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Conserved domains on  [gi|655281837|ref|WP_028691235|]
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MULTISPECIES: polynucleotide adenylyltransferase PcnB [Pseudomonas]

Protein Classification

polynucleotide adenylyltransferase PcnB( domain architecture ID 11493326)

polynucleotide adenylyltransferase PcnB, or poly(A) polymerase, creates the 3' poly(A) tail found in some mRNAs

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
pcnB TIGR01942
poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as ...
 25-449 0e+00

poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as plasmid copy number protein). These enzymes sequentially add adenosine nucleotides to the 3' end of RNAs, targeting them for degradation by the cell. This was originally described for anti-sense RNAs, but was later demonstrated for mRNAs as well. Members of this family are as yet limited to the gamma- and beta-proteobacteria, with putative members in the Chlamydiacae and spirochetes. This family has homology to tRNA nucleotidyltransferase (cca).


:

Pssm-ID: 130997 [Multi-domain]  Cd Length: 410  Bit Score: 638.38  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837   25 VINKSQHSLQRSQFSRHAVSIVERLQAAGYQAYLVGGCVRDLLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVH 104
Cdd:TIGR01942   1 VIPESEHNIPRQSFSAHALNVVERLKGAGYQAYIVGGAVRDLLLGIEPKDFDVVTSATPEEVRKLFRNSRIVGRRFRLVH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  105 VHFGRDIIEVATFRAphsdedqsdSHRSSHNASGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRN 184
Cdd:TIGR01942  81 VSFGRQIIEVATFRS---------GHKSSVNAEGRILKDNVYGTLEEDAWRRDFTVNALYYDPSREVIIDYVGGMEDLKN 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  185 RLLRLIGDPTHRYQEDPVRMLRAVRFAAKLDFGIEKHTVQPIRELAPMLRDIPPARLFEESLKLFLNGQGAITFEMLVDL 264
Cdd:TIGR01942 152 RRLRLIGDPRSRYQEDPVRMLRALRFSVKLEFTIDESTARPIRESAPLLKGIPPARLFEEILKLLFSGRSAALFRMLCGY 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  265 QLFEPLFPASSQALEERPTYTHTLISQALSN-TDLRVKQGKPVTPAFLFAALLWPALPGRALYLQSQGVPPIPAMNGAAH 343
Cdd:TIGR01942 232 QLLEPLFPSVAYALRESPKFESAFTVQALVNdTDFRVKRDKPVTPAFLYAALLWPGLVFRVADAPDQGVMPYPAVFDAIR 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  344 DLIAEQCARIAIPKRFTLPIREIWDMQERLPRRSGKRADMLLDNPRFRAGYDFLLLRESAGEETDGLGEWWTDYQDANDS 423
Cdd:TIGR01942 312 DFVEEQCAPISIPKRFSIPTREIWQMQLRLERRSGMRARKLLGHARFRAAYDLLLLREVCGRENAVLGEWWTPYQLASEE 391

                         410       420
                  ....*....|....*....|....*.
gi 655281837  424 QRRDMIRelgSRDDgaggTAGPRKRK 449
Cdd:TIGR01942 392 ERTEMTR---SAAR----HEGNRGRA 410
 
Name Accession Description Interval E-value
pcnB TIGR01942
poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as ...
 25-449 0e+00

poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as plasmid copy number protein). These enzymes sequentially add adenosine nucleotides to the 3' end of RNAs, targeting them for degradation by the cell. This was originally described for anti-sense RNAs, but was later demonstrated for mRNAs as well. Members of this family are as yet limited to the gamma- and beta-proteobacteria, with putative members in the Chlamydiacae and spirochetes. This family has homology to tRNA nucleotidyltransferase (cca).


Pssm-ID: 130997 [Multi-domain]  Cd Length: 410  Bit Score: 638.38  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837   25 VINKSQHSLQRSQFSRHAVSIVERLQAAGYQAYLVGGCVRDLLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVH 104
Cdd:TIGR01942   1 VIPESEHNIPRQSFSAHALNVVERLKGAGYQAYIVGGAVRDLLLGIEPKDFDVVTSATPEEVRKLFRNSRIVGRRFRLVH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  105 VHFGRDIIEVATFRAphsdedqsdSHRSSHNASGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRN 184
Cdd:TIGR01942  81 VSFGRQIIEVATFRS---------GHKSSVNAEGRILKDNVYGTLEEDAWRRDFTVNALYYDPSREVIIDYVGGMEDLKN 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  185 RLLRLIGDPTHRYQEDPVRMLRAVRFAAKLDFGIEKHTVQPIRELAPMLRDIPPARLFEESLKLFLNGQGAITFEMLVDL 264
Cdd:TIGR01942 152 RRLRLIGDPRSRYQEDPVRMLRALRFSVKLEFTIDESTARPIRESAPLLKGIPPARLFEEILKLLFSGRSAALFRMLCGY 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  265 QLFEPLFPASSQALEERPTYTHTLISQALSN-TDLRVKQGKPVTPAFLFAALLWPALPGRALYLQSQGVPPIPAMNGAAH 343
Cdd:TIGR01942 232 QLLEPLFPSVAYALRESPKFESAFTVQALVNdTDFRVKRDKPVTPAFLYAALLWPGLVFRVADAPDQGVMPYPAVFDAIR 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  344 DLIAEQCARIAIPKRFTLPIREIWDMQERLPRRSGKRADMLLDNPRFRAGYDFLLLRESAGEETDGLGEWWTDYQDANDS 423
Cdd:TIGR01942 312 DFVEEQCAPISIPKRFSIPTREIWQMQLRLERRSGMRARKLLGHARFRAAYDLLLLREVCGRENAVLGEWWTPYQLASEE 391

                         410       420
                  ....*....|....*....|....*.
gi 655281837  424 QRRDMIRelgSRDDgaggTAGPRKRK 449
Cdd:TIGR01942 392 ERTEMTR---SAAR----HEGNRGRA 410
pcnB PRK11623
poly(A) polymerase I; Provisional
 25-457 8.19e-161

poly(A) polymerase I; Provisional


Pssm-ID: 236939 [Multi-domain]  Cd Length: 472  Bit Score: 463.07  E-value: 8.19e-161
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  25 VINKSQHSLQRSQFSRHAVSIVERLQAAGYQAYLVGGCVRDLLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVH 104
Cdd:PRK11623  38 VIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAH 117

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 105 VHFGRDIIEVATFRAPHsDEDQSDSHRSSHNASGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRN 184
Cdd:PRK11623 118 VMFGPEIIEVATFRGHH-EGNESDRNTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKE 196

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 185 RLLRLIGDPTHRYQEDPVRMLRAVRFAAKLDFGIEKHTVQPIRELAPMLRDIPPARLFEESLKLFLNGQGAITFEMLVDL 264
Cdd:PRK11623 197 GVIRLIGNPETRYREDPVRMLRAVRFAAKLDMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY 276

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 265 QLFEPLFPASSQALEER-PTYTHTLISQALSNTDLRVKQGKPVTPAFLFAALLWPALPGRALYL-QSQGVPPIPAMNGAA 342
Cdd:PRK11623 277 HLFQPLFPTITRYFTENgDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLEHAQKIaQESGLAYYDAFALAM 356

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 343 HDLIAEQCARIAIPKRFTLPIREIWDMQERLPRRSGKRADMLLDNPRFRAGYDFLLLRESA--GEETDGLGEWWTDYQDA 420
Cdd:PRK11623 357 NDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAYDLLALRAEVenNAELQRLAQWWGEFQVS 436

                        410       420       430
                 ....*....|....*....|....*....|....*..
gi 655281837 421 NDSQRRDMIRELGsrDDGAGGTAGPRKRKRSGGKRKR 457
Cdd:PRK11623 437 APPQQKGMLNELD--DDPAPRRRTRRPRKRAPRREGT 471
PcnB COG0617
tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and ...
 37-420 6.72e-118

tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and biogenesis]; tRNA nucleotidyltransferase/poly(A) polymerase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440382 [Multi-domain]  Cd Length: 391  Bit Score: 350.65  E-value: 6.72e-118
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  37 QFSRHAVSIVERLQAAGYQAYLVGGCVRDLLLGITPKDFDVATSATPEQVRAEFR---NARIIGRRFKLVHVHFGRDIIE 113
Cdd:COG0617    1 RLSPNALKVLEALEEAGFEAYLVGGAVRDLLLGRPPKDIDIVTVATPEEVAALFRkalRTVPVGRDFGTVTVVFGGEKIE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 114 VATFRAPHSDEDqsdshrsshnasGRiLRDNVYG-TLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRNRLLRLIGD 192
Cdd:COG0617   81 VATARTERYYGD------------GR-RPFVEFGdTLEEDLARRDFTINALAYDLNDGELIDPFGGLADLEARVIRTVGD 147

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 193 PTHRYQEDPVRMLRAVRFAAKLDFGIEKHTVQPIRELAPMLRDIPPARLFEESLKLFLNGQGAITFEMLVDLQLFEPLfp 272
Cdd:COG0617  148 PEERFREDPLRILRAVRFAARLGFTIEPETLAAIREMAGLLDRLSAERVWDELLKLLLSPHPSRGLELLRETGLLEVL-- 225

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 273 assqaleerptythtlisqalsntdlrvkqgkpvtpAFLFAALLWPALPGRAlylQSQGVPPIPAMNGAAHDLIAEQCAR 352
Cdd:COG0617  226 ------------------------------------ALRLAALLHDLGKPAT---REDGLPTFHGHEEAGAELAEALLKR 266

                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 353 IAIPKRFTLPIREIWDMQERLPRRSGKRADMLLD--NPRFRAGYDFLLLREsAGEETDGLGEWWTDYQDA 420
Cdd:COG0617  267 LRLPNRERKLVRELVELHLRFHGLGELRDSAVRRllERGPEALEDLLLLRE-NGLEYPELQERLAELLEA 335
PolyA_pol pfam01743
Poly A polymerase head domain; This family includes nucleic acid independent RNA polymerases, ...
 56-188 3.74e-51

Poly A polymerase head domain; This family includes nucleic acid independent RNA polymerases, such as Poly(A) polymerase, which adds the poly (A) tail to mRNA EC:2.7.7.19. This family also includes the tRNA nucleotidyltransferase that adds the CCA to the 3' of the tRNA EC:2.7.7.25. This family is part of the nucleotidyltransferase superfamily.


Pssm-ID: 396348 [Multi-domain]  Cd Length: 126  Bit Score: 169.38  E-value: 3.74e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837   56 AYLVGGCVRDLLLGITPKDFDVATSATPEQVRAEFRNARII----GRRFKLVHVHFGRDIIEVATFRAPHSDEDQSDSHR 131
Cdd:pfam01743   1 LYIVGGAVRDLLLGKTPKDVDIATDATPEQVATLFRRRRIVhllsGIEFGTIHVIFGNQILEVATFRIEFDESDFRNPRS 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 655281837  132 SSHNasgrilrdnvyGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRNRLLR 188
Cdd:pfam01743  81 EEYT-----------GTLEEDAKRRDFTINALAYNPNSGEVIDYFGGIKDLKSGVIR 126
NT_ClassII-CCAase cd05398
Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the ...
 42-184 1.51e-39

Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)], one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction. This Class II group is comprised mainly of eubacterial and eukaryotic enzymes and includes Bacillus stearothermophilus CCAase, Escherichia coli poly(A) polymerase I, human mitochondrial CCAase, and Saccharomyces cerevisiae CCAase (CCA1). CCA-adding enzymes have a single catalytic pocket, which recognizes both ATP and CTP substrates. Included in this subgroup are CC- and A-adding enzymes from various ancient species of bacteria such as Aquifex aeolicus; these enzymes collaborate to add CCA to tRNAs. This family belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are fairly well conserved in this family. Escherichia coli CCAase is related to this group but has not been included in this alignment as this enzyme lacks the N-terminal helix conserved in the remainder of the NT superfamily.


Pssm-ID: 143388 [Multi-domain]  Cd Length: 139  Bit Score: 139.27  E-value: 1.51e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  42 AVSIVERL-QAAGYQAYLVGGCVRDLLLGITPKDFDVATSA-TPEQVR--AEFRNARIIGR--RFKLVHVHFGRDIIEVA 115
Cdd:cd05398    4 LLKLLRELkKALGYEAYLVGGAVRDLLLGRPPKDIDIATDAdGPEFAEalFKKIGGRVVGLgeEFGTATVVINGLTIDVA 83

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 655281837 116 TFRAphsdEDQSDSHRsshnasgriLRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRN 184
Cdd:cd05398   84 TLRT----ETYTDPGR---------RPPVVGFTIEEDLLRRDFTINAMAYDLDDGELIDPFGGLKDLEN 139
 
Name Accession Description Interval E-value
pcnB TIGR01942
poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as ...
 25-449 0e+00

poly(A) polymerase; This model describes the pcnB family of poly(A) polymerases (also known as plasmid copy number protein). These enzymes sequentially add adenosine nucleotides to the 3' end of RNAs, targeting them for degradation by the cell. This was originally described for anti-sense RNAs, but was later demonstrated for mRNAs as well. Members of this family are as yet limited to the gamma- and beta-proteobacteria, with putative members in the Chlamydiacae and spirochetes. This family has homology to tRNA nucleotidyltransferase (cca).


Pssm-ID: 130997 [Multi-domain]  Cd Length: 410  Bit Score: 638.38  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837   25 VINKSQHSLQRSQFSRHAVSIVERLQAAGYQAYLVGGCVRDLLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVH 104
Cdd:TIGR01942   1 VIPESEHNIPRQSFSAHALNVVERLKGAGYQAYIVGGAVRDLLLGIEPKDFDVVTSATPEEVRKLFRNSRIVGRRFRLVH 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  105 VHFGRDIIEVATFRAphsdedqsdSHRSSHNASGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRN 184
Cdd:TIGR01942  81 VSFGRQIIEVATFRS---------GHKSSVNAEGRILKDNVYGTLEEDAWRRDFTVNALYYDPSREVIIDYVGGMEDLKN 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  185 RLLRLIGDPTHRYQEDPVRMLRAVRFAAKLDFGIEKHTVQPIRELAPMLRDIPPARLFEESLKLFLNGQGAITFEMLVDL 264
Cdd:TIGR01942 152 RRLRLIGDPRSRYQEDPVRMLRALRFSVKLEFTIDESTARPIRESAPLLKGIPPARLFEEILKLLFSGRSAALFRMLCGY 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  265 QLFEPLFPASSQALEERPTYTHTLISQALSN-TDLRVKQGKPVTPAFLFAALLWPALPGRALYLQSQGVPPIPAMNGAAH 343
Cdd:TIGR01942 232 QLLEPLFPSVAYALRESPKFESAFTVQALVNdTDFRVKRDKPVTPAFLYAALLWPGLVFRVADAPDQGVMPYPAVFDAIR 311

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  344 DLIAEQCARIAIPKRFTLPIREIWDMQERLPRRSGKRADMLLDNPRFRAGYDFLLLRESAGEETDGLGEWWTDYQDANDS 423
Cdd:TIGR01942 312 DFVEEQCAPISIPKRFSIPTREIWQMQLRLERRSGMRARKLLGHARFRAAYDLLLLREVCGRENAVLGEWWTPYQLASEE 391

                         410       420
                  ....*....|....*....|....*.
gi 655281837  424 QRRDMIRelgSRDDgaggTAGPRKRK 449
Cdd:TIGR01942 392 ERTEMTR---SAAR----HEGNRGRA 410
pcnB PRK11623
poly(A) polymerase I; Provisional
 25-457 8.19e-161

poly(A) polymerase I; Provisional


Pssm-ID: 236939 [Multi-domain]  Cd Length: 472  Bit Score: 463.07  E-value: 8.19e-161
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  25 VINKSQHSLQRSQFSRHAVSIVERLQAAGYQAYLVGGCVRDLLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVH 104
Cdd:PRK11623  38 VIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAH 117

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 105 VHFGRDIIEVATFRAPHsDEDQSDSHRSSHNASGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRN 184
Cdd:PRK11623 118 VMFGPEIIEVATFRGHH-EGNESDRNTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKE 196

