLADLAIPVLPLTP-RIA 78

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 653153389  79 EKAAQIHANLRLRGVPIQTEDVLIAATAILKDLIVVSNDSDLDRVEGLSLEN 130
Cdd:cd09875   79 DEYLEACRNLPLRGRHRSPFDRMIAAQALQHGFTVAHRDEDFKAINAVNLEW 130
PIN pfam01850
PIN domain;
3-126 1.54e-07

PIN domain;


Pssm-ID: 426475  Cd Length: 121  Bit Score: 46.63  E-value: 1.54e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389    3 YLLDTNI-VSLALKQNSQILHKITISesKEEKIFISCITYFEIRRGFLAV-DAPKQRARFEEFCQEYPIIFLD-DLAILE 79
Cdd:pfam01850   1 IVLDTSVlIALLRGEPLHEAARELLE--AAGELVTSAIVLAELLYGLNRRlGLGKAAELVELLLLLSALEVLPiDAELAE 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 653153389   80 KAAQIHANLRLRGVPiqteDVLIAATAILKDLIVVSNDSDLDRVEGL 126
Cdd:pfam01850  79 EAAELRLKYGKLGPN----DALIAATAKEHGAKLITFDEDFARVAKL 121
PIN_NgFitB-like cd18731
VapC-like PIN domain of Neisseria gonorrhoeae FitB and related proteins; This subfamily ...
 3-114 2.93e-07

VapC-like PIN domain of Neisseria gonorrhoeae FitB and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Neisseria gonorrhoeae FitB toxin of the FitAB toxin/antitoxin (TA) system. This subfamily belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. In N. gonorrhoeae, FitA and FitB form a heterodimer: FitA is the DNA binding subunit and FitB contains a ribonuclease activity that is blocked by the presence of FitA. A tetramer of FitAB heterodimers binds DNA from the fitAB upstream promoter region with high affinity. This results in both sequestration of FitAB and repression of fitAB transcription. It is thought that FitAB release from the DNA and subsequent dissociation both slows N. gonorrhoeae replication and transcytosis by an as yet undefined mechanism. The toxin M. tuberculosis VapC is a structural homolog of N. gonorrhoeae FitB, but their antitoxin partners, VapB and FitA, respectively, differ structurally. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350298  Cd Length: 136  Bit Score: 46.26  E-value: 2.93e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   3 YLLDTNIVSLALKQ--NSQILHkiTISESKEEKIFISCITYFEIRRGfLAVDAPKQR-----ARFEEFCQEY---PIIFL 72
Cdd:cd18731    1 IVLDTNVVSELMRPapDPAVLA--WLDAQPAEDLYLTAITVAELLYG-IALLPDGRRrdaleAAAEALLAEDfagRILPF 77

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 653153389  73 DDLAILEkAAQIHANLRLRGVPIQTEDVLIAATAILKDLIVV 114
Cdd:cd18731   78 DAAAAEA-YAEIVAARRRAGRPISVADAQIAAIARAHGATLA 118
PIN_MtVapC3-like cd18766
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 4-123 4.74e-07

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350333  Cd Length: 130  Bit Score: 45.50  E-value: 4.74e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVSLALKQNSQILHK-----ITISEsKEEKIFISCITYFEIRRGFlavDAPKQRARFEEFCQEYPIIFLDDLAIL 78
Cdd:cd18766    1 LVDTSVWSLALRRPKHAVNReatllKQIIE-KGEPIALTGIILQELLQGI---RNDKLFDQLKEHLEAFPLILPTREDHI 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 653153389  79 EkAAQIHANLRLRGVPIQTEDVLIAATAILKDLIVVSNDSDLDRV 123
Cdd:cd18766   77 E-AARLRNDCRRKGVQVSAIDALIAQMCISHDLTLFTTDKDFKHI 120
COG3744 COG3744
PIN domain nuclease, a component of toxin-antitoxin system (PIN domain) [Defense mechanisms];
1-122 1.26e-06

PIN domain nuclease, a component of toxin-antitoxin system (PIN domain) [Defense mechanisms];


Pssm-ID: 442958  Cd Length: 128  Bit Score: 44.44  E-value: 1.26e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   1 MNYLLDTNIVSLALKQNSQILHKI-TISESKEEKIFISCITYFEI----RRGFLAVDAPkQRARFEEFCQEYPIIFLD-D 74
Cdd:COG3744    1 MKLLLDTHALLWWLLGDERLSPAArELIEDPANELYVSAASLWEIaikvRLGKLDLPED-LDEWLEELLEELGFELLPiT 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 653153389  75 LAILEKAAQIhanlrlrgvPIQTEDV---LIAATAILKDLIVVSNDSDLDR 122
Cdd:COG3744   80 PEHALAAAQL---------PLHHRDPfdrLLIAQALVEGLPLVTADRKIAA 121
PIN_MtVapC3-like cd18763
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 29-124 3.56e-06

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350330  Cd Length: 128  Bit Score: 43.42  E-value: 3.56e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389  29 SKEEKIFISCITYFEIRRGflaVDAPKQ----RARFEEFcqeypiIFLD-DLAILEKAAQIHANLRLRGVPIQ-TEDVLI 102
Cdd:cd18763   27 EQREDLCICGVILTEVLQG---IRDDKEyaktKELLESL------LFLPmTQSTFIRAADIYRTLRKKGITIRkPVDCMI 97

