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Conserved domains on  [gi|695714719|ref|WP_032644856|]
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MULTISPECIES: NADH-dependent FMN reductase RutF [Enterobacter]

Protein Classification

pyrimidine utilization flavin reductase protein F( domain architecture ID 10022704)

pyrimidine utilization flavin reductase protein F catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RutF TIGR03615
pyrimidine utilization flavin reductase protein F; This protein is observed in operons ...
 4-158 9.47e-96

pyrimidine utilization flavin reductase protein F; This protein is observed in operons extremely similar to that characterized in E. coli K-12 responsible for the import and catabolism of pyrimidines, primarily uracil. This protein is a member of the flavin reductase family defined by pfam01613. Presumably, this protein recycles the flavin of the RutA luciferase-like oxidoreductase.


:

Pssm-ID: 132654  Cd Length: 156  Bit Score: 273.60  E-value: 9.47e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719    4 DKQTFRDAMACVGAAVNIITTDGPAGMAGFTASAVCSVTDSPPTLLVCLNRGASVWPTFSENRTLCVNTLSAGQEPLSNL 83
Cdd:TIGR03615   2 DQQAFRDAMSRLGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRSASAYPAFKQNGTLCVNTLAAGQEDLSNL 81

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 695714719   84 FGGKTPMAERFTAARWQTGDTGCPRLEDALASFDCRISQVVSVGTHDILFCDIVSIIRHPAPQGLVWFDRSYHAL 158
Cdd:TIGR03615  82 FGGKTPMEERFAAGQWRTLVTGAPVLEDALVSFDCRISQSHSVGTHDILFCAVEAIAHSAGADALVYFNRRYHTL 156
 
Name Accession Description Interval E-value
RutF TIGR03615
pyrimidine utilization flavin reductase protein F; This protein is observed in operons ...
 4-158 9.47e-96

pyrimidine utilization flavin reductase protein F; This protein is observed in operons extremely similar to that characterized in E. coli K-12 responsible for the import and catabolism of pyrimidines, primarily uracil. This protein is a member of the flavin reductase family defined by pfam01613. Presumably, this protein recycles the flavin of the RutA luciferase-like oxidoreductase.


Pssm-ID: 132654  Cd Length: 156  Bit Score: 273.60  E-value: 9.47e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719    4 DKQTFRDAMACVGAAVNIITTDGPAGMAGFTASAVCSVTDSPPTLLVCLNRGASVWPTFSENRTLCVNTLSAGQEPLSNL 83
Cdd:TIGR03615   2 DQQAFRDAMSRLGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRSASAYPAFKQNGTLCVNTLAAGQEDLSNL 81

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 695714719   84 FGGKTPMAERFTAARWQTGDTGCPRLEDALASFDCRISQVVSVGTHDILFCDIVSIIRHPAPQGLVWFDRSYHAL 158
Cdd:TIGR03615  82 FGGKTPMEERFAAGQWRTLVTGAPVLEDALVSFDCRISQSHSVGTHDILFCAVEAIAHSAGADALVYFNRRYHTL 156
hpaC PRK15486
4-hydroxyphenylacetate 3-monooxygenase reductase subunit; Provisional
 1-159 1.64e-52

4-hydroxyphenylacetate 3-monooxygenase reductase subunit; Provisional


Pssm-ID: 185383  Cd Length: 170  Bit Score: 164.83  E-value: 1.64e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719   1 MTPDKQT--FRDAMACVGAAVNIITTDGPAGMAGFTASAVCSVTDSPPTLLVCLNRGASVWPTFSENRTLCVNTLSAGQE 78
Cdd:PRK15486   1 MQLDEQRlrFRDAMASLSAAVNIVTTAGDAGRCGITATAVCSVTDTPPSVMVCINANSAMNPVFQGNGKLCINVLNHEQE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719  79 PLSNLFGGKTPMA--ERFTAARWQTGDTGCPRLEDALASFDCRISQVVSVGTHDILFCDIVSIIRHPAPQGLVWFDRSYH 156
Cdd:PRK15486  81 LMARHFAGMTGMAmeERFSLSCWQKGPLGQPVLKGSLASLEGEISDVQTIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH 160


                 ...
gi 695714719 157 ALM 159
Cdd:PRK15486 161 PVM 163
RutF COG1853
FMN reductase RutF, DIM6/NTAB family [Energy production and conversion]; FMN reductase RutF, ...
 4-157 2.92e-50

FMN reductase RutF, DIM6/NTAB family [Energy production and conversion]; FMN reductase RutF, DIM6/NTAB family is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 441458  Cd Length: 160  Bit Score: 158.45  E-value: 2.92e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719   4 DKQTFRDAMACVGAAVNIITTDGPAG-MAGFTASAVCSVTDSPPTLLVCLNRGASVWPTFSENRTLCVNTLSAGQEPLSN 82
Cdd:COG1853    1 MPDAFRDALGRLAPGVAVVTTRDADGrPNGMTASSFTSVSLDPPLVLVSVAKSSDTLENIRETGEFVVNVLSEDQAELAN 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719  83 LFGGKT-PMAERFTAARWQT--GDTGCPRLEDALASFDCRISQVVSVGTHDILFCDIVSIIRHPAPQG--LVWFDRSYHA 157
Cdd:COG1853   81 RFAGRSgRGVDKFAGAGLTTasGEVGAPLLAEAPAWLECRVVDVIELGDHTLFIGEVVAVHVDEDVDGrpLLYLGGRYRR 160
Flavin_Reduct smart00903
Flavin reductase like domain; This entry represents the FMN-binding domain found in NAD(P) ...
 12-155 3.61e-50

Flavin reductase like domain; This entry represents the FMN-binding domain found in NAD(P)H-flavin oxidoreductases (flavin reductases), a class of enzymes capable of producing reduced flavin for bacterial bioluminescence and other biological processes. This domain is also found in various other oxidoreductase and monooxygenase enzymes... This domain consists of a beta-barrel with Greek key topology, and is related to the ferredoxin reductase-like FAD-binding domain. The flavin reductases have a different dimerisation mode than that found in the PNP oxidase-like family, which also carries an FMN-binding domain with a similar topology.


Pssm-ID: 214900  Cd Length: 147  Bit Score: 157.71  E-value: 3.61e-50
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719    12 MACVGAAVNIITTDGPAG-MAGFTASAVCSVTDSPPTLLVCLNRGASVWPTFSENRTLCVNTLSAGQEPLSNLFGGKTPm 90
Cdd:smart00903   1 LGRFPTGVAVVTTRDGDGgRVGLTASSFVSVSLDPPLVLVCVAKSSSTHPLIRESGRFVVNVLAEDQADLARRFAGKSG- 79

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 695714719    91 AERFTAARWQT---GDTGCPRLEDALASFDCRISQVVSVGTHDILFCDIVSIIRHPAPQGLVWFDRSY 155
Cdd:smart00903  80 ADRFEGVAWGLteaGVTGAPILAGALAWLECRVVQVIEVGDHTIFVGEVVAVHVRDDGEPLVYHRGGY 147
Flavin_Reduct pfam01613
Flavin reductase like domain; This is a flavin reductase family consisting of enzymes known to ...
 12-158 2.33e-42

Flavin reductase like domain; This is a flavin reductase family consisting of enzymes known to be flavin reductases as well as various oxidoreductase and monooxygenase components. VlmR is a flavin reductase that functions in a two-component enzyme system to provide isobutylamine N-hydroxylase with reduced flavin and may be involved in the synthesis of valanimycin. SnaC is a flavin reductase that provides reduced flavin for the oxidation of pristinamycin IIB to pristinamycin IIA as catalyzed by SnaA, SnaB heterodimer. This flavin reductase region characterized by enzymes of the family is present in the C-terminus of potential FMN proteins from Synechocystis sp. suggesting it is a flavin reductase domain.


Pssm-ID: 460266  Cd Length: 149  Bit Score: 138.18  E-value: 2.33e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719   12 MACVGAAVNIITTDGPAGMAGFTASAVCSVTDSPPTLLVCLNRGASVWPTFSENRTLCVNTLSAGQEPLSNLFGGKTPmA 91
Cdd:pfam01613   1 MRRFPTGVAVVTTDDGGEPNGMTVSSFTSVSLDPPLVLVSINRSSSTHDAIEASGEFVVNVLAEDQEELARRFAGRSG-R 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 695714719   92 ERFTAARWQTGDTGCPRLEDALASFDCRISQVVSVGTHDILFCDIVSI--IRHPAPQGLVWFDRSYHAL 158
Cdd:pfam01613  80 DKFAGITWVEGKVGAPLLKGALAALECRVVDTVDVGDHTLFIGEVVDVrvDEDADGEPLLYYRRRYRSL 148
 
Name Accession Description Interval E-value
RutF TIGR03615
pyrimidine utilization flavin reductase protein F; This protein is observed in operons ...
 4-158 9.47e-96

pyrimidine utilization flavin reductase protein F; This protein is observed in operons extremely similar to that characterized in E. coli K-12 responsible for the import and catabolism of pyrimidines, primarily uracil. This protein is a member of the flavin reductase family defined by pfam01613. Presumably, this protein recycles the flavin of the RutA luciferase-like oxidoreductase.


Pssm-ID: 132654  Cd Length: 156  Bit Score: 273.60  E-value: 9.47e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719    4 DKQTFRDAMACVGAAVNIITTDGPAGMAGFTASAVCSVTDSPPTLLVCLNRGASVWPTFSENRTLCVNTLSAGQEPLSNL 83
Cdd:TIGR03615   2 DQQAFRDAMSRLGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRSASAYPAFKQNGTLCVNTLAAGQEDLSNL 81

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 695714719   84 FGGKTPMAERFTAARWQTGDTGCPRLEDALASFDCRISQVVSVGTHDILFCDIVSIIRHPAPQGLVWFDRSYHAL 158
Cdd:TIGR03615  82 FGGKTPMEERFAAGQWRTLVTGAPVLEDALVSFDCRISQSHSVGTHDILFCAVEAIAHSAGADALVYFNRRYHTL 156
HpaC TIGR02296
4-hydroxyphenylacetate 3-monooxygenase, reductase component; This model identifies the ...
 8-159 1.19e-53

4-hydroxyphenylacetate 3-monooxygenase, reductase component; This model identifies the reductase component (HpaC) of 4-hydroxyphenylacetate 3-monooxygenase. This enzyme catalyzes the first step (hydroxylation at the 3-position) in the degradation of 4-hydroxyphenylacetate to succinate and pyruvate. 4-hydroxyphenylacetate arises from the degradation of tyrosine. These reductases catalyze the reduction of free flavins by NADPH. The flavin is then utilized by the large subunit of the monooxygenase.


Pssm-ID: 131349  Cd Length: 154  Bit Score: 166.86  E-value: 1.19e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719    8 FRDAMACVGAAVNIITTDGPAGMAGFTASAVCSVTDSPPTLLVCLNRGASVWPTFSENRTLCVNTLSAGQEPLSNLFGGK 87
Cdd:TIGR02296   1 FRDAMASLAAAVNIITTNGIAGKCGITATAVCSVTDTPPTVMVCINRNSAMNPIFQENGKLCINVLAHEQQEMAEHFAGM 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 695714719   88 TPMA--ERFTAARWQTGDTGCPRLEDALASFDCRISQVVSVGTHDILFCDIVSIIRHPAPQGLVWFDRSYHALM 159
Cdd:TIGR02296  81 TGLAmeERFAWHIWHEGPDGQPVLKGALAHLEGHIVDKHEIGTHTVFLVELKEIKVNGKRHALVYFRRQFKFVD 154
hpaC PRK15486
4-hydroxyphenylacetate 3-monooxygenase reductase subunit; Provisional
 1-159 1.64e-52

4-hydroxyphenylacetate 3-monooxygenase reductase subunit; Provisional


Pssm-ID: 185383  Cd Length: 170  Bit Score: 164.83  E-value: 1.64e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719   1 MTPDKQT--FRDAMACVGAAVNIITTDGPAGMAGFTASAVCSVTDSPPTLLVCLNRGASVWPTFSENRTLCVNTLSAGQE 78
Cdd:PRK15486   1 MQLDEQRlrFRDAMASLSAAVNIVTTAGDAGRCGITATAVCSVTDTPPSVMVCINANSAMNPVFQGNGKLCINVLNHEQE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719  79 PLSNLFGGKTPMA--ERFTAARWQTGDTGCPRLEDALASFDCRISQVVSVGTHDILFCDIVSIIRHPAPQGLVWFDRSYH 156
Cdd:PRK15486  81 LMARHFAGMTGMAmeERFSLSCWQKGPLGQPVLKGSLASLEGEISDVQTIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH 160


                 ...
gi 695714719 157 ALM 159
Cdd:PRK15486 161 PVM 163
RutF COG1853
FMN reductase RutF, DIM6/NTAB family [Energy production and conversion]; FMN reductase RutF, ...
 4-157 2.92e-50

FMN reductase RutF, DIM6/NTAB family [Energy production and conversion]; FMN reductase RutF, DIM6/NTAB family is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 441458  Cd Length: 160  Bit Score: 158.45  E-value: 2.92e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719   4 DKQTFRDAMACVGAAVNIITTDGPAG-MAGFTASAVCSVTDSPPTLLVCLNRGASVWPTFSENRTLCVNTLSAGQEPLSN 82
Cdd:COG1853    1 MPDAFRDALGRLAPGVAVVTTRDADGrPNGMTASSFTSVSLDPPLVLVSVAKSSDTLENIRETGEFVVNVLSEDQAELAN 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719  83 LFGGKT-PMAERFTAARWQT--GDTGCPRLEDALASFDCRISQVVSVGTHDILFCDIVSIIRHPAPQG--LVWFDRSYHA 157
Cdd:COG1853   81 RFAGRSgRGVDKFAGAGLTTasGEVGAPLLAEAPAWLECRVVDVIELGDHTLFIGEVVAVHVDEDVDGrpLLYLGGRYRR 160
Flavin_Reduct smart00903
Flavin reductase like domain; This entry represents the FMN-binding domain found in NAD(P) ...
 12-155 3.61e-50

Flavin reductase like domain; This entry represents the FMN-binding domain found in NAD(P)H-flavin oxidoreductases (flavin reductases), a class of enzymes capable of producing reduced flavin for bacterial bioluminescence and other biological processes. This domain is also found in various other oxidoreductase and monooxygenase enzymes... This domain consists of a beta-barrel with Greek key topology, and is related to the ferredoxin reductase-like FAD-binding domain. The flavin reductases have a different dimerisation mode than that found in the PNP oxidase-like family, which also carries an FMN-binding domain with a similar topology.


Pssm-ID: 214900  Cd Length: 147  Bit Score: 157.71  E-value: 3.61e-50
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719    12 MACVGAAVNIITTDGPAG-MAGFTASAVCSVTDSPPTLLVCLNRGASVWPTFSENRTLCVNTLSAGQEPLSNLFGGKTPm 90
Cdd:smart00903   1 LGRFPTGVAVVTTRDGDGgRVGLTASSFVSVSLDPPLVLVCVAKSSSTHPLIRESGRFVVNVLAEDQADLARRFAGKSG- 79

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 695714719    91 AERFTAARWQT---GDTGCPRLEDALASFDCRISQVVSVGTHDILFCDIVSIIRHPAPQGLVWFDRSY 155
Cdd:smart00903  80 ADRFEGVAWGLteaGVTGAPILAGALAWLECRVVQVIEVGDHTIFVGEVVAVHVRDDGEPLVYHRGGY 147
Flavin_Reduct pfam01613
Flavin reductase like domain; This is a flavin reductase family consisting of enzymes known to ...
 12-158 2.33e-42

Flavin reductase like domain; This is a flavin reductase family consisting of enzymes known to be flavin reductases as well as various oxidoreductase and monooxygenase components. VlmR is a flavin reductase that functions in a two-component enzyme system to provide isobutylamine N-hydroxylase with reduced flavin and may be involved in the synthesis of valanimycin. SnaC is a flavin reductase that provides reduced flavin for the oxidation of pristinamycin IIB to pristinamycin IIA as catalyzed by SnaA, SnaB heterodimer. This flavin reductase region characterized by enzymes of the family is present in the C-terminus of potential FMN proteins from Synechocystis sp. suggesting it is a flavin reductase domain.


Pssm-ID: 460266  Cd Length: 149  Bit Score: 138.18  E-value: 2.33e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 695714719   12 MACVGAAVNIITTDGPAGMAGFTASAVCSVTDSPPTLLVCLNRGASVWPTFSENRTLCVNTLSAGQEPLSNLFGGKTPmA 91
Cdd:pfam01613   1 MRRFPTGVAVVTTDDGGEPNGMTVSSFTSVSLDPPLVLVSINRSSSTHDAIEASGEFVVNVLAEDQEELARRFAGRSG-R 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 695714719   92 ERFTAARWQTGDTGCPRLEDALASFDCRISQVVSVGTHDILFCDIVSI--IRHPAPQGLVWFDRSYHAL 158
Cdd:pfam01613  80 DKFAGITWVEGKVGAPLLKGALAALECRVVDTVDVGDHTLFIGEVVDVrvDEDADGEPLLYYRRRYRSL 148
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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