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 185 RLLRLIGDPTHRYQEDPVRMLRAVRFAAKLDFGIEKHTVQPIRELAPMLRDIPPARLFEESLKLFLNGQGAITFEMLVDL 264
Cdd:PRK11623 197 GVIRLIGNPETRYREDPVRMLRAVRFAAKLDMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY 276

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 265 QLFEPLFPASSQALEER-PTYTHTLISQALSNTDLRVKQGKPVTPAFLFAALLWPALPGRALYL-QSQGVPPIPAMNGAA 342
Cdd:PRK11623 277 HLFQPLFPTITRYFTENgDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLEHAQKIaQESGLAYYDAFALAM 356

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 343 HDLIAEQCARIAIPKRFTLPIREIWDMQERLPRRSGKRADMLLDNPRFRAGYDFLLLRESA--GEETDGLGEWWTDYQDA 420
Cdd:PRK11623 357 NDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAYDLLALRAEVenNAELQRLAQWWGEFQVS 436

                        410       420       430
                 ....*....|....*....|....*....|....*..
gi 655281837 421 NDSQRRDMIRELGsrDDGAGGTAGPRKRKRSGGKRKR 457
Cdd:PRK11623 437 APPQQKGMLNELD--DDPAPRRRTRRPRKRAPRREGT 471
PcnB COG0617
tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and ...
 37-420 6.72e-118

tRNA nucleotidyltransferase/poly(A) polymerase [Translation, ribosomal structure and biogenesis]; tRNA nucleotidyltransferase/poly(A) polymerase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440382 [Multi-domain]  Cd Length: 391  Bit Score: 350.65  E-value: 6.72e-118
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  37 QFSRHAVSIVERLQAAGYQAYLVGGCVRDLLLGITPKDFDVATSATPEQVRAEFR---NARIIGRRFKLVHVHFGRDIIE 113
Cdd:COG0617    1 RLSPNALKVLEALEEAGFEAYLVGGAVRDLLLGRPPKDIDIVTVATPEEVAALFRkalRTVPVGRDFGTVTVVFGGEKIE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 114 VATFRAPHSDEDqsdshrsshnasGRiLRDNVYG-TLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRNRLLRLIGD 192
Cdd:COG0617   81 VATARTERYYGD------------GR-RPFVEFGdTLEEDLARRDFTINALAYDLNDGELIDPFGGLADLEARVIRTVGD 147

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 193 PTHRYQEDPVRMLRAVRFAAKLDFGIEKHTVQPIRELAPMLRDIPPARLFEESLKLFLNGQGAITFEMLVDLQLFEPLfp 272
Cdd:COG0617  148 PEERFREDPLRILRAVRFAARLGFTIEPETLAAIREMAGLLDRLSAERVWDELLKLLLSPHPSRGLELLRETGLLEVL-- 225

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 273 assqaleerptythtlisqalsntdlrvkqgkpvtpAFLFAALLWPALPGRAlylQSQGVPPIPAMNGAAHDLIAEQCAR 352
Cdd:COG0617  226 ------------------------------------ALRLAALLHDLGKPAT---REDGLPTFHGHEEAGAELAEALLKR 266

                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 353 IAIPKRFTLPIREIWDMQERLPRRSGKRADMLLD--NPRFRAGYDFLLLREsAGEETDGLGEWWTDYQDA 420
Cdd:COG0617  267 LRLPNRERKLVRELVELHLRFHGLGELRDSAVRRllERGPEALEDLLLLRE-NGLEYPELQERLAELLEA 335
PRK13299 PRK13299
tRNA CCA-pyrophosphorylase; Provisional
 41-284 2.51e-60