                         90       100
                 ....*....|....*....|..
gi 653153389 103 AATAILKDLIVVSNDSDLDRVE 124
Cdd:cd18763   98 AAVALEHDVSLLHNDRDFDPIA 119
PIN_MtVapC3-like cd18762
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 71-123 7.69e-05

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350329  Cd Length: 130  Bit Score: 39.49  E-value: 7.69e-05
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 653153389  71 FLDDLAILEKAAQIHANLRLRGVPIQ-TEDVLIAATAILKDLIVVSNDSDLDRV 123
Cdd:cd18762   67 LKDGIESYEEAAKIYFRCRRKGITIRsTIDCLIAQTAIENNLYLLHNDRDFDNI 120
PIN_MtVapC3-like cd18764
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 68-131 2.47e-04

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350331  Cd Length: 129  Bit Score: 38.33  E-value: 2.47e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 653153389  68 PIIFLDDLAILEKAAQIHANLRLRGVPI-QTEDVLIAATAILKDLIVVSNDSDLDRV-EGLSLENW 131
Cdd:cd18764   63 PILELDPYEAAIGAADLYRSLRKKGVTIrKSNDCLIAHYAIKFDLPLVHNDRDFDLIaKYSKLKVY 128
PIN_MtVapC3-like cd18759
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 31-128 2.55e-04

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350326  Cd Length: 126  Bit Score: 38.34  E-value: 2.55e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389  31 EEKIFISCITYFEIRRGflaVDAPKQRARFEEFCQEYPIIFLDDlAILEKAAQIHANLRLRGVPIQTEDVLIAATAILKD 110
Cdd:cd18759   28 EDEVCTCGIVLAELLQG---AKSEKELSLLEDFLSALPYLPITD-ELWIKAGELSFSLRKKGITIPLSDLYIAALAIENN 103

                         90       100
                 ....*....|....*....|.
gi 653153389 111 LIVVSNDSDLDRVE---GLSL 128
Cdd:cd18759  104 LELFTLDKHFEIIPkhtGLKL 124
PIN_MtVapC15-VapC11-like cd18756
VapC-like PIN domain of Mycobacterium tuberculosis VapC11, VapC15, and related proteins; This ...
 54-123 2.93e-04

VapC-like PIN domain of Mycobacterium tuberculosis VapC11, VapC15, and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC11 and VapC15 toxins. M. tuberculosis VapC11 and VapC15 cleave tRNA3 Leu-CAG, VapC11 may additionally cleave tRNA13Leu-GAG and tRNA10Gln-CTG. This subgroup belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350323  Cd Length: 129  Bit Score: 37.98  E-value: 2.93e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 653153389  54 PKQRARFEEFCQEYPIIFLDDLAILEKAAQIHANLRLRGVPIQ-TEDVLIAATAILKDLIVVSNDSDLDRV 123
Cdd:cd18756   49 EAHLARLRRLLNGLRLLPLDPALDFEAAAAIYRACRRGGHTVRsLIDCLIAAVAIRHGATLLHRDRDFDVI 119
PIN_MtVapC3-like cd18761
uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; ...
 81-123 2.81e-03

uncharacterized subgroup of the VapC3-like nuclease subfamily of the PIN domain superfamily; The VapC3-like nuclease subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of various Mycobacterium tuberculosis VapC toxins including VapC3, VapC11, VapC15, VapC21, VapC25, VapC28, VapC29, VapC30, VapC32, VapC33, VapC37, and VapC39. It belongs to the VapC-like family of the PIN domain nuclease superfamily. VapC is a PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350328  Cd Length: 127  Bit Score: 35.42  E-value: 2.81e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 653153389  81 AAQIHANLRLRGVPIQTE-DVLIAATAILKDLIVVSNDSDLDRV 123
Cdd:cd18761   74 AARIYFDLRRQGLTVRSPiDCCIAQLALENDLTLLHDDRDFETI 117
PIN_MtVapC4-C5_like cd18732
VapC-like PIN domain of Mycobacterium tuberculosis VapC4, VapC5, and related proteins; This ...
 4-126 3.23e-03

VapC-like PIN domain of Mycobacterium tuberculosis VapC4, VapC5, and related proteins; This subfamily includes the Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis VapC4 and VapC5 toxin of the VapBC toxin/antitoxin (TA) system. This family belongs to the PIN_VapC4-5_FitB-like subfamily of the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. M. tuberculosis VapC4 interacts with, and cleaves tRNA44Cys-GCA. M. tuberculosis VapC5 has endonucleolytic activity with RNA, this activity is low with dsRNA, and no activity has been demonstrated on dsDNA. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350299  Cd Length: 124  Bit Score: 35.32  E-value: 3.23e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 653153389   4 LLDTNIVslalkqnsqILHKITISESKEEKIFISCITYFEIRRGFLAVDAPKQRA----RFEEFCQEYPIIFLDDLAilE 79
Cdd:cd18732    1 LLDTSVV---------IDLERGDPADLPDEVAISAITLAELAFGVLAADTRAERAqrlqRLLQAEDFDPLPFDAEAA--R 69

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 653153389  80 KAAQIHANLRLRGVPIQ--TEDVLIAATAILKDL-IVVSNDSDLDRVEGL 126
Cdd:cd18732   70 AYGRLAAAVRAAGRKPRprANDLLIAATAIAHGLpLLTRNPDDFAGLDGL 119
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH