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Conserved domains on  [gi|657629111|ref|WP_029439815|]
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MULTISPECIES: stage V sporulation protein AE [Bacillus]

Protein Classification

SpoVA/SpoVAEb family sporulation membrane protein( domain architecture ID 10021534)

SpoVA/SpoVAEb family sporulation membrane protein similar to Bacillus subtilis stage V sporulation protein AEB

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SpoVAEB COG5839
Stage V sporulation protein SpoVAEB, subunit of dipicolinate uptake complex [Cell cycle ...
 1-116 6.55e-56

Stage V sporulation protein SpoVAEB, subunit of dipicolinate uptake complex [Cell cycle control, cell division, chromosome partitioning, Amino acid transport and metabolism];


:

Pssm-ID: 444541  Cd Length: 116  Bit Score: 169.18  E-value: 6.55e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 657629111   1 MDFIYAFLVGGAICVVGQVLLDFAKLTPAHLMATFVVIGAVLDGFGLYDKLIKFAGAGATVPITSFGHSLLHGAMHAAEK 80
Cdd:COG5839    1 MDYLWAFLVGGLICVIGQLLMDLTKLTPAHILVTFVVAGAILGGLGLYDPLVDFAGAGATVPLTGFGYSLAKGAIEEVEK 80

                         90       100       110
                 ....*....|....*....|....*....|....*.
gi 657629111  81 HGYIGIGMGMFSLTSAGISAAILFSFFIALICKPKG 116
Cdd:COG5839   81 HGLLGVFTGGFEATAAGISAAIVFGFLAALIFKPKG 116
 
Name Accession Description Interval E-value
SpoVAEB COG5839
Stage V sporulation protein SpoVAEB, subunit of dipicolinate uptake complex [Cell cycle ...
 1-116 6.55e-56

Stage V sporulation protein SpoVAEB, subunit of dipicolinate uptake complex [Cell cycle control, cell division, chromosome partitioning, Amino acid transport and metabolism];


Pssm-ID: 444541  Cd Length: 116  Bit Score: 169.18  E-value: 6.55e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 657629111   1 MDFIYAFLVGGAICVVGQVLLDFAKLTPAHLMATFVVIGAVLDGFGLYDKLIKFAGAGATVPITSFGHSLLHGAMHAAEK 80
Cdd:COG5839    1 MDYLWAFLVGGLICVIGQLLMDLTKLTPAHILVTFVVAGAILGGLGLYDPLVDFAGAGATVPLTGFGYSLAKGAIEEVEK 80

                         90       100       110
                 ....*....|....*....|....*....|....*.
gi 657629111  81 HGYIGIGMGMFSLTSAGISAAILFSFFIALICKPKG 116
Cdd:COG5839   81 HGLLGVFTGGFEATAAGISAAIVFGFLAALIFKPKG 116
spore_V_AE TIGR02839
stage V sporulation protein AE; This model describes stage V sporulation protein AE, a paralog ...
 3-116 8.10e-53

stage V sporulation protein AE; This model describes stage V sporulation protein AE, a paralog of stage V sporulation protein AC. Both are proteins found to present in a species if and only if that species is one of the Firmicutes capable of endospore formation, as of the time of the publication of the genome of Carboxydothermus hydrogenoformans. Mutants in spoVAE have a stage V sproulation defect. [Cellular processes, Sporulation and germination]


Pssm-ID: 131886  Cd Length: 114  Bit Score: 161.37  E-value: 8.10e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 657629111    3 FIYAFLVGGAICVVGQVLLDFAKLTPAHLMATFVVIGAVLDGFGLYDKLIKFAGAGATVPITSFGHSLLHGAMHAAEKHG 82
Cdd:TIGR02839   1 YILAFLVGGAICVIGQLLLDKTKLTPAHILVTFVTVGAILDGFGLYDKLIEFAGAGATVPLTGFGHSLAHGAIEEVEKKG 80

                          90       100       110
                  ....*....|....*....|....*....|....
gi 657629111   83 YIGIGMGMFSLTSAGISAAILFSFFIALICKPKG 116
Cdd:TIGR02839  81 LIGIFTGGFEGTSAGISAAILFGYLMALIFKPKG 114
SpoVAC_SpoVAEB pfam03862
SpoVAC/SpoVAEB sporulation membrane protein; Members of this family are all transcribed from ...
 2-111 3.40e-41

SpoVAC/SpoVAEB sporulation membrane protein; Members of this family are all transcribed from the spoVA operon. Bacillus and Clostridium are two well studied endospore forming bacteria. Spore formation provides a resistance mechanism in response to extreme or unfavourable environmental conditions such as heat, radiation, and chemical agents or nutrient deprivation. The reverse process termed germination takes place where spores develop into growing cells in response to nutrient availability or stress reduction. Nutrient germinant receptors (GRs) and the SpoVA proteins are important players in the germination process. In B.subtilis the SpoVAC and SpoVAEB, belonging to this domain family, are predicted to be membrane proteins, with two to five membrane spanning. Biophysical and biochemical studies suggest that SpoVAC acts as a mechano-sensitive channel with properties that would allow the release of Ca-DPA (dipicolinic acid) and amino acids during germination of the spore. The release of Ca-DPA is a crucial event during spore germination. When expressed in E.coli SpoVAC provides protection against osmotic downshift. Furthermore, SpoVAC acts as channel that facilitates the efflux down the concentration gradient of osmolytes up to a mass of at least 600 Da. Another conserved SpoVA protein in all spore-forming bacteria is SpoVAEb, which appears to be an integral membrane protein with no known function.


Pssm-ID: 427551  Cd Length: 116  Bit Score: 132.10  E-value: 3.40e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 657629111    2 DFIYAFLVGGAICVVGQVLLDF------AKLTPAHLMATFVVIGAVLDGFGLYDKLIKFAGAGATVPITSFGHSLLHGAM 75
Cdd:pfam03862   1 NYLKAFLVGGLICVIGQILMDLtmkllsEEDAPARILVTLVFLGALLTGLGLYDPLAKFAGAGATVPITGFGNSLASGAI 80

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 657629111   76 HAAEKHGYIGIGMGMFSLTSAGISAAILFSFFIALI 111
Cdd:pfam03862  81 EFKKEGGVLGVGAGGFTIAGPVIVYGIVFSFLAGLI 116
 
Name Accession Description Interval E-value
SpoVAEB COG5839
Stage V sporulation protein SpoVAEB, subunit of dipicolinate uptake complex [Cell cycle ...
 1-116 6.55e-56

Stage V sporulation protein SpoVAEB, subunit of dipicolinate uptake complex [Cell cycle control, cell division, chromosome partitioning, Amino acid transport and metabolism];


Pssm-ID: 444541  Cd Length: 116  Bit Score: 169.18  E-value: 6.55e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 657629111   1 MDFIYAFLVGGAICVVGQVLLDFAKLTPAHLMATFVVIGAVLDGFGLYDKLIKFAGAGATVPITSFGHSLLHGAMHAAEK 80
Cdd:COG5839    1 MDYLWAFLVGGLICVIGQLLMDLTKLTPAHILVTFVVAGAILGGLGLYDPLVDFAGAGATVPLTGFGYSLAKGAIEEVEK 80

                         90       100       110
                 ....*....|....*....|....*....|....*.
gi 657629111  81 HGYIGIGMGMFSLTSAGISAAILFSFFIALICKPKG 116
Cdd:COG5839   81 HGLLGVFTGGFEATAAGISAAIVFGFLAALIFKPKG 116
spore_V_AE TIGR02839
stage V sporulation protein AE; This model describes stage V sporulation protein AE, a paralog ...
 3-116 8.10e-53

stage V sporulation protein AE; This model describes stage V sporulation protein AE, a paralog of stage V sporulation protein AC. Both are proteins found to present in a species if and only if that species is one of the Firmicutes capable of endospore formation, as of the time of the publication of the genome of Carboxydothermus hydrogenoformans. Mutants in spoVAE have a stage V sproulation defect. [Cellular processes, Sporulation and germination]


Pssm-ID: 131886  Cd Length: 114  Bit Score: 161.37  E-value: 8.10e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 657629111    3 FIYAFLVGGAICVVGQVLLDFAKLTPAHLMATFVVIGAVLDGFGLYDKLIKFAGAGATVPITSFGHSLLHGAMHAAEKHG 82
Cdd:TIGR02839   1 YILAFLVGGAICVIGQLLLDKTKLTPAHILVTFVTVGAILDGFGLYDKLIEFAGAGATVPLTGFGHSLAHGAIEEVEKKG 80

                          90       100       110
                  ....*....|....*....|....*....|....
gi 657629111   83 YIGIGMGMFSLTSAGISAAILFSFFIALICKPKG 116
Cdd:TIGR02839  81 LIGIFTGGFEGTSAGISAAILFGYLMALIFKPKG 114
SpoVAC_SpoVAEB pfam03862
SpoVAC/SpoVAEB sporulation membrane protein; Members of this family are all transcribed from ...
 2-111 3.40e-41