tRNA CCA-pyrophosphorylase; Provisional


Pssm-ID: 237339 [Multi-domain]  Cd Length: 394  Bit Score: 201.99  E-value: 2.51e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  41 HAVSIVERLQAAGYQAYLVGGCVRDLLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVHVHFGRDIIEVATFRAp 120
Cdd:PRK13299   8 KALPILEKIKEAGFEAYFVGGSVRDYLLGRPIHDVDIATSAYPEEVKAIFPRTVDVGIEHGTVLVLENGEEYEVTTFRT- 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 121 hsDEDQSDSHRSSHNASGRilrdnvygTLEEDAQRRDFTINALYYDpVSERILDYANGVHDIRNRLLRLIGDPTHRYQED 200
Cdd:PRK13299  87 --ESEYVDYRRPSEVTFVR--------SLEEDLKRRDFTINAIAMD-ENGEIIDLFDGLEDLKNRLIRAVGNAEERFQED 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 201 PVRMLRAVRFAAKLDFGIEKHTVQPIRELAPMLRDIPPARLFEESLKLfLNGQGAIT-FEMLVDLQLFE--PLFPASSQA 277
Cdd:PRK13299 156 ALRMMRAVRFASQLGFDLETETFEAMKTQAPLLEKISVERIFVEFEKL-LLGPFWRKgLKLLIETGLYNylPGLKGKEEN 234


                 ....*..
gi 655281837 278 LEERPTY 284
Cdd:PRK13299 235 LLKLTQL 241
PolyA_pol pfam01743
Poly A polymerase head domain; This family includes nucleic acid independent RNA polymerases, ...
 56-188 3.74e-51

Poly A polymerase head domain; This family includes nucleic acid independent RNA polymerases, such as Poly(A) polymerase, which adds the poly (A) tail to mRNA EC:2.7.7.19. This family also includes the tRNA nucleotidyltransferase that adds the CCA to the 3' of the tRNA EC:2.7.7.25. This family is part of the nucleotidyltransferase superfamily.


Pssm-ID: 396348 [Multi-domain]  Cd Length: 126  Bit Score: 169.38  E-value: 3.74e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837   56 AYLVGGCVRDLLLGITPKDFDVATSATPEQVRAEFRNARII----GRRFKLVHVHFGRDIIEVATFRAPHSDEDQSDSHR 131
Cdd:pfam01743   1 LYIVGGAVRDLLLGKTPKDVDIATDATPEQVATLFRRRRIVhllsGIEFGTIHVIFGNQILEVATFRIEFDESDFRNPRS 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 655281837  132 SSHNasgrilrdnvyGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRNRLLR 188
Cdd:pfam01743  81 EEYT-----------GTLEEDAKRRDFTINALAYNPNSGEVIDYFGGIKDLKSGVIR 126
PolyA_pol_arg_C pfam12626
Polymerase A arginine-rich C-terminus; The C-terminus of polymerase A in E coli is ...
 330-450 4.54e-50

Polymerase A arginine-rich C-terminus; The C-terminus of polymerase A in E coli is arginine-rich and is necessary for full functioning of the enzyme.


Pssm-ID: 463647 [Multi-domain]  Cd Length: 116  Bit Score: 166.22  E-value: 4.54e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  330 QGVPPIPAMNGAAHDLIAEQCARIAIPKRFTLPIREIWDMQERLPRRSGKRADMLLDNPRFRAGYDFLLLRESAGEETDG 409
Cdd:pfam12626   1 QGLPPYDALQLAADEVLDEQCQRVAIPRRFTAPMREIWLLQPRLEKRQGKRPFRLLEHPRFRAAYDFLLLRAESGEVLQE 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 655281837  410 LGEWWTDYQDANDSQRRDMIRELGsrddgaGGTAGPRKRKR 450
Cdd:pfam12626  81 LAEWWTRFQEADEAEREAMIAELG------ADAAKKKRRRR 115
NT_ClassII-CCAase cd05398
Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the ...
 42-184 1.51e-39

Nucleotidyltransferase (NT) domain of ClassII CCA-adding enzymes; CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)], one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction. This Class II group is comprised mainly of eubacterial and eukaryotic enzymes and includes Bacillus stearothermophilus CCAase, Escherichia coli poly(A) polymerase I, human mitochondrial CCAase, and Saccharomyces cerevisiae CCAase (CCA1). CCA-adding enzymes have a single catalytic pocket, which recognizes both ATP and CTP substrates. Included in this subgroup are CC- and A-adding enzymes from various ancient species of bacteria such as Aquifex aeolicus; these enzymes collaborate to add CCA to tRNAs. This family belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are fairly well conserved in this family. Escherichia coli CCAase is related to this group but has not been included in this alignment as this enzyme lacks the N-terminal helix conserved in the remainder of the NT superfamily.


Pssm-ID: 143388 [Multi-domain]  Cd Length: 139  Bit Score: 139.27  E-value: 1.51e-39
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  42 AVSIVERL-QAAGYQAYLVGGCVRDLLLGITPKDFDVATSA-TPEQVR--AEFRNARIIGR--RFKLVHVHFGRDIIEVA 115
Cdd:cd05398    4 LLKLLRELkKALGYEAYLVGGAVRDLLLGRPPKDIDIATDAdGPEFAEalFKKIGGRVVGLgeEFGTATVVINGLTIDVA 83

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 655281837 116 TFRAphsdEDQSDSHRsshnasgriLRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGVHDIRN 184
Cdd:cd05398   84 TLRT----ETYTDPGR---------RPPVVGFTIEEDLLRRDFTINAMAYDLDDGELIDPFGGLKDLEN 139
PRK13296 PRK13296
CCA tRNA nucleotidyltransferase;
57-304 5.21e-24

CCA tRNA nucleotidyltransferase;


Pssm-ID: 106256 [Multi-domain]  Cd Length: 360  Bit Score: 102.76  E-value: 5.21e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  57 YLVGGCVRDLLLGITPKDFD-VATSATPEQvraefrnarIIGRRFKLVHVHFgrdiievATFRAPHSDEDQSDSHRSSHN 135
Cdd:PRK13296   4 YLVGGAVRDMLLGITPKDKDwVVVGATEDE---------MLANGFIKIAANF-------PVFIHPQTKQEYALARSEKKT 67

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 136 ASGR---ILRDNVYGTLEEDAQRRDFTINALYYDPvSERILDYANGVHDIRNRLLRligDPTHRYQEDPVRMLRAVRFAA 212
Cdd:PRK13296  68 ASGYhgfEVNFSKYITLEDDLKRRDLTINSIAIDQ-NNKVIDPFNGQADLQNRILR---HTSIAFIEDPLRVVRLARFKA 143

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 213 KL---DFGIEKHTVQPIRELAPM--LRDIPPARLFEESLKLFLNGQgaITFEMLVDLQLFEPLFPASSQALE-------- 279
Cdd:PRK13296 144 QLsnfNFSIAQEMLALIKELVKTgeLNHLTRERLHIEFVKALNNPK--IFFTTLKELEALKIIFPNISCILPlipnksff 221

                        250       260
                 ....*....|....*....|....*
gi 655281837 280 ERPTYTHTLISQALSNTDLRVKQGK 304
Cdd:PRK13296 222 ENPIYKGSNINEKITLCLLKIPQQQ 246
PRK13298 PRK13298
tRNA CCA-pyrophosphorylase; Provisional
 57-289 1.61e-22

tRNA CCA-pyrophosphorylase; Provisional


Pssm-ID: 237338 [Multi-domain]  Cd Length: 417  Bit Score: 99.42  E-value: 1.61e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  57 YLVGGCVRDLLLGITPKDFD-VATSATPEQvraefrnarIIGRRFKLVhvhfGRDIievATFRAPHSDEDQSDSHRSSHN 135
Cdd:PRK13298   4 YLVGGAVRDSLLNLPVKDKDwVVVGGTPKI---------LLSINFQQV----GKDF---PVFLHPETHEEYALARTERKS 67

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 136 ASGR---ILRDNVYGTLEEDAQRRDFTINALYYDPvSERILDYANGVHDIRNRLLRLIgdpTHRYQEDPVRMLRAVRFAA 212
Cdd:PRK13298  68 GVGYtgfITDTSSDVTLEEDLIRRDLTINAIAQDE-NGNYIDPFQGKKDIQLRLLRHV---SESFIEDPLRVLRVARFAA 143

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 213 K---LDFGIEKHTVQPIRELAPM--LRDIPPARLFEESLKLFLNGQGAITFEMLVDLQLFEPLFPaSSQALEERPTYTHT 287
Cdd:PRK13298 144 LlvhLGFKIAKETMILMCIMVKKheLLYLTPERIWNETEKALKTDNPHVYFQVLYECNALKFLFP-EIDFLYEKPYFLNS 222