SpoVAC/SpoVAEB sporulation membrane protein; Members of this family are all transcribed from the spoVA operon. Bacillus and Clostridium are two well studied endospore forming bacteria. Spore formation provides a resistance mechanism in response to extreme or unfavourable environmental conditions such as heat, radiation, and chemical agents or nutrient deprivation. The reverse process termed germination takes place where spores develop into growing cells in response to nutrient availability or stress reduction. Nutrient germinant receptors (GRs) and the SpoVA proteins are important players in the germination process. In B.subtilis the SpoVAC and SpoVAEB, belonging to this domain family, are predicted to be membrane proteins, with two to five membrane spanning. Biophysical and biochemical studies suggest that SpoVAC acts as a mechano-sensitive channel with properties that would allow the release of Ca-DPA (dipicolinic acid) and amino acids during germination of the spore. The release of Ca-DPA is a crucial event during spore germination. When expressed in E.coli SpoVAC provides protection against osmotic downshift. Furthermore, SpoVAC acts as channel that facilitates the efflux down the concentration gradient of osmolytes up to a mass of at least 600 Da. Another conserved SpoVA protein in all spore-forming bacteria is SpoVAEb, which appears to be an integral membrane protein with no known function.


Pssm-ID: 427551  Cd Length: 116  Bit Score: 132.10  E-value: 3.40e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 657629111    2 DFIYAFLVGGAICVVGQVLLDF------AKLTPAHLMATFVVIGAVLDGFGLYDKLIKFAGAGATVPITSFGHSLLHGAM 75
Cdd:pfam03862   1 NYLKAFLVGGLICVIGQILMDLtmkllsEEDAPARILVTLVFLGALLTGLGLYDPLAKFAGAGATVPITGFGNSLASGAI 80

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 657629111   76 HAAEKHGYIGIGMGMFSLTSAGISAAILFSFFIALI 111
Cdd:pfam03862  81 EFKKEGGVLGVGAGGFTIAGPVIVYGIVFSFLAGLI 116
SpoVAC COG5836
Stage V sporulation protein SpoVAC, subunit of dipicolinate uptake complex [Cell cycle control, ...
 3-111 2.19e-22

Stage V sporulation protein SpoVAC, subunit of dipicolinate uptake complex [Cell cycle control, cell division, chromosome partitioning, Amino acid transport and metabolism];


Pssm-ID: 444538  Cd Length: 149  Bit Score: 85.18  E-value: 2.19e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 657629111   3 FIYAFLVGGAICVVGQVLL------DFAKLTPAHLM-ATFVVIGAVLDGFGLYDKLIKFAGAGATVPITSFGHSLLHGAM 75
Cdd:COG5836   29 CLKAFLVGGLICTIGQLIQnfymrfGLSEKDAGNPTsATLIFLSALLTGLGVYDKIAQFAGAGSIVPITGFANSVVSPAI 108

                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 657629111  76 -HAAEkhGYI-GIGMGMFSLTSAGISAAILFSFFIALI 111
Cdd:COG5836  109 eFKSE--GYVlGVGAKMFKIAGPVIVYGVVSAFVVGLI 144
spore_V_AC TIGR02838
stage V sporulation protein AC; This model describes stage V sporulation protein AC, a paralog ...
 4-111 1.37e-17

stage V sporulation protein AC; This model describes stage V sporulation protein AC, a paralog of stage V sporulation protein AE. Both are proteins found to present in a species if and only if that species is one of the Firmicutes capable of endospore formation, as of the time of the publication of the genome of Carboxydothermus hydrogenoformans. Mutants in spoVAC have a stage V sproulation defect. [Cellular processes, Sporulation and germination]


Pssm-ID: 131885  Cd Length: 141  Bit Score: 72.72  E-value: 1.37e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 657629111    4 IYAFLVGGAICVVGQVLLDF--------AKLTPAHLMATFVVIGAVLDGFGLYDKLIKFAGAGATVPITSFGHSLLHGAM 75
Cdd:TIGR02838  21 VMAFLVGGLICLIGQLISDFylryfqfsEKTAGSPTSATLIFISALLTGLGVYDKIAQFAGAGSIVPITGFANAMASPAL 100

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 657629111   76 HAAEKHGYIGIGMGMFSLTSAGISAAILFSFFIALI 111
Cdd:TIGR02838 101 EHKTEGLVLGVGANMFTLAGPVIVYGVSTAYIVGII 136
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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