                 ..
gi 655281837 288 LI 289
Cdd:PRK13298 223 FF 224
cca PRK10885
multifunctional CCA addition/repair protein;
55-230 2.98e-21

multifunctional CCA addition/repair protein;


Pssm-ID: 182810 [Multi-domain]  Cd Length: 409  Bit Score: 95.31  E-value: 2.98e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  55 QAYLVGGCVRDLLLGITPKDFD-VATSATPEQVRAefrnariigRRFKLVhvhfGRDIievATFRAPHSDEDQsdshrss 133
Cdd:PRK10885   2 KIYLVGGAVRDALLGLPVKDRDwVVVGATPEEMLA---------QGYQQV----GKDF---PVFLHPKTHEEY------- 58

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 134 hnASGRILRDNVYG------------TLEEDAQRRDFTINALYYDPvSERILDYANGVHDIRNRLLRLIGDPthrYQEDP 201
Cdd:PRK10885  59 --ALARTERKSGRGytgftcyaapdvTLEEDLIRRDLTINAMAQDD-DGELIDPYGGQRDLEARLLRHVSPA---FAEDP 132

                        170       180       190
                 ....*....|....*....|....*....|..
gi 655281837 202 VRMLRAVRFAAK---LDFGIEKHTVQPIRELA 230
Cdd:PRK10885 133 LRVLRVARFAARfahLGFRIAPETLALMREMV 164
PRK13297 PRK13297
tRNA CCA-pyrophosphorylase; Provisional
 52-229 1.29e-19

tRNA CCA-pyrophosphorylase; Provisional


Pssm-ID: 139469 [Multi-domain]  Cd Length: 364  Bit Score: 90.05  E-value: 1.29e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837  52 AGYQAYLVGGCVRDLLLGITPKDFD-VATSATPEQVraefrnARiigRRFKLVHVHFgrdiievATFRAPHSDEDQS--- 127
Cdd:PRK13297  10 AGLQVYIVGGAVRDALLGLPAGDRDwVVVGATPEDM------AR---RGFIPVGGDF-------PVFLHPRTKEEYAlar 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 655281837 128 DSHRSSHNASGRILRDNVYGTLEEDAQRRDFTINALYYDPVSErILDYANGVHDIRNRLLRLIGDPthrYQEDPVRMLRA 207
Cdd:PRK13297  74 TERKSGRGYKGFTFYTGADVTLEQDLQRRDLTVNAIARTPQGE-LVDPLDGVADVRARVLRHVGEA---FAEDPVRILRL 149

                        170       180
                 ....*....|....*....|...
gi 655281837 208 VRFAAKL-DFGIEKHTVQPIREL 229
Cdd:PRK13297 150 GRFAARFgDFSIAPETMQLCRRM 172
PolyA_pol_RNAbd pfam12627
Probable RNA and SrmB- binding site of polymerase A; This region encompasses much of the RNA ...
 215-278 2.64e-19

Probable RNA and SrmB- binding site of polymerase A; This region encompasses much of the RNA and SrmB binding motifs on polymerase A.


Pssm-ID: 463648 [Multi-domain]  Cd Length: 64  Bit Score: 81.38  E-value: 2.64e-19
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 655281837  215 DFGIEKHTVQPIRELAPMLRDIPPARLFEESLKLFLNGQGAITFEMLVDLQLFEPLFPASSQAL 278
Cdd:pfam12627   1 GFTIEPETREAIRKLAPLLKKISPERIFEELLKLLLSGHPERGLELLRETGLLEYLFPELAAAL 64
PHA01806 PHA01806
hypothetical protein
 56-89 3.18e-04

hypothetical protein


Pssm-ID: 222838  Cd Length: 200  Bit Score: 41.79  E-value: 3.18e-04
                         10        20        30
                 ....*....|....*....|....*....|....
gi 655281837  56 AYLVGGCVRDLLLGITPKDFDVATsATPEQVRAE 89
Cdd:PHA01806  38 VALAGGAARDLMHGAEPKDIDIAL-YGMDDRQAE 70
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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